Nitrogen-fixing microbial fermentation device and method based on soybean planting soil nitrogen fixation improvement

Abstract

The invention discloses a nitrogen-fixing microbial fermentation device and method based on soybean planting soil nitrogen fixation improvement, and relates to the technical field of microbial fermentation. Linkage can be formed between the annular loading box and the multiple pumping assemblies, that is, pressure can be applied to the multiple pumping assemblies at the same time in the downward moving process of the annular loading box, and therefore the pumping assemblies can convey alcohol in the annular fuel tank into the annular loading box from multiple positions; the annular porous adsorption material can adsorb alcohol from multiple positions of the annular loading box at the same time, it is guaranteed that the whole annular porous adsorption material can be rapidly filled with alcohol, it is guaranteed that a complete fire ring can be formed after the annular porous adsorption material is ignited, and therefore the good disinfection and sterilization effect is achieved; by arranging a plurality of pumping assemblies, alcohol fuel can be quantitatively supplied to the annular porous adsorption material in real time before inoculation each time, and the situation that alcohol in the annular porous adsorption material is evaporated and cannot be ignited is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation, and in particular to a nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean-planting soil. Background Art

[0002] Nitrogen-fixing microorganisms are those that can convert atmospheric nitrogen (N 2 ) is converted into ammonia nitrogen (NH 3 ) that, through autotrophic or symbiotic association with plants, convert atmospheric nitrogen into nitrogen that can be absorbed and utilized by plants.

[0003] Among them, flame inoculation is often used to inject the bacterial solution into the fermentation tank during the nitrogen-fixing microbial fermentation process. Flame inoculation is a sterile inoculation technology. The core is to use the high temperature of the flame to kill the miscellaneous bacteria on the inoculation port of the fermentation tank and the inoculation tools, ensuring the sterile operation of the inoculation process, thereby ensuring the smooth progress of the fermentation process.

[0004] A bacterial strain inoculation device for fermentation disclosed in a patent application with reference publication number CN218089577U includes a fermentation tank body; it also includes: an interface seat, the interface seat is fixed on the fermentation tank body, an inoculation port is provided on the interface seat, an outer wall of the inoculation port is provided with an external thread, and an annular groove I is provided around the inoculation port on the interface seat; a sealing cover, the sealing cover includes a sealing cover body connected to the inoculation port, and a handle rod fixed to the sealing cover body, the sealing cover body is provided with an internal thread connected to the external thread. This patent application provides a fire extinguishing cover, which can simply and conveniently extinguish the flame after the bacterial strain inoculation is completed, thereby improving the convenience and safety of operation.

[0005] The above-mentioned vaccination device in the prior art has the following defects in actual use: 1. In order to facilitate the pouring of strains, the inoculation port on the existing microbial fermentation device is generally set at a certain angle to the fermentation tank body, that is, it is set in an inclined state. In this case, alcohol or cotton adsorbed with alcohol is placed in the annular alcohol lamp. Under the action of gravity, the alcohol will be concentrated on the lower side, and no flame will be generated at the position where alcohol is lacking, so that a circular flame protection circle cannot be formed at the inoculation port, resulting in easy contamination of miscellaneous bacteria during the inoculation process; 2. The alcohol is located inside the annular alcohol lamp, and it is difficult to quickly ignite the alcohol through the fire hole. After the inoculation operation is completed, the alcohol stored in the annular alcohol lamp is easy to evaporate when idle for a long time, resulting in a waste of alcohol fuel.

[0006] Therefore, the present invention proposes a nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean planting soil to solve the above problems. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention provides a nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean planting soil, which solves the problem that when the inoculation port on the existing microbial fermentation device is in an inclined setting state, the alcohol in the annular alcohol lamp or the cotton adsorbed with alcohol will be concentrated on the lower side under the action of gravity, and no flame will be generated at the position where alcohol is lacking, so that an annular flame protection circle cannot be formed at the inoculation port, resulting in the situation that bacteria contamination is easy to occur during the inoculation process, and it is difficult to quickly ignite alcohol through the fire outlet. After the inoculation operation is completed, the alcohol stored in the annular alcohol lamp is easy to volatilize when idle for a long time, which easily causes the problem of alcohol fuel waste.

