A kind of enzyme preparation process

Abstract

The invention discloses an enzyme preparation process. In the enzyme preparation process, an enzyme preparation device is used to perform an enzyme preparation operation, and a control ring drives a mixing bowl to move up and out of the liquid surface in a state where the bowl mouth faces upward and is filled with fermentation liquid. When a control pin moves to a groove position with the control ring, the control pin is extended into the groove relative to a rotating shaft based on the dead weight of the mixing bowl, so that the mixing bowl is tilted toward the bottom of the tank, and the fermentation liquid in the mixing bowl is poured back into the fermentation tank, so that air can just dissolve in the fermentation liquid, so as to realize pouring oxygenation and improve the dissolved oxygen effect of the fermentation liquid.

Description

Technical Field

[0001] The present invention relates to the technical field of enzymes, and in particular to an enzyme preparation process. Background Art

[0002] Fermentation is an indispensable activity in nature as a biocatalyst. Enzymes play different roles in the human body in terms of regeneration, reproduction, decomposition, metabolism, etc. Among them, edible enzymes are currently widely used to enhance human immunity, promote detoxification, purify blood, digest food, maintain skin tissue regeneration, regulate biochemical reactions, and promote metabolism, so as to meet the human body's needs for enzymes in terms of health and nutritional benefits.

[0003] In the existing production process, edible enzymes generally use a variety of edible fruits and vegetables, seeds, mushrooms, botanicals and other materials as raw materials, and use the characteristics of microorganisms to naturally ferment to metabolize carbohydrates, proteins, lipids and other substances in the raw materials, and produce and accumulate a large number of required metabolites. However, in the existing production process, in order to make the raw materials ferment better, it is generally necessary to stir the raw materials constantly to ensure that the raw materials and oxygen can be fully mixed. This method is not only ineffective, but also easy to produce bubbles. It not only does not significantly improve the effect of oxygen dissolving in water, but the bubbles produced will also block the dissolution of oxygen. Summary of the invention

[0004] In view of the above problems, the present invention discloses an enzyme preparation process to overcome the above problems or at least partially solve the above problems.

[0005] The enzyme preparation process adopts an enzyme preparation device to perform enzyme preparation operation, and the enzyme preparation device includes a fermentation tank, a control ring, a mixing bowl, a control track, a rotating shaft and a control pin; the fermentation tank is provided with an inlet and an outlet, the control ring is located inside the fermentation tank and can reciprocate along the height direction of the fermentation tank; the control track is arranged along the height direction of the fermentation tank, and a groove is arranged on the first side of the control track, the position of the groove is higher than the height of the liquid surface and faces the radial direction of the fermentation tank; the rotating shaft is arranged along the radial direction of the fermentation tank, and one end is connected to the control ring, and the other end is connected to one end of the mixing bowl in rotation along the height direction of the fermentation tank; the control pin is connected to the rotating shaft in a sliding manner along the radial direction of the fermentation tank, one end extends out of the rotating shaft to slide in contact with the first side of the control track, and the other end is connected to the mixing bowl in rotation in the height direction of the fermentation tank; wherein, when the control pin maintains sliding contact with the first side, the mixing bowl is in a horizontal state; when the control pin slides in contact with the groove, the mixing bowl is tilted toward the tank bottom direction of the fermentation tank;

[0006] The enzyme preparation process specifically comprises the following steps:

[0007] Step S1, loading: adding materials for preparing the enzyme into the fermentation tank through the inlet, and sealing the inlet;

[0008] Step S2, primary stirring: the control ring drives the stirring bowl to move from the bottom of the fermentation tank to the top of the fermentation tank, thereby performing primary stirring on the fermentation liquid;

[0009] Step S3, pouring and oxygenation: the control ring drives the mixing bowl filled with fermentation liquid to rise out of the liquid surface, and drives the mixing bowl to continue to move toward the top until the control pin moves to the groove, and the mixing bowl is tilted and rotated relative to the rotating shaft toward the bottom of the tank under the action of its own weight, and the fermentation liquid in the mixing bowl is poured into the fermentation tank, so that air is dissolved in the fermentation liquid, and pouring and oxygenation is completed;

[0010] Step S5, secondary stirring: the control ring drives the stirring bowl to move from the top of the fermentation tank to the bottom of the tank, thereby performing secondary stirring on the fermentation liquid;

[0011] Step S7, unloading: repeat the above steps to ferment the material in the fermentation tank, and then take out the fermented material through the outlet for subsequent processing of the enzyme.

