Anti-saccharification emulsion as well as preparation method and application thereof

Abstract

The invention discloses an anti-saccharification emulsion and a preparation method and application thereof, and relates to the technical field of cosmetics, the anti-saccharification emulsion comprises the following components: polyol, skin moistening oil, an anti-saccharification composition, an emulsifier, ethylene glycol palmitate, PPG-26 oleate, polydimethylsiloxane, a preservative and deionized water; the anti-saccharification composition is prepared by the following steps: coating fullerene with carnosine modified cyclodextrin to obtain an inclusion compound, and mixing the inclusion compound with chitosan and bentonite to obtain the inclusion compound loaded in situ on chitosan-bentonite hydrogel, namely the anti-saccharification composition. The stable ternary co-assembled supramolecular hydrogel is formed, and the system is high in water content and good in biocompatibility and has multiple biological activities of saccharification resistance and oxidation resistance.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to an anti-glycation emulsion and a preparation method and application thereof. Background Art

[0002] As people age, their metabolism slows down, causing the sugars accumulated in the body to be unable to be metabolized in time. The excess sugars are bonded to the amino acid groups of proteins through non-enzymatic glycosylation reactions to form glycated proteins, i.e., highly glycated end products of proteins. Collagen and elastin serve as the scaffolds of the skin. Once combined with sugars, the plump and elastic proteins themselves will harden, break, and become damaged, causing the skin to collapse, lose elasticity, and lose firmness. At the same time, as glycated proteins accumulate in the skin, wrinkles will slowly appear, and the skin will gradually become dull. Therefore, the development and preparation of anti-sugar cosmetics is the focus and hotspot of current research. Currently, there are few anti-sugar cosmetics on the market, and they generally have the disadvantages of single efficacy and slow effect, which cannot meet the needs of people to comprehensively improve skin problems in their daily lives.

[0003] Fullerene has a good ability to absorb free radicals, so it is considered to have antioxidant and anti-glycation effects. However, due to its poor water solubility, its application in the body is very limited. In order to solve this problem, scientists have made many efforts to improve the biological activity of fullerene. Studies have shown that the introduction of cyclodextrin can effectively increase the solubility of fullerene; carnosine can react with sugar before sugar erodes the skin, replacing the body's protein to react with sugar, thereby helping to protect the protein from being glycosylated and achieving an anti-glycation effect; it is also an important intracellular antioxidant that can remove reactive oxygen species (ROS) and prevent peroxidation of cell membrane fatty acids during oxidative stress. Summary of the invention

[0004] The purpose of the present invention is to provide an anti-glycation emulsion and a preparation method and application thereof, so as to solve the problems raised in the background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: an anti-glycation emulsion and a preparation method and application thereof.

[0006] An anti-glycation emulsion, comprising the following components in parts by weight: 4-8 parts of polyol, 6-12 parts of moisturizing oil, 1-2 parts of anti-glycation composition, 3-5 parts of emulsifier, 3-5 parts of ethylene glycol palmitate, 2-4 parts of PPG-26 oleate, 0.5-2 parts of polydimethylsiloxane, 0.5-1 parts of preservative and 95-100 parts of deionized water;

[0007] The anti-glycation composition is an inclusion compound obtained by coating fullerene with cyclodextrin modified with carnosine, and then the inclusion compound is mixed with chitosan and bentonite to form an inclusion compound loaded in situ by chitosan-bentonite hydrogel, namely the anti-glycation composition.

