A cell nutrition supplement and preparation method thereof

Abstract

The present invention proposes a cell nutrition supplement and a preparation method thereof, and belongs to the technical field of nutritional food. Snail meat is enzymatically hydrolyzed to obtain snail protein peptides, which are mixed with composite protein hydrolysates obtained from kudzu vine, ginseng and tau kudzu fruit powder, chelated with chromium and zinc, and protein peptide chelates are obtained; kudzu vine, ginseng and tau kudzu fruit powder are also used to prepare composite polysaccharides and antioxidant compositions, solid residues are added to water, fermented by selenium-enriched yeast and Bacillus licheniformis, the products are embedded by composite polysaccharides and sodium caseinate, and are homogenized with β-cyclodextrin, arabinose, antioxidant compositions, protein peptide chelates, lecithin and palm kernel oil under high pressure, microfluidization, freeze-dried and sterilized to obtain a nutritional supplement with a high protein ratio, which improves immunity, regulates glucose metabolism, improves insulin secretion and reduces insulin resistance, and has broad application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional foods, and in particular to a cell nutritional supplement and a preparation method thereof. Background Art

[0002] Formulated foods (Foods for Special Medical Purpose, FSMP), in short, nutritious foods, are a type of formula foods that are specially processed and prepared to meet the physiological and nutritional needs of specific groups of people or patients. They need to be used under the guidance of a clinician or nutritionist.

[0003] The Guidelines for Perioperative Nutritional Support in Adults compiled by the Parenteral and Enteral Nutrition Branch of the Chinese Medical Association states that the protein supply should be increased to 2.0 g kg -1 d -1 It can achieve a more ideal treatment effect, especially for patients with major surgical trauma, who have even higher protein requirements. When patients are in a stress period, the body breaks down more protein, the amount of acute phase protein synthesis increases, and the demand for essential amino acids increases rapidly. At this time, increasing protein intake can increase the synthesis of muscle protein, liver acute phase protein, and immune system protein, reduce the amount of protein lost by patients, improve potential and existing malnutrition, and prevent and treat complications. Give patients high-protein (≥1.2 g kg) within 3 days after admission to the hospital for critically ill patients. -1 d -1 ) Nutritional support can significantly improve the patient's muscle strength and have a positive effect on the patient's later recovery. High-protein nutritional support can effectively correct the negative nitrogen balance of critically ill patients during their stay in the ICU and improve their nutritional status. However, patients with congestive heart failure, pulmonary failure, etc. may suffer from volume overload and congestion, leading to lung infection, myocardial ischemia, decreased contractility, abnormal liver function, renal insufficiency and many other problems. Excessive fluid intake can increase the circulatory burden. Therefore, the "Chinese Expert Recommendations on Volume Management for Heart Failure" of the Heart Failure Professional Committee of the Chinese Medical Association advocates that the amount of fluid for adults should be 30 mg / kg -1 , including dietary intake and fluid infusion. In addition, some patients have symptoms of edema, such as hypoproteinemia caused by kidney or liver disease, venous insufficiency, thyroid dysfunction, antidiuretic hormone secretion disorder syndrome and glucocorticoid deficiency. For the above patients, increasing protein intake helps to keep water in the blood vessels and prevent body fluids from leaking into the tissues.

[0004] Therefore, designing and preparing a high-protein, complete nutrition formula food product has a very huge market prospect. Summary of the invention

[0005] The purpose of the present invention is to provide a cell nutrition supplement and a preparation method thereof, which has the advantages of excellent sensory organoleptic properties, uniform stability, high physiological activity, easier digestion, absorption and metabolism by the human body, high protein ratio, improved immunity, and regulation of glucose metabolism, and has broad application prospects.

[0006] The technical solution of the present invention is achieved in this way:

[0007] The invention provides a preparation method of a cell nutrition supplement. The snail meat is enzymatically hydrolyzed to obtain snail protein peptides, which are mixed with a composite protein hydrolysate prepared from smilax glabra, ginseng and taurantii powder, and chelated with chromium and zinc to obtain a protein peptide chelate. The smilax glabra, ginseng and taurantii powder are also used to prepare a composite polysaccharide and an antioxidant composition. Solid residues are added into water, fermented with selenium-enriched yeast and bacillus licheniformis, the product is embedded with the composite polysaccharide and sodium caseinate, and is homogenized with beta-cyclodextrin, arabinose, the antioxidant composition, the protein peptide chelate, lecithin and palm kernel oil under high pressure, subjected to microfluidization treatment, freeze-dried and sterilized to obtain a cell nutrition supplement.

[0008] As a further improvement of the present invention, the following steps are included:

[0009] S1. Add snail meat to water, mince, add trypsin, perform the first enzymatic hydrolysis, filter, retain the solid, add composite protease to the filtrate, perform the second enzymatic hydrolysis, dialyze, freeze-dry, and obtain snail protein peptide;

[0010] S2. The powder of Gexianmi algae is added to water to swell, frozen with liquid nitrogen, rewarmed at room temperature, and Baole fruit powder and ginseng powder are added, stirred and mixed evenly, snail enzyme is added to hydrolyze and break the wall, filtered, the solid is retained, the filtrate is subjected to ammonium sulfate graded precipitation, filtered, washed, and freeze-dried to obtain a composite protein, the filtrate is added with ethanol precipitation, filtered, washed, and dried to obtain a composite polysaccharide, the filtrate is recovered with ethanol, dialyzed to remove salt, and the liquid is dried to obtain an antioxidant composition;

[0011] S3. adding the complex protein to water, adding protease to hydrolyze, and obtaining a complex protein peptide;

[0012] S4. The composite protein peptide and snail protein peptide were mixed and added to water, chromium salt and zinc salt were added, stirred for reaction, dialyzed, and freeze-dried to obtain a protein peptide chelate;

[0013] S5. The solid in step S1 and the solid in step S2 are mixed and added to water, inoculated with selenium-enriched yeast and Bacillus licheniformis, fermented and cultured, the product is filtered, the bacterial solution is washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex;

[0014] S6. The complex polysaccharide and sodium caseinate were dissolved in water, the fermentation - bacteria complex and lecithin were added, the mixture was stirred and evenly mixed, the mixture was dropped into the fish oil, emulsified, and freeze-dried to obtain encapsulated microspheres;

[0015] S7. Add β-cyclodextrin, arabinose, antioxidant composition, protein peptide chelate, embedded microspheres, and lecithin into water, add palm kernel oil, high pressure homogenize, microfluidize to obtain an emulsion, freeze dry, and sterilize to obtain a cell nutrition supplement.

