Carbon dioxide incubator for bioengineering

Abstract

The invention belongs to the technical field of bioengineering related equipment, and particularly discloses a bioengineering carbon dioxide incubator which comprises an incubator body, a mounting cavity is formed in the surface of the incubator body, and an incubator door used for shielding the mounting cavity is arranged on the surface of the incubator body; by arranging the gas injection module, carbon dioxide guided into the containing cavity can be fully mixed, it is guaranteed that the concentration of the carbon dioxide in the injected mixed gas is balanced, meanwhile, gas flowing in the box body is enhanced, and it is guaranteed that the concentration of the carbon dioxide in the containing cavity always meets the culture conditions of biological tissue; a good environment is provided for culture of biological tissues, and a second detector, a guide shell, an exhaust pipe and a branch pipe are utilized, so that the carbon dioxide concentration of gas entering the guide shell can be further detected through the second detector; the gas meeting the concentration requirement can be guided back into the containing box to be reused, gas flowing in the containing box is enhanced, and the situation of carbon dioxide deposition is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of bioengineering related equipment, and specifically discloses a bioengineering carbon dioxide incubator. Background Art

[0002] A carbon dioxide incubator is a device for culturing cells / tissues in vitro by simulating a growth environment similar to that of cells / tissues in a living body. The incubator requires a stable temperature (37°C), a stable CO2 level (5%), a constant pH (pH: 7.2-7.4), and a high relative saturation humidity (95%). It is an advanced instrument for culturing cells, tissues, and bacteria, and is a key device necessary for immunology, oncology, genetics, and bioengineering. When the carbon dioxide concentration inside the existing carbon dioxide incubator is lower than the preset concentration, carbon dioxide is usually directly injected to increase the concentration. However, the directly injected carbon dioxide is unevenly distributed inside the incubator, which easily causes uneven carbon dioxide concentrations in various places and affects the culture effect. Therefore, a person skilled in the art has proposed a carbon dioxide incubator for bioengineering. Summary of the invention

[0003] In view of this, the technical problem to be solved by the present invention is to provide a bioengineering carbon dioxide incubator to solve the problem of uneven distribution of carbon dioxide refilled inside the box in the prior art.

[0004] To achieve the above object, the present invention provides a bioengineering carbon dioxide incubator, comprising a box body, a mounting cavity is provided on the surface of the box body, a box door for shielding the mounting cavity is provided on the surface of the box body, an inner core module is provided inside the mounting cavity, and a gas injection module is provided on one side of the box body; Among them, the gas injection module includes an air pump arranged on one side of the box body, the air inlet end of the air pump is connected to a degassing pipe, the surface of the degassing pipe is connected to evenly distributed connecting pipes, the surface of the connecting pipe is connected to an exhaust pipe for exhausting air inside the installation cavity, the surface of the exhaust pipe is connected to a branch pipe connected to the inside of the box body, solenoid valves are arranged inside the exhaust pipe and the branch pipe, a connecting box is arranged on one side of the air pump, a filter is arranged inside the connecting box, the air outlet end of the air pump is connected to the connecting box, and a mixing assembly is arranged on one side of the connecting box.

[0005] In the above technical solution, preferably, the gas injection module also includes several groups of guide shells arranged inside the box body, one end of the guide shell is connected to the exhaust pipe, a second detector is arranged on the top of the guide shell, the detection end of the second detector extends to the interior of the guide shell, and a side of the top of the guide shell away from the exhaust pipe is provided with evenly distributed air inlet holes.

[0006] In the above technical solution, preferably, the mixing assembly includes a mixing tank connected to one side of the connecting box, the inner wall of the mixing tank is fixedly connected with a connecting plate, the surface of the connecting plate is provided with evenly distributed guide grooves, and the setting of the guide grooves can make the gas passing through the guide grooves rise in a spiral shape.

[0007] In the above technical solution, preferably, the inner wall of the mixing tank is fixedly connected with a mounting shell located above the connecting plate, the top of the mixing tank is provided with an inlet pipe for introducing carbon dioxide, the lower end of the inlet pipe passes through the mounting shell and is connected with a second nozzle evenly distributed, the inner wall of the mounting shell is fixedly connected with three connecting plates connected end to end, and a detection cavity is formed between the top connecting plate and the inner wall of the mounting shell, the top of the mixing tank is provided with a first detector, the detection probe of the first detector extends into the interior of the detection cavity, and the top of the mixing tank is provided with a discharge pipe connected to the detection cavity.

