Composition capable of resisting light damage as well as preparation method and application of composition

Abstract

The invention provides a composition capable of resisting light damage as well as a preparation method and application of the composition. The composition capable of resisting light damage comprises an oil phase component and a water phase component, the oil phase component comprises titanium dioxide and bisabolol in a mass ratio of 1: (10-40); the titanium dioxide is modified by stearic acid or triethoxyoctylsilane, and the secondary particle size of the modified titanium dioxide is 0.1-1 [mu] m; the water phase component comprises a sparassis crispa extract and / or thiotaurine. The water-in-oil Pickering emulsion prepared by taking the composition disclosed by the invention as a raw material not only can enable a product to exist stably and protect active ingredients, but also can be easily adsorbed on the skin surface by relatively hydrophobic particles wrapped on the surface, and when the emulsion is smeared on the skin, the particles in the emulsion are demulsified and release inner water-phase light-loss-resistant active matters, so that the light-loss-resistant effect is realized. Active matters can be better promoted to enter the stratum corneum through lipid gaps, and the permeation and effectiveness of the active ingredients on the skin are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and particularly relates to an anti-photodamage composition and a preparation method and application thereof. Background Art

[0002] Ultraviolet rays in sunlight can damage the skin. UVB (280-320nm) penetrates the epidermis and causes sunburn and cell loss of vitality, while UVA (320-400nm) penetrates the dermis and activates reactive oxygen species (ROS), which can cause skin lipid peroxidation and damage skin collagen, resulting in long-term damage such as photoaging. Therefore, sunscreen products play an important role in daily life.

[0003] CN116672277A discloses a sunscreen composition and its preparation method and application. The sunscreen composition comprises the following components in terms of 100% by weight: 20%-28% organic sunscreen, 20%-30% oil and fat, and 2%-8% emulsifier; wherein the organic sunscreen comprises water-based sunscreen and oil-based sunscreen; the water-based sunscreen comprises camphor terephthalate sulfonic acid; the oil-based sunscreen comprises diethylhexyl butyramido triazone and drometrizole trisiloxane. After the sunscreen lotion is prepared by the water-in-oil emulsification method, the sunscreen composition of the invention can have better sunscreen performance and lower skin irritation when less sunscreen is used.

[0004] CN116035948A discloses a waterproof and long-lasting film-forming cosmetic composition and its application. The cosmetic composition comprises at least one polyurethane polymer film-forming agent, at least one organic sunscreen agent, and at least one inorganic sunscreen agent. The cosmetic composition of the invention, by using a specific polyurethane, can effectively stabilize the sunscreen agent and can form a complete film without being affected by the sunscreen agent, effectively preventing the formula from being emulsified again when it encounters water after forming a film on the skin, making the physical sunscreen agent evenly distributed in the film, effectively extending the durability of the sunscreen agent, greatly enhancing the waterproof film-forming property, and at the same time effectively improving the emulsification stability of the formula, and can be widely promoted and applied.

[0005] Currently, mainstream sunscreen products mainly use a combination of inorganic sunscreens and organic sunscreens to absorb and reflect ultraviolet rays to achieve sun protection. Because they include inorganic and organic materials as well as other auxiliary functional components, the components are complex and have poor compatibility, resulting in poor stability. Therefore, more and more Pickering emulsions are used in cosmetics. Compared with traditional emulsions, Pickering emulsions have the advantages of low cost, small dosage, safety and non-toxicity. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention aims to provide an anti-photodamage composition and its preparation method and application. The water-in-oil Pickering emulsion containing anti-photodamage components prepared by using the composition as a raw material can break the emulsion and release the anti-photodamage components under light conditions, thereby protecting against ultraviolet rays and repairing photodamaged skin cells.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides an anti-photodamage composition, the anti-photodamage composition comprising an oil phase component and an aqueous phase component;

[0009] The oil phase component includes titanium dioxide and bisabolol in a mass ratio of 1:(10-40) (for example, the mass ratio of the two can be 1:10, 1:15, 1:20, 1:25, 1:30, 1:35 or 1:40, etc.);

[0010] The titanium dioxide is modified by stearic acid or triethoxyoctylsilane, and the secondary particle size of the modified titanium dioxide is 0.1-1 μm (for example, 0.1 μm, 0.2 μm, 0.3 μm, 0.4 μm, 0.5 μm, 0.6 μm, 0.7 μm, 0.8 μm or 0.9 μm, etc.);

[0011] The water phase component includes hydrangea extract and / or thiotaurine.

