Apple peptide composition with weight-losing and lipid-lowering effects and preparation method thereof

Abstract

The apple peptide composition provided by the invention is prepared from the following components in parts by mass: 3 to 5 parts of # imgabs0 # apple dipeptide, 0.5 to 1 part of compound prebiotics, 20 to 25 parts of apple juice, 0.5 to 1 part of fructus hippophae extract, 0.1 to 0.3 part of sodium carboxymethyl cellulose, 0.05 to 0.1 part of sodium acetate, 0.1 to 0.5 part of potassium sorbate and 50 to 60 parts of deionized water, the compound prebiotics are prepared from polydextrose, lactitol, fructo-oligosaccharide, resistant dextrin, isomaltooligosaccharide, stachyose, xylitol, soybean oligosaccharide and galactooligosaccharide according to the mass ratio of (1 to 3): (5 to 6): (15 to 20): (2 to 4): (1 to 3): (1 to 3): (6 to 8): (2 to 5): (2 to 4). The apple peptide composition disclosed by the invention is simple in preparation process, can achieve good weight-losing and lipid-lowering effects, can relieve the problems of intestinal discomfort, allergy and the like caused by the addition of prebiotics, avoids the generation of precipitates, is suitable for various crowds, and is wide in application range.

Description

Technical Field

[0001] The invention belongs to the technical field of health care products, and particularly relates to an apple peptide composition with weight loss and lipid-lowering effects and a preparation method thereof. Background Art

[0002] In modern society, due to bad eating habits and lifestyles, more and more people are suffering from obesity. Obesity not only affects personal image, but also leads to a variety of health problems, such as heart disease, high blood pressure and diabetes. The main cause of obesity is that the calorie intake is greater than the calorie consumption, which causes the continuous accumulation of fat. According to research and investigation, the increase in fat content is proportional to the degree of obesity. Too much fat will bring a lot of trouble to obese people, such as inconvenience in movement, fear of heat, affecting body shape, easy to get tired, and susceptible to various diseases. Therefore, weight loss and lipid reduction can keep the body fat rate within the normal range, which plays an important role in people's health.

[0003] The methods of weight loss and lipid reduction mainly include controlling diet, taking medicine, strengthening exercise, liposuction and the like. Among them, controlling diet and strengthening exercise require great perseverance to complete, and the effect is slow, while liposuction is expensive and easy to cause damage to the body. Therefore, few people will choose the above methods to achieve weight loss and lipid reduction. At present, the most commonly used method is to take related drugs. Existing drugs with weight loss and lipid reduction effects include liraglutide, orlistat, metformin, octreotide, fintrexone and the like. Taking too much of these drugs will cause symptoms such as diarrhea, vitamin deficiency, headache, nausea, and insomnia. Affecting human health. Therefore, it is very important for obese people to develop a composition that is harmless to the human body and has the effect of weight loss and lipid reduction.

[0004] Chinese patent application CN118058457A discloses a nutritious meal replacement powder for weight loss and lipid reduction, comprising dietary fiber, nutrients and compound nutrient enhancers, wherein the nutrients include compound prebiotics, macroelements and a variety of trace elements, the prebiotics include one of crystalline fructose, arabinose, chitosan, oligofructose, galacto-oligosaccharide or xylo-oligosaccharide, isomaltooligosaccharide, spirulina, arthrospira, coriolus versicolor polysaccharide, carrot nitrogen-containing polysaccharide, etc. This nutritious powder has the effect of weight loss and lipid reduction, but this nutritious meal replacement powder is added with a large amount of prebiotics, and long-term and large-scale use can easily cause allergies, diarrhea and other problems, affecting human health.

[0005] Duoyanshou teamed up with the American HMG laboratory to successfully create Apple dipeptide technology. Apple dipeptide is a short-chain bioactive peptide extracted from red apples and green apples by HMG laboratory from more than 70 apple varieties around the world. It uses biomedical and biosynthetic cross technology to finally obtain a product with the dual effects of "burning fat and controlling sugar". Black technology for weight loss. But the existing Apple dipeptide products generally have the problem of poor stability, which affects the weight loss effect. The application of apple dipeptide products is limited.

