Micro-fluidic chip for primary screening of blood donation

Abstract

The invention discloses a micro-fluidic chip for primary screening of blood donation, and relates to the technical field of micro-fluidic chips. The method is used for solving the problems of inaccurate primary screening item result, complex operation, repeated sample adding, large blood use amount and high cost of current blood donors. Comprising a chip body, and a series of operations of sample adding, measuring, mixing, reacting and detecting are integrated into the chip through unique design of an internal structure; biochemical items, infectious disease items and blood type identification of primary screening of blood donation are integrated in the chip, the chip can be detected after being added with samples once in use, and the chip has the characteristics of simple process, simplicity in operation, high precision, complete items and no need of operation of professional inspectors. And the integration and miniaturization of the detection system are realized by matching with a miniature detection instrument with small volume and low cost. The chip adopts a fan-shaped structure, the fan-shaped angle can be adjusted according to the requirements of detection items, and a plurality of samples can be detected on a machine at a time.

Description

Technical Field

[0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip for primary screening of blood donations. Background Art

[0002] At present, the initial screening of street blood donors involves biochemical items, rapid screening of infectious diseases and blood type identification, which are tested separately using dry biochemical, gold-label products and blood type reagents. There are the following defects: dry biochemical has high requirements for operation, and the repeatability and accuracy of the results are not high. Infectious diseases and blood type items are tested independently, which has the problems of complex operation and large blood collection volume. Summary of the invention

[0003] The purpose of the present invention is to solve the problems of inaccurate results of the initial screening items of blood donors, complicated operation, multiple sample addition, large amount of blood and high cost, and propose a microfluidic chip for initial screening of blood donations; the present invention integrates a series of operations of sample addition, measurement, mixing, reaction and detection into the chip through the unique design of the internal structure; and integrates the biochemical items, infectious disease items and blood type identification of the initial screening of blood donations into the chip. When in use, the chip can be tested by putting it into the machine after one sample addition, which has the characteristics of simple process, simple operation, high precision, full items and no need for professional inspectors to operate. With a small size and low cost micro-detection instrument, the integration and miniaturization of the detection system are realized. The chip adopts a fan-shaped structure, and the fan-shaped angle can be adjusted according to the needs of the detection items, so that multiple samples can be detected on the machine at one time.

[0004] The object of the present invention can be achieved through the following technical scheme: a microfluidic chip for blood donation screening, comprising a chip body, the chip body is a fan-shaped structure, and a liquid capsule placement area, a diluent fixed volume area, a sample one and diluent mixing area, a sample filling area, a sample fixed volume and separation area, a sample two fixed volume area and an infectious disease reaction area are provided on the chip body; the liquid capsule placement area is located at one end of the chip body, the diluent fixed volume area and the sample filling area are arranged on one side of the liquid capsule placement area, the sample one and diluent mixing area is located on the other side of the diluent fixed volume area, the sample fixed volume and separation area and the sample two fixed volume area are both arranged on one side of the sample filling area, and the sample fixed volume and separation area is adjacent to the sample two fixed volume area, a waste liquid pool one is provided on one side of the sample two fixed volume area, and an infectious disease reaction area is provided on one side of the waste liquid pool one.

[0005] As a preferred embodiment of the present invention, a waste liquid pool 2 and multiple sample 1 reaction holes are arranged on one side of the sample 1 and diluent mixing area, a waste liquid pool 3 is arranged corresponding to one side of the liquid sac placement area and the diluent constant volume area, and a sample 2 reaction hole and two blood type reaction holes are arranged between the waste liquid pool 2 and the infectious disease reaction area.

[0006] As a preferred embodiment of the present invention, a connecting pipe is arranged between the waste liquid pool 2 and the sample 1 and diluent mixing area, the connecting pipe is connected to the diluent constant volume area through microfluidic channel 1, the connecting pipe is connected to the sample constant volume and separation area through microfluidic channel 2, the connecting pipe is connected to the sample 1 and diluent mixing area through microfluidic channel 3, and the connecting pipe is connected to the sample 2 constant volume area through microfluidic channel 4.

[0007] As a preferred embodiment of the present invention, the number of the sample one reaction wells is three, and the three sample one reaction wells, waste liquid pool two, sample two reaction wells, two blood type reaction wells and the infectious disease reaction area are all connected to the connecting pipeline.