[0008] Furthermore, the preparation method of the anti-glycation composition is:

[0009] S1, dissolving d-carnosine ethyl ester and iodinated cyclodextrin in DMF, reacting for 24 hours under a nitrogen atmosphere at 60° C., and obtaining carnosine-modified cyclodextrin after purification by ion exchange chromatography;

[0010] S2, grinding the fullerene in a mortar, then mixing it with the carnosine-modified cyclodextrin, and continuing to grind, mixing the ground mixture with distilled water, ultrasonicating for 6 hours under an inert gas atmosphere, filtering the resulting solution with a disposable needle filter membrane with a pore size of 0.8 μm, and freeze-drying the filtrate under vacuum conditions to obtain a fullerene inclusion compound;

[0011] S3. Dissolve the fullerene inclusion compound in distilled water, and add the mixture to the chitosan-bentonite hydrogel to obtain an anti-glycation composition.

[0012] Furthermore, in step S1, the dosage ratio of d-carnosine ethyl ester, iodinated cyclodextrin and DMF is 0.05-0.08 g: 0.05-0.08 g: 40 mL.

[0013] Furthermore, in step S2, the dosage ratio of fullerene, carnosine-modified cyclodextrin and distilled water is 1-5 mg: 20-30 mg: 10-30 mL.

[0014] Furthermore, the preparation method of the chitosan-bentonite hydrogel in step S3 is:

[0015] Dissolve bentonite in distilled water, stir thoroughly, and take the upper suspension; dissolve chitosan in a diluted acetic acid aqueous solution with a mass fraction of 1%; add the upper layer of the bentonite suspension; mix and stir the two overnight; add a sodium hydroxide aqueous solution with a molar concentration of 0.5M to the chitosan-bentonite mixture, and stir overnight; filter to obtain a chitosan-bentonite gel, wash with distilled water, dialyze with distilled water for 5-7 days, and filter the dialyzed solution to obtain a chitosan-bentonite hydrogel.

[0016] Furthermore, the concentration of the bentonite suspension and the dilute acetic acid aqueous solution of chitosan is 15 g / L; the volume ratio of the upper layer of the bentonite suspension, the dilute acetic acid aqueous solution and the sodium hydroxide aqueous solution is 2:3:3;

[0017] Furthermore, in step S3, the dosage ratio of the fullerene inclusion compound, the chitosan-bentonite hydrogel and the distilled water is 10-15 mg: 10-15 g: 1-2 mL.

[0018] Furthermore, the polyol is 1,3-propylene glycol, glycerol, butylene glycol or 1,2-pentanediol; the moisturizing oil is white meadowfoam seed oil, avocado oil, macadamia oil or sweet almond oil; the emulsifier is PEG-30 dipolyhydroxystearate or sorbitan sesquioleate; and the preservative is phenoxyethanol or 1,2-pentanediol.

[0019] A method for preparing an anti-glycation emulsion comprises the following steps: mixing a moisturizing oil, an emulsifier, ethylene glycol palmitate, PPG-26 oleate and polydimethylsiloxane by weight, heating the mixture to 80-90°C, stirring and dissolving the mixture to obtain an oil phase; mixing a polyol, a preservative and deionized water, heating the mixture to 80-90°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 3-5 minutes, cooling the mixture to 50-60°C, adding an anti-glycation composition and homogenizing the mixture for 3-5 minutes to obtain an anti-glycation skin care emulsion.

[0020] An application of an anti-glycation lotion: it can be directly applied on the skin surface.

[0021] Compared with the prior art, the present invention has the following beneficial effects: (1) D-carnosine (D-Car) is a naturally occurring enantiomer of a dipeptide, has the same properties as L-carnosine (L-Car), and is not easily hydrolyzed by carnosinase. When D-carnosine is connected to cyclodextrin, D-carnosine can enter the hydrophobic cavity of cyclodextrin. Such host-guest complex can better inhibit the hydrolysis of D-carnosine, maintain the physiological activity of D-carnosine, and improve its antioxidant and anti-glycation efficiency; (2) Carnosine-modified cyclodextrin can improve the water solubility, stability and bioavailability of fullerene. Carnosine itself can protect proteins from being glycosylated and has good anti-glycation performance. In addition, the original biological function of fullerene is retained. The hydrogel is prepared by combining the hydrogel with chitosan and bentonite to form a stable ternary co-assembled supramolecular hydrogel. The system has high water content, good biocompatibility and multiple biological activities of anti-glycation and anti-oxidation. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0023] The present invention provides a technical solution: a method for preparing an anti-glycation emulsion.

