A kind of Chinese soft-shelled turtle feed and preparation method thereof

Abstract

The present application relates to the technical field of Chinese soft-shelled turtle breeding, and discloses a Chinese soft-shelled turtle feed and a preparation method thereof. The ingredients of the Chinese soft-shelled turtle feed include cod meal, anchovy meal, black soldier fly meal, starch, soybean meal, minerals, multivitamins, coated vitamin C, emulsified fish oil and lactic acid bacteria, as well as fermentation products of water boat stink bugs and probiotic Bacillus, and fermentation products of cicada and bifidobacterium. Water boat stink bugs contain aliphatic substances with medium-length carbon chains and no branches. After water boat stink bugs are fermented by probiotic Bacillus, growth factors that can promote the growth and development of Chinese soft-shelled turtles are formed. There are proteins in cicadas that can be spun. After fermentation of cicadas and bifidobacteria, growth factors that can regulate hormone secretion in Chinese soft-shelled turtles are formed, promoting the growth and development of Chinese soft-shelled turtles. The combination of the above ingredients provides the nutrients needed for the growth of Chinese soft-shelled turtles, promotes the development of Chinese soft-shelled turtles, and improves the edible taste of Chinese soft-shelled turtles.

Description

Technical Field

[0001] The present application relates to the technical field of Chinese soft-shelled turtle breeding, and more specifically, to a Chinese soft-shelled turtle feed and a preparation method thereof. Background Art

[0002] Chinese soft-shelled turtle, also known as turtle or round fish, is a precious economic animal. It not only has delicious meat, rich nutrition and high protein content, but also is a high-end nutritional tonic, and has significant medicinal value. It has the function of nourishing yin, eliminating heat, breaking up lumps and softening hard masses.

[0003] All parts of the Chinese soft-shelled turtle can be used as medicine, which has the effects of nourishing yin and clearing heat, calming the liver and replenishing qi, breaking up knots and softening hard masses, and eliminating stasis. In order to meet the market demand for Chinese soft-shelled turtles for food and medical treatment, artificial breeding of Chinese soft-shelled turtles has been carried out on a large scale.

[0004] However, some current Chinese soft-shell turtle feeds cannot meet the nutrients required by Chinese soft-shell turtles during their growth process, resulting in insufficient development of Chinese soft-shell turtles and affecting the taste of food. Summary of the invention

[0005] In view of the fact that some Chinese soft-shelled turtle feeds cannot meet the nutrients required by the Chinese soft-shelled turtle during its growth, resulting in incomplete development of the Chinese soft-shelled turtle and affecting the taste when eaten, the present invention proposes a Chinese soft-shelled turtle feed and a preparation method thereof. The Chinese soft-shelled turtle feed can better meet the nutrients required by the Chinese soft-shelled turtle during its growth. Feeding the feed can promote the full growth and development of the Chinese soft-shelled turtle and has a good taste when eaten.

[0006] In the first aspect, the present application proposes a Chinese soft-shelled turtle feed and adopts the following technical solution.

[0007] A Chinese soft-shelled turtle feed, the ingredients of which include cod meal, anchovy meal, black soldier fly meal, starch, soybean meal, minerals, complex vitamins, coated vitamin C, emulsified fish oil and lactic acid bacteria, as well as fermentation products of water boatmen and probiotic bacillus, and fermentation products of katydid and bifidobacterium.

[0008] By adopting the above technical scheme, cod contains globulin, albumin, etc., and also contains various amino acids necessary for the development of Chinese soft-shelled turtles, as well as nutrients such as vitamin A, vitamin D, calcium, magnesium, and selenium. It is easily digested and absorbed by Chinese soft-shelled turtles, and can also improve acute and chronic enteritis. Anchovies are rich in crude protein and crude fat, contain a variety of essential amino acids for the growth of Chinese soft-shelled turtles, contain mineral elements such as Na, K, P, Mg, Fe, Zn, A1, Mn, B, Cu, Pb, Sn, Cr, and have high content of trace elements Fe and Zn, and have high edible and health value. Black soldier flies are rich in natural antibiotics, high in crude protein and fat, rich in mineral elements and vitamins, and are an excellent protein raw material. Black soldier fly powder is applied to Chinese soft-shelled turtle feed, which has the effects of improving the body's immunity, enhancing intestinal peristalsis, anti-inflammatory and anti-stress, sedative and analgesic, and promoting blood circulation. Starch has the function of combining other components and shaping. Soybean meal is rich in organic matter and a variety of trace elements. The Chinese soft-shelled turtle feed is also supplemented with minerals and multiple vitamins to supplement nutrition. Minerals can be added in the form of salt, eggshell powder, calcium carbonate, gypsum and potassium phosphate. Emulsified fish oil is easy to digest and absorb, making the Chinese soft-shelled turtle meat tender and improving the taste. Lactic acid bacteria ferment carbohydrates to produce lactic acid, which can adjust pH, prevent the growth of miscellaneous bacteria, and promote the growth and development of Chinese soft-shelled turtles. Water boatmen contain aliphatic substances with medium-length carbon chains and no branches, such as acids, aldehydes, ketones, alcohols and esters, crude protein, crude fat, etc., such as myristic acid, palmitic acid, linoleic acid, erucic acid, antimicrobial peptides, 6-oxo-2-hexenal, acetate trans-2-decene, etc. After water boatmen are fermented by probiotic Bacillus, growth factors that can promote the growth and development of Chinese soft-shelled turtles are formed. There are proteins in the body of the knitting girl that can be spun. After the knitting girl and bifidobacteria are fermented, growth factors that can regulate the secretion of hormones in the body of the Chinese soft-shelled turtle are generated, promoting the growth and development of the Chinese soft-shelled turtle. The fermentation products of water boatmen and probiotic Bacillus, as well as the fermentation products of kibbutz and bifidobacteria, work together to promote the growth and development of Chinese soft-shelled turtles and improve the taste of Chinese soft-shelled turtles. The above ingredients work together to provide the nutrients needed for the growth of Chinese soft-shelled turtles, promote the development of Chinese soft-shelled turtles, and improve the taste of Chinese soft-shelled turtles.