[0006] In summary, although the drugs in the prior art can achieve the effect of weight loss and lipid reduction, they generally have poor stability and are prone to cause intestinal discomfort, leading to diarrhea, allergies, etc., which affect human health. Therefore, how to solve the above problems is of great significance for further expanding the application of weight loss and lipid reduction products. Summary of the invention

[0007] In view of the common problems of the prior art, the present invention provides an apple peptide composition with weight loss and lipid-lowering effects and a preparation method thereof. The apple peptide composition provided by the present invention has good weight loss and lipid-lowering effects and good stability, and can protect the stomach and intestines, avoid allergies, and help maintain people's health.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] An apple peptide composition with weight loss and lipid-lowering effects comprises the following components and their weight fractions: 3-5 parts of apple dipeptide, 0.5-1 part of compound prebiotics, 20-25 parts of apple juice, 0.5-1 part of seabuckthorn extract, 0.1-0.3 part of sodium carboxymethyl cellulose, 0.05-0.1 part of sodium acetate, 0.1-0.5 part of potassium sorbate, and 50-60 parts of deionized water.

[0010] The apple dipeptide of the present invention adopts Apple dipeptide. In the specific research and development process, Apple double peptides combined with compound prebiotics can achieve good weight loss and lipid-lowering effects, but in further experiments, the inventors found that when the two are used at the same time, The protein in apple dipeptide and the polysaccharide in compound prebiotics interact easily to form complex coagulation, forming insoluble complexes (i.e., precipitation), which affects the stability of the product. Based on this, the inventor conducted in-depth research and found that adding a small amount of sodium carboxymethyl cellulose and sodium acetate can effectively block the The complex coagulation of the apple dipeptide and the protein, polysaccharide and other substances in the composite prebiotics is helpful to improve the stability of the composition. The apple peptide composition prepared by the present invention can be placed at 40°C for more than 6 months and still has a good effect after 6 months, which proves that the product prepared by the present invention has high stability.

[0011] Preferably, the apple peptide composition comprises the following components and their mass fractions: 4 parts of apple dipeptide, 0.7 parts of compound prebiotics, 23 parts of apple juice, 0.8 parts of seabuckthorn extract, 0.2 parts of sodium carboxymethyl cellulose, 0.07 parts of sodium acetate, 0.3 parts of potassium sorbate, and 55 parts of deionized water.

[0012] Preferably, the composite prebiotics are composed of polydextrose, lactitol, fructo-oligosaccharides, resistant dextrin, isomaltooligosaccharides, stachyose, xylitol, soybean oligosaccharides and galacto-oligosaccharides in the mass ratio of 1-3:5-6:15-20:2-4:1-3:1-3:6-8:2-5:2-4.

[0013] Preferably, the composite prebiotic is composed of polydextrose, lactitol, fructooligosaccharides, resistant dextrin, isomaltooligosaccharides, stachyose, xylitol, soybean oligosaccharides and galacto-oligosaccharides in a weight ratio of 2:5:17:3:2:2:7:4:3.

[0014] Preferably, the extraction method of the seabuckthorn extract is as follows: wash and freeze the seabuckthorn fruits, then crush the seabuckthorn fruits, put them into an extractor, add 70% ethanol until the fruits are covered, then stir at 40-45°C and 50-100rpm for 2-3h, then take the supernatant, concentrate it, and dry it.

[0015] Preferably, the apple peptide composition further comprises 0.05 to 0.1 parts of glutathione.

[0016] Prebiotics can supplement the human body with substances such as sugars and various dietary fibers, which are conducive to promoting the digestion and absorption of the intestines and avoiding the accumulation of fat. However, long-term use of prebiotics is likely to cause irritation to the stomach and overdraw the stomach and intestines. In order to solve this problem, the inventor further studied the ingredients of the composition and unexpectedly found that adding a trace amount of glutathione, which cooperates with the seabuckthorn extract, can effectively solve the above problems. This is because the flavonoid compounds and beneficial fatty acids in the seabuckthorn extract interact with glutathione to form a protective film, which protects the stomach and intestines from irritation during drinking and avoids allergies. In addition, the seabuckthorn extract of the present invention also contains nutrients such as multivitamins, proteins, cellulose and ash elements that are of great benefit to the human body, which are sufficient for the human body to supplement, help improve the body's resistance, and further ensure human health.

[0017] The present invention also provides a method for preparing the apple peptide composition, comprising the following steps:

[0018] S1, dissolving apple dipeptide and glutathione in deionized water and mixing to obtain a mixture I;

[0019] S2, mixing apple juice, sea buckthorn extract and potassium sorbate and stirring them evenly, and then adding them to the mixture I prepared in step S1 to obtain a mixture II;

[0020] S3. Add the composite prebiotics to the mixture II obtained in step S2, and add the remaining amount of deionized water, mix and stir evenly, then add sodium acetate and sodium carboxymethyl cellulose, and adjust the pH to 4-4.5 to obtain the mixture.

