A tissue sampling device for muscle cell culture

Abstract

The present invention provides a muscle cell culture tissue sampling device, which belongs to the field of cell culture technology. The technical solution is: comprising a sampling tube, a cutting mechanism and a sampling mechanism arranged on the sampling tube. The beneficial effects of the present invention are: simple structure, easy to use, capable of rapid sampling, and ensuring that the sample will not be contaminated to a great extent, thereby improving the efficiency and quality of scientific research.

Description

[0001] This case is a divisional application. The title of the invention of the original application is: A primary muscle cell culture tissue sampling device. The application date of the original application is: 2021-12-17, and the application number of the original application is: 202011491076.0. Technical Field

[0002] The invention relates to the technical field of cell culture, in particular to a muscle cell culture tissue sampling device. Background Art

[0003] As people's living standards continue to improve, the demand for meat in daily life increases. This type of food is rich in various nutrients. However, the growth rate of edible livestock such as pigs and donkeys is relatively slow. Under proper feeding and management conditions during the fattening period, they only gain an average of more than half a catty per day. Since the weight gain of organisms is achieved through cell proliferation, it is necessary to establish a livestock muscle cell culture system and study the mechanism of muscle cell proliferation to provide a scientific theoretical basis for increasing the daily weight gain of livestock.

[0004] To establish a livestock muscle cell culture system, it is first necessary to remove a small amount of muscle tissue from the livestock. Currently, sampling is done at the slaughterhouse. After the skin is peeled and the disembowelment is opened, a small amount of tissue is cut from the plump part of the muscle with scissors, and then placed in a sample bottle filled with saline or PBS with tweezers and taken back to the laboratory. Since most slaughterhouses are small workshops with relatively simple environmental conditions, and tissue sampling is an open process that cannot guarantee aseptic operation, the small amount of muscle tissue cut is likely to have brought in bacteria or other contaminants. When primary muscle cells are cultured, bacterial growth is almost inevitable, resulting in serious scientific research waste. Summary of the invention

[0005] The purpose of the present invention is to provide a muscle cell culture tissue sampling device which has a simple structure, is easy to use, can quickly take samples, and greatly ensures that the samples will not be contaminated, thereby improving the efficiency and quality of scientific research.

[0006] The present invention is achieved by the following measures:

[0007] A muscle cell culture tissue sampling device, characterized in that it comprises a sampling barrel, a cutting mechanism and a sampling mechanism arranged on the sampling barrel;

[0008] The sampling tube comprises a sampling inner sleeve and a protective outer sleeve, wherein the protective outer sleeve is sleeved on the periphery of the sampling inner sleeve, an annular buckle cover is arranged on the periphery of the top of the sampling inner sleeve, and the annular buckle cover is arranged on the periphery of the top of the protective outer sleeve, and a bell mouth is arranged on the bottom of the sampling inner sleeve;

[0009] The cutting mechanism comprises a conical bottom cylinder arranged at the bottom of the protective outer sleeve, the conical bottom cylinder is composed of a plurality of arc-shaped blades which are wide at the top and narrow at the bottom, and the plurality of arc-shaped blades are hingedly arranged on the outer side wall of the bottom of the protective outer sleeve;

[0010] A first U-shaped connecting plate is arranged at the middle position of the inner side of the plurality of arc-shaped blades, a connecting rod is arranged inside the protective outer sleeve, one end of the connecting rod is threadedly connected with a pull rod, and the other end of the connecting rod is arranged at the positions of the plurality of first U-shaped connecting plates corresponding to the plurality of first U-shaped connecting plates, a connecting rod is arranged between the corresponding first U-shaped connecting plates and the second U-shaped connecting plates, and the two ends of the connecting rod are respectively hingedly arranged on the first U-shaped connecting plate and the second U-shaped connecting plate;

[0011] Preferably, the edges of the arc-shaped blades are all provided with cutting edges, and the bottom surface of the connecting rod is provided with cutting edges.

