Method and device for sterilizing antibiotic fermentation culture medium

Abstract

The invention relates to the technical field of fermentation culture medium sterilization, in particular to a sterilization method and device for an antibiotic fermentation culture medium, sprayed cold water falls down from top to bottom, and the cooling time is shortened; after a circuit of a second electromagnet is switched on, a vertical plate is driven to move downwards through electromagnetic induction, the vertical plate drives a connecting shaft and a ball core to rotate, a round hole of the ball core rotates to a vertical state, a ball valve is opened, purified water flows in from a water inlet pipe, and a transverse pipe and a U-shaped pipe are cleaned; after a circuit of the first electromagnet is switched on, a vertical plate is driven to move upwards through electromagnetic induction, the vertical plate drives a connecting shaft and a ball core to rotate, a round hole of the ball core rotates to a horizontal state, the ball valve is closed, and cleaning of the pipeline is stopped; the device can be used for automatically cleaning each pipeline after high-temperature sterilization, so that a static culture medium is prevented from being quickly dried at a high temperature and attached to the inner wall of the pipeline, the cleaning difficulty is reduced, meanwhile, the cleaning water consumption is reduced, the economic cost is reduced, and the working efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of fermentation medium sterilization, in particular to a sterilization method and a device for an antibiotic fermentation medium. Background Art

[0002] Antibiotics refer to a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants in the course of their lives that have anti-pathogen or other activities, and are chemical substances that can interfere with the developmental functions of other living cells. Commonly used antibiotics in clinical practice include extracts from microbial culture fluids and chemically synthesized or semi-synthesized compounds. Culture medium refers to a nutrient matrix composed of a combination of different nutrients that is used to support the growth and reproduction of microorganisms, plants, or animals (or tissues). Generally, it contains several major types of substances, such as carbohydrates, nitrogen-containing substances, inorganic salts (including trace elements), vitamins, and water. Culture medium is not only a basic substance that provides cell nutrition and promotes cell proliferation, but also a living environment for cell growth and reproduction; fermentation culture medium is used for the growth, reproduction, and synthesis of products of bacterial strains. It must not only enable the seeds to grow rapidly after inoculation and reach a certain mycelium concentration, but also enable the grown bacteria to quickly synthesize the required products. Therefore, the composition of the fermentation medium should be rich and complete, and the carbon and nitrogen sources should pay attention to the combination of fast-acting and slow-acting nutrients, use less fast-acting nutrients and more slow-acting nutrients; the appropriate carbon-nitrogen ratio should also be considered, and buffers should be added to stabilize the pH value; and there should also be growth factors required for bacterial growth and elements, precursors and promoters required for product synthesis. In addition to the elements and compounds necessary for bacterial growth, there should also be specific elements, precursors and promoters required for the products; culture medium sterilization is an essential step in antibiotic culture, and the purpose of sterilization is to kill all microorganisms in the culture medium to ensure that the experimental results are caused by the specific microorganisms introduced, rather than contamination by other microorganisms; there are three methods for culture medium sterilization: batch sterilization, continuous sterilization, and empty tank sterilization. Batch sterilization is also called real tank sterilization or real sterilization, which refers to the sterilization process in which all the prepared culture medium is transported to the fermentation reactor, steam is passed through for direct heating, and then cooled to the inoculation temperature; continuous sterilization Sterilization is also called continuous sterilization, which refers to the sterilization process of heating, maintaining insulation and cooling the culture medium in different equipment outside the fermentation reactor through a special sterilization device, and then entering the fermentation reactor; empty tank sterilization is to continuously input the prepared and preheated (60-75°C) culture medium into a heating tower heated by direct steam with a pump, so that it reaches the sterilization temperature (126-132°C) in a short time, and then enters the maintenance tank (or maintenance tube), maintains at the sterilization temperature for 5-7 minutes, and then enters the cooling tube, cools it to the inoculation temperature and directly enters the fermentation tank that has been sterilized in advance (empty tank sterilization).

[0003] After the sterilized culture medium flows into the fermentation tank, the pipes through which the culture medium flows need to be cleaned to prevent affecting the content of various substances in the next round of culture medium. Currently, most culture medium sterilization devices require manual start-up of the cleaning device to clean the pipes after sterilization. If the cleaning device cannot be started in time, the static culture medium will quickly dry out and adhere to the inner wall of the pipe at high temperature, which not only increases the difficulty of cleaning, but also requires more water and longer time for cleaning, increasing time and economic costs. Summary of the invention

[0004] The main purpose of the present invention is to provide a method and device for sterilizing an antibiotic fermentation culture medium, so as to solve the problem in the related art that if the cleaning device cannot be started in time, the culture medium will quickly dry out at high temperature and adhere to the inner wall of the pipe, which not only increases the difficulty of cleaning, but also requires more water and longer time for cleaning, increasing time and economic costs.

