A composition containing plant extracts and its application

Abstract

The present invention discloses a composition containing plant extracts and its application, and belongs to the technical field of dressing production. First, the composition containing plant extracts provided by the present invention is prepared by encapsulating plant extracts with liquid lipid materials and then freeze-drying. Then, the plant extracts provided by the present invention are composed of Hypericum perforatum extracts, okra extracts and broccoli seed extracts. Next, the present invention also provides methods for preparing Hypericum perforatum extracts and okra extracts. Finally, the present invention applies the prepared composition containing plant extracts in the field of dressings, showing excellent antibacterial properties.

Description

Technical Field

[0001] The invention belongs to the technical field of dressing production, and particularly relates to a composition containing plant extracts and application thereof. Background Art

[0002] Dressings are a type of medical material used to temporarily cover the surface of various wounds. They can be used to absorb wound exudate, stop bleeding, and promote wound healing. At the same time, they are coated on the surface of the wound to protect the wound from external bacterial infection. However, an ideal wound dressing should not only be able to serve as a barrier to isolate the wound from external bacteria, but also have good antibacterial properties.

[0003] Mycoporphyrin is an extract of Hypericum perforatum and the most biologically active substance in Hypericum perforatum. It is a grayish yellow to brown fluid powder, slightly bitter, almost insoluble in water, and can emit a special fragrance. It has the effects of inhibiting the central nervous system and calming down. It can enhance immunity when added to health products. At the same time, hypericin has excellent antiviral, anti-tumor and immunity-enhancing biological activities, and is often used in the medical field to develop antidepressant drugs. After searching, there is no relevant research on the use of hypericin in the field of medical dressings. Therefore, the present invention aims to apply hypericin to the field of dressings, aiming to solve the problem of poor antibacterial performance of traditional dressings. Summary of the invention

[0004] The present invention provides a technical solution for applying hypericin to the field of dressings. At the same time, the present invention further improves the preparation method of hypericin, and prepares a composition containing plant extracts by compounding multiple plant extracts and then coating with liquid lipids. The prepared composition containing plant extracts is then applied to the field of dressings, ultimately achieving the purpose of improving the antibacterial performance of dressings.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A composition containing plant extracts, prepared by encapsulating the plant extracts with a liquid lipid material and then freeze-drying;

[0007] The plant extract is composed of the following raw materials in percentage by mass:

[0008] Hypericum perforatum extract 80%-85%;

[0009] Okra extract 12%-15%;

[0010] Ginkgo extract 3% to 5%;

[0011] The Hypericum perforatum extract is prepared by the following steps:

[0012] The petals of Hypericum perforatum were rinsed three times with clean water, drained and placed in a drying oven at 40-50°C for 24 hours. After drying, the petals were taken out and ground through a 200-mesh sieve to obtain Hypericum perforatum powder. Tea saponin was added to a 60% ethanol aqueous solution by mass, and the mixture was stirred for 30 minutes to obtain an extraction solution. The Hypericum perforatum powder was added to the extraction solution, and the mixture was stirred for 1-2 hours. Ascorbic acid was then added thereto with stirring, and the mixture was transferred to a dark environment, and the mixture was stirred and extracted for 1-2 hours at a pressure of -0.1 to (-0.05) MPa. The mixture was filtered, and the filtrate was transferred to pure water, and then extracted with petroleum ether for 3-5 times. The petroleum ether was removed by reduced pressure distillation to obtain a crude Hypericum perforatum extract. The crude hypericin was adsorbed by LSA-5B macroporous adsorption resin, and then eluted with an 80% ethanol aqueous solution by mass. The eluate was collected and concentrated under reduced pressure to obtain a Hypericum perforatum extract.

[0013] Furthermore, the dosage ratio of the 60% ethanol aqueous solution, tea saponin, Hypericum perforatum powder and ascorbic acid is 1L: 8-10g: 25-30g: 4.5-5.5g.

[0014] Furthermore, the composition containing the plant extract is prepared by the following steps:

[0015] S1. Add polyethylene glycol monostearate to oleic acid, heat it to 65-70° C., stir at constant temperature for 3-5 min, then add plant extract thereto with stirring, heat it to 75-80° C., stir at constant temperature for 1-2 h, and then cool it to room temperature to obtain an oil phase;

[0016] S2, adding poloxamer 188 and soybean lecithin to pure water with stirring, heating to 65-70°C, stirring at constant temperature for 1-2 hours, and then cooling to room temperature to obtain an aqueous phase;

[0017] S3. Add the oil phase prepared above into the aqueous phase with stirring at a dropping rate of 1 to 2 drops / second at a mass ratio of 1:1. After the dropping is completed, stir for 5 to 6 hours to obtain a suspension. Place the suspension at -20°C for low-temperature solidification for 2 to 3 hours, filter through a 0.45 μm microporous membrane, take the filtrate to obtain a suspension, add 3% to 4% mannitol by mass of the suspension to the suspension, stir for 20 to 30 minutes, and then freeze-dry at -45 to (-40)°C for 2 to 3 hours to obtain a composition containing plant extracts.

