Milk powder for promoting growth and development of children and preparation method thereof

Abstract

The invention belongs to the technical field of milk powder formulas, and particularly relates to milk powder for promoting growth and development of children and a preparation method thereof. Comprising the following components: raw milk, whole milk powder, dried skim milk, lactose, isomaltooligosacharide, fructo-oligosaccharide, galactooligosaccharide, colostrum basic protein, whey protein powder, cattle spleen peptide powder, comprehensive fruit powder, hydrolyzed yolk powder, phosphatidylserine, xanthophyll, compound vitamins, compound minerals, choline chloride, L-carnitine, calcium carbonate, milk mineral salt and lactoferrin. The composition is prepared from docosahexaenoic acid grease, plant lactobacillus YBT5 and plant lactobacillus SG5; in the preparation method of the milk powder, a dry-wet mixing method is adopted to obtain the milk powder. According to the formula, reasonable nutrients are selected, a comprehensive and balanced proportion is adopted, nutrition of children can be balanced, growth and development of the children can be promoted, and the milk powder is simple in preparation method, convenient to operate and suitable for large-scale production.

Description

Technical Field

[0001] The invention belongs to the technical field of milk powder formula, and in particular relates to milk powder for promoting children's growth and development and a preparation method thereof. Background Art

[0002] Milk powder is a nutritionally balanced formula food containing protein, calcium, phosphorus, vitamin D, etc. Calcium is an important nutrient element that contributes to the growth of children's bones, so appropriate supplementation of milk powder helps maintain bone health and is beneficial to height growth. In the prior art CN103053691B discloses a children's formula goat milk powder and a preparation method thereof, the children's goat milk powder formula includes the following raw materials in parts by weight: 400-600 parts of fresh goat milk, 20-40 parts of desalted whey powder, 4-10 parts of concentrated whey protein powder, 10-15 parts of low trans fatty acid mixed vegetable oil, 1-6 parts of walnut oil, 2-8 parts of oligofructose, 0.2-0.6 parts of docosahexaenoic acid, 0.4-0.8 parts of eicosatetraenoic acid, 1-5 parts of compound fruit and vegetable powder. , 0.06-0.1 parts of choline, 0.03-0.05 parts of taurine, 0.1-0.5 parts of complex vitamins, and 0.2-0.5 parts of complex trace elements. The formula optimizes seven nutrients required for brain development and vision development, including DHA, ARA, choline, α-linolenic acid, linoleic acid, lutein and taurine, and adds prebiotic oligofructose and walnut oil rich in polyunsaturated fatty acids. The phospholipids in walnut oil are rich, which can increase cell activity and improve children's memory and intelligence.

[0003] Although there are many types of children's formula milk powder on the market, the different formula compositions and content differences have a great impact on the efficacy of promoting children's growth and development, and will cause a large amount of nutrient loss during the production and storage processes, which is not conducive to the stability of product quality. Summary of the invention

[0004] The purpose of the present invention is to provide a milk powder for promoting the growth and development of children and a preparation method thereof, so as to solve the problems existing in the prior art.

[0005] To achieve the above-mentioned object of the invention, the technical solution of the present invention is as follows:

[0006] On the one hand, the present invention provides a milk powder for promoting the growth and development of children, comprising the following components: 55-65 parts of raw cow's milk, 10-14 parts of whole milk powder, 17-21 parts of skim milk powder, 4-6 parts of lactose, 3-4 parts of isomaltooligosaccharide, 0.06-1 part of fructooligosaccharide, 0.06-1 part of galacto-oligosaccharide, 0.08-0.09 part of colostrum alkaline protein, 0.04-0.05 part of whey protein powder, 0.04-0.05 part of bovine spleen peptide powder, 0.045 parts of mixed fruit powder, 0.01-0.02 parts of hydrolyzed egg yolk powder, 0.009-0.014 parts of phosphatidylserine, 0.003 parts of lutein, 0.09 parts of complex vitamins, 0.06-1 parts of complex minerals, 0.04-0.05 parts of choline chloride, 0.004 parts of L-carnitine, 0.4-0.5 parts of calcium carbonate, 0.08-0.09 parts of milk mineral salts, 0.05-0.1 parts of lactoferrin, 0.06 parts of docosahexaenoic acid oil, 0.001 parts of Lactobacillus plantarum YBT5 and 0.001 parts of Lactobacillus plantarum SG5.

