Nutrient solution containing chlorella as well as preparation method, application and use method of nutrient solution

Abstract

The invention relates to the technical field of livestock feed, in particular to a nutrient solution containing chlorella as well as a preparation method, application and a use method of the nutrient solution. The nutrient solution comprises a chlorella product, a nutrition additive and a rumen fermentation regulator, and the ratio of the chlorella product to the nutrition additive to the rumen fermentation regulator is 100 mL: (5-10) g: (20-35) g. The chlorella product is prepared from chlorella enzymatic hydrolysate and lactobacillus plantarum fermentation liquor. The nutrition additive comprises vitamins and trace elements; the rumen fermentation regulator comprises sodium bicarbonate and chitosan. The invention also provides an application of the nutrient solution in barn-feeding beef cattle breeding and a preparation and use method of the nutrient solution. The prepared nutrient solution containing chlorella not only can improve the immunity of barn-feeding beef cattle, but also can reduce fat deposition of the beef cattle and improve the beef quality.

Description

Technical Field

[0001] The invention belongs to the technical field of livestock feed, and in particular relates to a nutrient solution containing chlorella and a preparation method, application and use method thereof. Background Art

[0003] Although barn-feeding beef cattle has advantages such as increasing growth rate, improving daily ration rewards and reducing grassland carrying capacity, it also has some disadvantages that cannot be ignored. The most prominent problem is that the high-concentrate diet condition increases the intramuscular fat content of beef cattle, which on the one hand leads to health risks for beef cattle themselves (decreased immunity) and the occurrence of nutritional metabolic diseases such as fatty liver, and on the other hand reduces the edible value of beef (decreased meat quality). At the same time, barn-feeding beef cattle also generally have disadvantages such as decreased feed intake and poor production performance.

[0004] From the perspective of enhancing immunity, improving meat quality and increasing feed intake, the current nutritional measures taken in the production of house-fed beef cattle mainly include: adding enzyme preparations, α-linolenic acid, organic acids, Chinese herbal medicine additives, trace elements and vitamin additives, plant extracts, chitosan and microecological preparations to their diets. Among the above technologies, enzyme preparations, organic acids, chitosan and microecological preparations are easily affected by the fermentation characteristics of the rumen of beef cattle, and the effect is not very stable; the application effect of Chinese herbal medicine additives is easily affected by the raw materials, compatibility and processing technology, and the price is relatively expensive; trace elements and vitamin additives are only considered from the perspective of trace elements and vitamin nutrition of beef cattle. Under normal circumstances, it is not easy to lack these two nutrients in the mixed diet of beef cattle; α-linolenic acid needs to solve the problem of passing through the rumen, otherwise the effect is very general; plant extracts are also easily affected by their sources, extraction processes, dosages, etc., so the application of such technologies in the actual production of house-fed beef cattle is also very limited.

[0005] Therefore, there is an urgent need to improve the existing nutritional measures for house-raised beef cattle and provide a nutrient solution to solve the problems of decreased meat quality and reduced immunity in the production of house-raised beef cattle. Summary of the invention

[0006] In order to solve the technical problems of reduced meat quality and lowered immunity of house-raised beef in the prior art, the present invention provides a nutrient solution containing Chlorella and a preparation method, application and use method thereof.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect, the present invention provides a nutrient solution containing Chlorella, comprising a Chlorella product, a nutrient additive and a rumen fermentation regulator, wherein the ratio of the Chlorella product, the nutrient additive and the rumen fermentation regulator is 100 mL: (5-10) g: (20-35) g; the Chlorella product is prepared from Chlorella hydrolysate and Lactobacillus plantarum fermentation liquid; the nutrient additive comprises vitamins and trace elements; and the rumen fermentation regulator comprises sodium bicarbonate and chitosan.

