Preparation method of traditional Chinese medicine formula for treating migraine

Abstract

The invention belongs to the technical field of traditional Chinese medicine preparation methods, and particularly relates to a preparation method of a traditional Chinese medicine formula for treating migraine. The method adopts a spray drying method to prepare particles, the drying speed is very high, and the overall production efficiency is improved; the obtained particles are uniform and regular in shape; the method is suitable for most heat-sensitive substances and liquid with high viscosity; particularly, volatile components are reserved to the greatest extent, the quality is stable, the loss of active components is less, and the drug effect is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine preparation methods, and specifically relates to a method for preparing a traditional Chinese medicine formula for treating migraine. Background Art

[0002] Migraine is a common primary headache in clinical practice, characterized by paroxysmal, mostly unilateral, moderate to severe, throbbing headaches. Migraine has a serious impact on the degree of disability, direct and indirect medical expenses, daily work and life, and social burden. However, migraine has long been neglected by the medical community. Whenever severe pain occurs, self-administering painkillers such as acetaminophen and ibuprofen is the only choice for most patients, and a large number of patients abuse painkillers.

[0003] With the gradual development of traditional Chinese medicine, there is a traditional Chinese medicine for treating migraine, which is composed of burdock seeds, chuanxiong, angelica dahurica, yanhusuo, fangfeng, shouwu teng, niuxi, white peony root, licorice, chrysanthemum, cyperus, and kudzu root. This medicine can treat migraine, but the traditional preparation method is currently used, which limits the efficacy of the medicine to a certain extent and has room for improvement. It is now necessary to develop a new preparation method to further improve the treatment effect. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a method for preparing a Chinese medicine formula for treating migraine; the efficacy of the Chinese medicine prepared by the preparation method is greatly improved.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions.

[0006] A method for preparing a Chinese medicine formula for treating migraine comprises the following steps:

[0007] S1. Raw material preparation: collect the raw materials, clean and dry them, crush them with a crusher, screen the crushed raw materials with a rotary vibrating screen, and then mix them according to the proportion;

[0008] S2, raw material preparation: after mixing the raw herbs in S1, use a pressing machine to compress them into granules;

[0009] S3, finished product: The water is removed by spray drying to obtain granules, which are then packaged to make finished products.

[0010] Furthermore, in S1, the raw materials include 6-12 parts of burdock seeds, 3-10 parts of chuanxiong, 3-10 parts of angelica dahurica, 3-10 parts of yanhusuo, 5-10 parts of siler, 9-15 parts of radix polygoni multiflori, 5-12 parts of achyranthes bidentata, 6-15 parts of white peony root, 2-10 parts of licorice, 5-10 parts of chrysanthemum, 6-10 parts of cyperus rotundus, and 10-15 parts of kudzu root.

[0011] Furthermore, in S1, the rotation speed of the pulverizer is 500-1000 rpm, and the rotary vibrating screen uses a 120-130 mesh screen.

[0012] Furthermore, in S3, the spray drying temperature is 60-80° C., and the moisture content of the particles is 1-2%.

[0013] Compared with the prior art, the present invention has the following beneficial effects.

[0014] The traditional Chinese medicine granule preparation method of collecting water for decoction, concentration, obtaining a thick paste, and finally making granules is adopted. It is currently found that the water solubility of the active ingredients of the drug is different, and most of them will be lost by the decoction method. At the same time, the water decoction process will destroy heat-sensitive substances and cause the loss of active oils. Volatile oils are the main ingredients that exert therapeutic effects. On the premise of meeting quality requirements, temperature, humidity, viscosity of the thick paste, etc. will affect the final product, and the quality stability of different batches is still uneven. The preparation process takes a long time. This method uses spray drying to prepare granules, and the drying speed is very fast, which improves the overall production efficiency; the obtained particles are uniform and regular in shape; it is suitable for most heat-sensitive substances and liquids with high viscosity; especially the volatile components are retained to the greatest extent, the quality is stable, the loss of active ingredients is small, and the efficacy is improved.

[0015] The preparation method of the Chinese medicine formula for treating migraine comprehensively examines the triggering mechanism of migraine attacks and the molecular biological abnormalities in the remission period through comparative analysis of detection indicators in peripheral blood and central brain tissue, and in the attack period and remission period, and proposes that the sponge adsorption effect of circNR3C1 on miRNA382-5p is weakened, resulting in enhanced function of CGRP binding and activating its receptor, and overexpression of downstream signaling pathways is a potential pathological mechanism of migraine attacks. By enhancing the sponge adsorption effect of circNR3C1, miRNA382-5p and CGRP are isolated, and the interaction between CGRP and the receptor is blocked, the downstream signaling pathway is called back to prevent and treat migraine attacks. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Bar graph of relative expression levels of CGRP and RAMP1 in the brain.

[0017] Figure 2 Fluorescence staining of CGRP and RAMP1 in the brain (×200).

[0018] Figure 3 Bar graph of the relative expression ratio of p-NMDAR1 / NMDAR1 in the brain.

