Amino acid liquid fertilizer capable of enhancing stress resistance of crops and preparation method of amino acid liquid fertilizer

Abstract

The invention discloses an amino acid liquid fertilizer capable of enhancing crop stress resistance and a preparation method thereof, and belongs to the technical field of compound fertilizer preparation. The amino acid liquid fertilizer capable of enhancing crop stress resistance is prepared from the following components in parts by weight: 62 to 84 parts of xylitol, 30 to 43 parts of glucose, 30 to 42 parts of polypeptide, 8 to 12 parts of chelated trace elements, 10 to 15 parts of urea, 10 to 18 parts of composite fiber composite bacteria, 6 to 12 parts of amino acid derivatives and 80 to 100 parts of deionized water, the composite fiber composite bacteria are prepared from polyacrylonitrile, polyvinylpyrrolidone, polyvinylidene fluoride and beneficial bacteria, and the amino acid derivative is prepared from cassia twig, L-alanine and potassium hydroxide. The liquid fertilizer prepared by the method has the effects of promoting plant growth, increasing crop stress resistance and improving the water-retaining property of soil.

Description

Technical Field

[0001] The invention belongs to the technical field of compound fertilizer preparation, and particularly relates to an amino acid liquid fertilizer capable of enhancing crop stress resistance and a preparation method thereof. Background Art

[0002] As global climate change intensifies, the crop growth environment is becoming increasingly complex and changeable. Environmental pressures such as drought, floods, salinity, pests and diseases pose severe challenges to crop growth. These adverse factors not only affect the normal growth cycle of crops, but may also lead to a significant drop in yields or even cause crop death, causing huge losses to agricultural production. Therefore, it is particularly urgent to develop and apply new fertilizers that can enhance crop resistance.

[0003] The long-term and large-scale application of traditional inorganic fertilizers has gradually exposed many drawbacks. Although inorganic fertilizers can quickly increase crop yields in a short period of time, their low utilization rate, easy flow, and easy pollution of soil and water sources are becoming increasingly prominent. The utilization rate of phosphorus and potassium fertilizers in the current season is only about 20%, and the utilization rate of nitrogen fertilizers in the current season is only about 35%. This not only causes waste of resources and economic losses, but also aggravates environmental pollution problems. In addition, long-term reliance on inorganic fertilizers can easily lead to soil structure damage and decreased fertility, thereby reducing crop resistance and quality. Amino acid liquid fertilizer, as an organic fertilizer, has the advantages of comprehensive nutrition, easy absorption, high utilization rate, and no pollution.

[0004] Patent CN 114394866 A discloses an amino acid liquid fertilizer and its preparation method and application, wherein the amino acid liquid fertilizer comprises the following raw materials in parts by weight: 140-420 parts of sugar alcohol materials, 75-240 parts of amino acid materials, 272-690 parts of element materials, 100-150 parts of glucose, 200-300 parts of water, 30-50 parts of citric acid and 50-200 parts of saponified glycerol. The invention uses amino acids, polypeptides, organic polysaccharides and polyols to hydrogen bond all trace elements required for crop growth, prevents antagonistic reactions between elements and the total carbonate, phosphate and other anions in the external environment from blocking the absorption of elements, improves the full absorption and utilization of elements by crops, and thus prevents and treats crop deficiency. However, the amino acid liquid fertilizer prepared by this method still has room for improvement in terms of crop stress resistance, soil water retention capacity and the effect of promoting crop growth rate. Summary of the invention

[0005] The purpose of the present invention is to provide a polyglutamic acid compound fertilizer capable of preventing soil compaction and a preparation method thereof, so as to solve the technical problems in the prior art that amino acid liquid fertilizers have poor stress resistance to crops, poor water retention capacity of soil and poor promotion effect on crop growth.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] The invention provides an amino acid liquid fertilizer capable of enhancing stress resistance of crops. The amino acid liquid fertilizer consists of the following components in parts by weight: 62-84 parts of xylitol, 30-43 parts of glucose, 30-42 parts of polypeptides, 8-12 parts of chelated trace elements, 10-15 parts of urea, 10-18 parts of composite fiber composite bacteria, 6-12 parts of amino acid derivatives, and 80-100 parts of deionized water, wherein the composite fiber composite bacteria are prepared from polyacrylonitrile, polyvinyl pyrrolidone, polyvinylidene fluoride and beneficial bacteria, and the amino acid derivatives are prepared from cassia twig, L-alanine and potassium hydroxide.

[0008] Preferably, the chelated trace elements are composed of 2-3 parts of EDTA-Cu, 2-3 parts of EDTA-Fe, 2-3 parts of EDTA-Mn and 2-3 parts of EDTA-Zn.

[0009] Preferably, the method for preparing the composite fiber and composite bacteria comprises the following steps:

[0010] Q1: After drying polyacrylonitrile, mix it with polyvinyl pyrrolidone and polyvinylidene fluoride, add it into N,N-dimethylformamide, stir to obtain a spinning solution, and after electrospinning, soak and dry to obtain nanofibers;

[0011] Q2: The nanofibers are immersed in a hydroxylamine hydrochloride solution, the pH is adjusted, and the solution is heated to react to obtain modified nanofibers. The modified nanofibers are added to distilled water, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and glutamic acid are added to react by heating in a water bath. After the reaction is completed, the solution is dialyzed, immersed, and dried to obtain composite nanofibers.