[0012] The hydrangea extract in the composition of the present invention contains natural veratric acid, which can prevent and resist ultraviolet-induced DNA damage, reduce and prevent ultraviolet-induced erythema, and thiotaurine inhibits inflammatory response (1L-1α), promotes FLG and LOR protein expression, and repairs skin barrier. Veratric acid and thiotaurine contain hydroxyl groups, which can be combined with titanium dioxide to form a conjugated structure, which can make the active substance more stable, thereby making its anti-damage effect better.

[0013] Preferably, the mass ratio of the oil phase component to the water phase component is 1:(2-5), for example, it can be 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5 or 1:5, etc.

[0014] Preferably, the aqueous phase component comprises hydrangea extract and thiotaurine.

[0015] Preferably, the mass ratio of the hydrangea extract to thiotaurine is 1:(1-3), for example, it can be 1:1, 1:1.1, 1:1.3, 1:1.5, 1:1.8, 1:2, 1:2.3, 1:2.5, 1:2.7 1:2.9 or 1:3, etc.

[0016] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0017] In a second aspect, the present invention provides an emulsion with anti-photodamage efficacy, wherein the emulsion is prepared using the composition described in the first aspect as a raw material, and the emulsion is prepared by a preparation method comprising the following steps:

[0018] (1) dispersing titanium dioxide in bisabolol to form an oil phase;

[0019] Stir-frying the hydrangea extract and thiotaurine in water to obtain an aqueous phase;

[0020] (2) adding the aqueous phase to the oil phase, homogenizing and emulsifying to obtain the composition having the ability to resist light damage;

[0021] The emulsion with anti-photodamage efficacy described in the present invention is prepared with the above components as raw materials, and is an oil-in-water type Pickering emulsion, wherein titanium dioxide with a specific particle size can not only make the product more stable, but also protect the active ingredients. The relatively hydrophobic particles wrapped on the surface of the emulsion are easily adsorbed on the skin surface. When applied to the skin, the particles in the emulsion break up and release the anti-photodamage active substances in the inner water phase, which can better promote the active substances to enter the stratum corneum through the lipid gaps, thereby improving the penetration and effectiveness of the active ingredients in the skin.

[0022] Preferably, the mass percentage of the hydrangea extract in step (1) in water is 1-5%, for example, it can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4% or 4.5%, 5%, etc.

[0023] Preferably, the dispersion speed in step (2) is 1000-2000 rpm (for example, 1100 rpm, 1300 rpm, 1500 rpm, 1700 rpm or 1900 rpm, etc.), and the time is 5-15 min (for example, 5 min, 7 min, 9 min, 11 min, 13 min or 15 min, etc.).

[0024] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0025] In a third aspect, the present invention provides a use of the composition with anti-photodamage efficacy as described in the first aspect or the emulsion with anti-photodamage efficacy as described in the second aspect in a sunscreen product.

[0026] Preferably, the sunscreen product includes any one of sunscreen lotion, sunscreen liquid, sunscreen cream or sunscreen spray.

[0027] In a fourth aspect, the present invention provides a sunscreen lotion, the components of which include the emulsion with anti-photodamage efficacy as described in the second aspect, zinc oxide, propylene glycol dicaprylate / dicaprate, a chemical sunscreen, disteardimonium hectorite, polydimethylsiloxane and deionized water.

[0028] Preferably, the components of the sunscreen lotion include, by weight, 1-10 parts of the emulsion with anti-photodamage efficacy as described in the second aspect, 1-20 parts of zinc oxide, 1-10 parts of propylene glycol dicaprylate / dicaprate, 1-20 parts of a chemical sunscreen, 0.1-2 parts of disteardimonium hectorite, 1-30 parts of polydimethylsiloxane and 20-99 parts of deionized water.