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil, comprising a fermentation tank and an inoculation nozzle fixedly arranged on one side of the top of the fermentation tank for adding bacteria, the top of the inoculation nozzle is also detachably provided with a sealing cover for sealing, and also includes: The installation groove is opened on one side of the top of the fermentation tank, and the inoculation nozzle is arranged at the center position of the bottom of the installation groove; The flame sterilization mechanism is arranged inside the installation groove and is used to ignite before adding bacteria to form a ring of fire around the inoculation nozzle, and use the high temperature of the flame to disinfect and sterilize; The flame sterilization mechanism also includes a fuel storage assembly for storing disinfection fuel, an annular fire ring forming assembly for burning alcohol is arranged above the fuel storage assembly, and a pumping assembly, an automatic ignition unit and an ignition control assembly are also arranged between the fuel storage assembly and the annular fire ring forming assembly. The pumping assembly is used to transport the alcohol in the fuel storage assembly to the annular fire ring forming assembly, and the automatic ignition unit is used to ignite the alcohol entering the annular fire ring forming assembly.

[0009] Furthermore, the fuel storage assembly includes an annular fuel tank detachably arranged at the bottom of the mounting groove, a feeding pipe connected to the interior of the annular fuel tank is fixedly arranged on the side wall of the annular fuel tank, the top end of the feeding pipe passes through the fermentation tank and extends to the outside, and a sealing plug is detachably arranged at the top end of the feeding pipe.

[0010] Further, the annular fire ring forming assembly comprises an upper cover unit and a lower cover unit movably arranged at the bottom of the upper cover unit, and a fuel adsorption unit is arranged between the upper cover unit and the lower cover unit; The upper cover unit comprises a fire extinguishing cover which is detachably arranged in the installation groove, the fire extinguishing cover is arranged around the outer wall of the inoculation nozzle, and a plurality of avoidance through holes are evenly opened on the top of the fire extinguishing cover.

[0011] Furthermore, the lower cover unit includes an annular loading box sealed at the bottom of the fire extinguishing cover, a plurality of blocking blocks are evenly and fixedly arranged at the bottom of the inner cavity of the annular loading box, and a plurality of mounting holes for providing mounting positions for the automatic ignition unit are evenly opened at the bottom of the annular loading box; The fuel adsorption unit includes an annular porous adsorption material detachably arranged inside the annular loading box, and the top of the annular porous adsorption material is evenly provided with avoidance hole 1 and avoidance hole 2 corresponding to the position of the blocking block, and the top of the annular porous adsorption material is also provided with multiple ignition avoidance holes corresponding to the position of the installation holes.

[0012] Furthermore, the automatic ignition unit includes a piezoelectric igniter fixedly arranged in a mounting hole at the bottom of the annular loading box, and a top block is fixedly arranged on the top of the annular fuel tank and directly below the piezoelectric igniter for pushing the piezoelectric igniter to perform ignition.

[0013] Furthermore, the pumping assembly includes a cylinder body detachably arranged on the top of the annular fuel tank, a piston is sealingly and slidably arranged inside the cylinder body, a piston rod is fixedly arranged on the top of the piston, the top end of the piston rod slides through the cylinder body and extends to the outside, and a spring is sleeved on the outer wall of the piston rod and located above the cylinder body, a suction pipe connected to the interior of the cylinder body is fixedly arranged at the bottom of the cylinder body, and a one-way valve for only allowing fuel to flow into the cylinder body is fixedly arranged inside the suction pipe.

[0014] Furthermore, a high-temperature resistant hose connected to the interior of the cylinder is provided on the side wall of the cylinder, and a one-way valve 2 is fixedly provided inside the high-temperature resistant hose for only allowing fuel to flow out of the cylinder, and a micro flame arrester for preventing backfire is also fixedly provided at one end of the high-temperature resistant hose away from the cylinder, and the top of the micro flame arrester is detachably provided at the bottom of the annular fire ring forming assembly, and a metal nozzle is also fixedly provided at the output end of the micro flame arrester, which penetrates the annular fire ring forming assembly and extends into the interior thereof.