[0009] Preferably, the Chlorella product is obtained by mixing Chlorella hydrolysate and Lactobacillus plantarum fermentation broth in a volume ratio of 5:(1-3) and subjecting the mixture to low-pressure homogenization for 10-15 minutes, wherein the low-pressure homogenization is carried out at 20-40 MPa.

[0010] Preferably, the preparation process of the Chlorella hydrolysate is as follows: 100 mL of Chlorella culture solution is taken, and high-pressure homogenization is performed for 10 minutes under 80-100 MPa conditions to obtain a Chlorella cell homogenate, 10-20 g of wheat gluten is added, and the mixture is stirred for 5 minutes to obtain a mixed solution, and alkaline protease accounting for 2-8% of the mass of the mixed solution is added, and the pH value is adjusted to 8-10, and ultrasonic-assisted enzymolysis is performed in a water bath at 40-55° C. for 40-60 minutes to obtain an enzymolysate.

[0011] Preferably, the plant lactobacillus fermentation broth is prepared according to the following steps: adding chlorella culture broth to MRS liquid culture medium and sterilizing at high temperature, inoculating plant lactobacillus suspension after standing at room temperature, and fermenting at 37° C. for 8 to 12 hours to obtain fermentation broth, wherein the volume ratio of the chlorella culture broth, the MRS liquid culture medium and the plant lactobacillus suspension is (0.1 to 0.3):1:0.08.

[0012] Preferably, the vitamins include niacin, biotin and folic acid, and the trace elements include selenium, zinc, iron, copper, manganese, iodine and cobalt.

[0013] Preferably, based on the total mass of the nutritional additive as 100%, the nutritional additive includes the following components in percentage by mass: 35-45% niacin, 1-3% biotin, 5-9% folic acid, 0.1-0.7% selenium, 11-14% zinc, 22-32% iron, 1-3% copper, 6-10% manganese, 0.1-0.5% iodine, and 0.1-0.5% cobalt.

[0014] Preferably, the mass ratio of sodium bicarbonate to chitosan in the rumen fermentation regulator is 100:(1-10).

[0015] In a second aspect, the present invention provides a method for preparing a nutrient solution containing Chlorella, comprising the following steps: adding a nutrient additive and a rumen fermentation regulator to the Chlorella product in proportion and mixing to obtain a nutrient solution containing Chlorella.

[0016] In a third aspect, the present invention provides an application of a nutrient solution containing Chlorella, which is applied to barn-fed beef cattle breeding, wherein each bull in the barn-fed beef cattle is supplied with 40 to 60 mL per day, and each cow in the barn-fed beef cattle is supplied with 70 to 100 mL per day.

[0017] In a fourth aspect, the present invention provides a method for using a nutrient solution containing Chlorella, wherein the nutrient solution is mixed with feed for housed beef cattle and then used, and the mixing ratio of the nutrient solution to the feed is 30-40 mL:5 kg.

[0018] Based on the above technical solutions, the present invention has the following beneficial effects:

[0019] (1) After the chlorella culture fluid of the present invention is subjected to high-pressure homogenization and cell disruption, the rich nutrients such as proteins, polysaccharides, and minerals in the cells are released, and in particular, the protein, which accounts for the highest weight content (about 60%) of the nutrient in chlorella, is hydrolyzed under the action of alkaline protease to generate peptides of different molecular weights. These peptides and other nutrients are directly absorbed and utilized in the gastrointestinal tract. At the same time, the small peptides in these peptides can be combined with Zn, Fe, Cu, etc. in the form of chelates and can promote the transport and absorption of these substances in cattle, so as to increase the content of trace elements in beef.

[0020] (2) The present invention hydrolyzes chlorella protein and wheat protein in wheat gluten powder simultaneously by alkaline protease and ultrasound-assisted enzymatic hydrolysis. Ultrasonic treatment makes the hydrolysis more sufficient. The chlorella protein peptides and wheat protein peptides in the hydrolyzate jointly stimulate the secretion of growth hormone in beef cattle, thereby promoting the growth and development of beef cattle, not only increasing body weight, but also optimizing muscle fibers. During the growth process of beef cattle, an appropriate amount of plant peptides helps to form more delicate muscle fibers, making the beef taste better and more tender, thereby improving the quality of beef.