[0029] The amount of the emulsion with anti-photodamage effect added to the sunscreen lotion of the present invention can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts, etc.;

[0030] The amount of zinc oxide added can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts or 20 parts, etc.;

[0031] The addition amount of the propylene glycol dicaprylate / dicaprate can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts, etc.;

[0032] The amount of the chemical sunscreen added can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts or 20 parts, etc.;

[0033] The addition amount of the disteardimethylammonium hectorite can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 0.9 parts, 1 parts, 1.1 parts, 1.3 parts, 1.5 parts, 1.7 parts, 1.9 parts or 2 parts, etc.;

[0034] The amount of the polydimethylsiloxane added can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts or 30 parts, etc.;

[0035] The added amount of the deionized water can be 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, 90 parts, 95 parts or 99 parts, etc.

[0036] Preferably, the components of the sunscreen lotion include, by weight, 1-8 parts of the emulsion with anti-photodamage efficacy as described in the second aspect, 5-15 parts of zinc oxide, 1-6 parts of propylene glycol dicaprylate / dicaprate, 5-20 parts of chemical sunscreen, 0.1-1 parts of disteardimonium hectorite, 10-30 parts of polydimethylsiloxane and 20-99 parts of deionized water;

[0037] Preferably, the chemical sunscreen comprises any one of ethylhexyl methoxycinnamate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene or phenylbenzimidazole sulfonic acid, or a combination of at least two thereof.

[0038] Preferably, the chemical sunscreen is a combination of ethylhexyl methoxycinnamate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene and phenylbenzimidazole sulfonic acid.

[0039] Preferably, the mass ratio of ethylhexyl methoxycinnamate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene and phenylbenzimidazole sulfonic acid is (6-9):(1-3):(2-5):(0.5-2).

[0040] Wherein "6-9" can be 6.2, 6.5, 6.8, 7, 7.2, 7.5, 7.8, 8, 8.2, 8.5 or 8.8, etc.;

[0041] "1-3" can be 1.1, 1.3, 1.5, 1.7, 1.9, 2, 2.1, 2.3, 2.5, 2.7 or 2.9, etc.;

[0042] “2-5” can be 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5 or 4.8, etc.;

[0043] “0.5-2” can mean 0.6, 0.8, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.8 or 1.9, etc.

[0044] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0045] In a fifth aspect, the present invention provides a method for preparing the sunscreen lotion as described in the fourth aspect, the preparation method comprising mixing and homogenizing the emulsion with anti-photodamage efficacy as described in the second aspect, zinc oxide, propylene glycol dicaprylate / dicaprate, a sunscreen agent, disteardimethylammonium hectorite, and polydimethylsiloxane in deionized water to obtain the sunscreen lotion.

[0046] Preferably, the homogenization speed is 10000-20000 rpm (for example, 11000 rpm, 13000 rpm, 15000 rpm, 17000 rpm or 19000 rpm, etc.), and the time is 40-70 min (for example, 42 min, 45 min, 48 min, 50 min, 52 min, 55 min, 58 min, 60 min, 62 min, 65 min or 68 min, etc.).

[0047] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] (1) The hydrangea extract in the composition of the present invention contains natural veratric acid, which can prevent and resist UV-induced DNA damage, reduce and prevent UV-induced erythema, and thiotaurine inhibits inflammatory response (1L-1α), promotes FLG and LOR protein expression, and repairs the skin barrier. Veratric acid and thiotaurine contain hydroxyl groups, which can be combined with titanium dioxide to form a conjugated structure, which can make the active substance more stable and thus better exert its anti-damage effect.