[0009] As an improvement of the Chinese soft-shelled turtle feed, the Chinese soft-shelled turtle feed includes, by mass, 39 to 44 parts of cod meal, 16 to 25 parts of anchovy meal, 18 to 22 parts of black soldier fly powder, 20 to 23 parts of starch, 3 to 8 parts of soybean meal, 1 to 4 parts of minerals, 0.2 to 0.5 parts of multivitamins, 0.1 to 0.3 parts of enveloped vitamin C, 5 to 15 parts of emulsified fish oil, 0.1 to 0.4 parts of lactic acid bacteria, 5 to 10 parts of fermentation products of water boatmen and probiotic bacillus, and 5 to 10 parts of fermentation products of cicadas and bifidobacteria.

[0010] By adopting the above technical scheme, the ratio of each nutrient meets the nutritional requirements of the growth and development of the Chinese soft-shelled turtle. Feeding the Chinese soft-shelled turtle with feed made from raw materials with this ratio can improve the physique of the Chinese soft-shelled turtle and promote its growth and development.

[0011] As an improvement of the Chinese soft-shelled turtle feed, the Chinese soft-shelled turtle feed includes, by mass, 41 to 42 parts of cod meal, 20 to 21 parts of anchovy meal, 19 to 20 parts of black soldier fly meal, 21 to 22 parts of starch, 5 to 6 parts of soybean meal, 2 to 3 parts of minerals, 0.3 to 0.4 parts of multivitamins, 0.1 to 0.2 parts of enveloped vitamin C, 9 to 11 parts of emulsified fish oil, 0.2 to 0.3 parts of lactic acid bacteria, 7 to 8 parts of fermentation products of water boatmen and probiotic bacillus, and 7 to 8 parts of fermentation products of cicadas and bifidobacteria.

[0012] By adopting the above technical solution, the ratio of each nutrient is further optimized, which can better promote the physical improvement and growth and development of Chinese soft-shelled turtle.

[0013] As an improvement of the Chinese soft-shelled turtle feed, the Chinese soft-shelled turtle feed also contains 0.5 to 1 portion of taurine.

[0014] By adopting the above technical solution, taurine can promote the growth and development of the Chinese soft-shelled turtle's nervous system and cell proliferation and differentiation, improve the physical fitness of the Chinese soft-shelled turtle, and promote the growth and development of the Chinese soft-shelled turtle.

[0015] As an improvement of the Chinese soft-shelled turtle feed, the compound vitamin includes vitamin A, vitamin D, vitamin E, vitamin K3, vitamin B 12 , vitamin B6, vitamin B2, vitamin B1, niacin, folic acid and pantothenic acid. Among them, the content of vitamin D can be 400-500μg / 100g, the content of vitamin E can be 500-3000μg / 100g, the content of vitamin K3 can be 100-200μg / 100g, and the content of vitamin B 12 The content of vitamin B6 can be 200-300 μg / 100g, the content of vitamin B6 can be 100-200 mg / 100g, the content of vitamin B2 can be 50-150 mg / 100g, the content of vitamin B1 can be 50-150 mg / 100g, the content of niacin can be 800-1300 mg / 100g, the content of folic acid can be 1-20 mg / 100g, and the content of pantothenic acid can be 200-500 mg / 100g. For example, a multivitamin contains vitamin D400 μg / 100g, vitamin E content 1000 μg / 100g, vitamin K3 content 150 μg / 100g, vitamin B 12The content of vitamin B6 is 250 μg / 100g, the content of vitamin B2 is 100 mg / 100g, the content of vitamin B1 is 100 mg / 100g, the content of niacin is 1000 mg / 100g, the content of folic acid is 10 mg / 100g, and the content of pantothenic acid is 300 mg / 100g. The multivitamin can also use commercially available multivitamins.

