Light supplementing and seedling raising method for improving seedling strengthening effect of asparagus lettuce

Abstract

The invention belongs to the technical field of crop planting, and particularly discloses a light supplementing and seedling raising method for improving the seedling strengthening effect of asparagus lettuce. The light supplementing method for improving the seedling strengthening effect of the asparagus lettuce comprises the steps that in the seedling raising stage, the light intensity of white light is set to be 120 mu mol / m < 2 > / s, the light period is set to be 14 h / 10 h, the natural weak light environment is simulated, red light and blue light of 100 mu mol / m < 2 > / s are supplemented, and the ratio of the red light to the blue light is 7: 3. According to the method, red light and blue light (the ratio of red light to blue light is 7: 3) are supplemented under the weak light background (the light intensity of white light is 120 mu mol / m < 2 > / s), so that adverse effects caused by weak light can be eliminated, the seedling strengthening effect of the asparagus lettuce is improved, and the biomass and the leaf number of the asparagus lettuce seedlings can be increased. The dry weights of the whole plants of the Du-qing bamboo shoot and the red tip bamboo shoot No.1 are respectively increased by 63.7% and 114.8% compared with those of the Du-qing bamboo shoot and the red tip bamboo shoot No.1 under the weak light condition under the treatment of the light supplementing and seedling raising methods.

Description

Technical Field

[0001] The invention relates to the technical field of crop planting, and in particular to a light supplement and seedling raising method for improving the effect of strengthening lettuce seedlings. Background Art

[0002] Lettuce, also known as lettuce, has edible above-ground stems. It is a common vegetable in people's daily lives and is widely planted throughout my country. Lettuce is generally planted by seedling transplanting, and the demand for seedlings in production is relatively large. During the seedling raising process, the light in the seedling raising environment is often weak due to factors such as weather, which affects the seedling raising effect and seedling raising cycle. For example, spring lettuce is generally raised in greenhouses from December to March of the following year. The light conditions in winter are generally weak, and the barrier of light caused by the covering materials of the facilities often leads to insufficient light during the seedling raising period.

[0003] Therefore, the present invention explores the phenomenon that lettuce seedlings grow poorly in a weak light environment, performs light supplementation under simulated weak light environment conditions, and explores a light supplementation scheme for lettuce seedlings in low-light areas and under poor light conditions, providing a reference for ensuring the quality of lettuce seedlings. Summary of the invention

[0004] The invention aims to improve the effect of raising lettuce seedlings under low light conditions, increase the seedling index and growth rate of lettuce seedlings under low light conditions, and provide a light supplement method for lettuce under low light conditions.

[0005] In order to achieve the above purpose, the first technical solution adopted by the present invention is: A light supplement method for improving the growth effect of lettuce seedlings: in the seedling stage, the white light intensity is set to 120umol / m 2 / s, the photoperiod was 14h / 10h to simulate the natural weak light environment, and 100umol / m 2 / s of red and blue light, the light cycle is 14h / 10h, and the ratio of red light to blue light is 7:3.

[0006] The second technical solution adopted by the present invention is: A seedling raising method for improving the effect of strengthening lettuce seedlings, adopting the light supplementing method for improving the effect of strengthening lettuce seedlings of the first technical solution to raise seedlings, specifically comprising: The lettuce seeds are germinated, and the seeds are sown after 1-2 days of germination when they turn white; The white seeds are sown and raised as seedlings. The ambient temperature during the seedling raising stage is 23-25°C, the relative humidity is 60%-70%, and the light environment is set according to the light supplementation method for improving the effect of strengthening lettuce seedlings as described in the first technical solution.

[0007] Preferably, the germination method is: placing the lettuce seeds in a culture dish covered with filter paper, and then covering the culture dish with the filter paper, keeping the culture dish moist and free of water, and the ambient temperature is 23-25°C.

[0008] Preferably, the seedling substrate used in the seedling raising process is obtained by mixing peat, perlite and vermiculite in a mass ratio of 9:3:1 and sterilizing the mixture.

[0009] Preferably, sterilization is carried out at 121°C for 15-20 minutes.

[0010] Preferably, during the seedling raising stage, tidal irrigation is performed every two days, with clean water used for irrigation before true leaves grow out, and nutrient solution used for irrigation after true leaves begin to grow out.

[0011] Preferably, the pH value of the nutrient solution is 6.5-7.5, and the EC value is 1200-1300 μS / cm.

