Hypochlorous acid composite disinfectant and preparation method thereof

Abstract

The present invention relates to a hypochlorous acid composite disinfectant and a preparation method thereof, and relates to the technical field of disinfectants. The hypochlorous acid composite disinfectant comprises the following components by weight: 60-75 parts of hypochlorous acid aqueous solution, 3-8 parts of scutellaria baicalensis extract, 19.5-25 parts of polyethylene glycol, 2-5 parts of surfactant and 0.5-2 parts of phosphate. The present invention mainly uses hypochlorous acid and introduces an appropriate proportion of scutellaria baicalensis extract as a disinfection component, which significantly enhances the overall disinfection effect of the obtained disinfectant; at the same time, other components such as polyethylene glycol and phosphate are matched, which significantly enhances the stability of the obtained disinfectant, and has efficient bactericidal performance against pathogenic microorganisms such as African swine fever virus, which can meet the sterilization and disinfection needs of farms.

Description

Technical Field

[0001] The present invention relates to the technical field of disinfectants, and in particular to a hypochlorous acid composite disinfectant and a preparation method thereof. Background Art

[0002] In the current disinfectant market, with the significant improvement of global awareness of public health and safety, especially in the key field of animal husbandry, the demand for efficient and safe disinfectants is becoming increasingly urgent. African swine fever, as a highly contagious and highly lethal viral disease, has brought huge economic losses and prevention and control challenges to the global animal husbandry industry. Traditional disinfectants often show limited disinfection effects, high irritation, and poor stability when dealing with stubborn viruses such as African swine fever, making it difficult to meet the urgent needs of farms for efficient and long-lasting disinfection.

[0003] Hypochlorous acid (HClO) has attracted much attention in the field of disinfectants due to its broad-spectrum and highly effective disinfection properties. It can effectively destroy the cell structure of microorganisms and has a strong killing ability against a variety of pathogenic microorganisms, including African swine fever virus. However, pure hypochlorous acid solution is easy to decompose and has poor stability in actual applications, which greatly limits its application effect in complex environments such as farms.

[0004] In order to improve the stability and disinfection efficiency of hypochlorous acid, the industry has begun to explore the possibility of using it in combination with other ingredients. In particular, natural plant extracts have gradually become a new hotspot in the research and development of disinfectants because they are safe, environmentally friendly and rich in a variety of biologically active ingredients. At present, there are no related technical reports on the combined use of hypochlorous acid and scutellaria baicalensis to prepare disinfectants in the prior art. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a hypochlorous acid composite disinfectant and a preparation method thereof.

[0006] In a first aspect, the present invention provides a hypochlorous acid composite disinfectant, which comprises the following components in parts by weight:

[0007] 60-75 parts of hypochlorous acid aqueous solution, 3-8 parts of scutellaria baicalensis extract, 19.5-25 parts of polyethylene glycol, 2-5 parts of surfactant and 0.5-2 parts of phosphate.

[0008] Furthermore, the hypochlorous acid composite disinfectant comprises the following components in parts by weight:

[0009] 71 parts of hypochlorous acid aqueous solution, 5.5 parts of scutellaria baicalensis extract, 20 parts of polyethylene glycol, 2.5 parts of surfactant and 1 part of phosphate.

[0010] Furthermore, the preparation method of the extract of the Herba Lycopodii var.

[0011] The scutellaria baicalensis is crushed, and then added into water for reflux extraction to obtain an extract;

[0012] The extract is concentrated and dried to obtain the Herba Lycopodii var.

[0013] Furthermore, the working condition parameters of the reflux extraction include: temperature of 100-110° C., time of 1-3 hours, and number of times of 1-3 times.

[0014] Furthermore, the weight ratio of the water to the Myrica ovata is (5-10):1.

[0015] Furthermore, the concentration of the hypochlorous acid aqueous solution is 50 mg / L to 100 mg / L.

[0016] Furthermore, the surfactant includes monoethanolamine lauryl sulfate; and the phosphate includes at least one of sodium phosphate and disodium hydrogen phosphate.

[0017] Furthermore, the polyethylene glycol includes at least one of polyethylene glycol 400 and polyethylene glycol 600.

[0018] In a second aspect, the present invention provides a method for preparing the hypochlorous acid composite disinfectant according to any one of the first aspects, the method for preparing the hypochlorous acid composite disinfectant comprising the following steps:

[0019] The hypochlorous acid aqueous solution, polyethylene glycol, a surfactant and a phosphate are stirred and mixed to obtain a mixture;

[0020] The mixed material and the extract of the Herba Lycopodii are mixed by ultrasonic stirring to obtain the hypochlorous acid composite disinfectant.