[0021] (3) The present invention utilizes chlorella culture fluid and MRS liquid culture medium to co-culture Lactobacillus plantarum. The obtained Lactobacillus plantarum fermentation fluid contains not only Lactobacillus plantarum and its metabolites, but also the nutrients in chlorella. After fermentation, the typical algae odor of chlorella is greatly reduced, thereby improving the palatability of the nutrient solution. When Lactobacillus plantarum and its metabolites (lactic acid, bacteriocin, hydrogen peroxide, amylase, protease, etc.) are supplied to housed beef cattle, they can not only regulate intestinal flora and promote digestion and absorption, but also enhance immunity and prevent diseases.

[0022] (4) The nutrient solution of the present invention also contains a rumen fermentation regulator, wherein sodium bicarbonate can neutralize the excessive acidic substances produced in the rumen, thereby maintaining the acid-base balance in the rumen and improving the gastrointestinal digestion characteristics of beef cattle. Chitosan activates the complement system, mediates a series of biological effects of complement, promotes the secretion of various immune factors by the cattle, and thus enhances the body's immune system function.

[0023] In summary, the nutrient solution containing Chlorella in the present invention contains a variety of plant peptide components of different molecular weights, nutritional additives and rumen fermentation regulators, which together provide the required nutrients for the breeding of barn-fed beef cattle, and improve the immunity of barn-fed beef cattle and the quality of beef. In addition, the technical solution is not only applicable to beef cattle, but can also be used to produce nutrient solutions for other ruminants, such as nutrient solutions for mutton sheep or nutrient solutions for dairy cows, by changing the design. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the specific implementation of the present invention or the technical solution in the prior art, the drawings required for use in the specific implementation or the description of the prior art will be briefly introduced below.

[0025] Figure 1 The present invention is a process flow chart of a method for preparing a nutrient solution containing Chlorella. DETAILED DESCRIPTION

[0026] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following is combined with the attached Figure 1 The specific preparation method, ingredients and efficacy of the present invention are described in detail below. It should be noted that the chlorella in the chlorella culture solution selected in this application is Chlorella vulgaris, and the chlorella culture solution is prepared by a three-stage culture method. The specific preparation process is shown in the following description; the wheat gluten contains 70% wheat protein, and its manufacturer is Shandong Juxinlong Biotechnology Co., Ltd.; the enzyme activity of alkaline protease is 100,000 U / g, purchased from Weifang Ruichen Biotechnology Co., Ltd. Plant lactobacillus (Lactiplantibacillusplantarum) freeze-dried powder was purchased from Xi'an Zebang Biotechnology Co., Ltd., and the product specification is 10 billion cfu / g.

[0027] The three-stage culture method for preparing the Chlorella culture solution specifically comprises the following steps:

[0028] Step S1. Activate the Chlorella vulgaris and transfer it into a culture container with a volume of 1 L and containing 2 / 3 volume of BG-11. A vent is provided at the bottom of the container, and gas is introduced into the culture container through the vent. The gas flow rate is 2.5 L / min. The gas is sterilized with a cotton ball before entering the culture container. The culture is carried out for 8 days under a light intensity of 8000 Lux and a culture temperature of 27° C. to obtain a primary culture;

[0029] Step S2. The primary culture was transferred into a 50 L culture container containing 2 / 3 volume of BG-11. A vent was provided at the bottom of the container. Gas was introduced into the culture container through the vent at a flow rate of 3 L / min. The culture was carried out for 10 days at a light intensity of 8000 Lux and a culture temperature of 27° C. to obtain a secondary culture.