[0012] Compared with the prior art, the present invention has the following beneficial effects: The present invention is effective in weak light background (white light intensity 120umol / m 2 / s) and supplementing red and blue light (the ratio of red light to blue light is 7:3) can eliminate the adverse effects of weak light, improve the effect of lettuce seedlings, and increase the biomass and leaf number of lettuce seedlings. Compared with the weak light condition, the whole plant dry weight of Zhongduqing and Hongjiansun No. 1 under the light supplement and seedling raising method of the present invention increased by 63.7% and 114.8%, respectively. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is the seedling index of Zhongduqing under different treatments; Figure 2 This is the seedling index of Hongjiansun No. 1 under different treatments; DETAILED DESCRIPTION

[0014] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments.It should be understood that these descriptions are exemplary only, and are not intended to limit the scope of the present invention.In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.Unspecified conditions in the embodiments are carried out according to the conditions of normal conditions or manufacturer recommendations.Reagents used or instruments that do not specify manufacturers are conventional products that can be purchased and obtained commercially.

[0015] The present invention determines the weak white light background according to the average actual light intensity of seedlings in plastic greenhouses in Chengdu in winter, and then performs a supplementary light experiment under this background light intensity. This supplementary light scheme takes the natural environment light into consideration, and then supplements red and blue light, which can reduce the input of supplementary light energy during seedling cultivation under insufficient light conditions to a certain extent. Moreover, this supplementary light method can significantly improve the seedling index of lettuce seedlings, and has a significant promoting effect on the number of seedling leaves in the same period of time. The supplementary light and seedling cultivation method of the present invention can also significantly improve the underground growth of lettuce seedlings, and has certain potential in shortening the seedling acclimatization time and growth state after seedling transplanting.

[0016] The first embodiment of the present invention provides a light supplement method for improving the effect of growing lettuce seedlings. In the seedling stage, the white light intensity is set to 120umol / m 2 / s, the photoperiod was 14h / 10h to simulate the natural weak light environment, and 100umol / m 2 / s of red and blue light, the light cycle is 14h / 10h, and the ratio of red light to blue light is 7:3.

[0017] Plants mainly absorb red and blue light spectrum for photosynthesis, but other wavelengths of light also have certain positive effects on plant growth. Therefore, supplementing red and blue light in a full-spectrum weak light environment has a positive significance for plant growth. The present invention unexpectedly found that supplementing red and blue light with a ratio of 7:3 under a weak light background has a significant improvement in the effect of strengthening lettuce seedlings.

[0018] The second embodiment of the present invention provides a seedling raising method for improving the effect of strengthening lettuce seedlings, which adopts the light supplementation method for improving the effect of strengthening lettuce seedlings of the first embodiment to raise seedlings, and specifically comprises: The lettuce seeds are germinated, and the seeds are sown after 1-2 days of germination when they turn white; The white seeds are sown and raised as seedlings. The ambient temperature during the seedling raising stage is 23-25°C, the relative humidity is 60%-70%, and the light environment is set according to the light supplementation method for improving the effect of strengthening lettuce seedlings as described in the first technical solution.

[0019] The germination method, seedling tray, seedling medium, nutrient solution, irrigation method, etc. in the lettuce seedling raising process are all commonly used means in the field and can be selected by those skilled in the art according to actual conditions.

[0020] In order to make the technical solution of the present invention clearer, the lighting supplement and seedling raising method and the effect of improving the lettuce seedling strengthening effect are described in detail through a plurality of specific embodiments below.

[0021] Example 1 Step 1 (setting the light environment for seedling cultivation): The temperature of the seedling cultivation environment is controlled in the range of 23-25℃, and the humidity is maintained in the range of 60%-70%. Use LED white light to simulate the natural weak light environment, and set the light cycle to 14h / 10h. Refer to the light intensity inside the plastic greenhouse and set the white light intensity to 120umol / m 2 / s. Then supplement 100umol / m 2 / s of red and blue light, and the light cycle is 14h / 10h. The ratio of red light to blue light is set in multiple experimental groups and control groups, as shown in Table 1. R represents red light, and B represents blue light. For example, "7R:3B" means that the light quality ratio of red and blue light is 7:3; "W120" represents the intensity of white light, that is, white light 120umol / m 2 / s; "W120+R" represents the white light intensity of 120umol / m 2 / s combined with 100umol / m 2 / s of pure red light.

[0022] Step 2 (germination): Place the seeds in a culture dish covered with filter paper and cover it with filter paper. Then wet the filter paper with clean water to ensure that the moisture content inside the culture dish is constant. It is best to place the culture dish at room temperature of 23-25℃ so that there is no water accumulation in the culture dish. Sow the seeds after 1-2 days when they turn white. During this period, keep the water content inside the culture dish sufficient to avoid insufficient humidity.