[0018] Furthermore, the usage ratio of oleic acid, polyethylene glycol monostearate and plant extract in S1 is 100g:20-30g:45-50g.

[0019] Furthermore, the usage ratio of pure water, poloxamer 188 and soybean lecithin in S2 is 100g:1.5-2g:4-5g.

[0020] Furthermore, the okra extract is prepared by the following steps:

[0021] The okra is washed and cut into sections, and microwave-dried in a microwave vacuum dryer at a microwave power of 480 to 500 W for a drying time of 3 to 5 min. The okra is then vacuum-freeze-dried in a vacuum freeze dryer at a vacuum freeze-drying temperature of -35 to (-20) ° C, a vacuum degree of 28 to 30 Pa, and a drying time of 6 to 8 h to obtain dried okra. The dried okra is crushed and passed through a 200-mesh sieve to obtain okra powder. An ethanol aqueous solution with a mass fraction of 60% is added to the okra powder at a solid-liquid ratio of 1:25 to 30, and then ultrasonically extracted at 70 to 80 ° C for 30 min. The extract is collected and concentrated under reduced pressure to obtain an okra extract. The okra extract is extracted with ethyl acetate and n-butanol in sequence, and the extract is collected. The extract is concentrated under reduced pressure to obtain an okra extract.

[0022] Furthermore, the use of the above composition in a dressing comprises the following steps:

[0023] First, the dressing includes the following raw materials by weight:

[0024] 18.5-20.5 parts of a composition containing a plant extract;

[0025] 7-10 parts of hyaluronic acid;

[0026] Chitosan 4.5-5.5 parts;

[0027] 2-3 parts of polyol;

[0028] 0.1-0.15 parts of preservatives;

[0029] 35-40 parts of pure water;

[0030] Then, the preparation method of the dressing comprises the following steps:

[0031] Weigh the raw materials according to the above-mentioned mass parts, first add hyaluronic acid, chitosan and polyol into pure water with stirring, mix and stir for 20 to 30 minutes, then add the composition containing plant extracts and preservatives therein with stirring, mix and stir for 0.5 to 1 hour, and then fill and sterilize to obtain a dressing.

[0032] Furthermore, the polyol is one of glycerol and propylene glycol.

[0033] Furthermore, the preservative is one of glyceryl caprylate, caprylhydroxamic acid and methylparaben.

[0034] Beneficial effects of the present invention:

[0035] The present invention provides a composition containing plant extracts and its application in dressings. First, the composition containing plant extracts provided by the present invention is prepared by encapsulating plant extracts with liquid lipid materials and then freeze-drying. Then, the plant extracts provided by the present invention are composed of Hypericum perforatum extracts, okra extracts and Ginkgo extracts. Next, the present invention also provides methods for preparing Hypericum perforatum extracts and okra extracts. Finally, the present invention applies the prepared composition containing plant extracts in the field of dressings, showing excellent antibacterial properties.

[0036] First of all, the main ingredient of Hypericum perforatum extract is hypericin. Hypericin can be excited by visible light and then rely on the participation of oxygen to produce active oxygen substances such as singlet oxygen through type I and type II reactions, thereby achieving sterilization and inactivation of toxin factors; then, okra extract has excellent antioxidant, anti-inflammatory and immunomodulatory effects. Most importantly, okra extract has a moisturizing effect and can promote wound recovery; finally, Ginkgo extract is rich in flavonoids and terpenoid lactones, which have anti-inflammatory, microcirculation-improving and neuroprotective effects. Improving microcirculation means improving blood circulation in the wound area, thereby accelerating the wound healing process by promoting the supply of oxygen and nutrients. The present invention combines Hypericum perforatum extract, okra extract and Ginkgo biloba extract, and then applies them to the field of dressings. After testing, it shows excellent synergistic effect, and the reasons may be as follows: first, the antibacterial property of hypericin is combined with the anti-inflammatory property of okra and Ginkgo biloba extracts to enhance the antibacterial effect; then, the moisturizing effect of okra extract and the microcirculation improvement effect of Ginkgo biloba extract can create an environment conducive to wound healing. The three effects have the effect of complementing each other and complementing each other. Selecting the combination of Hypericum perforatum extract, okra extract and Ginkgo biloba extract is one of the main innovations of the present application.

[0037] However, the active factors in the Hypericum perforatum extract, okra extract and ginkgo extract are all sensitive to light and need to be stored away from light. Therefore, the present invention uses liquid lipid material polyethylene glycol monostearate (PEG-MS) to encapsulate the above composition. PEG-MS has low irritation to the skin and mucous membranes and has a moisturizing effect. It is stable and safe during storage. After being applied to the skin surface as a dressing, PEG-MS is more sensitive to temperature and pH. With the transmission of the skin temperature on the human body surface and the effect of the slightly acidic environment of the wound, the interaction of the skin surface lipids and the hydrolysis of PEG-MS itself finally realize the release of the plant extracts encapsulated inside. The encapsulation of PEG-MS improves the bioavailability of the active factors in the Hypericum perforatum extract, okra extract and ginkgo extract, which is also one of the main innovations of this application.