[0007] Specifically, the mixed fruit powder includes pineapple powder, banana powder, apple powder, orange powder, peach powder and lemon powder, and the ratio of pineapple powder: banana powder: apple powder: orange powder: peach powder: lemon powder is 1:1:1:1:1:1.

[0008] Specifically, each 100g of the compound vitamin contains 450μg RE vitamin A, 8.2μg vitamin D, 3mg α-TE vitamin E, 20μg vitamin K, 0.71mg vitamin B1, 0.85mg vitamin B2, 0.13mg vitamin B6, 1.61μg vitamin B 12 , 17.5mg vitamin C, 3.51mg vitamin B3, 93μg vitamin B9, 3mg vitamin B5 and 82.5mg vitamin B4.

[0009] Specifically, the compound minerals consist of the following components: magnesium, calcium, iron, zinc and selenium.

[0010] More specifically, the complex mineral contains 35-40 mg magnesium, 1140-1150 mg calcium, 7.0-8.0 mg iron, 6.25-6.5 mg zinc and 7.8-8.0 μg selenium per 100 g.

[0011] In another aspect, the present invention provides a method for preparing milk powder for promoting growth and development of children, comprising the following steps:

[0012] (1) Weigh the core material ingredients according to the formula and mix them evenly;

[0013] (2) Weigh lactose and skim milk powder in a ratio of 1:1-1:4, dissolve at 50-80°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 20-40%, and stir well to obtain a mixed solution;

[0014] (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

[0015] Specifically, the ratio of lactose to skimmed milk powder in step (2) is 1:1-1:3.

[0016] Preferably, the ratio of lactose to skimmed milk powder in step (2) is 1:2.

[0017] Specifically, the dissolving temperature in step (2) is 60-80°C.

[0018] Preferably, the dissolving temperature in step (2) is 70°C.

[0019] Specifically, the total solid concentration in step (2) is 25-40%.

[0020] Preferably, the total solids concentration in step (2) is 35%.

[0021] In yet another aspect, the present invention discloses an application of milk powder in promoting the growth and development of children.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The invention discloses a milk powder for promoting children's growth and development and a preparation method thereof. The formula of the invention selects reasonable basic nutritional elements and adopts a comprehensive and balanced ratio, which can not only balance children's nutrition, increase children's appetite, improve body immunity, promote children's growth and development, but also improve children's bone development. The dry-wet mixing preparation process of the patent not only reduces the loss of ingredients and nutrients, but also improves the mixing uniformity of the product, and is suitable for large-scale production. DETAILED DESCRIPTION

[0024] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples without specifying specific conditions are usually selected according to conventional methods and conditions or according to the product specifications.

[0025] Experimental Materials:

[0026] Lactobacillus plantarum YBT5 (purchased from Harbin Ankefu Biotechnology Co., Ltd.) and Lactobacillus plantarum SG5 (purchased from Suzhou Shanenkang Biotechnology Co., Ltd.).

