Germination accelerating treatment method for celery seeds

Abstract

The invention belongs to the field of vegetables, and provides a celery seed germination accelerating treatment method and a seedling raising method, and the celery seed germination accelerating treatment method comprises the operation of treating celery seeds by adopting a nano material. The celery seed germination accelerating treatment method and the celery seedling raising method provided by the invention are particularly suitable for celery germination accelerating and seedling raising under a high-temperature condition, by adding a nano material, the germination rate of the celery seeds' Beijing-celery No. 2 'and' Beijing-celery No. 3 'is obviously improved, and meanwhile, the germination speed, the seed vigor and the seedling vigor of the celery seeds can also be improved; the excessive growth of seedlings is effectively inhibited, the seedling raising period is shortened, the yield increase is promoted, the seedlings with higher quality are produced more quickly, the stress resistance of the seedlings is improved, and the seedlings are easier to survive after being planted; and the cost is low, the effect is good, popularization and application are facilitated, and economic benefits are obviously improved.

Description

Technical Field

[0001] The invention belongs to the technical field of vegetables, and particularly relates to a celery seed germination processing and seedling raising method under high temperature conditions. Background Art

[0002] Celery (Apium graveolens L.), also known as dry celery and foreign celery, is an annual or biennial vegetable of the Umbelliferae family. It is native to the Mediterranean coast and is rich in vitamins, minerals and volatile aromatic oils. It has a spicy taste and can lower blood pressure and strengthen the brain. Celery seeds are small and double-hanging fruits. They are often hardened by the leathery outer skin and have oil glands, which makes them difficult to penetrate water. They germinate very slowly and unevenly. Celery is a cool vegetable and has good heat resistance in the seedling stage, but when the temperature exceeds 25°C, seed germination will be inhibited. Raising seedlings in summer is more difficult, and the germination rate is generally only 50% to 60%, which affects field seedling formation and plant yield.

[0003] Nanomaterials refer to materials that have at least one dimension in the nanoscale range (1-100nm) in three-dimensional space or are composed of them as basic units. Nanomaterials are widely used in agriculture and can be used as pesticides, fertilizers, veterinary drugs, etc. In addition, they also have a positive effect on plant growth regulation. However, few research reports have shown whether nanomaterials have a promoting effect on celery seed germination under adverse conditions. Summary of the invention

[0004] In order to solve the above problems existing in the prior art, the first aspect of the present invention provides a method for accelerating the germination of celery seeds, which comprises the operation of treating the celery seeds with nanomaterials.

[0005] A second aspect of the present invention provides a celery seedling raising method, which comprises the operation of adopting the celery seed germination treatment method provided by the first aspect of the present invention.

[0006] The celery seed germination treatment method and celery seedling raising method provided by the present invention are particularly suitable for celery germination and seedling raising under high temperature conditions. The present invention significantly improves the germination rate of 'Jingqin No. 2' and 'Jingqin No. 3' celery seeds by adding nanomaterials, and can also improve the germination speed, seed vitality, and seedling vitality of the two celery seeds, effectively inhibit the excessive growth of seedlings, shorten the seedling raising period and promote yield increase, produce seedlings of higher quality faster, improve the ability of seedlings to resist stress, and make seedlings more likely to survive after planting; and the present invention has low cost and good effect, is conducive to promotion and use, and significantly improves economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] Figure 1 The effects of six different treatments on the germination rate (%) of 'Jingqin No. 2' celery seeds are shown (n=3), and the error bars represent standard errors.

[0008] Figure 2 The effects of six different treatments on the germination rate (%) of 'Jingqin No. 3' celery seeds are shown (n=3), and the error bars represent standard errors.

[0009] Figure 3 The effects of six different treatments on the germination potential of 'Jingqin No. 2' and 'Jingqin No. 3' are shown. Error bars represent standard errors, and letters represent significant differences under Tukey's test (P=0.05).

[0010] Figure 4 The effects of six different treatments on the germination index of 'Jingqin No. 2' and 'Jingqin No. 3' are shown. Error bars represent standard errors, and letters represent significant differences under Tukey's test (P=0.05).