[0008] In the above technical solution, preferably, evenly distributed dividing strips are fixedly connected to the inner side of the connecting disk, and the dividing strips arranged on the middle connecting disk are staggered with the dividing strips arranged on the other two connecting disks.

[0009] In the above technical solution, preferably, evenly distributed installation pipes are provided on one side of the box body, one end of the discharge pipe is connected to the adjacent installation pipe, the surface of the installation pipe is connected to evenly distributed injection pipes, and the other end surface of the injection pipe is connected to two first nozzles.

[0010] In the above technical solution, preferably, the inner core module includes three horizontal plates fixedly connected to the inner wall of the installation cavity and symmetrically distributed, the top of the horizontal plate is fixedly connected with evenly distributed vertical plates, a mounting block is fixedly connected between two adjacent vertical plates, a placement cavity is formed between the mounting block and the horizontal plate, and a placement box is arranged inside the placement cavity.

[0011] In the above technical solution, preferably, a block for shielding the placement cavity is fixedly connected to one side of the placement box, a baffle is provided at the bottom of the mounting block, two symmetrically distributed rotating shafts are fixedly connected to the surface of the baffle, the rotating shafts are rotatably connected to the inner walls of the adjacent vertical plates, a spring is fixedly connected to the surface of the rotating shaft, and the other end of the spring is fixedly connected to the inner wall of the vertical plate.

[0012] In the above technical solution, preferably, the injection pipe extends to the interior of the mounting block, and the lower end of the first nozzle passes through the mounting block.

[0013] In the above technical solution, preferably, grooves matching the guide shell are opened on both sides of the placement box, connecting holes connected to the placement cavity are opened on the inner wall of the placement box, and discharge holes evenly distributed and connected to the grooves are opened on the inner wall of the placement box.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. By setting up a gas injection module, the carbon dioxide introduced into the placement chamber can be fully mixed to ensure the balance of the carbon dioxide concentration in the injected mixed gas. At the same time, the gas flow inside the box is strengthened to ensure that the carbon dioxide concentration inside the placement chamber always meets the culture conditions of biological tissues, providing a good environment for the culture of biological tissues. In addition, by using a second detector, a guide shell, an exhaust pipe and a branch pipe, the carbon dioxide concentration of the gas entering the guide shell can be further detected by the second detector. The gas that meets the concentration requirements can be guided back to the placement box for reuse and the gas flow inside the placement box can be strengthened to avoid carbon dioxide deposition.

[0015] 2. By setting connecting plates, guide grooves, dividing strips and connecting plates, the gas introduced into the mixing chamber forms a spiral upward airflow through the guide groove to enhance the mixing effect with carbon dioxide. Under the action of the dividing strips and connecting plates, the mixing effect of the two can be further enhanced, so that the carbon dioxide is evenly distributed, the detection accuracy is improved and the error is reduced.

[0016] 3. By setting up the inner core module, when taking out a placement box separately, the carbon dioxide concentration inside other placement chambers will not be affected, avoiding the situation that the overall carbon dioxide concentration will be destroyed after the traditional box door is opened. The subsequent readjustment operation is more cumbersome, and after the placement box is pulled out, the spring drives the shaft to rotate so that the baffle covers the placement chamber, reducing the outside air from entering the placement chamber, and maximally reducing the difficulty of carbon dioxide concentration regulation. The mixed gas can be injected into the placement chamber through the injection tube and the first nozzle for biological tissue culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of the present invention; Figure 2 It is a schematic diagram of extracting the placement box of the present invention; Figure 3 It is a structural schematic diagram of the inner core module of the present invention; Figure 4 This is a schematic diagram of the connection between the injection pipe, the mounting pipe and the mounting block of the present invention; Figure 5 It is a structural schematic diagram of the gas injection module of the present invention; Figure 6 Schematic diagram of the distribution of the guide grooves of the present invention; Figure 7 This is a schematic diagram of the connection between the connecting plate and the separator strip of the present invention; Figure 8 It is a schematic diagram of the distribution of the connection holes, the discharge holes and the grooves of the present invention; Fig. 9 It is a schematic diagram of the connection between the exhaust pipe and the branch pipe of the present invention.