[0008] As a preferred embodiment of the present invention, the specific working method of the chip includes the following steps:

[0009] Step 1: Pre-place a liquid capsule in the liquid capsule placement area;

[0010] Step 2: pre-place reagents in the sample 1 reaction well, the sample 2 reaction well and the blood type reaction well;

[0011] Step 3: Add sample into the sample filling area;

[0012] Step 4: Place the chip body into the instrument and puncture the liquid capsule with the instrument;

[0013] Step 5: Centrifuge the diluent to the diluent constant volume area, and at the same time centrifuge the sample to the sample constant volume and separation area and the sample second constant volume area, and the plasma and red blood cells in the sample constant volume and separation area will be separated after centrifugation;

[0014] Step 6: Stop the centrifugation and pause for a few seconds. At this time, the liquids in microchannel 1, microchannel 2 and microchannel 4 will fill the entire microchannel under capillary action;

[0015] Step 7: When the microchannel is filled with liquid, centrifugation continues. At this time, the diluent in the diluent constant volume area and the plasma separated in the sample constant volume and separation area will be centrifuged to the sample 1 and diluent mixing area for mixing. At the same time, the sample in the sample 2 constant volume area will be centrifuged to the sample 2 reaction well, the blood type reaction well and the infectious disease reaction area to react with the preset reagents.

[0016] Step 8: When the sample 1 is fully mixed with the liquid in the diluent mixing zone, the centrifugation is stopped and paused for a few seconds to allow the microchannel 3 to be filled with liquid;

[0017] Step 9: When the microchannel 3 is filled with liquid, continue centrifugation. At this time, the liquid in the mixed area of ​​sample 1 and diluent will be distributed to the sample 1 reaction well and the sample 2 reaction well to react with the preset reagent. At the same time, the instrument collects reaction data and finally obtains the test result.

[0018] Step 10: Drain the excess liquid from each step into the corresponding waste liquid pool 1, waste liquid pool 2 and waste liquid pool 3.

[0019] As a preferred embodiment of the present invention, a QR code area is installed on the bottom surface of the chip body. The blood donor logs in to the cloud platform to register by scanning the QR code in the QR code area through the smart terminal. The cloud platform obtains the authorization of the blood donor and collects the personnel information of the authorized person, wherein the personnel information includes dietary data and work and rest data; the dietary data includes the names of foods and dietary times within a preset time period; the work and rest data includes several rest time periods and age within the preset time period; the personnel information is processed to obtain the initial screening value and stored; the instrument recognizes the QR code in the QR code area to obtain the initial screening value of the corresponding blood donor and displays it. If the initial screening value is greater than the set initial screening threshold, the chip body is associated with the blood donor, and the test results are displayed and stored in the cloud platform; if the initial screening value or the set initial screening threshold is exceeded, an early warning prompt is issued.

[0020] As a preferred implementation of the present invention, the specific process of processing personnel information is as follows:

[0021] Processing the dietary data: matching the food name with a number of preset names, where each name corresponds to a preset food shadow value, summing up all the food shadow values ​​that match the food name in the dietary data to obtain the total food shadow value; sorting the dietary times in chronological order, calculating the duration between two adjacent dietary times to obtain the dietary interval duration, determining whether the food names corresponding to the two dietary times match the food shadow value, if both match the food shadow value, summing up the values ​​of the two food shadow values ​​and dividing them by the value corresponding to the dietary interval duration to obtain the dietary screening base value , sum up all the basic values ​​of food screening to get the total value of food screening, normalize the total value of food shadow and the total value of food screening and take the value between them, set the weight coefficients corresponding to the total value of food shadow and the total value of food screening, multiply the total value of food shadow and the total value of food screening by the corresponding weight coefficients respectively, and then sum them up to get the blood donor's food anomaly value; when the food anomaly value is greater than the set food anomaly threshold, a non-compliant blood donation reminder label is generated and sent to the smart terminal of the blood donor, and the association with the scanned QR code is removed; when the food anomaly value is less than or equal to the food anomaly threshold, the work and rest data is processed, and the specific process is as follows:

[0022] The rest time period is parsed to obtain the initial rest time, the end rest time and the rest duration, and the preset rest duration threshold corresponding to the age is obtained. The parsed rest duration is compared with the rest duration threshold. If the rest duration is less than the rest duration threshold, the difference between the two is calculated to obtain the low threshold duration, and all the values ​​of the low threshold duration are summed to obtain the total rest threshold value. The initial rest time and the end rest time are matched with several influencing time periods respectively, where each influencing time period corresponds to an end-of-life value; all the matched end-of-life values ​​are summed to obtain the total end-of-life value; the number of rest durations corresponding to the rest duration less than the rest duration threshold is counted to obtain the low threshold number, the low threshold number, the total rest threshold value and the total end-of-life value are normalized and the values ​​of the three are taken, and substituted into the end-of-life model to output the end-of-life value of the blood donor, where the end-of-life model is:

