Composition prepared by taking fructus chebulae and liquorice as main materials as well as preparation method and application of composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine composition preparation, particularly relates to a composition prepared by taking fructus chebulae and liquorice as main materials, and further relates to a preparation method of the composition and application of the composition in antibacterial and anti-inflammatory, anti-oxidation, whitening, anti-saccharification and antipruritic products. According to the composition disclosed by the invention, main materials comprise fructus chebulae and liquorice; the composition is obtained by crushing the fructus chebulae and the liquorice, performing enzymolysis, performing water extraction, performing concentration, performing alcohol extraction and performing purification. Based on different effects, different combination schemes of the fructus chebulae and the liquorice are provided, so that the effects of the fructus chebulae and the liquorice are more remarkable after the fructus chebulae and the liquorice are combined according to different proportions; in addition, it is also verified that after the medicine terminalia fruits and the licorice roots in different proportions are combined, certain differences exist when the medicine terminalia fruits and the licorice roots are applied to different products. On the basis that the fructus chebulae and the liquorice are combined according to a reasonable proportion, the invention further provides a preparation method of products which are suitable for the formulas and have different effects.

Description

Technical Field

[0001] The invention belongs to the technical field of preparation of traditional Chinese medicine compositions, and particularly relates to a composition prepared with terminalia chebula and liquorice as main ingredients, and also relates to a preparation method of the composition, and application of the composition in antibacterial and anti-inflammatory, anti-oxidation, whitening, anti-glycation and antipruritic products. Background Art

[0002] Terminalia chebula is a traditional Tibetan medicine that contains active ingredients such as gallic acid, chebulic acid, chebulic acid and other tannins and phenolic acids, triterpenes such as arjunin, arjunic acid, and eucomycin acid, and flavonoids such as rutin and quercetin. These active ingredients give Terminalia chebula anti-inflammatory, analgesic, antimicrobial, antioxidant, hypoglycemic, anti-cholinesterase activity, and anti-gastric ulcer effects.

[0003] Licorice is sweet and mild in nature. In addition to glycyrrhizic acid and glycyrrhizic acid glycoside, it also includes a variety of flavonoid compounds such as liquiritigenin, isoliquiritigenin, polysaccharides and other ingredients. Licorice has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, relieving pain and harmonizing various medicines. It is mainly used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough and sputum, abdominal and limb cramps and pain, carbuncle, sore and ulcer, and relieving drug toxicity. Modern pharmacological studies have shown that licorice has multiple effects such as anti-inflammatory, antiviral, antioxidant, immunomodulatory, anti-atherosclerotic, and anti-tumor.

[0004] There are currently few relevant literature reports or disclosures on the efficacy of using only these two raw materials, Terminalia chebula and Licorice. Although Terminalia chebula and Licorice each have certain anti-inflammatory, whitening or antibacterial effects, their respective effects are limited. If the two are combined, whether they will produce a synergistic effect is unknown and needs further exploration; or, after combining the two, whether they will have new effects? This is also unknown. Moreover, given that both Terminalia chebula and Licorice have multiple active ingredients, the effects of these active ingredients are not exactly the same. If the ingredients with certain specific effects of each of them can be enriched, the effect will be more significant. There is also little literature disclosure on this aspect of research. Summary of the invention

[0005] In order to solve the above technical problems, the application has conducted a detailed study on the combined extraction and use of terminalia chebula and liquorice. The results show that when the two are combined in different ratios, the effects and functions that appear are also different. Therefore, the greatest contribution of the application is to provide a formula for a composition suitable for a certain effect. For example, with regard to whitening products, the ratio of terminalia chebula and liquorice is significantly different from that of antioxidant products.

[0006] In the present invention, according to different uses of the composition, the proportions of terminalia chebula and licorice are as follows:

[0007] A composition prepared with terminalia chebula and liquorice as main ingredients, the main ingredients of the composition include terminalia chebula and liquorice; the preparation method is to grind terminalia chebula and liquorice, enzymolyze, extract with water, concentrate, decolorize, and dilute with polyol to obtain the composition product.

[0008] Although the main ingredients are both terminalia chebula and licorice, and the above method is also used to prepare the composition, when the proportion of terminalia chebula and licorice is different, the efficacy of the obtained composition product is completely different, for example:

[0009] When the composition is used to prepare an antibacterial and anti-inflammatory product, the mass ratio of Terminalia chebula to Licorice is 2.5:1;

[0010] When the composition is used to prepare an antioxidant product, the mass ratio of terminalia chebula to licorice is 5:1;

[0011] When the composition is used to prepare whitening products, the mass ratio of terminalia chebula to licorice is 1:5;

[0012] When the composition is used to prepare anti-glycation products, the mass ratio of terminalia chebula to licorice is 7:1;

[0013] When the composition is used to prepare an antipruritic product, the mass ratio of terminalia chebula to licorice is 10: 1. The key point to be protected in this application is that terminalia chebula and licorice in different ratios have different effects.

[0014] The preparation method of the above composition comprises the following steps:

[0015] S1. Grind terminalia chebula and licorice, and sieve to obtain a powder raw material; wherein the mass ratio of terminalia chebula to licorice is: 2.5:1; or 5:1; or 1:5; or 7:1; or 10:1;

[0016] S2: adding deionized water to the powdered raw material in S1, adjusting the pH value to 4.0-6.0, and soaking at 35-65°C for 30-90 minutes;

[0017] S3 adds cellulase to the soaking liquid of S2 for enzymatic hydrolysis;

[0018] S4: directly decoct and extract the enzymatic hydrolyzate in S3, filter and obtain a first filtrate; add deionized water to the filter residue, decoct and extract, filter and obtain a second filtrate; combine the first filtrate and the second filtrate, and filter through a 0.22 μm to 10 μm filter membrane to obtain an extract;

[0019] S5 concentrates the extract of S4 to obtain a concentrated solution;

[0020] S6: adding activated carbon to the concentrated solution, stirring, removing the activated carbon, and drying the obtained supernatant to obtain an extract;

[0021] S7: adding deionized water and polyol to the extract in S6, stirring to dissolve the extract, standing overnight, taking the supernatant, and drying to obtain a composition;

[0022] The amount of deionized water and polyol added is 1 to 2 times the weight of the powder raw material;

[0023] The polyol is selected from any one of 1,2-propylene glycol, 1,3-propylene glycol, butylene glycol, dipropylene glycol and glycerol.