[0016] By adopting the above technical solution, the multivitamin contains multiple essential vitamins for the growth and development of the Chinese soft-shelled turtle, thereby promoting the growth and development of the Chinese soft-shelled turtle.

[0017] As an improvement of the Chinese soft-shelled turtle feed, the raw materials of the emulsified fish oil include anchovy oil, krill oil, an emulsifier and water.

[0018] By adopting the above technical scheme, anchovy oil is rich in polyunsaturated fatty acids, vitamins, and minerals, is easy to absorb, and has antioxidant, anti-inflammatory, and blood circulation promoting effects. Krill oil contains anti-inflammatory fatty acids, which contribute to the development of nerves and bones. The emulsifier can be lecithin, which can disperse anchovy oil and krill oil into tiny particles and disperse them in water, which are easy to digest and absorb. Calculated by mass percentage, the emulsified fish oil can include 40% anchovy oil, 30% krill oil, 3% emulsifier and 27% water. Compared with ordinary fish oil feed additives, the emulsified fish oil product will have better palatability, higher absorption rate, and more stable storage quality, and can better promote the absorption of fat-soluble vitamins in feed.

[0019] As an improvement of the Chinese soft-shelled turtle feed, the Chinese soft-shelled turtle feed also includes 2 to 6 portions of squid liver powder, 0.2 to 0.5 portions of

[0020] Choline chloride and 1 to 3 portions of the raw maternal slices.

[0021] By adopting the above technical scheme, squid liver powder contains a variety of nutrients, which have the effects of protecting eyes, nourishing blood and calming nerves; choline chloride can reduce the risk of fatty liver and cirrhosis; raw mother slices can boost appetite and improve indigestion. The three work together to promote the growth and development of Chinese soft-shelled turtle.

[0022] In the second aspect, the present application also proposes a method for preparing Chinese soft-shelled turtle feed, and adopts the following technical solution.

[0023] A method for preparing the Chinese soft-shelled turtle feed as described above, the preparation method comprising:

[0024] Grind the water boatman, add probiotic bacillus and water, the mass ratio of water boatman: probiotic bacillus: water can be (20-30): (0.05-0.2): (5-15), mix the three, maintain the pH at 6.5-7.5 at 35-38° C. for 1-2 days to obtain the fermentation product of the water boatman and the probiotic bacillus;

[0025] Grind the katabasis, add bifidobacteria and water, the mass ratio of katabasis: bifidobacteria: water can be (30-40): (0.1-0.2): (10-20), mix the three, seal, and ferment for 8-16 hours at 36-40° C. while maintaining the pH at 6.5-7.0 to obtain the fermentation product of the katabasis and bifidobacteria;

[0026] The cod meal, the anchovy meal, the black soldier fly meal, the starch, the soybean meal, the minerals, the multivitamin, the enveloped vitamin C, the lactic acid bacteria, the fermentation product of the water boatman and probiotic bacillus, and the fermentation product of the katydid and bifidobacterium are mixed, crushed, sieved, and mixed with water to form a jelly;

[0027] The colloid is placed in the equipment, the air pressure in the equipment is adjusted to 1-2 MPa, the colloid is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 4-6 minutes, and finally the surface of the expanded particles after insulation is covered with the emulsified fish oil to obtain the Chinese soft-shelled turtle feed.

[0028] By adopting the above technical scheme, the fermentation products of water boatmen and probiotic bacillus, and the fermentation products of knitting and bifidobacterium have a better effect on improving the growth and development of Chinese soft-shelled turtles. Among them, the coated vitamin C can be double-coated with vitamin C by embedding technology, so that vitamin C is isolated from the external environment, which effectively solves the problems caused by the characteristics of vitamin C being easily soluble in water and easily oxidized. The activity of vitamin C added to the feed can be fully kept stable without being oxidized. By making the remaining raw materials except emulsified fish oil into a colloid, pressurizing the colloid to 1-2MPa, and then extruding it to a normal pressure environment, the water in the colloid evaporates quickly, and the extruded particles expand rapidly to become puffed particles, reducing the feed density. Finally, the emulsified fish oil is covered on the surface of the puffed particles, so that the puffed particles can float in the water for Chinese soft-shelled turtles to eat. The Chinese soft-shelled turtle feed is easy to digest and absorb, has little pollution to the water body, is easy to mobilize the appetite of Chinese soft-shelled turtles, and improves the growth and development speed of Chinese soft-shelled turtles.

[0029] As an improvement of the preparation method of the Chinese soft-shelled turtle feed, the soybean meal is enzymatically hydrolyzed soybean meal, which is obtained by adding endo-enzyme, terminal peptidase and flavor enzyme into the soybean meal to decompose the soybean meal and hydrolyze the soybean protein into peptone, polypeptide and amino acid.

[0030] By adopting the above technical scheme, the enzymatically hydrolyzed soybean meal reduces the content of anti-nutritional factors in the soybean meal, increases its protein and small peptide content, and improves the nutritional value of the soybean meal; in addition, the enzymatically hydrolyzed soybean meal also has the characteristics of simple preparation, easy use and low cost.