[0012] Inclined blades are provided on the sides of both sides of the connecting rod;

[0013] The sampling mechanism includes the sampling inner sleeve and a pull rod arranged in the sampling inner sleeve, one end of the pull rod is connected to the connecting rod, and the other end of the pull rod passes through the sampling inner sleeve and is provided with a handle. The pull rod is located inside the sampling inner sleeve and is provided with a first material baffle plate, and the pull rod is provided with a second material baffle plate near the end position, and the second material baffle plate is arranged outside the sampling inner sleeve and against the inner side of the bell mouth.

[0014] The specific features of the present invention also include:

[0015] A plurality of fixed sleeves are arranged on the outer wall of the sampling outer sleeve, a pair of L-shaped support plates are arranged at the outer edge of the wider side of the arc-shaped blade, a rotating rod is arranged between the L-shaped support plates, and the rotating rod is arranged in the fixed sleeve;

[0016] A limit pin is arranged on one side of the fixed sleeve, and an arc-shaped limit groove for cooperating with the limit pin is opened on the L-shaped support plate on the same side, and the limit pin is arranged in the limit sliding groove.

[0017] A mounting threaded hole is provided at one end of the linkage rod connected to the pull rod, and a mounting stud is provided at the end of the pull rod, and the mounting stud is arranged in the threaded mounting hole.

[0018] A rubber cushion layer is arranged on the periphery of the second material baffle plate.

[0019] The method for using the sampling device comprises the following steps:

[0020] S1. Assemble the sampling device, first screw the mounting stud of the pull rod into the threaded mounting hole at the end of the connecting rod, then install the sampling inner sleeve in the protective outer sleeve, and buckle the annular buckle cover on the protective outer sleeve, while keeping the pull rod and the first baffle plate arranged inside the sampling inner sleeve;

[0021] S2, cut open the skin of the muscle to be sampled, take the assembled sampling device, push the pull rod downward, the pull rod pushes the plurality of arc-shaped blades to flip, until the plurality of arc-shaped blades stand upright, insert the plurality of arc-shaped blades into the muscle with the cut skin, continue to push and rotate the pull rod, the pull rod drives the arc-shaped blades to rotate and penetrate into the muscle, and drives the sampling tube into the muscle at the same time, during the process of entering the muscle, the muscle cut by the arc-shaped blades will enter between the sampling inner sleeve and the protective outer sleeve, and a small amount of muscle will also enter the sampling inner sleeve;

[0022] When the end of the sampling tube is 3-4 mm away from the muscle surface, stop pushing, then fix the sampling tube, pull the pull rod, so that the arc-shaped blades are closed to cut the muscle. During the closing process of the arc-shaped blades, the second baffle plate will push a small amount of muscle into the sampling inner sleeve. After the arc-shaped blades are closed, the second baffle plate will be against the inner wall of the bell mouth and squeezed into the bell mouth through the rubber pad layer. Pull the pull rod to pull the sampling tube out as a whole and separate it from the muscle, completing the preliminary sampling;

[0023] S3. Bring the entire sampling device after preliminary sampling to the laboratory, fix the sampling tube, gently push the pull rod to separate the second baffle plate from the inner wall of the bell mouth, rotate the pull rod to separate the pull rod from the connecting rod, pull the pull rod to pull out the sampling inner sleeve and separate it from the protective outer sleeve, then push the pull rod to push out the muscle in the sampling inner sleeve, take the muscle close to the second baffle plate side and put it into physiological saline to complete the sampling, and abandon the muscle close to the first baffle plate side.

[0024] The combined use of the protective outer sleeve and the sampling inner sleeve can ensure that the sample is not contaminated to a great extent.

[0025] The beneficial effects of the present invention are: simple structure, easy use, rapid sampling, and great guarantee that the sample will not be contaminated, thereby improving the efficiency and quality of scientific research. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic structural diagram of an embodiment of the present invention.

[0027] Figure 2It is a schematic structural diagram of an embodiment of the present invention.

[0028] Figure 3 Schematic diagram of the internal structure of an embodiment of the present invention.

[0029] Figure 4 It is a schematic structural diagram of an embodiment of the present invention.