[0012] Q3: Add the composite nanofibers to the liquid culture medium containing beneficial bacteria, shake, take out, and dry to obtain composite fiber and composite bacteria.

[0013] In the above process, the synthesis reaction formula of the composite nanofiber is as follows:

[0014]

[0015] Preferably, in Q1, the drying temperature of polyacrylonitrile is 60-70°C, the time is 12-14h, the amount ratio of polyacrylonitrile, polyvinyl pyrrolidone, polyvinylidene fluoride and N,N-dimethylformamide is (3-6)g:(2-4)g:(0.3-0.6)g:(25-50)mL, the stirring temperature is 28-30°C, the time is 4-6h, and the electrospinning parameters are: voltage of 20KV, receiving distance of 15cm, rate of 1mL / h, indoor humidity of 25-28%, temperature of 25-30°C, soaking time in deionized water is 2-4h, drying temperature is 60-70°C, and time is 24-30h.

[0016] Preferably, in Q2, the dosage ratio of nanofibers and hydroxylamine hydrochloride solution is (2-4) g: (100-200) mL, the concentration of hydroxylamine hydrochloride solution is 0.4 mol / L, the pH is adjusted to 7 with sodium hydroxide, the heating reaction temperature is 70-80° C., the reaction time is 3-4 h, and the dosage ratio of modified nanofibers, distilled water, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and glutamic acid is (1-3) g: ( 100-300)mL: (1.19-3.57)g: (0.24-0.72)g: (1.08-3.24)g, water bath heating reaction temperature is 28-32 ℃, reaction time is 10-12h, dialyzed in deionized water for 2-2.5d, new deionized water is replaced every 6-8h during the dialysis process, soaked in a polyethylene solution with a weight fraction of 18wt% for 20-24h, dried at 40-50℃ for 10-12h.

[0017] Preferably, in Q3, the beneficial bacteria are composed of one or more combinations of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, photosynthetic bacteria and Bacillus cereus, the dosage ratio of the composite nanofibers and the liquid culture medium containing the beneficial bacteria is (10-20) g: (30-50) mL, the oscillation time is 6-8 h, the rotation speed is 160-180 rpm, and the temperature is 28-32 ° C.

[0018] Preferably, the method for preparing the amino acid derivative comprises the following steps:

[0019] S1: adding cinnamon twigs to a container containing an ethanol solution, extracting, collecting the extract, centrifuging and collecting the supernatant, adjusting the pH, slowly adding a silver nitrate solution, heating and stirring to react, cooling after the reaction is completed, and obtaining a cinnamon twig reaction solution;

[0020] S2: Mix L-alanine and potassium hydroxide and place in an ethanol solution, stir to dissolve, filter, add the cinnamon twig reaction solution, heat and stir to react, after the reaction is completed, cool and crystallize, filter, wash, and dry to obtain an amino acid derivative.

[0021] In the above process, firstly, cinnamon twig reaction liquid is obtained from cinnamon twig, and then the cinnamon twig reaction liquid is reacted with L-alanine to prepare the amino acid derivative through a condensation reaction.

[0022] Preferably, in S1, the dosage ratio of cinnamon twig, ethanol solution and silver nitrate solution is (4-8) g: (60-120) mL: (1.5-3) mL, the volume fraction of ethanol solution is 75 vt%, extraction is carried out by microwave-ultrasound assisted extraction technology, extraction is carried out at 600 W for 20-25 min, the centrifugation time is 10-12 min, the centrifugation speed is 8000-10000 rpm, pH=9 is adjusted with ammonia water, the volume fraction of silver nitrate solution is 1 vt%, the heating temperature is 140-150° C., and the reaction time is 3-6 h; in S2, the dosage ratio of L-alanine, potassium hydroxide, ethanol solution and cinnamon twig reaction solution is (8-10) g: (8-10) mL: (45-50) mL: (10-15) mL, the heating and stirring temperature is 40-60° C., the stirring time is 3-5 h, and the mixture is washed with ethyl acetate 3-5 times.

[0023] Preferably, the method for preparing the amino acid liquid fertilizer capable of enhancing the stress resistance of crops comprises the following steps:

[0024] Step (1): mixing xylitol, polypeptide, chelated trace elements, urea and amino acid derivatives to obtain a complex;

[0025] Step (2): mixing the composite with glucose, composite fiber, composite bacteria and deionized water to obtain an amino acid liquid fertilizer that can enhance the stress resistance of crops.

[0026] Preferably, in step (1), the mixing time is 4-6 hours; and in step (2), the mixing and stirring time is 2-3 hours.

[0027] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0028] 1. The present invention first uses polyacrylonitrile, polyvinyl pyrrolidone, polyvinylidene fluoride, hydroxylamine hydrochloride, glutamic acid and beneficial bacteria as raw materials to prepare composite fiber composite bacteria, and then uses cassia twig and L-alanine as raw materials to prepare amino acid derivatives, and adds the two into amino acid liquid fertilizer, which can effectively improve the stress resistance of crops, the water retention of soil and promote the growth of crops.