[0024] Preparation method of anti-glycation composition:

[0025] S1, dissolving 0.6 g of D-carnosine ethyl ester and 0.6 g of iodinated cyclodextrin in 40 mL of DMF, reacting for 24 h under a nitrogen atmosphere at 60° C., and obtaining carnosine-modified cyclodextrin after purification by ion exchange chromatography;

[0026] S2, 1 mg of fullerene was fully ground with a mortar, then mixed with 20 mg of carnosine-modified cyclodextrin, and the ground mixture was mixed with 10 mL of distilled water, and ultrasonicated for 6 h under an inert gas atmosphere, and the resulting solution was filtered with a disposable needle filter membrane with a pore size of 0.8 μm, and the filtrate was freeze-dried under vacuum conditions to obtain a fullerene inclusion compound;

[0027] S3. Dissolve 10 mg of the fullerene inclusion compound in 1 mL of distilled water, and add the mixture to 10 g of chitosan-bentonite hydrogel to obtain an anti-glycation composition.

[0028] Preparation method of chitosan-bentonite hydrogel:

[0029] Dissolve 450 mg of bentonite in 30 mL of distilled water, stir thoroughly, and take the upper suspension; dissolve 300 mg of chitosan in 20 mL of dilute acetic acid aqueous solution with a mass fraction of 1%; add 20 mL of the upper bentonite suspension; mix and stir the two overnight; add 30 mL of sodium hydroxide aqueous solution with a molar concentration of 0.5 M to the chitosan-bentonite mixture, stir overnight; filter to obtain chitosan-bentonite gel, wash with distilled water, dialyze with distilled water for 5 days, and filter the dialyzed solution to obtain chitosan-bentonite hydrogel.

[0030] Embodiment 1:

[0031] A method for preparing an anti-glycation emulsion comprises the following steps of: mixing 8 parts of meadowfoam seed oil, 4 parts of PEG-30 dipolyhydroxystearate, 4 parts of ethylene glycol palmitate, 3 parts of PPG-26 oleate and 1 part of polydimethylsiloxane by weight, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an oil phase; mixing 6 parts of 1,3-propylene glycol, 0.6 parts of phenoxyethanol and 95 parts of deionized water, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 3 minutes, cooling the mixture to 50°C, adding 1.5 parts of an anti-glycation composition and homogenizing the mixture for 3 minutes to obtain an anti-glycation skin care emulsion.

[0032] Embodiment 2:

[0033] A method for preparing an anti-glycation emulsion comprises the following steps: mixing 6 parts of avocado oil, 3 parts of sorbitan sesquioleate, 3 parts of ethylene glycol palmitate, 2 parts of PPG-26 oleate and 0.5 parts of polydimethylsiloxane by weight, heating the mixture to 80°C, stirring and dissolving the mixture to obtain an oil phase; mixing 4 parts of glycerol, 0.5 parts of ethylhexylglycerol and 95 parts of deionized water, heating the mixture to 80°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 3 minutes, cooling the mixture to 50°C, adding 1 part of an anti-glycation composition and homogenizing the mixture for 3 minutes to obtain an anti-glycation skin care emulsion.

[0034] Embodiment 3:

[0035] A preparation method of an anti-glycation emulsion: by weight, 10 parts of macadamia nut oil, 5 parts of PEG-30 dipolyhydroxystearate, 5 parts of ethylene glycol palmitate, 4 parts of PPG-26 oleate and 3 parts of polydimethylsiloxane are mixed, the temperature is raised to 88°C, and the mixture is stirred and dissolved to obtain an oil phase; 7 parts of butylene glycol, 0.8 parts of phenoxyethanol and 98 parts of deionized water are mixed, the temperature is raised to 88°C, and the mixture is stirred and dissolved to obtain an aqueous phase; the aqueous phase is added to the oil phase, homogenized for 4 minutes, the temperature is lowered to 58°C, and 1.8 parts of an anti-glycation composition are added, and the mixture is homogenized for 4 minutes to obtain an anti-glycation skin care emulsion.