[0030] Figure 5 for Figure 4 A partial enlarged view of part A in the middle.

[0031] Figure 6 It is a structural schematic diagram of a bottom view of an embodiment of the present invention.

[0032] Among them, the accompanying drawings are marked as: 1. Protective outer sleeve; 2. Conical bottom sleeve; 3. Ring buckle cover; 4. Pull rod; 5. Handle; 6. Arc-shaped blade; 7. L-shaped support plate; 8. Fixed sleeve; 9. Connecting rod; 10. Sampling inner sleeve; 11. First U-shaped connecting plate; 12. Second U-shaped connecting plate; 13. Connecting rod; 15. Mounting stud; 16. Second material stop plate; 17. Bell mouth; 18. First material stop plate; 19. Arc-shaped limit groove; 20. Limit pin. DETAILED DESCRIPTION

[0033] In order to clearly illustrate the technical features of this solution, this solution is described below through a specific implementation method.

[0034] See also Figure 1-6 , a muscle cell culture tissue sampling device, comprising a sampling barrel, a cutting mechanism and a sampling mechanism arranged on the sampling barrel;

[0035] The sampling tube includes a sampling inner sleeve 10 and a protective outer sleeve 1. The protective outer sleeve 1 is sleeved on the outer periphery of the sampling inner sleeve 10. An annular buckle cover 3 is arranged on the outer periphery of the top of the sampling inner sleeve 10. The annular buckle cover 3 is arranged on the outer periphery of the top of the protective outer sleeve 1. A bell mouth 17 is arranged on the bottom of the sampling inner sleeve 10.

[0036] The cutting mechanism comprises a conical bottom cylinder 2 arranged at the bottom of the protective outer sleeve 1, the conical bottom cylinder 2 is composed of a plurality of arc-shaped blades 6 which are wide at the top and narrow at the bottom, and the plurality of arc-shaped blades 6 are hingedly arranged on the outer side wall of the bottom of the protective outer sleeve 1;

[0037] A first U-shaped connecting plate 11 is provided at the middle position of the inner side of the plurality of arc-shaped blades 6, a connecting rod 9 is provided inside the protective outer sleeve 1, one end of the connecting rod 9 is threadedly connected to the pull rod 4, and the other end of the connecting rod 9 is provided with a plurality of second U-shaped connecting plates 12 corresponding to the positions of the plurality of first U-shaped connecting plates 11, a connecting rod 13 is provided between the corresponding first U-shaped connecting plates 11 and the second U-shaped connecting plates 12, and the two ends of the connecting rod 13 are respectively hingedly provided on the first U-shaped connecting plate 11 and the second U-shaped connecting plate 12;

[0038] Preferably, the edges of the arc-shaped blade 6 are all provided with cutting edges, and the bottom surface of the connecting rod 13 is provided with cutting edges.

[0039] The connecting rod 13 is provided with inclined blades on both sides;

[0040] The sampling mechanism includes a sampling inner sleeve 10 and a pull rod 4 arranged in the sampling inner sleeve 10, one end of the pull rod 4 is connected to the connecting rod 9, and the other end of the pull rod 4 passes through the sampling inner sleeve 10 and is provided with a handle 5. The pull rod 4 is located inside the sampling inner sleeve 10 and is provided with a first material baffle plate 18. The pull rod 4 is provided with a second material baffle plate 16 near the end position. The second material baffle plate 16 is arranged outside the sampling inner sleeve 10 and against the inner side of the bell mouth 17.

[0041] The specific features of the present invention also include:

[0042] A plurality of fixed sleeves 8 are arranged on the outer wall of the sampling outer sleeve, a pair of L-shaped support plates 7 are arranged at the outer edge of the wider side of the arc-shaped blade 6, a rotating rod is arranged between the L-shaped support plates 7, and the rotating rod is arranged in the fixed sleeve 8;

[0043] A limit pin 20 is arranged on one side of the fixed sleeve 8, and an arc-shaped limit groove 19 for cooperating with the limit pin 20 is opened on the L-shaped support plate 7 on the same side, and the limit pin 20 is arranged in the limit sliding groove.