[0005] In order to achieve the above object, according to one aspect of the present invention, there is provided a sterilization device for an antibiotic fermentation medium, comprising:

[0006] A sterilization box, which is used to sterilize the culture medium at high temperature;

[0007] A cooling cylinder, which is fixed to the side of the sterilization box and is used to cool the sterilized culture medium;

[0008] A water inlet valve is fixedly arranged on the top of the sterilization box. The water inlet valve comprises a ball valve, a cylinder and a circuit. The circuit controls the extension or retraction of the cylinder. The circuit comprises a switch. The switch comprises two contacts, S1 and S2. A spring is arranged inside the switch. When all the culture medium flows through the switch, the switch is pressure-free. The spring bounces the switch to contact the S2 contact, controls the extension of the cylinder, drives the ball valve to rotate, opens the water inlet valve, and cleans the sterilization device. When clean water enters the water inlet valve and flows through the switch, the switch is under pressure, the spring is pressed down, the switch contacts the S1 contact, controls the retraction of the cylinder, drives the ball valve to reverse, closes the water inlet valve, and stops cleaning the sterilization device.

[0009] Furthermore, the ball valve includes a water inlet pipe, a ball core and a connecting shaft. The ball core is located inside the water inlet pipe and is rotatably connected to the water inlet pipe. Teeth are fixedly provided on the outside of the connecting shaft. The connecting shaft is fixedly connected to the side wall of the ball core. The connecting shaft passes through the water inlet pipe and is rotatably connected to the side wall of the ball core. A cylindrical hole is provided in the middle of the ball core, and water can flow through the cylindrical hole.

[0010] Furthermore, the cylinder includes a vertical plate, a piston rod, a piston head, an upper air inlet, a lower air inlet and an air valve. The vertical plate is fixedly provided with teeth on the surface adjacent to the connecting shaft. The vertical plate is meshed with the connecting shaft. The lower end of the piston rod is fixedly connected to the upper end of the vertical plate. The piston head is fixedly sleeved on the outer ring of the piston rod. The upper air inlet is arranged above the side of the cylinder, and the lower air inlet is arranged below the side of the cylinder.

[0011] Further, the air valve includes a valve stem, air port No. 1, air port No. 2, air port No. 3, air port No. 4, air port No. 5, an air dividing plate, magnet No. 1, electromagnet No. 1, magnet No. 2 and electromagnet No. 2, the air port No. 1 and air port No. 5 are located on the same side of the air valve, and air port No. 1 is located below air port No. 5, the air port No. 1 is connected to the lower air inlet, and the air port No. 5 is connected to the upper air inlet.

[0012] Furthermore, the air port No. 2, air port No. 3 and air port No. 4 are located on the same side of the air valve and are opposite to the air port No. 1 and air port No. 5, the air port No. 2 is located above the air port No. 3, and the air port No. 3 is located above the air port No. 4, the valve stem is located inside the air valve, the magnet No. 1 is fixedly arranged at the top of the valve stem, and the magnet No. 2 is fixedly arranged at the bottom of the valve stem.

[0013] Furthermore, the circuit also includes an electromagnet No. 1, an electromagnet No. 2, a power supply and a manual switch S3. The power supply, manual switch S3 and the switch are connected in series in the circuit and fixedly arranged at the bottom of the sterilization box. The electromagnet No. 1 and the electromagnet No. 2 are connected in parallel in the circuit. The electromagnet No. 1 is fixedly arranged on the inner side of the top of the air valve, and the electromagnet No. 2 is fixedly arranged on the inner side of the bottom of the air valve. When the switch has no pressure, the spring bounces the switch to contact the S2 contact, and the No. 2 electromagnet is connected to the power supply. When the switch has pressure, the spring is pressed down, the switch contacts the S1 contact, and the No. 1 electromagnet is connected to the power supply.

[0014] Furthermore, the sterilization box includes a box body, a plurality of upper partitions, a steam outlet, a steam inlet, a transverse pipe, a plurality of lower partitions, a plurality of U-shaped tubes and a culture medium inlet, the tops of the upper partitions are fixedly connected to the inner side of the top of the box body, the bottoms of the lower partitions are fixedly connected to the inner side of the bottom of the box body, and the upper partitions and the lower partitions are alternately arranged in the box body.

[0015] Furthermore, the steam inlet is fixedly arranged on the left side of the bottom of the box body, the steam outlet is fixedly arranged on the right side of the top of the box body, the culture medium inlet is fixedly arranged on the right side of the top of the box body, the U-shaped tubes are arranged in parallel in the box body and pass through all the lower partitions and upper partitions, and the front and rear ends of the U-shaped tubes are fixedly provided with cross tubes to connect several U-shaped tubes, and the culture medium inlet is connected to the cross tube at the upper end to introduce the culture medium into the U-shaped tube.

[0016] Furthermore, the cooling cylinder includes a cylinder body, a cooling water inlet, a nozzle, a spiral tube, a water pump and a spiral tube outlet. The cooling water inlet is fixedly arranged at the top of the cylinder body, the nozzle is fixedly arranged on the inner side of the top of the cylinder body, the spiral tube is arranged inside the cylinder body, the water pump is fixedly arranged at the bottom of the cylinder body to pump the liquid in the U-shaped tube into the spiral tube, and the spiral tube outlet is fixedly arranged at the top of the side wall of the cylinder body and is connected with the spiral tube to discharge the liquid in the spiral tube.