[0012] Preferably, the rotating shaft can drive the mixing bowl to rotate; during the primary stirring in step S2 and the secondary stirring in step S5, the rotating shaft drives the mixing bowl to flip and stir in the fermentation liquid.

[0013] Preferably, the control track is provided with a protrusion, and a straight side surface and an arc groove are provided on the second side surface, and the protrusion is located at the arc groove; the end of the rotating shaft that is in sliding contact with the control track is composed of a plurality of straight tracks connected in sequence along the circumferential direction, and a turning groove is provided between two adjacent straight tracks; the arc groove is located below the liquid level, and the second side surface is perpendicular to the first side surface; in the process of the rotating shaft reciprocating along the control track, when the straight track maintains sliding contact with the straight side surface, the rotating shaft reciprocates linearly along the height direction of the fermentation tank, and when the turning groove is connected to the protrusion, the rotating shaft rotates around the protrusion.

[0014] Preferably, the second side surface is provided with four arc-shaped grooves and corresponding four protrusions, and the end of the rotating shaft that is in sliding contact with the control track adopts a square structure composed of four straight tracks and the turning grooves are located at four corner positions.

[0015] Preferably, the enzyme preparation device further comprises a piston, and the mixing bowl is provided with a piston cavity and four channels; the piston is located in the piston cavity, can move back and forth, and divides the piston cavity into a first piston cavity and a second piston cavity; one end of the first channel is connected to the first piston cavity, and the other end extends to the outside of the bottom of the mixing bowl; one end of the second channel is connected to the first piston cavity, and the other end extends to the rim of the mixing bowl; one end of the third channel is connected to the second piston cavity, and the other end extends to the outside of the bottom of the mixing bowl; one end of the fourth channel is connected to the second piston cavity, and the other end extends to the rim of the mixing bowl;

[0016] The enzyme preparation process also includes the following steps:

[0017] Step S4, spraying to remove foam: after the pouring and oxygenation in step S3 is completed, the control ring drives the mixing bowl to move out of the liquid surface, controls the piston to move toward the first piston chamber, compresses the fermentation liquid in the first piston chamber and sprays it out through the first channel, and sprays and eliminates the foam on the liquid surface, and at the same time, the second piston chamber introduces air through the fourth channel;

[0018] Step S6, oxygenation in the liquid: after completing the secondary stirring in step S5, the piston is controlled to move toward the second piston chamber, the air in the second piston chamber is compressed and sprayed into the fermentation liquid through the third channel, and at the same time, the first piston chamber introduces the fermentation liquid through the second channel.

[0019] Preferably, the piston adopts a double piston rod structure, including an upper piston rod and a lower piston rod; the lower piston rod extends through the first piston cavity to the outside of the bottom of the mixing bowl, and the upper piston rod extends through the second piston cavity to the outside of the mouth of the mixing bowl.

[0020] Preferably, a first one-way valve is provided in the first channel to control the one-way outflow of the medium from the first piston chamber; a second one-way valve is provided in the second channel to control the one-way inflow of the medium from the first piston chamber; a third one-way valve is provided in the third channel to control the one-way outflow of the medium from the second piston chamber; and a fourth one-way valve is provided in the fourth channel to control the one-way inflow of the medium from the second piston chamber.

[0021] Preferably, a spray cavity and spray holes are provided at the bottom of the mixing bowl; the spray cavity is located inside the mixing bowl and is connected to the first channel and the third channel, and a plurality of spray holes are distributed at the bottom of the mixing bowl and are connected to the spray cavity.

[0022] Preferably, the enzyme preparation equipment includes a motor and a screw; the motor is fixed on the fermentation tank, one end of the screw is connected to the output end of the motor along the height direction of the fermentation tank, and the other end passes through the control ring and forms a transmission connection with the control ring.

[0023] Preferably, the enzyme preparation equipment includes a guide rod; the guide rod is arranged parallel to the screw rod, and passes through the control ring to form a guide sliding connection with the control ring.