[0030] Step S3. The secondary culture is transferred into a culture container with a volume of 185 L and containing 2 / 3 volume of BG-11. A vent is provided at the bottom of the container, and gas is introduced into the culture container through the vent. The gas flow rate is 3 L / min. The culture is carried out for 22 days under a light intensity of 80,000 Lux and a culture temperature of 27°C to obtain a tertiary culture.

[0031] Example 1

[0032] This embodiment provides a method for preparing a nutrient solution containing Chlorella. Figure 1 , the specific technical solutions are as follows:

[0033] Step 1: Preparation of Chlorella hydrolysate

[0034] Take 100 mL of Chlorella culture solution, high-pressure homogenize for 12 min at 90 MPa to obtain Chlorella cell homogenate, add 15 g of wheat gluten, stir for 5 min to obtain a mixed solution, add 5% (5.75 g) of alkaline protease by mass of the mixed solution, adjust the pH value to 9, and perform ultrasonic-assisted enzymolysis in a 48°C water bath for 50 min to obtain an enzymolysis solution.

[0035] Step 2: Preparation of Lactobacillus plantarum fermentation broth

[0036] Preparation of Lactobacillus plantarum suspension: Open the ampoule containing the freeze-dried powder of Lactobacillus plantarum in a clean bench, mix the freeze-dried powder into a triangular flask containing 50 mL of MRS liquid culture medium, and activate and culture the mixture in a shake flask at 37° C. for 24 h to prepare a Lactobacillus plantarum suspension.

[0037] To a 250 mL Erlenmeyer flask, add 100 mL of MRS liquid culture medium and 20 mL of Chlorella culture medium, stir for 5 min, seal the flask mouth, place in an autoclave, sterilize at 0.1 MPa and 121°C for 30 min, take out from the autoclave, let stand at room temperature, inoculate 8 mL of Lactobacillus plantarum suspension, and ferment at 37°C for 10 h to obtain fermentation liquid.

[0038] Step 3: Preparation of Chlorella Products

[0039] 100 mL of Chlorella enzymatic hydrolysate and 40 mL of Lactobacillus plantarum fermentation liquid were taken and subjected to low-pressure homogenization treatment at 30 MPa for 15 min to obtain a Chlorella product.

[0040] Step 4: Prepare nutrient solution

[0041] Take 100 mL of the Chlorella product, add 8 g of a nutrient additive and 28 g of a rumen fermentation regulator, and mix well to obtain a nutrient solution containing Chlorella.

[0042] Among them, the nutritional additives include the following components in percentage by mass: 40% niacin, 2% biotin, 7% folic acid, 0.4% selenium, 13% zinc, 27% iron, 2% copper, 8% manganese, 0.3% iodine, 0.3% cobalt, and trace elements are provided by sodium selenite, zinc sulfate, ferrous sulfate, copper sulfate, manganese sulfate, sodium iodate and cobalt sulfate; the rumen fermentation regulator contains 27g sodium bicarbonate and 1g chitosan.

[0043] Example 2

[0044] This embodiment provides a method for preparing a nutrient solution containing Chlorella, and the specific technical scheme is as follows:

[0045] Step 1: Preparation of Chlorella hydrolysate

[0046] Take 100 mL of Chlorella culture solution, high-pressure homogenize for 15 min at 80 MPa to obtain Chlorella cell homogenate, add 10 g of wheat gluten, stir for 5 min to obtain a mixed solution, add 2% (2.2 g) of alkaline protease by mass of the mixed solution, adjust the pH value to 10, and perform ultrasonic-assisted enzymolysis in a 40°C water bath for 60 min to obtain an enzymolyzate.

[0047] Step 2: Preparation of Lactobacillus plantarum fermentation broth

[0048] Preparation of Lactobacillus plantarum suspension: Open the ampoule containing the freeze-dried powder of Lactobacillus plantarum in a clean bench, mix the freeze-dried powder into a triangular flask containing 50 mL of MRS liquid culture medium, and activate and culture the mixture in a shake flask at 37° C. for 24 h to prepare a Lactobacillus plantarum suspension.