[0011] Figure 5 The effects of six different treatments on the seedling vigor index of 'Jingqin No. 2' and 'Jingqin No. 3' are shown. Error bars represent standard errors, and letters represent significant differences under Tukey's test (P=0.05).

[0012] Figure 6 The effects of six different treatments on the seedling index of 'Jingqin No. 2' and 'Jingqin No. 3' are shown. Error bars represent standard errors, and letters represent significant differences under Tukey's test (P=0.05).

[0013] Figure 7 The effects of six different treatments on the stress resistance index of seedling length of 'Jingqin No. 2' and 'Jingqin No. 3' are shown. Error bars represent standard errors, and letters represent significant differences under Tukey's test (P=0.05).

[0014] Figure 8 The effects of six different treatments on the root length stress resistance index of 'Jingqin No. 2' and 'Jingqin No. 3' are shown. Error bars represent standard errors, and letters represent significant differences under Tukey's test (P=0.05).

[0015] Fig. 9 The effects of six different treatments on the dry matter distribution rate (%) of 'Jingqin No. 2' are shown (n=15), error bars represent standard errors, and different letters represent significant differences under Tukey's test (P=0.05).

[0016] Fig.10 The effects of six different treatments on the dry matter distribution rate (%) of 'Jingqin No. 3' are shown (n=15), error bars represent standard errors, and different letters represent significant differences under Tukey's test (P=0.05).

[0017] Fig.11The effect of six different nanomaterials on the growth of 'Jingqin No. 2' celery is shown. From left to right, the plants are multi-walled carbon nanotubes (C:MWCNTs), nano silicon (Si:Si-NPs), nano magnesium oxide (MgO:MgO-NPs), nano zinc oxide (ZnO:ZnO-NPs), L-aspartate nano calcium (Ca:Ca(L-asp)-NPs) and celery seedlings under the control treatment (CK). Celery seeds were sown on July 25, 2024, and the photos were taken on September 25, 2024. The plant size in the figure (including the size of the whole plant including the roots and the aboveground parts) reflects the growth of the plant. The shorter the hypocotyl (the part between the cotyledons and the roots) or the larger the plant, the more vigorous the seedling growth and the higher the seedling quality.

[0018] Fig.12 Shows the effects of six different nanomaterials on the growth of 'Jingqin No. 3' celery (scale bar and Fig.11 The plants from left to right are multi-walled carbon nanotubes (C:MWCNTs), nano-silicon (Si:Si-NPs), nano-magnesium oxide (MgO:MgO-NPs), nano-zinc oxide (ZnO:ZnO-NPs), L-aspartate nano-calcium (Ca:Ca(L-asp)-NPs) and celery seedlings under the control treatment (CK). Celery seeds were sown on July 25, 2024, and the photos were taken on September 25, 2024. The plant size in the figure (including the size of the entire plant including the roots and the aboveground parts) reflects the growth of the plant. The shorter the hypocotyl or the larger the plant, the more vigorous the seedling growth and the higher the seedling quality.

[0019] Figure 13-18 The results of six different treatments on the germination of 'Jingqin No. 2' celery seeds are shown in the figure. Figures 13 to 18 They are treated with multi-walled carbon nanotubes, nano-silicon, nano-magnesium oxide, nano-zinc oxide, L-aspartic acid nano-calcium, and control CK. Celery seeds were sown on July 25, 2024, and the photos were taken on August 16, 2024. In the picture, the green part is the celery seedlings after germination, and the amount of green area (reflecting the number of celery seedlings) reflects the number of germinated celery seeds. The more green area, the more celery seeds germinated, and the higher the germination rate.

[0020] Figure 19-24 The results of six different treatments on the germination of 'Jingqin No. 3' celery seeds are shown in the figure. Figures 13 to 18The treatments were multi-walled carbon nanotubes, nano-silicon, nano-magnesium oxide, nano-zinc oxide, L-aspartic acid nano-calcium, and control CK. Celery seeds were sown on July 25, 2024, and the photos were taken on August 16, 2024. In the picture, the green part is the celery seedlings after germination, and the amount of green area reflects the number of celery seeds that germinated. The more green area, the more celery seeds germinated, and the higher the germination rate. DETAILED DESCRIPTION

[0021] To make the technical solution, purpose and advantages of the present invention clearer, the present invention is further described in detail below through specific implementation examples. It should be understood that the specific implementation methods described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0022] A first aspect of the present invention provides a method for accelerating germination of celery seeds, the method comprising the operation of treating the celery seeds with nanomaterials.