[0024] In the above method, the preferred parameters in each step are:

[0025] S1 is passed through a 10-20 mesh sieve;

[0026] S2 uses 10wt% hydrochloric acid solution to adjust the pH value to 4.0-6.0;

[0027] S3: adding cellulase to the soaking liquid of S2, wherein the amount of cellulase added is 0.2-1.0% of the weight of the raw material of medicinal material powder; performing enzymolysis reaction in a water bath at 35-65° C. for 30-120 minutes;

[0028] In S4, the enzymatic extract of S3 is boiled and extracted for 1 to 3 hours, filtered, and the filtrate is reserved; the filter residue is then added with deionized water 5 to 20 times the mass of the medicinal material powder raw material, boiled and extracted for 1 to 3 hours, filtered, and the filtrates are combined and filtered with a 0.22 μm to 10 μm filter membrane to obtain a filtrate;

[0029] In S5, the extract is concentrated at 60 to 80° C. until a concentrated solution of 0.2 to 0.5 g crude drug / mL is obtained;

[0030] In S6, activated carbon is added at a ratio of 1 to 50 mg per liter of concentrated solution, stirred at 40 to 80°C for 2 to 5 hours, and then the activated carbon is removed and the obtained supernatant is dried to obtain an extract.

[0031] The present invention also provides the use of the above composition or the composition prepared by the above preparation method in the preparation of products with antibacterial, anti-inflammatory, anti-oxidative, whitening, antipruritic and anti-glycation effects, which are also the key contents to be protected by the present invention. For specific application effects, see the effect verification part in the examples.

[0032] For example, the mass ratio of terminalia chebula to licorice is 2.5:1, which is used in antibacterial and anti-inflammatory products. The products can be toiletries or oral hygiene products, such as facial cleansers, facial creams, sunscreens, isolation creams, toothpastes, mouth sprays, mouthwashes, etc. Of course, these products are just examples;

[0033] When the mass ratio of terminalia chebula and licorice is 5:1, it can be used in food or skin care products.

[0034] When the mass ratio of Terminalia chebula and licorice is 10:1, it can be used in anti-itch ointment products, or anti-itch washing and care products, such as anti-itch shampoo, care liquid, etc.

[0035] When the mass ratio of terminalia chebula to licorice is 1:5, the composition can be used in whitening products, such as skin care products, like face creams, facial masks and the like.

[0036] When the mass ratio of terminalia chebula to licorice is 7:1, the composition can be used in anti-glycation products.

[0037] The beneficial effects of the present invention are:

[0038] (1) The present invention provides different combinations of terminalia chebula and licorice based on different effects, so that the effects of the combinations in different ratios are more significant; and it is also verified that after terminalia chebula and licorice in different ratios are combined, they are used in different products and have certain differences; a composition with a certain effect corresponds to a specific ratio of terminalia chebula and licorice; in this application, different effects are verified through effect examples 1-6;

[0039] (2) The present application not only combines terminalia chebula and licorice in a reasonable ratio, but also provides a method for preparing products with different effects suitable for these formulas. In the present application, the active ingredients in terminalia chebula and licorice are fully dissolved by means of enzymatic hydrolysis, which helps to better exert the effects of the active ingredients in the two.

[0040] (3) Compared with the complex formula composed of dozens of medicinal materials, the formula in the present application is simple, with only two medicinal materials, which is more convenient and simple in the preparation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 The results of the inhibition zone test on Staphylococcus aureus in Effect Example 1;

[0042] Figure 2 The results of the inhibition zone test on Trichophyton mentagrophytes in Effect Example 1;

[0043] Figure 3 The results of the inhibition zone test on Candida albicans in Effect Example 1;

[0044] Figure 4 The results of the inhibition zone determination on Streptococcus mutans in Effect Example 1 are shown.

[0045] Figure 5 The results of the inhibition zone test on Porphyromonas gingivalis in effect example 1;

[0046] Figure 6 The DPPH clearance test results in effect example 2;

[0047] Figure 7 This is the ABTS clearance test result in effect example 2;

[0048] Figure 8 The results of the anti-inflammatory test in effect example 3;

[0049] Fig. 9 This is the test result of tyrosinase inhibition rate in Effect Example 4. DETAILED DESCRIPTION

[0050] In order to further illustrate the scheme and effect of the present invention, the following specific embodiments are used to illustrate.

[0051] 1. Example of Preparation Method

[0052] Example 1

[0053] The mass ratio of the main raw materials Terminalia chebula and licorice is 1:1.

[0054] The preparation method of the composition is:

[0055] S1. After crushing the terminalia chebula and liquorice respectively, the mixture was mixed in a mass ratio of 1:1, and passed through a 20-mesh sieve to obtain a powder raw material;

[0056] S2, add deionized water to the powdered raw material in S2 at a mass volume ratio of 1:20, adjust the pH value to 4.0 with 10% hydrochloric acid solution, and soak in a 50°C water bath for 60 minutes;

[0057] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added is 0.6% of the weight of the medicinal material powder raw material; enzymolysis reaction is carried out in a 50°C water bath for 90 minutes;

[0058] S4, decoct the S3 enzymatic extract for 1.5 hours, filter, and set aside the filtrate; add deionized water 20 times the mass of the medicinal material powder to the filter residue, decoct for 1.5 hours, filter, combine the filtrates, and filter with a 5 μm filter membrane to obtain a filtrate;

[0059] S5, concentrate the S4 filtrate under reduced pressure at 70°C to a drug solution concentration of 0.5 g crude drug / mL;

[0060] S6. Add 20 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 60°C for 3 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0061] S7. Add 1.5 times the amount of deionized water and 1.5 times the amount of 1,2-propylene glycol to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0062] Example 2

[0063] The mass ratio of the main raw materials, terminalia chebula and licorice, is 10:1, and can be used in the preparation of antipruritic products.

[0064] The preparation method is:

[0065] S1. Crush the terminalia chebula and licorice separately, mix them in a mass ratio of 10:1, and pass through a 10-mesh sieve to obtain a powder raw material;

[0066] S2, add deionized water to the powdered raw material in S2 at a mass volume ratio of 1:10, adjust the pH value to 5.0 with 10% hydrochloric acid solution, and soak in a 65°C water bath for 30 minutes;

[0067] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added is 0.2% of the weight of the medicinal material powder raw material; enzymolysis reaction is carried out in a 65°C water bath for 30 minutes;

[0068] S4, decoct the S3 enzymatic extract for 1 hour, filter, and set aside the filtrate; add deionized water 10 times the mass of the medicinal material powder to the filter residue, decoct for 1 hour, filter, combine the filtrates, and filter with a 10 μm filter membrane to obtain the filtrate;

[0069] S5, concentrate the S4 filtrate under reduced pressure at 60°C to a drug concentration of 0.2 g crude drug / mL;

[0070] S6. Add 50 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 80°C for 2 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0071] S7. Add deionized water twice the mass of the medicinal raw material and 1,3-propylene glycol twice the mass of the medicinal raw material to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0072] Example 3

[0073] The mass ratio of the main raw materials, terminalia chebula and licorice, is 2.5:1, and can be used in the preparation of antibacterial and anti-inflammatory products.