[0018] In the figure: 1, box body; 101, box door; 2, gas injection module; 201, exhaust pipe; 202, connecting pipe; 203, installation pipe; 204, injection pipe; 205, first nozzle; 206, outlet pipe; 207, air pump; 208, connecting box; 209, filter; 210, mixing tank; 211, inlet pipe; 212, outlet pipe; 213, second nozzle; 214, first detector; 215, connecting plate; 2 16. Guide groove; 217. Mounting shell; 218. Connecting plate; 219. Dividing strip; 220. Branch pipe; 221. Guide shell; 222. Second detector; 223. Air inlet; 3. Inner core module; 301. Vertical plate; 302. Horizontal plate; 303. Placement box; 304. Block; 305. Mounting block; 306. Baffle; 307. Spring; 308. Groove; 309. Connecting hole; 310. Discharge hole. DETAILED DESCRIPTION

[0019] In order to more clearly understand the above-mentioned objects, features and advantages of the present invention, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0020] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0021] like Figure 1-Figure 9 A bioengineering carbon dioxide incubator shown includes a box body 1, a mounting cavity is provided on the surface of the box body 1, a box door 101 for shielding the mounting cavity is provided on the surface of the box body 1, an inner core module 3 is provided inside the mounting cavity, and a gas injection module 2 is provided on one side of the box body 1; Specifically, a pressure relief valve is arranged inside the box 1 to release pressure when the pressure is too high to reduce damage to the box 1. This technical means is a relatively mature component in the application of existing technical means, so it is not shown in the figure.

[0022] Among them, the gas injection module 2 includes an air pump 207 arranged on one side of the box body 1, the air inlet end of the air pump 207 is connected to the outlet pipe 206, the surface of the outlet pipe 206 is connected to the evenly distributed connecting pipes 202, the surface of the connecting pipe 202 is connected to the exhaust pipe 201 for exhausting air inside the installation cavity, the surface of the exhaust pipe 201 is connected to the branch pipe 220 connected to the inside of the box body 1, and the exhaust pipe 201 and the branch pipe 220 are both provided with solenoid valves, a connecting box 208 is provided on one side of the air pump 207, a filter screen 209 is provided inside the connecting box 208, the air outlet end of the air pump 207 is connected to the connecting box 208, and a mixing component is provided on one side of the connecting box 208.

[0023] like Figure 1-Figure 9 As shown, the gas injection module 2 also includes a plurality of guide shells 221 arranged inside the box body 1, one end of the guide shell 221 is connected to the exhaust pipe 201, a second detector 222 is arranged on the top of the guide shell 221, and the detection end of the second detector 222 extends to the interior of the guide shell 221, and a side of the top of the guide shell 221 away from the exhaust pipe 201 is provided with evenly distributed air inlet holes 223.

[0024] The mixing assembly includes a mixing tank 210 connected to one side of the connecting box 208. The inner wall of the mixing tank 210 is fixedly connected with a connecting plate 215. The surface of the connecting plate 215 is provided with evenly distributed guide grooves 216. The setting of the guide grooves 216 can enable the gas passing through the guide grooves 216 to rise in a spiral shape.

[0025] The inner wall of the mixing tank 210 is fixedly connected to a mounting shell 217 located above the connecting plate 215, and the top of the mixing tank 210 is provided with an inlet pipe 211 for introducing carbon dioxide, and the lower end of the inlet pipe 211 passes through the mounting shell 217 and is connected to a uniformly distributed second nozzle 213, and the inner wall of the mounting shell 217 is fixedly connected to three connecting plates 218 connected end to end, and a detection cavity is formed between the top connecting plate 218 and the inner wall of the mounting shell 217, and the top of the mixing tank 210 is provided with a first detector 214, and the detection probe of the first detector 214 extends to the inside of the detection cavity, and the top of the mixing tank 210 is provided with an exhaust pipe 212 connected to the detection cavity.

[0026] The inner side of the connection disk 218 is fixedly connected with evenly distributed dividing strips 219 , and the dividing strips 219 arranged on the middle connection disk 218 are staggered with the dividing strips 219 arranged on the other two connection disks 218 .