[0024] The enzyme preparation process of the present invention is used to prepare the enzyme, which has the following beneficial technical effects:

[0025] 1. In the enzyme preparation process of the present invention, when the control ring drives the mixing bowl to move up and out of the liquid surface, the mixing bowl is filled with fermentation liquid with the bowl mouth facing upward. Then, when the control pin moves to the groove position with the control ring, based on the dead weight of the mixing bowl, the control pin extends into the groove relative to the rotating shaft, so that the mixing bowl is tilted toward the bottom of the tank, and then the fermentation liquid in the mixing bowl is poured back into the fermentation tank, so that the air can just dissolve in the fermentation liquid, realizing pouring oxygenation and improving the dissolved oxygen effect of the fermentation liquid.

[0026] 2. In the enzyme preparation process of the present invention, by arranging protrusions and arc grooves on the control track, and arranging straight tracks and turning grooves at the end of the rotating shaft, the control ring can drive the mixing bowl to move back and forth along the control track, thereby forming an overturning of the mixing bowl, and then forming an overturning and stirring of the fermentation liquid, thereby further improving the fermentation effect of the fermentation liquid.

[0027] 3. In the enzyme preparation process of the present invention, a piston and four channels are arranged in the mixing bowl, so that the fermentation liquid can be sprayed above the liquid level of the fermentation liquid toward the liquid surface foam to eliminate the foam, and high-pressure air can be output inside the fermentation liquid to oxygenate the fermentation liquid, thereby further improving the fermentation effect of the fermentation liquid.

[0028] 4. In the enzyme preparation process of the present invention, by designing the piston as a double piston rod structure and respectively arranging one-way valves in the four channels, the stirring, oxygenation and defoaming operations of the fermentation liquid can be achieved only when one motor-driven control ring moves back and forth in the fermentation tank, so that the enzyme preparation equipment has a compact structure and low cost, thereby making the control of the enzyme preparation process simpler and lower in cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the cross-sectional structure of the enzyme preparation equipment of this embodiment;

[0030] Figure 2 for Figure 1 Schematic diagram along the AA direction;

[0031] Figure 3 for Figure 1 Schematic diagram along the BB direction;

[0032] Figure 4 for Figure 1 Schematic diagram of the local structure at I in the middle;

[0033] Figure 5 When pouring the mixing bowl in this embodiment Figure 1 Schematic diagram of the local structure at I in the middle;

[0034] Figure 6 for Figure 3 Schematic diagram of the local structure at II in the middle;

[0035] Figure 7 In this embodiment, when the shaft rotates Figure 3 Schematic diagram of the local structure at II in the middle. DETAILED DESCRIPTION

[0036] The technical solution of the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments.

[0037] Combination Figures 1 to 7 As shown, the present embodiment discloses an enzyme preparation device, including a fermenter 1, a control ring 2, a mixing bowl 3, a control track 4, a rotating shaft 5 and a control pin 6. The fermenter 1 adopts a vertical tank body, with an inlet 7 at the upper end and an outlet 8 at the lower end. The control ring 2 is located inside the fermenter 1 and can reciprocate along the height direction of the fermenter 1. The control track 4 is arranged inside the fermenter 1 along the height direction of the fermenter 1, and a groove 411 is provided on the first side 41 of the control track 4, and the position of the groove 411 is higher than the liquid level of the fermentation liquid and faces the radial direction of the fermenter 1. The rotating shaft 5 is arranged along the radial direction of the fermenter 1, and one end is connected to the control ring 2, and the other end is rotatably connected to one end of the mixing bowl 3 along the height direction of the fermenter 1. The control pin 6 is also arranged along the radial direction of the fermenter 1 and is slidably connected to the rotating shaft 5. One end extends out of the rotating shaft 5 to slide in contact with the first side surface 41 of the control track 4, and the other end forms a rotational connection with the second point in the mixing bowl 3 along the height direction of the fermenter 1 through the connecting rod 61. When the control pin 6 maintains sliding contact with the first side surface 41, the mixing bowl 3 maintains a horizontal state. Conversely, when the control pin 6 moves to slide in contact with the groove 411, the mixing bowl 3 tilts toward the bottom of the fermenter 1.