[0017] On the other hand, the present invention also provides a method for sterilizing an antibiotic fermentation medium, which is used in any of the above-mentioned sterilization devices for the antibiotic fermentation medium, comprising the following steps:

[0018] Step 1: Connect the steam to the steam inlet. After the steam enters the sterilization box, it flows from left to right through the gap between the upper partition and the lower partition, extending the distance the steam flows in the sterilization box. After the steam is cooled, it flows to the right end of the sterilization box and is discharged from the steam outlet.

[0019] Step 2: When the temperature in the box reaches a certain value, add the culture medium to be sterilized to the culture medium inlet, and close the manual switch S3 at the same time. The culture medium flows from the upper horizontal tube into each U-shaped tube. The U-shaped tube passes through the upper partition and the lower partition and contacts with the hot steam in the box. The culture medium in the U-shaped tube is sterilized by the hot steam. The sterilized culture medium flows along the U-shaped tube into the lower horizontal tube. The water pump draws the culture medium in the horizontal tube into the spiral tube for cooling. The culture medium flows from bottom to top along the spiral tube.

[0020] Step 3: Connect cold water to the cooling water inlet, and the booster pump pressurizes the cold water and sprays it out from the nozzle. The sprayed cold water falls from top to bottom, which is opposite to the flow direction of the culture medium, increasing the amount of cold water that the culture medium contacts. The cold water falls on the spiral tube, exchanges heat with the culture medium inside it, and reduces the temperature of the culture medium. The cooled culture medium flows out from the outlet of the spiral tube and enters the fermentation tank for fermentation and cultivation. After the cooling water falls to the bottom of the cylinder, it is discharged through the drain outlet of the cylinder;

[0021] Step 4: After all the culture medium flows through the switch, the switch pops up and contacts S2. The circuit of the No. 2 electromagnet is connected. The No. 2 electromagnet generates magnetism, attracting the No. 2 magnet to move downward. The No. 2 magnet drives the air distributor to move downward through the valve stem and is located between the No. 3 air port and the No. 1 air port. The compressed air enters from the No. 3 air port, is discharged through the No. 5 air port, and then enters the upper part of the cylinder through the upper air inlet, pressing the piston head downward, and the air in the lower part of the cylinder is discharged from the lower air inlet through the No. 1 air port and then from the No. 2 air port. The piston head drives the piston rod and the vertical plate to move downward, and the vertical plate drives the connecting shaft and the ball core to rotate, so that the round hole of the ball core turns to a vertical state. The ball valve opens, and pure water flows in from the water inlet pipe to clean the horizontal pipe and the U-shaped pipe.

[0022] Step 5: When the pure water flows through the switch, the switch is pressed down, contacting the S1 contact, the circuit of the No. 1 electromagnet is connected, the No. 1 electromagnet generates magnetism, attracting the No. 1 magnet to move upward, and the No. 1 magnet drives the air distributor to move upward through the valve stem, and is located between the No. 3 and No. 5 gas ports. The compressed air enters from the No. 3 gas port, is discharged through the No. 1 gas port, and then enters the lower part of the cylinder through the lower air inlet, squeezes the piston head upward, and the air in the upper part of the cylinder is discharged from the upper air inlet through the No. 5 gas port and then from the No. 4 gas port. The piston head drives the piston rod and the vertical plate to move upward, and the vertical plate drives the connecting shaft and the ball core to rotate, so that the round hole of the ball core turns to a horizontal state, the ball valve is closed, the pure water stops entering, and the cleaning is suspended;

[0023] Step 6: After the culture medium in the spiral tube has completely flowed out, remove the spiral tube outlet from the fermentation tank and connect it to the wastewater tank. The water pump draws pure water into the spiral tube to clean the spiral tube. The cleaned wastewater is discharged into the wastewater tank through the spiral tube outlet. If the discharged wastewater contains impurities, the next round of cleaning will be automatically carried out. If the discharged wastewater is relatively clean, turn off the manual switch S3, disconnect the circuit, and the cleaning is completed.

[0024] Compared with the prior art, the present invention has the following beneficial effects: after the steam enters the sterilization box, it flows from left to right through the gap between the upper partition and the lower partition, extending the distance that the steam flows in the sterilization box and shortening the preheating time of the sterilization box; the U-shaped tube passes through the upper partition and the lower partition, fully contacts with the hot steam in the box, and the culture medium in the U-shaped tube is quickly sterilized by the hot steam; the booster pump pressurizes the cold water and sprays it from the nozzle, and the sprayed cold water falls from top to bottom, which is opposite to the flow direction of the culture medium, thereby increasing the amount of cold water that the culture medium contacts and reducing the cooling time; after the circuit of the No. 2 electromagnet is connected, the vertical plate is driven to move downward by electromagnetic induction. , the vertical plate drives the connecting shaft and the ball core to rotate, so that the round hole of the ball core turns to a vertical state, the ball valve opens, and pure water flows in from the water inlet pipe to clean the horizontal pipe and the U-shaped pipe; after the circuit of the No. 1 electromagnet is connected, the vertical plate is driven to move upward through electromagnetic induction, and the vertical plate drives the connecting shaft and the ball core to rotate, so that the round hole of the ball core turns to a horizontal state, the ball valve is closed, and the cleaning of the pipeline is stopped; the device can automatically clean each pipeline after high-temperature sterilization to prevent the static culture medium from drying quickly at high temperature and adhering to the inner wall of the pipeline, thereby reducing the difficulty of cleaning, and also reducing the amount of water used for cleaning, reducing economic costs, and improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is an overall schematic diagram of the present invention;