[0027] One side of the box body 1 is provided with evenly distributed installation pipes 203, one end of the discharge pipe 212 is connected to the adjacent installation pipe 203, the surface of the installation pipe 203 is connected to evenly distributed injection pipes 204, and the other end surface of the injection pipe 204 is connected to two first nozzles 205.

[0028] The first detector 214 and the second detector 222 are both carbon dioxide concentration detectors, capable of detecting the concentration of carbon dioxide; The gas entering the guide shell 221 through the air inlet 223 can be tested for concentration under the action of the second detector 222. If the concentration is lower than the preset value, the solenoid valve inside the branch pipe 220 is closed, and the solenoid valve inside the exhaust pipe 201 is opened, so that the gas can be discharged through the exhaust pipe 201. When the concentration meets the preset value requirement, the solenoid valve inside the branch pipe 220 is opened, and the solenoid valve inside the exhaust pipe 201 is closed, so that the gas can be guided back to the inside of the box 1 through the branch pipe 220. When the second detector 222 detects that the concentration is lower than the preset value, the air pump 207 is started to extract the air inside the installation cavity under the action of the outlet pipe 206, the connecting pipe 202 and the exhaust pipe 201, and the air is introduced into the interior of the connecting box 208, filtered by the filter 209, and then introduced into the interior of the mixing tank 210. The gas injected into the mixing tank 210 passes through the guide groove 216 and passes through the connecting plate 215. In this process, the gas rises in a spiral shape. At this time, carbon dioxide is injected into the interior of the mixing tank 210 through the inlet pipe 211 and discharged through the second nozzle 213. The spiral airflow formed at this time can enhance the mixing effect with carbon dioxide. The mixed gas after preliminary mixing is discharged from the installation shell 217. , and passes through the three connecting plates 218 in sequence. During this process, the airflow can be separated by the dividing strips 219 to form multiple airflows, and enter the inner side of the next connecting plate 218. Due to the staggered arrangement of the dividing strips 219, the separated airflow is separated again and mixed with the adjacent separated airflows to enter the inner side of the next connecting plate 218 to repeat the above steps, thereby enhancing the mixing effect of carbon dioxide and other gases. Finally, it enters the interior of the detection cavity and is discharged through the exhaust pipe 212. During this process, its concentration can be detected by the first detector 214. The carbon dioxide in the gas after multiple mixing is evenly distributed and the concentration detection is accurate, so as to facilitate the regulation of the concentration of carbon dioxide in the installation cavity.

[0029] like Figure 1-Figure 9 As shown, the inner core module 3 includes three horizontal plates 302 fixedly connected to the inner wall of the installation cavity and symmetrically distributed, the top of the horizontal plate 302 is fixedly connected with evenly distributed vertical plates 301, a mounting block 305 is fixedly connected between two adjacent vertical plates 301, a placement cavity is formed between the mounting block 305 and the horizontal plate 302, and a placement box 303 is arranged inside the placement cavity.

[0030] A stopper 304 for shielding the placement cavity is fixedly connected to one side of the placement box 303, and a baffle 306 is arranged at the bottom of the mounting block 305. Two symmetrically distributed rotating shafts are fixedly connected to the surface of the baffle 306, and the rotating shafts are rotatably connected to the inner walls of the adjacent vertical plates 301. A spring 307 is fixedly connected to the surface of the rotating shaft, and the other end of the spring 307 is fixedly connected to the inner wall of the vertical plate 301.

[0031] The injection pipe 204 extends to the interior of the mounting block 305 , and the lower end of the first nozzle 205 passes through the mounting block 305 .

[0032] Grooves 308 matching the guide shell 221 are provided on both sides of the placement box 303 , connecting holes 309 communicating with the placement cavity are provided on the inner wall of the placement box 303 , and discharge holes 310 evenly distributed and communicating with the grooves 308 are provided on the inner wall of the placement box 303 .