[0038] Next, the present invention improves the preparation methods of Hypericum perforatum extract and Okra extract. In the preparation process of Hypericum perforatum extract, the present invention uses tea saponin as a surfactant to extract Hypericum perforatum powder under negative pressure conditions. On the one hand, tea saponin is a natural surfactant, and its surface contains many hydroxyl groups, which can form hydrogen bonds with hypericin, thereby improving the extraction rate of hypericin. On the other hand, during the negative pressure extraction process, the solution in the system moves violently, and the mass transfer rate of hypericin in the solvent is increased under turbulence and suspension. Therefore, the improved extraction method of the present invention is more efficient. Among them, in the preparation process of okra extract, the present invention combines microwave treatment and vacuum freezing treatment. Microwave treatment can quickly reduce the moisture in okra, but there is also a problem of uneven temperature. Under vacuum freeze-drying conditions, polyphenol oxidase in okra is inhibited to a certain extent. Therefore, the present invention combines the two, strictly controls the microwave treatment power and time, and at the same time, strictly controls the vacuum freezing time, ensures the drying efficiency of okra, and at the same time increases the content level of polyphenol active substances in the okra extract, which is ultimately manifested in the improvement of antibacterial performance.

[0039] Finally, the present invention provides the use of the composition containing plant extracts prepared above in dressings, and the dressings are endowed with excellent antibacterial properties by adding the composition containing plant extracts and reasonable compatibility. DETAILED DESCRIPTION

[0040] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Example 1

[0042] Preparation of Hypericum perforatum extract:

[0043] First, rinse the petals of Hypericum perforatum three times with clean water, drain and dry them in a 40°C drying oven for 24 hours. After drying, take them out and grind them through a 200-mesh sieve to obtain Hypericum perforatum powder. Then add 8g of tea saponin to 1L of 60% ethanol aqueous solution, stir and mix for 30 minutes to obtain an extraction solution, add 25g of Hypericum perforatum powder to the extraction solution, stir and mix for 1 hour, and then stir and add 4.5g of ascorbic acid thereto, transfer to a dark environment, stir and extract for 1 hour at a pressure of -0.05MPa, filter, transfer the filtrate to pure water, extract with petroleum ether for 3 times, and then remove the petroleum ether by reduced pressure distillation to obtain a crude Hypericum perforatum extract, i.e., gold The crude hypericin was adsorbed by LSA-5B macroporous adsorption resin, and then eluted with 80% ethanol aqueous solution, the eluate was collected, and then concentrated under reduced pressure to obtain purified hypericin, that is, the Hypericum perforatum extract, wherein the 60% ethanol aqueous solution and the 80% ethanol aqueous solution were both prepared from anhydrous ethanol and pure water; wherein anhydrous ethanol (AR), ascorbic acid (GR) and petroleum ether (60-90°C; AR) were purchased from Sinopharm Chemical Reagent Co., Ltd.; wherein tea saponin was purchased from Shaanxi Haochen Biotechnology Co., Ltd.; wherein LSA-5B macroporous adsorption resin was purchased from Jining Fangyu Chemical Co., Ltd.

[0044] Example 2

[0045] Preparation of Hypericum perforatum extract:

[0046] First, rinse the petals of Hypericum perforatum with clean water three times, drain and dry them in a 45°C drying oven for 24 hours. After drying, take them out and grind them through a 200-mesh sieve to obtain Hypericum perforatum powder. Then add 10g of tea saponin to 1L of 60% ethanol aqueous solution by mass fraction, stir and mix for 30 minutes to obtain an extraction solution, add 30g of Hypericum perforatum powder to the extraction solution, stir and mix for 2 hours, and then stir and add 5g of ascorbic acid thereto, and then transfer to a dark environment, stir and extract for 2 hours under a pressure of -0.1MPa, filter, transfer the filtrate to pure water, extract with petroleum ether for 5 times, and then remove the petroleum ether by reduced pressure distillation to obtain a crude Hypericum perforatum extract, i.e., golden silk. The crude product of hypericin was adsorbed by LSA-5B macroporous adsorption resin, and then eluted with 80% ethanol aqueous solution, the eluate was collected, and then concentrated under reduced pressure to obtain purified hypericin, that is, the Hypericum perforatum extract, wherein the 60% ethanol aqueous solution and the 80% ethanol aqueous solution were both prepared from anhydrous ethanol and pure water; wherein anhydrous ethanol (AR), ascorbic acid (GR) and petroleum ether (60-90°C; AR) were purchased from Sinopharm Chemical Reagent Co., Ltd.; wherein tea saponin was purchased from Shaanxi Haochen Biotechnology Co., Ltd.; wherein LSA-5B macroporous adsorption resin was purchased from Jining Fangyu Chemical Co., Ltd.