[0026] Figure 2 It is a cross-sectional view of the present invention;

[0027] Figure 3 It is a schematic diagram of the internal structure of the present invention;

[0028] Figure 4 This is a schematic diagram of the structure of the water inlet valve of the present invention;

[0029] Figure 5 This is a schematic diagram of the structure of the gas source connected to the lower gas inlet of the present invention;

[0030] Figure 6 This is a schematic diagram of the structure of the gas source connected to the upper gas inlet of the present invention;

[0031] Figure 7 This is a circuit diagram of the present invention.

[0032] Illustration Description:

[0033] 1. Sterilization box; 11. Box body; 12. Upper partition; 13. Steam outlet; 14. Steam inlet; 15. Horizontal pipe; 16. Lower partition; 17. U-shaped pipe; 18. Culture medium inlet;

[0034] 2. Cooling cylinder; 21. Cylinder body; 22. Cooling water inlet; 23. Nozzle; 24. Spiral tube; 25. Water pump; 26. Spiral tube outlet; 27. Switch;

[0035] 3. Water inlet valve; 31. Water inlet pipe; 32. Ball core; 33. Connecting shaft; 34. Vertical plate; 35. Piston rod; 36. Piston head; 37. Upper air inlet; 38. Lower air inlet; 39. Air valve; 390. Valve stem; 391. No. 1 air inlet; 392. No. 2 air inlet; 393. No. 3 air inlet; 394. No. 4 air inlet; 395. No. 5 air inlet; 396. Air distributor; 397. No. 1 magnet; 398. No. 1 electromagnet; 399. No. 2 magnet; 3910. No. 2 electromagnet. DETAILED DESCRIPTION

[0036] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation mode, structure, characteristics and effects of the present invention are described in detail below in combination with the accompanying drawings and preferred embodiments.

[0037] See also Figures 1 to 7 , this embodiment provides a sterilization device for an antibiotic fermentation medium, comprising: a sterilization box 1, the sterilization box 1 is used for high-temperature sterilization of the medium;

[0038] A cooling cylinder 2, which is fixedly arranged on the side of the sterilization box 1 and is used to cool the sterilized culture medium;

[0039] The water inlet valve 3 is fixed on the top of the sterilization box 1. The water inlet valve 3 includes a ball valve, a cylinder and a circuit. The circuit controls the extension or retraction of the cylinder. The circuit includes a switch 27. The switch 27 includes two contacts S1 and S2. A spring is provided in the switch 27. When the culture medium flows through the switch 27 completely, the switch 27 is pressure-free. The spring bounces the switch 27 to contact the S2 contact, controls the cylinder to extend, drives the ball valve to rotate, opens the water inlet valve 3, and cleans the sterilization device. When clean water enters the water inlet valve 3 and flows through the switch 27, the switch 27 has pressure, the spring is pressed down, and the switch 27 contacts the S1 contact, controls the cylinder to retract, drives the ball valve to reverse, closes the water inlet valve 3, and stops cleaning the sterilization device.

[0040] The ball valve includes an inlet pipe 31, a ball core 32 and a connecting shaft 33. The ball core 32 is located inside the water inlet pipe 31 and is rotatably connected to the water inlet pipe 31. Teeth are fixedly arranged on the outer side of the connecting shaft 33. The connecting shaft 33 is fixedly connected to the side wall of the ball core 32. The connecting shaft 33 passes through the water inlet pipe 31 and is rotatably connected to the side wall. A cylindrical hole is provided through the middle of the ball core 32. When the cylindrical hole is in the same direction as the water inlet pipe 31, water can flow through the cylindrical hole. When the cylindrical hole is perpendicular to the water inlet pipe 31, water cannot flow through.

[0041] The cylinder includes a vertical plate 34, a piston rod 35, a piston head 36, an upper air inlet 37, a lower air inlet 38 and an air valve 39. The vertical plate 34 is fixedly provided with teeth on the adjacent surface of the connecting shaft 33. The vertical plate 34 is meshed with the connecting shaft 33. The lower end of the piston rod 35 is fixedly connected to the upper end of the vertical plate 34. The piston head 36 is fixedly sleeved on the outer ring of the piston rod 35. The upper air inlet 37 is arranged above the side of the cylinder, and the lower air inlet 38 is arranged below the side of the cylinder.