[0038] At this time, when the enzyme preparation equipment of this embodiment is used to prepare the enzyme, by driving the control ring to move back and forth inside the fermentation tank, the mixing bowl can be driven to move up and down in the fermentation liquid to drive the fermentation liquid to form a mixed stirring, wherein when the mixing bowl moves up with the control ring and rises out of the liquid surface, the mixing bowl will be filled with fermentation liquid, and then when the control pin moves to the groove position with the control ring, due to the dead weight of the mixing bowl, the control pin extends into the groove relative to the rotating shaft, so that the mixing bowl is tilted toward the bottom of the tank, and then the fermentation liquid in the mixing bowl is poured back into the fermentation tank, so that the air can just dissolve in the fermentation liquid, thereby realizing pouring oxygenation and improving the dissolved oxygen effect of the fermentation liquid.

[0039] Combination Figure 3 , Figure 6 and Figure 7 As shown, in this embodiment, a protrusion 43 is further provided on the control track 4, and a straight side surface 421 and an arc groove 422 are provided on the second side surface 42, and the protrusion 43 is located at the arc groove 422. At the same time, one end of the rotating shaft 5 that is in sliding contact with the control track 4 is composed of four straight tracks 51 connected in sequence along the circumferential direction, and a turning groove 52 is provided between two adjacent straight tracks 51. The arc groove 422 is located at a position below the liquid level in the control track 4, and the second side surface 42 is perpendicular to the first side surface 41.

[0040] At this time, in the process of the reciprocating movement of the rotating shaft 5 along the control track 4, when the straight track 51 maintains sliding contact with the straight side surface 421, under the limiting guiding action between the straight side surface 421 and the straight track 51, the rotating shaft 5 reciprocates in a straight line along the height direction of the fermentation tank 1, and when the turning groove 52 moves to connect with the protrusion 43, the rotating shaft 5 rotates around the protrusion 43, thereby driving the mixing bowl 3 to flip over, forming a flipping and stirring of the fermentation liquid, thereby improving the stirring effect of the fermentation liquid.

[0041] Among them, in this embodiment, the end of the rotating shaft is designed to be a square structure, that is, it is composed of four straight tracks and four turning grooves, and four arc grooves and protrusions are set on the control track. In this way, the mixing bowl can be flipped four times a week in the fermentation liquid.

[0042] Of course, in other embodiments, the number of times the mixing bowl flips can be adjusted, for example, the end of the rotating shaft is designed to be a regular hexagon, and six arc grooves and protrusions are provided on the control track. Even when the end of the rotating shaft is designed to be a square structure, the number of arc grooves and protrusions on the control track can be increased to achieve the control of multiple flips of the mixing bowl.

[0043] In addition, in other embodiments, other methods may be used to drive the rotating shaft to rotate, such as separately providing a rotating motor, and using the rotating motor to control the reciprocating rotation of the rotating shaft, thereby controlling the flipping and stirring effect of the mixing bowl in the fermentation liquid.

[0044] Combination Figure 1 and Figure 4 As shown, a piston 9 is also provided in the enzyme preparation device of this embodiment, and a piston cavity and four channels are provided on the mixing bowl 3. The piston 9 is located in the piston cavity, can move back and forth, and divides the piston cavity into a first piston cavity 10 and a second piston cavity 11. One end of the first channel 121 is connected to the first piston cavity 10, and the other end extends to the outside of the bottom of the mixing bowl 3; one end of the second channel 122 is connected to the first piston cavity 10, and the other end extends to the mouth of the mixing bowl 3; one end of the third channel 123 is connected to the second piston cavity 11, and the other end extends to the outside of the bottom of the mixing bowl 3; one end of the fourth channel 124 is connected to the second piston cavity 11, and the other end extends to the mouth of the mixing bowl 3.

[0045] At this time, by controlling the reciprocating movement of the piston in the piston chamber, the pressure medium can be output with the help of the cooperation of the four channels. Among them, when the mixing bowl moves to a position above the liquid level, the piston is controlled to move in the direction of the first piston chamber, and the fermentation liquid in the first piston chamber is compressed by the piston and sprayed out through the first channel, so that the foam on the liquid surface is eliminated by shooting and blasting, achieving the effect of spraying and defoaming, and at the same time, the second piston chamber introduces air through the fourth channel. Conversely, when the mixing bowl moves to a position below the liquid level, the piston is controlled to move in the direction of the second piston chamber, and the air in the second piston chamber is compressed by the piston and sprayed out through the third channel, so that the air is sprayed into the fermentation liquid, achieving the effect of increasing oxygen in the liquid, and at the same time, the first piston chamber introduces the fermentation liquid through the second channel. In this way, the elimination of foam on the liquid surface and the effect of increasing oxygen in the fermentation liquid can be achieved.