[0047] Example 3

[0048] Preparation of Hypericum perforatum extract:

[0049] First, rinse the petals of Hypericum perforatum with clean water three times, drain and place in a 50°C drying oven to dry for 24 hours. After drying, take out and grind through a 200-mesh sieve to obtain Hypericum perforatum powder, then add 10g of tea saponin to 1L of 60% ethanol aqueous solution, stir and mix for 30 minutes to obtain an extraction solution, add 30g of Hypericum perforatum powder to the extraction solution, stir and mix for 2 hours, then stir and add 5.5g of ascorbic acid thereto, transfer to light-proof conditions, stir and extract for 2 hours at a pressure of -0.1MPa, filter, transfer the filtrate to pure water, extract with petroleum ether 5 times, and then remove the petroleum ether by reduced pressure distillation to obtain a crude Hypericum perforatum extract, i.e., gold The crude hypericin was adsorbed by LSA-5B macroporous adsorption resin, and then eluted with 80% ethanol aqueous solution, the eluate was collected, and then concentrated under reduced pressure to obtain purified hypericin, that is, the Hypericum perforatum extract, wherein the 60% ethanol aqueous solution and the 80% ethanol aqueous solution were both prepared from anhydrous ethanol and pure water; wherein anhydrous ethanol (AR), ascorbic acid (GR) and petroleum ether (60-90°C; AR) were purchased from Sinopharm Chemical Reagent Co., Ltd.; wherein tea saponin was purchased from Shaanxi Haochen Biotechnology Co., Ltd.; wherein LSA-5B macroporous adsorption resin was purchased from Jining Fangyu Chemical Co., Ltd.

[0050] Example 4

[0051] Prepare Okra Extract:

[0052] First, the okra was washed and cut into sections, and microwave dried in a microwave vacuum dryer with a microwave power of 480w and a drying time of 3min. Then, vacuum freeze drying was performed in a vacuum freeze dryer at a temperature of -35℃, a vacuum degree of 28Pa, and a drying time of 6h to obtain dried okra. The dried okra was crushed and passed through a 200-mesh sieve to obtain okra powder. A 60% ethanol aqueous solution was added to the okra powder at a solid-liquid ratio of 1:25, and then ultrasonically extracted at 70℃ for 30min. The extract was collected and concentrated under reduced pressure to obtain an okra extract. The okra extract was extracted with ethyl acetate and n-butanol in turn, and the extract was collected. The extract was concentrated under reduced pressure to obtain an okra extract, wherein the 60% ethanol aqueous solution was prepared from anhydrous ethanol and pure water; wherein anhydrous ethanol (AR), ethyl acetate (AR) and n-butanol (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0053] Example 5

[0054] Prepare Okra Extract:

[0055] First, the okra was washed and cut into sections, and microwave dried in a microwave vacuum dryer with a microwave power of 500 w and a drying time of 5 min. Then, vacuum freeze drying was performed in a vacuum freeze dryer at a temperature of -30 °C, a vacuum degree of 30 Pa, and a drying time of 8 h to obtain dried okra. The dried okra was crushed and passed through a 200-mesh sieve to obtain okra powder. A 60% ethanol aqueous solution was added to the okra powder at a solid-liquid ratio of 1:28, and then ultrasonically extracted at 80 °C for 30 min. The extract was collected and concentrated under reduced pressure to obtain an okra extract. The okra extract was extracted with ethyl acetate and n-butanol in turn, and the extract was collected. The extract was concentrated under reduced pressure to obtain an okra extract, wherein the 60% ethanol aqueous solution was prepared from anhydrous ethanol and pure water; wherein anhydrous ethanol (AR), ethyl acetate (AR) and n-butanol (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0056] Example 6

[0057] Prepare Okra Extract:

[0058] First, the okra was washed and cut into sections, and microwave dried in a microwave vacuum dryer with a microwave power of 500 w and a drying time of 5 min. Then, vacuum freeze drying was performed in a vacuum freeze dryer at a temperature of -20 °C, a vacuum degree of 30 Pa, and a drying time of 8 h to obtain dried okra. The dried okra was crushed and passed through a 200-mesh sieve to obtain okra powder. A 60% ethanol aqueous solution was added to the okra powder at a solid-liquid ratio of 1:30, and then ultrasonically extracted at 80 °C for 30 min. The extract was collected and concentrated under reduced pressure to obtain an okra extract. The okra extract was extracted with ethyl acetate and n-butanol in turn, and the extract was collected. The extract was concentrated under reduced pressure to obtain an okra extract, wherein the 60% ethanol aqueous solution was prepared from anhydrous ethanol and pure water; wherein anhydrous ethanol (AR), ethyl acetate (AR) and n-butanol (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0059] Example 7

[0060] Preparation of compositions containing plant extracts:

[0061] S1. Add 100 g of oleic acid and 20 g of polyethylene glycol monostearate to reactor A, heat reactor A to 65° C., stir at constant temperature for 3 min, then add 45 g of plant extract thereto with stirring, heat reactor A to 75° C., stir at constant temperature for 1 h, and then cool to room temperature to obtain an oil phase, wherein the plant extract is composed of the following raw materials in mass percentage: 80% of the Hypericum perforatum extract prepared in Example 1, 15% of the okra extract prepared in Example 4, and 5% of the ginkgo extract, wherein oleic acid (reagent grade) and polyethylene glycol monostearate (n≈10) are purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; wherein the ginkgo extract is purchased from Croda Chemicals (Shanghai) Co., Ltd.