[0023] Step 3 (preparation of seedling medium): Select a clean, non-toxic hole tray with 105 holes for seedling cultivation. The ratio of seedling medium is peat: perlite: vermiculite = 9:3:1. Sterilize the mixed medium at 121℃ for 15-20 minutes. Add water to the sterilized medium and stir evenly. The amount of water added should be enough for the medium to hold without falling apart. Then put the medium into the seedling tray for use.

[0024] Step 4 (Sowing): Select white seeds for sowing, place one seed in each hole, and then cover the surface with a thin layer of substrate. After sowing, place the trays under the corresponding fill light for cultivation, and perform tidal irrigation to ensure that the substrate is fully soaked.

[0025] Step 5 (seedling management): Tidal irrigation is carried out every two days. Use clean water for irrigation before the true leaves grow out, and use nutrient solution for irrigation after the true leaves begin to grow out. The pH value of the nutrient solution is 6.5-7.5, and the EC value is controlled at 1200-1300μS / cm. The nutrient solution is filtered and returned to the storage tank for recycling.

[0026] The above seedling raising method was applied to the seeds of Zhongduqing and Hongjiansun No. 1, and the measurements were recorded 20 days after sowing. The plant height, stem diameter, whole plant dry weight and number of leaves of lettuce obtained from each experimental group and control group of Zhongduqing and Hongjiansun No. 1 were measured by the above method to compare the growth differences of lettuce under different treatments. The results are shown in Table 1-2. The seedling index of Zhongduqing and Hongjiansun No. 1 was calculated, respectively, and the seedling index = (stem diameter / plant height + underground dry weight / above ground dry weight) * total dry weight. The results are as follows Figure 1 , 2 shown.

[0027] Table 1 Growth differences of different species of Duqing under different supplementary light treatments .

[0028] Table 2 Growth differences of Hongjiansun 1 under different supplementary lighting treatments .

[0029] From Table 1-2, we can see that the lettuce showed good seedling growth and dry matter accumulation when supplemented with 7R:3B, and had a significant effect on improving the leggy growth of seedlings. The stem thickness, whole plant dry weight and leaf number were significantly improved, and various morphological indicators were significantly better than those in weak light conditions. In the same period of time, the number of lettuce leaves under supplementary light conditions was significantly better than that under weak light conditions, which significantly improved the leggy growth of seedlings under weak light.

[0030] pass Figure 1-2 It can be seen that compared with the low-light environment, the 7R:3B supplementary lighting solution can significantly improve the seedling index, and it also performs best compared with other supplementary lighting solutions.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A light supplementation method for improving the effect of lettuce seedling growth, characterized in that: During the seedling stage, the white light intensity was set to 120umol / m 2 / s, the photoperiod was 14h / 10h to simulate the natural weak light environment, and 100umol / m 2 / s of red and blue light, the light cycle is 14h / 10h, and the ratio of red light to blue light is 7:

3.

2. A method for raising seedlings for improving the effect of growing lettuce seedlings, characterized in that: The method for supplementing light for improving the effect of strengthening lettuce seedlings as claimed in claim 1 is used for seedling cultivation, specifically comprising: The lettuce seeds are germinated, and the seeds are sown after 1-2 days of germination when they turn white; The whitened seeds are sown and raised for seedling raising. The ambient temperature during the seedling raising stage is 23-25° C., the relative humidity is 60%-70%, and the light environment setting adopts the light supplementation method for improving the effect of strengthening lettuce seedlings as described in claim 1.

3. The seedling raising method according to claim 2, characterized in that: The germination method is: place the lettuce seeds in a culture dish covered with filter paper, then cover it with the filter paper, keep the culture dish moist and without water accumulation, and the ambient temperature is 23-25℃.

4. The method for raising seedlings according to claim 2, wherein: The seedling medium used in the seedling raising process is obtained by mixing peat, perlite and vermiculite in a mass ratio of 9:3:1 and then sterilizing the mixture.

5. The method according to claim 4, characterized in that Sterilize at 121°C for 15-20 minutes.

6. The method according to claim 2, characterized in that During the seedling stage, tidal irrigation is carried out every two days. Clean water is used for irrigation before true leaves grow, and nutrient solution is used for irrigation after true leaves begin to grow.

7. The method according to claim 6, characterized in that The pH value of the nutrient solution is 6.5-7.5, and the EC value is 1200-1300 μS / cm.

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