[0016] As a further improvement of the present invention, the mass ratio of snail meat, trypsin and composite protease in step S1 is 10:0.5-1:1-2, the temperature of the first enzymolysis is 36-38°C, the time is 1-2h, the temperature of the second enzymolysis is 40-50°C, the time is 2-4h, and the composite protease is selected from at least two of neutral protease, ficin, bromelain and papain, preferably, it is a mixture of neutral protease and ficin, and the mass ratio is 4-7:2.

[0017] As a further improvement of the present invention, the solid-liquid ratio of the kudzu vine powder and water in step S2 is 1:7-10 g / mL, the mass ratio of the kudzu vine powder, tauropana powder, ginseng powder and snail enzyme is 10-20:7-10:5-7:1-2, the enzymatic hydrolysis temperature is 40-50°C, and the time is 1-3h.

[0018] Preferably, the helicase is replaced by immobilized helicase, and the preparation method of immobilized helicase is as follows:

[0019] Soaking the sodium type molecular sieve in an alkaline solution, filtering, washing, and drying to obtain a pretreated molecular sieve;

[0020] The pretreated molecular sieve is added into water, and under the protection of inert gas, ferric chloride and ferrous chloride are added, and ammonia water is added dropwise, and the reaction is heated and stirred, centrifuged, washed, dried, and calcined to obtain a magnetic molecular sieve;

[0021] The modified magnetic molecular sieve is prepared by adding the magnetic molecular sieve into water, adding dopamine hydrochloride and a catalyst, heating and stirring to react, separating by magnet, washing and drying.

[0022] The snail enzyme is dissolved in water, and the modified magnetic molecular sieve is added, the mixture is stirred and mixed evenly, and the solvent is evaporated to obtain the immobilized snail enzyme.

[0023] As a further improvement of the present invention, the mass ratio of the composite protein to the protease in step S3 is 15-20:1-2, the protease is selected from at least one of neutral protease, ficin, bromelain, papain, alkaline protease, trypsin, and pepsin, preferably, a mixture of bromelain and papain, with a mass ratio of 2-4:3, the temperature of the enzymolysis is 35-50° C., and the time is 30-60 min.

[0024] As a further improvement of the present invention, in step S4, the mass ratio of the composite protein peptide, snail protein peptide, chromium salt and zinc salt is 10-20:5-10:0.5-1:0.2-0.5, and the stirring reaction time is 20-40 min.

[0025] As a further improvement of the present invention, in step S5, the mass ratio of the solid in step S1 to the solid in step S2 is 5:10-12, the inoculation amounts of the selenium-enriched yeast and Bacillus licheniformis are 2-3v / v% and 1-2v / v%, respectively, and the fermentation culture conditions are 40-45°C, 100-200r / min, and the fermentation culture is 24-48h.

[0026] As a further improvement of the present invention, the mass ratio of the complex polysaccharide, sodium caseinate, fermentation-bacteria complex and lecithin in step S6 is 4-6:7-10:5-7:0.1-0.2.

[0027] As a further improvement of the present invention, the mass ratio of β-cyclodextrin, arabinose, antioxidant composition, protein peptide chelate, embedded microspheres, lecithin, and palm kernel oil in step S7 is 5-7:4-6:1-2:2-3:2-4:0.1-0.2:15-20, the pressure of the high-pressure homogenization is 400-500 Bar, the time is 10-20 min, and the pressure of the microjet treatment is 90-110 MPa.

[0028] The present invention further protects a cell nutrition supplement prepared by the above preparation method.

[0029] The present invention has the following beneficial effects:

[0030] Currently, the protein sources of complete nutritional food products on the market are mostly casein and its salts or milk protein with casein as the main component. Their special micellar structure, such as the nanoclusters formed by the combination of phosphorylated group clusters with calcium, sodium and other ions, can further stabilize the protein structure, and have adverse effects such as reducing the frequency and amplitude of intestinal contractions and inhibiting lymphocyte proliferation. In addition, due to the slow digestion and absorption kinetics after ingestion and low plasma amino acid utilization, they are "slowly digesting proteins". Compared with "fast digesting proteins" such as plant-based proteins or marine protein peptides, plant-based proteins or marine protein peptides can increase amino acid levels and increase muscle content more quickly, which is more beneficial to improving patients' protein malnutrition. They also have the effects of lowering blood lipids and regulating intestinal microorganisms.

[0031] The plant protein peptides used in the present invention are kudzu vine and ginseng protein. Kudzu vine is a freshwater algae, rich in vitamin C, protein and amino acids (dry matter content exceeds 30%), polysaccharides (dry matter content exceeds 30%) and other active components, but its cell wall is thick, and it is difficult to use ordinary wall breaking methods to break the wall, so that the active substances are dissolved. The present invention adopts a droplet freezing-rewarming method, and the contents form an "ice knife" to pierce the cell wall. At the same time, a snail enzyme enzymolysis method is used to destroy the cell wall and enzymolyze, so that most of the active components can be dissolved.

[0032] Ginseng is rich in ginsenosides, ginseng polysaccharides and ginseng protein. Baole fruit is also rich in antioxidants such as flavonoids, vitamin C, vitamin E, carotene, etc., and also rich in polysaccharides.