[0021] Furthermore, the working condition parameters of the ultrasonic stirring and mixing include: ultrasonic power of 300-500W, stirring speed of 100-200rpm, and stirring time of 5-15 minutes.

[0022] The above technical solution provided by the present invention has at least the following advantages compared with the prior art:

[0023] The invention provides a hypochlorous acid composite disinfectant and a preparation method thereof. The invention mainly uses hypochlorous acid and introduces an appropriate proportion of an extract of a Herba Lycopodii as a disinfectant component, thereby significantly enhancing the overall disinfection effect of the obtained disinfectant. At the same time, other components such as polyethylene glycol and phosphate are combined to significantly enhance the stability of the obtained disinfectant, and the disinfectant has efficient bactericidal performance against pathogenic microorganisms such as African swine fever virus, thereby meeting the sterilization and disinfection needs of farms.

[0024] 1) Hypochlorous acid (HClO), as a strong oxidant, can destroy the cell wall and cell membrane of microorganisms, penetrate the interior of cells, react with key biological molecules such as proteins and DNA in cells, and cause the microorganisms to lose activity, thereby achieving the purpose of sterilization and disinfection. Hypochlorous acid has a good killing effect on a variety of microorganisms such as bacteria, viruses, and fungi, and is one of the effective ingredients in disinfectants, but hypochlorous acid is easy to decompose, resulting in poor product stability. Therefore, the present invention introduces the extract of the scutellaria baicalensis, which is rich in a variety of biologically active ingredients, such as flavonoids, polyphenols, etc. These ingredients have multiple biological activities such as antibacterial, anti-inflammatory, and antioxidant. In the composite disinfectant, the extract of the scutellaria baicalensis can form a synergistic effect with hypochlorous acid, which can enhance the disinfection effect of hypochlorous acid on the one hand, and on the other hand, it may further expand the disinfection spectrum and improve the disinfection efficiency through mechanisms such as interfering with microbial metabolism and destroying cell structure.

[0025] 2) Polyethylene glycol (PEG) has good water solubility and stability. Together with other components such as phosphate, it mainly plays the role of auxiliary stability and pH buffering in compound disinfectants, further improving the stability and service life of the product. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described in combination with the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0028] In a first aspect, the present invention provides a hypochlorous acid composite disinfectant, which comprises the following components in parts by weight:

[0029] 60-75 parts of hypochlorous acid aqueous solution, 3-8 parts of scutellaria baicalensis extract, 19.5-25 parts of polyethylene glycol, 2-5 parts of surfactant and 0.5-2 parts of phosphate.

[0030] The invention provides a hypochlorous acid composite disinfectant, which mainly uses hypochlorous acid and introduces an appropriate proportion of an extract of the Herba Lycopodii as a disinfectant component, thereby significantly enhancing the overall disinfection effect of the obtained disinfectant; at the same time, other components such as polyethylene glycol and phosphate are combined to significantly enhance the stability of the obtained disinfectant, and the disinfectant has efficient bactericidal performance against pathogenic microorganisms such as African swine fever virus, thereby meeting the sterilization and disinfection needs of farms.

[0031] In the present invention, Aspidopterys obcordata Hemsl. is a stem vine of the genus Aspidopterys of the Malpighiaceae family, a woody vine plant distributed in Guangdong, Guangxi, Hainan, Guizhou, Yunnan and other provinces in China, and often grows in sparse forests or shrubs in mountains or valleys at an altitude of 600-1600 meters.

[0032] In some specific embodiments, the hypochlorous acid composite disinfectant comprises the following components in parts by weight:

[0033] 71 parts of hypochlorous acid aqueous solution, 5.5 parts of scutellaria baicalensis extract, 20 parts of polyethylene glycol, 2.5 parts of surfactant and 1 part of phosphate.

[0034] In some specific embodiments, the preparation method of the extract of the Herba Lycopodii var.

[0035] The scutellaria baicalensis is crushed, and then added into water for reflux extraction to obtain an extract;

[0036] The extract is concentrated and dried to obtain the Herba Lycopodii var.

[0037] In some specific embodiments, the working condition parameters of the reflux extraction include: temperature of 100-110° C., time of 1-3 hours, and number of times of 1-3 times.