[0074] The preparation method of the composition is:

[0075] S1. After crushing the terminalia chebula and liquorice respectively, the mixture was mixed in a mass ratio of 2.5:1, and passed through a 20-mesh sieve to obtain a powder raw material;

[0076] S2, add deionized water to the powdered raw material in S1 at a mass volume ratio of 1:5, adjust the pH value to 4.0 with 10% hydrochloric acid solution, and soak in a 45°C water bath for 60 minutes;

[0077] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added being 1.0% of the weight of the medicinal material powder raw material; performing enzymolysis reaction in a 45°C water bath for 90 minutes;

[0078] S4, decoct the S3 enzymatic extract for 2 hours, filter, and set aside the filtrate; add deionized water 5 times the mass of the medicinal material powder to the filter residue, decoct for 2 hours, filter, combine the filtrates, and filter with a 1 μm filter membrane to obtain the filtrate;

[0079] S5, concentrate the S4 filtrate under reduced pressure at 70°C to a drug solution concentration of 0.3 g crude drug / mL;

[0080] S6. Add 20 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 60°C for 3.5 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0081] S7. Add deionized water (1 times the mass of the medicinal raw material) and butanediol (1 times the mass of the medicinal raw material) to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0082] Example 4

[0083] The mass ratio of the main raw materials, terminalia chebula and licorice, is 1:10.

[0084] The preparation method of the composition is:

[0085] S1. After crushing the terminalia chebula and liquorice respectively, the mixture was mixed in a mass ratio of 1:10, and passed through a 20-mesh sieve to obtain a powder raw material;

[0086] S2, add deionized water to the powder raw material in S1 at a mass volume ratio of 1:25, adjust the pH value to 5.5 with 10% hydrochloric acid solution, and soak in a 55°C water bath for 30 minutes;

[0087] S3, adding cellulase to the soaking solution of S2, the amount of cellulase added is 0.6% of the weight of the medicinal material powder raw material; enzymolysis reaction is carried out in a 40°C water bath for 110 minutes;

[0088] S4, decoct the S3 enzymatic extract for 1.5 hours, filter, and set aside the filtrate; add deionized water 15 times the mass of the medicinal material powder to the filter residue, decoct for 1 hour, filter, combine the filtrates, and filter with a 6 μm filter membrane to obtain the filtrate;

[0089] S5, concentrate the S4 filtrate under reduced pressure at 65°C to a drug solution concentration of 0.25 g crude drug / mL;

[0090] S6. Add 35 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 45°C for 4 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0091] S7. Add deionized water in an amount 1.5 times the mass of the medicinal raw material and dipropylene glycol in an amount 1.5 times the mass of the medicinal raw material to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0092] Example 5

[0093] The mass ratio of the main raw materials, terminalia chebula and licorice, is 1:5, and can be used in the preparation of whitening products.

[0094] The preparation method of the composition is:

[0095] S1. After crushing the terminalia chebula and liquorice respectively, the mixture was mixed in a mass ratio of 1:5, and passed through a 20-mesh sieve to obtain a powder raw material;

[0096] S2, add deionized water to the powder raw material in S1 at a mass volume ratio of 1:20, adjust the pH value to 4.5 with 10% hydrochloric acid solution, and soak in a 60°C water bath for 70 minutes;

[0097] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added is 0.3% of the weight of the medicinal material powder raw material; enzymolysis reaction is carried out in a 55°C water bath for 70 minutes;

[0098] S4, decoct the S3 enzymatic extract for 2.5 hours, filter, and set aside the filtrate; add deionized water 20 times the mass of the medicinal material powder to the filter residue, decoct for 2.5 hours, filter, combine the filtrates, and filter with a 0.45 μm filter membrane to obtain a filtrate;

[0099] S5, concentrate the S4 filtrate under reduced pressure at 75°C to a drug concentration of 0.45 g crude drug / mL;

[0100] S6. Add 25 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 75°C for 5 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0101] S7. Add deionized water and propylene glycol in an amount of 1 times the mass of the medicinal raw material to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0102] Example 6

[0103] The mass ratio of the main raw materials, terminalia chebula and licorice, is 5:1, and can be used in the preparation of antioxidant products.

[0104] The preparation method of the composition is:

[0105] S1. After crushing Terminalia chebula and licorice respectively, the mixture was mixed in a mass ratio of 5:1, and passed through a 20-mesh sieve to obtain a powder raw material;

[0106] S2, add deionized water to the powder raw material in S1 at a mass volume ratio of 1:20, adjust the pH value to 4.0 with 10% hydrochloric acid solution, and soak in a 50°C water bath for 60 minutes;

[0107] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added is 0.6% of the weight of the medicinal material powder raw material; enzymolysis reaction is carried out in a 50°C water bath for 90 minutes;

[0108] S4, decoct the S3 enzymatic extract for 1.5 hours, filter, and set aside the filtrate; add deionized water 20 times the mass of the medicinal material powder to the filter residue, decoct for 1.5 hours, filter, combine the filtrates, and filter with a 5 μm filter membrane to obtain a filtrate;

[0109] S5, concentrate the S4 filtrate under reduced pressure at 70°C to a drug solution concentration of 0.5 g crude drug / mL;

[0110] S6. Add 20 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 60°C for 3 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0111] S7. Add 1.5 times the amount of deionized water and 1.5 times the amount of 1,2-propylene glycol to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0112] Example 7

[0113] The mass ratio of the main raw materials, terminalia chebula and licorice, is 7:1, and can be used in anti-glycation products.

[0114] The preparation method is:

[0115] S1. After crushing Terminalia chebula and licorice respectively, the mixture was mixed in a mass ratio of 7:1, and passed through a 10-mesh sieve to obtain a powder raw material;

[0116] S2, add deionized water to the powdered raw material in S1 at a mass volume ratio of 1:10, adjust the pH value to 5.0 with 10% hydrochloric acid solution, and soak in a 65°C water bath for 30 minutes;

[0117] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added is 0.2% of the weight of the medicinal material powder raw material; enzymolysis reaction is carried out in a 65°C water bath for 30 minutes;

[0118] S4, decoct the S3 enzymatic extract for 1 hour, filter, and set aside the filtrate; add deionized water 10 times the mass of the medicinal material powder to the filter residue, decoct for 1 hour, filter, combine the filtrates, and filter with a 10 μm filter membrane to obtain the filtrate;

[0119] S5, concentrate the S4 filtrate under reduced pressure at 60°C to a drug concentration of 0.2 g crude drug / mL;

[0120] S6. Add 50 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 80°C for 2 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0121] S7. Add deionized water twice the mass of the medicinal raw material and 1,3-propylene glycol twice the mass of the medicinal raw material to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0122] Example 8

[0123] The mass ratio of the main raw materials, terminalia chebula and licorice, is 1:1.5.