[0033] The invention discloses an antioxidant composition comprising a composite polysaccharide and a composite protein prepared from kudzu vine, ginseng and bao le fruit powder, and rich antioxidant substances such as vitamin C, vitamin E, saponin, flavonoids, phenols and carotene.

[0034] The small molecule oligopeptides obtained by enzymatic hydrolysis of the complex protein are easily digested and absorbed, and mixed with snail protein peptides to obtain bioactive peptides. The bioactive peptides can inhibit dipeptidyl peptidase 4 inhibitors and key enzymes related to carbohydrate metabolism, including α-amylase and α-glucosidase.

[0035] Bioactive peptides chelate chromium and zinc ions. Chromium is an important component of glucose tolerance factor, which can participate in the body's sugar metabolism, activate insulin to lower blood sugar, and maintain normal sugar metabolism in the body. Zinc participates in a variety of enzyme reactions in the human body and is an important trace element in the human body. It plays an important role in improving human immunity. However, direct intake of zinc and chromium is usually not highly absorbed in the human body. The zinc and chromium formed by protein peptide chelation can directly absorb protein through the small intestine while absorbing trace elements, greatly improving the absorption and utilization rate of trace elements, thereby better playing the role of improving immunity.

[0036] The solid residue after extraction still contains rich nutrients. Fermentation with selenium-rich yeast and Bacillus licheniformis produces rich nutritious fermentation byproducts, including short-chain fatty acids such as L-lactic acid. L-lactic acid can participate in biosynthesis as an energy carrier, and can also be used as a signal molecule to regulate the release of hormones and the activity of various enzymes in the body. It has the effects of promoting intestinal digestion and absorption, improving insulin resistance, protecting intestinal probiotics, reducing blood lipids and preventing atherosclerosis. Or it can produce selenium-rich products. Selenium can reduce hyperglycemia or enhance insulin sensitivity to a certain extent; on the other hand, it promotes the proliferation of fermentation bacteria, thereby obtaining fermentation bacteria with high activity and rich content. Through the encapsulation technology, the fermentation product is encapsulated with the prepared composite polysaccharide as the shell material, which improves the survival rate and stability of probiotics on the one hand, and on the other hand, the composite polysaccharide also has the effect of promoting the growth of probiotics and improving the body's antioxidant and immune functions.

[0037] Palm kernel oil is added during the preparation of the emulsion of the present invention. The palm kernel oil is extracted by pressing and is rich in medium-chain lauric acid and myristic acid. It is a natural food source of medium-chain triglycerides, has the advantages of small molecular weight, fast hydrolysis rate, and is easier to be digested, absorbed and metabolized by the human body. It has stable chemical properties, is not prone to peroxidation, is rich in fatty acids, can directly act on lipase, and make the arterial blood vessel wall smooth, smooth and elastic.

[0038] High-pressure homogenization and microfluidization technology use collision, shearing, cavitation blasting and other physical forces to make the material reach the micron level or even the nano level. The prepared emulsion has the characteristics of uniformity and stability, high preparation efficiency, low dependence on additives, and excellent sensory properties. Freeze drying is further adopted to solve the problem of uneven mixing between the nutritional components. At the same time, it also avoids the inactivation of active proteins and live bacteria during the high temperature process of spray drying, thereby ensuring the physiological activity of the obtained product.

[0039] The cell nutrition supplement prepared by the present invention has the advantages of excellent sensory organoleptic properties, uniform stability, high physiological activity, easier digestion, absorption and metabolism by the human body, high protein ratio, improved immunity, and regulation of glucose metabolism, and has broad application prospects. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Snail enzyme, with a cell wall breaking rate of 95%, was purchased from Zhangzhou Yeshi Biotechnology Co., Ltd.; trypsin, 250,000 U / g, neutral protease, 60,000 U / g, bromelain, 100,000 U / g, papain, 100,000 U / g, were purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.; fig protease, 50,000 U / g, was purchased from Shandong Pingju Biotechnology Co., Ltd.

[0042] Sodium molecular sieve, 4A, spherical, 3-5 mm, purchased from Henan Yujia Environmental Protection Materials Co., Ltd.

[0043] Selenium-enriched yeast, 10 billion cfu / g, was purchased from Angel Yeast Co., Ltd. Bacillus licheniformis, 20 billion cfu / g, was purchased from Hebei Hongtao Bioengineering Co., Ltd.

[0044] The preparation method of selenium-enriched yeast and Bacillus licheniformis seed solution is as follows: Selenium-enriched yeast and Bacillus licheniformis were inoculated into Gao's medium respectively, and activated and cultured for 24 hours at 40°C and 100r / min to obtain a culture medium with a bacterial content of 10 8 -10 9 cfu / mL of bacterial seed solution.

[0045] Gexianmi algae powder; Baole fruit powder; Ginseng powder;

[0046] Preparation Example 1 Preparation of immobilized snail enzyme

[0047] Here’s how:

[0048] (1) Soak 10 g of sodium molecular sieve in 100 mL of 10 wt% NaOH solution for 30 min, filter, wash, and dry to obtain a pretreated molecular sieve;

[0049] (2) Add 7 g of pretreated molecular sieve to 100 mL of water, add 1.62 g of ferric chloride, 0.83 g of ferrous chloride and 0.2 g of zinc chloride under nitrogen protection, add 10 mL of 25 wt% ammonia water dropwise, heat to 80 ° C, stir to react for 4 h, centrifuge, wash, dry, and calcine to obtain a magnetic molecular sieve;

[0050] (3) Add 5 g of magnetic molecular sieve to 100 mL of water, add 4 g of dopamine hydrochloride and 2 mL of catalyst, heat to 50 ° C, stir and react for 2 h, separate with a magnet, wash, and dry to obtain a modified magnetic molecular sieve;

[0051] The catalyst is a Tris-HCl solution with a pH of 9.5;

[0052] (4) Dissolve 1 g of snail enzyme in 100 mL of water, add 5 g of modified magnetic molecular sieve, stir and mix evenly, evaporate the solvent, and obtain immobilized snail enzyme.