[0031] As an improvement to the method for preparing the Chinese soft-shelled turtle feed, thiol protease is also added in the process of preparing the fermentation product of the water boatman and the probiotic bacillus.

[0032] By adopting the above technical scheme, the water boat contains aliphatic substances with medium carbon chain length and no branches, such as acids, aldehydes, ketones, alcohols and esters, crude protein, crude fat, etc., such as myristic acid, palmitic acid, linoleic acid, erucic acid, antimicrobial peptides, 6-oxo-trans-2-hexenal, acetate trans-2-decene, etc. After the water boat is fermented by probiotic Bacillus, a growth factor that can promote the growth and development of Chinese soft-shelled turtle is formed. Thiol protease is a type of proteolytic enzyme that contains a sulfhydryl group in the active center and relies on the sulfhydryl group to catalyze the hydrolysis of peptide bonds. It can catalyze the hydrolysis of proteins in water boats. After adding the sulfhydryl protease, the fermentation product of the water boat and the probiotic Bacillus can better promote the growth and development of Chinese soft-shelled turtle. The added mass fraction of sulfhydryl protease can be 0.5 to 1 part.

[0033] In summary, the Chinese soft-shelled turtle feed and the preparation method thereof of the present application have the following beneficial effects:

[0034] Cod contains globulin, albumin, etc., as well as various amino acids necessary for the development of Chinese soft-shelled turtles, as well as nutrients such as vitamin A, vitamin D, calcium, magnesium, and selenium. It can also improve acute and chronic enteritis; anchovies are rich in crude protein and crude fat, contain a variety of essential amino acids for the growth of Chinese soft-shelled turtles, and are rich in mineral elements; black soldier flies are rich in natural antibiotics, mineral elements, and vitamins, which can improve the body's immunity, enhance intestinal motility, resist inflammation and stress, calm and relieve pain, and promote blood circulation. Starch has the function of combining other components and shaping. Soybean meal is rich in organic matter and a variety of trace elements. The Chinese soft-shelled turtle feed also supplements minerals and multiple vitamins to supplement nutrition. Emulsified fish oil is easy to digest and absorb, making the Chinese soft-shelled turtle meat tender and improving the taste. Lactic acid bacteria ferment carbohydrates to produce lactic acid, which can adjust pH, prevent the growth of miscellaneous bacteria, and promote the growth and development of Chinese soft-shelled turtles. Water boat stink bugs contain aliphatic substances with medium-length carbon chains and no branches, such as acids, aldehydes, ketones, alcohols and esters, crude proteins, crude fats, etc., such as myristic acid, palmitic oleic acid, linoleic acid, erucic acid, antimicrobial peptides, 6-oxo-trans-2-hexenal, acetate trans-2-decene, etc. After being fermented by probiotic Bacillus, water boat stink bugs form growth factors that can promote the growth and development of Chinese soft-shelled turtles. The cicada contains textile proteins that can spray and weave nests. After fermentation with bifidobacteria, it forms growth factors that can regulate the hormone secretion in the Chinese soft-shelled turtle, promoting the growth and development of Chinese soft-shelled turtles. The fermentation products of water boat stink bugs and probiotic Bacillus, as well as the fermentation products of cicadas and bifidobacteria, work together to promote the growth and development of Chinese soft-shelled turtles and improve the edible taste of Chinese soft-shelled turtles. The above ingredients work together to provide the nutrients needed for the growth of Chinese soft-shelled turtles, promote the development of Chinese soft-shelled turtles, and improve the edible taste of Chinese soft-shelled turtles. DETAILED DESCRIPTION

[0035] The following is a detailed description of some embodiments of Chinese soft-shelled turtle feed and its preparation method.

[0036] The minerals used in the following examples and comparative examples are composed, by mass percentage, of 20% salt, 25% eggshell powder, 25% calcium carbonate, 15% gypsum and 15% potassium phosphate.

[0037] The compound vitamins used in the following examples and comparative examples include 400 μg / 100g of vitamin D, 1000 μg / 100g of vitamin E, 150 μg / 100g of vitamin K3, and 150 μg / 100g of vitamin B. 12 The content is 250μg / 100g, the content of vitamin B6 is 150mg / 100g, the content of vitamin B2 is 100mg / 100g, the content of vitamin B1 is 100mg / 100g, the content of niacin is 1000mg / 100g, the content of folic acid is 10mg / 100g, and the content of pantothenic acid is 300mg / 100g.

[0038] The emulsified fish oil used in the following examples and comparative examples includes, by mass percentage, 40% anchovy oil, 30% krill oil, 3% emulsifier and 27% water.

[0039] Example 1

[0040] This embodiment prepares a Chinese soft-shelled turtle feed, and the preparation method thereof is as follows:

[0041] 25 parts of water boatmen are taken by weight, ground, 0.1 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of the water boatmen and the probiotic bacillus.