[0049] To a 250 mL Erlenmeyer flask, add 100 mL of MRS liquid culture medium and 10 mL of Chlorella culture medium, stir for 5 min, seal the flask mouth, place in an autoclave, sterilize at 0.1 MPa and 121°C for 30 min, take out from the autoclave, let stand at room temperature, inoculate 8 mL of Lactobacillus plantarum suspension, and ferment at 37°C for 8 h to obtain fermentation liquid.

[0050] Step 3: Preparation of Chlorella Products

[0051] Take 100 mL of Chlorella enzymatic hydrolysate and 20 mL of Lactobacillus plantarum fermentation liquid, perform low-pressure homogenization treatment at 20 MPa for 10 min, and obtain a Chlorella product.

[0052] Step 4: Prepare nutrient solution

[0053] Take 100 mL of the Chlorella product, add 5 g of a nutrient additive and 35 g of a rumen fermentation regulator, and mix well to obtain a nutrient solution containing Chlorella.

[0054] Among them, the nutritional additives include the following components in percentage by mass: niacin 35%, biotin 3%, folic acid 5.7%, selenium 0.7%, zinc 14%, iron 32%, copper 3%, manganese 6%, iodine 0.1%, cobalt 0.5%, and trace elements are provided by sodium selenite, zinc sulfate, ferrous sulfate, copper sulfate, manganese sulfate, sodium iodate and cobalt sulfate; the rumen fermentation regulator contains 31g sodium bicarbonate and 4g chitosan.

[0055] Example 3

[0056] This embodiment provides a method for preparing a nutrient solution containing Chlorella, and the specific technical scheme is as follows:

[0057] Step 1: Preparation of Chlorella hydrolysate

[0058] Take 100 mL of Chlorella culture solution, high-pressure homogenize for 10 min under 100 MPa conditions to obtain Chlorella cell homogenate, add 20 g of wheat gluten, stir for 5 min to obtain a mixed solution, add 8% (9.6 g) of alkaline protease by mass of the mixed solution, adjust the pH value to 8, and perform ultrasonic-assisted enzymolysis in a 55°C water bath for 40 min to obtain an enzymolysis solution.

[0059] Step 2: Preparation of Lactobacillus plantarum fermentation broth

[0060] Preparation of Lactobacillus plantarum suspension: Open the ampoule containing the freeze-dried powder of Lactobacillus plantarum in a clean bench, mix the freeze-dried powder into a triangular flask containing 50 mL of MRS liquid culture medium, and activate and culture the mixture in a shake flask at 37° C. for 24 h to prepare a Lactobacillus plantarum suspension.

[0061] To a 250 mL Erlenmeyer flask, add 100 mL of MRS liquid culture medium and 30 mL of Chlorella culture medium, stir for 5 min, seal the flask mouth, place in an autoclave, sterilize at 0.1 MPa and 121°C for 30 min, take out from the autoclave, let stand at room temperature, inoculate 8 mL of Lactobacillus plantarum suspension, and ferment at 37°C for 12 h to obtain fermentation liquid.

[0062] Step 3: Preparation of Chlorella Products

[0063] Take 100 mL of Chlorella enzymatic hydrolysate and 60 mL of Lactobacillus plantarum fermentation liquid, perform low-pressure homogenization treatment at 40 MPa for 10 min, and obtain a Chlorella product.

[0064] Step 4: Prepare nutrient solution

[0065] Take 100 mL of the Chlorella product, add 10 g of a nutrient additive and 20 g of a rumen fermentation regulator, and mix well to obtain a nutrient solution containing Chlorella.