[0053] Example 1

[0054] This embodiment provides a method for preparing a cell nutrition supplement, comprising the following steps:

[0055] S1. Add 10g snail meat to 300mL water, mince, add 0.5g trypsin, enzymatically hydrolyze at 36℃ for 1h, filter, retain the solid, add 1g composite protease to the filtrate, enzymatically hydrolyze at 40℃ for 2h, dialyze, and freeze-dry to obtain snail protein peptide;

[0056] The composite protease is a mixture of neutral protease and ficin, with a mass ratio of 4:2;

[0057] S2. 10g of Gexianmi algae powder was added to 70mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 7g of Baole fruit powder and 5g of ginseng powder were added, and the mixture was stirred for 20min. 1g of snail enzyme was added, and the cell wall was broken by enzymolysis at 40°C for 1h. The solid was retained, and the filtrate was subjected to ammonium sulfate graded precipitation, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, and the salt was removed by dialysis. The liquid was dried to obtain an antioxidant composition.

[0058] S3. Add 3.75 g of the complex protein to 100 mL of water, add 0.25 g of protease, and perform enzymatic hydrolysis at 35°C for 30 min to obtain a complex protein peptide;

[0059] The protease is a mixture of bromelain and papain in a mass ratio of 2:3;

[0060] S4. 2.5 g of the composite protein peptide and 1.75 g of the snail protein peptide were mixed and added to water, 125 mg of chromium chloride and 50 mg of zinc chloride were added, the reaction was stirred for 20 min, dialyzed, and freeze-dried to obtain a protein peptide chelate;

[0061] S5. 5 g of the solid in step S1 and 10 g of the solid in step S2 were mixed and added to 500 mL of water, and seed solutions of selenium-enriched yeast and Bacillus licheniformis were inoculated at inoculation amounts of 2 v / v% and 1 v / v%, respectively, at 40°C and 100 r / min, and fermented for 24 h. The product was filtered, the bacterial solution was washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex;

[0062] S6. 4 g of complex polysaccharide and 7 g of sodium caseinate were added to 200 mL of water, 5 g of fermentation-bacteria complex and 0.1 g of lecithin were added, stirred for 30 min, dropped into 400 mL of fish oil, emulsified at 10000 r / min for 15 min, and freeze-dried to obtain embedded microspheres;

[0063] S7. Add 5 g of β-cyclodextrin, 4 g of arabinose, 1 g of antioxidant composition, 2 g of protein peptide chelate, 2 g of embedded microspheres, and 0.1 g of lecithin to 200 mL of water, add 15 g of palm kernel oil, homogenize at 400 Bar high pressure for 10 min, and perform microfluidization at 90 MPa to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0064] Example 2

[0065] This embodiment provides a method for preparing a cell nutrition supplement, comprising the following steps:

[0066] S1. Add 10g snail meat to 300mL water, mince, add 1g trypsin, enzymatically hydrolyze at 38℃ for 2h, filter, retain the solid, add 2g composite protease to the filtrate, enzymatically hydrolyze at 50℃ for 4h, dialyze, and freeze-dry to obtain snail protein peptide;

[0067] The composite protease is a mixture of neutral protease and ficin, with a mass ratio of 7:2;

[0068] S2. 20 g of Gexianmi algae powder was added to 200 mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 10 g of Baole fruit powder and 7 g of ginseng powder were added, and the mixture was stirred for 20 min. 2 g of snail enzyme was added, and the cell wall was broken by enzymolysis at 50 ° C for 3 h. The solid was retained, and the filtrate was subjected to ammonium sulfate graded precipitation, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80 wt%, precipitated for 1 h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, and the salt was removed by dialysis. The liquid was dried to obtain an antioxidant composition;

[0069] S3. Add 5 g of the complex protein to 100 mL of water, add 0.5 g of protease, and perform enzymatic hydrolysis at 50°C for 60 min to obtain a complex protein peptide;

[0070] The protease is a mixture of bromelain and papain, with a mass ratio of 4:3;

[0071] S4. 5 g of the composite protein peptide and 2.5 g of the snail protein peptide were mixed and added to water, 250 mg of chromium chloride and 125 mg of zinc chloride were added, the reaction was stirred for 30 min, dialyzed, and freeze-dried to obtain a protein peptide chelate;

[0072] S5. 5 g of the solid in step S1 and 12 g of the solid in step S2 were mixed and added to 500 mL of water, and seed solutions of selenium-enriched yeast and Bacillus licheniformis were inoculated at inoculation amounts of 3 v / v% and 2 v / v%, respectively, at 45°C and 200 r / min, and fermented for 48 h. The product was filtered, the bacterial solution was washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex;

[0073] S6. 6 g of complex polysaccharide and 10 g of sodium caseinate were added to 200 mL of water, 7 g of fermentation-bacteria complex and 0.2 g of lecithin were added, stirred for 30 min, dropped into 400 mL of fish oil, emulsified at 10,000 r / min for 15 min, and freeze-dried to obtain embedded microspheres;

[0074] S7. Add 7 g of β-cyclodextrin, 6 g of arabinose, 2 g of antioxidant composition, 3 g of protein peptide chelate, 4 g of embedded microspheres, and 0.2 g of lecithin to 200 mL of water, add 20 g of palm kernel oil, homogenize at 500 Bar high pressure for 20 min, and perform microfluidization at 110 MPa to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0075] Example 3

[0076] This embodiment provides a method for preparing a cell nutrition supplement, comprising the following steps:

[0077] S1. Add 10g snail meat to 300mL water, mince, add 0.7g trypsin, enzymatically hydrolyze at 37℃ for 1.5h, filter, retain the solid, add 1.5g composite protease to the filtrate, enzymatically hydrolyze at 45℃ for 3h, dialyze, and freeze-dry to obtain snail protein peptide;

[0078] The composite protease is a mixture of neutral protease and ficin, with a mass ratio of 5:2;

[0079] S2. 15g of Gexianmi algae powder was added to 150mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 8g of Baole fruit powder and 6g of ginseng powder were added, and the mixture was stirred for 20min. 1.5g of snail enzyme was added, and the cell wall was broken by enzymolysis at 45°C for 2h. The solid was retained, and the filtrate was subjected to ammonium sulfate graded precipitation, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, and the salt was removed by dialysis. The liquid was dried to obtain an antioxidant composition.