[0042] The air valve 39 includes a valve stem 390, an air port No. 1 391, an air port No. 2 392, an air port No. 393, an air port No. 4 394, an air port No. 5 395, an air dividing plate 396, a magnet No. 1 397, an electromagnet No. 1 398, a magnet No. 2 399 and an electromagnet No. 2 3910. The air port No. 1 391 and the air port No. 5 395 are located on the same side of the air valve 39, and the air port No. 1 391 is located below the air port No. 5 395. The air port No. 1 391 is connected to the lower air inlet 38, and the air port No. 5 395 is connected to the upper air inlet 37.

[0043] Air port No. 2 392, air port No. 393 and air port No. 4 394 are located on the same side of the air valve 39 and are opposite to air port No. 1 391 and air port No. 5 395. Air port No. 2 392 is located above air port No. 393, and air port No. 393 is located above air port No. 4 394. Air port No. 393 is connected to the air source to supply air to the cylinder. The valve stem 390 is located inside the air valve 39. The No. 1 magnet 397 is fixed on the top of the valve stem 390, and the No. 2 magnet 399 is fixed on the bottom of the valve stem 390.

[0044] The circuit includes an electromagnet No. 1 398, an electromagnet No. 2 3910, a power supply and a manual switch S3. The power supply, the manual switch S3 and the switch 27 are connected in series in the circuit and fixedly arranged at the bottom of the sterilization box 1. When the manual switch S3 is disconnected, the circuit is broken. Regardless of whether the switch 27 is connected to S1 or S2, the electromagnet No. 1 398 and the electromagnet No. 2 3910 have no magnetism, and the state of the water inlet valve 3 remains unchanged. The electromagnet No. 1 398 and the electromagnet No. 2 3910 are connected in parallel in the circuit. The electromagnet No. 1 398 is fixedly arranged on the inner side of the top of the air valve 39, and the electromagnet No. 2 3910 is fixedly arranged on the inner side of the bottom of the air valve 39. When the switch 27 is without pressure, the spring bounces the switch 27 up to contact the S2 contact, and the electromagnet No. 2 3910 is connected to the power supply. When the switch 27 is under pressure, the spring is pressed down, the switch 27 contacts the S1 contact, and the electromagnet No. 1 398 is connected to the power supply.

[0045] The sterilization box 1 includes a box body 11, a plurality of upper partitions 12, a steam outlet 13, a steam inlet 14, a horizontal tube 15, a plurality of lower partitions 16, a plurality of U-shaped tubes 17 and a culture medium inlet 18. The tops of the upper partitions 12 are fixedly connected to the inner side of the top of the box body 11, and the bottoms of the lower partitions 16 are fixedly connected to the inner side of the bottom of the box body 11. The upper partitions 12 and the lower partitions 16 are alternately arranged in the box body 11 to extend the steam flow and enhance the sterilization effect.

[0046] The steam inlet 14 is fixed on the left side of the bottom of the box body 11, the steam outlet 13 is fixed on the right side of the top of the box body 11, the culture medium inlet 18 is fixed on the right side of the top of the box body 11, and the U-shaped tubes 17 are arranged in parallel in the box body 11 and pass through all the lower partitions 16 and the upper partitions 12. The front and rear ends of the U-shaped tubes 17 are fixed with cross tubes 15 to connect several U-shaped tubes 17. The culture medium inlet 18 is connected to the upper end of the cross tube 15 to introduce the culture medium into the U-shaped tube 17.

[0047] The cooling cylinder 2 includes a cylinder 21, a cooling water inlet 22, a nozzle 23, a spiral tube 24, a water pump 25 and a spiral tube outlet 26. The cooling water inlet 22 is fixedly arranged at the top of the cylinder 21, and the nozzle 23 is fixedly arranged on the inner side of the top of the cylinder 21. A booster pump is arranged between the cooling water inlet 22 and the nozzle 23 to boost the cooling water in the cooling water inlet 22 and spray it out from the nozzle 23. The spiral tube 24 is arranged inside the cylinder 21, and the water pump 25 is fixedly arranged at the bottom of the cylinder 21 to pump the liquid in the U-shaped tube 17 into the spiral tube 24. The spiral tube outlet 26 is fixedly arranged at the top of the side wall of the cylinder 21 and is connected to the spiral tube 24 to discharge the liquid in the spiral tube 24.

[0048] This embodiment also provides a method for sterilizing an antibiotic fermentation medium, and a sterilizing device for an antibiotic fermentation medium, comprising the following steps:

[0049] Step 1: Connect the steam to the steam inlet 14. After the steam enters the sterilization box 1, it flows from left to right through the gap between the upper partition 12 and the lower partition 16, extending the distance the steam flows in the sterilization box 1. After the steam is cooled, it flows to the right end of the sterilization box 1 and is discharged from the steam outlet 13.