[0044] A threaded mounting hole is provided at one end of the connecting rod 9 connected to the pull rod 4, and a mounting stud 15 is provided at the end of the pull rod 4, and the mounting stud 15 is arranged in the threaded mounting hole.

[0045] A rubber cushion layer is disposed on the periphery of the second baffle plate 16 .

[0046] The method of using the sampling device comprises the following steps:

[0047] S1. Assemble the sampling device. First, screw the mounting stud 15 of the pull rod 4 into the threaded mounting hole at the end of the connecting rod 9. Then, install the sampling inner sleeve 10 in the protective outer sleeve 1, and buckle the annular buckle cover 3 on the protective outer sleeve 1. Meanwhile, keep the pull rod 4 and the first baffle plate 18 arranged inside the sampling inner sleeve 10.

[0048] S2, cut the skin of the muscle to be sampled, take the assembled sampling device, push the pull rod 4 downward, the pull rod 4 pushes the plurality of curved blades 6 to flip, until the plurality of curved blades 6 stand upright, insert the plurality of curved blades 6 into the muscle with the cut skin, continue to push and rotate the pull rod 4, the pull rod 4 drives the curved blades 6 to rotate and penetrate into the muscle, and at the same time drives the sampling tube into the muscle, during the process of entering the muscle, the muscle cut by the curved blades 6 will enter between the sampling inner sleeve 10 and the protective outer sleeve 1, and a small amount of muscle will also enter the sampling inner sleeve 10;

[0049] When the end of the sampling tube is 3-4 mm away from the muscle surface, stop pushing, then fix the sampling tube, pull the pull rod 4, so that the arc blades 6 are closed to cut the muscle. During the closing process of the arc blades 6, the second baffle plate 16 will push a small amount of muscle into the sampling inner sleeve 10. After the arc blades 6 are closed, the second baffle plate 16 will be against the inner wall of the bell mouth 17 and squeezed into the bell mouth 17 through the rubber cushion layer. Pull the pull rod 4 to pull the sampling tube out as a whole and away from the muscle, completing the preliminary sampling.

[0050] S3. Bring the entire sampling device after preliminary sampling to the laboratory, fix the sampling tube, gently push the pull rod 4 to separate the second baffle plate 16 from the inner wall of the bell mouth 17, rotate the pull rod 4 to separate the pull rod 4 from the connecting rod 9, pull the pull rod 4 to pull out the sampling inner sleeve 10 and separate it from the protective outer sleeve 1, then push the pull rod 4 to push the muscle in the sampling inner sleeve 10 out, take the muscle close to the second baffle plate 16 side and put it into physiological saline to complete the sampling, and abandon the muscle close to the first baffle plate 18 side.

[0051] The combined use of the protective outer sleeve 1 and the sampling inner sleeve 10 can ensure that the sample is not contaminated to a great extent.

[0052] Technical features not described in the present invention can be achieved through or by adopting existing technologies and will not be described in detail here. Of course, the above description is not a limitation of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by ordinary technicians in this technical field within the essential scope of the present invention should also fall within the scope of protection of the present invention.