[0080] S3. Add 4.5 g of the complex protein to 100 mL of water, add 0.4 g of protease, and perform enzymatic hydrolysis at 40°C for 45 min to obtain a complex protein peptide;

[0081] The protease is a mixture of bromelain and papain, with a mass ratio of 3:3;

[0082] S4. 2.4 g of the composite protein peptide and 2.2 g of the snail protein peptide were mixed and added to water, 200 mg of chromium chloride and 75 mg of zinc chloride were added, the reaction was stirred for 25 min, dialyzed, and freeze-dried to obtain a protein peptide chelate;

[0083] S5. 5 g of the solid in step S1 and 11 g of the solid in step S2 were mixed and added to 500 mL of water, and seed solutions of selenium-enriched yeast and Bacillus licheniformis were inoculated at inoculation amounts of 2.5 v / v% and 1.5 v / v%, respectively, at 42°C and 150 r / min, and fermented for 36 h. The product was filtered, the bacterial solution was washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex;

[0084] S6. 5 g of complex polysaccharide and 8 g of sodium caseinate were added to 200 mL of water, 6 g of fermentation-bacteria complex and 0.15 g of lecithin were added, stirred for 30 min, dropped into 400 mL of fish oil, emulsified at 10000 r / min for 15 min, and freeze-dried to obtain embedded microspheres;

[0085] S7. Add 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, 3 g of embedded microspheres, and 0.15 g of lecithin to 200 mL of water, add 17 g of palm kernel oil, homogenize at 450 Bar high pressure for 15 min, and perform microfluidization at 100 MPa to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0086] Example 4

[0087] Compared with Example 3, the difference is that the helicase is replaced by the immobilized helicase prepared in Preparation Example 1, wherein the quality of the helicase is ensured to be the same.

[0088] Comparative Example 1

[0089] Compared with Example 3, the difference is that trypsin hydrolysis is not performed in step S1.

[0090] The details are as follows:

[0091] S1. Add 10g snail meat to 300mL water, mince, add 1.5g composite protease, enzymatically hydrolyze at 45℃ for 3h, dialyze, and freeze-dry to obtain snail protein peptide;

[0092] The composite protease is a mixture of neutral protease and ficin, with a mass ratio of 5:2.

[0093] Comparative Example 2

[0094] Compared with Example 3, the difference is that no composite protease enzymatic hydrolysis is performed in step S1.

[0095] The details are as follows:

[0096] S1. Add 10g snail meat into 300mL water, mince, add 0.7g trypsin, enzymatically hydrolyze at 37℃ for 1.5h, filter, retain the solid, dialyze the filtrate, and freeze-dry to obtain snail protein peptide.

[0097] Comparative Example 3

[0098] Compared with Example 3, the difference is that no snail enzyme is added for enzymolysis in step S2.

[0099] The details are as follows:

[0100] S2. 15g of Gexianmi algae powder is added to 150mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 8g of Baole fruit powder and 6g of ginseng powder are added, and the mixture is stirred for 20min. The mixture is heated to boiling for 2h, filtered, and the solid is retained. The filtrate is subjected to graded precipitation with ammonium sulfate, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol is added to the filtrate until the ethanol content of the system is 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol is recovered from the filtrate, dialyzed to remove salt, and the liquid is dried to obtain an antioxidant composition.

[0101] Comparative Example 4

[0102] Compared with Example 3, the difference is that no Myriophyllum puerariae powder is added in step S2.

[0103] The details are as follows:

[0104] S2. 8g of tauropana powder and 6g of ginseng powder were stirred and mixed for 20min, 1.5g of snail enzyme was added, and the cell wall was broken by enzymolysis at 45℃ for 2h. The solid was filtered and the solid was retained. The filtrate was graded and precipitated with ammonium sulfate, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, dialyzed to remove salt, and the liquid was dried to obtain an antioxidant composition.

[0105] Comparative Example 5

[0106] Compared with Example 3, the difference is that no ginseng powder is added in step S2.

[0107] The details are as follows:

[0108] S2. 15g of Gexianmi algae powder was added to 150mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 8g of Baole fruit powder was added and stirred for 20min. 1.5g of snail enzyme was added and enzymatically hydrolyzed at 45℃ for 2h to break the wall. The solid was filtered and the solid was retained. The filtrate was subjected to graded precipitation with ammonium sulfate, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, dialyzed to remove salt, and the liquid was dried to obtain an antioxidant composition.

[0109] Comparative Example 6

[0110] Compared with Example 3, the difference is that no bao le powder is added in step S2.

[0111] The details are as follows:

[0112] S2. 15g of Gexianmi algae powder was added to 150mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 6g of ginseng powder was added and stirred for 20min. 1.5g of snail enzyme was added and enzymatically hydrolyzed at 45℃ for 2h to break the wall. The solid was filtered and the solid was retained. The filtrate was subjected to graded precipitation with ammonium sulfate, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, dialyzed to remove salt, and the liquid was dried to obtain an antioxidant composition.

[0113] Comparative Example 7

[0114] Compared with Example 3, the difference is that chromium chloride is not added in step S4.

[0115] The details are as follows:

[0116] S4. 2.4 g of the complex protein peptide and 2.2 g of the snail protein peptide were mixed and added to water, and 275 mg of zinc chloride was added. The mixture was stirred for 25 min, dialyzed, and freeze-dried to obtain a protein peptide chelate.