[0042] Take 35 parts of katydid by mass, grind the katydid, add 0.1 parts of bifidobacterium and 15 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0043] The invention discloses a method for preparing a colloid product. The method comprises mixing 41 parts of cod meal, 20 parts of defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.2 parts of lactic acid bacteria, 7 parts of fermentation products of water boatmen and probiotic bacillus, and 7 parts of fermentation products of cicada and bifidobacterium, and then grinding the mixture and passing the mixture through a 60-mesh sieve. The powder particles passing through the sieve are added into 50 parts of water and mixed evenly to prepare a colloid product.

[0044] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0045] Example 2

[0046] This embodiment prepares a Chinese soft-shelled turtle feed, and the preparation method thereof is as follows:

[0047] 20 parts of water boatmen are taken by weight, ground, 0.2 parts of probiotic bacillus and 15 parts of water are added, mixed, and fermented for 2 days at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of water boatmen and probiotic bacillus.

[0048] Take 40 parts of katydid by mass, grind the katydid, add 0.2 parts of bifidobacterium and 10 parts of water, mix well and seal, ferment at 36-40°C with pH maintained at 6.5-7.0 for 16 hours to obtain a fermentation product of katydid and bifidobacterium.

[0049] The invention discloses a method for preparing a colloid product. The method comprises mixing 42 parts of cod meal, 21 parts of fully defatted anchovy meal, 20 parts of black soldier fly meal, 22 parts of pregelatinized starch, 6 parts of enzymolysis soybean meal, 3 parts of minerals, 0.4 parts of multivitamins, 0.2 parts of enveloped vitamin C, 0.3 parts of lactic acid bacteria, 8 parts of fermentation products of water boatmen and probiotic bacillus, and 8 parts of fermentation products of cicada and bifidobacterium, and then grinding the mixture and passing the mixture through a 60-mesh sieve. The powder particles passing through the sieve are added into 50 parts of water and mixed evenly to prepare a colloid product.

[0050] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain puffed particles, the puffed particles are kept warm at 35-40° C. for 5 minutes, and finally 11 parts of emulsified fish oil are evenly covered on the surface of all the puffed particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0051] Example 3

[0052] 30 parts of water boatmen are taken by weight, ground, 0.05 parts of probiotic bacillus and 5 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of water boatmen and probiotic bacillus.

[0053] Take 30 parts of katydid by mass, grind the katydid, add 0.15 parts of bifidobacterium and 20 parts of water, mix well and seal, ferment at 36-40°C with pH maintained at 6.5-7.0 for 8 hours to obtain a fermentation product of katydid and bifidobacterium.

[0054] The invention discloses a method for preparing a colloid product. The method comprises mixing 39 parts of cod meal, 25 parts of defatted anchovy meal, 18 parts of black soldier fly meal, 23 parts of pregelatinized starch, 3 parts of enzymatically hydrolyzed soybean meal, 4 parts of minerals, 0.2 parts of multivitamins, 0.3 parts of enveloped vitamin C, 0.4 parts of lactic acid bacteria, 5 parts of fermentation products of water boatmen and probiotic bacillus, and 10 parts of fermentation products of cicada and bifidobacterium, and then grinding the mixture and passing the mixture through a 60-mesh sieve. The powder particles passing through the sieve are added into 50 parts of water and mixed evenly to prepare a colloid product.

[0055] Put the jelly into an extruder, adjust the air pressure in the extruder to 1 MPa, stir the jelly at a temperature of 35-40° C., extrude it into the atmosphere to obtain expanded particles, keep the expanded particles at 35-40° C. for 4 minutes, and finally evenly cover the surfaces of all the expanded particles after insulation with 5 portions of emulsified fish oil to obtain Chinese soft-shelled turtle feed.

[0056] Example 4

[0057] 25 parts of water boatmen are taken by weight, ground, 0.08 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of water boatmen and probiotic bacillus.

[0058] Take 38 parts of katydid by mass, grind the katydid, add 0.17 parts of bifidobacterium and 13 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0059] The invention discloses a method for preparing a colloid product. The method comprises mixing 44 parts of cod meal, 16 parts of fully defatted anchovy meal, 22 parts of black soldier fly meal, 20 parts of pregelatinized starch, 8 parts of enzymatically hydrolyzed soybean meal, 1 part of minerals, 0.5 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.1 parts of lactic acid bacteria, 10 parts of fermentation products of water boatmen and probiotic bacillus, and 5 parts of fermentation products of cicada and bifidobacterium, and then grinding the mixture, passing through a 60-mesh sieve, adding 50 parts of water to the powder particles passing through the sieve, and mixing the mixture to prepare a colloid product.

[0060] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 2MPa, the jelly is stirred at a temperature of 35-40°C, and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40°C for 6 minutes, and finally 15 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0061] Example 5

[0062] This embodiment prepares a Chinese soft-shelled turtle feed. Compared with Example 1, in the process of preparing the fermentation product of water boat stink bug and probiotic Bacillus, thiol protease is also added. The preparation method is as follows:

[0063] 25 parts of water boatmen are taken by weight, ground, 0.8 parts of thiol protease, 0.1 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of water boatmen and probiotic bacillus.