[0021] Preferably, the mixing and stirring conditions in step S2 are stirring at a speed of 300 to 500 rpm for 20 to 30 minutes.

[0022] Preferably, the mixing and stirring conditions in step S3 are stirring at a speed of 800 to 1000 rpm for 30 to 40 minutes.

[0023] Compared with the prior art, the present invention has the following advantages:

[0024] (1) The apple dipeptide composition provided by the present invention can effectively promote fat decomposition, increase metabolic rate, and help lose weight and reduce fat;

[0025] (2) The apple dipeptide composition provided by the present invention can supplement various nutrients such as dietary fiber, vitamins, proteins, etc. for the human body, effectively improve the digestion and absorption function of the intestine, help to improve the body's resistance, and be beneficial to health;

[0026] (3) The apple dipeptide composition provided by the present invention has a simple preparation method, low production cost, good stability, and will not cause irritation to the stomach during drinking, and is not allergic, and can be widely promoted and applied. DETAILED DESCRIPTION

[0027] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] The glutathione can be purchased from Guangdong Mingcheng Biotechnology Co., Ltd. with a CAS number of 70-18-8; the apple juice is concentrated juice and can be purchased from Hebei Greenno Food Co., Ltd. with a production license number of SC10613013300301; the resistant dextrin can be purchased from Qingdao Haiweisen Biotechnology Co., Ltd. with a product number of 164418044.

[0029] Example 1 An apple peptide composition with weight loss and lipid-lowering effects

[0030] The apple peptide composition with weight loss and lipid-lowering effects is composed of the following components and their mass fractions: 3 parts of apple dipeptide, 0.5 parts of compound prebiotics, 20 parts of apple juice, 0.5 parts of sea buckthorn extract, 0.1 parts of sodium carboxymethyl cellulose, 0.05 parts of sodium acetate, 0.1 parts of potassium sorbate, 0.05 parts of glutathione, and 50 parts of deionized water; the compound prebiotics are composed of polydextrose, lactitol, fructooligosaccharides, resistant dextrin, isomaltose, stachyose, xylitol, soybean oligosaccharides and galacto-oligosaccharides in the ratio of 1:5:15:2:1:1:6:2:2 by mass.

[0031] The seabuckthorn extract of the present invention is extracted according to the following method: wash and freeze the seabuckthorn fruits, then crush the seabuckthorn fruits, put them into an extractor, add 70% ethanol until the fruits are covered, then stir at 40°C and 100rpm for 3h, then take the supernatant, concentrate it, and dry it.

[0032] The preparation method of the apple peptide composition comprises the following steps:

[0033] S1, dissolving apple dipeptide and glutathione in deionized water and mixing to obtain a mixture I;

[0034] S2, mixing apple juice, sea buckthorn extract and potassium sorbate, stirring at 300 rpm for 30 min until uniform, and then adding the mixture to the mixture I prepared in step S1 to obtain a mixture II;

[0035] S3. Add the composite prebiotics to the mixture II obtained in step S2, and add the remaining amount of deionized water, mix, stir at 800 rpm for 40 min until uniform, then add sodium acetate and sodium carboxymethyl cellulose, and adjust the pH to 4 to obtain the mixture.

[0036] Example 2 An apple peptide composition with weight loss and lipid-lowering effects

[0037] The apple peptide composition with weight loss and lipid-lowering effects includes the following components and their mass fractions: 5 parts of apple dipeptide, 1 part of compound prebiotics, 25 parts of apple juice, 1 part of sea buckthorn extract, 0.3 parts of sodium carboxymethyl cellulose, 0.1 parts of sodium acetate, 0.5 parts of potassium sorbate, 0.1 parts of glutathione, and 60 parts of deionized water; the compound prebiotics are composed of polydextrose, lactitol, fructooligosaccharides, resistant dextrin, isomaltose, stachyose, xylitol, soybean oligosaccharides and galacto-oligosaccharides in the ratio of 3:5:20:4:3:3:8:5:4 by mass.

[0038] The extraction method of the seabuckthorn extract is as follows: wash and freeze the seabuckthorn fruits, then crush the seabuckthorn fruits, put them into an extractor, add 70% ethanol until the fruits are covered, then stir at 45°C and 100rpm for 2h, then take the supernatant, concentrate it, and dry it.