[0117] Comparative Example 8

[0118] Compared with Example 3, the difference is that zinc chloride is not added in step S4.

[0119] The details are as follows:

[0120] S4. 2.4 g of the complex protein peptide and 2.2 g of the snail protein peptide were mixed and added to water, and 275 mg of chromium chloride was added. The mixture was stirred for 25 min, dialyzed, and freeze-dried to obtain a protein peptide chelate.

[0121] Comparative Example 9

[0122] Compared with Example 3, the difference is that chromium chloride and zinc chloride are not added in step S4.

[0123] The details are as follows:

[0124] S4. 2.4 g of the composite protein peptide and 2.2 g of the snail protein peptide were mixed and freeze-dried to prepare a protein peptide mixture.

[0125] Comparative Example 10

[0126] Compared with Example 3, the difference is that no selenium-enriched yeast seed liquid is inoculated in step S5.

[0127] The details are as follows:

[0128] S5. 5 g of the solid in step S1 and 11 g of the solid in step S2 were mixed and added to 500 mL of water, and seed liquid of Bacillus licheniformis was inoculated at an inoculation rate of 4 v / v%, 42°C, 150 r / min, and fermented for 36 h. The product was filtered, the bacterial liquid was washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex.

[0129] Comparative Example 11

[0130] Compared with Example 3, the difference is that the Bacillus licheniformis seed liquid is not inoculated in step S5.

[0131] The details are as follows:

[0132] S5. Mix 5 g of the solid in step S1 and 11 g of the solid in step S2, add to 500 mL of water, inoculate with selenium-enriched yeast seed liquid at an inoculation rate of 4 v / v%, culture at 42°C, 150 r / min, ferment for 36 h, filter the product, wash and collect the bacterial liquid, mix with the filtrate, and freeze-dry to obtain a fermentation-bacteria complex.

[0133] Comparative Example 12

[0134] Compared with the embodiment 3, the difference is that step S6 is not performed.

[0135] The details are as follows:

[0136] S1. Add 10g snail meat to 300mL water, mince, add 0.7g trypsin, enzymatically hydrolyze at 37℃ for 1.5h, filter, retain the solid, add 1.5g composite protease to the filtrate, enzymatically hydrolyze at 45℃ for 3h, dialyze, and freeze-dry to obtain snail protein peptide;

[0137] The composite protease is a mixture of neutral protease and ficin, with a mass ratio of 5:2;

[0138] S2. 15g of Gexianmi algae powder was added to 150mL of water to swell, frozen with liquid nitrogen, and warmed to room temperature. 8g of Baole fruit powder and 6g of ginseng powder were added, and the mixture was stirred for 20min. 1.5g of snail enzyme was added, and the cell wall was broken by enzymolysis at 45°C for 2h. The solid was retained, and the filtrate was subjected to ammonium sulfate graded precipitation, filtered, washed, and freeze-dried to obtain a composite protein. Ethanol was added to the filtrate until the ethanol content of the system was 80wt%, precipitated for 1h, filtered, washed, and dried to obtain a composite polysaccharide. Ethanol was recovered from the filtrate, and the salt was removed by dialysis. The liquid was dried to obtain an antioxidant composition.

[0139] S3. Add 4.5 g of the complex protein to 100 mL of water, add 0.4 g of protease, and perform enzymatic hydrolysis at 40°C for 45 min to obtain a complex protein peptide;

[0140] The protease is a mixture of bromelain and papain, with a mass ratio of 3:3;

[0141] S4. 2.4 g of the composite protein peptide and 2.2 g of the snail protein peptide were mixed and added to water, 200 mg of chromium chloride and 75 mg of zinc chloride were added, the reaction was stirred for 25 min, dialyzed, and freeze-dried to obtain a protein peptide chelate;

[0142] S5. 5 g of the solid in step S1 and 11 g of the solid in step S2 were mixed and added to 500 mL of water, and seed solutions of selenium-enriched yeast and Bacillus licheniformis were inoculated at inoculation amounts of 2.5 v / v% and 1.5 v / v%, respectively, at 42°C and 150 r / min, and fermented for 36 h. The product was filtered, the bacterial solution was washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex;

[0143] S6. Add 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, 3 g of fermentation-bacteria complex, and 0.15 g of lecithin to 200 mL of water, add 17 g of palm kernel oil, homogenize at 450 Bar high pressure for 15 min, and perform microfluidization at 100 MPa to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0144] Comparative Example 13

[0145] Compared with Example 3, the difference is that no antioxidant composition is added in step S7.

[0146] The details are as follows:

[0147] S7. 6 g of β-cyclodextrin, 5 g of arabinose, 2.5 g of protein peptide chelate, 3 g of embedded microspheres, and 0.15 g of lecithin were added to 200 mL of water, and 17 g of palm kernel oil was added. The mixture was homogenized at 450 Bar for 15 min and subjected to microfluidization at 100 MPa to obtain an emulsion. The emulsion was freeze-dried to obtain a cell nutrition supplement.

[0148] Comparative Example 14

[0149] Compared with Example 3, the difference is that no protein-peptide chelate is added in step S7.

[0150] The details are as follows:

[0151] S7. 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 3 g of embedded microspheres, and 0.15 g of lecithin were added to 200 mL of water, and 17 g of palm kernel oil was added. The mixture was homogenized at 450 Bar for 15 min and subjected to microfluidization at 100 MPa to obtain an emulsion. The emulsion was freeze-dried to obtain a cell nutrition supplement.

[0152] Comparative Example 15

[0153] Compared with Example 3, the difference is that no embedded microspheres are added in step S7.

[0154] The details are as follows:

[0155] S7. 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, and 0.15 g of lecithin were added to 200 mL of water, and 17 g of palm kernel oil was added. The mixture was homogenized at 450 Bar for 15 min and subjected to microfluidization at 100 MPa to obtain an emulsion. The emulsion was freeze-dried to obtain a cell nutrition supplement.