[0038] In some specific embodiments, the weight ratio of the water to the Myrica ovata is (5-10):1.

[0039] In some specific embodiments, the concentration of the hypochlorous acid aqueous solution is 50 mg / L to 100 mg / L.

[0040] In some specific embodiments, the surfactant includes monoethanolamine lauryl sulfate; and the phosphate includes at least one of sodium phosphate and disodium hydrogen phosphate.

[0041] In some specific embodiments, the polyethylene glycol includes at least one of polyethylene glycol 400 and polyethylene glycol 600.

[0042] In the second aspect, based on the same inventive concept, the present invention provides a method for preparing the hypochlorous acid composite disinfectant according to any one of the first aspects, the method for preparing the hypochlorous acid composite disinfectant comprising the following steps:

[0043] The hypochlorous acid aqueous solution, polyethylene glycol, a surfactant and a phosphate are stirred and mixed to obtain a mixture;

[0044] The mixed material and the extract of the Herba Lycopodii are mixed by ultrasonic stirring to obtain the hypochlorous acid composite disinfectant.

[0045] In some specific embodiments, the working condition parameters of the ultrasonic stirring and mixing include: ultrasonic power of 300-500W, stirring speed of 100-200rpm, and stirring time of 5-15 minutes.

[0046] It should be noted that, for the hypochlorous acid composite disinfectant provided by the present invention and the component raw materials involved in its application, unless otherwise specified or explained, each component can be directly made from commercially available products or can be prepared in-house according to the preparation method disclosed in the prior art; the operation steps involved, unless otherwise specified or explained, can be carried out in accordance with the operation method disclosed in the prior art or using existing equipment according to its use method.

[0047] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0048] Example 1

[0049] This example provides a hypochlorous acid composite disinfectant, which includes the following components in parts by weight:

[0050] 71 parts of hypochlorous acid aqueous solution, 5.5 parts of scutellaria baicalensis extract, 20 parts of polyethylene glycol, 2.5 parts of surfactant and 1 part of phosphate;

[0051] The concentration of the hypochlorous acid aqueous solution is 80 mg / L;

[0052] The polyethylene glycol is polyethylene glycol 400;

[0053] The surfactant is monoethanolamine lauryl sulfate;

[0054] The phosphate is sodium phosphate;

[0055] The preparation method of the extract of the Herba Lycopersicon esculentum comprises the following processes: crushing the Herba Lycopersicon esculentum, adding water accounting for 8 times the weight of the Herba Lycopersicon esculentum for reflux extraction at a temperature of 105°C for 2 hours for 2 times to obtain an extract; concentrating and drying the extract to obtain the Herba Lycopersicon esculentum extract.

[0056] The preparation method of the above-mentioned hypochlorous acid composite disinfectant comprises the following steps:

[0057] The hypochlorous acid aqueous solution, polyethylene glycol, a surfactant and a phosphate are stirred and mixed at a stirring speed of 180 rpm for 10 minutes to obtain a mixture;

[0058] The mixed material and the extract of the Herba Lycopodii are mixed by ultrasonic stirring, with an ultrasonic power of 400 W, a stirring speed of 200 rpm, and a stirring time of 10 minutes to obtain the hypochlorous acid composite disinfectant.

[0059] Example 2

[0060] This example provides a hypochlorous acid composite disinfectant, which includes the following components in parts by weight:

[0061] 60 parts of hypochlorous acid aqueous solution, 8 parts of scutellaria baicalensis extract, 25 parts of polyethylene glycol, 5 parts of surfactant and 2 parts of phosphate;

[0062] The concentration of the hypochlorous acid aqueous solution is 100 mg / L;

[0063] The polyethylene glycol is polyethylene glycol 600;

[0064] The surfactant is monoethanolamine lauryl sulfate;

[0065] The phosphate is disodium hydrogen phosphate;

[0066] The preparation method of the extract of the Herba Lycopersicon esculentum comprises the following processes: crushing the Herba Lycopersicon esculentum, adding water accounting for 8 times the weight of the Herba Lycopersicon esculentum for reflux extraction at a temperature of 105°C for 2 hours for 2 times to obtain an extract; concentrating and drying the extract to obtain the Herba Lycopersicon esculentum extract.

[0067] The preparation method of the above hypochlorous acid composite disinfectant is the same as that in Example 1.