[0029] 2. The present invention uses polyacrylonitrile, polyvinyl pyrrolidone, hydroxylamine hydrochloride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and glutamic acid as raw materials to prepare composite nanofibers, which are used as carriers to fix beneficial bacteria to obtain composite fiber composite bacteria, which are added to amino acid liquid fertilizer to effectively improve fertilizer utilization, improve soil water retention capacity, promote crop growth and enhance crop stress resistance. The beneficial bacteria in the composite fiber composite bacteria can decompose organic matter and release nutrients, while promoting the development of plant roots and enhancing the root system's ability to absorb nutrients. The presence of the composite fiber composite bacteria can make the amino acid liquid fertilizer release nutrients evenly and lastingly, thereby improving the utilization rate of the liquid fertilizer; the beneficial bacteria in the composite fiber composite bacteria can secrete substances such as growth hormones, further stimulate plant growth, make crop growth more robust, enable crops to maintain a good growth state under adverse conditions, and increase stress resistance; the composite fiber composite bacteria are added to the soil to effectively improve the physical and chemical properties of the soil, thereby being beneficial to the growth of the root system and the absorption of nutrients, and creating a good growth environment for crops.

[0030] 3. The present invention first obtains cinnamon twig reaction liquid from cinnamon twig, and then reacts it with L-alanine to prepare amino acid derivatives, which are added to amino acid liquid fertilizer to improve the biological activity and stability of the liquid fertilizer, thereby promoting the growth of crops and enhancing the stress resistance of crops. L-alanine can absorb or release hydrogen ions to maintain the liquid fertilizer within a suitable pH range, which is beneficial to the storage and use of the fertilizer. L-amino acids can be combined with other nutrient elements to form stable complexes or chelates, thereby facilitating the absorption of nutrients by plant roots. Amino acid derivatives can be used as plant growth regulators to promote plant cell division and elongation, increase leaf area and chlorophyll content, and improve photosynthesis efficiency. Amino acid derivatives can also improve the activity of antioxidant enzymes in plants, remove active oxygen, reduce the damage of adversity to plants, and enhance stress resistance. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1

[0033] This embodiment discloses a method for preparing a composite fiber and composite bacteria, comprising the following steps:

[0034] Q1: 4.5g polyacrylonitrile was dried at 70℃ for 12h, mixed with 3g polyvinyl pyrrolidone and 0.45g polyvinylidene fluoride, added to 39mL N,N-dimethylformamide, stirred at 30℃ for 6h to obtain a spinning solution, and electrospinned. The electrospinning parameters were: voltage of 20KV, receiving distance of 15cm, rate of 1mL / h, indoor humidity of 28%, temperature of 28℃. After electrospinning, the solution was soaked in deionized water for 4h and dried at 70℃ for 24h to obtain nanofibers.

[0035] Q2: 3g of nanofibers were immersed in 150mL of 0.4mol / L hydroxylamine hydrochloride solution, and the pH was adjusted to 7 with sodium hydroxide, and the reaction was heated at 80°C for 4h to obtain modified nanofibers. 2g of modified nanofibers were added to 200mL of distilled water, and then 2.38g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.48g of N-hydroxysuccinimide and 2.16g of glutamic acid were added, and the reaction was heated in a water bath at 32°C for 12h. After the reaction was completed, the nanofibers were dialyzed in deionized water for 2d, and new deionized water was replaced every 6h during the dialysis process. The nanofibers were immersed in a polyethylene solution with a weight fraction of 18wt% for 24h, and dried at 50°C for 12h to obtain composite nanofibers.

[0036] Q3: Add 15 g of composite nanofibers to 40 mL of liquid culture medium containing Bacillus subtilis, shake at 30°C and 180 rpm for 8 h, take out, and dry to obtain composite fiber and composite bacteria.

[0037] This embodiment discloses a method for preparing an amino acid derivative, comprising the following steps:

[0038] S1: 6 g of cinnamon twigs were added to a container containing 90 mL of 75 vt% ethanol solution, and extracted by microwave-ultrasound-assisted extraction technology at 600 W for 25 min, and the extract was collected. After centrifugation at 10,000 rpm for 12 min, the supernatant was collected, and the pH was adjusted to 9 with ammonia water, and 2.75 mL of 1 vt% silver nitrate solution was slowly added, and the mixture was heated and stirred at 140 ° C for 4 h. After the reaction was completed, it was cooled to obtain a cinnamon twig reaction solution;

[0039] S2: Mix 9 g of L-alanine and 9 mL of potassium hydroxide and place in 47.5 mL of ethanol solution, stir to dissolve, filter, add 12.5 mL of cinnamon twig reaction solution, heat and stir at 60°C for 5 h, after the reaction is completed, cool and crystallize, filter, wash 3 times with ethyl acetate, and dry to obtain an amino acid derivative.