[0124] The preparation method of the composition is:

[0125] S1. After crushing the terminalia chebula and liquorice respectively, the mixture was mixed in a mass ratio of 1:1.5, and passed through a 20-mesh sieve to obtain a powder raw material;

[0126] S2, add deionized water to the powdered raw material in S1 at a mass volume ratio of 1:5, adjust the pH value to 4.0 with 10% hydrochloric acid solution, and soak in a 45°C water bath for 60 minutes;

[0127] S3, adding cellulase to the soaking solution in S2, the amount of cellulase added being 1.0% of the weight of the medicinal material powder raw material; performing enzymolysis reaction in a 45°C water bath for 90 minutes;

[0128] S4, decoct the S3 enzymatic extract for 2 hours, filter, and set aside the filtrate; add deionized water 5 times the mass of the medicinal material powder to the filter residue, decoct for 2 hours, filter, combine the filtrates, and filter with a 1 μm filter membrane to obtain the filtrate;

[0129] S5, concentrate the S4 filtrate under reduced pressure at 70°C to a drug solution concentration of 0.3 g crude drug / mL;

[0130] S6. Add 20 mg / mL of activated carbon to the concentrated solution of S5 according to the volume of the concentrated solution, stir at 60°C for 3.5 hours, remove the activated carbon, obtain the supernatant, and dry it to obtain an extract;

[0131] S7. Add deionized water (1 times the mass of the medicinal raw material) and butanediol (1 times the mass of the medicinal raw material) to the extract obtained in S6, stir to dissolve, let stand overnight, and take the supernatant to obtain the Chinese medicine composition of the present invention.

[0132] Comparative Example 1

[0133] This comparative example provides a product made from a single prescription of Terminalia chebula. The dosage of the single prescription of Terminalia chebula used in comparative example 1 is the sum of the dosages of Terminalia chebula and licorice used in example 2. The preparation method is the same as that in example 1.

[0134] Comparative Example 2

[0135] This comparative example provides a product made from a single liquorice prescription. The amount of the single liquorice prescription used in comparative example 2 is the sum of the amounts of terminalia chebula and liquorice used in example 2. The preparation method is the same as that in example 1.

[0136] Comparative Example 3

[0137] This comparative example provides a product made from main raw materials, terminalia chebula and licorice, in a mass ratio of 1:12, and the preparation method is the same as that in Example 1.

[0138] Comparative Example 4

[0139] This comparative example provides a product made from main raw materials, terminalia chebula and licorice, in a mass ratio of 12:1. The preparation method is the same as that in Example 1.

[0140] The products of the embodiments and comparative examples are expressed in the form of data tables, as follows:

[0141] Table 1 Raw material ratios used in the examples and comparative examples

[0142]

[0143]

[0144] 2. Experimental effect verification

[0145] Effect Example 1

[0146] This effect example provides the antibacterial effect of the traditional Chinese medicine composition prepared in Examples 1 to 8 and Comparative Examples 1 to 4 of the present invention.

[0147] 1. Experimental methods

[0148] 1.1 Inhibition zone determination

[0149] The diameter of the inhibition zone of each sample was determined by the agar punching method. On the clean bench, the sterilized tryptic soy agar medium (TSA) was cooled to about 60°C, 20 mL of the culture medium was poured into the culture dish, and it was left to stand horizontally until it was completely solidified. Then an Oxford cup (6 mm inner diameter, 10 mm high) was placed on the surface of the agar culture medium, and a hole was punched with gentle pressure. The culture medium in the agar hole was picked out with a sterile needle, and 0.1 mL of the test bacterial suspension was taken on the plate, and the bacterial solution was evenly spread with a spreader to make a bacterial plate. Then the prepared samples (80 μL per well) were injected in sequence. Then the culture dish was placed in a 37°C constant temperature incubator for 24 hours, and the diameter of the inhibition zone was measured with a vernier caliper, and a 0.9% sterile sodium chloride solution was used as a blank control group.

[0150] 1.2 Minimum inhibitory concentration (MIC) determination method

[0151] The minimum inhibitory concentration of each sample was determined by double gradient dilution method. The solutions of different samples were gradient diluted with TSB liquid medium to prepare solutions of certain concentrations. The indicator bacteria in the logarithmic phase were gradient diluted with TSB liquid medium to 10 5 CFU / mL, take 100μL of bacterial solution in the 2nd to 12th wells of the 96-well plate, add 100μL of gradient dilution solution to each well, add 100μL of TSB liquid culture medium and 100μL of bacterial suspension to the first well as a blank control, and repeat the test for each strain three times. After mixing, place the 96-well plate in an incubator at 37℃ and culture for the specified time. Observe the growth of bacteria, repeat each test 3 times, and the lowest concentration of the drug solution at which no colony growth is found in the 3 test results is the MIC.

[0152] 2. Experimental results

[0153] The results of the inhibition zone determination are shown in Table 1, Table 2 and Figure 1 to Figure 5 The MIC determination results are shown in Tables 3 and 4.

[0154] Table 2 Inhibition zone determination results (n=3, )

[0155]

[0156] Note: Compared with the sample in Comparative Example 1, # P<0.05, ## P<0.01, compared with the sample of comparative example 2, & P<0.05, && P<0.01, compared with comparative sample 3, * P<0.05, ** P<0.01, compared with comparative sample 4, $ P<0.05, $$ P<0.01.

[0157] Table 3 MIC experimental determination results (n = 3)

[0158]

[0159] According to the results in Table 2, compared with Comparative Examples 1-4, the compositions of Examples 1-8 showed significant antibacterial effects on Trichophyton mentagrophytes, Candida albicans, and Streptococcus mutans (P<0.01).

[0160] Similarly, the results in Table 3 show that compared with the comparative examples, the minimum inhibitory concentrations (MIC) of the compositions of Examples 1-8 are all lower than those of the comparative examples, which indicates that these examples have significant antibacterial activity. Although the antibacterial effect of Comparative Example 1 is not significantly different from that of Example 5 in terms of Staphylococcus aureus and Porphyromonas gingivalis, this does not affect the positive evaluation of the overall antibacterial effect of the examples by the present application. Based on the results of Tables 2 and 3, the examples show significantly better effects than the comparative examples in both the diameter of the inhibition zone and the minimum inhibitory concentration of the antibacterial experiment.

[0161] As can be seen from the above data, the combination of terminalia chebula and liquorice has a synergistic effect relative to a single prescription. When the weight ratio of terminalia chebula and liquorice is within a specific range, the effect of their combination is far superior to a single prescription, and is also superior to the case outside this range. For example, in terms of antibacterial effect, when the ratio of terminalia chebula to liquorice is 2.5:1 (Example 3), its effect is superior to other examples.

[0162] Therefore, with respect to the antibacterial effect, the present invention screened out an optimized ratio scheme based on the experimental results. Under the appropriate ratio scheme, the combination of Terminalia chebula and Licorice produced the best synergistic effect.