[0033] The arrangement of the horizontal plate 302, the vertical plate 301 and the mounting block 305 allows the placement chamber to form an independent chamber, so that when a placement box 303 is taken out separately, the carbon dioxide concentration inside other placement chambers will not be affected, avoiding the situation where the overall carbon dioxide concentration is destroyed after the traditional box door 101 is opened, and the subsequent readjustment operation is relatively cumbersome. After the placement box 303 is pulled out, the spring 307 drives the rotating shaft to rotate so that the baffle 306 blocks the placement chamber, reducing the outside air from entering the interior of the placement chamber, and maximally reducing the difficulty of regulating the carbon dioxide concentration. The mixed gas can be injected into the interior of the placement chamber through the injection tube 204 and the first nozzle 205 for biological tissue culture.

[0034] The gas introduced into the placement box 303 can be introduced into the gap between the groove 308 and the placement cavity through the discharge hole 310, and then can be introduced into the interior of the guide shell 221 through the air inlet hole 223. Since the opening position of the air inlet hole 223 is far away from the exhaust pipe 201, it is farther than the position through the first nozzle 205, so that the gas can fully cover the interior of the placement box 303 during the flow process, so that the mixed gas can be evenly distributed in the interior of the placement box 303 to reduce the concentration difference; And when the concentration of the gas entering the guide shell 221 meets the preset value requirement, the solenoid valve inside the branch pipe 220 is opened, and the solenoid valve inside the exhaust pipe 201 is closed, so that the gas can be guided back to the interior of the box 1 through the branch pipe 220. At this time, the gas passing through the branch pipe 220 can be guided back to the interior of the placement box 303 under the action of the connecting hole 309, so that the air inside the placement box 303 remains circulating, avoiding the situation in which the internal gas flow of traditional equipment is reduced when the concentration meets the standard, resulting in the carbon dioxide being deposited at the bottom due to its own heavy gravity and the concentration is reduced in other positions, so that the carbon dioxide distribution inside the placement box 303 is more uniform.

[0035] Working principle: Place the culture dish inside the placement box 303 and install it in the placement cavity. By starting the air pump 207, the air inside the installation cavity can be extracted under the action of the outlet pipe 206, the connecting pipe 202 and the exhaust pipe 201, and introduced into the interior of the connection box 208, filtered by the filter screen 209, and then introduced into the interior of the mixing tank 210. The gas injected into the mixing tank 210 passes through the guide groove 216 and passes through the connecting plate 215. In this process, the gas rises in a spiral shape. At this time, carbon dioxide is injected into the interior of the mixing tank 210 through the introduction pipe 211 and discharged through the second nozzle 213. At this time, the spiral airflow formed can enhance the mixing effect with carbon dioxide. The mixed gas after preliminary mixing enters from the bottom of the installation shell 217 and passes through the three connecting plates 217 in sequence. 18. During this process, the airflow can be separated by the dividing strips 219 to form multiple airflows, which enter the inner side of the next connecting plate 218. Due to the staggered arrangement of the dividing strips 219, the separated airflow is separated again and mixed with the adjacent separated airflows to enter the inner side of the next connecting plate 218 to repeat the above steps, thereby enhancing the mixing effect of carbon dioxide and other gases. Finally, the gas enters the interior of the detection cavity and is discharged through the exhaust pipe 212. The mixed gas can be injected into the interior of the placement cavity through the injection pipe 204 and the first nozzle 205 for biological tissue culture. During this process, the concentration can be detected by the first detector 214. The carbon dioxide in the gas after multiple mixing is evenly distributed and the concentration detection is accurate, so as to facilitate the regulation of the concentration of carbon dioxide in the installation cavity.

[0036] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A bioengineering carbon dioxide incubator, comprising a chamber (1), characterized in that: The surface of the box body (1) is provided with an installation cavity, the surface of the box body (1) is provided with a box door (101) for shielding the installation cavity, an inner core module (3) is provided inside the installation cavity, and a gas injection module (2) is provided on one side of the box body (1); The gas injection module (2) comprises an air pump (207) arranged on one side of the box body (1); an air inlet end of the air pump (207) is connected to an outlet pipe (206); the surface of the outlet pipe (206) is connected to evenly distributed connecting pipes (202); the surface of the connecting pipe (202) is connected to an exhaust pipe (201) for exhausting air from the inside of the installation cavity; the surface of the exhaust pipe (201) is connected to a branch pipe (220) connected to the inside of the box body (1); solenoid valves are arranged inside the exhaust pipe (201) and the branch pipe (220); a connecting box (208) is arranged on one side of the air pump (207); a filter screen (209) is arranged inside the connecting box (208); an air outlet end of the air pump (207) is connected to the connecting box (208); and a mixing component is arranged on one side of the connecting box (208).