[0019] Figure 4 Fluorescence staining of p-NMDAR1 and NMDAR1 in the brain (×200). DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0021] A method for preparing a Chinese medicine formula for treating migraine comprises the following steps:

[0022] S1. Raw material preparation: collect the raw materials, clean and dry them, crush them with a crusher, screen the crushed raw materials with a rotary vibrating screen, and then mix them according to the proportion;

[0023] S2, raw material preparation: after mixing the raw herbs in S1, use a pressing machine to compress them into granules;

[0024] S3, finished product: The water is removed by spray drying to obtain granules, which are then packaged to make finished products.

[0025] Furthermore, in S1, the raw materials include 6-12 parts of burdock seeds, 3-10 parts of chuanxiong, 3-10 parts of angelica dahurica, 3-10 parts of yanhusuo, 5-10 parts of siler, 9-15 parts of radix polygoni multiflori, 5-12 parts of achyranthes bidentata, 6-15 parts of white peony root, 2-10 parts of licorice, 5-10 parts of chrysanthemum, 6-10 parts of cyperus rotundus, and 10-15 parts of kudzu root.

[0026] Furthermore, in S1, the rotation speed of the pulverizer is 500-1000 rpm, and the vibrating screen uses a 120-130 mesh screen.

[0027] Furthermore, in S3, the spray drying temperature is 60-80° C., and the moisture content of the particles is 1-2%.

[0028] Example 1.

[0029] A method for preparing a Chinese medicine formula for treating migraine comprises the following steps:

[0030] S1. Raw material preparation: collect the raw materials, clean and dry them, crush them with a crusher, screen the crushed raw materials with a rotary vibrating screen, and then mix them according to the proportion;

[0031] S2. Raw material preparation: Mix the above raw herbs and press them into granules through a press;

[0032] S3, finished product: The water is removed by spray drying to obtain granules, which are then packaged to make finished products.

[0033] In this embodiment, the raw materials specifically include 12 parts of burdock seeds, 10 parts of chuanxiong, 10 parts of angelica dahurica, 10 parts of corydalis yanhusuo, 8 parts of siler, 15 parts of radix polygoni multiflori, 10 parts of achyranthes bidentata, 15 parts of white peony root, 10 parts of liquorice, 10 parts of chrysanthemum, 6 parts of cyperus rotundus, and 10 parts of kudzu root.

[0034] In this embodiment, the rotation speed of the pulverizer in S1 is 650 rpm, and the vibrating screen uses a 120-mesh screen.

[0035] In this embodiment, the spray drying temperature in S3 is 6° C. and the water content is 1%.

[0036] Comparative example 1.

[0037] The comparative example group adopts the raw material composition of the embodiment, and the preparation method is the existing preparation method: add water and boil for three times, add 8 times of water for the first time, boil for 1 hour; add 6 times of water for the second time, boil for 40 minutes; add 6 times of water for the third time, boil for 40 minutes. Combine the filtrate, let it stand for 12 hours, take the supernatant and concentrate it to a thick paste with a relative density of 1.28 to 1.30, add sucrose and starch, mix well, make granules, dry, make whole granules, make 1000g, and pack them.

[0038] The pharmacological experiments were carried out on the Chinese medicine formulas of the embodiments and comparative examples, which specifically comprised the following steps:

[0039] S1. Pain regulation: Experiment on acetic acid-induced writhing reaction in mice

[0040] Male mice weighing 18-22 grams were selected and randomly divided into a model group, a comparative group, and an example group, with 10 mice in each group. The drugs were administered continuously for 3 days, and the model group was given the same volume of water. 45 minutes after the last administration, 0.6% glacial acetic acid 0.1 ml / 10 g was intraperitoneally injected. The number of twisting reactions of the mice within 10 minutes after the administration of glacial acetic acid was observed, and the percentage of analgesia was calculated. The results are shown in Table 1.

[0041] Table 1 Acetic acid-induced writhing reaction pain experiment

[0042]

[0043]

[0044] Note: Compared with the model group ** P<0.01, comparison between Example 1 and Comparative Example 1 # P<0.05

[0045] Both the comparative example and the example preparations exhibited analgesic effects, and the analgesic percentage of the example preparation was higher than that of the comparative example, indicating an improved analgesic effect.

[0046] S2. Establishment and evaluation of migraine model: 40 healthy SD rats, weighing 220±25g, were randomly divided into 4 groups according to their body weight, with 10 rats in each group, namely: Group I-blank control group, Group II-model group, Group III-comparative group, Group IV-implementation group. All animals were placed in a constant temperature, quiet, normal light environment with free access to food and water. After 3 days of adaptation to feeding, rats in groups II, III, and IV were given subcutaneous injections of nitroglycerin (NTG, 5 mg / kg) to establish the model. After the last injection, the rats showed frequent head shaking, scratching their heads with both forelimbs, and scratching their heads with one hind limb. Compared with the irritability and large range of activity before the injection, the migraine-like behavior was considered to be an attack.