[0163] The synergistic effect of Terminalia chebula and Licorice can be explained in depth from their respective effective chemical components and their interaction mechanisms. Terminalia chebula is mainly rich in tannins and flavonoids, which are known for their strong antioxidant and antibacterial activities, especially in destroying bacterial cell membranes and interfering with cell metabolism. The key active ingredients of Licorice are glycyrrhizic acid and glycyrrhizin. The former inhibits bacterial enzyme activity or interferes with its protein synthesis pathway, while the latter directly inhibits bacterial growth through its anti-inflammatory and antibacterial properties.

[0164] When Terminalia chebula and licorice are combined in a specific ratio, their effective chemical components act on bacterial cells through different targets, forming a multi-target synergistic effect. Terminalia chebula's polyphenolic compounds enhance the permeability of bacterial cell membranes, creating favorable conditions for the action of glycyrrhizic acid and glycyrrhizin, thereby accelerating the destruction of bacterial cells. At the same time, glycyrrhizic acid further enhances the antibacterial effect of Terminalia chebula components by inhibiting bacterial metabolism and gene expression.

[0165] Therefore, the active ingredients of Terminalia chebula and Licorice produce antibacterial effects far exceeding those of a single ingredient through multiple mechanisms of interaction, which provides a solid theoretical basis and application prospects for the development of antibacterial preparations based on natural plants. This synergistic effect not only effectively improves the antibacterial effect, but also reduces the risk of bacterial resistance to drugs, and has important scientific and practical application value.

[0166] Effect Example 2

[0167] This effect example provides the antioxidant effects of the traditional Chinese medicine compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 4 of the present invention.

[0168] 1. Experimental methods

[0169] 1.1 Effect on DPPH free radical scavenging

[0170] Weigh a certain amount of sample and dissolve it in DMSO (analytical grade), and use ultrasound to fully dissolve it. Add 80 μL of sample solutions of different concentrations and 80 μL of 1 mmol / L DPPH solution to a 96-well plate in sequence, react at room temperature and avoid light for 30 minutes, and measure its absorbance at 517 nm and record it as A1. Perform parallel measurements 3 times and take the average value. Use vitamin C as a positive control.

[0171] The calculation formula is as follows:

[0172]

[0173] A2: Replace DPPH solution with the same volume of DMSO; A0: Replace the sample with the same volume of DMSO, and the operation is the same as A1.

[0174] 1.2 About ABTS + Effects of free radical scavenging

[0175] Accurately measure 1 mL of 7.4 mmol / L ABTS solution and 2.6 mmol / L K2S2O8 solution, mix and shake well, store at 4°C in the dark for 12-16 hours to obtain ABTS stock solution, and then mix with distilled water in a certain proportion (so that the absorbance value A = 0.700 ± 0.02) to obtain ABTS working solution. Add 10 μL of different concentrations of the sample and 200 μL of ABTS working solution to a 96-well plate, time it, react at room temperature in the dark for 6 minutes, and measure the absorbance at 734 nm and record it as A1. Vitamin C is used as a positive control.

[0176] The calculation formula is as follows:

[0177]

[0178] A0: Replace the sample with the same volume of distilled water and follow the same procedure as A1.

[0179] 2. Data processing

[0180] According to the linear fitting curves of scavenging DPPH and ABTS free radicals of different sample solutions, regression analysis was performed using IBM SPSS Statistics 26 to calculate the amount of sample solution added when the original mass concentration of DPPH and ABTS was reduced to 50% (stable state), which is the half inhibition concentration (IC 50 ). According to the half inhibitory concentration (IC 50 ) can be used to determine the ability of the sample solution to scavenge free radicals. 50 The smaller the value, the stronger the free radical scavenging ability.

[0181] 3. Experimental results

[0182] 3.1 Analysis of in vitro antioxidant (DPPH, ABTS) performance

[0183] The results are as follows Figure 6-7 As shown, when the sample of Example 6 scavenges DPPH free radicals, the DPPH scavenging rate reaches 50% when the concentration is 0.24 mg / mL, while the DPPH free radical scavenging rates in the comparative samples are significantly less than 50%; when the concentration is about 3.125 mg / mL, the scavenging rate reaches a maximum value and stabilizes at about 94%, which is higher than the positive control vitamin C. When scavenging ABTS free radicals, the inhibition rate of the sample of Example 6 is stabilized at about 94% when the concentration is about 0.39 mg / mL, which is higher than the positive control. When scavenging free radicals, the other comparative samples also show an improved antioxidant effect as the sample concentration increases. However, the in vitro antioxidant effect of the comparative samples is not as good as that of the sample of Example 6.

[0184] 3.2 Analysis of in vitro antioxidant assay results

[0185] Table 4 Determination results of in vitro antioxidant activity of Examples 1-8 and Comparative Examples 1-4 (n=3, )

[0186]

[0187] Note: Compared with comparative sample 1, & P<0.05, && P<0.01, compared with comparative sample 2, # P<0.05, ## P<0.01, compared with comparative sample 3, * P<0.05, ** P<0.01, compared with comparative sample 4, $ P<0.05, $$ P<0.01.

[0188] According to the results in Table 4, it can be clearly seen that the samples of Examples 1-8 are significantly better than the comparative samples in the ability to scavenge DPPH and ABTS free radicals (P<0.01). Among them, the sample of Example 6 (the mass ratio of Terminalia chebula to Licorice is 5:1) has the best effect in the ability to scavenge DPPH and ABTS free radicals. Comprehensively considered, the samples of the examples show a relatively excellent in vitro antioxidant effect, which is far better than the comparative samples.

[0189] As can be seen from the above data, the combination of terminalia chebula and liquorice has a synergistic effect relative to a single prescription. When the weight ratio of terminalia chebula and liquorice is within a specific range, the effect of their combination is far superior to a single prescription, and is also superior to the case outside this range. For example, with regard to the antioxidant effect, when the ratio of terminalia chebula to liquorice is 5:1, its effect is superior to other examples.

[0190] The synergistic effect of myrobalan and liquorice in antioxidant effect mainly comes from the complementarity of their respective effective ingredients. The tannins and flavonoids of myrobalan can quickly remove free radicals, while the glycyrrhizic acid and glycyrrhizin of liquorice can extend the persistence of antioxidant effect by inhibiting free radical generation and enhancing the activity of cellular antioxidant enzymes. When the two are combined according to a specific ratio, not only the scavenging ability of DPPH and ABTS free radicals is significantly improved, but also the persistence and stability of the overall antioxidant effect are enhanced. The experiment verifies the optimized ratio of this combination, effectively improves the antioxidant effect, and proves the scientific value and practical application potential of the compounding of plant ingredients in the field of antioxidant. Therefore, for the antioxidant effect, the present invention has screened out an optimized ratio scheme based on the experimental results, and under an appropriate ratio scheme, the compounding of myrobalan and liquorice produces the best synergistic effect.