[0046] Further, combined with Figure 4 As shown, in this embodiment, the piston 9 adopts a double piston rod structure, including a lower piston rod 91 and an upper piston rod 92. The lower piston rod 91 extends through the first piston chamber 10 to the outside of the bottom of the mixing bowl 3, and the upper piston rod 92 extends through the second piston chamber 11 to the outside of the bowl mouth of the mixing bowl 3.

[0047] At this time, when the mixing bowl moves to the top of the fermentation tank with the control ring, the contact between the upper piston rod and the top of the tank body is used to realize the movement of the piston toward the first piston chamber. Conversely, when the mixing bowl moves to the bottom of the fermentation tank with the control ring, the contact between the lower piston rod and the lower part of the tank body is used to realize the movement of the piston toward the second piston chamber, thereby realizing automatic drive control of the piston in the piston chamber.

[0048] Of course, in other embodiments, the piston may also be actively driven and controlled, for example, a telescopic rod is provided to be connected to the piston to drive the reciprocating movement of the piston in the piston chamber.

[0049] In addition, in the present embodiment, a one-way valve is respectively provided in the four channels, that is, a first one-way valve 131 is provided in the first channel 121 to control the medium to flow out of the first piston chamber 10 in one direction; a second one-way valve 132 is provided in the second channel 122 to control the medium to flow into the first piston chamber 10 in one direction; a third one-way valve 133 is provided in the third channel 123 to control the medium to flow out of the second piston chamber 11 in one direction; and a fourth one-way valve 134 is provided in the fourth channel 124 to control the medium to flow into the second piston chamber 11 in one direction.

[0050] At this time, as the medium pressure in the first piston chamber and the second piston chamber changes, the four channels can be switched on and off by means of corresponding one-way valves, thereby achieving the effect of automatic control. Of course, in other embodiments, according to different designs, a control valve, such as a reversing valve, can also be provided on the mixing bowl to actively switch the connection relationship of the four channels.

[0051] Furthermore, a spray cavity 14 and spray holes 15 are further provided at the bottom of the mixing bowl 3 of the present embodiment. The spray cavity 14 is located inside the mixing bowl 3 and is connected to the first channel 121 and the third channel 123, and the plurality of spray holes 15 are distributed at the bottom of the mixing bowl 3 and are connected to the spray cavity 14.

[0052] At this time, the pressure medium output through the first channel and the third channel first enters the spray chamber, and then is sprayed out through multiple spray holes facing different directions, thereby expanding the output range of the pressure medium and improving the effect of removing foam and increasing oxygen to the fermentation liquid.

[0053] Combination Figure 1 and Figure 2 As shown, the enzyme preparation device of this embodiment also includes a motor 16 and a screw 17. The motor 16 is fixed at the top of the fermentation tank 1, and the screw 17 is located inside the fermentation tank 1, and one end of the screw 17 is connected to the output end of the motor 16 along the height direction of the fermentation tank 1, and the other end passes through the control ring 2 and forms a transmission connection with the control ring 2. At this time, the drive motor 16 is reciprocated, and the control ring 2 can be driven to reciprocate along the height direction of the fermentation tank 1 through the screw 17.

[0054] In addition, three guide rods 18 are provided inside the fermenter 1. The guide rods 18 are arranged parallel to the screw rod 17, and pass through the control ring 2 to form a guide sliding connection with the control ring 2 along the height direction, thereby providing auxiliary guidance for the reciprocating movement of the control ring 2.

[0055] In this embodiment, four mixing bowls are provided on the control ring and are evenly distributed along the circumferential direction of the fermentation tank. Of course, in other embodiments, the number of mixing bowls can be adjusted according to the size of the fermentation tank and the size of the mixing bowl to meet the stirring effect of the fermentation liquid in the fermentation tank.