[0036] Embodiment 4:

[0037] A method for preparing an anti-glycation emulsion comprises the following steps of: mixing 12 parts of sweet almond oil, 5 parts of sorbitan sesquioleate, 5 parts of ethylene glycol palmitate, 4 parts of PPG-26 oleate and 2 parts of polydimethylsiloxane by weight, heating the mixture to 90°C, stirring and dissolving the mixture to obtain an oil phase; mixing 8 parts of 1,2-pentanediol, 1 part of 1,2-pentanediol and 100 parts of deionized water, heating the mixture to 90°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 5 minutes, cooling the mixture to 60°C, adding 2 parts of an anti-glycation composition and homogenizing the mixture for 5 minutes to obtain an anti-glycation skin care emulsion.

[0038] Comparative Example 1:

[0039] The preparation method of the anti-glycation composition in this comparative example is:

[0040] 0.6 g of D-carnosine ethyl ester and 0.6 g of iodinated cyclodextrin were dissolved in 40 mL of DMF, reacted at 60° C. in a nitrogen atmosphere for 24 hours, and purified by ion exchange chromatography to obtain carnosine-modified cyclodextrin as an anti-glycation composition.

[0041] A method for preparing an anti-glycation emulsion comprises the following steps of: mixing 8 parts of meadowfoam seed oil, 4 parts of PEG-30 dipolyhydroxystearate, 4 parts of ethylene glycol palmitate, 3 parts of PPG-26 oleate and 1 part of polydimethylsiloxane by weight, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an oil phase; mixing 6 parts of 1,3-propylene glycol, 0.6 parts of phenoxyethanol and 95 parts of deionized water, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 3 minutes, cooling the mixture to 50°C, adding 1.5 parts of an anti-glycation composition and homogenizing the mixture for 3 minutes to obtain an anti-glycation skin care emulsion.

[0042] Comparative Example 2:

[0043] The preparation method of the anti-glycation composition in this comparative example is:

[0044] S1. Grind 1 mg of fullerene in a mortar, mix with 20 mg of hydroxypropyl cyclodextrin, continue grinding, mix the ground mixture with 10 mL of distilled water, ultrasonicate for 6 h under an inert gas atmosphere, filter the resulting solution with a disposable needle filter membrane with a pore size of 0.8 μm, and freeze-dry the filtrate under vacuum conditions to obtain a fullerene inclusion compound;

[0045] S3. Dissolve 10 mg of the fullerene inclusion compound in 1 mL of distilled water, and add the mixture to 10 g of chitosan-bentonite hydrogel to obtain an anti-glycation composition.

[0046] A method for preparing an anti-glycation emulsion comprises the following steps of: mixing 8 parts of meadowfoam seed oil, 4 parts of PEG-30 dipolyhydroxystearate, 4 parts of ethylene glycol palmitate, 3 parts of PPG-26 oleate and 1 part of polydimethylsiloxane by weight, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an oil phase; mixing 6 parts of 1,3-propylene glycol, 0.6 parts of phenoxyethanol and 95 parts of deionized water, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 3 minutes, cooling the mixture to 50°C, adding 1.5 parts of an anti-glycation composition and homogenizing the mixture for 3 minutes to obtain an anti-glycation skin care emulsion.

[0047] Comparative Example 3:

[0048] The anti-glycation composition in this comparative example is D-carnosine.