[0023] dyi, xyi, ayi are the values ​​corresponding to the number of low thresholds, the total value of rest thresholds and the total value of shadows, respectively; i represents the number of blood donors, XYi is the value of shadows, μ1, μ2 and μ3 are the weight factors corresponding to the number of low thresholds, the total value of rest thresholds and the total value of shadows, respectively;

[0024] When the outlier value is greater than or equal to the set outlier threshold, a non-compliant blood donation reminder label is generated and sent to the smart terminal of the blood donor, and the association with the scanned QR code is canceled; when the outlier value is less than the set outlier threshold, the food outlier value and the outlier value are normalized and the value between the two is taken, and the values ​​of the two are converted into straight line lengths according to a certain ratio, and a circle is constructed with the straight line length of the food outlier value as the radius, and the straight line length of the circle is used as the height of the circle, and then a cone is constructed with the circle and the height, the volume of the cylinder is calculated, and the value of the volume is taken, and the preset fixed value is divided by the value of the volume to obtain the initial screening value of the blood donor.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. The present invention integrates a series of operations such as sample addition, measurement, mixing, reaction and detection into the chip through the unique design of the internal structure; and integrates the biochemical items, infectious disease items and blood type identification of the initial screening of blood donation into the chip. When in use, the chip can be tested after adding the sample once and then put into the machine. It has the characteristics of simple process, simple operation, high precision, full items and no need for professional inspectors to operate. With the small size and low cost micro-detection instrument, the integration and miniaturization of the detection system are realized.

[0027] 2. The present invention adopts a fan-shaped structure, and the fan-shaped angle can be adjusted according to the requirements of the detection project, so that multiple samples can be detected at one time.

[0028] 3. The present invention analyzes personnel information and combines the blood donors' food anomaly values ​​and boiling anomaly values ​​to obtain the initial screening value of the blood donors, and issues early warning prompts based on the initial screening value to prevent the blood donors from meeting the corresponding indicators, resulting in deviations or inaccuracies in the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to facilitate understanding by those skilled in the art, the present invention is further described below with reference to the accompanying drawings.

[0030] Figure 1 It is a schematic diagram of the overall structure of the chip of the present invention;

[0031] Figure 2 It is a schematic diagram of the microfluidic channel structure of the present invention;

[0032] Figure 3 This is a schematic diagram of the QR code area arrangement of the present invention; DETAILED DESCRIPTION

[0033] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Embodiment 1:

[0035] See also Figure 1 - Figure 3 As shown, a microfluidic chip for initial screening of blood donation is used in the field of initial screening of blood donors; it includes a chip body 1, which is a fan-shaped structure and is made of PMMA material and is manufactured using a mold injection molding process, wherein the fan-shaped angle can be adjusted according to the needs of the detection project, so that multiple samples can be detected on the machine at one time.

[0036] The chip body 1 is provided with a liquid capsule placement area 2, a diluent constant volume area 3, a sample one and diluent mixing area 4, a sample filling area 5, a sample constant volume and separation area 6, a sample two constant volume area 7 and an infectious disease reaction area 8; the liquid capsule placement area 2 is located at one end of the chip body 1, the diluent constant volume area 3 and the sample filling area 5 are arranged on one side of the liquid capsule placement area 2, the sample one and diluent mixing area 4 is located on the other side of the diluent constant volume area 3, the sample constant volume and separation area 6 and the sample two constant volume area 7 are both arranged on one side of the sample filling area 5, and the sample constant volume and separation area 6 is adjacent to the sample two constant volume area 7, a waste liquid pool 1 16 is provided on one side of the sample two constant volume area 7, and an infectious disease reaction area 8 is provided on one side of the waste liquid pool 16; a waste liquid pool 2 17 and a plurality of sample one reaction holes 9 are provided on one side of the sample one and diluent mixing area 4, the liquid capsule A waste liquid pool three 18 is correspondingly arranged on one side of the placement area 2 and the diluent constant volume area 3, and a sample two reaction hole 10 and two blood type reaction holes 15 are arranged between the waste liquid pool two 17 and the infectious disease reaction area 8; a connecting pipe 19 is arranged between the waste liquid pool two 17 and the sample one and diluent mixing area 4, the connecting pipe 19 is connected to the diluent constant volume area 3 through the microchannel one 11, the connecting pipe 19 is connected to the sample constant volume and separation area 6 through the microchannel two 12, the connecting pipe 19 is connected to the sample one and diluent mixing area 4 through the microchannel three 13, and the connecting pipe 19 is connected to the sample two constant volume area 7 through the microchannel four 14; the number of sample one reaction holes 9 is three, and the three sample one reaction holes 9, the waste liquid pool two 17, the sample two reaction hole 10, the two blood type reaction holes 15 and the infectious disease reaction area 8 are all connected to the connecting pipe 19.