[0064] Take 35 parts of katydid by mass, grind the katydid, add 0.1 parts of bifidobacterium and 15 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0065] The invention discloses a method for preparing a colloid product. The method comprises mixing 41 parts of cod meal, 20 parts of defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.2 parts of lactic acid bacteria, 7 parts of fermentation products of water boatmen and probiotic bacillus, and 7 parts of fermentation products of cicada and bifidobacterium, and then grinding the mixture and passing the mixture through a 60-mesh sieve. The powder particles passing through the sieve are added into 50 parts of water and mixed evenly to prepare a colloid product.

[0066] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0067] Example 6

[0068] This embodiment prepares a Chinese soft-shell turtle feed. Compared with Example 1, the raw materials of the Chinese soft-shell turtle feed also include 0.8 parts of taurine. The preparation method is as follows:

[0069] 25 parts of water boatmen are taken by weight, ground, 0.1 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of the water boatmen and the probiotic bacillus.

[0070] Take 35 parts of katydid by mass, grind the katydid, add 0.1 parts of bifidobacterium and 15 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0071] The invention discloses a method for preparing a colloid product. The method comprises mixing 41 parts of cod meal, 20 parts of defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.2 parts of lactic acid bacteria, 0.8 parts of taurine, 7 parts of fermentation products of water boatmen and probiotic bacillus, and 7 parts of fermentation products of cicada and bifidobacterium, and then pulverizing the mixture, passing through a 60-mesh sieve, adding 50 parts of water to the powder particles passing through the sieve, and mixing the mixture to prepare a colloid product.

[0072] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0073] Example 7

[0074] This embodiment prepares a Chinese soft-shelled turtle feed. Compared with Example 1, 4 parts of squid liver powder, 0.35 parts of choline chloride and 2 parts of motherwort slices are added to the Chinese soft-shelled turtle feed. The preparation method is as follows:

[0075] 25 parts of water boatmen are taken by weight, ground, 0.1 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of the water boatmen and the probiotic bacillus.

[0076] Take 35 parts of katydid by mass, grind the katydid, add 0.1 parts of bifidobacterium and 15 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0077] By weight, 41 parts of cod meal, 20 parts of fully defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.2 parts of lactic acid bacteria, 4 parts of squid liver powder, 0.35 parts of choline chloride, 2 parts of motherwort slices, 7 parts of fermentation products of water boat stink bugs and probiotic Bacillus, and 7 parts of fermentation products of knitting bugs and bifidobacteria were mixed, crushed, passed through a 60-mesh sieve, and 50 parts of the powder particles passing through the sieve were added.

[0078] Mix with water to form a gel.

[0079] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0080] Example 8

[0081] This embodiment prepares a Chinese soft-shelled turtle feed. Compared with Example 1, in the process of preparing the fermentation product of water boat stink bug and probiotic bacillus, thiol protease is also added. 0.8 parts of taurine, 4 parts of squid liver powder, 0.35 parts of choline chloride and 2 parts of motherwort slices are also added to the Chinese soft-shelled turtle feed. The preparation method is as follows:

[0082] 25 parts of water boatmen are taken by weight, ground, 0.8 parts of thiol protease, 0.1 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of water boatmen and probiotic bacillus.

[0083] Take 35 parts of katydid by mass, grind the katydid, add 0.1 parts of bifidobacterium and 15 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0084] The invention discloses a method for preparing a colloid product. The method comprises mixing 41 parts of cod meal, 20 parts of defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.2 parts of lactic acid bacteria, 0.8 parts of taurine, 4 parts of squid liver powder, 0.35 parts of choline chloride, 2 parts of motherwort slices, 7 parts of fermentation products of water boatmen and probiotic bacillus, and 7 parts of fermentation products of cicada and bifidobacterium, and then crushing the mixture, passing through a 60-mesh sieve, adding 50 parts of water to the powder particles passing through the sieve, and mixing the mixture to prepare a colloid product.

[0085] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0086] Comparative Example 1

[0087] Compared with Example 1, the present comparative example 1 does not add the fermentation product of water boat stink bug and probiotic Bacillus to prepare the Chinese soft-shelled turtle feed. The method for preparing the Chinese soft-shelled turtle feed in the present comparative example 1 is as follows.

[0088] Take 35 parts of katydid by mass, grind the katydid, add 0.1 parts of bifidobacterium and 15 parts of water, mix well and seal, ferment for 8 hours at 36-40°C while maintaining pH at 6.5-7.0 to obtain a fermentation product of katydid and bifidobacterium.

[0089] The invention discloses a method for preparing a colloid product by mixing 41 parts of cod meal, 20 parts of fully defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of enveloped vitamin C, 0.2 parts of lactic acid bacteria and 7 parts of fermentation products of cicada and bifidobacterium, and then grinding the mixture and passing the mixture through a 60-mesh sieve. The powder particles passing through the sieve are added with 50 parts of water and mixed evenly to prepare a colloid product.