[0023] According to the first aspect of the present invention, the nanomaterial may be a nanomaterial with a molecular weight between 12-45.

[0024] Preferably, the nanomaterial is at least one of multi-walled carbon nanotubes, nano-silicon and nano-magnesium oxide.

[0025] Furthermore, the molecular weight of the multi-walled carbon nanotube is 12.11, the diameter is 30-80 nm, and the length is 0.5-2 μm.

[0026] The molecular weight of the nano-silicon is 28.09 and the diameter is 20-60 nm.

[0027] The molecular weight of the nano magnesium oxide is 40.3, and the diameter is 50-100 nm.

[0028] According to the first aspect of the present invention, the method is more suitable for germination treatment of celery seeds under high temperature conditions (25° C. and above, such as high temperature conditions in summer).

[0029] According to the first aspect of the present invention, the celery is 'Jingqin No. 2' and 'Jingqin No. 3'.

[0030] Among them, for 'Jingqin No. 2', it is preferred to use multi-walled carbon nanotubes and / or nano magnesium oxide for treatment.

[0031] Among them, for Jingqin No. 3', it is preferred to use multi-walled carbon nanotubes and / or nano-silicon for treatment.

[0032] According to the first aspect of the present invention, the operation of treating celery seeds with nanomaterials includes: uniformly mixing an aqueous solution containing nanomaterials with a matrix to obtain a matrix containing nanomaterials, and then planting celery seeds in the matrix containing nanomaterials.

[0033] Wherein, the concentration of the nanomaterial can be 30-70 mg / L, for example, any one of 30, 35, 40, 45, 50, 55, 60, 65, 70 mg / L or a range between two thereof, for example, 45-55 mg / L, 40-60 mg / L, 30-55 mg / L, 35-50 mg / L, 45-65 mg / L, 50-70 mg / L, preferably 50 mg / L.

[0034] The dosage of the nanomaterial can be: 0.2-0.7 kg (preferably 0.5 kg) of matrix is ​​mixed with every 1 mg of nanomaterial.

[0035] A second aspect of the present invention provides a celery seedling raising method, which comprises the operation of adopting the celery seed germination treatment method provided by the first aspect of the present invention.

[0036] According to the second aspect of the present invention, the method is more suitable for growing celery seedlings under high temperature conditions (25° C. and above, such as high temperature conditions in summer).

[0037] The various reagents, materials, etc. used in the following examples, unless otherwise specified, are all products that can be obtained from commercial channels; the various tests and detection methods used in the following examples, unless otherwise specified, are all conventional tests and detection methods in the art and can be obtained from textbooks, reference books or academic journals.

[0038] In the following embodiments:

[0039] Plant materials and growth environment

[0040] To determine the effects of different nanomaterials on celery seed germination under high temperature conditions, experiments were conducted in a glass greenhouse at the Vegetable Research Institute of Beijing Academy of Agricultural and Forestry Sciences (39.96N, 116.26E) from July 25, 2024 to September 25, 2024. Two room-temperature-stored celery seeds (Apium graveolens L. 'Jingqin No. 2') and (Apium graveolens L. 'Jingqin No. 3') (Beijing Yanyinong (Beijing) Seed Technology Co., Ltd., Beijing) were directly sown on 128-hole seedling trays filled with seedling medium (Beijing Quanshun Flower Soil Factory, Beijing), with 100 seeds sown in each treatment for three times. After sowing, a thin layer of vermiculite (Beijing Quanshun Flower Soil Factory, Beijing) was covered to maintain the moisture of the medium. Tap water was used to water once in the morning, noon, and evening each day, with 300 mL of water per tray each time, and the air temperature was always kept above 25°C. It was observed that the two types of celery seeds germinated successively from August 1, 2024. The statistics of germination rate started from August 1 to August 16. When the celery seedlings reached the commercial seedling standard (i.e., 5 true leaves, two months after sowing), samples were collected for measurement of growth parameters.