[0056] Combination Figures 1 to 7 As shown, the enzyme preparation device of this embodiment is used to prepare the enzyme, which specifically includes the following steps:

[0057] Step S1, loading: adding materials for preparing enzyme into the fermentation tank 1 through the inlet 7, and sealing the inlet 7.

[0058] Step S2, primary stirring: the control ring drives the stirring bowl to move from the bottom of the fermentation tank to the top of the fermentation tank, thereby performing primary stirring on the fermentation liquid.

[0059] With respect to the enzyme preparation device of the present embodiment, when the material for preparing the enzyme is added into the fermentation tank 1, the control ring 2 is located at the bottom of the fermentation tank 1, and the mixing bowl 3 is in a state where the mouth of the bowl faces upward. After the material is added, the start motor 16 drives the control ring 2 to start moving toward the top of the tank through the screw 17. When the control ring 2 drives the rotating shaft 5 to move along the straight side surface 421 of the second side surface 42 in the control track 4, the mixing bowl 3 moves in a direction with the mouth of the bowl facing upward under the limiting guiding action between the straight side surface 421 and the straight track 51 at the end of the rotating shaft 5. When the control ring 2 drives the rotating shaft 5 to move to the arc groove 422 of the second side surface 42 of the control track 4, the turning groove 52 at the end of the rotating shaft 5 forms a rotation connection with the protrusion 43, so that the rotating shaft 5 is turned 90°, and then the mixing bowl 3 is turned 90° in the fermentation liquid to stir the fermentation liquid. After that, when the control ring 2 drives the mixing bowl 3 to move in the pouring direction, it passes through the three arc grooves 422 and the corresponding protrusions 43 in the control track 4 in sequence, thereby driving the mixing bowl 3 to form four 90° turns to complete a round of turning and stirring operation. In this process, the control pin 6 always maintains a linear sliding connection with the first side surface 41 of the control track 4.

[0060] Step S3, pouring and oxygenation: the control ring drives the mixing bowl filled with fermentation liquid to rise out of the liquid surface, and drives the mixing bowl to continue to move toward the top until the control pin moves to the groove. The mixing bowl, under the action of its own weight, rotates in a pouring direction toward the bottom of the tank relative to the rotating shaft, and pours the fermentation liquid in the mixing bowl into the fermentation tank, so that the air dissolves in the fermentation liquid, and the pouring and oxygenation is completed.

[0061] For the enzyme preparation equipment of this embodiment, when the control ring 2 drives the mixing bowl 3 to move out the fermentation liquid with the opening facing upward and continue to move toward the top, when the control pin 6 moves to the groove 411 of the first side surface 41 in the control track 4, the mixing bowl 3 drives the control pin 6 to move relative to the rotating shaft 5 to extend into the groove 411 under the action of its own weight, thereby tilting the mixing bowl 3 toward the bottom of the tank, and then pouring the fermentation liquid in the mixing bowl 3 back into the fermentation tank 1, so that the air can just dissolve in the fermentation liquid, thereby achieving pouring and oxygenation.

[0062] Step S5, secondary stirring: the control ring drives the stirring bowl to move from the top of the fermentation tank to the bottom of the tank, thereby performing secondary stirring on the fermentation liquid.

[0063] For the enzyme preparation equipment of this embodiment, after the control ring 2 drives the mixing bowl 3 to the top of the fermentation tank 1, the motor 16 can be controlled to rotate in the opposite direction, and the control ring 2 is driven by the screw 17 to move toward the bottom of the tank again, thereby driving the mixing bowl 3 to enter the fermentation liquid again, and again passing through the four arc grooves 422 to control the mixing bowl 3 to flip four times and rotate in one circle, thereby forming a secondary stirring effect on the fermentation liquid.

[0064] Step S7, unloading: repeat the above steps to fully ferment the material in the fermentation tank 1, and then take out the fermented material through the outlet 8 for subsequent processing of the enzyme.

[0065] Preferably, the above enzyme preparation process further comprises the following steps:

[0066] Step S4, spraying to remove foam: After completing the pouring and oxygenation in step S3, the control ring drives the mixing bowl to move out of the liquid surface, and controls the piston to move toward the first piston chamber, compresses the fermentation liquid in the first piston chamber and sprays it out through the first channel, and the foam on the liquid surface is eliminated by spraying. At the same time, the second piston chamber introduces air through the fourth channel.