[0156] Comparative Example 16

[0157] Compared with Example 3, the difference is that the homogenization pressure in step S7 is 300 Bar.

[0158] The details are as follows:

[0159] S7. Add 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, 3 g of embedded microspheres, and 0.15 g of lecithin to 200 mL of water, add 17 g of palm kernel oil, homogenize at 300 Bar high pressure for 15 min, and perform microfluidization at 100 MPa to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0160] Comparative Example 17

[0161] Compared with Example 3, the difference is that the homogenization pressure in step S7 is 600 Bar.

[0162] The details are as follows:

[0163] S7. Add 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, 3 g of embedded microspheres, and 0.15 g of lecithin to 200 mL of water, add 17 g of palm kernel oil, homogenize at 600 Bar high pressure for 15 min, and perform microfluidization at 100 MPa to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0164] Comparative Example 18

[0165] Compared with Example 3, the difference is that no microfluidization treatment is performed in step S7.

[0166] The details are as follows:

[0167] S7. Add 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, 3 g of embedded microspheres, and 0.15 g of lecithin to 200 mL of water, add 17 g of palm kernel oil, and homogenize at 450 Bar for 15 min to obtain an emulsion, which is freeze-dried to obtain a cell nutrition supplement.

[0168] Comparative Example 19

[0169] Compared with Example 3, the difference is that high-pressure homogenization treatment is not performed in step S7.

[0170] The details are as follows:

[0171] S7. 6 g of β-cyclodextrin, 5 g of arabinose, 1.5 g of antioxidant composition, 2.5 g of protein peptide chelate, 3 g of embedded microspheres, and 0.15 g of lecithin were added to 200 mL of water, and 17 g of palm kernel oil was added. The mixture was treated with a 100 MPa microjet to obtain an emulsion, which was freeze-dried to obtain a cell nutrition supplement.

[0172] Test Example 1

[0173] The zeta potential of the emulsions obtained in step S7 of Examples 1-4 or Comparative Examples 16-19 was measured using a dynamic light scattering instrument. The results are shown in Table 1.

[0174] Table 1

[0175]

[0176] As can be seen from the above table, the ζ-potential of the emulsion can be significantly improved and the stability is good after the high-pressure homogenization treatment and microfluidization treatment are used in Examples 1-4 of the present invention. The emulsion is broken when the pressure is low in Comparative Example 16, and the emulsion is also broken when a single microfluidization treatment is used. The ζ-potential value of Comparative Example 18 using a single high-pressure homogenization treatment decreases, and the ζ-potential value also decreases when the pressure is high in Comparative Example 19, because the emulsion particles repolymerize and the particle size becomes larger.

[0177] Test Example 2

[0178] The cell nutrition supplements prepared in Examples 1-4 of the present invention and Comparative Examples 1-15 were tested for nutritional components.

[0179] The carbohydrate content was determined by DNS method. Protein determination was carried out in accordance with GB 5009.5-2016 "National Food Safety Standard Determination of Protein in Food". Dietary fiber determination was carried out in accordance with GB 5009.88-2014 "National Food Safety Standard Determination of Dietary Fiber in Food". Selenium content was determined in accordance with GB5009.93-2017 "National Food Safety Standard Determination of Selenium in Food".

[0180] The results are shown in Table 2.

[0181] Table 2

[0182]

[0183] It can be seen from the above table that the cell nutrition supplements prepared in Examples 1-4 of the present invention are rich in protein content, high in selenium content, and high in nutritional value.

[0184] Test Example 3

[0185] Establishment and grouping of rat model of type II diabetes: Rats were randomly divided into normal group, modeling group, metformin group, Example 1-4 group, and Comparative Example 1-15 group, with 10 rats in each group. After one week of adaptation, the rats in the normal group were fed a standard diet, and the other rats were fed a high-fat and high-sugar feed for 30 days. Subsequently, the rats were intraperitoneally injected with streptozotocin (STZ) at 35 mg / kg for 3 times. Blood was collected from the tail vein, and the model was successfully established when the blood glucose level was >16.7 mmol / L. Subsequently, a standard diet was given, and the rats were gavaged with honey water, 10 g / kg oil and 10 mL / kg 42° liquor for 10 consecutive days to construct a rat model of type II diabetes.

[0186] Gavage: After the model was successfully established, the rats were gavaged for 1 month. The rats in the normal group and the model group were gavaged with 2 mL of normal saline; the metformin group was gavaged once a day at 4.17 mg / 100 g dissolved in 2 mL of normal saline; the Example 1-4 group and the Comparative Example 1-15 group were gavaged once a day at 0.5 g / 100 g. Tissue sample collection 1) Blood sample: After the gavage, the rats were anesthetized at a low dose and 2 mL of peritoneal venous blood was collected from the rats. The serum was separated by centrifugation at 3500 rpm for 10 min at 4°C. 2) Liver tissue sample: After the blood sample was collected, the rats were killed by dislocation of the neck, the liver tissue was exposed and dissected, 1 / 3 was separated and fixed in formalin, and the remaining part was divided into two and stored at -80°C for later use.

[0187] Blood glucose, serum insulin and HOMA-IR index: After the experiment, the fasting blood glucose (FBG) levels of rats in each group before and after gavage were measured using an Accu-Chek blood glucose meter after blood was collected from the tail vein. Blood from the rat peritoneal vein was collected to extract serum and centrifuged. The fasting insulin (FINS) of mice was measured using an ELISA kit. The insulin resistance homeostasis model was evaluated using the calculation formula: HOMA-IR = FBG (mmol / L) × FINS (mIU / mL) / 22.5. The results are shown in Table 3.

[0188] Table 3

[0189]

[0190] It can be seen from the above table that the cell nutrition supplements prepared in Examples 1-4 of the present invention can significantly reduce the HOMA-IR index of rats.