[0050] Step 2: When the temperature in the box 11 reaches a certain value, the medium to be sterilized is added to the medium inlet 18, and the manual switch S3 is closed at the same time. The medium flows from the upper transverse tube 15 into each U-shaped tube 17. The U-shaped tube 17 passes through the upper partition 12 and the lower partition 16, and contacts with the hot steam in the box 11. The medium in the U-shaped tube 17 is sterilized by the hot steam. The sterilized medium flows along the U-shaped tube 17 into the lower transverse tube 15. The water pump 25 draws the medium in the transverse tube 15 into the spiral tube 24 for cooling, and the medium flows from bottom to top along the spiral tube 24.

[0051] Step 3: connect cold water to the cooling water inlet 22, the booster pump pressurizes the cold water and sprays it from the nozzle 23, the sprayed cold water falls from top to bottom, opposite to the flow direction of the culture medium, and increases the amount of cold water that the culture medium contacts. The cold water falls on the spiral tube 24, exchanges heat with the culture medium inside it, and reduces the temperature of the culture medium. The cooled culture medium flows out from the spiral tube outlet 26 and enters the fermentation tank for fermentation culture. After the cooling water falls to the bottom of the cylinder 21, it is discharged through the drain port of the cylinder 21;

[0052] Step 4: After all the culture medium flows through the switch 27, the switch 27 pops up and contacts the S2 contact. The circuit of the No. 2 electromagnet 3910 is connected. The No. 2 electromagnet 3910 generates magnetism and attracts the No. 2 magnet 399 to move downward. The No. 2 magnet 399 drives the air distributor 396 to move downward through the valve stem 390 and is located between the No. 3 air port 393 and the No. 1 air port 391. The compressed air enters from the No. 3 air port 393 and is discharged through the No. 5 air port 395. Then it enters the upper part of the cylinder through the upper air inlet 37, presses the piston head 36 downward, and discharges the air in the lower part of the cylinder from the lower air inlet 38 through the No. 1 air port 391 and then from the No. 2 air port 392. The piston head 36 drives the piston rod 35 and the vertical plate 34 to move downward, and the vertical plate 34 drives the connecting shaft 33 and the ball core 32 to rotate, so that the round hole of the ball core 32 turns to a vertical state, the ball valve opens, and pure water flows in from the water inlet pipe 31 to clean the horizontal pipe 15 and the U-shaped pipe 17;

[0053] Step 5: When the purified water flows through the switch 27, the switch 27 is pressed down and contacts the S1 contact. The circuit of the No. 1 electromagnet 398 is connected. The No. 1 electromagnet 398 generates magnetism and attracts the No. 1 magnet 397 to move upward. The No. 1 magnet 397 drives the air distributor 396 to move upward through the valve stem 390 and is located between the No. 3 air port 393 and the No. 5 air port 395. The compressed air enters from the No. 3 air port 393, is discharged through the No. 1 air port 391, and then enters the lower part of the cylinder through the lower air inlet 38, presses the piston head 36 upward, and the air in the upper part of the cylinder is discharged from the upper air inlet 37 through the No. 5 air port 395 and then from the No. 4 air port 394. The piston head 36 drives the piston rod 35 and the vertical plate 34 to move upward. The vertical plate 34 drives the connecting shaft 33 and the ball core 32 to rotate, so that the round hole of the ball core 32 turns to a horizontal state, the ball valve is closed, the purified water stops entering, and the cleaning is suspended.

[0054] Step 6: After the culture medium in the spiral tube 24 has completely flowed out, the spiral tube outlet 26 is taken out from the fermentation tank and connected to the wastewater tank. The water pump 25 draws pure water into the spiral tube 24 to clean the spiral tube 24. The cleaned wastewater is discharged into the wastewater tank through the spiral tube outlet 26. If the discharged wastewater contains impurities, the next round of cleaning is automatically carried out. If the discharged wastewater is relatively clean, the manual switch S3 is turned off, the circuit is disconnected, and the cleaning is completed.

[0055] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A sterilization device for antibiotic fermentation medium, characterized in that: include: A sterilization box (1), wherein the sterilization box (1) is used to sterilize the culture medium at high temperature; A cooling cylinder (2), wherein the cooling cylinder (2) is fixedly arranged on the side of the sterilization box (1) and is used to cool the sterilized culture medium; A water inlet valve (3), the water inlet valve (3) is fixedly arranged on the top of the sterilization box (1), the water inlet valve (3) comprises a ball valve, a cylinder and a circuit, the circuit controls the cylinder to extend or retract, the circuit comprises a switch (27), the switch (27) comprises two contacts S1 and S2, a spring is arranged inside the switch (27), when the culture medium flows through the switch (27), the switch (27) is pressure-free, the spring bounces the switch (27) to contact the S2 contact, controls the cylinder to extend, drives the ball valve to rotate, opens the water inlet valve (3), and cleans the sterilization device, when clean water enters the water inlet valve (3) and flows through the switch (27), the switch (27) is pressured, the spring is pressed down, the switch (27) contacts the S1 contact, controls the cylinder to retract, drives the ball valve to reverse, closes the water inlet valve (3), and stops cleaning the sterilization device.