[0039] The preparation method of the apple peptide composition comprises the following steps:

[0040] S1, dissolving apple dipeptide and glutathione in deionized water and mixing to obtain a mixture I;

[0041] S2, mixing apple juice, sea buckthorn extract and potassium sorbate, stirring at 500 rpm for 20 min until uniform, and then adding the mixture to the mixture I prepared in step S1 to obtain a mixture II;

[0042] S3. Add the composite prebiotics to the mixture II obtained in step S2, and add the remaining amount of deionized water, mix and stir at a speed of 1000 rpm for 30 minutes until uniform, then add sodium acetate and sodium carboxymethyl cellulose, and adjust the pH to 4.5 to obtain the mixture.

[0043] Example 3 An apple peptide composition with weight loss and lipid-lowering effects

[0044] The apple peptide composition with weight loss and lipid-lowering effects is composed of the following components and their mass fractions: 4 parts of apple dipeptide, 0.7 parts of compound prebiotics, 23 parts of apple juice, 0.8 parts of sea buckthorn extract, 0.2 parts of sodium carboxymethyl cellulose, 0.07 parts of sodium acetate, 0.3 parts of potassium sorbate, 0.08 parts of glutathione, and 55 parts of deionized water; the compound prebiotics are composed of polydextrose, lactitol, fructooligosaccharides, resistant dextrin, isomaltose, stachyose, xylitol, soybean oligosaccharides and galacto-oligosaccharides in the ratio of 2:5:18:3:22:7:4:3 by mass.

[0045] The extraction method of the seabuckthorn extract is as follows: wash and freeze the seabuckthorn fruits, then crush the seabuckthorn fruits, put them into an extractor, add 70% ethanol until the fruits are covered, then stir at 43°C and 80rpm for 2.5h, then take the supernatant, concentrate it, and dry it.

[0046] The preparation method of the apple peptide composition comprises the following steps:

[0047] S1, dissolving apple dipeptide and glutathione in deionized water and mixing to obtain a mixture I;

[0048] S2, mixing apple juice, sea buckthorn extract and potassium sorbate, stirring at 400 rpm for 25 min until the mixture is uniformly mixed, and then adding the mixture to the mixture I prepared in step S1 to obtain a mixture II;

[0049] S3. Add the composite prebiotics to the mixture II obtained in step S2, add the remaining amount of deionized water and mix, stir at 900 rpm for 35 min until the mixture is uniformly mixed, then add sodium acetate and sodium carboxymethyl cellulose, and adjust the pH to 4.2 to obtain the mixture.

[0050] Comparative Example 1: An apple peptide composition

[0051] The composition and preparation method of the apple peptide composition are similar to those in Example 3;

[0052] The difference from Example 3 is that in Comparative Example 1, sodium carboxymethyl cellulose is replaced by sodium acetate.

[0053] Comparative Example 2: An apple dipeptide composition

[0054] The composition and preparation method of the apple dipeptide composition are similar to those in Example 3;

[0055] The difference from Example 3 is that in Comparative Example 2, sodium acetate is replaced by sodium carboxymethyl cellulose.

[0056] Comparative Example 3: An apple dipeptide composition

[0057] The composition and preparation method of the apple dipeptide composition are similar to those in Example 3;

[0058] The difference from Example 3 is that in Comparative Example 3, glutathione is replaced by vitamin C.

[0059] Comparative Example 4: An apple dipeptide composition

[0060] The composition and preparation method of the apple dipeptide composition are similar to those in Example 3;

[0061] The difference from Example 3 is that the seabuckthorn extract in Comparative Example 4 is replaced by seabuckthorn leaf extract, which can be purchased from Xi'an Lanzhixing Biotechnology Co., Ltd. with a single product number of XALZX-252.

[0062] Test Example 1 Stability Test

[0063] 1. Test sample: apple dipeptide composition prepared in Examples 1-3 and Comparative Examples 1-4;

[0064] 2. Test process: Take 100 mL of each of the above samples and divide each sample into 2 portions (50 mL each), one for high temperature test and the other for low temperature test: The high temperature test is conducted by placing each sample in a constant temperature box at 40±1℃, and the low temperature test is conducted by placing each sample in a constant temperature box at -5℃±1℃. Both samples are placed away from light for 6 months, and the changes in appearance are observed after 6 months, including whether there is stratification, color change, and whether flocs appear.