[0027] Example 1 A milk powder formula for promoting children's growth and development

[0028] Core ingredients: 3.6 parts of isomaltooligosaccharide, 0.09 parts of fructooligosaccharide, 0.09 parts of galacto-oligosaccharide, 0.08 parts of colostrum alkaline protein, 0.045 parts of whey protein powder, 0.045 parts of bovine spleen peptide powder, 0.045 parts of mixed fruit powder, 0.02 parts of hydrolyzed egg yolk powder, 0.009 parts of phosphatidylserine, 0.003 parts of lutein, 0.09 parts of compound vitamins, 0.09 parts of compound minerals, 0.045 parts of choline chloride, 0.004 parts of L-carnitine;

[0029] Remaining ingredients: 60 parts of raw milk, 12 parts of whole milk powder, 19 parts of skim milk powder, 5 parts of lactose, 0.5 parts of calcium carbonate, 0.09 parts of milk mineral salts, 0.1 parts of lactoferrin, 0.06 parts of docosahexaenoic acid oil, 0.001 parts of Lactobacillus plantarum YBT5 and 0.001 parts of Lactobacillus plantarum SG5;

[0030] Among them, each 100g of compound vitamins contains 450μg RE vitamin A, 8.2μg vitamin D, 3mg α-TE vitamin E, 20μg vitamin K, 0.71mg vitamin B1, 0.85mg vitamin B2, 0.13mg vitamin B6, 1.61μg vitamin B 12 , 17.5mg vitamin C, 3.51mg vitamin B3, 93μg vitamin B9, 3mg vitamin B5 and 82.5mg vitamin B4;

[0031] Among them, every 100g of complex minerals contains 40mg magnesium, 1150mg calcium, 8.0mg iron, 6.25mg zinc and 8.0μg selenium.

[0032] Example 2 A milk powder formula for promoting children's growth and development

[0033] Compared with Example 1, the difference is that the mass fractions of the following components are changed, and the other components remain unchanged. Core material: 0.09 parts of colostrum alkaline protein, 0.01 parts of hydrolyzed egg yolk powder, 0.014 parts of phosphatidylserine Remaining ingredients: 0.08 parts of milk mineral salt

[0034] Effect Example 1 Functional Testing of Milk Powder

[0035] Experimental samples: basic formula milk powder, control group milk powder (Example 2), and patented milk powder (Example 1), for experimental use;

[0036] Experimental animals: SPF healthy male SD rats (14 days old), average weight 29.39±1.89 g, provided by Changsha Tianqin Biotechnology Co., Ltd., production license number SCXK (Xiang) 2019-0014, certificate number: 4307262011001866;

[0037] Experimental conditions: The experimental animals were kept in the SPF animal laboratory of Xiangya School of Public Health, Central South University, with a temperature of 21-23°C and a relative humidity of 50%-60%;

[0038] Experimental reagents and instruments: rat bone alkaline phosphatase (BAP) ELISA kit, rat type I procollagen amino-terminal peptide (PINP) ELISA kit, rat osteocalcin (BGP) ELISA kit, rat 25-hydroxyvitamin D (25-OH-VD) ELISA kit, CS-T300 automatic biochemical analyzer (Changchun Dirui Industrial Co., Ltd.), H1650R desktop high-speed refrigerated centrifuge (Xiangyi Power Testing Instrument Co., Ltd.), MB-530 multifunctional enzyme label analyzer (Shenzhen Huisong Technology Development Co., Ltd., LC-20A liquid chromatograph (Shimadzu);

[0039] Experimental grouping: 14-day-old SD rats were randomly divided into 4 groups, namely, the basic formula milk powder group (blank group), the control milk powder group (Example 2), the low-dose milk powder group of the patent (1.2 g / d), and the high-dose milk powder group of the patent (3.6 g / d), with 12 rats in each group. During the experiment, the animals in each group were fed with milk and feed according to the nutritional requirements of mice and rats, and were free to drink water in the later stage. The experimental period was 4 weeks;

[0040] Statistical methods: Mean ± standard deviation was used for data processing using SPSS18.00 software package. If the data showed a normal distribution and the variances between the groups were equal, one-way analysis of variance was used, and P ≤ 0.05 was considered statistically significant.