2. A bioengineering carbon dioxide incubator according to claim 1, characterized in that: The gas injection module (2) further comprises a plurality of guide shells (221) arranged inside the housing (1); one end of the guide shell (221) is connected to the air extraction pipe (201); a second detector (222) is arranged on the top of the guide shell (221); a detection end of the second detector (222) extends into the interior of the guide shell (221); and evenly distributed air inlet holes (223) are provided on a side of the top of the guide shell (221) away from the air extraction pipe (201).

3. A bioengineering carbon dioxide incubator according to claim 1, characterized in that: The mixing assembly comprises a mixing tank (210) connected to one side of a connection box (208); a connection plate (215) is fixedly connected to the inner wall of the mixing tank (210); a surface of the connection plate (215) is provided with evenly distributed guide grooves (216); the arrangement of the guide grooves (216) enables gas passing through the guide grooves (216) to rise in a spiral shape.

4. A bioengineering carbon dioxide incubator according to claim 3, characterized in that: The inner wall of the mixing tank (210) is fixedly connected to a mounting shell (217) located above the connecting plate (215); the top of the mixing tank (210) is provided with an introduction pipe (211) for introducing carbon dioxide; the lower end of the introduction pipe (211) passes through the mounting shell (217) and is connected to evenly distributed second nozzles (213); the inner wall of the mounting shell (217) is fixedly connected to three connecting plates (218) connected end to end; a detection cavity is formed between the connecting plates (218) at the top and the inner wall of the mounting shell (217); the top of the mixing tank (210) is provided with a first detector (214); the detection probe of the first detector (214) extends into the interior of the detection cavity; and the top of the mixing tank (210) is provided with a discharge pipe (212) connected to the detection cavity.

5. A bioengineering carbon dioxide incubator according to claim 4, characterized in that: Evenly distributed separation strips (219) are fixedly connected to the inner side of the connection disk (218), and the separation strips (219) arranged on the middle connection disk (218) and the separation strips (219) arranged on the other two connection disks (218) are staggered.

6. A bioengineering carbon dioxide incubator according to claim 5, characterized in that: One side of the box body (1) is provided with evenly distributed installation pipes (203), one end of the discharge pipe (212) is connected to an adjacent installation pipe (203), the surface of the installation pipe (203) is connected to evenly distributed injection pipes (204), and the other end surface of the injection pipe (204) is connected to two first nozzles (205).

7. A bioengineering carbon dioxide incubator according to claim 6, characterized in that: The inner core module (3) comprises three symmetrically distributed transverse plates (302) fixedly connected to the inner wall of the installation cavity, the top of the transverse plates (302) being fixedly connected to evenly distributed vertical plates (301), a mounting block (305) being fixedly connected between two adjacent vertical plates (301), a placement cavity being formed between the mounting block (305) and the transverse plates (302), and a placement box (303) being arranged inside the placement cavity.

8. A bioengineering carbon dioxide incubator according to claim 7, characterized in that: A stopper (304) for shielding the placement cavity is fixedly connected to one side of the placement box (303); a baffle (306) is provided at the bottom of the mounting block (305); two symmetrically distributed rotating shafts are fixedly connected to the surface of the baffle (306); the rotating shafts are rotatably connected to the inner wall of the adjacent vertical plate (301); a spring (307) is fixedly connected to the surface of the rotating shaft; the other end of the spring (307) is fixedly connected to the inner wall of the vertical plate (301).

9. A bioengineering carbon dioxide incubator according to claim 8, characterized in that: The injection pipe (204) extends to the interior of the mounting block (305), and the lower end of the first nozzle (205) passes through the mounting block (305).

10. A bioengineering carbon dioxide incubator according to claim 9, characterized in that: Both sides of the placement box (303) are provided with grooves (308) matching the guide shell (221), the inner wall of the placement box (303) is provided with connecting holes (309) communicating with the placement cavity, and the inner wall of the placement box (303) is provided with evenly distributed discharge holes (310) communicating with the grooves (308).

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