[0049] A method for preparing an anti-glycation emulsion comprises the following steps of: mixing 8 parts of meadowfoam seed oil, 4 parts of PEG-30 dipolyhydroxystearate, 4 parts of ethylene glycol palmitate, 3 parts of PPG-26 oleate and 1 part of polydimethylsiloxane by weight, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an oil phase; mixing 6 parts of 1,3-propylene glycol, 0.6 parts of phenoxyethanol and 95 parts of deionized water, heating the mixture to 85°C, stirring and dissolving the mixture to obtain an aqueous phase; adding the aqueous phase to the oil phase, homogenizing the mixture for 3 minutes, cooling the mixture to 50°C, adding 1.5 parts of an anti-glycation composition and homogenizing the mixture for 3 minutes to obtain an anti-glycation skin care emulsion.

[0050] The samples of each embodiment and comparative example were tested for their anti-glycation ability;

[0051] Glycation reaction is an important mechanism of skin aging, which occurs between reducing sugars and matrix proteins in the skin. Non-enzymatic glycosylation is a series of complex non-enzymatic reactions. Proteins and glucose react non-enzymatically in the body to form Schiff bases and early glycosylation products, which then undergo oxidation, rearrangement, cross-linking and other processes to form irreversible non-enzymatic glycosylation end products (AGEs). By studying non-enzymatic glycosylation reactions, the ability of the skin composition to resist glycation can be reflected. AGEs can spontaneously fluoresce, and the fluorescence intensity can be detected at a specific wavelength to obtain the anti-glycation effect of the skin composition.

[0052] The test method is as follows: (1) Preparation of bovine serum albumin-glucose solution: under sterile conditions, add 5.00 mL of 20.00 g / L bovine serum albumin solution and 0.50 mol / L glucose solution sterilized with 0.2 μm sterilization film to a sterile cell culture bottle, add 10.00 mL of 0.20 mol / L (pH=7.4) phosphate buffer containing 1% NaN3, and react at 60°C in the dark for 40 hours to obtain a bovine serum albumin-glucose saccharification solution; (2) Sampling: accurately transfer 0.5 mL of the samples obtained in Examples 1-4 and Comparative Examples 1-3 respectively, mix them with 0.5 mL of bovine serum albumin-glucose solution, and react in the dark for 0.8 hours; (3) Detection of AGEs: The fluorescence derived from AGEs can be detected by an ELISA reader under the emission wavelength conditions of 370 nm / 440 nm, and its inhibition rate is calculated. The calculation formula is as follows:

[0053] AGEs inhibition rate (%) = [l-(AB) / (CD)] × 100%;

[0054] Wherein: A is the fluorescence intensity of the sample obtained in Example 1-4 or Comparative Example 1-3 added in step (2) in a bovine serum albumin-glucose solution; B is the fluorescence intensity of the sample obtained in Example 1-4 or Comparative Example 1-3 added in step (2) in 0.5 mL of ultrapure water solution; C is the fluorescence intensity of a single bovine serum albumin-glucose solution (i.e., the fluorescence intensity of the bovine serum albumin-glucose solution obtained by replacing an equal amount of the sample obtained in Example 1-4 or Comparative Example 1-3 in step (2) with ultrapure water); D is the fluorescence intensity of 1 mL of ultrapure water solution;

[0055] The samples of each embodiment and comparative example were tested for their antioxidant capacity;

[0056] 1,1-Diphenyl-2-trinitrobenzene phosphine (DPPH) has a single electron and strong absorption at 517nm. Its anhydrous ethanol solution is purple. When there is a free radical scavenger, its color gradually disappears due to pairing with its single electron. Its fading degree is quantitatively related to the number of electrons it accepts, so it can be quickly quantitatively analyzed by a spectrophotometer. The ability to scavenge DPPH free radicals can reflect the antioxidant capacity of a substance to a certain extent. The greater the free radical scavenging rate, the stronger the antioxidant capacity.

