Ginger root stock solution for promoting scalp blood microcirculation and preparation method and application thereof

Abstract

The invention belongs to the technical field of plant processing and extraction, and particularly relates to a ginger root stock solution for promoting scalp blood microcirculation and a preparation method and application thereof. The invention further provides a preparation method of the ginger root stock solution for promoting scalp blood microcirculation. The preparation method comprises the following steps: S1, pretreating ginger roots to obtain ginger roots to be squeezed; s2, squeezing the ginger roots to be squeezed by using a squeezer, and collecting to obtain ginger root liquid A; s3, adding a preservative into the ginger root solution A, and then adjusting the pH value to obtain a ginger root solution B; s4, centrifuging the ginger root liquid B to obtain supernatant; and S5, adding a suspending agent into the supernatant to obtain the ginger root stock solution. The invention also provides the ginger root stock solution obtained by the preparation method. A squeezing method is applied to preparation of the ginger root extract, 100% ginger root stock solution is obtained, and more active ingredients are reserved compared with a water extraction method; the ginger root stock solution disclosed by the invention has the effects of inhibiting 5alpha-reductase and promoting blood microcirculation.

Description

Technical Field

[0001] The invention belongs to the technical field of plant processing and extraction, and particularly relates to a ginger root stock solution for promoting scalp blood microcirculation, and a preparation method and application thereof. Background Art

[0002] Among the ginger plants, ginger is a traditional Chinese medicine and is also commonly used in food. Its efficacy and safety are widely recognized by the public. The chemical components of ginger mainly include three categories: volatile oil, gingerol, and diphenylheptane. Volatile oil is mainly terpenoids, and mainly sesquiterpenes; gingerol includes gingerol, shogaol, gingerone, gingerdione, etc. In the cosmetics industry, ginger extract can be used to prepare essential oils, water extracts, etc. The commonly used preparation processes are steam distillation, solvent extraction and other preparation methods. Such preparation processes cannot obtain 100% ginger root stock solution.

[0003] Ginger extract is commonly used to prevent hair loss. However, the mechanism of hair loss is extremely complex, including the formation of DHT with stronger binding activity to hair papilla cells after 5α-reductase reduces androgen, causing hair follicle atrophy; and the obstruction of blood microcirculation in the scalp hair follicles, which leads to poor nutrient supply to the hair follicles and hair loss. Therefore, how to prepare a ginger extract with a high content of active ingredients that can inhibit 5α-reductase and promote blood microcirculation has been a technical problem that needs to be solved in this field in recent years. Summary of the invention

[0004] In order to solve the above technical problems, the present invention adopts the following technical solutions: The present invention provides a method for preparing a ginger root stock solution for promoting scalp blood microcirculation, comprising the following steps: S1. Pre-treating ginger roots to obtain ginger roots to be squeezed; S2, squeezing the ginger root to be squeezed with a squeezer to collect and obtain ginger root liquid A; S3, adding a preservative to the ginger root liquid A, adjusting the pH to obtain ginger root liquid B; S4, centrifuging the ginger root liquid B to obtain a supernatant; S5. Add a suspending agent to the upper layer liquid to obtain ginger root stock solution.

[0005] Furthermore, the ginger root includes the root of a plant of the Zingiberaceae family.

[0006] Furthermore, the ginger root includes one or more of Alpinia galanga and Gingerbread.

[0007] Furthermore, the suspending agent includes one or both of xanthan gum and hydroxypropyl methylcellulose.

[0008] Furthermore, the mass ratio of the suspending agent to the ginger root liquid A is 0.1% to 0.3%.

[0009] Furthermore, the press in step S2 uses a pressing plate as a pressing component; the pressing pressure in step S2 includes 10-15 MPa; the single pressing time in step S2 includes 20 minutes; the number of pressings in step S2 includes 5 times; the centrifugal speed in step S4 includes 3000 rpm, and the time includes 30 minutes.

[0010] Furthermore, the pretreatment in step S1 includes washing, drying, disinfecting, and cutting in sequence; the preservative in step S3 includes a mixture of phenoxyethanol and sodium benzoate, or a mixture of phenoxyethanol, benzoic acid, and dehydroacetic acid; step S3 adjusts the pH to 5.5; step S5 includes: adding the suspending agent to the upper layer liquid, mixing evenly, and obtaining the ginger root stock solution.