[0050] (2) The emulsion with anti-photodamage efficacy described in the present invention is prepared with the above components as raw materials, which is an oil-in-water type Pickering emulsion, wherein titanium dioxide with a specific particle size can not only make the product more stable, but also protect the active ingredients. The relatively hydrophobic particles wrapped on the surface of the emulsion are easily adsorbed on the skin surface. When applied to the skin, the particles in the emulsion break up and release the anti-photodamage active substances in the inner water phase, which can better promote the active substances to enter the stratum corneum through the lipid gaps, thereby improving the penetration and effectiveness of the active ingredients in the skin. DETAILED DESCRIPTION

[0051] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0052] The raw materials involved in the following examples and comparative examples can be used as long as they are purchased from regular dealers.

[0053] Example 1

[0054] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the anti-photodamage composition is composed of an oil phase component and an aqueous phase component in a mass ratio of 1:3.

[0055] The oil phase component is titanium dioxide and bisabolol in a mass ratio of 1:15, wherein the titanium dioxide is modified by stearic acid, and the secondary particle size ranges from 0.1 to 1 μm after grinding and dispersion;

[0056] The aqueous phase components are hydrangea extract and thiotaurine in a mass ratio of 1:1.

[0057] An emulsion with anti-photodamage effect is prepared using the above composition as a raw material, and the preparation method of the emulsion is as follows:

[0058] (1) dispersing titanium dioxide in bisabolol to form an oil phase;

[0059] (2) stirring and dispersing the hydrangea extract and thiotaurine in water (the mass percentage of the hydrangea extract in water is 2%) to form an aqueous phase;

[0060] (3) The aqueous phase and the oil phase are mixed and dispersed at 1500 rpm for 10 min to obtain the emulsion having anti-photodamage effect.

[0061] Example 2

[0062] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the anti-photodamage composition is composed of an oil phase and an aqueous phase in a mass ratio of 1:5.

[0063] The oil phase component is titanium dioxide and bisabolol in a mass ratio of 1:10, wherein the titanium dioxide is modified by stearic acid, and the secondary particle size ranges from 0.1 to 1 μm after grinding and dispersion;

[0064] The aqueous phase components are hydrangea extract and thiotaurine in a mass ratio of 1:3.

[0065] An emulsion with anti-photodamage effect is prepared using the above composition as a raw material, and the preparation method of the emulsion is as follows:

[0066] (1) dispersing titanium dioxide in bisabolol to form an oil phase;

[0067] (2) stirring and dispersing the hydrangea extract and thiotaurine in water (the mass percentage of the hydrangea extract in water is 1%) to form an aqueous phase;

[0068] (3) adding the aqueous phase to the oil phase and dispersing at 2000 rpm for 5 minutes to obtain the composition having resistance to light damage.

[0069] Example 3

[0070] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the anti-photodamage composition is composed of an oil phase and an aqueous phase in a mass ratio of 1:2.

[0071] The oil phase component is titanium dioxide and bisabolol in a mass ratio of 1:40, wherein the titanium dioxide is modified by triethoxyoctylsilane, and the secondary particle size ranges from 0.1 to 1 μm after grinding and dispersion;

[0072] The aqueous phase components are hydrangea extract and thiotaurine in a mass ratio of 1:2.

[0073] An emulsion with anti-photodamage effect is prepared using the above composition as a raw material, and the preparation method of the emulsion is as follows:

[0074] (1) dispersing titanium dioxide in bisabolol to form an oil phase;

[0075] (2) stirring and dispersing the hydrangea extract and thiotaurine in water (the mass percentage of the hydrangea extract in water is 5%) to form an aqueous phase;

[0076] (3) adding the aqueous phase to the oil phase and dispersing at 1000 rpm for 15 minutes to obtain the composition having resistance to light damage.

[0077] Example 4

[0078] This embodiment provides a composition with resistance to light damage and an emulsion with resistance to light damage, wherein the difference between the composition and Example 1 is only that the secondary particle size of titanium dioxide after grinding and dispersion is 2-3 μm, and the amount of titanium dioxide is kept unchanged, and the other components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0079] Example 5

[0080] This embodiment provides a photodamage-resistant composition and a photodamage-resistant emulsion, wherein the composition differs from Example 1 only in that the secondary particle size of the titanium dioxide after grinding and dispersion is 0.01-0.1 μm, and the amount of titanium dioxide is kept unchanged, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0081] Example 6

