Microbial agent for improving straw conversion efficiency and application thereof

Abstract

The invention discloses a microbial agent for improving straw conversion efficiency and application thereof, relates to the technical field of straw conversion, and aims to solve the problems that the application effects of many microbial agents in the market at present are uneven, and the conversion efficiency cannot reach the expectation due to low strain activity and poor adaptability of part of products. The microbial agent fuses the following microbial populations and additives: the microbial populations comprise screened saccharomyces cerevisiae, bacillus amyloliquefaciens and trichoderma viride, and the saccharomyces cerevisiae, the bacillus amyloliquefaciens and the trichoderma viride are loaded on the same carrier system. The viable bacteria ratio of the saccharomyces cerevisiae to the bacillus amyloliquefaciens to the trichoderma viride is 1: 1.8: 1.4; the carrier system is formed by mixing rice straw powder and bentonite according to the optimized mass ratio of 1: 1, and aims to provide an inhabiting and breeding environment for microorganisms. The method has the advantages that efficient biodegradation is realized, and the straw conversion efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of straw conversion, and more specifically to a microbial agent for improving straw conversion efficiency and application thereof. Background Art

[0002] In agricultural production, straw, as a major agricultural residue, has a huge annual output, but its processing and utilization efficiency is generally low. Traditionally, many farmers deal with straw by burning or randomly stacking it, which not only causes waste of resources, but also causes environmental pollution and fire risks. Therefore, how to convert straw efficiently and environmentally friendly has become a technical problem that needs to be urgently solved in current agricultural production.

[0003] Microbial agents, with their unique biodegradable properties, have gradually become an effective solution to improve straw conversion efficiency. By adding specific microbial flora, the decomposition process of straw can be accelerated and converted into valuable products such as organic fertilizer, feed or biofuel. However, the application effects of many microbial agents on the market are uneven. The strains of some products have low activity and poor adaptability, resulting in conversion efficiency that fails to meet expectations.

[0004] In view of this, we propose a microbial agent for improving straw conversion efficiency and its application. Summary of the invention

[0005] The purpose of the present invention is to provide a microbial agent for improving straw conversion efficiency and its application, aiming to solve the problem that the application effects of many microbial agents on the market are uneven, some products have low strain activity and poor adaptability, resulting in the conversion efficiency failing to reach the expected level.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a microbial agent for improving straw conversion efficiency, wherein the microbial agent incorporates the following microbial populations and additives:

[0007] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.8:1.4;

[0008] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0009] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 3.5% of the total mass of the microbial composition.

[0010] Preferably, the carrier system further comprises a step of pulverizing the rice straw powder before mixing the rice straw powder with the bentonite, so that the particle size of the rice straw powder is within the range of 0.5-1.0 mm.

[0011] Preferably, the carrier system further comprises drying the bentonite and activating it with acid or alkali before mixing the rice straw powder with the bentonite to improve its adsorption capacity and reaction activity.

[0012] Preferably, the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are all subjected to the following screening steps;

[0013] S1: Collect samples containing target microorganisms from the natural environment, place the collected samples in a suitable culture medium for preliminary culture to enrich the target microorganisms;

[0014] S2: Design a selection medium based on the physiological characteristics and metabolic requirements of the target microorganisms, and inoculate the initially cultured microbial samples onto the selection medium for further screening;

[0015] S3: Purify the microorganisms on the selected culture medium by streaking or dilution coating to obtain a single colony. Then, identify the purified microorganisms by microscopic observation, physiological and biochemical tests or molecular biological identification methods to ensure that they are the target microorganisms.

[0016] S4: Evaluate the performance of the purified target microorganisms, including growth rate, degradation ability, and stress resistance. Based on the evaluation results, select microorganisms with excellent performance and improve their degradation efficiency and stability through mutation breeding or genetic engineering.

[0017] S5: Load the target microorganisms that have been screened, purified and evaluated for performance onto the carrier system.

[0018] Preferably, during the screening process of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, each microorganism is subjected to at least three rounds of continuous culture to ensure the stability and consistency of its excellent traits.