[0191] Effect Example 3

[0192] This effect example provides the anti-inflammatory effect of the Chinese medicine composition prepared in Examples 1 to 8 and Comparative Examples 1 to 4 of the present invention.

[0193] 1. Experimental methods

[0194] Mouse mononuclear macrophage RAW264.7 was purchased from Wuhan Pronocell Life Science Co., Ltd. RAW264.7 cells were inoculated in DMEM medium containing 10% FBS and 1% penicillin / streptomycin. RAW264.7 cells were inoculated in the anti-inflammatory test. 5The cells were inoculated with 1 μg / mL of 1 μg / mL of each sample of the present invention and each comparative example (100 μg / mL) in a 24-well plate, and blank control group, LPS group, and each drug group, positive drug group (dexamethasone) were set up. After culturing in a 5% CO2, 37°C constant temperature incubator for 12 hours, the samples of the embodiments of the present invention and each comparative example (100 μg / mL) were pre-administered for protection for 12 hours, and then lipopolysaccharide (LPS, 1 μg / mL) was used to induce RAW264.7 cells for 24 hours. After the induction, the cell culture supernatant was collected, and the generation of inflammatory factors was detected using an ELISA kit.

[0195] 2. Statistical methods

[0196] The experimental data were analyzed using SPSS22.0 statistical software. The statistics were expressed as mean ± standard deviation (X ± SD), and one-way analysis of variance was used for comparison between groups.

[0197] 3. Experimental results

[0198] The results of the proinflammatory cytokines TNF-α, IL-1β, and IL-6 are shown in Table 5.

[0199] Table 5 Inflammation index results

[0200]

[0201] Note: Compared with the blank group, ## P<0.01. Compared with the model group, ** P<0.01. Compared with comparative example 1, $ P<0.05, $$ P<0.01. Compared with Comparative Example 2, & P<0.05, && P<0.01, compared with comparative sample 3, Compared with comparative sample 4, § P<0.05, §§ P<0.01.

[0202] The results of Table 5 show that LPS can significantly stimulate the release of inflammatory factors in RAW264.7 cells. Compared with the blank group, the content of inflammatory factors (such as TNF-α, IL-1β, IL-6) increased significantly (P<0.01). In each sample treatment group, the samples of Examples 1-8 and Comparative Examples 1-4 significantly reduced the levels of these inflammatory factors, and compared with the model group, they all showed significant anti-inflammatory effects (P<0.01). It is worth noting that the effects of the samples of each embodiment in reducing the level of inflammatory factors are better than those of the comparative examples (P<0.01), indicating that the anti-inflammatory effect of the Chinese medicine composition is significantly better than that of a single prescription.

[0203] From the above data, it can be seen that the combination of terminalia chebula and liquorice has a synergistic effect relative to a single prescription. When the weight ratio of terminalia chebula and liquorice is within a specific range, the effect of their combination is far better than a single prescription, and is also better than the case outside this range. For example, in terms of anti-inflammatory effect, when the ratio of terminalia chebula to liquorice is 2.5:1 (Example 3), its effect is better than the other examples.

[0204] The synergistic effect of terminalia chebula and licorice in anti-inflammatory effects stems from the mutual cooperation of their respective anti-inflammatory components through different mechanisms. Terminalia chebula's polyphenolic compounds effectively inhibit the activation of early inflammatory signals and reduce the release of pro-inflammatory factors; licorice's glycyrrhizic acid further slows down the expansion and persistence of inflammatory responses by inhibiting NF-κB and MAPK pathways. When the two are combined in a specific ratio, they show an anti-inflammatory effect that is significantly better than either alone. The experimental results show that this combination has a synergistic effect in inhibiting inflammation at multiple targets. This optimized ratio not only enhances the anti-inflammatory effect, but also provides theoretical support and application prospects for the development of natural anti-inflammatory drugs.

[0205] Effect Example 4

[0206] This effect example provides the whitening effect (tyrosinase inhibition effect evaluation) of the traditional Chinese medicine composition prepared in Examples 1 to 8 of the present invention and Comparative Examples 1 to 4.

[0207] 1. Experimental methods

[0208] First, prepare 368U / mL tyrosinase solution and 1.0mg / mL tyrosine solution, the total reaction volume is 100μL, and the composition of the reaction solution is shown in Table 6. Use a pipette to accurately draw the substrate, solvent, and drug in a, b, c, and d and add them to the corresponding positions of the 96-well plate. Make 3 groups of each concentration in parallel, mix them evenly, and keep them warm in a constant temperature water bath at 25℃ for 10min. After dropping 368U / mL tyrosinase solution, keep the entire 96-well plate containing liquid in a constant temperature water bath at 37℃ for 20min, and then immediately put it into the microplate reader to measure the absorbance A at 475nm, and record them as Aa, Ab, Ac, Ad in turn. Inhibition rate = [1-(Ac-Ad) / (Aa-Ab)]×100%. α-Arbutin was used as the positive control.

[0209] Aa: Absorbance of the mixture of tyrosinase and no sample at 475nm

[0210] Ab: Absorbance of the mixture without sample and tyrosinase at 475nm

[0211] Ac: absorbance of the mixture of sample and tyrosinase at 475nm

[0212] Ad: Absorbance of the mixture with sample but without tyrosinase at 475nm

[0213] Table 6 Tyrosinase inhibition rate IC 50

[0214]

[0215] Note: Compared with comparative sample 1, & P<0.05, && P<0.01, compared with comparative sample 2, # P<0.05, ## P<0.01, compared with comparative sample 3, * P<0.05, ** P<0.01, compared with comparative sample 4, $ P<0.05, $$ P<0.01.

[0216] According to the data in Table 6, the samples of Examples 1-8 have significantly better inhibitory effects on tyrosinase than the samples of Comparative Examples 1-4 (P<0.01). Compared to the comparative examples, the samples of the examples show stronger tyrosinase inhibitory activity, which is reflected in the smaller IC50 value of tyrosinase inhibition. This shows that the samples of the examples have more excellent tyrosinase inhibitory effects. Therefore, the samples of the examples can be added as cosmetic raw materials, which can effectively enhance the whitening effect of the final product. In contrast, although the samples of Comparative Examples 1-2 have a certain inhibitory effect on tyrosinase, their IC50 values ​​are relatively large. If applied to the final product, it will cause increased costs and poor whitening effects.

[0217] As can be seen from the above data, the combination of terminalia chebula and liquorice has a synergistic effect relative to a single prescription. When the weight ratio of terminalia chebula and liquorice is within a specific range, the effect of their combination is far superior to the single prescription, and is also superior to the case outside this range. For example, with regard to the whitening effect, when the ratio of terminalia chebula to liquorice is 1:5 (Example 5), its effect is superior to other examples.

[0218] Effect Example 5

[0219] This effect example provides an anti-glycation effect evaluation test of the traditional Chinese medicine composition prepared in Examples 1 to 8 and Comparative Examples 1 to 4 of the present invention.

