Hyperbaric oxygen device capable of prolonging service life and improving physiological function and application and method thereof
By using high-purity oxygen gas in the hyperbaric oxygen chamber for hyperbaric oxygen therapy, the effectiveness and safety of extending life and improving healthy lifespan in the prior art were solved, significantly prolonging the lifespan of mice and improving physiological functions, especially In high-dose X-ray mice, the health of an aging society has been improved.
Patent Information
- Application Number
- CN202411553314.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-01
- Publication Date
- 2024-12-13
AI Technical Summary
The prior art has effectiveness and safety problems in extending lifespan and improving healthy lifespan, especially in human applications. High dose X-rays are harmful to human health and lack effective methods to extend lifespan and improve physiological functions.
Provide a high-pressure oxygen device and method, by using high-purity oxygen in the high-pressure oxygen chamber for pressure regulation, set pressure 1.0-3.0ATA, oxygen concentration ≥92%, time 90-180 minutes, repeated treatment to extend life and improve physiology Functions, including grip strength, cognition, memory, inflammation levels, and oxidative stress levels.
Effectively extend the lifespan of elderly mice and high-dose X-ray mice, improve physiological functions, and provide a safe and easy-to-implement method to extend healthy lifespan and improve the health challenges of an aging society.
Smart Images
Figure CN119112558A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a device for treating or improving tumors and age-related diseases and an application and method thereof, belonging to the technical field of biomedicine. Background Art
[0002] With the acceleration of the aging process of the global population, how to promote individual health and longevity has become a major challenge facing today's society. In addition to its classic meaning, that is, the length of time an individual experiences from birth to death, lifespan is also closely related to the concept of healthspan, which refers to the length of time an individual maintains a healthy state throughout his life. Improving healthspan can indirectly extend lifespan by reducing the occurrence and development of diseases. The importance of promoting health and longevity is not only reflected in the increase in personal lifespan, but also in the improvement of quality of life. It is also of great significance to reduce the pressure on the public health system.
[0003] In recent years, researchers have discovered a variety of interventions that can potentially extend lifespan and healthspan, including dietary restrictions, small molecule drugs, and gene therapy. However, most studies are still in the early stages and face some key technical and theoretical challenges. The effects of many interventions have been verified in model organisms, but their effects in humans have not been fully confirmed. For example, although some studies have confirmed that calorie restriction can significantly extend the lifespan of model organisms, the feasibility of this approach in humans is unclear and may be accompanied by some adverse quality of life effects. In addition, although the various small molecule drugs currently under study (such as metformin and rapamycin) have broad prospects, issues such as their safety, efficacy, and long-term effects remain to be resolved. While some new interventions in the research stage, including gene therapy, stem cell therapy, and young plasma infusion, have shown great potential to promote healthy aging in basic research, they face important ethical considerations in the process of clinical transformation, which seriously limits their possibility of clinical application.
[0004] X-rays were originally used for medical imaging diagnosis and X-ray crystallography. X-rays are also a type of radiation that is harmful to the human body, such as ionizing radiation. Existing studies have shown that high doses of X-rays have an impact on life expectancy. Many special occupations, such as orthopedic surgeons, are exposed to large doses of X-rays, which will inevitably affect the health of these people. Summary of the invention
[0005] The present invention provides a hyperbaric oxygen device and its application and method for prolonging life and improving physiological functions. The present invention proves through experiments that effective hyperbaric oxygen therapy can prolong the life of aged mice and mice exposed to X-rays; in addition, effective hyperbaric oxygen therapy can also improve the physiological functions of mice including grip strength, cognition, memory, inflammation level, liver function and oxidative stress level; the present invention provides a new idea for prolonging life and improving physiological functions.
[0006] To achieve this object, the present invention provides the following technical solutions:
[0007] A first aspect of the present invention provides a hyperbaric oxygen device that can prolong life and improve physiological functions. The hyperbaric oxygen device includes a hyperbaric oxygen chamber and a chamber console. The chamber console is set to a pressure of 1.0-3.0 ATA, an oxygen concentration of ≥92%, and a time of 90-180 min.
[0008] Preferably, the cabin console is set to a pressure of 2.0 ATA.