[0082] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition is different from Example 1 only in that the mass ratio of titanium dioxide to bisabolol is 1:3, and the amount of the oil phase component is kept unchanged, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0083] Example 7

[0084] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition is different from Example 1 only in that the mass ratio of titanium dioxide to bisabolol is 1:60, and the amount of the oil phase component is kept unchanged, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0085] Example 8

[0086] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition differs from that in Example 1 only in that the mass ratio of the oil phase to the water phase is 1:15, and the total amount of the oil phase and water phase components is kept unchanged, and the remaining components and addition amounts are consistent with those in Example 1. The preparation method of the emulsion refers to Example 1.

[0087] Example 9

[0088] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition differs from that in Example 1 only in that the mass ratio of the oil phase to the water phase is 2:1, and the total amount of the oil phase and water phase components is kept unchanged, and the remaining components and addition amounts are consistent with those in Example 1. The preparation method of the emulsion refers to Example 1.

[0089] Example 10

[0090] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition differs from Example 1 only in that the aqueous phase component contains only hydrangea extract, and the amount of the aqueous phase component remains unchanged, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0091] Embodiment 11

[0092] This embodiment provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition differs from Example 1 only in that the aqueous phase component contains only thiotaurine, and the amount of the aqueous phase component remains unchanged, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0093] Comparative Example 1

[0094] This comparative example provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition is different from Example 1 only in that bisabolol in the oil phase is replaced by an equal amount of propylene glycol dicaprylate / dicaprate, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0095] Comparative Example 2

[0096] This comparative example provides a photodamage-resistant composition and a photodamage-resistant emulsion, wherein the composition differs from Example 1 only in that titanium dioxide is replaced by an equal amount of corn starch (modified with stearic acid, with a secondary particle size range of 0.1-1 μm after grinding and dispersion), and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0097] Comparative Example 3

[0098] This comparative example provides an anti-photodamage composition and an anti-photodamage emulsion, wherein the composition differs from Example 1 in that it only contains an aqueous phase component, and the aqueous phase component is a hydrangea extract and thiotaurine in a mass ratio of 1:1. The preparation method of the emulsion refers to Example 1.

[0099] Comparative Example 4

[0100] This comparative example provides a photodamage-resistant composition and a photodamage-resistant emulsion, wherein the composition differs from Example 1 only in that titanium dioxide is replaced by an equal amount of emulsifier EM90, and the remaining components and addition amounts are consistent with Example 1. The preparation method of the emulsion refers to Example 1.

[0101] Test Example 1: Stability Test

[0102] The emulsions prepared in Examples 1-11 and Comparative Examples 1-4 were placed in a sealed container and placed under light conditions at -15°C, 25°C, and 45°C for 1 month to observe whether there were any stratification or other stability problems. The results are shown in Table 1.

[0103] Table 1

[0104]

[0105]

[0106] As can be seen from Table 1, the emulsions of Examples 1-3 of the present invention can maintain excellent stability under different temperatures and lighting conditions, and the selection of titanium dioxide particle size, the selection and dosage of oil phase components, and the oil-water ratio directly affect the appearance stability of the emulsion. Among them, the titanium dioxide particle size in Example 5 is too small to form a stable titanium dioxide particle slurry, and thus a stable emulsion cannot be formed.

[0107] Test Example 2: Security Test

[0108] Safety evaluation:

[0109] The specific method is as follows: 40 females aged 18-35 years old with no history of allergies were selected, and each subject tried the emulsions prepared in Examples 1-3, Example 8, Examples 10-11 and Comparative Example 1 (as can be seen from Test Example 1, Examples 4-7, Example 9 and Comparative Example 2-4 will have unstable phenomena such as demulsification at room temperature 25°C or high and low temperatures and under light, so the products are judged to be unqualified, and no safety test is performed). After cleaning the back of the subject, the spot tester with the sample is applied to the selected position on the back with a non-irritating tape (the group with an equal volume of water added but no emulsion is used as a blank control group). After pasting, press lightly with your fingers to make it evenly attached to the skin for 48 hours. The subject keeps the patch area dry within 48 hours, avoids intense exercise, scratching the patch area, and long-term sunlight exposure. After 48 hours, remove the tester and mark it. After 30 minutes, when the indentation disappears, make a judgment under sufficient light.