[0041] Experimental treatment

[0042] The treatment started on July 25, 2024 and ended on September 25, for a total of 2 months. Five different nanomaterials: multi-walled carbon nanotubes (C: MWCNTs), nano silicon (Si: Si-NPs), nano magnesium oxide (MgO: MgO-NPs), nano zinc oxide (ZnO: ZnO-NPs) (Aladdin Biochemical Technology Co., Ltd., Shanghai), L-aspartate nano calcium (Ca: Ca (L-asp)-NPs) (Sanya Baitai Biotechnology Co., Ltd., Hainan) were dissolved in 200 mL of pure water, and an ultrasonic cleaner (KQ5200DE, Kunshan Ultrasonic Instrument Co., Ltd., Jiangsu) was used to shake 3 times at room temperature at 100% power for 20 minutes each time to prepare a nano solution with a concentration of 50 mg / L. 200 mL of the nano solution was mixed evenly with 5 kg of the seedling matrix, and loaded into a 128-hole seedling tray. The experimental control (CK) was set to no nanomaterials added. The information of the nanomaterials used in the experiment is shown in Table 1.

[0043] Table 1 Nanomaterial information

[0044] Processing Name Molecular formula Molecular weight Diameter(nm) Length(μm) Multi-walled carbon nanotubes C 12.11 30-80 0.5-2 Nano Silicon Si 28.09 20-60 - Nano magnesium oxide MgO 40.3 50-100 - Nano zinc oxide ZnO 81.39 50±10 - L-Aspartic Acid Nano Calcium Ca(L-asp) 304.2 2-80 -

[0045] Example 1

[0046] This example is used to illustrate the effects of five different nanomaterials on the germination rates of 'Jingqin No. 2' and 'Jingqin No. 3'.

[0047] Germination rate (%) = G X / NX×100%, where G X represents the number of germinations at X days, and NX represents the total number of seeds.

[0048] like Figure 1 The figure shows the effects of six different treatments on the germination rate of 'Jingqin No. 2'. Compared with the control, multi-walled carbon nanotubes and nano-magnesium oxide promoted the germination of 'Jingqin No. 2' and increased the germination rate of 'Jingqin No. 2', with similar effects; while nano-silicon, nano-zinc oxide, and L-aspartic acid nano-calcium reduced the germination rate of 'Jingqin No. 2' and had an inhibitory effect on the germination of 'Jingqin No. 2'.

[0049] Depend on Figure 13-18 It can be seen that compared with nano-silicon, nano-zinc oxide, L-aspartic acid nano-calcium treatment and the control CK, the 'Jingqin No. 2' treated with multi-walled carbon nanotubes and nano-magnesium oxide has more celery seedlings. It can be seen that the multi-walled carbon nanotubes and nano-magnesium oxide treatment increased the germination rate of 'Jingqin No. 2'.

[0050] like Figure 2 The figure shows the effect of 6 different treatments on the germination rate of 'Jingqin No. 3'. Compared with the control, multi-walled carbon nanotubes and nano-silicon promoted the germination of 'Jingqin No. 3' and increased the germination rate of 'Jingqin No. 3'; and the effect of nano-silicon was slightly better than that of carbon nanotubes 8-19 days after sowing, and the effect was similar after the 19th day of sowing. Nano-magnesium oxide promoted the germination of 'Jingqin No. 3' before the 20th day after sowing, but the effect was not as good as carbon nanotubes and nano-silicon; but 20 days after sowing and thereafter, the effect on the germination rate of 'Jingqin No. 3' was not as good as the control (CK). In addition, nano-zinc oxide and nano-calcium L-aspartate reduced the germination rate of 'Jingqin No. 3' and had an inhibitory effect on the germination of 'Jingqin No. 3'.

[0051] Depend on Figure 19-24 It can be seen that compared with nano-magnesium oxide, nano-zinc oxide, L-aspartic acid nano-calcium treatment and control CK, the 'Jingqin No. 3' treated with multi-walled carbon nanotubes and nano-silicon has more celery seedlings, which shows that the multi-walled carbon nanotubes and nano-silicon treatment increased the germination rate of 'Jingqin No. 3'.