[0068] Example 3

[0069] This example provides a hypochlorous acid composite disinfectant, which includes the following components in parts by weight:

[0070] 75 parts of hypochlorous acid aqueous solution, 3 parts of scutellaria baicalensis extract, 19.5 parts of polyethylene glycol, 2 parts of surfactant and 0.5 parts of phosphate;

[0071] The concentration of the hypochlorous acid aqueous solution is 50 mg / L;

[0072] The polyethylene glycol is polyethylene glycol 600;

[0073] The surfactant is monoethanolamine lauryl sulfate;

[0074] The phosphate is disodium hydrogen phosphate;

[0075] The preparation method of the extract of the Herba Lycopersicon esculentum comprises the following processes: crushing the Herba Lycopersicon esculentum, adding water accounting for 8 times the weight of the Herba Lycopersicon esculentum for reflux extraction at a temperature of 105°C for 2 hours for 2 times to obtain an extract; concentrating and drying the extract to obtain the Herba Lycopersicon esculentum extract.

[0076] The preparation method of the above hypochlorous acid composite disinfectant is the same as that in Example 1.

[0077] Comparative Example 1

[0078] This example provides a disinfectant, which includes the following components in parts by weight:

[0079] 71 parts of hypochlorous acid aqueous solution, 25.5 parts of polyethylene glycol, 2.5 parts of surfactant and 1 part of phosphate;

[0080] The concentration of the hypochlorous acid aqueous solution is 80 mg / L;

[0081] The polyethylene glycol is polyethylene glycol 400;

[0082] The surfactant is monoethanolamine lauryl sulfate;

[0083] The phosphate is sodium phosphate.

[0084] The preparation method of the above disinfectant comprises the following steps:

[0085] The hypochlorous acid aqueous solution, polyethylene glycol, a surfactant and a phosphate are stirred and mixed at a stirring speed of 180 rpm for 10 minutes to obtain a mixture;

[0086] The mixed material is subjected to ultrasonic stirring and mixing, with an ultrasonic power of 400 W, a stirring speed of 200 rpm, and a stirring time of 10 minutes to obtain the disinfectant.

[0087] Comparative Example 2

[0088] This example provides a disinfectant, which includes the following components in parts by weight:

[0089] 71 parts of water, 5.5 parts of scutellaria baicalensis extract, 20 parts of polyethylene glycol, 2.5 parts of surfactant and 1 part of phosphate;

[0090] The polyethylene glycol is polyethylene glycol 400;

[0091] The surfactant is monoethanolamine lauryl sulfate;

[0092] The phosphate is sodium phosphate.

[0093] The preparation method of the above disinfectant comprises the following steps:

[0094] The water, polyethylene glycol, surfactant and phosphate are stirred and mixed at a stirring speed of 180 rpm for 10 minutes to obtain a mixture;

[0095] The mixed material is subjected to ultrasonic stirring and mixing, with an ultrasonic power of 400 W, a stirring speed of 200 rpm, and a stirring time of 10 minutes to obtain the disinfectant.

[0096] Test Case

[0097] This example refers to the test method disclosed in the prior art to evaluate and compare the disinfectants obtained in Examples 1 to 3 and Comparative Examples 1 to 2 above in terms of their inactivation effects on African swine fever virus after conducting accelerated stability tests.

[0098] The test method includes the following steps:

[0099] 1) Test sample pretreatment: According to the Technical Specification for Disinfection 2002 Edition, the disinfectants obtained in Examples 1 to 3 and Comparative Examples 1 to 2 were subjected to accelerated stability tests, and stored at a constant temperature and humidity of 54° C. for 14 days to obtain the test samples after high temperature pretreatment.

[0100] 2) African swine fever virus inactivation effect test:

[0101] 2.1) Test materials: African swine fever virus: The highly pathogenic African swine fever virus Pig / HLJ / 2018 strain was used as the backbone to construct an infectious cloned virus strain carrying a GFP tag; Porcine primary alveolar macrophages: Porcine primary alveolar macrophages were isolated from 30-40 day old SPF pigs, which were sourced from the experimental animal base of Harbin Veterinary Research Institute. PAMs were cultured in 5% CO2 at 37°C using 1640 medium containing 10% fetal bovine serum; Preparation of neutralizer: According to the composition of 0.5% tryptone, 0.25% yeast extract, 1% D-glucose, 0.1% sodium thioglycolate, 0.6% sodium thiosulfate, 0.25% sodium bisulfite, 0.7% lecithin, and 0.5% Tween 80, it was prepared using PBS solution, completely dissolved and pressurized for later use.