[0057] Therefore, the antioxidant effect of the skin composition can be judged by studying its ability to scavenge DPPH free radicals. The test method is as follows: (1) Preparation of DPPH-ethanol solution: Weigh 12 mg of 1,1-diphenyl-2-trinitrobenzene, dissolve in anhydrous ethanol, add anhydrous ethanol to make up to 250 mL, and use it immediately; (2) Sampling test: Take 1 mL of the composition of Examples 1-4 and Comparative Examples 1-3 and 1 mL of DPPH-ethanol solution, add them to a 5 mL brown volumetric flask, add 3 mL of anhydrous ethanol, shake well, react in the dark for 30 minutes, measure the absorbance at a wavelength of 517 nm with a cuvette, and calculate its free radical scavenging rate, the calculation formula is as follows;

[0058] Free radical scavenging rate (%) = [l-(AB) / (CD)] × 100%;

[0059] Wherein, A is the absorbance at 517 nm of the sample of Example 1-4 or Comparative Example 1-3 added in step (2) after reacting with 1 mL of DPPH-ethanol solution; B is the absorbance at 517 nm of the sample of Example 1-4 or Comparative Example 1-3 added in step (2) in 1 mL of ultrapure water solution; C is the absorbance at 517 nm of a single DPPH-ethanol solution (i.e., the absorbance at 517 nm of the DPPH-ethanol solution obtained by replacing 1 mL of the sample solution of Example 1-4 or Comparative Example 1-3 in step (2) with an equal amount of ultrapure water); D is the absorbance at 517 nm of a solution obtained by mixing 2 mL of ultrapure water and 3 mL of anhydrous ethanol.

[0060] The test results are shown in Table 1;

[0061] Table 1: Experimental results of various embodiments and comparative examples

[0062] sample AGEs inhibition rate (%) Free radical scavenging rate (%) Example 1 75.6 88.5 Example 2 74.9 87.7 Example 3 73.4 86.6 Example 4 74.8 87.5 Comparative Example 1 60.4 70.1 Comparative Example 2 58.6 68.4 Comparative Example 3 50.9 56.8

[0063] Analysis of the experimental data shows that in each embodiment, the cyclodextrin modified with carnosine forms an inclusion complex with fullerene, which improves the water solubility and bioavailability of fullerene, thereby improving the anti-glycation and antioxidant properties of the system. In addition, carnosine is coated in the hydrophobic cavity of cyclodextrin, which reduces the possibility of its hydrolysis, can better protect the protein, and forms a ternary co-assembled supramolecular hydrogel with fullerene and chitosan-bentonite to improve the anti-glycation and antioxidant properties of the system; in the comparative example, the anti-glycation components are cyclodextrin modified with carnosine, a fullerene-chitosan-bentonite system without carnosine, and a simple carnosine molecule. Although it also has a certain anti-glycation ability, compared with the embodiment, the simple carnosine molecule is easily hydrolyzed and cannot exert its anti-glycation and antioxidant effects. The bioavailability and biocompatibility of the cyclodextrin modified with carnosine are not high, and the anti-glycation and antioxidant activity of the fullerene-chitosan-bentonite system without carnosine is poorer than that of the embodiment.

[0064] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An anti-glycation emulsion, Features: The composition comprises the following components in parts by weight: 4-8 parts of polyol, 6-12 parts of emollient oil, 1-2 parts of anti-glycation composition, 3-5 parts of emulsifier, 3-5 parts of ethylene glycol palmitate, 2-4 parts of PPG-26 oleate, 0.5-2 parts of polydimethylsiloxane, 0.5-1 parts of preservative and 95-100 parts of deionized water; The anti-glycation composition is an inclusion compound obtained by coating fullerene with cyclodextrin modified with carnosine, and then the inclusion compound is mixed with chitosan and bentonite to form an inclusion compound loaded in situ by chitosan-bentonite hydrogel, namely the anti-glycation composition.