[0040] The present embodiment discloses an amino acid liquid fertilizer capable of enhancing crop stress resistance, which is composed of the following ingredients in parts by weight: 73 parts of xylitol, 36 parts of glucose, 36 parts of polypeptide, 2.5 parts of EDTA-Cu, 2.5 parts of EDTA-Fe, 2.5 parts of EDTA-Mn, 2.5 parts of EDTA-Zn, 12.5 parts of urea, 14 parts of composite fiber composite bacteria, 9 parts of amino acid derivatives, and 90 parts of deionized water.

[0041] This embodiment discloses a method for preparing an amino acid liquid fertilizer that can enhance crop stress resistance, comprising the following steps:

[0042] Step (1): mixing xylitol, polypeptide, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Zn, urea and amino acid derivatives for 6 hours to obtain a complex;

[0043] Step (2): the complex is mixed with glucose, composite fiber, composite bacteria and deionized water and stirred for 3 hours to obtain an amino acid liquid fertilizer that can enhance crop stress resistance.

[0044] Example 2

[0045] This embodiment discloses a method for preparing a composite fiber and composite bacteria, comprising the following steps:

[0046] Q1: 3g of polyacrylonitrile was dried at 70℃ for 12h, mixed with 2g of polyvinyl pyrrolidone and 0.3g of polyvinylidene fluoride, added to 25mL of N,N-dimethylformamide, stirred at 30℃ for 6h to obtain a spinning solution, and subjected to electrospinning treatment. The electrospinning parameters were: voltage of 20KV, receiving distance of 15cm, rate of 1mL / h, indoor humidity of 28%, temperature of 28℃. After electrospinning treatment, the solution was soaked in deionized water for 4h and dried at 70℃ for 24h to obtain nanofibers.

[0047] Q2: 2g of nanofibers were immersed in 100mL of 0.4mol / L hydroxylamine hydrochloride solution, and the pH was adjusted to 7 with sodium hydroxide, and the reaction was heated at 80°C for 4h to obtain modified nanofibers. 1g of modified nanofibers was added to 100mL of distilled water, and then 1.19g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.24g of N-hydroxysuccinimide and 1.08g of glutamic acid were added, and the reaction was heated in a water bath at 32°C for 12h. After the reaction was completed, the nanofibers were dialyzed in deionized water for 2d, and new deionized water was replaced every 6h during the dialysis process. The nanofibers were immersed in a polyethylene solution with a weight fraction of 18wt% for 24h, and dried at 50°C for 12h to obtain composite nanofibers.

[0048] Q3: Add 10 g of composite nanofibers to 30 mL of liquid culture medium containing Bacillus subtilis, shake at 30°C and 180 rpm for 8 h, take out, and dry to obtain composite fiber and composite bacteria.

[0049] This embodiment discloses a method for preparing an amino acid derivative, comprising the following steps:

[0050] S1: 4 g of cinnamon twigs were added to a container containing 60 mL of 75 vt% ethanol solution, and extracted by microwave-ultrasound-assisted extraction technology at 600 W for 25 min, and the extract was collected. After centrifugation at 10,000 rpm for 12 min, the supernatant was collected, and the pH was adjusted to 9 with ammonia water, and 1.5 mL of 1 vt% silver nitrate solution was slowly added, and the mixture was heated and stirred at 140 ° C for 4 h. After the reaction was completed, it was cooled to obtain a cinnamon twig reaction solution;

[0051] S2: Mix 8 g of L-alanine and 10 mL of potassium hydroxide and place in 45 mL of ethanol solution, stir to dissolve, filter, add 10 mL of cinnamon twig reaction solution, heat and stir at 60°C for 5 h, after the reaction is completed, cool and crystallize, filter, wash 3 times with ethyl acetate, and dry to obtain an amino acid derivative.

[0052] The present embodiment discloses an amino acid liquid fertilizer capable of enhancing crop stress resistance, which is composed of the following ingredients in parts by weight: 62 parts of xylitol, 30 parts of glucose, 30 parts of polypeptide, 2 parts of EDTA-Cu, 2 parts of EDTA-Fe, 2 parts of EDTA-Mn, 2 parts of EDTA-Zn, 10 parts of urea, 10 parts of composite fiber composite bacteria, 6 parts of amino acid derivatives, and 80 parts of deionized water.

[0053] This embodiment discloses a method for preparing an amino acid liquid fertilizer that can enhance crop stress resistance, comprising the following steps:

[0054] Step (1): mixing xylitol, polypeptide, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Zn, urea and amino acid derivatives for 6 hours to obtain a complex;

[0055] Step (2): the complex is mixed with glucose, composite fiber, composite bacteria and deionized water and stirred for 3 hours to obtain an amino acid liquid fertilizer that can enhance crop stress resistance.

[0056] Example 3

[0057] This embodiment discloses a method for preparing a composite fiber and composite bacteria, comprising the following steps:

[0058] Q1: 6g of polyacrylonitrile was dried at 70℃ for 12h, mixed with 4g of polyvinyl pyrrolidone and 0.6g of polyvinylidene fluoride, added to 50mL of N,N-dimethylformamide, stirred at 30℃ for 6h to obtain a spinning solution, and subjected to electrospinning treatment. The electrospinning parameters were: voltage of 20KV, receiving distance of 15cm, rate of 1mL / h, indoor humidity of 28%, temperature of 28℃. After electrospinning treatment, the solution was soaked in deionized water for 4h and dried at 70℃ for 24h to obtain nanofibers.