[0052] In summary, it can be seen that the multi-walled carbon nanotubes have a significant promoting effect on the germination of "Jingqin No. 2" and "Jingqin No. 3", and improve the germination rate of "Jingqin No. 2" and "Jingqin No. 3". Moreover, the response of different varieties of celery to nanomaterials during germination is not exactly the same.

[0053] Example 2

[0054] This example is used to illustrate the effects of five different nanomaterials on seedling parameters of 'Jingqin No. 2' and 'Jingqin No. 3' (including germination potential, germination index, seedling vitality index, seedling growth index, seedling length stress resistance index, and root length stress resistance index).

[0055] Germination potential (% / 10 days) = G10 / NX×100%.

[0056] Germination index (% / 22 days) = ∑G X / D X .

[0057] Among them, G10 is the number of seeds germinated on the 10th day of seed germination, NX is the total number of seeds, and G X represents the number of germinations at X days, D X The corresponding germination time.

[0058] Seedling vigor index = (average root length (cm) + average stem length (cm)) × average germination rate.

[0059] Seedling index = (stem diameter (mm) / plant height (root to leaf, cm) + underground dry weight (g) / aboveground dry weight (g)) × whole plant dry weight (g).

[0060] Seedling length stress resistance index (%) = average seedling length of treated seedlings (cm) / average seedling length of control seedlings (cm) × 100%.

[0061] Root length stress resistance index (%) = average root length of treated seedlings (cm) / average root length of control seedlings (cm) × 100%.

[0062] like Figure 3 As shown, the effects of 6 different treatments on the germination potential of 'Jingqin No. 2' and 'Jingqin No. 3'. For 'Jingqin No. 2': multi-walled carbon nanotubes and nano-magnesium oxide treatments improved the germination potential of 'Jingqin No. 2' and the germination rate of seeds of 'Jingqin No. 2'; however, nano-silicon, nano-zinc oxide, and L-aspartic acid nano-calcium treatments had a counter-effect on the germination potential of 'Jingqin No. 2', reducing the germination rate of seeds of 'Jingqin No. 2'. For 'Jingqin No. 3': multi-walled carbon nanotubes and nano-silicon treatments significantly improved the germination potential of 'Jingqin No. 3', indicating that these two treatments improved the germination rate of seeds of 'Jingqin No. 3'; however, nano-magnesium oxide treatment did not significantly improve the germination potential of 'Jingqin No. 3', and nano-zinc oxide and L-aspartic acid nano-calcium treatments had a counter-effect on the germination potential of 'Jingqin No. 3', reducing the germination rate of seeds of 'Jingqin No. 3'.

[0063] like Figure 4As shown, the effects of 6 different treatments on the germination index of 'Jingqin No. 2' and 'Jingqin No. 3'. For 'Jingqin No. 2': the treatments of multi-walled carbon nanotubes and nano-magnesium oxide increased the germination index of 'Jingqin No. 2', indicating that these two treatments increased the seed vitality of 'Jingqin No. 2'; however, the treatments of nano-silicon, nano-zinc oxide, and L-aspartic acid nano-calcium had a counter-effect on the germination index of 'Jingqin No. 2', reducing the seed vitality of 'Jingqin No. 2'. For 'Jingqin No. 3': the treatments of multi-walled carbon nanotubes and nano-silicon significantly increased the germination index of 'Jingqin No. 3', indicating that these two treatments increased the seed vitality of 'Jingqin No. 3'; however, the treatment of nano-magnesium oxide did not significantly increase the germination index of 'Jingqin No. 3', and the treatments of nano-zinc oxide and L-aspartic acid nano-calcium had a counter-effect on the germination index of 'Jingqin No. 3', reducing the seed vitality of 'Jingqin No. 3'.