[0009] The second aspect of the present invention improves the application of the hyperbaric oxygen device of the present invention in the preparation of instruments that can prolong life and improve physiological functions.
[0010] Preferably, the life extension includes extending the life of a naturally aging body and extending the life of a patient irradiated with high doses of X-rays.
[0011] Preferably, the improved physiological functions include grip strength, cognition, memory, inflammation level, liver function and oxidative stress level.
[0012] Preferably, the hyperbaric oxygen device comprises a hyperbaric oxygen chamber and a chamber console, and the chamber console is set to a pressure of 1.0-3.0 ATA, an oxygen concentration of ≥92%, and a time of 90-180 min.
[0013] The third aspect of the present invention provides a method for prolonging life and improving physiological function for non-therapeutic purposes, comprising the following steps:
[0014] S1. Place the test object in a hyperbaric oxygen chamber;
[0015] S2. Use high-purity oxygen with an oxygen purity of ≥99.99% to continuously ventilate the hyperbaric oxygen chamber until the replacement oxygen concentration in the hyperbaric oxygen chamber reaches ≥92%;
[0016] S3. Treatment is carried out for 90-180 minutes at a pressure of 1-3.0 ATA. During this period, the chamber is flushed with pure oxygen for 5-10 seconds every 150-300 seconds to ensure that the oxygen content in the chamber is stable.
[0017] Preferably, the method further comprises the following steps: repeating steps S1-S3 at least 6 times a week for a duration of at least 4 weeks, with a gap of at least 24 hours between two consecutive times.
[0018] Preferably, the life extension includes extending the life of a naturally aging body and extending the life of a patient irradiated with high doses of X-rays.
[0019] Preferably, the improved physiological functions include grip strength, cognition, memory, inflammation level, liver function and oxidative stress level.
[0020] Preferably, the method includes: using high-purity oxygen (oxygen purity ≥ 99.99%) for continuous ventilation until the oxygen concentration in the cabin is replaced to ≥ 92%, which takes 20-30 minutes; setting automatic parameters and starting the program: the pressure increase and pressure reduction time are 10 minutes respectively, and 90 minutes of HBO treatment is performed at a pressure of 2.0ATA, and the cabin is flushed with pure oxygen for 5-10 seconds every 150-300 seconds to ensure that the oxygen content in the cabin is stable.
[0021] Compared with the prior art, the beneficial effects and significant progress of the technical solution of the present invention are: the hyperbaric oxygen device provided by the present invention can prolong life and improve physiological functions, providing a more effective, safe and easy-to-implement method to prolong healthy life span, thereby improving the health challenges faced by an aging society. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] In order to more clearly illustrate the technical solution of the present invention, the drawings used in the embodiments of the present invention are briefly introduced below.
[0023] Figure 1 This is a statistical result diagram of the life span of two groups of mice in Example 1;
[0024] Figure 2 is a graph showing the statistical results of the life span of the three groups of mice in Example 2;
[0025] Figure 3 is a graph showing the motor function test results of Example 3;
[0026] Figure 4 is a diagram of the cognitive and memory function test results of Example 3;
[0027] Figure 5 The composition of the immune system and various biochemical index detection results of Example 3 are shown;
[0028] Figure 6 is a graph of liver function test results of Example 3;
[0029] Figure 7 This is a graph showing the oxidative stress level detection results of Example 3. DETAILED DESCRIPTION
[0030] To make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the present invention is further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are weight percentages and weight parts. The experimental materials and reagents used in the following examples can be obtained from commercial channels unless otherwise specified.
[0031] Unless otherwise specified, the technical and scientific terms used herein have the same meaning as commonly understood by ordinary technicians in the technical field to which the application belongs. It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments of the present application.
[0032] In order to more fully understand the present invention, the professional terms in the present invention are explained below.
[0033] The history of hyperbaric oxygen can be traced back to the 17th century, when it was first used to treat hypoxia and ischemic diseases. In the past sixty years, its scope of application has expanded significantly, and a large amount of evidence has been accumulated in modern medicine to prove its safety and non-invasiveness. In addition, the invention and gradual popularization of civilian hyperbaric oxygen chambers are expanding its scope of application and making it more convenient to use. Therefore, as a potential treatment method for prolonging life, hyperbaric oxygen therapy has a good safety foundation and the advantage of being easily translated into clinical practice.