[0059] Q2: 4g of nanofibers were immersed in 200mL of 0.4mol / L hydroxylamine hydrochloride solution, adjusted to pH=7 with sodium hydroxide, and heated at 80°C for 4h to obtain modified nanofibers. 3g of modified nanofibers were added to 300mL of distilled water, and then 3.57g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.72g of N-hydroxysuccinimide and 3.24g of glutamic acid were added, and heated in a water bath at 32°C for 12h. After the reaction was completed, the nanofibers were dialyzed in deionized water for 2d, and new deionized water was replaced every 6h during the dialysis process. The nanofibers were immersed in a polyethylene solution with a weight fraction of 18wt% for 24h, and dried at 50°C for 12h to obtain composite nanofibers.

[0060] Q3: Add 20 g of composite nanofibers to 50 mL of liquid culture medium containing Bacillus subtilis, shake at 30°C and 180 rpm for 8 h, take out, and dry to obtain composite fiber and composite bacteria.

[0061] This embodiment discloses a method for preparing an amino acid derivative, comprising the following steps:

[0062] S1: 8 g of cinnamon twigs were added to a container containing 120 mL of 75 vt% ethanol solution, and extracted by microwave-ultrasound-assisted extraction technology at 600 W for 25 min, and the extract was collected. After centrifugation at 10,000 rpm for 12 min, the supernatant was collected, and the pH was adjusted to 9 with ammonia water, and 3 mL of 1 vt% silver nitrate solution was slowly added, and the mixture was heated and stirred at 140 ° C for 4 h. After the reaction was completed, it was cooled to obtain a cinnamon twig reaction solution;

[0063] S2: Mix 10g L-alanine and 8mL potassium hydroxide and place in 50mL ethanol solution, stir to dissolve, filter, add 15mL cinnamon twig reaction solution, heat and stir at 60℃ for 5h, after the reaction is completed, cool and crystallize, filter, wash 3 times with ethyl acetate, and dry to obtain amino acid derivatives.

[0064] The present embodiment discloses an amino acid liquid fertilizer capable of enhancing crop stress resistance, which is composed of the following ingredients in parts by weight: 84 parts of xylitol, 43 parts of glucose, 42 parts of polypeptide, 2.1 parts of EDTA-Cu, 2.2 parts of EDTA-Fe, 2.5 parts of EDTA-Mn, 2.7 parts of EDTA-Zn, 15 parts of urea, 18 parts of composite fiber composite bacteria, 12 parts of amino acid derivatives, and 100 parts of deionized water.

[0065] This embodiment discloses a method for preparing an amino acid liquid fertilizer that can enhance crop stress resistance, comprising the following steps:

[0066] Step (1): mixing xylitol, polypeptide, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Zn, urea and amino acid derivatives for 6 hours to obtain a complex;

[0067] Step (2): the complex is mixed with glucose, composite fiber, composite bacteria and deionized water and stirred for 3 hours to obtain an amino acid liquid fertilizer that can enhance crop stress resistance.

[0068] Example 4

[0069] This embodiment discloses a method for preparing a composite fiber and composite bacteria, comprising the following steps:

[0070] Q1: 4g of polyacrylonitrile was dried at 70℃ for 12h, mixed with 3.5g of polyvinyl pyrrolidone and 0.4g of polyvinylidene fluoride, added to 30mL of N,N-dimethylformamide, stirred at 30℃ for 6h to obtain a spinning solution, and subjected to electrospinning treatment. The electrospinning parameters were: voltage of 20KV, receiving distance of 15cm, rate of 1mL / h, indoor humidity of 28%, temperature of 28℃. After electrospinning treatment, the solution was soaked in deionized water for 4h and dried at 70℃ for 24h to obtain nanofibers.

[0071] Q2: 2.5 g of nanofibers were immersed in 120 mL of 0.4 mol / L hydroxylamine hydrochloride solution, and the pH was adjusted to 7 with sodium hydroxide, and the reaction was heated at 80°C for 4 h to obtain modified nanofibers. 2.5 g of modified nanofibers were added to 150 mL of distilled water, and then 1.85 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.36 g of N-hydroxysuccinimide and 1.58 g of glutamic acid were added, and the reaction was heated in a water bath at 32°C for 12 h. After the reaction was completed, the nanofibers were placed in deionized water for 2 days, and new deionized water was replaced every 6 hours during the dialysis process. The nanofibers were immersed in a polyethylene solution with a weight fraction of 18 wt% for 24 h, and dried at 50°C for 12 h to obtain composite nanofibers.

[0072] Q3: Add 12 g of composite nanofibers to 35 mL of liquid culture medium containing Bacillus subtilis, shake at 30°C and 180 rpm for 8 h, take out, and dry to obtain composite fiber and composite bacteria.