[0067] For the enzyme preparation equipment of this embodiment, after completing the pouring and oxygenation in step S3, when the control ring 2 drives the mixing bowl 3 to move to the top position, the upper piston rod 92 in the piston 9 forms contact with the top, and drives the piston 9 to move in the direction of the first piston chamber 10, thereby forming compression work on the fermentation liquid in the first piston chamber 10, and outputs it to the spray chamber 14 through the first one-way valve 131 in the first channel 121, and then sprays it to the foam on the liquid surface through the spray hole 15, so as to achieve the effect of eliminating the foam on the liquid surface by explosion. At the same time, as the volume of the second piston chamber 11 increases, the air above the liquid surface in the fermentation tank 1 enters the second piston chamber 11 through the fourth channel 124 and the fourth one-way valve 134.

[0068] Step S6, oxygenation in the liquid: after completing the secondary stirring in step S5, the piston is controlled to move toward the second piston chamber, the air in the second piston chamber is compressed and sprayed into the fermentation liquid through the fourth channel, and at the same time, the first piston chamber introduces the fermentation liquid through the second channel.

[0069] For the enzyme preparation equipment of this embodiment, after completing the secondary stirring in step S5, when the control ring 2 drives the mixing bowl 3 to move to the bottom of the tank, the lower piston rod 91 in the piston 9 forms contact with the bottom of the tank, and drives the piston 9 to move toward the second piston chamber 11, thereby forming a compression work on the air in the second piston chamber 11, and outputs it to the spray chamber 14 through the third one-way valve 133 in the third channel 123, and then injects it into the fermentation liquid through the spray hole 15, so as to achieve the effect of adding oxygen to the fermentation liquid. At the same time, as the volume of the first piston chamber 10 increases, the fermentation liquid in the fermentation tank 1 enters the first piston chamber 10 through the second channel 122 and the second one-way valve 132.

Claims

1. An enzyme preparation process, characterized in that: An enzyme preparation device is used to perform an enzyme preparation operation, the enzyme preparation device includes a fermentation tank, a control ring, a mixing bowl, a control track, a rotating shaft and a control pin; the fermentation tank is provided with an inlet and an outlet, the control ring is located inside the fermentation tank and can reciprocate along the height direction of the fermentation tank; the control track is arranged along the height direction of the fermentation tank, and a groove is arranged on the first side of the control track, the position of the groove is higher than the height of the liquid surface and faces the radial direction of the fermentation tank; the rotating shaft is arranged along the radial direction of the fermentation tank, and one end is connected to the control ring, and the other end is connected to one end of the mixing bowl in rotation along the height direction of the fermentation tank; the control pin is connected to the rotating shaft in a sliding manner along the radial direction of the fermentation tank, one end extends out of the rotating shaft to slide in contact with the first side of the control track, and the other end is connected to the mixing bowl in rotation in the height direction of the fermentation tank; wherein, when the control pin maintains sliding contact with the first side, the mixing bowl is in a horizontal state; when the control pin slides in contact with the groove, the mixing bowl is tilted toward the tank bottom of the fermentation tank; The enzyme preparation process specifically comprises the following steps: Step S1, loading: adding materials for preparing the enzyme into the fermentation tank through the inlet, and sealing the inlet; Step S2, primary stirring: the control ring drives the stirring bowl to move from the bottom of the fermentation tank to the top of the fermentation tank, thereby performing primary stirring on the fermentation liquid; Step S3, pouring and oxygenation: the control ring drives the mixing bowl filled with fermentation liquid to rise out of the liquid surface, and drives the mixing bowl to continue to move toward the top until the control pin moves to the groove, and the mixing bowl is tilted and rotated relative to the rotating shaft toward the bottom of the tank under the action of its own weight, and the fermentation liquid in the mixing bowl is poured into the fermentation tank, so that air is dissolved in the fermentation liquid, and pouring and oxygenation is completed; Step S5, secondary stirring: the control ring drives the stirring bowl to move from the top of the fermentation tank to the bottom of the tank, thereby performing secondary stirring on the fermentation liquid; Step S7, unloading: repeat the above steps to ferment the material in the fermentation tank, and then take out the fermented material through the outlet for subsequent processing of the enzyme.