[0015] Further, the ignition control assembly includes a bearing plate fixedly arranged on the top of the annular fuel tank and a lifting plate slidably arranged on the side wall of the bearing plate, a connecting plate is fixedly arranged between the lifting plate and the bottom of the annular loading box, a movable groove is opened on the side wall of the lifting plate, a locking pin unit for locking the position of the lifting plate is arranged inside the movable groove, and a driving unit for controlling the locking pin unit to perform a locking or unlocking action is also arranged inside the movable groove; The locking pin unit includes a driving block slidably arranged inside the movable groove and an inclined groove opened on the outer wall of the driving block. A limit pin is also fixedly arranged on the side wall of the driving block, and a spring 2 is slidably sleeved on the outer wall of the limit pin and located between the driving block and the side wall of the lifting plate. The limit pin slides through the inner wall of the movable groove and extends to the outside. A plurality of limit teeth for cooperating with the limit pin to complete the locking operation are also fixedly arranged on the side wall of the bearing plate.

[0016] Furthermore, the driving unit includes a slider slidably arranged in the inclined groove and a push-pull rod fixedly arranged on the side wall of the slider, the top end of the push-pull rod slides through the lifting plate and the fermentation tank and extends to the outside, and a pull ring is also fixedly arranged on the top end of the push-pull rod.

[0017] The present invention also discloses a nitrogen-fixing microbial fermentation method based on improving nitrogen fixation in soybean-growing soil, and a nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean-growing soil. The method comprises the following steps: Step 1, pre-culturing the selected nitrogen-fixing bacteria strain in a culture medium to enable it to reach an adaptive culture stage to ensure its nitrogen fixation efficiency during the fermentation process; Step 2: before inoculation, manually release the position limit of the annular fire ring forming component by the ignition control component, and use the ignition control component to push the annular fire ring forming component downward. During this process, the pumping component transports the fuel in the fuel storage component into the annular fire ring forming component; Step 3, the automatic ignition unit generates an electric spark after being pressurized, and the fuel in the annular fire ring forming component is ignited by the electric spark to form an annular fire ring around the inoculation nozzle; Step 4: Add the pre-cultured nitrogen-fixing bacteria strain and the culture medium mixture into the fermentation tank through the inoculation nozzle for fermentation treatment.

[0018] The present invention provides a nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean planting soil. Compared with the prior art, it has the following beneficial effects: 1. A nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean planting soil, by setting a flame sterilization mechanism, can control the separation of the annular loading box and the fire extinguishing cover by pressing the pull ring before each inoculation, so that the annular porous adsorption material is exposed to the outside, laying the foundation for subsequent ignition; and the annular loading box and multiple pumping components can form a linkage, that is, during the downward movement of the annular loading box, pressure can be applied to multiple pumping components at the same time, so that the pumping components can transport alcohol in the annular fuel tank from multiple positions into the annular loading box, so that the annular porous adsorption material can absorb alcohol from multiple positions of the annular loading box at the same time, thereby ensuring that the entire annular porous adsorption material can be quickly filled with alcohol, ensuring that the annular porous adsorption material can form a complete fire circle after being ignited, so as to achieve a good disinfection and sterilization effect.

[0019] 2. A nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean-growing soil can, through the arrangement of multiple pumping components, supply alcohol fuel to the annular porous adsorption material in real time and quantitatively before each inoculation, so that the annular porous adsorption material is filled with sufficient alcohol each time it is ignited, thereby ensuring that the ignition operation can be carried out smoothly and avoiding the situation where the annular porous adsorption material cannot be ignited due to evaporation of the alcohol inside after being idle for a long time. Moreover, the method of immediate alcohol replenishment and immediate ignition can maximize the use of alcohol and avoid the problem of alcohol evaporation and waste due to long-term storage. The other spare alcohol is stored separately in the annular fuel tank, which can minimize the volatilization of alcohol.

[0020] 3. A nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean-growing soil, by setting an automatic ignition unit, can use the interaction between the piezoelectric igniter and the top block to ignite the annular porous adsorption material with the generated electric spark after the pumping unit transports alcohol into the annular porous adsorption material, thereby achieving the effect of automatic ignition immediately after automatic fuel replenishment, so that the ignition operation is not only highly automated, but also significantly simplifies the operating steps of setting the fire ring during flame inoculation, greatly reducing the difficulty of setting the fire ring.