[0064] like Figure 5 As shown, the effects of 6 different treatments on the seedling vigor index of 'Jingqin No. 2' and 'Jingqin No. 3'. For 'Jingqin No. 2': the treatments of multi-walled carbon nanotubes and nano-magnesium oxide increased the seedling vigor index of 'Jingqin No. 2', that is, the potential activity and performance level of 'Jingqin No. 2' seeds during germination and seedling emergence; however, the treatments of nano-silicon, nano-zinc oxide, and L-aspartic acid nano-calcium had a counter-effect on the seedling vigor index of 'Jingqin No. 2'. For 'Jingqin No. 3': the treatments of multi-walled carbon nanotubes and nano-silicon increased the seedling vigor index of 'Jingqin No. 3', that is, the potential activity and performance level of 'Jingqin No. 3' seeds during germination and seedling emergence; the treatment of nano-magnesium oxide also increased the seedling vigor index of 'Jingqin No. 3', but the increase was not as great as that of the two treatments of multi-walled carbon nanotubes and nano-silicon. The treatments of nano-zinc oxide and L-aspartic acid nano-calcium had basically no effect on the seedling vigor index of 'Jingqin No. 3'.

[0065] like Figure 6 As shown, the effects of 6 different treatments on the seedling index of 'Jingqin No. 2' and 'Jingqin No. 3'. For 'Jingqin No. 2': Compared with multi-walled carbon nanotubes, nano zinc oxide and L-aspartic acid nano calcium, nano magnesium oxide treatment significantly increased the seedling index of 'Jingqin No. 2', indicating that the seedling quality of nano magnesium oxide treatment is better than that of multi-walled carbon nanotubes, nano zinc oxide and L-aspartic acid nano calcium, but the effect is not obvious compared with nano silicon and control treatment. For 'Jingqin No. 3': All 5 different treatments except CK have no obvious effect on the seedling index of 'Jingqin No. 3', that is, all treatments have no obvious effect on improving the quality of seedlings. The higher seedling index of 'Jingqin No. 3' than 'Jingqin No. 2' may be because 'Jingqin No. 3' is a variety with vigorous growth and fast growth, and can produce higher quality seedlings faster in the same time.

[0066] like Figure 7As shown, the effects of 6 different treatments on the seedling length stress resistance index of 'Jingqin No. 2' and 'Jingqin No. 3'. For 'Jingqin No. 2': Compared with the control, the nano-magnesium oxide treatment improved the seedling length stress resistance index of 'Jingqin No. 2', while the three treatments of multi-walled carbon nanotubes, nano-silicon, and nano-zinc oxide had no significant effect on the seedling length stress resistance index of 'Jingqin No. 2', and L-aspartic acid nano-calcium significantly reduced the seedling length stress resistance index of 'Jingqin No. 2'. For 'Jingqin No. 3': All 5 different treatments except CK improved the seedling length stress resistance index of 'Jingqin No. 3', among which the nano-magnesium oxide treatment improved the seedling length stress resistance index of 'Jingqin No. 3' the most significantly. The improvement of the seedling length stress resistance index can improve the ability of seedlings to resist stress, which may make it easier for seedlings to survive after planting.

[0067] like Figure 8 The figure shows the effects of 6 different treatments on the root length stress resistance index of 'Jingqin No. 2' and 'Jingqin No. 3'. It can be seen that for 'Jingqin No. 2' and 'Jingqin No. 3', all 5 different treatments had no significant effect on the root length stress resistance index of 'Jingqin No. 2' and 'Jingqin No. 3'.

[0068] In summary, the treatment of multi-walled carbon nanotubes and nano-magnesium oxide can improve the germination potential, germination index, and seedling vigor index of "Jingqin No. 2". Therefore, it can be seen that the treatment of multi-walled carbon nanotubes and nano-magnesium oxide can promote the germination of "Jingqin No. 2". The treatment of multi-walled carbon nanotubes and nano-silicon can improve the germination potential, germination index, seedling vigor index, and seedling length resistance index of "Jingqin No. 3". Therefore, it can be seen that the treatment of multi-walled carbon nanotubes and nano-silicon can promote the germination of "Jingqin No. 3".

[0069] Example 3

[0070] This example is used to illustrate the effects of five different nanomaterials on the growth parameters of 'Jingqin No. 2' and 'Jingqin No. 3' seedlings.