[0034] Oxidative stress (OS) refers to a state of imbalance between oxidation and anti-oxidation in the body, which tends to oxidize, leading to inflammatory infiltration of neutrophils, increased secretion of proteases, and the production of a large number of oxidative intermediates. Oxidative stress is a negative effect produced by free radicals in the body and is considered to be an important factor leading to aging and disease. However, in clinical practice, oxidative stress is closely related to a variety of diseases. It is currently believed that oxidative stress should be used for "systemic evaluation" or "severity and prognosis" evaluation after the cause of the disease has been determined to be oxidative stress.
[0035] The Y-maze test is a behavioral test based on the natural curiosity of animals. The spontaneous alternation behavior in this test is considered to reflect short-term spatial working memory. It uses the test animal's natural instinct to explore new environments. During the experiment, the test animal needs to remember the direction it has explored before each time it switches to explore a new direction. Therefore, the Y-maze test can well reflect the spatial working ability of animals.
[0036] The rotarod test for mice and rats is a commonly used behavioral experiment, mainly used to evaluate the motor coordination and learning and memory abilities of mice. In the experiment, mice need to keep their balance on a rotating rod and remember the direction and speed of the rod's rotation so that they can better adapt to future experiments. This experiment is of great significance for the study of neurological diseases such as Parkinson's disease and Alzheimer's disease.
[0037] Example 1 Hyperbaric oxygen prolongs the life span of a naturally aging organism
[0038] Experimental methods
[0039] 1.1. Construction of a natural aging model in mice
[0040] Unbred C57BL / 6J male mice were housed in an SPF animal room until they were 20 months old.
[0041] 1.2. Hyperbaric oxygen treatment
[0042] Starting from the age of 20 months, the naturally aged model mice were treated with hyperbaric oxygen six times a week for four weeks, with an interval of ≥24 hours between two hyperbaric oxygen treatments. Hyperbaric oxygen treatment was carried out in an animal experiment chamber purchased from Shandong Weifang Huaxin Oxygen Company. The treatment plan included: placing C57BL / 6J mice in a closed ventilation cage and placing them in the chamber; using high-purity oxygen (Shanghai Chloromin Gas Co., Ltd., oxygen purity ≥99.99%) to continuously ventilate the animal experiment chamber until the oxygen concentration in the chamber was replaced to ≥92%, which took 20-30 minutes; setting automatic parameters and starting the program: the pressure increase and decompression time were 10 minutes respectively, and HBO treatment was performed for 90 minutes at a pressure of 2.0ATA, and the chamber was flushed with pure oxygen for 5-10 seconds every 150-300 seconds to ensure the stability of the oxygen content in the chamber.
[0043] 1.3 Experimental Grouping
[0044] In this embodiment, two groups were set up: the OLD+HBO group consisted of naturally aged mice that received hyperbaric oxygen treatment, and the hyperbaric oxygen treatment was completed according to the method in step 1.2; the OLD group consisted of naturally aged mice that received sham treatment, and they were placed in the same ventilation cage and in the animal experimental chamber for the same period of time, but no ventilation treatment was performed.
[0045] 1.4. After the last hyperbaric oxygen treatment, the aged mice continued to be kept in the animal room, and the natural death time of each mouse was monitored and recorded until all mice died.
[0046] Experimental Results
[0047] The results are as follows Figure 1As shown, the total lifespan (Figure a) and post-treatment survival time (Figure b) of old mice were significantly prolonged after hyperbaric oxygen treatment. Compared with the 717-day survival of the control group, the HBO-treated animals had a median survival of 768 days, an increase of 7.1%. At the same time, the median survival time after treatment was extended from 87 days to 138 days, an increase of 58.6%.
[0048] Example 2 Hyperbaric oxygen prolongs the life span of patients exposed to high doses of X-rays
[0049] Experimental methods
[0050] 2.1. Construction of high-dose X-ray irradiation model
[0051] We used a biological X-ray irradiator (CIX3, UK) to perform a single irradiation of 6-week-old C57BL / 6J male mice at a dose of 5 Gy, and then continued to keep them in the animal room for 8 weeks after irradiation.