[0021] 4. A nitrogen-fixing microbial fermentation device and method based on improving nitrogen fixation in soybean planting soil. By setting an ignition control component, it can control the lifting process of the annular loading box, and can lock the position after the annular loading box reaches a preset position, ensuring that the annular loading box and the fire extinguishing cover are in a continuously separated state, so that the flame can be transmitted upward through the space between the annular loading box and the fire extinguishing cover. After the inoculation is completed, the ignition control component can unlock the position of the annular loading box and restore it to its original position again, so that the space between the fire extinguishing cover and the annular loading box forms a closed space, which can not only have a fire extinguishing effect, but also can seal incompletely burned alcohol in a closed space to avoid alcohol volatilization, so that it can be used in the next inoculation operation; and during the upward movement of the annular loading box, the pumping component can quantitatively draw part of the alcohol from the annular fuel tank again, preparing for the rapid replenishment of alcohol into the annular porous adsorption material during the next inoculation.

[0022] Other advantages, objectives and features of the present invention will be described in the following description to some extent, and to some extent, will be obvious to those skilled in the art based on the following examination and study, or can be taught from the practice of the present invention. The objectives and other advantages of the present invention can be realized and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1It is a schematic diagram of the first overall three-dimensional structure of the present invention; Figure 2 This is a schematic diagram of the structure of the installation groove and the inoculation nozzle assembly state of the present invention; Figure 3 For the present invention Figure 1 A schematic diagram of the enlarged structure of part A; Figure 4 It is a schematic diagram of the second overall three-dimensional structure of the present invention; Figure 5 For the present invention Figure 4 A schematic diagram of the enlarged structure of part B in FIG. Figure 6 This is a schematic diagram of the structure of the flame sterilization mechanism of the present invention in the first state; Figure 7 It is a schematic diagram of the structure of the flame sterilization mechanism of the present invention in the second state; Figure 8 For the present invention Figure 7 A schematic diagram of the enlarged structure of part C in FIG. Fig. 9 This is a schematic diagram of the structure of the flame sterilization mechanism of the present invention in the first decomposition state; Fig.10 This is a schematic diagram of the structure of the flame sterilization mechanism of the present invention in the second decomposition state; Fig.11 For the present invention Fig.10 The D part in the figure is an enlarged structural diagram; Fig.12 It is a schematic cross-sectional view of the pumping assembly of the present invention; Fig.13 It is a schematic diagram of the overall structure of the pumping assembly of the present invention; Fig.14 It is a schematic structural diagram of the piezoelectric igniter and the top block in the assembled state of the present invention; Fig.15 This is a schematic diagram of the structure of the ignition control assembly in the first state of the present invention; Fig.16 For the present invention Fig.15 The enlarged structural diagram of part E in FIG. Fig.17 This is a schematic diagram of the structure of the ignition control assembly in the second state of the present invention; Fig.18 For the present invention Fig.17 The F part in the figure is an enlarged structural diagram.

[0024] In the figure: 1, fermentation tank; 2, inoculation nozzle; 3, sealing cover; 4, flame sterilization mechanism; 41, annular fuel tank; 42, feeding pipe; 43, fire extinguishing cover; 44, avoidance hole 1; 45, annular loading box; 46, blocking block; 47, annular porous adsorption material; 48, avoidance hole 2; 49, pumping assembly; 491, cylinder; 492, piston; 493, piston rod; 494, spring 1; 495, suction pipe; 496, one-way valve one; 497, high temperature resistant hose; 498, one-way valve two; 499, miniature flame arrester; 410, piezoelectric igniter; 411, top block; 412, ignition control assembly; a1, bearing plate; a2, lifting plate; a3, connecting plate; a4, movable groove; a5, driving block; a6, inclined groove; a7, slider; a8, push-pull rod; a9, pull ring; a10, limit pin; a11, spring two; a12, limit teeth; 5, installation groove. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The present invention provides three technical solutions: a nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil, specifically including the following embodiments: like Figure 1-Figure 11 , Fig.14 The first embodiment is shown: a nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil, comprising a fermentation tank 1 and an inoculation nozzle 2 fixedly arranged on one side of the top of the fermentation tank 1 for adding bacteria, and a sealing cover 3 for sealing is detachably arranged on the top of the inoculation nozzle 2, and further comprising: The installation groove 5 is opened on one side of the top of the fermentation tank 1, and the inoculation nozzle 2 is arranged at the center position of the bottom of the installation groove 5; The flame sterilization mechanism 4 is arranged inside the mounting groove 5 and is used to ignite before adding the strain to form an annular fire ring around the inoculation nozzle 2, and to perform disinfection and sterilization by utilizing the high temperature of the flame; The flame sterilization mechanism 4 also includes a fuel storage assembly for storing disinfection fuel, and an annular fire ring forming assembly for burning alcohol is arranged above the fuel storage assembly, and a pumping assembly 49, an automatic ignition unit and an ignition control assembly 412 are also arranged between the fuel storage assembly and the annular fire ring forming assembly. The pumping assembly 49 is used to transport the alcohol in the fuel storage assembly to the annular fire ring forming assembly, and the automatic ignition unit is used to ignite the alcohol entering the annular fire ring forming assembly.