[0066] Among them, the nutritional additives include the following components in percentage by mass: 45% niacin, 1% biotin, 9% folic acid, 0.1% selenium, 11% zinc, 22% iron, 1% copper, 10% manganese, 0.5% iodine, 0.4% cobalt, and trace elements are provided by sodium selenite, zinc sulfate, ferrous sulfate, copper sulfate, manganese sulfate, sodium iodate and cobalt sulfate; the rumen fermentation regulator contains 18g sodium bicarbonate and 2g chitosan.

[0067] Example 4

[0068] This embodiment provides a method for preparing a nutrient solution containing Chlorella, and the specific technical scheme is as follows:

[0069] Step 1: Preparation of Chlorella hydrolysate

[0070] Take 100 mL of Chlorella culture solution, high-pressure homogenize for 10 min at 95 MPa to obtain Chlorella cell homogenate, add 20 g of wheat gluten, stir for 5 min to obtain a mixed solution, add 8% (9.6 g) of alkaline protease by mass of the mixed solution, adjust the pH value to 8, and perform ultrasonic-assisted enzymolysis in a 50°C water bath for 40 min to obtain an enzymolysis solution.

[0071] Step 2: Preparation of Lactobacillus plantarum fermentation broth

[0072] Preparation of Lactobacillus plantarum suspension: Open the ampoule containing the freeze-dried powder of Lactobacillus plantarum in a clean bench, mix the freeze-dried powder into a triangular flask containing 50 mL of MRS liquid culture medium, and activate and culture the mixture in a shake flask at 37° C. for 24 h to prepare a Lactobacillus plantarum suspension.

[0073] To a 250 mL Erlenmeyer flask, add 100 mL of MRS liquid culture medium and 30 mL of Chlorella culture medium, stir for 5 min, seal the flask mouth, place in an autoclave, sterilize at 0.1 MPa and 121°C for 30 min, take out from the autoclave, let stand at room temperature, inoculate 8 mL of Lactobacillus plantarum suspension, and ferment at 37°C for 12 h to obtain fermentation liquid.

[0074] Step 3: Preparation of Chlorella Products

[0075] 100 mL of Chlorella enzymatic hydrolysate and 60 mL of Lactobacillus plantarum fermentation liquid were taken and subjected to low-pressure homogenization treatment at 35 MPa for 10 min to obtain a Chlorella product.

[0076] Step 4: Prepare nutrient solution

[0077] Take 100 mL of the Chlorella product, add 10 g of a nutrient additive and 20 g of a rumen fermentation regulator, and mix well to obtain a nutrient solution containing Chlorella.

[0078] Among them, the nutritional additives include the following components in percentage by mass: 40% niacin, 2% biotin, 5% folic acid, 0.4% selenium, 13% zinc, 27% iron, 2% copper, 10% manganese, 0.5% iodine, 0.1% cobalt, and trace elements are provided by sodium selenite, zinc sulfate, ferrous sulfate, copper sulfate, manganese sulfate, sodium iodate and cobalt sulfate; the rumen fermentation regulator contains 18g sodium bicarbonate and 2g chitosan.

[0079] It should be noted that in Examples 1-4, the order of step 1 and step 2 can be interchanged without affecting the preparation and use of the nutrient solution.

[0080] Comparative Example 1

[0081] The difference between this comparative example and Example 1 is that, in step 1, when preparing the Chlorella hydrolysate, no wheat gluten is added.

[0082] Comparative Example 2

[0083] The difference between this comparative example and Example 1 is that, in step 2, when preparing the Lactobacillus plantarum fermentation broth, no Chlorella culture broth is added to the MRS culture medium.

[0084] Comparative Example 3

[0085] The difference between this comparative example and Example 1 is that, when the nutrient solution is prepared in step 4, the rumen fermentation regulator does not contain sodium bicarbonate.

[0086] Comparative Example 4

[0087] The difference between this comparative example and Example 1 is that, when the nutrient solution is prepared in step 4, the rumen fermentation regulator does not contain chitosan.