[0191] Blood lipid and serum inflammatory factor level detection: The rat peritoneal venous blood was collected, and the serum was separated by centrifugation. The TG, TC, and TNF-α levels of each group of rats after intragastric administration were detected using the corresponding ELISA kit. The liver tissue was separated, and 100 mg of liver tissue was added with an appropriate amount of physiological saline and homogenized on ice. After centrifugation and supernatant, the SOD content of liver tissue was determined using the corresponding kit. The results are shown in Table 4.

[0192] Table 4

[0193]

[0194] It can be seen from the above table that the cell nutrition supplements prepared in Examples 1-4 of the present invention can significantly reduce the content of TG and TC, reduce the content of TNF-, and increase the content of SOD.

[0195] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a cell nutrition supplement, characterized in that: Snail meat is enzymatically hydrolyzed to obtain snail protein peptides, which are mixed with composite protein hydrolysates obtained from kelp, ginseng and tau kueh powder, and chelated with chromium and zinc to obtain protein peptide chelates; kelp, ginseng and tau kueh powder are also used to prepare composite polysaccharides and antioxidant compositions, solid residues are added to water, fermented with selenium-enriched yeast and Bacillus licheniformis, the products are embedded with composite polysaccharides and sodium caseinate, and are homogenized under high pressure with β-cyclodextrin, arabinose, antioxidant composition, protein peptide chelates, lecithin and palm kernel oil, subjected to microfluidization treatment, freeze-dried and sterilized to obtain cell nutrition supplements; the steps include: S1. Add snail meat to water, mince, add trypsin, perform enzymolysis for the first time, filter, retain the solid, add composite protease to the filtrate, perform enzymolysis for the second time, dialyze, freeze-dry, and obtain snail protein peptide; the mass ratio of snail meat, trypsin, and composite protease is 10:0.5-1:1-2, the temperature of the first enzymolysis is 36-38°C, the time is 1-2h, the temperature of the second enzymolysis is 40-50°C, the time is 2-4h, and the composite protease is a mixture of neutral protease and ficin, and the mass ratio is 4-7:2; S2. The powder of Gexianmi algae is added to water to swell, frozen with liquid nitrogen, rewarmed at room temperature, and Baole fruit powder and ginseng powder are added, stirred and mixed evenly, snail enzyme is added to hydrolyze and break the wall, filtered, the solid is retained, the filtrate is subjected to ammonium sulfate graded precipitation, filtered, washed, and freeze-dried to obtain a composite protein, the filtrate is added with ethanol precipitation, filtered, washed, and dried to obtain a composite polysaccharide, the filtrate is recovered with ethanol, dialyzed to remove salt, and the liquid is dried to obtain an antioxidant composition; S3. adding the complex protein to water, adding protease to hydrolyze, and obtaining a complex protein peptide; S4. The composite protein peptide and snail protein peptide were mixed and added to water, chromium salt and zinc salt were added, stirred for reaction, dialyzed, and freeze-dried to obtain a protein peptide chelate; S5. The solid in step S1 and the solid in step S2 are mixed and added to water, inoculated with selenium-enriched yeast and Bacillus licheniformis, fermented and cultured, the product is filtered, the bacterial solution is washed and collected, mixed with the filtrate, and freeze-dried to obtain a fermentation-bacteria complex; S6. The complex polysaccharide and sodium caseinate were dissolved in water, the fermentation - bacteria complex and lecithin were added, the mixture was stirred and evenly mixed, the mixture was dropped into the fish oil, emulsified, and freeze-dried to obtain encapsulated microspheres; S7. Add β-cyclodextrin, arabinose, an antioxidant composition, protein peptide chelate, embedded microspheres, and lecithin into water, add palm kernel oil, perform high pressure homogenization, perform microfluidization, obtain an emulsion, freeze-dry, and sterilize to obtain a cell nutrition supplement; the mass ratio of β-cyclodextrin, arabinose, an antioxidant composition, protein peptide chelate, embedded microspheres, lecithin, and palm kernel oil is 5-7:4-6:1-2:2-3:2-4:0.1-0.2:15-20, the pressure of the high pressure homogenization is 400-500 Bar, the time is 10-20 min, and the pressure of the microfluidization treatment is 90-110 MPa.

2. The preparation method according to claim 1, characterized in that: In step S2, the solid-liquid ratio of the kudzu vine powder and water is 1:7-10 g / mL, the mass ratio of the kudzu vine powder, the taro fruit powder, the ginseng powder and the snail enzyme is 10-20:7-10:5-7:1-2, the temperature of the enzymolysis is 40-50°C, and the time is 1-3h.

3. The preparation method according to claim 1, characterized in that: In step S3, the mass ratio of the composite protein to the protease is 15-20:1-2, the protease is selected from at least one of neutral protease, ficin, bromelain, papain, alkaline protease, trypsin and pepsin, the enzymolysis temperature is 35-50° C., and the time is 30-60 min.

4. The preparation method according to claim 3, characterized in that: The protease is a mixture of bromelain and papain, with a mass ratio of 2-4:

3.

5. The preparation method according to claim 1, characterized in that: In step S4, the mass ratio of the composite protein peptide, snail protein peptide, chromium salt and zinc salt is 10-20:5-10:0.5-1:0.2-0.5, and the stirring reaction time is 20-40min.

6. The preparation method according to claim 1, characterized in that: In step S5, the mass ratio of the solid in step S1 to the solid in step S2 is 5:10-12, the inoculation amounts of the selenium-enriched yeast and Bacillus licheniformis are 2-3v / v% and 1-2v / v%, respectively, and the fermentation culture conditions are 40-45°C, 100-200r / min, and the fermentation culture is 24-48h.

7. The preparation method according to claim 1, characterized in that: The mass ratio of the complex polysaccharide, sodium caseinate, fermentation-bacteria complex and lecithin in step S6 is 4-6:7-10:5-7:0.1-0.

2.

8. A cell nutrition supplement prepared by the preparation method according to any one of claims 1 to 7.

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