[0073] This embodiment discloses a method for preparing an amino acid derivative, comprising the following steps:

[0074] S1: 5 g of cinnamon twigs were added to a container containing 80 mL of 75 vt% ethanol solution, and extracted by microwave-ultrasound-assisted extraction technology at 600 W for 25 min, and the extract was collected. After centrifugation at 10,000 rpm for 12 min, the supernatant was collected, and the pH was adjusted to 9 with ammonia water, and 2 mL of 1 vt% silver nitrate solution was slowly added, and the mixture was heated and stirred at 140 ° C for 4 h. After the reaction was completed, it was cooled to obtain a cinnamon twig reaction solution;

[0075] S2: Mix 8.5 g of L-alanine and 8.5 mL of potassium hydroxide and place in 46 mL of ethanol solution, stir to dissolve, filter, add 12 mL of cinnamon twig reaction solution, heat and stir at 60°C for 5 h, after the reaction is completed, cool and crystallize, filter, wash 3 times with ethyl acetate, and dry to obtain an amino acid derivative.

[0076] The present embodiment discloses an amino acid liquid fertilizer capable of enhancing crop stress resistance, which is composed of the following ingredients in parts by weight: 68 parts of xylitol, 33 parts of glucose, 32 parts of polypeptide, 2 parts of EDTA-Cu, 2.1 parts of EDTA-Fe, 2.4 parts of EDTA-Mn, 2 parts of EDTA-Zn, 11 parts of urea, 12 parts of composite fiber composite bacteria, 8 parts of amino acid derivatives, and 85 parts of deionized water.

[0077] This embodiment discloses a method for preparing an amino acid liquid fertilizer that can enhance crop stress resistance, comprising the following steps:

[0078] Step (1): mixing xylitol, polypeptide, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Zn, urea and amino acid derivatives for 6 hours to obtain a complex;

[0079] Step (2): the complex is mixed with glucose, composite fiber, composite bacteria and deionized water and stirred for 3 hours to obtain an amino acid liquid fertilizer that can enhance crop stress resistance.

[0080] Example 5

[0081] This embodiment discloses a method for preparing a composite fiber and composite bacteria, comprising the following steps:

[0082] Q1: 5g of polyacrylonitrile was dried at 70℃ for 12h, mixed with 2.5g of polyvinyl pyrrolidone and 0.5g of polyvinylidene fluoride, added to 45mL of N,N-dimethylformamide, stirred at 30℃ for 6h to obtain a spinning solution, and then electrospun. The electrospinning parameters were: voltage of 20KV, receiving distance of 15cm, rate of 1mL / h, indoor humidity of 28%, temperature of 28℃. After electrospinning, the solution was soaked in deionized water for 4h and dried at 70℃ for 24h to obtain nanofibers.

[0083] Q2: 3.5g of nanofibers were immersed in 180mL of 0.4mol / L hydroxylamine hydrochloride solution, and the pH was adjusted to 7 with sodium hydroxide, and the reaction was heated at 80℃ for 4h to obtain modified nanofibers. 1.5g of modified nanofibers were added to 250mL of distilled water, and then 2.97g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.6g of N-hydroxysuccinimide and 2.77g of glutamic acid were added, and the reaction was heated in a water bath at 32℃ for 12h. After the reaction was completed, the nanofibers were placed in deionized water for 2d, and the deionized water was replaced every 6h during the dialysis process. The nanofibers were immersed in a polyethylene solution with a weight fraction of 18wt% for 24h, and dried at 50℃ for 12h to obtain composite nanofibers.

[0084] Q3: Add 18 g of composite nanofibers to 45 mL of liquid culture medium containing Bacillus subtilis, shake at 30°C and 180 rpm for 8 h, take out, and dry to obtain composite fiber and composite bacteria.

[0085] This embodiment discloses a method for preparing an amino acid derivative, comprising the following steps:

[0086] S1: 7 g of cinnamon twigs were added to a container containing 100 mL of 75 vt% ethanol solution, and extracted by microwave-ultrasound-assisted extraction technology at 600 W for 25 min, and the extract was collected. After centrifugation at 10,000 rpm for 12 min, the supernatant was collected, and the pH was adjusted to 9 with ammonia water, and 2.5 mL of 1 vt% silver nitrate solution was slowly added, and the mixture was heated and stirred at 140 ° C for 4 h. After the reaction was completed, it was cooled to obtain a cinnamon twig reaction solution;

[0087] S2: Mix 9.5 g of L-alanine and 9.5 mL of potassium hydroxide and place in 48 mL of ethanol solution, stir to dissolve, filter, add 14 mL of cinnamon twig reaction solution, heat and stir at 60°C for 5 h, after the reaction is completed, cool and crystallize, filter, wash 3 times with ethyl acetate, and dry to obtain an amino acid derivative.

[0088] The present embodiment discloses an amino acid liquid fertilizer capable of enhancing crop stress resistance, which is composed of the following ingredients in parts by weight: 79 parts of xylitol, 40 parts of glucose, 38 parts of polypeptide, 2.8 parts of EDTA-Cu, 2.7 parts of EDTA-Fe, 2 parts of EDTA-Mn, 2.3 parts of EDTA-Zn, 14 parts of urea, 16 parts of composite fiber and composite bacteria, 10 parts of amino acid derivatives, and 95 parts of deionized water.