[0019] Preferably, during the microbial culture screening process, sampling is performed every 24 hours to evaluate the mutagenic effect.

[0020] Preferably, the microorganism being evaluated for performance is mixed with the carrier system under sterile conditions.

[0021] An application of a microbial agent for improving straw conversion efficiency comprises the microbial agent for improving straw conversion efficiency as described in any one of the above items, characterized in that it is applied in straw conversion.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The microbial agent of the present invention can not only significantly improve the conversion efficiency of straw by optimizing the proportion of microbial population, carrier system and additive ratio, but also improve the soil fertility of crops, realize efficient utilization of resources, and thus promote the sustainable development of agriculture. For example, the straw treated with the microbial agent can achieve efficient biodegradation in a short time, reduce the risk of environmental pollution, and achieve a win-win situation for economy and ecology.

[0024] 2. In the present invention, the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are strictly screened and purified to ensure that they have excellent growth performance and degradation ability on the carrier system. In addition, these microbial populations have good synergistic effects and can jointly promote the efficient degradation of straw. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the composition of the microbial agent of the present invention. DETAILED DESCRIPTION

[0026] Embodiment 1

[0027] like Figure 1 As shown, a microbial agent for improving straw conversion efficiency, the microbial agent combines the following microbial populations and additives:

[0028] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.8:1.4;

[0029] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0030] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 3.5% of the total mass of the microbial composition;

[0031] Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride can effectively degrade cellulose, hemicellulose and lignin components in straw through synergistic action, significantly improving the biodegradability and availability of straw. Saccharomyces cerevisiae is mainly responsible for converting degradation products into valuable biomass energy such as ethanol, while Bacillus amyloliquefaciens and Trichoderma viride can effectively break through the complex structural barriers of straw by secreting extracellular enzymes, accelerate the decomposition process, and achieve efficient recycling of resources.

[0032] The mixing ratio of rice straw powder and bentonite in the carrier system has been carefully optimized. Rice straw powder provides a substrate environment that is familiar and easy to use for microorganisms, promoting their rapid adaptation and reproduction; bentonite, due to its good water absorption, water retention and ion exchange capacity, helps to maintain the stability of the microenvironment around the microbial cells, further improving the survival rate and activity of the microorganisms.

[0033] The mixture of additives xanthan gum and sodium carboxymethyl cellulose not only enhances the physical stability of the microbial agent and prevents the loss of the bacteria during storage and transportation, but also forms a protective film on the surface of the straw through its unique viscosity and film-forming properties, which not only protects the microorganisms from the influence of the adverse external environment, but also promotes close contact between the microorganisms and the straw, accelerating the degradation reaction.

[0034] Embodiment 2

[0035] This embodiment is basically the same as the first embodiment, except for the ratio of live bacteria of Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride. Different microorganisms play different roles in the fermentation process. Saccharomyces cerevisiae is mainly responsible for the conversion of sugars, while Bacillus amyloliquefaciens and Trichoderma viride can help degrade more complex carbohydrates and optimize the overall efficiency of fermentation.

[0036] A microbial agent for improving straw conversion efficiency, the microbial agent combines the following microbial populations and additives:

[0037] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.5:0.9;

[0038] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0039] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 3.5% of the total mass of the microbial composition.

[0040] Embodiment 3

[0041] This embodiment is basically the same as the first embodiment, except for the ratio of live bacteria of Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride. Different microorganisms play different roles in the fermentation process. Saccharomyces cerevisiae is mainly responsible for the conversion of sugars, while Bacillus amyloliquefaciens and Trichoderma viride can help degrade more complex carbohydrates and optimize the overall efficiency of fermentation.

[0042] A microbial agent for improving straw conversion efficiency, the microbial agent combines the following microbial populations and additives:

[0043] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 0.8:1:1.1;

[0044] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0045] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 3.5% of the total mass of the microbial composition.

[0046] Embodiment 4

[0047] This comparative example is basically the same as Example 1, except that the mass ratio of rice straw powder to bentonite is different in this example;

[0048] A microbial agent for improving straw conversion efficiency, the microbial agent combines the following microbial populations and additives:

[0049] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.8:1.4;

[0050] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1.5, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0051] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 3.5% of the total mass of the microbial composition.