[0052] 2.2 Experimental Grouping
[0053] Then they were randomly divided into three groups, each with 15 mice. One group received the hyperbaric oxygen treatment as described in step 1.2 of Example 1 (RAD+HBO group), and the other group received sham treatment, that is, they were placed in the same ventilation cage and placed in the animal experimental chamber for the same time, but without ventilation treatment (RAD group). The Naive group consisted of C57BL / 6J male mice that were not irradiated and were raised normally.
[0054] 2.3. After the last hyperbaric oxygen treatment, the aged mice continued to be kept in the animal room, and the natural death time of each mouse was monitored and recorded until all mice died.
[0055] Experimental Results
[0056] Irradiated mice have the characteristic of accelerated aging and significantly shortened lifespan, but it has also been reported that some mice have extremely long lifespans. Therefore, we tested the survival time of irradiated aged mice after hyperbaric oxygen treatment, but did not observe the end of lifespan of all mice. Figure 2 As shown, irradiation caused a significant shortening of the lifespan of mice compared with the Naive group, but was partially reversed by HBO treatment. Specifically, the median lifespan was extended from 229 days to 254 days in HBO-treated irradiated mice, an increase of 10.9% (Figure a), while the median survival time after treatment was extended from 135 days to 160 days, an increase of 18.5% (Figure b). Consistent with the results of naturally aged mice, the generally shorter survival of our irradiated mice compared with the reported results is due to the negative effects caused by the stress of daily transfer to the cabin, which does not affect the comparability of the results between the two groups.
[0057] Example 3
[0058] In order to comprehensively evaluate the effect of hyperbaric oxygen on the healthy life span of mice, in this example, three groups of mice, including naturally aged mice treated with or without hyperbaric oxygen and young control mice, were tested for physiological functions.
[0059] Three groups were set up in this example: the OLD+HBO group was composed of naturally aged mice that received hyperbaric oxygen treatment, and the hyperbaric oxygen treatment was performed according to the method in step 1.2; the OLD group was composed of naturally aged mice that received sham treatment, and they were placed in the same ventilation cage and in the animal experimental chamber for the same time, but without ventilation treatment; the YNG group was composed of young mice that received sham treatment, and they were placed in the same ventilation cage and in the animal experimental chamber for the same time, but without ventilation treatment.
[0060] Motor function test
[0061] The grip strength and rotarod test of the three groups of mice were tested. The two tests were tested using relevant instruments respectively. The experimental methods were conventional methods in the prior art.
[0062] The results are as follows Figure 3 As shown, hyperbaric oxygen significantly improved the declined motor function of aged mice, as manifested by improved grip strength (Figure a) and improved rotarod performance (Figure b).
[0063] Cognitive and memory tests
[0064] The three groups of mice were tested in novel object recognition test and Y-maze test. The experimental method is conventional method in the prior art.
[0065] The results are as follows Figure 4 As shown, in the new object recognition test (Figure a) and the Y-maze (Figures b, c) experiments, the performance of the elderly mice receiving hyperbaric oxygen was improved compared with the control elderly mice, and the movement trajectory characteristics in the Y-maze were more similar to those of the young group, indicating that their cognitive and memory functions were improved.
[0066] Immune system composition and various biochemical indicators
[0067] The effects of hyperbaric oxygen on the circulatory system of mice were examined, mainly the composition of the immune system and various biochemical indicators. Aging is accompanied by inflammatory aging, which manifests as a chronic low-grade inflammatory state of the body.
[0068] The results are as follows Figure 5As shown, hyperbaric oxygen has a significant effect on the number and composition of mouse leukocytes, which is manifested in the total number of leukocytes and the proportion of various leukocytes tending to the phenotype of young mice (Figure a, b). When we examined the changes in the absolute number of various leukocytes, except for the number of lymphocytes reflecting adaptive immune function, which did not change significantly, the number of various granulocytes and monocytes recovered to a certain extent (Figure a).
[0069] Liver function tests
[0070] The CRE levels, AST levels and AST / ALT ratios of the three groups of mice were detected to evaluate the liver functions of the three groups of mice.