[0011] Furthermore, the slicing in step S1 includes dicing; the mass ratio of the preservative in step S3 to the ginger root liquid A in step S2 includes 1% to 1.1%; compared with the mass of the ginger root liquid A, the preservative includes a mixture of 0.8% by mass of phenoxyethanol and 0.2% by mass of sodium benzoate, or a mixture of 0.9% by mass of phenoxyethanol, 0.12% by mass of benzoic acid and 0.08% by mass of dehydroacetic acid; citric acid is used to adjust the pH in step S3; and the mixing method in step S5 includes stirring.

[0012] Correspondingly, the present invention also provides a ginger root stock solution obtained by the preparation method of the ginger root stock solution for promoting scalp blood microcirculation as described above, wherein the solid content of the ginger root stock solution comprises 2.60% to 3.76%.

[0013] Accordingly, the present invention also provides a use of the ginger root stock solution as described above, for preparing one or more of a preparation for inhibiting 5α-reductase and a preparation for promoting scalp blood microcirculation.

[0014] Furthermore, the invention can also be used for preparing scalp care products with the effect of preventing hair loss.

[0015] Furthermore, when the ginger root is Alpinia officinalis, the mass ratio of the suspending agent to the ginger root liquid A in step S2 is 0.1% compared to the mass of the ginger root liquid A.

[0016] Furthermore, compared with the mass of the ginger root liquid A, when the ginger root is Yunnan small yellow ginger, the mass ratio of the suspending agent to the ginger root liquid A in step S2 is 0.3%.

[0017] Furthermore, the ginger root used in the present invention is fresh ginger root.

[0018] Furthermore, the temperature of the preparation method of the present invention is room temperature.

[0019] Furthermore, the solid content is calculated by mass.

[0020] Furthermore, the solid content determination method of the ginger root stock solution in the present invention is as follows: take out a weighing bottle from the dryer, weigh it and record it as m1, add an appropriate amount of sample (ginger root stock solution), weigh it and record it as m2, put it into an oven, bake it at 105°C for 3h, take it out, put it into the dryer to cool, weigh it and record it as m3, solid content = (m3-m1) / (m2-m1)*100%, and calculate the solid content according to the solid content formula.

[0021] The preparation method of the present invention adopts a pressing method without solvent extraction to obtain 100% ginger root stock solution (the ginger root includes one or more of Alpinia galanga and Yunnan small yellow ginger).

[0022] The mixed solution obtained after squeezing in the present invention has residues and the like. Although centrifugation can remove starch, protein and residues, the supernatant after centrifugation still has precipitation after long-term storage. The addition of suspending agents such as xanthan gum in the present invention can make the system more uniform and stable, and there is no precipitation after storage. The use of suspending agents stabilizes more water-insoluble active substances, such as phellandrene and α-terpinene. Therefore, the ginger root stock solution obtained by the method of the present invention has a higher content of active substances than the solution obtained by water extraction and conventional squeezing methods, and its effect of inhibiting 5α-reductase and promoting blood microcirculation is also better.

[0023] Compared with the prior art, the implementation of the present invention has the following beneficial effects: 1. The pressing method for extracting active ingredients from ginger or Alpinia officinalis is still relatively rare. The present invention applies the pressing method to the preparation of ginger root extract to obtain 100% ginger root stock solution, making significant progress in extracting active ingredients.

[0024] 2. The method of the present invention does not require high-temperature preparation and is safer and more environmentally friendly than other methods.

[0025] 3. The preparation method of the present invention retains more active ingredients of ginger root compared with water extraction or conventional pressing methods. Therefore, the ginger root stock solution of the present invention has the dual effects of inhibiting 5α-reductase and promoting scalp blood microcirculation.

[0026] 4. The ginger root liquid of the present invention can be applied to the formulation of scalp care products and has the effect of preventing consumers from losing their hair. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is the Zein irritation test result of the ginger root concentrate of the present invention; Figure 2 A partial picture of the press used in the present invention (pressing plate); Figure 3 The endpoint of the CAM membrane being acted on by 10% ginger root stock solution of the present invention for 5 minutes; Figure 4 To compare the average peak values ​​of testosterone in each group in the 5α-reductase activity inhibition test; Figure 5 The results of the average testosterone peak area and 5α-reductase activity inhibition rate of each group; Figure 6 This is the test result of the ginger root liquid of the present invention promoting blood microcirculation. DETAILED DESCRIPTION

[0028] To make the purpose, technical scheme and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific implementation methods and drawings. Unless otherwise specified, the reagents and materials used in the following examples are conventional commercial products.

[0029] The relevant information of the press used in the embodiment of the present invention is as follows: Experimental press (5L, 20T tonnage), customized by Zhucheng Hengbin Machinery Co., Ltd.; the press plate is used as the main pressing component to press the plant, the press plate picture is shown in Figure 2 .

[0030] Example 1 (1) Wash, dry and disinfect the fresh ginger.