2. The enzyme preparation process according to claim 1, characterized in that: The rotating shaft can drive the mixing bowl to rotate; during the primary stirring in step S2 and the secondary stirring in step S5, the rotating shaft drives the mixing bowl to flip and stir in the fermentation liquid.

3. The enzyme preparation process according to claim 2, characterized in that: The control track is provided with a protrusion, and a straight side surface and an arc groove are provided on the second side surface, and the protrusion is located at the arc groove; the end of the rotating shaft that is in sliding contact with the control track is composed of a plurality of straight tracks connected in sequence along the circumferential direction, and a turning groove is provided between two adjacent straight tracks; the arc groove is located below the liquid level, and the second side surface is perpendicular to the first side surface; during the reciprocating movement of the rotating shaft along the control track, when the straight track maintains sliding contact with the straight side surface, the rotating shaft reciprocates linearly along the height direction of the fermentation tank, and when the turning groove is connected to the protrusion, the rotating shaft rotates around the protrusion.

4. The enzyme preparation process according to claim 3, characterized in that: The second side surface is provided with four arc-shaped grooves and corresponding four protrusions. The end of the rotating shaft that is in sliding contact with the control track adopts a square structure, which is composed of four straight tracks and the turning grooves are located at four corner positions.

5. The enzyme preparation process according to claim 1, characterized in that: The enzyme preparation device also includes a piston, and the mixing bowl is provided with a piston cavity and four channels; the piston is located in the piston cavity, can move back and forth, and divides the piston cavity into a first piston cavity and a second piston cavity; one end of the first channel is connected to the first piston cavity, and the other end extends to the outside of the bottom of the mixing bowl; One end of the second channel is connected to the first piston chamber, and the other end extends to the mouth of the mixing bowl; One end of the third channel is communicated with the second piston chamber, and the other end extends to the outside of the bottom of the mixing bowl; One end of the fourth channel is connected to the second piston chamber, and the other end extends to the mouth of the mixing bowl; The enzyme preparation process also includes the following steps: Step S4, spraying to remove foam: after the pouring and oxygenation in step S3 is completed, the control ring drives the mixing bowl to move out of the liquid surface, controls the piston to move toward the first piston chamber, compresses the fermentation liquid in the first piston chamber and sprays it out through the first channel, and sprays and eliminates the foam on the liquid surface, and at the same time, the second piston chamber introduces air through the fourth channel; Step S6, oxygenation in the liquid: after completing the secondary stirring in step S5, the piston is controlled to move toward the second piston chamber, the air in the second piston chamber is compressed and sprayed into the fermentation liquid through the third channel, and at the same time, the first piston chamber introduces the fermentation liquid through the second channel.

6. The enzyme preparation process according to claim 5, characterized in that: The piston adopts a double piston rod structure, including an upper piston rod and a lower piston rod; the lower piston rod extends through the first piston cavity to the outside of the bottom of the mixing bowl, and the upper piston rod extends through the second piston cavity to the outside of the bowl mouth of the mixing bowl.

7. The enzyme preparation process according to claim 5, characterized in that: A first one-way valve is provided in the first channel to control the medium to flow out of the first piston chamber in one direction; a second one-way valve is provided in the second channel to control the medium to flow into the first piston chamber in one direction; a third one-way valve is provided in the third channel to control the medium to flow out of the second piston chamber in one direction; and a fourth one-way valve is provided in the fourth channel to control the medium to flow into the second piston chamber in one direction.

8. The enzyme preparation process according to claim 5, characterized in that: The mixing bowl has a spray cavity and spray holes at its bottom; the spray cavity is located inside the mixing bowl and is connected to the first channel and the third channel, and a plurality of spray holes are distributed at the mixing bowl bottom and are connected to the spray cavity.

9. The enzyme preparation process according to any one of claims 1 to 8, characterized in that: The enzyme preparation equipment includes a motor and a screw; the motor is fixed on the fermentation tank, one end of the screw is connected to the output end of the motor along the height direction of the fermentation tank, and the other end passes through the control ring and forms a transmission connection with the control ring.

10. The enzyme preparation process according to claim 9, characterized in that: The enzyme preparation equipment comprises a guide rod; the guide rod is arranged in parallel with the screw rod and passes through the control ring to form a guide sliding connection with the control ring.

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