[0041] 1.1 Effects of milk powder on serum parameters of rats

[0042] 1.1.1 Effect of milk powder on serum 25-hydroxyvitamin D in rats

[0043] The above-mentioned corresponding kit was used to measure the serum 25-hydroxyvitamin D content of rats fed for 14 days and 28 days. The results are shown in Table 1:

[0044] Table 1 Serum 25-hydroxyvitamin D content in rats

[0045]

[0046]

[0047] 1.1.2 Effect of milk powder on serum osteocalcin in rats

[0048] The above-mentioned corresponding kit was used to measure the serum osteocalcin content of rats fed for 14 days and 28 days. The results are shown in Table 2 below:

[0049] Table 2 Serum osteocalcin content of rats

[0050]

[0051] 1.1.3 Effect of milk powder on serum bone alkaline phosphatase in rats

[0052] The above-mentioned corresponding kit was used to measure the content of bone alkaline phosphatase in rats fed for 14 days and 28 days. The results are shown in Table 3 below:

[0053] Table 3 Content of bone alkaline phosphatase in rats

[0054]

[0055] 1.1.4 Effect of milk powder on serum type Ⅰ procollagen amino-terminal peptide in rats

[0056] The above-mentioned corresponding kit was used to measure the content of type I procollagen amino terminal peptide in rats fed for 14 days and 28 days. The results are shown in Table 4 below:

[0057] Table 4 Content of amino-terminal peptide of type Ⅰ procollagen in rats

[0058]

[0059]

[0060] 1.2 Effect of milk powder on bone density in rats

[0061] 1.2.1 Effect of milk powder on femoral bone density in rats

[0062] The bone density of the femur of rats fed for 14 days and 28 days was measured using a dual-energy X-ray absorptiometry instrument. The results are shown in Table 5:

[0063] Table 5 Femoral bone density of rats

[0064]

[0065] 1.2.2 Effect of milk powder on tibia bone density in rats

[0066] The bone density of the tibia of rats fed for 14 days and 28 days was measured using a dual-energy X-ray absorptiometry instrument. The results are shown in Table 6:

[0067] Table 6 Tibia bone density of rats

[0068]

[0069] 1.3 Effect of milk powder on bone length in rats

[0070] 1.3.1 Effect of milk powder on femur length in rats

[0071] The femur lengths of rats fed for 14 days and 28 days were measured using a vernier caliper. The results are shown in Table 7 below:

[0072] Table 7 Femur length of rats

[0073]

[0074]

[0075] 1.3.2 Effect of milk powder on tibia length in rats

[0076] Tibia length of rats fed for 14 days and 28 days was measured using a vernier caliper. The results are shown in Table 8 below:

[0077] Table 8 Tibia length of rats

[0078]

[0079] Based on the experimental results in Tables 1-8 above, the patented milk powder can increase the content of 25-hydroxyvitamin D, osteocalcin, bone alkaline phosphatase and type I procollagen amino-terminal peptide in rat serum, and can promote the increase of tibia bone density and femur length in rats, and the effect is better than other formula ratios.

[0080] Example 3 A method for preparing milk powder

[0081] (1) Weigh the core material ingredients according to Example 1 and mix them evenly;

[0082] (2) Weigh lactose and skim milk powder in a ratio of 1:2, dissolve at 70°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 35%, and stir well to obtain a mixed solution;

[0083] (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

[0084] Embodiment 4 A method for preparing milk powder

[0085] (1) Weigh the core material ingredients according to Example 1 and mix them evenly;

[0086] (2) Weigh lactose and skim milk powder in a ratio of 1:1, dissolve at 70°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 35%, and stir well to obtain a mixed solution;

[0087] (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

[0088] Example 5 A method for preparing milk powder

[0089] (1) Weigh the core material ingredients according to Example 1 and mix them evenly;

[0090] (2) Weigh lactose and skim milk powder in a ratio of 1:2, dissolve at 70°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 30%, and stir well to obtain a mixed solution;

[0091] (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

[0092] Example 6 A method for preparing milk powder

[0093] (1) Weigh the core material ingredients according to Example 1 and mix them evenly;