[0110] The grading standards for skin adverse reactions are shown in Table 2:

[0111] Table 2

[0112]

[0113] The results are shown in Table 3:

[0114] Table 3

[0115]

[0116]

[0117] It can be seen from the data in Table 3 that the subjects of Examples 1-3 all had negative reactions, indicating that the product involved in the present invention has no adverse reactions to the human body and is highly safe. However, when the composition of the oil phase is not of the type specified in the present invention, the irritation of the emulsion will be increased.

[0118] Test Example 3: Light loss resistance test

[0119] Preparation of sunscreen lotion: 7 parts of the emulsion with anti-photodamage effect of Example 1, 10 parts of zinc oxide, 4 parts of propylene glycol dicaprylate / dicaprate, 8 parts of ethylhexyl methoxycinnamate, 2 parts of diethylaminohydroxybenzoyl hexyl benzoate, 3 parts of octocrylene, 1 part of phenylbenzimidazole sulfonic acid, 0.5 parts of disteardimethylammonium hectorite, and 22 parts of polydimethylsiloxane, and the balance is supplemented with deionized water to 100 parts, and mixed and homogenized at 15000 rpm in deionized water for 60 minutes to obtain the sunscreen lotion.

[0120] The above sunscreen lotion was tested for light damage resistance:

[0121] Cyclobutane pyrimidine dimer (CPD) is a photoproduct of DNA damage induced by UV irradiation and is the main marker of DNA damage. By detecting the changes in cell tissue morphology and DNA damage CPD content, the effect of the test sample on resisting UVB-induced skin damage was evaluated.

[0122] (1) The cells were divided into a blank control group, a negative control group, a positive control group and a sample group. Three parallel groups were set up in each group. The skin model was transferred to a 6-well plate, EpiGrowth culture medium (0.9 mL / well) was added, and the plate was incubated at 37°C in an incubator with saturated humidified air (relative humidity 95%) and 5% CO2 by volume;

[0123] (2) The above sunscreen solution was used as the sample group and 50 μM WY14643 was used as the positive control group. The samples were evenly applied on the surface of the skin model and incubated in a CO2 incubator (37°C, 5% CO2 by volume) for 24 h.

[0124] (3) After the incubation, except for the blank control group, the negative control group (no solution was applied), the positive control group and the sample group were placed under a UVB irradiator (irradiation dose of 600 mJ / cm 2 );

[0125] (4) After the incubation period, the test substance remaining on the surface of the model was cleaned with sterile PBS solution, and the residual liquid inside and outside the model was gently wiped with a sterile cotton swab;

[0126] (5) Histological observation of skin model and statistical analysis of results: The model used for tissue morphology detection was cut off and fixed with 4% paraformaldehyde for 24 h, then embedded in paraffin, sliced, and stained with hematoxylin-eosin (H&E) and analyzed for sunburn cells using Image-Pro Plus 6.0 software. The results are shown in Table 4.

[0127] (6) Analysis of positive expression of CPD in the epidermis: The model used for detection was cut off and fixed with 4% paraformaldehyde for 24 h, then embedded in paraffin, sliced, and stained with immunohistochemistry (IHC staining). The stained images were analyzed for DNA damage CPD content using Image-ProPlus 6.0 software. The results are shown in Table 5.

[0128] (7) Calculation of inhibition rate

[0129]

[0130] Table 4

[0131]

[0132]

[0133] Table 5

[0134] Group Relative IOD average Inhibition rate (compared with negative control group) Blank control group 1.00 / Negative control group 25.69 / Positive control group 17.30 32.66％ Sample set 1.21 95.29％

[0135] *IOD: relative integrated optical density of DNA damage CPD, which represents the content of DNA damage CPD.