[0220] 1. Experimental methods

[0221] 1.1 Establishment of in vitro protein AGE formation system

[0222] A solution of bovine serum albumin (BSA) with a final concentration of 20 mg / mL and 0.5 mol / L glucose was prepared with a phosphate buffer with a pH of 7.4 as the matrix liquid. The above solutions were mixed at a ratio of 1:1, sterilized and filtered with a 0.45 μm filter membrane, and dispensed into 1.5 mL conical bottom centrifuge tubes. Incubate at 37°C and 60°C for 24 hours under aerobic conditions and in the dark. The OD value of the formed AGE was determined by an enzyme marker (detection wavelength 370 nm, reference wavelength 440 nm).

[0223] 1.2 In vitro determination of inhibition of AGE formation

[0224] Using the BSA-glucose simulation reaction system, Example 3, Comparative Example 1, and Comparative Example 2 were mixed with bovine serum albumin (BSA)-glucose model solution in a ratio of 1:1 in turn as the sample group (Group A); Example 3, Comparative Example 1, and Comparative Example 2 were mixed with phosphate buffer in a ratio of 1:1 in turn as the sample group (Group B); the model solution was mixed with phosphate buffer in a ratio of 1:1 as the negative control group (Group C); the phosphate buffer solution was used as the blank control group (Group D); aminoguanidine was used as the positive control, and after incubation in a 60°C constant temperature incubator in the dark for 5 hours, the reaction was terminated by placing it in a refrigerator at 4°C for 10 minutes. The fluorescence intensity was detected using a multifunctional microplate reader at an excitation wavelength of 370nm and an emission wavelength of 440. The AGEs inhibition rate was calculated according to the following formula.

[0225]

[0226] Table 7 Anti-glycation effect

[0227]

[0228] 2. Experimental results

[0229] According to the data in Table 7, the Chinese medicine compositions obtained in Examples 1-8 all exhibited good anti-glycation effects. Compared with the comparative examples, the inhibition rates of the Chinese medicine compositions obtained from the samples of the examples on AGEs were better than those of the comparative examples, indicating that the Chinese medicine compositions had excellent effects in inhibiting the generation of AGEs. This indicates that in the composition studied, the compound pairing of the two components can effectively reduce the level of AGEs, the end product of non-enzymatic glycosylation reaction, and reduce the expression level of CML, the end product of advanced glycosylation, and have significant anti-glycation effects, thereby achieving the effects of whitening and brightening the skin and delaying aging.

[0230] From the above data, it can be seen that the combination of terminalia chebula and liquorice has a synergistic effect relative to a single prescription. When the weight ratio of terminalia chebula and liquorice is within a specific range, the effect of their combination is far better than a single prescription, and is also better than the case outside this range. For example, in terms of anti-glycation effect, when the ratio of terminalia chebula to liquorice is 7:1 (Example 7), its effect is better than the other examples.

[0231] Effect Example 6

[0232] This effect example provides the antipruritic effect of the traditional Chinese medicine composition prepared in Examples 1 to 8 and Comparative Examples 1 to 4 of the present invention.

[0233] 1. Experimental methods

[0234] Mouse mast cell MC / 9 cell line was purchased from the American Model Organism Collection (ATCC). MC / 9 cell line was inoculated in IMEM medium containing 10% FBS and 1% penicillin / streptomycin. It was cultured in a cell culture incubator at 37°C and 5% CO2, and cells in the logarithmic growth phase were used for the test. In the histamine production test, cells were inoculated in 24-well plates at a density of 1×10 5 / mL and cultured for 12 hours. Then, the samples of Example 1, Example 2, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 4 (100 μg / mL) were pre-administered for 12 hours, and then propylene glycol methyl ether acetate (PMA, 50 nM) and calcium ion introduction agent A23187 (1 μM) were used to induce MC / 9 cells for 24 hours. After the induction, the cell culture supernatant was collected and the production of histamine was detected using an ELISA kit.

[0235] 2. Statistical methods

[0236] The experimental data were statistically analyzed using SPSS 22.0 statistical software, and the statistical value was expressed as mean ± standard deviation. The comparison among groups was performed by one-way analysis of variance.

[0237] 3. Experimental results

[0238] Table 8 Results of the experiment on inhibiting histamine production (n=3, )

[0239]

[0240]

[0241] Note: Compared with the blank group, ## P<0.01. Compared with the PMA / A23187 group, & P<0.05,&& P<0.01. Compared with comparative example 1, * P<0.05, ** P<0.01. Compared with Comparative Example 2, $ P<0.05, $$ P<0.01, compared with comparative sample 3, Compared with comparative sample 4, § P<0.05, §§ P<0.01.

[0242] The results in Table 8 show that PMA / A23187 stimulation significantly promoted the release of histamine in MC / 9 cells, and compared with the blank group, the histamine content increased significantly (P<0.01). In each sample treatment group, the Chinese medicine composition of Examples 1-8 significantly inhibited the release of histamine, showing a good anti-allergic effect (P<0.01). In addition, the effects of all embodiments were better than those of Comparative Examples 1-4, showing a more significant inhibitory effect.

[0243] As can be seen from the above data, the combination of terminalia chebula and liquorice has a synergistic effect relative to a single prescription. When the weight ratio of terminalia chebula and liquorice is within a specific range, the effect of their combination is far superior to a single prescription, and is also superior to cases outside this range. For example, in terms of the antipruritic effect, when the ratio of terminalia chebula to liquorice is 10:1 (Example 2), its effect is superior to other examples.

[0244] Effect Example 7

[0245] In addition to the synergistic effect of the above-mentioned effects, the combination of terminalia chebula and liquorice can also improve the stability of the effective ingredients in terminalia chebula at different pH values. This effect example provides a stability test of the changes in the content of total phenols, gallic acid, and glycyrrhizic acid under different pH conditions for the sample of Example 3 of the present invention and terminalia chebula single prescription (Comparative Example 1) and liquorice single prescription (Comparative Example 2).

[0246] 1. Experimental methods

[0247] A Hypersil BDS C18 chromatographic column (4.6×250 mm, 5 μm) was used; the high performance liquid chromatography (HPLC) detection conditions were: 0.1% phosphoric acid aqueous solution-chromatographic grade methanol as the mobile phase, gradient elution: 0-5 min, 5%-10% methanol; 5-10 min, 10%-11% methanol; 10-15 min, 11%-15% methanol; 15-20 min, 15-25% methanol; 20-30 min, 25%-40% methanol; 30-40 min, 40%-45% methanol; 40-50 min, 45%-70% methanol; 50-60 min, 70%-80% methanol; the column temperature was 25°C, the detection wavelength was 254 nm, the flow rate was 1 mL / min, and the injection volume was 20 μL.

[0248] The total phenolic content was determined using the Folin-Ciocalteu colorimetric method with gallic acid in Terminalia chebula as the standard.