[0088] Performance Testing

[0089] In order to explore the effect of the nutrient solution containing Chlorella prepared by the present invention on the immune function and meat quality of housed beef cattle, 45 housed Horqin bulls weighing 380 to 400 kg were selected as animal models, and they were randomly divided into 9 groups, i.e., a control group and 8 experimental groups, with 5 bulls in each group.

[0090] The blank control group was fed with a basic diet, and the eight test groups were fed with 50 mL / head / day of nutrient solution in the basic diet at a ratio of 7 mL: 1 kg (the nutrient solutions in the eight test groups were the nutrient solutions prepared by the methods in Examples 1-4 and Comparative Examples 1-4, respectively). A 70-day feeding comparison test was conducted, wherein the 1st to 10th days were the pre-test period, and the 11th to 70th days were the test period. During the pre-test period, numbering, deworming, and stomachic were performed, and the deworming drug was ivermectin, and rhubarb baking soda was used to strengthen the stomach after deworming. At the beginning (11th day) and the end (70th day) of the test period, blood was collected from the jugular vein of each group of test cattle and serum was separated for detecting the content of immune indicators lysozyme (LZM), nitric oxide (NO), acid phosphatase (ACP) and immunoglobulin G (IgG). The test results of immune indicators in the serum of each group of cattle (the average value of the test results of 5 cattle in each group) are shown in Table 1.

[0091] Table 1 Detection of immune indexes in bovine serum

[0092]

[0093]

[0094] According to the results of immune index detection in bovine serum in Table 1, compared with Comparative Examples 1-4 and the blank control example, the immune indexes of lysozyme, nitric oxide, acid phosphatase and immunoglobulin G in the bovine serum of the test group using the nutrient solution prepared in Examples 1-4 were greatly improved, indicating that the nutrient solution provided in the embodiment of the present invention can significantly improve the immune performance of house-fed beef cattle. At the same time, the bovine serum immune index in the blank control group fed only with the basic diet was the lowest, indicating that the basic diet alone could not meet the immune needs of house-fed beef cattle, and the immunity of the cattle body was low.

[0095] After the experiment, each group randomly slaughtered one cow and took 30g of the longissimus dorsi muscle sample for fat content detection and analysis. The fat detection process is as follows: mince 30g of the sample, place the minced sample in a 250mL conical flask, add 100mL of 2mol / L hydrochloric acid solution, heat and boil to release the fat in the meat, filter to obtain the filter residue containing fat, place the filter residue and filter paper in an oven to dry to remove moisture, put the dried filter paper and filter residue into a Soxhlet extractor, add 100mL of petroleum ether as an extractant, extract the fat in the filter residue by Soxhlet extraction, evaporate the extractant after extraction, obtain pure fat, and weigh to obtain the fat content in the longissimus dorsi muscle sample. The results of the fat content detection of the longissimus dorsi muscle of cattle are shown in Table 2.

[0096] Table 2 Results of fat content test on longissimus dorsi muscle of cattle

[0097]

[0098]

[0099] As shown in Table 2, the fat content of the longissimus dorsi muscle of cattle in Examples 1-4 is significantly lower than that in Comparative Examples 1-4 and the blank control, indicating that the nutrient solution provided by the present invention can reduce fat deposition in housed beef cattle, improve beef quality, and thus avoid diseases such as fatty liver caused by excessive fat deposition.

[0100] In addition to the above performance tests using barn-fed Korchin bulls as animal models, the inventors also conducted performance tests using barn-fed Korchin cows as animal models and found that the nutrient solution prepared by the present invention is also suitable for cows and can also improve the immune performance of cows and the quality of beef.

[0101] In addition, based on the nutrient solution containing Chlorella provided by the present invention, nutrient solutions for other ruminants can be prepared after further formula optimization.