2. An anti-glycation emulsion according to claim 1, Features: The preparation method of the anti-glycation composition is: S1, dissolving d-carnosine ethyl ester and iodinated cyclodextrin in DMF, reacting for 24 hours under a nitrogen atmosphere at 60° C., and obtaining carnosine-modified cyclodextrin after purification by ion exchange chromatography; S2, grinding the fullerene in a mortar, then mixing it with the carnosine-modified cyclodextrin, and continuing to grind, mixing the ground mixture with distilled water, ultrasonicating for 6 hours under an inert gas atmosphere, filtering the resulting solution with a disposable needle filter membrane with a pore size of 0.8 μm, and freeze-drying the filtrate under vacuum conditions to obtain a fullerene inclusion compound; S3. Dissolve the fullerene inclusion compound in distilled water, and add the mixture to the chitosan-bentonite hydrogel to obtain an anti-glycation composition.

3. An anti-glycation emulsion according to claim 2, Features: In the step S1, the dosage ratio of d-carnosine ethyl ester, iodinated cyclodextrin and DMF is 0.05-0.08 g: 0.05-0.08 g: 40 mL.

4. The anti-glycation emulsion according to claim 2, Features: In the step S2, the dosage ratio of fullerene, carnosine-modified cyclodextrin and distilled water is 1-5 mg: 20-30 mg: 10-30 mL.

5. The anti-glycation emulsion according to claim 2, Features: The preparation method of chitosan-bentonite hydrogel in step S3 is: Dissolve bentonite in distilled water, stir thoroughly, and take the upper suspension; dissolve chitosan in a diluted acetic acid aqueous solution with a mass fraction of 1%; add the upper layer of the bentonite suspension; mix and stir the two overnight; add a sodium hydroxide aqueous solution with a molar concentration of 0.5M to the chitosan-bentonite mixture, and stir overnight; filter to obtain a chitosan-bentonite gel, wash with distilled water, dialyze with distilled water for 5-7 days, and filter the dialyzed solution to obtain a chitosan-bentonite hydrogel.

6. The anti-glycation emulsion according to claim 5, Features: The concentration of the dilute acetic acid aqueous solution of the bentonite suspension and chitosan is 15 g / L; the volume ratio of the upper layer of the bentonite suspension, the dilute acetic acid aqueous solution and the sodium hydroxide aqueous solution is 2:3:

3.

7. The anti-glycation emulsion according to claim 2, Features: In the step S3, the dosage ratio of the fullerene inclusion compound, the chitosan-bentonite hydrogel and the distilled water is 10-15 mg: 10-15 g: 1-2 mL.

8. The anti-glycation emulsion according to claim 1, Features: The polyol is 1,3-propylene glycol, glycerin, butylene glycol or 1,2-pentanediol; the moisturizing oil is white meadowfoam seed oil, avocado oil, macadamia oil or sweet almond oil; the emulsifier is PEG-30 dipolyhydroxystearate or sorbitan sesquioleate; and the preservative is phenoxyethanol or 1,2-pentanediol.

9. A method for preparing an anti-glycation emulsion according to any one of claims 1 to 8, Features: The invention discloses a method for preparing an anti-glycation skin care lotion. The method comprises mixing a moisturizing oil, an emulsifier, ethylene glycol palmitate, PPG-26 oleate and polydimethylsiloxane by weight, heating the mixture to 80-90°C, stirring and dissolving the mixture to obtain an oil phase. The method comprises mixing a polyol, a preservative and deionized water, heating the mixture to 80-90°C, stirring and dissolving the mixture to obtain an aqueous phase. The aqueous phase is added to the oil phase, homogenized for 3-5 minutes, cooled to 50-60°C, and then adding an anti-glycation composition and homogenizing the mixture for 3-5 minutes to obtain an anti-glycation skin care lotion.

10. Use of an anti-glycation emulsion according to any one of claims 1 to 8, Features: The anti-glycation emulsion can be directly applied to the skin surface.