[0071] Root-shoot ratio (R:S) = underground dry weight (mg) / aboveground dry weight (mg).

[0072] Specific leaf area (SLA) = third true leaf area (mm 2 ) / dry weight of the third true leaf (mg).

[0073] Leaf dry matter content (LDMC) = leaf dry weight (mg) / leaf fresh weight (g).

[0074] The SPAD value indicates the relative content of chlorophyll in plant leaves and is the average value obtained by measuring three times using a chlorophyll meter (SPAD-502Plus, KONICA MINOLTA SENSING, INC., Japan).

[0075] The growth parameters of 'Jingqin No. 2' are shown in Table 2.

[0076] Table 2 Growth parameters of 'Jingqin No. 2'

[0077]

[0078] The growth parameters of 'Jingqin No. 3' are shown in Table 3.

[0079] Table 3 Growth parameters of 'Jingqin No. 3'

[0080]

[0081] In Tables 2 and 3, z is the mean ± standard error (n = 15), and y with different letters indicates significant differences in the data under Tukey's test (P = 0.05).

[0082] As shown in Tables 2 and 3, both the treatments of multi-walled carbon nanotubes and nano-silicon can shorten the hypocotyls of the celery seedlings of 'Jingqin No. 2' and 'Jingqin No. 3' compared with those of the celery seedlings treated with other treatments, effectively inhibiting the leggy growth of the seedlings. Under the treatment of nano-magnesium oxide, the aboveground fresh weight of the two celery species was the largest. Magnesium is usually related to chlorophyll synthesis, and chlorophyll is related to plant photosynthesis. Although the aboveground fresh weight of the two celery species did not increase significantly compared with the control under the treatment of nano-magnesium oxide, we speculate that the celery treated with nano-magnesium oxide may improve its photosynthesis capacity, thereby shortening the seedling period and increasing production.

[0083] Table 4 shows the dry weight of each part of 'Jingqin No. 2'.

[0084] Table 4 Dry weight of each part of 'Jingqin No. 2'

[0085]

[0086] Table 5 shows the dry weight of each part of 'Jingqin No. 3'.

[0087] Table 5 Dry weight of each part of 'Jingqin No. 3'

[0088]

[0089] In Tables 4 and 5, z is the mean ± standard error (n = 15), and y with different letters indicates significant differences in the data under Tukey's test (P = 0.05).

[0090] From Table 4 and Fig. 9 It can be seen that compared with the control CK, C, Si, ZnO and Ca treatments caused the dry matter of 'Jingqin No. 2' to tend to be distributed to the leaves, but the effect was not obvious. In addition, the dry matter distribution of the stems treated with C, Si and Ca was significantly reduced, and the 'Jingqin No. 2' treated with C, Si and Ca may have a tendency to grow into leaf celery.

[0091] From Table 5 and Fig.10 It can be seen that compared with the control CK, all five different treatments had no significant effect on the dry matter distribution of 'Jingqin No. 3'.

[0092] In addition, by Fig.11 It can be seen that the hypocotyls of celery seedlings treated with multi-walled carbon nanotubes and nano-silicon are significantly shorter than those treated with nano-zinc oxide, nano-calcium L-aspartate and control CK; it can be seen that multi-walled carbon nanotubes and nano-silicon treatment can effectively inhibit the leggy growth of seedlings, and the seedling quality is higher than that of nano-zinc oxide, nano-calcium L-aspartate and control CK. Compared with nano-zinc oxide, nano-calcium L-aspartate and control CK treatments, celery seedlings treated with nano-magnesium oxide have the largest plants and grow vigorously, and the quality of celery seedlings treated with nano-magnesium oxide is higher.

[0093] Depend on Fig.12 It can be seen that the hypocotyls of celery seedlings treated with multi-walled carbon nanotubes and nano-silicon are significantly shorter than those treated with nano-zinc oxide, nano-calcium L-aspartate and control CK; it can be seen that multi-walled carbon nanotubes and nano-silicon treatments effectively inhibit the elongation of seedlings, and the seedling quality is higher than that of nano-zinc oxide, nano-calcium L-aspartate and control CK. Compared with nano-zinc oxide, nano-calcium L-aspartate and control CK treatments, celery seedlings treated with nano-magnesium oxide have the largest plants and grow vigorously, and the quality of celery seedlings treated with nano-magnesium oxide is higher.