[0090] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0091] Comparative Example 2

[0092] Compared with Example 1, the fermentation product of cicada and bifidobacterium was not added in the preparation of Chinese soft-shelled turtle feed in Comparative Example 2. The method for preparing Chinese soft-shelled turtle feed in Comparative Example 2 is as follows.

[0093] 25 parts of water boatmen are taken by weight, ground, 0.1 parts of probiotic bacillus and 10 parts of water are added, mixed, and fermented for 1 day at 35-38° C. while maintaining the pH at 6.5-7.5 to obtain a fermentation product of the water boatmen and the probiotic bacillus.

[0094] The invention discloses a method for preparing a colloid product by mixing 41 parts of cod meal, 20 parts of fully defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of coated vitamin C, 0.2 parts of lactic acid bacteria and 7 parts of fermentation products of water boatmen and probiotic bacillus, and then grinding the mixture and passing the mixture through a 60-mesh sieve. The powder particles passing through the sieve are added into 50 parts of water and mixed evenly to prepare a colloid product.

[0095] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0096] Comparative Example 3

[0097] Compared with Example 1, in this comparative example 3, the fermentation product of water boatman and probiotic Bacillus, and the fermentation product of cicada and Bifidobacterium are not added in the preparation of Chinese soft-shelled turtle feed. The method for preparing Chinese soft-shelled turtle feed in this comparative example 3 is as follows.

[0098] According to the weight percentage, 41 parts of cod meal, 20 parts of fully defatted anchovy meal, 19 parts of black soldier fly meal, 21 parts of pregelatinized starch, 5 parts of enzymatically hydrolyzed soybean meal, 2 parts of minerals, 0.3 parts of multivitamins, 0.1 parts of coated vitamin C and 0.2 parts of lactic acid bacteria are mixed, and then crushed and passed through a 60-mesh sieve, and 50 parts of water are added to the powder particles passing through the sieve and mixed to form a colloid.

[0099] The jelly is placed in an extruder, the air pressure in the extruder is adjusted to 1.5 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 5 minutes, and finally 10 parts of emulsified fish oil are evenly covered on the surface of all the expanded particles after the insulation to obtain Chinese soft-shelled turtle feed.

[0100] Test Example 1

[0101] The same batch of Chinese soft-shelled turtles with basically the same growth and development conditions were divided into 11 groups, with 100 in each group, and fed with the feed prepared by Examples 1-8 and Comparative Examples 1-3 for 2 months, respectively. The same quality of feed was fed every day using the same method to verify the growth and development differences between different groups after 2 months of feeding.

[0102] Diseases of Chinese soft-shelled turtles include Saprolegniasis, mumps, enteritis, plastron hemorrhage, and skin rot. The incidence rate = total number of symptoms in each group of Chinese soft-shelled turtles / total number of Chinese soft-shelled turtles in each group.

[0103] The test results are shown in Table 1.

[0104] Table 1 Growth and development of Chinese soft-shell turtle after feeding the feeds of each embodiment and comparative example

[0105]

[0106]

[0107]

[0108] As can be seen from Table 1, the feed prepared in Examples 1-8 has a good promoting effect on the growth and development of Chinese soft-shelled turtles, and its incidence rate is low and the edible taste is good. The growth and development effects of Examples 1-2 and Examples 3-4 are better. Example 5 Compared with Example 1, in the process of preparing the fermentation product of water boat stink bug and probiotic Bacillus, a thiol protease is also added, and its growth and development effect is better. This is because after adding the thiol protease, the fermentation product of water boat stink bug and probiotic Bacillus can better promote the growth and development of Chinese soft-shelled turtles. Example 6 Compared with Example 1, the raw materials of Chinese soft-shelled turtle feed also include 0.8 parts of taurine, and its incidence rate is also reduced. This is because taurine is added to the feed, and the feed can promote the improvement of the physique of Chinese soft-shelled turtles. Example 7 Compared with Example 1, 4 parts of squid liver powder, 0.35 parts of choline chloride and 2 parts of mother-feeding slices were added to the Chinese soft-shelled turtle feed, and the growth and development of the Chinese soft-shelled turtle was better. This is because the squid liver powder contains a variety of nutrients, which has the effects of protecting the eyes, nourishing blood and calming the nerves; choline chloride can reduce the risk of fatty liver and cirrhosis; mother-feeding slices can boost appetite and improve indigestion. The three cooperate to promote the growth and development of the Chinese soft-shelled turtle. Example 8 Compared with Example 1, in the process of preparing the fermentation product of water boat stink bug and probiotic Bacillus, thiol protease was also added, and 0.8 parts of taurine, 4 parts of squid liver powder, 0.35 parts of choline chloride and 2 parts of mother-feeding slices were also added to the Chinese soft-shelled turtle feed. The growth and development of the Chinese soft-shelled turtle was better, and its incidence rate was also reduced. This is a manifestation of the shared effect of the above ingredients.