[0027] The fuel storage assembly includes an annular fuel tank 41 detachably arranged at the bottom of the mounting groove 5, a feeding pipe 42 connected to the inside of the annular fuel tank 41 is fixedly arranged on the side wall, the top end of the feeding pipe 42 passes through the fermentation tank 1 and extends to the outside, and a sealing plug is detachably arranged at the top end of the feeding pipe 42. The annular fire ring forming assembly includes an upper cover unit and a lower cover unit movably arranged at the bottom of the upper cover unit, and a fuel adsorption unit is arranged between the upper cover unit and the lower cover unit; the upper cover unit includes a fire extinguishing cover 43 detachably arranged in the mounting groove 5, the fire extinguishing cover 43 is arranged around the outer wall of the inoculation nozzle 2, and a plurality of avoidance through holes 44 are evenly opened at the top of the fire extinguishing cover 43. The lower cover unit includes an annular loading box 45 sealed at the bottom of the fire extinguishing cover 43, a plurality of blocking blocks 46 are evenly fixedly arranged at the bottom of the inner cavity of the annular loading box 45, and a plurality of mounting holes for providing an installation position for the automatic ignition unit are evenly opened at the bottom of the annular loading box 45; the fuel adsorption unit includes an annular porous adsorption material 47 detachably arranged inside the annular loading box 45, and the top of the annular porous adsorption material 47 is evenly opened with avoidance through hole 1 44 and avoidance through hole 2 48 corresponding to the position of the blocking block 46, and the top of the annular porous adsorption material 47 is also opened with a plurality of ignition avoidance through holes corresponding to the positions of the mounting holes. The positions of the avoidance through hole 1 44 and the blocking block 46 are set in a one-to-one correspondence, and the structures of the blocking block 46 and the avoidance through hole 1 44 are adapted, that is, when the bottom of the fire extinguishing cover 43 and the top of the annular loading box 45 are tightly fitted, the blocking block 46 can enter the avoidance through hole 1 44 at the corresponding position to form a seal for the avoidance through hole 1 44, and when the annular porous adsorption material 47 is installed in the annular loading box 45, the avoidance through hole 2 48 can be sleeved on the outer wall of the blocking block 46 at the corresponding position. The automatic ignition unit includes a piezoelectric igniter 410 fixedly set in the mounting hole at the bottom of the annular loading box 45, and a top block 411 is fixedly set at the top of the annular fuel tank 41 and directly below the piezoelectric igniter 410, which is used to push the piezoelectric igniter 410 to perform an ignition action.

[0028] like Figure 12-13A second embodiment is shown, which differs from the first embodiment in that a pumping assembly 49 includes a cylinder 491 detachably arranged on the top of the annular fuel tank 41, a piston 492 is sealingly and slidably arranged inside the cylinder 491, a piston rod 493 is fixedly arranged on the top of the piston 492, the top of the piston rod 493 slides through the cylinder 491 and extends to the outside, and a spring 494 is sleeved on the outer wall of the piston rod 493 and located above the cylinder 491, a suction pipe 495 connected to the interior of the cylinder 491 is fixedly arranged at the bottom of the cylinder 491, and a one-way valve 496 for only allowing fuel to flow into the cylinder 491 is fixedly arranged inside the suction pipe 495. The top of the piston rod 493 is fixedly arranged at the bottom of the annular loading box 45, and the bottom of the suction pipe 495 is sealed and penetrates the annular fuel tank 41 and extends to the inside thereof, and one end of the high temperature resistant hose 497 is connected to the lower part of the inner cavity of the cylinder 491; the spring 1 494 and the spring 2 a11 are made of the same material, and the elastic modulus of the spring 1 494 is greater than that of the spring 2 a11, that is, relative to the spring 2 a11, a greater pressure is required to make the spring 1 494 elastically deformed; the side wall of the cylinder 491 is provided with a A high temperature resistant hose 497 is provided, and a one-way valve 498 is fixedly provided inside the high temperature resistant hose 497, which only allows the fuel to flow out of the cylinder 491. A micro flame arrester 499 is fixedly provided at one end of the high temperature resistant hose 497 away from the cylinder 491, which is used to prevent the occurrence of flashback. The top of the micro flame arrester 499 is detachably provided at the bottom of the annular fire ring forming component, and a metal nozzle is fixedly provided at the output end of the micro flame arrester 499, which penetrates the annular fire ring forming component and extends into the inside thereof. An asbestos layer is also fixedly provided on the outer wall of the high temperature resistant hose 497, which is used to protect it from high temperatures.