[0102] In summary, the nutrient solution containing Chlorella provided by the present invention contains a variety of plant peptide components with different molecular weights, nutritional additives and rumen fermentation regulators, which together provide the required nutrients for the breeding of housed beef cattle, reduce fat deposition, and improve the immunity of housed beef cattle and the quality of beef.

[0103] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A nutrient solution containing Chlorella, characterized in that: The nutrient solution comprises a chlorella product, a nutrient additive and a rumen fermentation regulator, wherein the ratio of the chlorella product, the nutrient additive and the rumen fermentation regulator is 100 mL: (5-10) g: (20-35) g; the chlorella product is prepared from chlorella enzymatic hydrolysate and Lactobacillus plantarum fermentation liquid; the nutrient additive comprises vitamins and trace elements; and the rumen fermentation regulator comprises sodium bicarbonate and chitosan.

2. The nutrient solution containing Chlorella according to claim 1, characterized in that: The chlorella product is obtained by mixing chlorella hydrolysate and plant lactobacillus fermentation liquid in a volume ratio of 5:(1-3) and subjecting the mixture to low-pressure homogenization for 10-15 minutes, wherein the low-pressure homogenization is carried out at 20-40 MPa.

3. A nutrient solution containing Chlorella according to claim 1 or 2, characterized in that: The preparation process of the chlorella hydrolysate is as follows: 100 mL of chlorella culture solution is taken, and high-pressure homogenization is performed for 10 minutes under the condition of 80-100 MPa to obtain a chlorella cell homogenate, 10-20 g of wheat gluten is added, and the mixture is stirred for 5 minutes to obtain a mixed solution, and alkaline protease accounting for 2-8% of the mass of the mixed solution is added, and the pH value is adjusted to 8-10, and ultrasonic-assisted enzymolysis is performed in a water bath at 40-55° C. for 40-60 minutes to obtain an enzymolysate.

4. A nutrient solution containing Chlorella according to claim 1 or 2, characterized in that: The plant lactobacillus fermentation liquid is prepared according to the following steps: adding chlorella culture liquid to MRS liquid culture medium and sterilizing at high temperature, inoculating plant lactobacillus suspension after standing at room temperature, and fermenting at 37°C for 8-12 hours to obtain fermentation liquid, wherein the volume ratio of the chlorella culture liquid, the MRS liquid culture medium and the plant lactobacillus suspension is (0.1-0.3):1:0.

08.

5. The nutrient solution containing Chlorella according to claim 1, characterized in that: The vitamins include niacin, biotin and folic acid, and the trace elements include selenium, zinc, iron, copper, manganese, iodine and cobalt.

6. The nutrient solution containing Chlorella according to claim 5, characterized in that: Taking the total mass of the nutritional additive as 100%, the nutritional additive includes the following components in the following mass percentages: 35-45% niacin, 1-3% biotin, 5-9% folic acid, 0.1-0.7% selenium, 11-14% zinc, 22-32% iron, 1-3% copper, 6-10% manganese, 0.1-0.5% iodine, and 0.1-0.5% cobalt.

7. The nutrient solution containing Chlorella according to claim 1, characterized in that: The mass ratio of sodium bicarbonate to chitosan in the rumen fermentation regulator is 100:(1-10).

8. The method for preparing a nutrient solution containing Chlorella according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: adding a nutrient additive and a rumen fermentation regulator into the chlorella product in proportion and mixing the mixture to obtain a nutrient solution containing chlorella.

9. The use of a nutrient solution containing Chlorella according to any one of claims 1 to 7, characterized in that: It is used in stall-fed beef cattle breeding, wherein each bull among the stall-fed beef cattle is supplied with 40-60 mL per day, and each cow among the stall-fed beef cattle is supplied with 70-100 mL per day.

10. The method for using the nutrient solution containing Chlorella according to any one of claims 1 to 7, characterized in that: The nutrient solution is mixed with feed for housed beef cattle and then used, and the mixing ratio of the nutrient solution to the feed is 30-40 mL: 5 kg.

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