[0031] (2) Weigh 673 g of detoxified ginger, cut it into cubes, put it into a pressing bag, and place it into the material barrel of the press.

[0032] (3) Set the pressing pressure range to 10-15 MPa and continue pressing for 20 min. Take the residue for the next pressing. Repeat the pressing for 5 times and collect the liquid. A total of 477.2 g of ginger root liquid A was obtained, with a pressing rate of 70.9%.

[0033] (4) Add 1% preservative by weight (0.8% phenoxyethanol, 0.2% sodium benzoate).

[0034] (5) Add appropriate amount of citric acid to adjust the pH to 5.5.

[0035] (6) Centrifuge (3000 rpm, 30 min), add 0.1% by weight of suspending agent (0.1% xanthan gum), stir well to obtain ginger root stock solution, and measure the solid content of the ginger root stock solution to be 2.60%.

[0036] Example 2 (1) Wash, dry and disinfect the fresh Yunnan small yellow ginger.

[0037] (2) Weigh 1025 g of sterilized Yunnan small yellow ginger, cut it into cubes, put it into a pressing bag, and place it into the material barrel of the press.

[0038] (3) Set the pressing pressure range to 13-15 MPa and continue pressing for 20 min. Take the residue for the next pressing. Repeat the pressing for 5 times and collect the liquid. A total of 667 g of ginger root liquid A was obtained, with a pressing rate of 65%.

[0039] (4) Add 1.1% by weight of preservatives (0.9% phenoxyethanol, 0.12% benzoic acid, 0.08% dehydroacetic acid).

[0040] (5) Add appropriate amount of citric acid to adjust the pH to 5.5.

[0041] (6) Centrifuge (3000 rpm, 30 min), add 0.3% by weight of suspending agent (0.2% xanthan gum + 0.1% hydroxypropyl methylcellulose), stir well to obtain ginger root stock solution, and the solid content of the ginger root stock solution was measured to be 3.76%.

[0042] Test Example 1 The ginger root stock solution (Alpinia officinalis) of Example 1 was subjected to GC-MS component analysis. The analysis results are as follows.

[0043] Table 1 GC-MS analysis results of ginger root concentrate (Alpinia officinalis)

[0044] Test Example 2 The ginger root stock solution (Alpinia officinalis) of Example 1 was analyzed for amino acid composition.

[0045] 1. Related instrument information: Model: HITACHI LA8080 fully automatic amino acid analyzer; Inj. volume: 20.0.

[0046] 2. The analysis results are as follows.

[0047] Table 2 Amino acid composition of ginger root concentrate (Alpinia officinalis)

[0048] Test Example 3 Take the ginger root stock solution of Example 1 to conduct Zein irritation test, dilute the test sample with pure water to a 10% aqueous solution, make the Zein reagent and sample react completely according to the test steps, test the absorbance of the product with an ultraviolet spectrophotometer, and calculate the Zein value. The test results are shown in Figure 1 , it can be seen that ginger root liquid is non-irritating.

[0049] Test Example 4 The ginger root stock solution of Example 1 was used to prepare a solution with a mass concentration of 10%, and the mildness test was performed on the CAM membrane, with 5 minutes as the end point; a 1% SLS aqueous solution was used as a positive control; the test method was derived from the chicken embryo chorioallantoic membrane test for eye irritation / corrosion of cosmetics (SN / T2329-2009).

[0050] The eye irritation scores of CAM membranes are shown in Table 3. The end point CAM membrane pictures are shown in Figure 3 shown.

[0051] Table 3 CAM membrane eye irritation score (scoring method: IS)

[0052] The formal test results are calculated from Table 3, IS=0, which shows that the 10% ginger root stock solution is classified as non-irritating to the eyes.

[0053] Test Example 5 (5α-reductase activity inhibition test) Androgenic alopecia (AGA) is the most common type of hair loss. Its pathogenic mechanism has been widely studied. The widely accepted mechanism is that androgen (DT) is transported to hair follicles through the blood and reduced to more active DHT by 5α-reductase in hair papilla cells. DHT binds to hair papilla cell receptors and regulates the expression of genes related to hair growth, eventually leading to gradual atrophy of hair follicles and hair loss. Inhibiting the activity of 5α-reductase can inhibit the conversion of DT to DHT, thereby preventing hair loss.

[0054] 1. Test steps 1) Preparation of type II 5α-reductase: Take 10 male SD rats, fast overnight but not water, and then kill by CO2 asphyxiation. Remove the epididymis and prostate and cut them into pieces on an ice table. Use pre-cooled buffer at 0℃ to make a 1:5 homogenate in a glass homogenizer, centrifuge at 3000g for 10 min, take the supernatant and centrifuge at 10000g for 30 min, take the supernatant and dispense it into EP tubes, 1 mL per tube, which is the enzyme extract, and store in a -80℃ refrigerator.