Claims

1. A muscle cell culture tissue sampling device, characterized in that: It comprises a sampling cylinder, a cutting mechanism and a sampling mechanism arranged on the sampling cylinder; The sampling tube comprises a sampling inner sleeve and a protective outer sleeve, wherein the protective outer sleeve is sleeved on the periphery of the sampling inner sleeve, an annular buckle cover is arranged on the periphery of the top of the sampling inner sleeve, and the annular buckle cover is arranged on the periphery of the top of the protective outer sleeve, and a bell mouth is arranged on the bottom of the sampling inner sleeve; The cutting mechanism comprises a conical bottom cylinder arranged at the bottom of the protective outer sleeve, the conical bottom cylinder is composed of a plurality of arc-shaped blades which are wide at the top and narrow at the bottom, and the plurality of arc-shaped blades are hingedly arranged on the outer side wall of the bottom of the protective outer sleeve; A first U-shaped connecting plate is arranged at the middle position of the inner side of the plurality of arc-shaped blades, a connecting rod is arranged inside the protective outer sleeve, one end of the connecting rod is threadedly connected with a pull rod, and the other end of the connecting rod is arranged at the positions of the plurality of first U-shaped connecting plates corresponding to the plurality of first U-shaped connecting plates, a connecting rod is arranged between the corresponding first U-shaped connecting plates and the second U-shaped connecting plates, and the two ends of the connecting rod are respectively hingedly arranged on the first U-shaped connecting plate and the second U-shaped connecting plate; The sampling mechanism comprises the sampling inner sleeve and a pull rod arranged in the sampling inner sleeve, one end of the pull rod is connected to the linkage rod, the other end of the pull rod passes through the sampling inner sleeve and is provided with a handle, the pull rod is located inside the sampling inner sleeve and is provided with a first material baffle plate, the pull rod is provided with a second material baffle plate near the end position, the second material baffle plate is arranged outside the sampling inner sleeve and abuts against the inner side of the bell mouth; A plurality of fixed sleeves are arranged on the outer wall of the sampling outer sleeve, a pair of L-shaped support plates are arranged at the outer edge of the wider side of the arc-shaped blade, a rotating rod is arranged between the L-shaped support plates, and the rotating rod is arranged in the fixed sleeve; A limit pin is arranged on one side of the fixed sleeve, and an arc-shaped limit groove for cooperating with the limit pin is opened on the L-shaped support plate on the same side, and the limit pin is arranged in the limit sliding groove. A threaded mounting hole is provided at one end of the connecting rod connected to the pull rod, and a mounting stud is provided at the end of the pull rod, and the mounting stud is arranged in the threaded mounting hole; A rubber cushion layer is arranged on the periphery of the second material baffle plate.

2. The muscle cell culture tissue sampling device according to claim 1, characterized in that: The method for using the sampling device comprises the following steps: S1. Assemble the sampling device, first screw the mounting stud of the pull rod into the threaded mounting hole at the end of the connecting rod, then install the sampling inner sleeve in the protective outer sleeve, and buckle the annular buckle cover on the protective outer sleeve, while keeping the pull rod and the first baffle plate arranged inside the sampling inner sleeve; S2, cut open the skin of the muscle to be sampled, take the assembled sampling device, push the pull rod downward, the pull rod pushes the plurality of arc-shaped blades to flip, until the plurality of arc-shaped blades stand upright, insert the plurality of arc-shaped blades into the muscle with the cut skin, continue to push and rotate the pull rod, the pull rod drives the arc-shaped blades to rotate and penetrate into the muscle, and drives the sampling tube into the muscle at the same time, during the process of entering the muscle, the muscle cut by the arc-shaped blades will enter between the sampling inner sleeve and the protective outer sleeve, and a small amount of muscle will also enter the sampling inner sleeve; When the end of the sampling tube is 3-4 mm away from the muscle surface, stop pushing, then fix the sampling tube, pull the pull rod, so that the arc-shaped blades are closed to cut the muscle. During the closing process of the arc-shaped blades, the second baffle plate will push a small amount of muscle into the sampling inner sleeve. After the arc-shaped blades are closed, the second baffle plate will be against the inner wall of the bell mouth and squeezed into the bell mouth through the rubber pad layer. Pull the pull rod to pull the sampling tube out as a whole and separate it from the muscle, completing the preliminary sampling; S3. Bring the entire sampling device after preliminary sampling to the laboratory, fix the sampling tube, gently push the pull rod to separate the second baffle plate from the inner wall of the bell mouth, rotate the pull rod to separate the pull rod from the connecting rod, pull the pull rod to pull out the sampling inner sleeve and separate it from the protective outer sleeve, then push the pull rod to push out the muscle in the sampling inner sleeve, take the muscle close to the second baffle plate side and put it into physiological saline to complete the sampling, and abandon the muscle close to the first baffle plate side.

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