[0094] (2) Weigh lactose and skim milk powder in a ratio of 1:2, dissolve at 60°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 35%, and stir well to obtain a mixed solution;

[0095] (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

[0096] Example 7 A method for preparing milk powder

[0097] (1) Weigh the core material ingredients according to Example 1 and mix them evenly;

[0098] (2) Weigh lactose and skim milk powder in a ratio of 1:2, dissolve at 80°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 35%, and stir well to obtain a mixed solution;

[0099] (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

[0100] Comparative Example 1 A method for preparing milk powder by dry-wet compounding

[0101] The prior art dry-wet composite group: raw milk, skim milk powder, whole milk powder, lactose, isomaltooligosaccharide, oligofructose, oligogalactose, milk mineral salts, colostrum alkaline protein, whey protein powder (containing lysine), bovine spleen peptide powder, mixed fruit powder (pineapple powder, banana powder, apple powder, orange powder, peach powder, lemon powder), hydrolyzed egg yolk powder, phosphatidylserine (PS), vitamin K2, lutein (5%), compound vitamins, compound minerals, calcium carbonate, choline chloride, L-carnitine are dissolved in water at a mass ratio of 1:4, stirred evenly and then spray-dried, and then mixed evenly with Lactobacillus plantarum YBT5, Lactobacillus plantarum SG5, lactoferrin, and docosahexaenoic acid oil (10% powder) to obtain milk powder.

[0102] Effect Example 2: Detection of the stability of nutrients in milk powder

[0103] The milk powder of this patent (Example 1) was produced by the preparation methods of Examples 3-7 and Comparative Example 1, and the obtained milk powder was stored in a storage room at a temperature of 25° C. and a relative humidity of ≤60%. The test period was 2 months. The vitamin content in the sample was detected on the 0th day of storage and after 2 months of storage.

[0104] Detection method: Vitamin A (GB 5009.826 first method), Vitamin D (GB 5009.82 fourth method), Vitamin E (GB 5009.82 first method), Vitamin K (GB 5009.158 first method), Vitamin B1 (GB 5009.84 first method), Vitamin B2 (GB 5009.85 first method), Vitamin B6 (GB 5009.154 first method), Vitamin B12 (GB 5009.285 third method), Vitamin C (GB 5413.18), Niacin (GB 5009.89 second method), Folic acid (GB 5009.211), Pantothenic acid (GB5009.210 first method), Choline (GB 5413.20 first method). The nutrient element loss rate was calculated based on the test values, and the formula is as follows: Loss rate = (test value of the sample on day 0 - test value of the sample after 2 months of storage) / test value of the sample on day 0 × 100%.

[0105] The test results are shown in Table 9 below. Within the storage period of 2 months, the vitamin loss rate of Comparative Example 1 is between 0 and 9.02%; the vitamin loss rate of the preparation method of the present patent (Example 3) is significantly reduced, between 0 and 4.98%, which indicates that the protective film formed by the lactose and skim milk powder through the technical solution effectively protects the nutrients and reduces their loss rate; Examples 4-7 show that after changing the ratio of lactose and skim milk powder, the dissolution temperature, and the total solid concentration, the implementation effect of the present technical solution cannot be achieved.

[0106] Table 9 Storage stability results of each group (%)

[0107] project Comparative Example 1 Example 3 Example 4 Example 5 Example 6 Example 7 Vitamin A 7.33 3.85 7.05 6.18 4.82 6.84 Vitamin D 6.04 3.99 6.60 4.70 5.52 4.51 Vitamin E 3.31 1.02 2.86 3.47 3.42 3.68 Vitamin K 4.36 1.01 3.73 2.04 4.73 3.17 <![CDATA[Vitamin B1]]> 5.32 2.22 5.12 4.24 5.94 3.50 <![CDATA[Vitamin B2]]> 0 0 0 0 0 0 <![CDATA[Vitamin B6]]> 7.98 2.11 5.28 5.05 4.08 4.17 <![CDATA[Vitamin B 12 > 8.55 1.02 4.03 2.29 8.37 4.61 Vitamin C 9.02 4.98 5.38 9.34 6.49 6.99 niacin 0 0 0 0 0 0 Folic acid 6.43 2.10 5.34 4.17 6.49 4.53 Pantothenic acid 7.31 2.09 5.78 3.72 3.20 6.30 choline 4.29 1.58 2.60 3.53 2.38 4.27