[0029] like Figure 15-18The third embodiment is shown, which differs from the second embodiment in that: the ignition control assembly 412 includes a carrier plate a1 fixedly arranged on the top of the annular fuel tank 41 and a lifting plate a2 slidably arranged on the side wall of the carrier plate a1, a connecting plate a3 is fixedly arranged between the lifting plate a2 and the bottom of the annular loading box 45, and a movable groove a4 is provided on the side wall of the lifting plate a2, and a locking pin unit for locking the position of the lifting plate a2 is provided inside the movable groove a4, and a locking pin unit for controlling the locking pin unit to execute locking is also provided inside the movable groove a4. or unlocking action; the locking pin unit includes a driving block a5 slidably arranged inside the movable groove a4 and an inclined groove a6 opened on the outer wall of the driving block a5, a limit pin a10 is also fixedly arranged on the side wall of the driving block a5, and a spring a11 is slidably sleeved on the outer wall of the limit pin a10 and located between the driving block a5 and the side wall of the lifting plate a2, the limit pin a10 slides through the inner wall of the movable groove a4 and extends to the outside, and a plurality of limit teeth a12 are also fixedly arranged on the side wall of the bearing plate a1 for cooperating with the limit pin a10 to complete the locking operation. That is, when the end of the limit pin a10 enters the gap between two adjacent limit teeth a12, the limit pin a10 is blocked by the limit teeth a12 and cannot move. If the driving block a5 pulls the limit pin a10 to move away from the limit teeth a12, the limit pin a10 can be disengaged from the gap between the two adjacent limit teeth a12, and the lifting plate a2 can move in the vertical direction. The driving unit includes a slider a7 slidably arranged in the inclined groove a6 and a push-pull rod a8 fixedly arranged on the side wall of the slider a7. The top end of the push-pull rod a8 slides through the lifting plate a2 and the fermentation tank 1 and extends to the outside, and a pull ring a9 is also fixedly arranged on the top end of the push-pull rod a8.

[0030] The embodiment of the present invention also provides a nitrogen-fixing microbial fermentation method based on improving nitrogen fixation in soybean planting soil, and a nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil. The method comprises the following steps: Step 1, pre-culturing the selected nitrogen-fixing bacteria strain in a culture medium to enable it to reach an adaptive culture stage to ensure its nitrogen fixation efficiency during the fermentation process; Step 2: before inoculation, manually release the position limit of the annular fire ring forming assembly by the ignition control assembly 412, and push the annular fire ring forming assembly downward with the help of the ignition control assembly 412. During this process, the pumping assembly 49 transports the fuel in the fuel storage assembly into the annular fire ring forming assembly; Step 3, the automatic ignition unit generates an electric spark after being pressurized, and the fuel in the annular fire ring forming component is ignited by the electric spark to form an annular fire ring around the inoculation nozzle 2; Step 4: inject the pre-cultured nitrogen-fixing bacteria strain and the culture medium mixture into the fermentation tank 1 through the inoculation nozzle 2 for fermentation treatment.