[0037] Working principle:

[0038] A liquid capsule (containing 200ul of diluent) is pre-placed in liquid capsule placement area 2.

[0039] Reagents are pre-placed in the chip reaction wells (sample one reaction well 9, sample two reaction well 10 and blood type reaction well 15). The reagents in the whole blood area include: ABO blood type positive typing reagent, hemoglobin detection reagent, etc. The corresponding reagents in the infectious disease area include: hepatitis B surface antigen / syphilis treponema pallidum antibody combined detection reagent (gold label method), etc.; the corresponding reagents in the biochemical area include: total alanine aminotransferase detection reagent, transferrin detection reagent, etc.

[0040] Add sample (not less than 60ul) to sample filling area 5.

[0041] Place the chip into the instrument and let the instrument puncture the liquid capsule.

[0042] By centrifugation, the diluent is centrifuged to the diluent constant volume area 3; at the same time, the sample is centrifuged to the sample constant volume and separation area 6 and the sample second constant volume area 7, and the plasma and red blood cells in the sample constant volume and separation area 6 will be separated after centrifugation.

[0043] The chip stops centrifugation and pauses for a few seconds, at which time the liquid in the chip microchannels (ie, microchannel 1 11, microchannel 2 12, and microchannel 4 14) fills the entire microchannels under capillary action.

[0044] When the microchannel is filled with liquid, centrifugation continues. At this time, the diluent in the diluent constant volume area 3 and the plasma separated in the sample constant volume and separation area 6 will be centrifuged to the sample one and diluent mixing area 4 for mixing. At the same time, the sample in the sample two constant volume area 7 will be centrifuged to the sample two reaction hole 9, the blood type reaction hole 15, and the infectious disease reaction area 8 to react with the preset reagents.

[0045] When the sample 1 is fully mixed with the liquid in the diluent mixing zone 4 , the chip stops centrifugation and pauses for a few seconds to allow the microchannel 3 13 to be filled with liquid.

[0046] When the microchannel 3 is filled with liquid, centrifugation continues. At this time, the liquid in the "sample 1 dilution liquid mixing area" will be distributed to the sample 1 reaction well 9 and the sample 2 reaction well 10 to react with the preset reagents. At the same time, the instrument collects reaction data and finally obtains the test results.

[0047] Finally, the excess liquid in each step is discharged into the corresponding waste liquid pool.

[0048] The present invention mainly solves the problems of inaccurate results of the initial screening of blood donors, complicated operation, multiple sample additions, large blood consumption and high cost. Through the unique design of the internal structure, a series of operations such as sample addition, measurement, mixing, reaction and detection are integrated into the chip. The biochemical items, infectious disease items and blood type identification of the initial screening of blood donation are integrated into the chip. When in use, the chip can be tested by putting it into the machine after one sample addition. It has the characteristics of simple process, simple operation, high precision, full items and no need for professional inspectors to operate. With a small and low-cost micro-detection instrument, the integration and miniaturization of the detection system are realized. The chip adopts a fan-shaped structure, and the fan-shaped angle can be adjusted according to the needs of the detection project, so that multiple samples can be detected on the machine at one time.