Claims

1. A milk powder for promoting growth and development of children, characterized in that: The milk powder is composed of the following components: 55-65 parts of raw milk, 10-14 parts of whole milk powder, 17-21 parts of skim milk powder, 4-6 parts of lactose, 3-4 parts of isomaltooligosaccharide, 0.06-1 parts of fructooligosaccharide, 0.06-1 parts of galacto-oligosaccharide, 0.08-0.09 parts of colostrum alkaline protein, 0.04-0.05 parts of whey protein powder, 0.04-0.05 parts of bovine spleen peptide powder, 0.045 parts of mixed fruit powder, 0.01-0.02 parts of hydrolyzed egg yolk powder, 0. 009-0.014 parts of phosphatidylserine, 0.003 parts of lutein, 0.09 parts of complex vitamins, 0.06-1 parts of complex minerals, 0.04-0.05 parts of choline chloride, 0.004 parts of L-carnitine, 0.4-0.5 parts of calcium carbonate, 0.08-0.09 parts of milk mineral salts, 0.05-0.1 parts of lactoferrin, 0.06 parts of docosahexaenoic acid oil, 0.001 parts of Lactobacillus plantarum YBT5 and 0.001 parts of Lactobacillus plantarum SG5.

2. The milk powder according to claim 1, characterized in that The comprehensive fruit powder comprises pineapple powder, banana powder, apple powder, orange powder, peach powder and lemon powder, and the ratio of pineapple powder: banana powder: apple powder: orange powder: peach powder: lemon powder is 1:1:1:1:1:

1.

3. The milk powder according to claim 1, characterized in that The compound vitamin contains 450 μg RE vitamin A, 8.2 μg vitamin D, 3 mg α-TE vitamin E, 20 μg vitamin K, 0.71 mg vitamin B1, 0.85 mg vitamin B2, 0.13 mg vitamin B6, 1.61 μg vitamin B 12 , 17.5mg vitamin C, 3.51mg vitamin B3, 93μg vitamin B9, 3mg vitamin B5 and 82.5mg vitamin B4.

4. The milk powder according to claim 1, characterized in that The compound minerals consist of the following components: magnesium, calcium, iron, zinc and selenium.

5. The milk powder according to claim 4, characterized in that Every 100g of the compound mineral contains 35-40mg magnesium, 1140-1150mg calcium, 7.0-8.0mg iron, 6.25-6.5mg zinc and 7.8-8.0μg selenium.

6. The method for preparing milk powder according to claim 1, characterized in that: The following steps are involved: (1) Weigh the core material ingredients according to the formula and mix them evenly; (2) Weigh lactose and skim milk powder in a ratio of 1:1-1:4, dissolve at 50-80°C and mix well. After the temperature drops to room temperature, add the core material, control the total solid concentration to 20-40%, and stir well to obtain a mixed solution; (3) After the mixed solution obtained in step (2) is spray-dried, the remaining ingredients of the formula are added and mixed evenly to obtain milk powder.

7. The preparation method according to claim 6, characterized in that: The ratio of lactose to skimmed milk powder in step (2) is 1:1-1:

3.

8. The preparation method according to claim 6, characterized in that: The dissolving temperature in step (2) is 60-80°C.

9. The preparation method according to claim 6, characterized in that: The total solid concentration in step (2) is 25-40%.

10. Use of the milk powder according to claim 1 in promoting the growth and development of children.

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