[0055] 2) Type II 5α-reductase activity assay: The ginger root stock solution of Example 1 was used to prepare 30% (v / v) solution, 20% (v / v) solution, and 10% (v / v) solution, respectively, as sample solutions of this test example.

[0056] Weigh 0.0372 g of finasteride, add anhydrous ethanol to 1 mL, take 0.005 mL and add 4.995 mL of anhydrous ethanol as a stock solution; then take 0.005 mL of the stock solution and add 0.495 mL of anhydrous ethanol to prepare a finasteride solution with a concentration of 1 μmol / L.

[0057] The sample group contained 0.25 mL of phosphate buffer, 0.70 mL of enzyme extract, 0.1 mL of testosterone solution (0.5 mg / mL), 0.35 mL of NADPH solution (0.8 mg / mL), and 0.6 mL of sample solution (replaced with 10% ethanol solution in the blank control group and the complete reaction group, and replaced with 1 μmol / L finasteride solution in the positive group). In the blank control group, the enzyme extract was replaced with phosphate buffer, and 2.0 mL of dichloromethane was added to terminate the reaction; the complete reaction group, the positive group, and the sample group were reacted at 37°C for 30 min, and 2.0 mL of dichloromethane was added to stop the reaction after the reaction, and the reaction was stopped by shaking for 1 min and centrifuged at 5000r / min for 10 min. The upper aqueous phase was removed, and about 1.0 mL of the organic phase was removed and evaporated to dryness. The residue was dissolved in 1.0 mL of methanol and filtered with a 0.22μm filter membrane. The testosterone peak area (T) was determined by high performance liquid chromatography. Three parallel tubes were set for each reaction group.

[0058] The inhibition rate of type II 5α-reductase activity was calculated according to the following formula: Inhibition rate = (T 样品管 -T 反应管 ) / (T 空白管 -T 反应管 )*100%. Among them, T 样品管 is the sample group, T 反应管 The complete response group, T 空白管 It is the blank control group.

[0059] 2. Test results The data comparison of the average peak testosterone (T) of each group is shown in Figure 4 The results of the average testosterone peak area and 5α-reductase activity inhibition rate of each group are shown in Figure 5 . According to statistical analysis, compared with the complete reaction group, the average peak area of ​​testosterone (T) in the positive group increased, and the inhibition rate of type II 5α-reductase was 77.73%, which was statistically significant (P<0.05); the average peak area of ​​testosterone (T) in the samples increased at 30% (v / v), 20% (v / v), and 10% (v / v), and the inhibition rates of type II 5α-reductase were 77.55%, 56.53%, and 47.41%, respectively, which were statistically significant (P<0.05); the inhibition rate of type II 5α-reductase at 30% (v / v) was close to that of the positive control group (finasteride).

[0060] It can be seen that under the experimental conditions, ginger root stock solution has an anti-hair loss effect at concentrations of 30% (v / v), 20% (v / v), and 10% (v / v).

[0061] Test Example 6 (Blood Microcirculation Promotion Test) Promoting blood microcirculation in hair follicles can promote the supply of nutrients to hair follicles, promote hair follicle growth, and prevent hair loss. This test case tests the promoting effect of ginger root liquid on blood microcirculation through animal experiments. Laser speckle imaging technology is a new detection technology developed from the phenomenon that when a laser light source is irradiated on an optically rough surface of an object or a medium containing scattering substances, a laser speckle pattern containing a lot of information about the irradiated object is formed. It has the advantages of being non-invasive, non-contact, and having a large field of view. In this test case, it is used for monitoring the blood flow velocity in the biological cortex.

[0062] 1. Test steps: The experimental rats were quarantined in the quarantine room for 3 days before entering the feeding room. They were weighed before the experiment, and a normal control group and a test sample group were set up according to the weight of the animals, with 6 rats in each group.

[0063] The experimental animals were depilated on their backs one day before administration, and a 3*3 cm square area was drawn on the back with a black marker, avoiding areas with obvious blood vessels. After collecting the baseline of skin blood flow in the square area, a solvent or a test sample (the test sample group was given the test sample, i.e., the ginger root stock solution of Example 1 of the present invention, and the normal control group was given the solvent) was given in the circle and evenly applied. The skin blood flow data were collected at 15 min, 30 min, and 120 min after the test sample was given using laser speckle imaging technology. The test was repeated before and after administration on the third day (D3) of the experiment.