[0062] S2. Add 100 g of pure water to reactor B, and then add 1.5 g of poloxamer 188 and 4 g of soybean lecithin with stirring. Heat reactor B to 65° C., stir at constant temperature for 1 h, and then cool to room temperature to obtain an aqueous phase, wherein poloxamer 188 and soybean lecithin (biotechnology grade) are purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0063] S3. Add the oil phase prepared above to the aqueous phase with stirring at a dropping rate of 2 drops / second in a mass ratio of 1:1. After the addition is completed, stir for 5 hours to obtain a suspension. Place the suspension at -20°C for low-temperature solidification for 2 hours, filter through a 0.45 μm microporous membrane, take the filtrate to obtain a suspension, add 3% mannitol by mass of the suspension to the suspension with stirring, stir for 20 minutes, and then freeze-dry at -45°C for 2 hours to obtain a composition containing plant extracts, wherein the microporous membrane is purchased from Sinopharm Chemical Reagent Co., Ltd.; wherein mannitol (D-mannitol; reagent grade) is purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0064] Example 8

[0065] Preparation of compositions containing plant extracts:

[0066] S1. Add 100 g of oleic acid and 25 g of polyethylene glycol monostearate to reactor A, heat reactor A to 70° C., stir at constant temperature for 5 min, then add 50 g of plant extract thereto with stirring, heat reactor A to 80° C., stir at constant temperature for 2 h, and then cool to room temperature to obtain an oil phase, wherein the plant extract is composed of the following raw materials in mass percentage: 82% of the Hypericum perforatum extract prepared in Example 2, 14% of the okra extract prepared in Example 5, and 4% of the ginkgo extract, wherein oleic acid (reagent grade) and polyethylene glycol monostearate (n≈10) are purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; wherein the ginkgo extract is purchased from Croda Chemicals (Shanghai) Co., Ltd.

[0067] S2. Add 100 g of pure water to reactor B, and then add 1.8 g of poloxamer 188 and 5 g of soybean lecithin with stirring. Heat reactor B to 70° C. and stir at this constant temperature for 2 h. Then cool to room temperature to obtain an aqueous phase. Poloxamer 188 and soybean lecithin (biotechnology grade) were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0068] S3. The prepared oil phase is added dropwise to the aqueous phase at a drop rate of 1 drop / second at a mass ratio of 1:1 with stirring. After the addition is completed, the suspension is stirred for 6 hours to obtain a suspension. The suspension is placed at -20°C for low-temperature solidification for 3 hours, and then filtered through a 0.45 μm microporous membrane to obtain a suspension. Mannitol in an amount of 4% by mass of the suspension is added to the suspension with stirring, and stirred for 30 minutes. The suspension is then placed at -45°C for freeze-drying for 3 hours to obtain a composition containing plant extracts, wherein the microporous membrane is purchased from Sinopharm Chemical Reagent Co., Ltd.; and mannitol (D-mannitol; reagent grade) is purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0069] Example 9

[0070] Preparation of compositions containing plant extracts:

[0071] S1. Add 100 g of oleic acid and 30 g of polyethylene glycol monostearate to reactor A, heat reactor A to 70° C., stir at constant temperature for 5 min, then add 50 g of plant extract thereto with stirring, heat reactor A to 80° C., stir at constant temperature for 2 h, and then cool to room temperature to obtain an oil phase, wherein the plant extract is composed of the following raw materials in mass percentage: 85% of the Hypericum perforatum extract prepared in Example 3, 12% of the okra extract prepared in Example 6, and 3% of the ginkgo extract, wherein oleic acid (reagent grade) and polyethylene glycol monostearate (n≈10) are purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; wherein the ginkgo extract is purchased from Croda Chemicals (Shanghai) Co., Ltd.