[0102] 2.2) Evaluation of simulated immersion disinfection method 1) Virus killing effect of the sample-disinfectant after medium-high temperature pretreatment: Virus concentration 1×10 6Evenly mix 100 copies / mL with disinfectant at a ratio of 1:9, and act at 25℃ for 5 minutes; then add neutralizer at 25℃ for 10 minutes, add cell maintenance solution for 10-fold dilution, add to the monolayer PAMs cell culture plate, repeat 4 wells for each concentration, culture in a 37℃, 5% CO2 incubator, observe the cell status and fluorescence produced by virus replication every 24 hours, and count the test results after 72 hours to see if there is any obvious fluorescence signal. If there is no obvious fluorescence signal, it proves that the African swine fever virus is completely inactivated.

[0103] The test results are shown in Table 1.

[0104] Table 1

[0105]

[0106] It can be seen from Table 1 that, compared with Comparative Examples 1-2, the hypochlorous acid composite disinfectant provided by the embodiment of the present invention still maintains high-efficiency bactericidal performance after high-temperature pretreatment, and can completely inactivate African swine fever virus after 5 minutes of action.

[0107] In summary, the present invention provides a hypochlorous acid composite disinfectant and a preparation method thereof. The present invention mainly uses hypochlorous acid and introduces an appropriate proportion of the extract of the Herba Lycopodii as a disinfectant ingredient, which significantly enhances the overall disinfection effect of the obtained disinfectant. At the same time, it is combined with other components such as polyethylene glycol and phosphate to significantly enhance the stability of the obtained disinfectant, and has efficient bactericidal performance against pathogenic microorganisms such as African swine fever virus, which can meet the sterilization and disinfection needs of farms.

[0108] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present invention; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0109] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A hypochlorous acid composite disinfectant, characterized in that: The hypochlorous acid composite disinfectant comprises the following components in parts by weight: 60-75 parts of hypochlorous acid aqueous solution, 3-8 parts of scutellaria baicalensis extract, 19.5-25 parts of polyethylene glycol, 2-5 parts of surfactant and 0.5-2 parts of phosphate; The preparation method of the extract of the Herba Lycopodii var. The scutellaria baicalensis is crushed, and then added into water for reflux extraction to obtain an extract; The extract is concentrated and dried to obtain the Herba Lycopodii var.

2. The hypochlorous acid composite disinfectant according to claim 1, characterized in that: The hypochlorous acid composite disinfectant comprises the following components in parts by weight: 71 parts of hypochlorous acid aqueous solution, 5.5 parts of scutellaria baicalensis extract, 20 parts of polyethylene glycol, 2.5 parts of surfactant and 1 part of phosphate.

3. The hypochlorous acid composite disinfectant according to claim 1, characterized in that: The working condition parameters of the reflux extraction include: temperature of 100-110° C., time of 1-3 hours, and number of times of 1-3 times.

4. The hypochlorous acid composite disinfectant according to claim 1, characterized in that: The weight ratio of the water to the Herba Lycopodii is (5-10):

1.

5. The hypochlorous acid composite disinfectant according to claim 1, characterized in that: The concentration of the hypochlorous acid aqueous solution is 50 mg / L to 100 mg / L.

6. The hypochlorous acid composite disinfectant according to claim 1, characterized in that: The surfactant includes monoethanolamine lauryl sulfate; and the phosphate includes at least one of sodium phosphate and disodium hydrogen phosphate.

7. The hypochlorous acid composite disinfectant according to claim 1, characterized in that: The polyethylene glycol includes at least one of polyethylene glycol 400 and polyethylene glycol 600.

8. A method for preparing the hypochlorous acid composite disinfectant according to any one of claims 1 to 7, characterized in that: The preparation method of the hypochlorous acid composite disinfectant comprises the following steps: The hypochlorous acid aqueous solution, polyethylene glycol, a surfactant and a phosphate are stirred and mixed to obtain a mixture; The mixed material and the extract of the Herba Lycopodii are mixed by ultrasonic stirring to obtain the hypochlorous acid composite disinfectant.

9. The method for preparing the hypochlorous acid composite disinfectant according to claim 8, characterized in that: The working condition parameters of the ultrasonic stirring and mixing include: ultrasonic power of 300-500W, stirring speed of 100-200rpm, and stirring time of 5-15 minutes.

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