[0094] In summary, in Examples 1-3, the three nanomaterials with molecular weights between 12-45, namely, multi-walled carbon nanotubes, nano-silicon and nano-magnesium oxide, can increase the germination rate of celery by more than 20% during the high temperature period in summer, and effectively increase the commercial seedling rate, with an estimated maximum yield increase of 25%.

[0095] Solution 1 to solve the low celery germination rate in summer high temperature in actual production:

[0096] Multi-walled carbon nanotubes and nano-magnesium oxide can improve the seed germination rate of 'Jingqin No. 2' by enhancing the seed vitality under high temperature conditions. However, the treatment with multi-walled carbon nanotubes will sacrifice the fresh weight and dry weight of celery seedlings. If production focuses on yield, it is recommended to use nano-magnesium oxide.

[0097] Composite-walled carbon nanotubes and nano-silicon can improve the germination rate of 'Jingqin No. 3' seeds under high temperature conditions, significantly shorten the length of the hypocotyl of celery seedlings compared with the control, and have no significant effect on the dry and fresh weight of the seedlings. Considering the price (C: 98 yuan / g, Si: 91.9 yuan / g), nano-silicon is preferred.

[0098] Solution 2 to solve the low celery germination rate in summer high temperature in actual production:

[0099] Multi-walled carbon nanotubes can improve the germination rate of two types of celery, 'Jingqin No. 2' and 'Jingqin No. 3', under high temperature conditions. In actual production, there is no need to consider the variety. After being made into a product, it is easy to use and easy to promote.

[0100] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for accelerating germination of celery seeds, characterized in that: The method comprises the operation of treating celery seeds with nano materials.

2. The method for accelerating germination of celery seeds according to claim 1, characterized in that: The molecular weight of the nanomaterial is between 12-45.

3. The method for accelerating germination of celery seeds according to claim 2, characterized in that: The nano material is at least one of multi-walled carbon nanotubes, nano silicon and nano magnesium oxide.

4. The method for accelerating germination of celery seeds according to claim 3, characterized in that: The molecular weight of the multi-walled carbon nanotube is 12.11, the diameter is 30-80 nm, and the length is 0.5-2 μm.

5. The method for accelerating germination of celery seeds according to claim 3, characterized in that: The molecular weight of the nano-silicon is 28.09 and the diameter is 20-60 nm.

6. The method for accelerating germination of celery seeds according to claim 3, characterized in that: The molecular weight of the nano magnesium oxide is 40.3, and the diameter is 50-100 nm.

7. The method for accelerating germination of celery seeds according to any one of claims 1 to 6, characterized in that: The method is suitable for accelerating the germination of celery seeds under high temperature conditions of 25°C or above.

8. The method for accelerating germination of celery seeds according to claim 7, characterized in that: For 'Jingqin No. 2', multi-walled carbon nanotubes and / or nano-magnesium oxide are used for treatment; For 'Jingqin No. 3', multi-walled carbon nanotubes and / or nano-silicon are used for treatment.

9. The method for accelerating germination of celery seeds according to claim 7, characterized in that: The process of treating celery seeds with nanomaterials includes: uniformly mixing an aqueous solution containing nanomaterials with a matrix to obtain a matrix containing nanomaterials, and then planting celery seeds in the matrix containing nanomaterials; Preferably, the concentration of the nanomaterial is 30-70 mg / L, such as any one or two of 30, 35, 40, 45, 50, 55, 60, 65, 70 mg / L, such as 45-55 mg / L, 40-60 mg / L, 30-55 mg / L, 35-50 mg / L, 45-65 mg / L, 50-70 mg / L, preferably 50 mg / L; Preferably, the amount of the nanomaterial is: 0.2-0.7 kg, preferably 0.5 kg, of the matrix is ​​mixed with every 1 mg of the nanomaterial.

10. A method for raising celery seedlings, comprising the operation of adopting the method for accelerating germination of celery seeds according to any one of claims 1 to 9.

Images