[0072] S2. Add 100 g of pure water to reactor B, and then add 2 g of poloxamer 188 and 5 g of soybean lecithin with stirring. Heat reactor B to 70° C. and stir at this constant temperature for 2 h. Then cool to room temperature to obtain an aqueous phase. Poloxamer 188 and soybean lecithin (biotechnology grade) were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0073] S3. Add the oil phase prepared above into the aqueous phase with stirring at a dropping rate of 1 drop / second and a mass ratio of 1:1. After the dropping is completed, stir for 6 hours to obtain a suspension. Place the suspension at -20°C for low-temperature solidification for 3 hours, filter through a 0.45 μm microporous membrane, take the filtrate to obtain a suspension, add 4% mannitol by mass of the suspension to the suspension, stir for 30 minutes, and then freeze-dry at -40°C for 3 hours to obtain a composition containing plant extracts, wherein the microporous membrane is purchased from Sinopharm Chemical Reagent Co., Ltd.; wherein mannitol (D-mannitol; reagent grade) is purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0074] Comparative Example 1

[0075] Comparative Example 1 is the control group of Example 8, except that the Hypericum perforatum extract prepared in Example 2 in the plant extract in Example 8 is removed, that is, the plant extract in Example 8 is replaced by the okra extract and the ginkgo extract prepared in Example 5 mixed in a mass ratio of 7:2, and the other raw materials, raw material amounts and preparation methods remain unchanged, and finally a composition containing plant extracts is obtained.

[0076] Comparative Example 2

[0077] Comparative Example 2 is the control group of Example 8, wherein the okra extract prepared in Example 5 is removed from the plant extract in Example 8, that is, the plant extract in Example 8 is replaced by the Hypericum perforatum extract and the Ginkgo biloba extract prepared in Example 2 in a mass ratio of 41:2, and the other raw materials, raw material amounts and preparation methods remain unchanged, thereby finally obtaining a composition containing a plant extract.

[0078] Comparative Example 3

[0079] Comparative Example 3 is the control group of Example 8, except that the ginkgo extract in the plant extract in Example 8 is removed, that is, the plant extract in Example 8 is replaced by the Hypericum perforatum extract prepared in Example 2 and the okra extract prepared in Example 5, which are mixed in a mass ratio of 41:7, and the other raw materials, raw material amounts and preparation methods remain unchanged, and finally a composition containing plant extracts is obtained.

[0080] Example 10

[0081] The application of the composition containing the plant extract in a dressing comprises the following steps:

[0082] First, the dressing includes the following raw materials by weight:

[0083] 20.5 parts of the composition containing plant extract prepared in Example 7;

[0084] 10 parts of hyaluronic acid;

[0085] Chitosan 5.5 parts;

[0086] 3 parts of glycerin;

[0087] Glyceryl caprylate 0.15 parts;

[0088] 40 parts of pure water.

[0089] Then, the preparation method of the dressing comprises the following steps:

[0090] The raw materials were weighed according to the above mass parts. First, hyaluronic acid, chitosan and glycerol were added to pure water with stirring, and the mixture was mixed and stirred for 20 minutes. Then, the composition containing plant extracts prepared in Example 7 and glyceryl caprylate were added thereto with stirring, and the mixture was mixed and stirred for 0.5 hours. The dressing was then filled and sterilized to obtain a dressing, wherein hyaluronic acid (97%), chitosan (AR), glycerol (AC) and glyceryl caprylate (Type II standard product) were all purchased from Sinopharm Chemical Reagent Co., Ltd.

[0091] Embodiment 11

[0092] The application of the composition containing the plant extract in a dressing comprises the following steps:

[0093] First, the dressing includes the following raw materials by weight:

[0094] 20 portions of the composition containing plant extracts prepared in Example 8;

[0095] 8 parts of hyaluronic acid;

[0096] Chitosan 5 parts;

[0097] 3 parts of propylene glycol;

[0098] 0.1 part of octanoylhydroxamic acid;

[0099] 36 parts of pure water.

[0100] Then, the preparation method of the dressing comprises the following steps:

[0101] The raw materials were weighed according to the above mass parts. First, hyaluronic acid, chitosan, and polyols glycerol and propylene glycol were added to pure water with stirring, and the mixture was mixed and stirred for 30 minutes. Then, the composition containing plant extracts prepared in Example 8 and preservatives glyceryl caprylate, caprylhydroxamic acid, and methylparaben were added thereto with stirring, and the mixture was mixed and stirred for 1 hour. The dressing was then filled and sterilized to obtain a dressing, wherein hyaluronic acid (97%), chitosan (AR), and propylene glycol (AR) were all purchased from Sinopharm Chemical Reagent Co., Ltd.; and caprylhydroxamic acid (99%) was purchased from Hubei Keward Chemical Co., Ltd.

[0102] Example 12

[0103] The application of the composition containing the plant extract in a dressing comprises the following steps:

[0104] First, the dressing includes the following raw materials by weight:

[0105] 18.5 parts of the composition containing plant extract prepared in Example 9;

[0106] 7 parts of hyaluronic acid;

[0107] Chitosan 4.5 parts;

[0108] 2 parts of propylene glycol;

[0109] Methylparaben 0.1 part;

[0110] 35 parts of pure water.