2. The sterilization device for antibiotic fermentation medium according to claim 2, characterized in that: The ball valve comprises a water inlet pipe (31), a ball core (32) and a connecting shaft (33); the ball core (32) is located inside the water inlet pipe (31) and is rotatably connected to the water inlet pipe (31); teeth are fixedly provided on the outside of the connecting shaft (33); the connecting shaft (33) is fixedly connected to the side wall of the ball core (32); the connecting shaft (33) passes through the water inlet pipe (31) and is rotatably connected to the side wall; a cylindrical hole is provided in the middle of the ball core (32), and water can pass through the cylindrical hole.

3. The sterilization device for antibiotic fermentation medium according to claim 1, characterized in that: The cylinder comprises a vertical plate (34), a piston rod (35), a piston head (36), an upper air inlet (37), a lower air inlet (38) and an air valve (39); teeth are fixedly provided on the adjacent surfaces of the vertical plate (34) and the connecting shaft (33); the vertical plate (34) is meshed with the connecting shaft (33); the lower end of the piston rod (35) is fixedly connected to the upper end of the vertical plate (34); the piston head (36) is fixedly sleeved on the outer ring of the piston rod (35); the upper air inlet (37) is arranged above the side of the cylinder; and the lower air inlet (38) is arranged below the side of the cylinder.

4. The sterilization device for antibiotic fermentation medium according to claim 3, characterized in that: The air valve (39) comprises a valve stem (390), a No. 1 air port (391), a No. 2 air port (392), a No. 3 air port (393), a No. 4 air port (394), a No. 5 air port (395), an air distributor (396), a No. 1 magnet (397), a No. 1 electromagnet (398), a No. 2 magnet (399) and a No. 2 electromagnet (3910); the No. 1 air port (391) and the No. 5 air port (395) are located on the same surface of the air valve (39), and the No. 1 air port (391) is located below the No. 5 air port (395); the No. 1 air port (391) is connected to the lower air inlet (38), and the No. 5 air port (395) is connected to the upper air inlet (37).

5. The sterilization device for antibiotic fermentation medium according to claim 4, characterized in that: The No. 2 air port (392), the No. 3 air port (393) and the No. 4 air port (394) are located on the same side of the air valve (39) and are opposite to the No. 1 air port (391) and the No. 5 air port (395). The No. 2 air port (392) is located above the No. 3 air port (393), and the No. 3 air port (393) is located above the No. 4 air port (394). The valve stem (390) is located inside the air valve (39). The No. 1 magnet (397) is fixedly arranged on the top of the valve stem (390), and the No. 2 magnet (399) is fixedly arranged on the bottom of the valve stem (390).

6. The sterilization device for antibiotic fermentation medium according to claim 1, characterized in that: The circuit also includes a No. 1 electromagnet (398), a No. 2 electromagnet (3910), a power supply and a manual switch S3. The power supply, the manual switch S3 and the switch (27) are connected in series in the circuit and fixedly arranged at the bottom of the sterilization box (1). The No. 1 electromagnet (398) and the No. 2 electromagnet (3910) are connected in parallel in the circuit. The No. 1 electromagnet (398) is fixedly arranged at the inner side of the top of the air valve (39), and the No. 2 electromagnet (3910) is fixedly arranged at the inner side of the bottom of the air valve (39). When the switch (27) is without pressure, the spring bounces the switch (27) to contact the S2 contact, and the No. 2 electromagnet (3910) is connected to the power supply. When the switch (27) is under pressure, the spring is pressed down, the switch (27) contacts the S1 contact, and the No. 1 electromagnet (398) is connected to the power supply.

7. The sterilization device for antibiotic fermentation medium according to claim 1, characterized in that: The sterilization box (1) comprises a box body (11), a plurality of upper partitions (12), a steam outlet (13), a steam inlet (14), a transverse pipe (15), a plurality of lower partitions (16), a plurality of U-shaped tubes (17) and a culture medium inlet (18), the tops of the upper partitions (12) are fixedly connected to the inner side of the top of the box body (11), the bottoms of the lower partitions (16) are fixedly connected to the inner side of the bottom of the box body (11), and the upper partitions (12) and the lower partitions (16) are alternately arranged in the box body (11).

8. The sterilization device for antibiotic fermentation medium according to claim 7, characterized in that: The steam inlet (14) is fixedly arranged on the left side of the bottom of the box body (11), the steam outlet (13) is fixedly arranged on the right side of the top of the box body (11), the culture medium inlet (18) is fixedly arranged on the right side of the top of the box body (11), the U-shaped tubes (17) are arranged in parallel in the box body (11) and pass through all the lower partitions (16) and the upper partitions (12), the front and rear ends of the U-shaped tubes (17) are fixedly provided with transverse tubes (15) to connect the multiple U-shaped tubes (17), the culture medium inlet (18) is connected to the upper end of the transverse tube (15), and the culture medium is introduced into the U-shaped tube (17).