[0064] Statistical analysis: IBM SPSS Statistics 20 software was used for statistical analysis, and Graphprism software was used for drawing graphs.

[0065] Test results: The test results are as follows: Figure 6 As shown. Figure 6 It can be seen that before and after administration on the third day of the experiment: before administration, the blood flow velocities of the normal control group and the test sample group were 474.75±77.55 pu and 501.97±27.22 pu, respectively, and there was no significant difference between the two groups; 15 minutes after administration, the blood flow velocities of the normal control group and the test sample group were 434.05±69.72 pu and 577.65±146.95 pu, respectively, and there was a significant difference between the two groups (P<0.05), which shows that the ginger root solution of Example 1 applied to the skin of rats can increase the blood flow velocity of the skin of rats; more than 30 minutes after administration, there was no significant difference in blood flow velocity between the normal control group and the test sample group, which is speculated to be related to the metabolism or clearance of the active ingredients of the ginger root solution.

[0066] The test results show that the ginger root stock solution of the present invention has the effect of promoting skin blood microcirculation under the test conditions.

[0067] The above disclosure is only the preferred embodiment of the present invention, which certainly cannot be used to limit the scope of the present invention. Therefore, equivalent changes made according to the claims of the present invention are still within the scope of the present invention.

Claims

1. A method for preparing a ginger root stock solution for promoting scalp blood microcirculation, characterized in that: The steps include: S1. Pre-treating ginger roots to obtain ginger roots to be squeezed; S2, squeezing the ginger root to be squeezed with a squeezer to collect and obtain ginger root liquid A; S3, adding a preservative to the ginger root liquid A, adjusting the pH to obtain ginger root liquid B; S4, centrifuging the ginger root liquid B to obtain a supernatant; S5. Add a suspending agent to the upper layer liquid to obtain ginger root stock solution.

2. The method for preparing the ginger root stock solution for promoting scalp blood microcirculation according to claim 1, characterized in that: The ginger root includes one or more of Alpinia galanga and Yunnan small yellow ginger.

3. The method for preparing the ginger root stock solution for promoting scalp blood microcirculation according to claim 1, characterized in that: The suspending agent includes one or both of xanthan gum and hydroxypropyl methylcellulose.

4. The method for preparing the ginger root stock solution for promoting scalp blood microcirculation according to claim 1, characterized in that: The mass ratio of the suspending agent to the ginger root liquid A is 0.1% to 0.3%.

5. The method for preparing the ginger root stock solution for promoting scalp blood microcirculation according to claim 1, characterized in that: The press described in step S2 uses a pressing plate as a pressing component; the pressing pressure described in step S2 includes 10-15MPa; the single pressing time described in step S2 includes 20min; the number of pressings described in step S2 includes 5 times; the centrifugal speed described in step S4 includes 3000rmp / min, and the time includes 30min.

6. The method for preparing the ginger root stock solution for promoting scalp blood microcirculation according to claim 1, characterized in that: The pretreatment in step S1 includes washing, drying, disinfecting and cutting in sequence; the preservative in step S3 includes a mixture of phenoxyethanol and sodium benzoate, or a mixture of phenoxyethanol, benzoic acid and dehydroacetic acid; step S3 adjusts the pH to 5.5; step S5 includes: adding the suspending agent to the upper layer liquid, mixing evenly, and obtaining the ginger root stock solution.

7. The method for preparing the ginger root stock solution for promoting scalp blood microcirculation as claimed in claim 6, characterized in that: The slicing in step S1 includes dicing; the mass ratio of the preservative in step S3 to the ginger root liquid A in step S2 includes 1% to 1.1%; compared with the mass of the ginger root liquid A, the preservative includes a mixture of 0.8% by mass of phenoxyethanol and 0.2% by mass of sodium benzoate, or a mixture of 0.9% by mass of phenoxyethanol, 0.12% by mass of benzoic acid and 0.08% by mass of dehydroacetic acid; citric acid is used to adjust the pH in step S3; and the mixing method in step S5 includes stirring.

8. A ginger root stock solution obtained by the method for preparing the ginger root stock solution for promoting scalp blood microcirculation as claimed in claim 1, characterized in that: The solid content of the ginger root stock solution ranges from 2.60% to 3.76%.

9. An application of the ginger root liquid as claimed in claim 8, characterized in that: Used for preparing one or more of the preparations for inhibiting 5α-reductase and the preparations for promoting scalp blood microcirculation.

10. The use of the ginger root liquid as claimed in claim 9, characterized in that: It is also used to prepare scalp care products with the effect of preventing hair loss.

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