[0052] Embodiment 5

[0053] This comparative example is basically the same as Example 1, except that the mass ratio of xanthan gum to sodium carboxymethyl cellulose is different in this example;

[0054] A microbial agent for improving straw conversion efficiency, the microbial agent combines the following microbial populations and additives:

[0055] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.8:1.4;

[0056] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0057] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1:1.3, and the total amount of the additive added is 3.5% of the total mass of the microbial composition.

[0058] Embodiment 6

[0059] This comparative example is basically the same as Example 1, except that the total amount of additives added in this example is different;

[0060] A microbial agent for improving straw conversion efficiency, the microbial agent combines the following microbial populations and additives:

[0061] The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.8:1.4;

[0062] The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell;

[0063] The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 5% of the total mass of the microbial composition.

[0064] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. A microbial agent for improving straw conversion efficiency, characterized in that: The microbial agent combines the following microbial populations and additives: The microbial population includes screened Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride, wherein the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are loaded on the same carrier system, and the ratio of live bacteria of the Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride is 1:1.8:1.4; The carrier system is made of rice straw powder and bentonite in an optimized mass ratio of 1:1, aiming to provide a habitat and reproduction environment for microorganisms, ensuring that at least 1×10 7 A highly active and stable microbial cell; The additive is a mixture of xanthan gum and sodium carboxymethyl cellulose, wherein the mass ratio of xanthan gum to sodium carboxymethyl cellulose is 1.2:1, and the total amount of the additive added is 3.5% of the total mass of the microbial composition.

2. A microbial agent for improving straw conversion efficiency according to claim 1, characterized in that: The carrier system further comprises a step of pulverizing the rice straw powder before mixing the rice straw powder with the bentonite, so that the particle size of the rice straw powder is within the range of 0.5-1.0 mm.

3. A microbial agent for improving straw conversion efficiency according to claim 1, characterized in that: The carrier system also includes drying the bentonite before mixing the rice straw powder with the bentonite, and activating the bentonite with acid or alkali to improve its adsorption capacity and reaction activity.

4. A microbial agent for improving straw conversion efficiency according to claim 1, characterized in that: The Saccharomyces cerevisiae, Bacillus amyloliquefaciens and Trichoderma viride are all subjected to the following screening steps; S1: Collect samples containing target microorganisms from the natural environment, place the collected samples in a suitable culture medium for preliminary culture to enrich the target microorganisms; S2: Design a selection medium based on the physiological characteristics and metabolic requirements of the target microorganisms, and inoculate the initially cultured microbial samples onto the selection medium for further screening; S3: Purify the microorganisms on the selected culture medium by streaking or dilution coating to obtain a single colony. Then, identify the purified microorganisms by microscopic observation, physiological and biochemical tests or molecular biological identification methods to ensure that they are the target microorganisms. S4: Evaluate the performance of the purified target microorganisms, including growth rate, degradation ability, and stress resistance. Based on the evaluation results, select microorganisms with excellent performance and improve their degradation efficiency and stability through mutation breeding or genetic engineering. S5: Load the target microorganisms that have been screened, purified and evaluated for performance onto the carrier system.

5. A microbial agent for improving straw conversion efficiency according to claim 4, characterized in that: During the screening process of the brewer's yeast, Bacillus amyloliquefaciens and Trichoderma viride, each microorganism was screened through at least three rounds of continuous culture to ensure the stability and consistency of its excellent traits.

6. A microbial agent for improving straw conversion efficiency according to claim 4, characterized in that: During the microbial culture screening process, sampling was performed every 24 hours to evaluate the mutagenic effect.

7. The microbial agent for improving straw conversion efficiency according to claim 4, characterized in that: The microorganisms undergoing performance evaluation are mixed with the carrier system under sterile conditions.

8. An application of a microbial agent for improving straw conversion efficiency, comprising the microbial agent for improving straw conversion efficiency as claimed in any one of claims 1 to 7, characterized in that: For application in straw conversion.

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