[0049] Embodiment 2:

[0050] The bottom end surface of the chip body 1 is provided with a QR code area 20. The blood donor logs into the cloud platform to register by scanning the QR code in the QR code area 20 through the smart terminal. The cloud platform obtains the authorization of the blood donor and collects the personnel information of the authorized personnel, wherein the personnel information includes dietary data and work and rest data; the dietary data includes the food names and dietary times within a preset time period; the work and rest data includes several rest time periods and age within a preset time period; the personnel information is processed to obtain the initial screening value and is stored; specifically:

[0051] Processing the dietary data: matching the food name with a number of preset names, where each name corresponds to a preset food shadow value, summing up all the food shadow values ​​that match the food name in the dietary data to obtain the total food shadow value; sorting the dietary times in chronological order, calculating the duration between two adjacent dietary times to obtain the dietary interval duration, determining whether the food names corresponding to the two dietary times match the food shadow value, if both match the food shadow value, summing up the values ​​of the two food shadow values ​​and dividing them by the value corresponding to the dietary interval duration to obtain the dietary screening base value , sum up all the basic values ​​of food screening to get the total value of food screening, normalize the total value of food shadow and the total value of food screening and take the value between them, set the weight coefficients corresponding to the total value of food shadow and the total value of food screening, multiply the total value of food shadow and the total value of food screening by the corresponding weight coefficients respectively, and then sum them up to get the blood donor's food anomaly value; when the food anomaly value is greater than the set food anomaly threshold, a non-compliant blood donation reminder label is generated and sent to the smart terminal of the blood donor, and the association with the scanned QR code is removed; when the food anomaly value is less than or equal to the food anomaly threshold, the work and rest data is processed, and the specific process is as follows:

[0052] The rest time period is parsed to obtain the initial rest time, the end rest time and the rest duration, and the preset rest duration threshold corresponding to the age is obtained. The parsed rest duration is compared with the rest duration threshold. If the rest duration is less than the rest duration threshold, the difference between the two is calculated to obtain the low threshold duration, and all the values ​​of the low threshold duration are summed to obtain the total rest threshold value. The initial rest time and the end rest time are matched with several influencing time periods respectively, where each influencing time period corresponds to an end-of-life value; all the matched end-of-life values ​​are summed to obtain the total end-of-life value; the number of rest durations corresponding to the rest duration less than the rest duration threshold is counted to obtain the low threshold number, the low threshold number, the total rest threshold value and the total end-of-life value are normalized and the values ​​of the three are taken, and substituted into the end-of-life model to output the end-of-life value of the blood donor, where the end-of-life model is:

[0053] dyi, xyi, ayi are the values ​​corresponding to the number of low thresholds, the total value of rest thresholds and the total value of shadows, respectively; i represents the number of blood donors, XYi is the outlier value, μ1, μ2 and μ3 are the weight factors corresponding to the number of low thresholds, the total value of rest thresholds and the total value of shadows, respectively; their sizes are custom settings and are pre-stored in the cloud platform;

[0054] When the outlier value is greater than or equal to the set outlier threshold, a non-compliant blood donation reminder label is generated and sent to the smart terminal of the blood donor, and the association with the scanned QR code is canceled; when the outlier value is less than the set outlier threshold, the food outlier value and the outlier value are normalized and the value between the two is taken, and the values ​​of the two are converted into straight line lengths according to a certain ratio, and a circle is constructed with the straight line length of the food outlier value as the radius, and the straight line length of the circle is used as the height of the circle, and then a cone is constructed with the circle and the height, the volume of the cylinder is calculated, and the value of the volume is taken, and the preset fixed value is divided by the value of the volume to obtain the initial screening value of the blood donor.

[0055] The instrument recognizes the QR code in the QR code area 20 to obtain the initial screening value of the corresponding blood donor and displays it. If the initial screening value is greater than the set initial screening threshold, the chip body 1 is associated with the blood donor, and the test results are displayed and stored in the cloud platform; if the initial screening value or the set initial screening threshold, an early warning prompt is issued.

[0056] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A microfluidic chip for primary screening of blood donation, comprising a chip body (1), characterized in that: The chip body (1) is a fan-shaped structure, and is provided with a liquid capsule placement area (2), a diluent constant volume area (3), a sample 1 and diluent mixing area (4), a sample filling area (5), a sample constant volume and separation area (6), a sample 2 constant volume area (7) and an infectious disease reaction area (8); the liquid capsule placement area (2) is located at one end of the chip body (1), the diluent constant volume area (3) and the sample filling area (5) are arranged on one side of the liquid capsule placement area (2), the sample 1 and diluent mixing area (4) is located on the other side of the diluent constant volume area (3), the sample constant volume and separation area (6) and the sample 2 constant volume area (7) are both arranged on one side of the sample filling area (5), and the sample constant volume and separation area (6) is adjacent to the sample 2 constant volume area (7), a waste liquid pool 1 (16) is provided on one side of the sample 2 constant volume area (7), and an infectious disease reaction area (8) is provided on one side of the waste liquid pool 1 (16).