[0249] 2. Experimental results

[0250] Table 9 Example 3, Terminalia chebula single total phenol, gallic acid content changes at different pH values ​​experimental results

[0251]

[0252] Table 10 Experimental results of Example 3, total phenols and gallic acid in liquorice at different pH values

[0253]

[0254] The experimental results in Tables 9-10 show that in the range of pH 5.0 to 7.5, the total phenol and gallic acid contents in Example 3 fluctuate slightly. When the pH value is 5.0, the gallic acid content in Example 3 is 10.68 mg / mL, and the total phenol content is 50.36 mg / mL; when the pH value is 7.5, the gallic acid content in Example 3 is 10.38 mg / mL, and the total phenol content is 49.01 mg / mL. The results show that the increase in pH value has little effect on the total phenol and gallic acid contents in Example 3.

[0255] However, in the single recipe of Terminalia chebula (Comparative Example 1), as the pH value increases, the total phenol and gallic acid contents decrease significantly. When the pH value is 5.0, the gallic acid content in the single recipe of Terminalia chebula is 10.29 mg / mL, and the total phenol content is 47.21 mg / mL. When the pH value is 7.5, the gallic acid content in the single recipe of Terminalia chebula is 7.07 mg / mL, and the total phenol content is 32.11 mg / mL. The results show that as the pH value increases, the stability of gallic acid and total phenol in the single recipe of Terminalia chebula is affected, and the content decreases significantly.

[0256] In the pH range of 5.0 to 7.5, the glycyrrhizic acid content in Example 3 and the single liquorice prescription (Comparative Example 2) changes slightly, indicating that the pH value fluctuates within this range and has little effect on the glycyrrhizic acid content.

[0257] The above results show that the total phenolic components of Terminalia chebula and Licorice have better stability under different pH conditions, which may be related to the interaction between the ingredients in the formula, especially the related ingredients in Licorice. These interactions may enhance the stability of the active ingredients, thereby maintaining high biological activity under different environmental conditions. This discovery is of great significance for the development of products with good stability.

[0258] These experimental results show that the Chinese medicine composition of the present invention exhibits significant synergistic effects through scientific compatibility. The antibacterial, anti-inflammatory, antioxidant, whitening, antipruritic, and anti-glycation effects of all embodiments are better than those of the comparative sample group, which shows that the present invention achieves the optimization of the relevant properties of the Chinese medicine composition during the compatibility and preparation process, and further proves the importance of the preparation method of the present invention in enhancing the antibacterial, anti-inflammatory, antioxidant, whitening, antipruritic, and anti-glycation effects of the Chinese medicine composition.

[0259] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition prepared with terminalia chebula and liquorice as main ingredients, characterized in that: The main ingredients include terminalia chebula and liquorice; the composition is obtained by crushing terminalia chebula and liquorice, enzymolysis, water extraction, concentration, decolorization, and polyol dilution; When the composition is used to prepare an antibacterial and anti-inflammatory product, the mass ratio of Terminalia chebula to Licorice is 2.5:1; When the composition is used to prepare antioxidant products, the mass ratio of terminalia chebula to licorice is 5:1; When the composition is used to prepare whitening products, the mass ratio of terminalia chebula to licorice is 1:5; When the composition is used to prepare anti-glycation products, the mass ratio of terminalia chebula to licorice is 7:1; When the composition is used to prepare an antipruritic product, the mass ratio of Terminalia chebula to Licorice is 10:

1.

2. The method for preparing the composition according to claim 1, comprising the following steps: S1. Grind the terminalia chebula and licorice, and sieve to obtain a powder raw material; wherein, The mass ratio of terminalia chebula to licorice is: 2.5:1; or 5:1; or 1:5; or 7:1; or 10:1; S2: Add deionized water to the powdered raw material in S1, adjust the pH value to 4.0-6.0, and soak at 35-65°C for 30-90 minutes; S3 adds cellulase to the soaking liquid of S2 for enzymatic hydrolysis; S4: directly decoct and extract the enzymatic hydrolyzate in S3, filter and obtain a first filtrate; add deionized water to the filter residue, decoct and extract, filter and obtain a second filtrate; combine the first filtrate and the second filtrate, and filter through a 0.22 μm to 10 μm filter membrane to obtain an extract; S5 concentrates the extract of S4 to obtain a concentrated solution; S6: adding activated carbon to the concentrated solution, stirring, removing the activated carbon, and drying the obtained supernatant to obtain an extract; S7: adding deionized water and polyol to the extract in S6, stirring to dissolve the extract, standing overnight, taking the supernatant, and drying to obtain a composition; The amount of deionized water and polyol added is 1-2 times the weight of the powder raw material; The polyol is selected from any one of 1,2-propylene glycol, 1,3-propylene glycol, butylene glycol, dipropylene glycol and glycerol.

3. The method for preparing the composition according to claim 2, characterized in that: S1 is passed through a 10-20 mesh sieve; S2 uses 10wt% hydrochloric acid solution to adjust the pH value to 4.0~6.0; S3: adding cellulase to the soaking liquid of S2, wherein the amount of cellulase added is 0.2-1.0% of the weight of the raw material of medicinal material powder; performing enzymolysis reaction in a water bath at 35-65°C for 30-120 minutes; In S4, the S3 enzymatic extract is boiled and extracted for 1 to 3 hours, filtered, and the filtrate is reserved; the filter residue is then added with deionized water 5 to 20 times the mass of the medicinal material powder raw material, boiled and extracted for 1 to 3 hours, filtered, and the filtrates are combined and filtered with a 0.22 μm to 10 μm filter membrane to obtain a filtrate; In S5, the extract is concentrated at 60-80°C until a concentrated solution of 0.2-0.5 g crude drug / mL is obtained; In S6, activated carbon is added at a ratio of 1 to 50 mg per liter of concentrated solution, stirred at 40 to 80°C for 2 to 5 hours, and then the activated carbon is removed and the obtained supernatant is dried to obtain an extract.

4. Use of the composition prepared by the method of claim 3 in the preparation of antibacterial and anti-inflammatory products, characterized in that: In the composition, the mass ratio of terminalia chebula to liquorice is 2.5:

1.

5. Use of the composition prepared by the method according to claim 3 in the preparation of antioxidant products, characterized in that: In the composition, the mass ratio of terminalia chebula to licorice is 5:

1.

6. Use of the composition prepared by the method of claim 3 in the preparation of antipruritic products, characterized in that: In the composition, the mass ratio of terminalia chebula to licorice is 10:

1.

7. Use of the composition prepared by the method of claim 3 in preparing whitening products, characterized in that: In the composition, the mass ratio of terminalia chebula to licorice is 1:

5.

8. Use of the composition prepared by the method of claim 3 in the preparation of anti-glycation products, characterized in that: In the composition, the mass ratio of terminalia chebula to licorice is 7:1.

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