[0109] Relatively speaking, the feed prepared in Comparative Examples 1-3 has a better promoting effect on the growth and development of Chinese soft-shelled turtles, but the growth and development is not as good as the effect obtained by the feed prepared in Examples 1-8. The incidence of Chinese soft-shelled turtles was higher when the feed prepared in Comparative Examples 1-3 was fed, which may be because the feed had a limited effect on improving the incidence of Chinese soft-shelled turtles, and the Chinese soft-shelled turtles had a slightly worse taste when fed with the feed.

[0110] The Chinese soft-shelled turtle feed prepared in Examples 1-8 improves the physical fitness and immunity of the Chinese soft-shelled turtle and increases the growth rate of the Chinese soft-shelled turtle.

[0111] The above is only a preferred embodiment of the present application, and the protection scope of the present application is not limited to the above embodiment. All technical solutions under the concept of the present application belong to the protection scope of the present application. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present application should also be regarded as falling within the protection scope of the present application.

Claims

1. A Chinese soft-shelled turtle feed, characterized in that: The Chinese soft-shelled turtle feed is made of 39-44 parts of cod meal, 16-25 parts of anchovy meal, 18-22 parts of black soldier fly meal, 20-23 parts of starch, 3-8 parts of soybean meal, 1-4 parts of minerals, 0.2-0.5 parts of multivitamins, 0.1-0.3 parts of enveloped vitamin C, 5-15 parts of emulsified fish oil, 0.1-0.4 parts of lactic acid bacteria, 5-10 parts of fermentation products of water boat stink bugs and probiotic bacillus, and 5-10 parts of fermentation products of knitting bugs and bifidobacteria by mass; Grind the water boatman, add probiotic Bacillus and water, mix well, and ferment for 1 to 2 days at 35 to 38° C. while maintaining the pH at 6.5 to 7.5 to obtain a fermentation product of the water boatman and the probiotic Bacillus; The katydid was ground, bifidobacteria and water were added, the mixture was mixed and sealed, and fermented for 8 to 16 hours at 36 to 40° C. while maintaining the pH at 6.5 to 7.0 to obtain a fermentation product of the katydid and bifidobacteria.

2. The Chinese soft-shelled turtle feed according to claim 1, characterized in that: The Chinese soft-shelled turtle feed is made of 41 to 42 parts of cod meal, 20 to 21 parts of anchovy meal, 19 to 20 parts of black soldier fly meal, 21 to 22 parts of starch, 5 to 6 parts of soybean meal, 2 to 3 parts of minerals, 0.3 to 0.4 parts of multivitamins, 0.1 to 0.2 parts of enveloped vitamin C, 9 to 11 parts of emulsified fish oil, 0.2 to 0.3 parts of lactic acid bacteria, 7 to 8 parts of fermentation products of water boatmen and probiotic bacillus, and 7 to 8 parts of fermentation products of katydids and bifidobacteria, by mass.

3. The Chinese soft-shelled turtle feed according to claim 1 or 2, characterized in that: The multivitamin includes vitamin A, vitamin D, vitamin E, vitamin K3, vitamin B 12 , vitamin B6, vitamin B2, vitamin B1, niacin, folic acid and pantothenic acid.

4. The Chinese soft-shelled turtle feed according to claim 1 or 2, characterized in that: The raw materials of the emulsified fish oil include anchovy oil, krill oil, an emulsifier and water.

5. The Chinese soft-shelled turtle feed according to claim 1 or 2, characterized in that: The Chinese soft-shell turtle feed is further added with 0.5 to 1 portion of taurine.

6. The Chinese soft-shelled turtle feed according to claim 1 or 2, characterized in that: The Chinese soft-shelled turtle feed is further added with 2-6 parts of squid liver powder, 0.2-0.5 parts of choline chloride and 1-3 parts of motherwort slices.

7. A method for preparing a Chinese soft-shelled turtle feed according to any one of claims 1 to 6, characterized in that: The preparation method comprises: Mixing, crushing, and sieving the other ingredients except the emulsified fish oil, adding water and mixing well to form a jelly; The jelly is placed in the equipment, the air pressure in the equipment is adjusted to 1-2 MPa, the jelly is stirred at a temperature of 35-40° C., and then extruded into the atmosphere to obtain expanded particles, the expanded particles are kept warm at 35-40° C. for 4-6 minutes, and finally the surface of the expanded particles after insulation is covered with the emulsified fish oil to obtain the Chinese soft-shelled turtle feed.

8. The method for preparing Chinese soft-shelled turtle feed according to claim 7, characterized in that: The soybean meal is enzymatically hydrolyzed soybean meal, which is obtained by adding endo-enzyme, terminal peptidase and flavor enzyme into the soybean meal to decompose the soybean meal and hydrolyze the soybean protein into peptone, polypeptide and amino acid.

9. The method for preparing Chinese soft-shelled turtle feed according to claim 7, characterized in that: In the process of preparing the fermentation product of water boatman and probiotic bacillus, thiol protease is also added.

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