2. A microfluidic chip for primary screening of blood donation according to claim 1, characterized in that: A waste liquid pool 2 (17) and a plurality of sample 1 reaction holes (9) are arranged on one side of the sample 1 and diluent mixing area (4); a waste liquid pool 3 (18) is arranged correspondingly on one side of the liquid capsule placement area (2) and the diluent constant volume area (3); and a sample 2 reaction hole (10) and two blood type reaction holes (15) are arranged between the waste liquid pool 2 (17) and the infectious disease reaction area (8).

3. A microfluidic chip for primary screening of blood donation according to claim 2, characterized in that: A connecting pipe (19) is provided between the waste liquid pool 2 (17) and the sample 1 and diluent mixing area (4); the connecting pipe (19) is connected to the diluent constant volume area (3) through the microfluidic channel 1 (11); the connecting pipe (19) is connected to the sample constant volume and separation area (6) through the microfluidic channel 2 (12); the connecting pipe (19) is connected to the sample 1 and diluent mixing area (4) through the microfluidic channel 3 (13); and the connecting pipe (19) is connected to the sample 2 constant volume area (7) through the microfluidic channel 4 (14).

4. A microfluidic chip for primary screening of blood donation according to claim 3, characterized in that: The number of the sample one reaction holes (9) is three, and the three sample one reaction holes (9), the waste liquid pool two (17), the sample two reaction hole (10), the two blood type reaction holes (15) and the infectious disease reaction area (8) are all connected to the connecting pipe (19).

5. A microfluidic chip for primary screening of blood donation according to claim 4, characterized in that: The specific working method of the chip includes the following steps: Step 1: pre-placing a liquid capsule in the liquid capsule placement area (2); Step 2: pre-place reagents in the sample 1 reaction well (9), the sample 2 reaction well (10) and the blood type reaction well (15); Step 3: Add sample into the sample filling area (5); Step 4: Place the chip body (1) into the instrument, and use the instrument to puncture the liquid capsule; Step 5: centrifuge the diluent to the diluent constant volume area (3), and centrifuge the sample to the sample constant volume and separation area (6) and the sample second constant volume area (7), and the plasma and red blood cells in the sample constant volume and separation area (6) will be separated after centrifugation; Step 6: Stop the centrifugation and pause for a few seconds. At this time, the liquids in microchannel 1 (11), microchannel 2 (12) and microchannel 4 (14) will fill the entire microchannel due to capillary action; Step 7: When the microchannel is filled with liquid, centrifugation continues. At this time, the diluent in the diluent constant volume area (3) and the plasma separated in the sample constant volume and separation area (6) are centrifuged to the sample 1 and diluent mixing area (4) for mixing. At the same time, the sample in the sample 2 constant volume area (7) is centrifuged to the sample 2 reaction hole (10), the blood type reaction hole (15) and the infectious disease reaction area (8) to react with the preset reagents. Step 8: When the sample 1 is fully mixed with the liquid in the diluent mixing zone (4), the centrifugation is stopped and paused for a few seconds to allow the microchannel 3 (13) to be filled with liquid; Step 9: When the microchannel 3 (13) is filled with liquid, centrifugation is continued. At this time, the liquid in the sample 1 and diluent mixing area (4) will be distributed to the sample 1 reaction well (9) and the sample 2 reaction well (10) to react with the preset reagent. At the same time, the instrument collects reaction data and finally obtains the test result; Step 10: Drain the excess liquid from each step into the corresponding waste liquid pool 1 (16), waste liquid pool 2 (17) and waste liquid pool 3 (18).

6. A microfluidic chip for primary screening of blood donation according to claim 5, characterized in that: The bottom end surface of the chip body (1) is provided with a QR code area (20). The blood donor logs into the cloud platform to register by scanning the QR code in the QR code area (20) through a smart terminal. The cloud platform obtains the blood donor's authorization and collects the authorized person's personal information, wherein the personal information includes dietary data and work and rest data; the dietary data includes the names of foods and dietary times within a preset time period; the work and rest data includes a number of rest time periods and age within a preset time period; the personal information is processed to obtain a preliminary screening value and is stored; the instrument recognizes the QR code in the QR code area (20) to obtain the preliminary screening value of the corresponding blood donor and displays it; if the preliminary screening value is greater than a set preliminary screening threshold, the chip body (1) is associated with the blood donor, and the test result is displayed and stored in the cloud platform; if the preliminary screening value or the set preliminary screening threshold is exceeded, an early warning prompt is issued.

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