[0031] The specific process is as follows: in the initial state, no pressure is applied to the top of the push-pull rod a8, the end of the limit pin a10 is located in the gap between two limit teeth a12, and the position of the lifting plate a2 is in a locked state; After wearing fireproof gloves, hold the pull ring a9 and push the push-pull rod a8 downward. The slider a7 moves vertically downward under the push of the push-pull rod a8. Since the slider a7 is slidably arranged in the inclined groove a6, the driving block a5 slides in the direction away from the limiting tooth a12 under the joint action of the slider a7 and the inclined groove a6. When the limiting pin a10 synchronously drives the block a5 to move, the end of the limiting pin a10 disengages from the gap between two of the limiting teeth a12. When the pull ring a9 is continuously pushed downward, since the slider a7 has slid to the lowest point of the inclined groove a6, the driving block a5 cannot move under the thrust of the push-pull rod a8, so that the lifting plate a2 and the pull ring a9 can move downward synchronously. Since the two ends of the connecting plate a3 are connected to the lifting plate a2 and the annular loading box 45 at the same time, when the lifting plate a2 moves downward along the inner wall of the carrying plate a1, the annular loading box 45 is synchronously driven to move downward, so that the annular loading box 45 and the fire extinguishing cover 43 are separated, and a larger gap is formed between the two. When the annular loading box 45 moves downward, the top end of the piston rod 493 receives the downward pressure from the annular loading box 45 and pushes the piston 492 to move downward along the inner wall of the cylinder 491. The alcohol fuel in the cylinder 491 flows into the inner cavity of the annular loading box 45 through the high temperature resistant hose 497, the micro flame arrester 499 and the metal nozzle. Since the annular porous adsorption material 47 has strong adsorption, the alcohol fuel entering the annular loading box 45 is adsorbed by the annular porous adsorption material 47. When the annular loading box 45 continues to move downward, the bottom end of the piezoelectric igniter 410 contacts the top of the top block 411, and the top block 411 applies a squeezing force to the bottom end of the piezoelectric igniter 410. An electric spark is formed at the end of the piezoelectric igniter 410 located inside the annular loading box 45, and the alcohol fuel adsorbed in the annular porous adsorption material 47 is quickly ignited by the electric spark. The flame quickly spreads along the outer wall of the annular porous adsorption material 47 to form an annular fire ring. At this time, the pull ring a9 is released, and the elastic force of the spring a11 pushes the limit pin a10 to quickly reset. The end of the limit pin a10 enters the gap between two of the limit teeth a12 again, so that the position of the annular loading box 45 is locked, and the flame on the surface of the annular porous adsorption material 47 burns upward through the gap between the annular loading box 45 and the fire extinguishing cover 43 and the multiple avoidance through holes 44 on the top of the fire extinguishing cover 43. Then, the sealing cover 3 is removed from the inoculation nozzle 2. After removal, the sealing cover 3 is always kept in the flame. Then, a container containing nitrogen-fixing bacteria and culture solution is held and injected into the inoculation nozzle 2. The nitrogen-fixing bacteria and culture solution enter the fermentation tank 1 through the inoculation nozzle 2 for subsequent fermentation. It should be noted that the fermentation tank 1 has been disinfected and sterilized before inoculation. After the inoculation is completed, the sealing cover 3 is covered on the inoculation mouth 2 again, and then the pull ring a9 is pressed down again to release the lock between the limit pin a10 and the limit tooth a12, and then the pull ring a9 is pulled upward to the original position. In this process, the annular loading box 45 is pulled upward synchronously by the connecting plate a3 until the top of the annular loading box 45 and the bottom of the fire extinguishing cover 43 are against each other. At this time, multiple inclined grooves a6 enter the avoidance through hole 44 at the corresponding position. Since a closed space is formed between the annular loading box 45 and the fire extinguishing cover 43, the alcohol in the annular porous adsorption material 47 cannot burn due to lack of oxygen, and the residual alcohol is in No volatilization will occur in the closed space, and when the piston rod 493 moves upward with the annular loading box 45, the cavity stroke between the bottom of the piston 492 and the cylinder 491 creates negative pressure, and the alcohol in the annular fuel tank 41 is sucked into the cylinder 491 through the suction tube 495 for storage in preparation for the next flame inoculation. It should be noted here that an air inlet is also provided on the top of the annular fuel tank 41, and a breathing valve is fixedly provided in the air inlet. When the internal pressure of the annular fuel tank 41 is higher or lower than the external atmospheric pressure, the breathing valve will automatically open or close to maintain internal pressure balance and prevent rupture or collapse due to excessively high or low pressure.