[0111] Then, the preparation method of the dressing comprises the following steps:

[0112] The raw materials were weighed according to the above mass parts. First, hyaluronic acid, chitosan and propylene glycol were added to pure water with stirring, and the mixture was mixed and stirred for 30 minutes. Then, the composition containing plant extracts prepared in Example 9 and preservatives glyceryl caprylate, caprylhydroxamic acid and methylparaben were added thereto with stirring, and the mixture was mixed and stirred for 1 hour. The dressing was then filled and sterilized to obtain a dressing, wherein hyaluronic acid (97%), chitosan (AR), propylene glycol (AR) and methylparaben (standard product of methylparaben) were all purchased from Sinopharm Chemical Reagent Co., Ltd.

[0113] Comparative Example 4

[0114] Comparative Example 4 is the control group of Example 11, in which the composition containing plant extracts prepared in Example 8 of Example 11 is replaced by the composition containing plant extracts prepared in Comparative Example 1, and the other raw materials, raw material amounts and preparation methods remain unchanged, to finally obtain a dressing.

[0115] Comparative Example 5

[0116] Comparative Example 5 is the control group of Example 11, in which the composition containing plant extracts prepared in Example 8 of Example 11 is replaced by the composition containing plant extracts prepared in Comparative Example 2, and the other raw materials, raw material amounts and preparation methods remain unchanged, to finally obtain a dressing.

[0117] Comparative Example 6

[0118] Comparative Example 6 is the control group of Example 11, in which the composition containing plant extracts prepared in Example 8 of Example 11 is replaced by the composition containing plant extracts prepared in Comparative Example 3, and the other raw materials, raw material amounts and preparation methods remain unchanged, to finally obtain a dressing.

[0119] Comparative Example 7

[0120] Comparative Example 7 is the control group of Example 11, wherein the composition containing the plant extract prepared in Example 8 in Example 11 is replaced by the plant extract in S1 in Example 8, that is, the packaging and freeze-drying processes are removed, that is, the plant extract prepared in Example 8 in Example 11 is replaced by a plant extract composed of the following raw materials in percentage by mass: 82% of the Hypericum perforatum extract prepared in Example 2, 14% of the okra extract prepared in Example 5, and 4% of the Ginkgo extract, and the other raw materials, the amounts of the raw materials used, and the preparation method remain unchanged, and a dressing is finally obtained.

[0121] The dressings prepared in Examples 10 to 12 and Comparative Examples 4 to 7 were subjected to performance tests. The performance test process is as follows, and the test results are shown in Table 1:

[0122] (1) Preparation of dressing patch: The dressing is impregnated in a base film, which is a round film made of viscose fiber. The base film impregnated with the dressing is sealed in an aluminum foil bag to obtain a dressing patch.

[0123] (2) Antibacterial performance test: Prepare bacterial liquid, select Escherichia coli (Gram-negative bacillus) and Staphylococcus aureus (Gram-positive coccus) to study the antibacterial performance of the dressing. First, add the stock solution of Escherichia coli and Staphylococcus aureus to 20 mL of sterilized nutrient broth, and then place it in a constant temperature shaker (100 rpm, 37°C) and culture it for 24 hours to obtain the bacterial liquid of Escherichia coli and Staphylococcus aureus. Then, soak the sterilized dressing (cut into discs with a diameter of 10 mm) in the bacterial liquid (10 7 CFU / mL), and cultured at 37°C for 30 min as the experimental group. At the same time, the bacterial solution without any treatment was used as the blank control group. 500 μL of the diluted bacterial solution of different groups was evenly dispersed on the LB agar plate and cultured at 37°C for 24 h. The number of bacterial colonies was counted by the plate counting method, and the inhibition rate was calculated by the following formula:

[0124] Antibacterial rate (%) = (N a -N b )×100% / N a ;

[0125] Among them, N a is the colony count of the blank control group; N b is the colony number of the experimental group.

[0126] Table 1

[0127] project Example 10 Embodiment 11 Example 12 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Antibacterial rate (Escherichia coli) / % 98.1 98.9 98.6 87.5 95.0 96.7 91.4 Antibacterial rate (Staphylococcus aureus) / % 97.0 98.1 97.7 85.4 93.6 95.4 90.2

[0128] As can be seen from Table 1, the present invention combines Hypericum perforatum extract, Okra extract and Ginkgo extract, and then applies them to the field of dressings. After testing, it shows excellent synergistic effect in antibacterial performance. At the same time, after the plant extracts are wrapped with liquid lipid materials, the antibacterial performance of the dressing can be significantly improved.

[0129] It should be noted that, in this article, terms such as "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or equipment that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or equipment.