[0047] S3. Regulatory effect on blood CGRP: The ELISA method was used to detect the CGRP content in the blood. The results are shown in Table 2.

[0048] Table 2 Comparison of CGRP content in rat blood

[0049] Group Number of tested animals Dosage (g / kg) CGRP (pg / ml) Blank group 10 - <![CDATA[331.06±40.01 ** ]]> Model Group 10 - 447.08±20.27 Comparative group 10 5.5 <![CDATA[364.33±21.69 ** ]]> Example Group 10 5.5 <![CDATA[346.41±17.54 **## ]]>

[0050] Note: Compared with the model group, P < 0.01; compared with the example group and the comparative example group ## P<0.01

[0051] Both the comparative example and the example preparations can reduce the CGRP content in the blood. Compared with the comparative example group, the example preparation has a better reducing effect.

[0052] S4. Regulatory effect on CGRP in brain tissue: The ELISA method was used to detect the CGRP content in the brain. The results are shown in Table 3.

[0053] Table 3 Content of CGRP in brain

[0054] Group Number of tested animals Dosage (g / kg) CGRP (pg / ml) Blank group 10 - <![CDATA[25.39±2.62 ** ]]> Model Group 10 - 50.50±4.77 Comparative group 10 5.5 <![CDATA[36.30±3.39 ** ]]> Example Group 10 5.5 <![CDATA[33.73±4.20 **## ]]>

[0055] Note: Compared with the model group ** P<0.01, comparison between the example group and the comparative example group ## P<0.01

[0056] S5. Regulatory effect on the expression of circNR3C1 and miRNA382-5p in brain tissue: Fluorescence quantitative PCR was used to detect the expression of circNR3C1 and miRNA382-5p in the brain. The results are shown in Table 4.

[0057] Table 4 Expression of circNR3C1 and miRNA382-5p in brain

[0058]

[0059] Note: Compared with the model group** P<0.01, comparison between the example group and the comparative example group ## P<0.01

[0060] S6. Regulatory effect on CGRP and related proteins in the cerebral cortex: Fluorescent immunostaining was used to observe the relative expression of CGRP and RAMP1 in brain tissue, and the ratio of p-NMDAR1 / NMDAR1 relative expression, as shown in Table 5. Figure 1-4 shown.

[0061] Table 5 Relative expression of CGRP, RAMP1, and p-NMDAR1 / NMDAR1 in the brain

[0062]

[0063] Note: Compared with the model group ** P<0.01, comparison between the example group and the comparative example group ## P<0.01

[0064] The present invention comprehensively investigates the triggering mechanism of migraine attacks and the molecular biological abnormalities in the remission period through peripheral blood and central brain tissue, and proposes that the sponge adsorption effect of circNR3C1 on miRNA382-5p is weakened, resulting in enhanced function of CGRP binding and activating its receptor, and overexpression of downstream signaling pathways is a potential pathological mechanism of migraine attacks. By enhancing the sponge adsorption effect of circNR3C1, miRNA382-5p and CGRP are isolated, and the interaction between CGRP and the receptor is blocked, thereby calling back the downstream signaling pathway to prevent and treat migraine attacks, thereby realizing the chronicization of acute patients on the market, the clinical diagnosis of migraine, and the supplement of specific preventive and therapeutic drugs, and is used for the treatment of migraine, cluster headache, tension headache and neuralgic headache.

[0065] At the same time, the contents not described in detail in this specification belong to the prior art known to those skilled in the art. It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0066] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a Chinese medicine formula for treating migraine, characterized in that: The following steps are involved: S1. Raw material preparation: collect the raw materials, clean and dry them, crush them with a crusher, screen the crushed raw materials with a rotary vibrating screen, and then mix them according to the proportion; S2, raw material preparation: after mixing the raw herbs in S1, use a pressing machine to compress them into granules; S3, finished product: The water is removed by spray drying to obtain granules, which are then packaged to make finished products.

2. The method for preparing the Chinese medicine formula for treating migraine according to claim 1, characterized in that: In S1, the raw materials include 6-12 parts of burdock seeds, 3-10 parts of chuanxiong, 3-10 parts of angelica dahurica, 3-10 parts of yanhusuo, 5-10 parts of siler, 9-15 parts of radix polygoni multiflori, 5-12 parts of achyranthes bidentata, 6-15 parts of white peony root, 2-10 parts of liquorice, 5-10 parts of chrysanthemum, 6-10 parts of cyperus rotundus, and 10-15 parts of kudzu root.

3. The method for preparing the Chinese medicine formula for treating migraine according to claim 1, characterized in that: In S1, the rotation speed of the pulverizer is 500-1000 rpm, and the rotary vibrating screen uses a 120-130 mesh screen.

4. The method for preparing the Chinese medicine formula for treating migraine according to claim 1, characterized in that: In S3, the spray drying temperature is 60-80°C, and the moisture content of the particles is 1-2%.

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