[0065] 3. Test results: The specific test results are shown in Table 1 below:

[0066] Table 1 Stability test results of different test samples

[0067] Group Whether to layer Color Change Is there flocculent Example 1 Group No stratification Unchanged No flocs Example 2 Group No stratification Unchanged No flocs Example 3 Group No stratification Unchanged No flocs Comparative Example 1 There is stratification Slightly changed A small amount of flocculent Comparative Example 2 There is stratification Slightly changed A small amount of flocculent Comparative Example 3 No stratification Unchanged No flocs Comparative Example 4 No stratification Unchanged No flocs

[0068] It can be seen from Table 1 that the apple dipeptide compositions prepared in Examples 1-3 of the present invention still have excellent stability after being placed at 40° C. for 6 months, while the comparative examples 1-4 all have instability.

[0069] Test Example 2 Gastrointestinal irritation test

[0070] 1. Test sample: apple dipeptide composition prepared in Examples 1-3 and Comparative Examples 1-4;

[0071] 2. Experimental animals: 35 healthy SD rats, weighing 250±5g, 5 rats in each group;

[0072] 3. Test method: Each group used the apple dipeptide composition of Examples 1 to 3 and Comparative Examples 1 to 4 to gavage SD rats, with a gavage volume of 3 mL and a gavage flow rate of 0.08 mL / min for 10 consecutive days. During the gavage period, the rats were observed to see if they had gastric retention. After 10 days of gavage, the SD rats were euthanized and the gastric mucosa and intestinal mucosa of the SD rats were observed by sectioning.

[0073] 4. Test results: The specific test results are shown in Table 2 below:

[0074] Table 2 Gastrointestinal irritation results of different test samples

[0075]

[0076] As can be seen from Table 2, the SD rats that took the apple dipeptide composition of Examples 1-3 of the present invention had normal gastrointestinal function and no irritation after taking it for 10 consecutive days. However, the control group destroyed the integrity of the composition, resulting in different degrees of gastrointestinal irritation. In particular, the control group 3-4 did not contain glutathione, which destroyed the interaction between glutathione and the seabuckthorn extract, resulting in more obvious gastrointestinal irritation during the taking process compared with the other groups, which further proves the synergistic promotion effect between the components of the present invention.

[0077] Test Example 3 Weight Loss and Lipid Lowering Efficacy Test

[0078] 1. Test sample: apple dipeptide composition prepared in Examples 1-3 and Comparative Examples 1-2;

[0079] 2. Experimental animals: A total of 70 SD male rats weighing about 150±10g were divided into 7 groups, with 10 rats in each group. All rats were in good health and had no obvious problems.

[0080] 3. Experimental method: The above-mentioned SD rats were divided into a blank control group, an obesity model group, Example 1-3 groups, and Comparative Example 1-2 groups. The treatment methods of each group were as follows: the blank control group was fed with ordinary feed, and the mice in the other groups were given high-fat feed (purchased from Synergy Bio, 60% fat energy high-fat feed (reference D12492), product number XTHF60). During the feeding process, sufficient feed was ensured. The SD rats were free to eat and were fed for 7 consecutive weeks to establish a nutritional obesity model. When the weight gain of the mice exceeded that of the blank control group by more than 20%, the modeling was considered successful.

[0081] After the modeling was successful, starting from the 8th week, each group was given an equal amount of apple dipeptide composition of Examples 1-3 and Comparative Examples 1-2, 10 mL / kg.BW per day, and the blank control group and the obesity model group were gavaged with a corresponding volume of normal saline. During this period, each group was fed regularly and quantitatively (fed once every 4 hours, 0.8 g per animal each time), the blank control group continued to be fed with ordinary feed, and the others continued to be fed with high-fat feed.

[0082] Two weeks after the experiment, 12 hours after the last gavage, the mice were weighed and then fasted for 12 hours. Blood was collected from the eye sockets, and serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were quantitatively detected using an enzymatic method on an automatic biochemical analyzer.

[0083] 4. Experimental results: The changes in body weight of each group of mice (the average value of 10 SD rats in each group) and the levels of triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol (the average value of 10 SD rats in each group) are shown in Tables 3 and 4.