[0089] This embodiment discloses a method for preparing an amino acid liquid fertilizer that can enhance crop stress resistance, comprising the following steps:

[0090] Step (1): mixing xylitol, polypeptide, EDTA-Cu, EDTA-Fe, EDTA-Mn, EDTA-Zn, urea and amino acid derivatives for 6 hours to obtain a complex;

[0091] Step (2): the complex is mixed with glucose, composite fiber, composite bacteria and deionized water and stirred for 3 hours to obtain an amino acid liquid fertilizer that can enhance crop stress resistance.

[0092] Comparative Example 1

[0093] Comparative Example 1 Compared with Example 1, in the preparation process of the composite fiber and composite bacteria in Comparative Example 1, no polyacrylonitrile is added, and other conditions remain unchanged.

[0094] Comparative Example 2

[0095] Comparative Example 2 Compared with Example 1, in the preparation process of the composite fiber and composite bacteria in Comparative Example 2, Bacillus subtilis was not added, and other conditions remained unchanged.

[0096] Comparative Example 3

[0097] Comparative Example 3 Compared with Example 1, in the preparation process of the amino acid derivatives in Comparative Example 3, cinnamon twigs were not added, and other conditions remained unchanged.

[0098] Comparative Example 4

[0099] Comparative Example 4 Compared with Example 1, in the preparation process of the amino acid derivatives in Comparative Example 4, L-alanine was not added, and other conditions remained unchanged.

[0100] Comparative Example 5

[0101] Comparative Example 5 Compared with Example 1, in the preparation process of the amino acid liquid fertilizer in Comparative Example 5, no composite fiber and composite bacteria were added, and other conditions remained unchanged.

[0102] Comparative Example 6

[0103] Comparative Example 6 Compared with Example 1, in the preparation process of the amino acid liquid fertilizer in Comparative Example 6, no amino acid derivatives were added, and other conditions remained unchanged.

[0104] After the cotton seeds were delinted with concentrated sulfuric acid and disinfected with 10vt% hydrogen peroxide, the seeds were planted in an area of ​​16cm×25cm×10cm, and 30 seeds were planted. The soil in each group of areas was irrigated with an equal amount of 0.3vt% sodium chloride solution every two days to simulate a salt stress environment. After planting, a layer of soil was covered and watered until the soil was moistened. The daylight and night time were 12h respectively, the light temperature was 30°C, and the dark temperature was 25°C. Watering was performed every day to make the soil moisture content higher than 58%. The liquid fertilizer prepared by Examples 1-5 and Comparative Examples 1-6 was applied once every 3 days, the germination of the seeds was observed, and the germination rate after 10 days of planting was calculated. After the seeds germinated for 15 days, the peroxidase activity was determined by the guaiacol colorimetric method, the proline content was determined by the acid ninhydrin colorimetric method, and the chlorophyll content was determined by the ethanol acetone extraction method. At maturity, the biomass of the cotton was measured, and the test results are shown in Table 1:

[0105] Table 1

[0106]

[0107] It can be seen from the test results in Table 1 that the amino acid liquid fertilizer prepared in Examples 1-5 of the present invention has the effect of improving crop stress resistance and increasing crop biomass. By comparing Comparative Example 1 with Examples 1-5, it can be seen that adding polyacrylonitrile can make the amino acid liquid fertilizer have the effect of improving crop stress resistance and increasing crop biomass; by comparing Comparative Example 2 with Examples 1-5, it can be seen that adding Bacillus subtilis can make the amino acid liquid fertilizer have the effect of improving crop stress resistance and increasing crop biomass; by comparing Comparative Example 3 with Examples 1-5, it can be seen that adding cassia twig can make the amino acid liquid fertilizer have the effect of improving crop stress resistance and increasing crop biomass; by comparing Comparative Example 4 with Examples 1-5, it can be seen that adding L-alanine can make the amino acid liquid fertilizer have the effect of improving crop stress resistance and increasing crop biomass; by comparing Comparative Example 5 with Examples 1-5, it can be seen that adding composite fiber composite bacteria can make the amino acid liquid fertilizer have the effect of improving crop stress resistance and increasing crop biomass; by comparing Comparative Example 6 with Examples 1-5, it can be seen that adding amino acid derivatives can make the amino acid liquid fertilizer have the effect of improving crop stress resistance and increasing crop biomass.

[0108] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0109] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. Amino acid liquid fertilizer that can enhance crop stress resistance, characterized in that: The invention is composed of the following ingredients in parts by weight: 62-84 parts of xylitol, 30-43 parts of glucose, 30-42 parts of polypeptides, 8-12 parts of chelated trace elements, 10-15 parts of urea, 10-18 parts of composite fiber and composite bacteria, 6-12 parts of amino acid derivatives, and 80-100 parts of deionized water, wherein the composite fiber and composite bacteria are prepared from polyacrylonitrile, polyvinyl pyrrolidone, polyvinylidene fluoride and beneficial bacteria, and the amino acid derivatives are prepared from cassia twig, L-alanine and potassium hydroxide.

2. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 1, characterized in that: The chelated trace elements are composed of 2-3 parts of EDTA-Cu, 2-3 parts of EDTA-Fe, 2-3 parts of EDTA-Mn and 2-3 parts of EDTA-Zn.

3. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 1, characterized in that: The preparation method of the composite fiber and composite bacteria comprises the following steps: Q1: After drying polyacrylonitrile, mix it with polyvinyl pyrrolidone and polyvinylidene fluoride, add it into N,N-dimethylformamide, stir to obtain a spinning solution, and after electrospinning, soak and dry to obtain nanofibers; Q2: The nanofibers are immersed in a hydroxylamine hydrochloride solution, the pH is adjusted, and the solution is heated to react to obtain modified nanofibers. The modified nanofibers are added to distilled water, and then 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and glutamic acid are added to react by heating in a water bath. After the reaction is completed, the solution is dialyzed, immersed, and dried to obtain composite nanofibers. Q3: Add the composite nanofibers to the liquid culture medium containing beneficial bacteria, shake, take out, and dry to obtain composite fiber and composite bacteria.

4. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 3, characterized in that: In the Q1, the drying temperature of polyacrylonitrile is 60-70°C, the time is 12-14h, the amount ratio of polyacrylonitrile, polyvinyl pyrrolidone, polyvinylidene fluoride and N,N-dimethylformamide is (3-6)g: (2-4)g: (0.3-0.6)g: (25-50)mL, the stirring temperature is 28-30°C, the time is 4-6h, and the electrospinning parameters are: voltage is 20KV, receiving distance is 15cm, rate is 1mL / h, indoor humidity is 25-28%, temperature is 25-30°C, soaking time in deionized water is 2-4h, drying temperature is 60-70°C, and time is 24-30h.

5. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 3, characterized in that: In the Q2, the dosage ratio of the nanofibers and the hydroxylamine hydrochloride solution is (2-4) g: (100-200) mL, the concentration of the hydroxylamine hydrochloride solution is 0.4 mol / L, the pH is adjusted to 7 with sodium hydroxide, the heating reaction temperature is 70-80° C., the reaction time is 3-4 h, and the dosage ratio of the modified nanofibers, distilled water, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and glutamic acid is (1-3) g: (10 0-300)mL: (1.19-3.57)g: (0.24-0.72)g: (1.08-3.24)g, water bath heating reaction temperature is 28-32 ℃, reaction time is 10-12h, dialyzed in deionized water for 2-2.5d, new deionized water is replaced every 6-8h during the dialysis process, immersed in a polyethylene solution with a weight fraction of 18wt% for 20-24h, and dried at 40-50℃ for 10-12h.

6. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 3, characterized in that: In the Q3, the beneficial bacteria are composed of one or more combinations of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, photosynthetic bacteria and Bacillus cereus, the dosage ratio of the composite nanofibers and the liquid culture medium containing the beneficial bacteria is (10-20) g: (30-50) mL, the oscillation time is 6-8 h, the rotation speed is 160-180 rpm, and the temperature is 28-32°C.

7. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 1, characterized in that: The preparation method of the amino acid derivative comprises the following steps: S1: adding cinnamon twigs to a container containing an ethanol solution, extracting, collecting the extract, centrifuging and collecting the supernatant, adjusting the pH, slowly adding a silver nitrate solution, heating and stirring to react, cooling after the reaction is completed, and obtaining a cinnamon twig reaction solution; S2: Mix L-alanine and potassium hydroxide and place in an ethanol solution, stir to dissolve, filter, add the cinnamon twig reaction solution, heat and stir to react, after the reaction is completed, cool and crystallize, filter, wash, and dry to obtain an amino acid derivative.

8. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 7, characterized in that: In the S1, the dosage ratio of cinnamon twig, ethanol solution and silver nitrate solution is (4-8) g: (60-120) mL: (1.5-3) mL, the volume fraction of ethanol solution is 75 vt%, extraction is carried out by microwave-ultrasound assisted extraction technology, extraction is carried out at 600 W for 20-25 min, the centrifugation time is 10-12 min, the centrifugation speed is 8000-10000 rpm, pH=9 is adjusted with ammonia water, the volume fraction of silver nitrate solution is 1 vt%, the heating temperature is 140-150° C., and the reaction time is 3-6 h. In the S2, the dosage ratio of L-alanine, potassium hydroxide, ethanol solution and cinnamon twig reaction solution is (8-10) g: (8-10) mL: (45-50) mL: (10-15) mL, the heating and stirring temperature is 40-60° C., the stirring time is 3-5 h, and the mixture is washed with ethyl acetate 3-5 times.

9. A method for preparing an amino acid liquid fertilizer capable of enhancing crop stress resistance as claimed in any one of claims 1 to 8, characterized in that: The following steps are involved: Step (1): mixing xylitol, polypeptide, chelated trace elements, urea and amino acid derivatives to obtain a complex; Step (2): mixing the composite with glucose, composite fiber, composite bacteria and deionized water to obtain an amino acid liquid fertilizer that can enhance the stress resistance of crops.

10. The amino acid liquid fertilizer capable of enhancing crop stress resistance according to claim 9, characterized in that: In the step (1), the mixing time is 4-6 hours; in the step (2), the mixing and stirring time is 2-3 hours.

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