[0136] It can be seen from Tables 4 and 5 that the Sunburn cells of the sunscreen lotion of Example 1 of the present invention are significantly reduced, with an inhibition rate of 100%, and the DNA damage CPD content is significantly reduced, with an inhibition rate of 95.29%, indicating that the composition with anti-light damage can better improve the anti-light damage effect of the product.

[0137] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a composition for resisting light damage and its preparation method and application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0138] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0139] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition for resisting light damage, characterized in that: The anti-photodamage composition comprises an oil phase component and an aqueous phase component; The oil phase component includes titanium dioxide and bisabolol in a mass ratio of 1:(10-40); The titanium dioxide is modified by stearic acid or triethoxyoctylsilane, and the secondary particle size of the modified titanium dioxide is 0.1-1 μm; The water phase component includes hydrangea extract and / or thiotaurine.

2. The composition for resisting light damage according to claim 1, characterized in that The mass ratio of the oil phase component to the water phase component is 1:(2-5).

3. The composition for resisting light damage according to claim 1 or 2, characterized in that: The aqueous phase components include hydrangea extract and thiotaurine; Preferably, the mass ratio of the hydrangea extract to thiotaurine is 1:(1-3).

4. An emulsion having an anti-photodamage effect, characterized in that: The emulsion is prepared using the composition according to any one of claims 1 to 3 as a raw material, and the emulsion is prepared by the following preparation method: (1) dispersing titanium dioxide in bisabolol to form an oil phase; Stir-frying the hydrangea extract and thiotaurine in water to obtain an aqueous phase; (2) The aqueous phase and the oil phase are mixed, dispersed and emulsified to obtain the emulsion with anti-photodamage effect.

5. The emulsion with anti-photodamage effect as claimed in claim 4, characterized in that: The mass percentage of the hydrangea extract in step (1) in water is 1-5%; Preferably, the dispersion speed in step (2) is 1000-2000 rpm and the dispersion time is 5-15 min.

6. Use of the anti-photodamage composition according to any one of claims 1 to 3 or the emulsion according to any one of claims 4 to 5 in sunscreen products.

7. The use according to claim 6, characterized in that The sunscreen product includes any one of sunscreen lotion, sunscreen liquid, sunscreen cream or sunscreen spray.

8. A sunscreen lotion, characterized in that: The components of the sunscreen lotion include the emulsion according to claim 4 or 5, zinc oxide, propylene glycol dicaprylate / dicaprate, chemical sunscreen, disteardimonium hectorite, polydimethylsiloxane and deionized water.

9. The sunscreen lotion according to claim 8, characterized in that: In parts by weight, the components of the sunscreen lotion include 1-10 parts of the emulsion according to claim 4 or 5, 1-20 parts of zinc oxide, 1-10 parts of propylene glycol dicaprylate / dicaprate, 1-20 parts of chemical sunscreen, 0.1-2 parts of disteardimonium hectorite, 1-30 parts of polydimethylsiloxane and 20-99 parts of deionized water; Preferably, the components of the sunscreen lotion include, by weight, 1-8 parts of the emulsion as claimed in claim 4 or 5, 5-15 parts of zinc oxide, 1-6 parts of propylene glycol dicaprylate / dicaprate, 5-20 parts of chemical sunscreen, 0.1-1 parts of disteardimonium hectorite, 10-30 parts of polydimethylsiloxane and 20-99 parts of deionized water; Preferably, the chemical sunscreen comprises any one or a combination of at least two of ethylhexyl methoxycinnamate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene or phenylbenzimidazole sulfonic acid; Preferably, the chemical sunscreen is a combination of ethylhexyl methoxycinnamate, diethylaminohydroxybenzoyl hexyl benzoate, octocrylene and phenylbenzimidazole sulfonic acid.

10. A method for preparing the sunscreen lotion according to claim 8 or 9, characterized in that: The preparation method comprises mixing the emulsion according to claim 4 or 5, zinc oxide, propylene glycol dicaprylate / dicaprate, chemical sunscreen, disteardimonium hectorite, and polydimethylsiloxane in deionized water and homogenizing to obtain the sunscreen solution; Preferably, the homogenization speed is 10000-20000 rpm, and the time is 40-70 min.

Images