[0084] Table 3 Body weight changes of SD rats in different experimental groups

[0085] Group Initial average weight Average weight after 7 weeks Average weight after 9 weeks Example 1 Group 151.37g 330.95g 362.18g Example 2 Group 155.65g 326.88g 357.55g Example 3 Group 159.78g 346.41g 369.46g Comparative Example 1 153.05g 325.67g 374.22g Comparative Example 2 157.55g 363.94g 401.68g Blank control group 145.94g 285.15g 338.42g Model control group 151.13g 329.42g 395.48g

[0086] Table 4 Results of blood biochemical parameters of rats in each group

[0087]

[0088]

[0089] As shown in Tables 3 to 4 above, after 7 weeks of free feeding, the weight gain of the Example 1-3 group, the Comparative Example 1-2 group and the model control group of the present invention was 20% higher than that of the blank control group, indicating that the modeling was successful. As shown in Table 3, compared with the model control group, the weight gain of the Example 1-3 group after 9 weeks was significantly lower than that of the Comparative Example 1-2 group compared with the first 7 weeks, indicating that the apple dipeptide composition prepared by the present invention has a good weight loss effect. As shown in Table 4, after testing, the content of triglycerides, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol in the Example 1-3 group of the present invention was lower than that in the Comparative Example 1-2 group, proving that the apple dipeptide composition prepared by the present invention has a lipid-lowering effect. It can be seen that the apple dipeptide composition prepared by the present invention has a fat-reducing effect.

[0090] Finally, it should be noted that the above embodiments are only illustrative of the principles, performance and efficacy of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or change the above embodiments without violating the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical ideas disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. An apple peptide composition having weight loss and lipid-lowering effects, characterized in that: The invention comprises the following components and their weight proportions: 3-5 parts of apple dipeptide, 0.5-1 part of compound prebiotics, 20-25 parts of apple juice, 0.5-1 part of seabuckthorn extract, 0.1-0.3 part of sodium carboxymethyl cellulose, 0.05-0.1 part of sodium acetate, 0.1-0.5 part of potassium sorbate and 50-60 parts of deionized water.

2. The apple peptide composition according to claim 1, characterized in that The invention comprises the following components and their weight proportions: 4 parts of apple dipeptide, 0.7 parts of compound prebiotics, 23 parts of apple juice, 0.8 parts of seabuckthorn extract, 0.2 parts of sodium carboxymethyl cellulose, 0.07 parts of sodium acetate, 0.3 parts of potassium sorbate and 55 parts of deionized water.

3. The apple peptide composition according to claim 1, characterized in that The composite prebiotics are composed of polydextrose, lactitol, oligofructose, resistant dextrin, oligomaltose, stachyose, xylitol, soybean oligosaccharide and oligogalactose in the mass ratio of 1-3:5-6:15-20:2-4:1-3:1-3:6-8:2-5:2-4.

4. The apple peptide composition according to claim 3, characterized in that: The composite prebiotics are composed of polydextrose, lactitol, fructo-oligosaccharides, resistant dextrin, isomaltooligosaccharides, stachyose, xylitol, soybean oligosaccharides and galacto-oligosaccharides in the weight ratio of 2:5:17:3:2:2:7:4:

3.

5. The apple peptide composition according to claim 1, characterized in that The extraction method of the seabuckthorn extract is as follows: wash and freeze the seabuckthorn fruits, then crush the seabuckthorn fruits, put them into an extractor, add 70% ethanol until the fruits are covered, then stir at 40-45°C and 50-100rpm for 2-3h, then take the supernatant, concentrate it, and dry it.

6. The apple peptide composition according to any one of claims 1 to 5, characterized in that: The apple peptide composition also includes 0.05 to 0.1 parts of glutathione.

7. A method for preparing the apple peptide composition as claimed in claim 6, characterized in that: The steps include: S1, dissolving apple dipeptide and glutathione in deionized water and mixing to obtain a mixture I; S2, mixing apple juice, sea buckthorn extract and potassium sorbate and stirring them evenly, and then adding them to the mixture I prepared in step S1 to obtain a mixture II; S3. Add the composite prebiotics to the mixture II obtained in step S2, and add the remaining amount of deionized water, mix and stir evenly, then add sodium acetate and sodium carboxymethyl cellulose, and adjust the pH to 4-4.5 to obtain the mixture.

8. The preparation method according to claim 7, characterized in that: The mixing and stirring conditions described in step S2 are stirring at a speed of 300 to 500 rpm for 20 to 30 minutes.

9. The preparation method according to claim 7, characterized in that: The mixing and stirring conditions in step S3 are stirring at a speed of 800 to 1000 rpm for 30 to 40 minutes.

Images