[0071] The results are as follows Figure 6 As shown, biochemical tests showed that the serum creatinine (CRE) level decreased after hyperbaric oxygen treatment, indicating that renal dysfunction had recovered to a certain extent. The AST level and AST / ALT ratio in aged mice returned to normal after hyperbaric oxygen treatment, indicating that the age-related decline in liver function can be reversed by hyperbaric oxygen.
[0072] Oxidative stress level detection
[0073] Considering the physical properties of HBO, it was evaluated whether oxidative stress levels would be adversely altered after HBO treatment.
[0074] The results are as follows Figure 7 As shown, the systemic oxidative stress level of aged mice decreased after HBO treatment, as reflected by ELISA detection of serum 8-OHdG and HNE adducts.
[0075] The results of this example further illustrate that the effect of hyperbaric oxygen on elderly mice is not only to prolong the life span, but also to improve the overall physiological function, thereby promoting the extension of the overall healthy life span.
[0076] The applicant declares that, in the description of the above specification:
[0077] The descriptions of the terms "this embodiment", "an embodiment of the present invention", "as shown in...", "further", "a further improved technical sub-scheme", etc., mean that the specific features, structures, materials or characteristics described in the embodiment or example are included in at least one embodiment or example of the present invention; in this specification, the schematic expressions of the above terms are not necessarily for the same embodiment or example, and the specific features, structures, materials or characteristics described may be combined or combined in a suitable manner in any one or more embodiments or examples; in addition, a person of ordinary skill in the art may combine or combine the different embodiments or examples and the features of the different embodiments or examples described in this specification without causing any contradiction.
[0078] Finally, it should be noted that:
[0079] The above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them.
[0080] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein, and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention. The non-essential improvements, adjustments or replacements made by those skilled in the art based on the contents of this specification are all within the scope of protection required by the present invention.
Claims
1. A hyperbaric oxygen device capable of prolonging life and improving physiological functions, characterized in that: The hyperbaric oxygen device comprises a hyperbaric oxygen chamber and a chamber console, wherein the chamber console is set to have a pressure of 1.0-3.0 ATA, an oxygen concentration of ≥92%, and a time of 90-180 min.
2. The hyperbaric oxygen device capable of prolonging life and improving physiological functions as claimed in claim 1, characterized in that: The cabin console was set to a pressure of 2.0 ATA.
3. Use of the hyperbaric oxygen device according to claim 1 or 2 in the preparation of an instrument capable of prolonging life and improving physiological function.
4. The use according to claim 3, characterized in that The life extension includes extending the life of a naturally aging body and extending the life of a patient irradiated with a high dose of X-rays.
5. The use according to claim 3, characterized in that The improved physiological functions include grip strength, cognition, memory, inflammation levels, liver function and oxidative stress levels.
6. The use according to claim 3, characterized in that The hyperbaric oxygen device comprises a hyperbaric oxygen chamber and a chamber console, wherein the chamber console is set to have a pressure of 1.0-3.0 ATA, an oxygen concentration of ≥92%, and a time of 90-180 min.
7. A method for prolonging life and improving physiological function for non-therapeutic purposes, characterized in that: The following steps are involved: S1. Place the test object in a hyperbaric oxygen chamber; S2. Use high-purity oxygen with an oxygen purity of ≥99.99% to continuously ventilate the hyperbaric oxygen chamber until the replacement oxygen concentration in the hyperbaric oxygen chamber reaches ≥92%; S3. Treatment is carried out for 90-180 minutes at a pressure of 1-3.0 ATA. During this period, the chamber is flushed with pure oxygen for 5-10 seconds every 150-300 seconds to ensure that the oxygen content in the chamber is stable.
8. The method for prolonging life and improving physiological function for non-therapeutic purposes according to claim 7, characterized in that: The following steps are also included: Repeat steps S1-S3 at least 6 times a week for at least 4 weeks, with a gap of at least 24 hours between two consecutive steps.
9. The method for prolonging life and improving physiological function for non-therapeutic purposes according to claim 7, characterized in that: The life extension includes extending the life of a naturally aging body and extending the life of a patient irradiated with a high dose of X-rays.
10. The method for prolonging life and improving physiological function for non-therapeutic purposes according to claim 7, characterized in that: The improved physiological functions include grip strength, cognition, memory, inflammation levels, liver function and oxidative stress levels.