9. The sterilization device for antibiotic fermentation medium according to claim 1, characterized in that: The cooling cylinder (2) comprises a cylinder (21), a cooling water inlet (22), a nozzle (23), a spiral tube (24), a water pump (25) and a spiral tube outlet (26); the cooling water inlet (22) is fixedly arranged at the top of the cylinder (21); the nozzle (23) is fixedly arranged at the inner side of the top of the cylinder (21); the spiral tube (24) is arranged inside the cylinder (21); the water pump (25) is fixedly arranged at the bottom of the cylinder (21) to pump the liquid in the U-shaped tube (17) into the spiral tube (24); the spiral tube outlet (26) is fixedly arranged at the top of the side wall of the cylinder (21) and is connected to the spiral tube (24) to discharge the liquid in the spiral tube (24).

10. A method for sterilizing an antibiotic fermentation medium, used in the sterilizing device for an antibiotic fermentation medium according to any one of claims 1 to 9, characterized in that: The steps include: Step 1: Connect steam to the steam inlet (14). After the steam enters the sterilization box (1), it flows from left to right through the gap between the upper partition (12) and the lower partition (16), extending the distance the steam flows in the sterilization box (1). After the steam is cooled, it flows to the right end of the sterilization box (1) and is discharged from the steam outlet (13). Step 2: When the temperature in the box (11) reaches a certain value, the culture medium to be sterilized is added to the culture medium inlet (18), and the manual switch S3 is closed at the same time. The culture medium flows from the upper transverse tube (15) into each U-shaped tube (17). The U-shaped tube (17) passes through the upper partition (12) and the lower partition (16) and contacts the hot steam in the box (11). The culture medium in the U-shaped tube (17) is sterilized by the hot steam. The sterilized culture medium flows along the U-shaped tube (17) into the lower transverse tube (15). The water pump (25) draws the culture medium in the transverse tube (15) into the spiral tube (24) for cooling. The culture medium flows from bottom to top along the spiral tube (24). Step 3: Connect cold water to the cooling water inlet (22), and the booster pump pressurizes the cold water and sprays it out from the nozzle (23). The sprayed cold water falls from top to bottom, which is opposite to the flow direction of the culture medium, so as to increase the amount of cold water that contacts the culture medium. The cold water falls on the spiral tube (24), exchanges heat with the culture medium inside it, and reduces the temperature of the culture medium. The cooled culture medium flows out from the spiral tube outlet (26) and enters the fermentation tank for fermentation culture. After the cooling water falls into the bottom of the cylinder (21), it is discharged through the drain outlet of the cylinder (21); Step 4: After the culture medium has completely flowed through the switch (27), the switch (27) pops up and contacts the S2 contact. The circuit of the No. 2 electromagnet (3910) is connected. The No. 2 electromagnet (3910) generates magnetism, attracting the No. 2 magnet (399) to move downward. The No. 2 magnet (399) drives the air distributor (396) to move downward through the valve stem (390) and is located between the No. 3 air port (393) and the No. 1 air port (391). The compressed air enters from the No. 3 air port (393), is discharged through the No. 5 air port (395), and then passes through the upper The air inlet (37) enters the upper part of the cylinder and presses the piston head (36) downward, so that the air in the lower part of the cylinder is discharged from the lower air inlet (38) through the No. 1 air port (391) and then from the No. 2 air port (392). The piston head (36) drives the piston rod (35) and the vertical plate (34) to move downward, and the vertical plate (34) drives the connecting shaft (33) and the ball core (32) to rotate, so that the circular hole of the ball core (32) turns to a vertical state, and the ball valve opens, and pure water flows in from the water inlet pipe (31) to clean the horizontal pipe (15) and the U-shaped pipe (17); Step 5: When the pure water flows through the switch (27), the switch (27) is pressed down and contacts the S1 contact. The circuit of the No. 1 electromagnet (398) is connected. The No. 1 electromagnet (398) generates magnetism and attracts the No. 1 magnet (397) to move upward. The No. 1 magnet (397) drives the air distributor (396) to move upward through the valve stem (390) and is located between the No. 3 air port (393) and the No. 5 air port (395). The compressed air enters from the No. 3 air port (393) and passes through the No. 1 air port (395). 91), and then enters the lower part of the cylinder through the lower air inlet (38), presses the piston head (36) upward, and the air in the upper part of the cylinder is discharged from the upper air inlet (37) through the No. 5 air port (395) and then from the No. 4 air port (394). The piston head (36) drives the piston rod (35) and the vertical plate (34) to move upward, and the vertical plate (34) drives the connecting shaft (33) and the ball core (32) to rotate, so that the round hole of the ball core (32) turns to a horizontal state, the ball valve is closed, the pure water stops entering, and the cleaning is suspended; Step 6: After the culture medium in the spiral tube (24) has completely flowed out, the spiral tube outlet (26) is removed from the fermentation tank and connected to the wastewater tank. The water pump (25) draws pure water into the spiral tube (24) to clean the spiral tube (24). The cleaned wastewater is discharged into the wastewater tank through the spiral tube outlet (26). If the discharged wastewater contains impurities, the next round of cleaning is automatically carried out. If the discharged wastewater is relatively clean, the manual switch S3 is turned off, the circuit is disconnected, and the cleaning is completed.

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