[0130] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composition containing a plant extract, characterized in that It is prepared by encapsulating plant extracts with liquid lipid materials and then freeze-drying; The plant extract is composed of the following raw materials in percentage by mass: Hypericum perforatum extract 80%-85%; Okra extract 12%-15%; Ginkgo extract 3% to 5%; The Hypericum perforatum extract is prepared by the following steps: The petals of Hypericum perforatum were rinsed three times with clean water, drained and placed in a drying oven at 40-50°C for 24 hours. After drying, the petals were taken out and ground through a 200-mesh sieve to obtain Hypericum perforatum powder. Tea saponin was added to a 60% ethanol aqueous solution by mass, and the mixture was stirred for 30 minutes to obtain an extraction solution. The Hypericum perforatum powder was added to the extraction solution, and the mixture was stirred for 1-2 hours. Ascorbic acid was then added thereto with stirring, and the mixture was transferred to a dark environment, and the mixture was stirred and extracted for 1-2 hours at a pressure of -0.1 to (-0.05) MPa. The mixture was filtered, and the filtrate was transferred to pure water. The mixture was extracted with petroleum ether for 3-5 times, and the petroleum ether was removed by reduced pressure distillation to obtain a crude Hypericum perforatum extract, i.e., a crude hypericin product. The crude hypericin product was adsorbed by LSA-5B macroporous adsorption resin, and then eluted with an 80% ethanol aqueous solution by mass. The eluate was collected and concentrated by reduced pressure to obtain a Hypericum perforatum extract.

2. A composition containing plant extracts according to claim 1, characterized in that: The dosage ratio of the 60% ethanol aqueous solution, tea saponin, Hypericum perforatum powder and ascorbic acid is 1L: 8-10g: 25-30g: 4.5-5.5g.

3. A composition containing plant extracts according to claim 1, characterized in that: The composition containing the plant extract is prepared by the following steps: S1. Add polyethylene glycol monostearate to oleic acid, heat it to 65-70° C., stir at constant temperature for 3-5 min, then add plant extract thereto with stirring, heat it to 75-80° C., stir at constant temperature for 1-2 h, and then cool it to room temperature to obtain an oil phase; S2, adding poloxamer 188 and soybean lecithin to pure water with stirring, heating to 65-70°C, stirring at constant temperature for 1-2 hours, and then cooling to room temperature to obtain an aqueous phase; S3. Add the oil phase prepared above into the aqueous phase with stirring at a dropping rate of 1 to 2 drops / second at a mass ratio of 1:

1. After the dropping is completed, stir for 5 to 6 hours to obtain a suspension. Place the suspension at -20°C for low-temperature solidification for 2 to 3 hours, filter through a 0.45 μm microporous membrane, take the filtrate to obtain a suspension, add 3% to 4% mannitol by mass of the suspension to the suspension, stir for 20 to 30 minutes, and then freeze-dry at -45 to (-40)°C for 2 to 3 hours to obtain a composition containing plant extracts.

4. A composition containing plant extracts according to claim 3, characterized in that: The usage ratio of oleic acid, polyethylene glycol monostearate and plant extract in S1 is 100g:20-30g:45-50g.

5. A composition containing plant extracts according to claim 3, characterized in that: The usage ratio of pure water, poloxamer 188 and soybean lecithin in S2 is 100g:1.5-2g:4-5g.

6. A composition containing plant extracts according to claim 1, characterized in that: The okra extract is prepared by the following steps: The okra is washed and cut into sections, and microwave-dried in a microwave vacuum dryer at a microwave power of 480 to 500 W for a drying time of 3 to 5 min. The okra is then vacuum-freeze-dried in a vacuum freeze dryer at a vacuum freeze-drying temperature of -35 to (-20) ° C, a vacuum degree of 28 to 30 Pa, and a drying time of 6 to 8 h to obtain dried okra. The dried okra is crushed and passed through a 200-mesh sieve to obtain okra powder. An ethanol aqueous solution with a mass fraction of 60% is added to the okra powder at a solid-liquid ratio of 1:25 to 30, and then ultrasonically extracted at 70 to 80 ° C for 30 min. The extract is collected and concentrated under reduced pressure to obtain an okra extract. The okra extract is extracted with ethyl acetate and n-butanol in sequence, and the extract is collected. The extract is concentrated under reduced pressure to obtain an okra extract.

7. Use of a composition containing a plant extract, characterized in that: The use of the composition according to any one of claims 1 to 6 in preparing a dressing comprises the following steps: First, the dressing includes the following raw materials by weight: 18.5-20.5 parts of a composition containing a plant extract; 7-10 parts of hyaluronic acid; Chitosan 4.5-5.5 parts; 2-3 parts of polyol; 0.1-0.15 parts of preservatives; 35-40 parts of pure water; Then, the preparation method of the dressing comprises the following steps: Weigh the raw materials according to the above-mentioned mass parts, first add hyaluronic acid, chitosan and polyol into pure water with stirring, mix and stir for 20 to 30 minutes, then add the composition containing plant extracts and preservatives therein with stirring, mix and stir for 0.5 to 1 hour, and then fill and sterilize to obtain a dressing.

8. The use of a composition containing plant extracts according to claim 7, characterized in that: The polyol is one of glycerol and propylene glycol.

9. The use of a composition containing plant extracts according to claim 7, characterized in that: The preservative is one of glyceryl caprylate, caprylhydroxamic acid and methylparaben.