[0032] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil, comprising a fermentation tank and an inoculation nozzle fixedly arranged on one side of the top of the fermentation tank for adding bacteria, and a sealing cover for sealing is detachably arranged on the top of the inoculation nozzle, characterized in that: Also includes: The installation groove is opened on one side of the top of the fermentation tank, and the inoculation nozzle is arranged at the center position of the bottom of the installation groove; The flame sterilization mechanism is arranged inside the installation groove and is used to ignite before adding bacteria to form a ring of fire around the inoculation nozzle, and use the high temperature of the flame to disinfect and sterilize; The flame sterilization mechanism also includes a fuel storage assembly for storing disinfection fuel, an annular fire ring forming assembly for burning alcohol is arranged above the fuel storage assembly, and a pumping assembly, an automatic ignition unit and an ignition control assembly are also arranged between the fuel storage assembly and the annular fire ring forming assembly. The pumping assembly is used to transport the alcohol in the fuel storage assembly to the annular fire ring forming assembly, and the automatic ignition unit is used to ignite the alcohol entering the annular fire ring forming assembly.

2. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 1, characterized in that: The fuel storage assembly includes an annular fuel tank detachably arranged at the bottom of the mounting groove, a feeding pipe connected to the interior of the annular fuel tank is fixedly arranged on the side wall of the annular fuel tank, the top end of the feeding pipe passes through the fermentation tank and extends to the outside, and a sealing plug is detachably arranged at the top end of the feeding pipe.

3. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 2, characterized in that: The annular fire ring forming assembly comprises an upper cover unit and a lower cover unit movably arranged at the bottom of the upper cover unit, and a fuel adsorption unit is arranged between the upper cover unit and the lower cover unit; The upper cover unit comprises a fire extinguishing cover which is detachably arranged in the installation groove, the fire extinguishing cover is arranged around the outer wall of the inoculation nozzle, and a plurality of avoidance through holes are evenly opened on the top of the fire extinguishing cover.

4. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 3, characterized in that: The lower cover unit comprises an annular loading box sealedly arranged at the bottom of the fire extinguishing cover, a plurality of blocking blocks are evenly and fixedly arranged at the bottom of the inner cavity of the annular loading box, and a plurality of mounting holes for providing mounting positions for the automatic ignition unit are evenly opened at the bottom of the annular loading box; The fuel adsorption unit includes an annular porous adsorption material detachably arranged inside the annular loading box, and the top of the annular porous adsorption material is evenly provided with avoidance hole 1 and avoidance hole 2 corresponding to the position of the blocking block, and the top of the annular porous adsorption material is also provided with multiple ignition avoidance holes corresponding to the position of the installation holes.

5. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 4, characterized in that: The automatic ignition unit comprises a piezoelectric igniter fixedly arranged in a mounting hole at the bottom of the annular loading box, and a top block is fixedly arranged on the top of the annular fuel tank and directly below the piezoelectric igniter for pushing the piezoelectric igniter to perform ignition.

6. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 2, characterized in that: The pumping assembly includes a cylinder body detachably arranged on the top of the annular fuel tank, a piston is sealingly and slidably arranged inside the cylinder body, a piston rod is fixedly arranged on the top of the piston, the top end of the piston rod slides through the cylinder body and extends to the outside, and a spring is sleeved on the outer wall of the piston rod and located above the cylinder body, a suction pipe connected to the interior of the cylinder body is fixedly arranged at the bottom of the cylinder body, and a one-way valve for only allowing fuel to flow into the cylinder body is fixedly arranged inside the suction pipe.

7. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 6, characterized in that: A high-temperature resistant hose connected to the interior of the cylinder is provided on the side wall of the cylinder, and a one-way valve 2 is fixedly provided inside the high-temperature resistant hose for only allowing fuel to flow out of the cylinder, and a micro flame arrester for preventing backfire is also fixedly provided at one end of the high-temperature resistant hose away from the cylinder, and the top of the micro flame arrester is detachably provided at the bottom of the annular fire ring forming assembly, and a metal nozzle is also fixedly provided at the output end of the micro flame arrester, which penetrates the annular fire ring forming assembly and extends into the interior thereof.

8. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 1, characterized in that: The ignition control assembly comprises a bearing plate fixedly arranged on the top of the annular fuel tank and a lifting plate slidably arranged on the side wall of the bearing plate, a connecting plate is fixedly arranged between the lifting plate and the bottom of the annular loading box, a movable groove is provided on the side wall of the lifting plate, a locking pin unit for locking the position of the lifting plate is arranged inside the movable groove, and a driving unit for controlling the locking pin unit to perform locking or unlocking actions is also arranged inside the movable groove; The locking pin unit includes a driving block slidably arranged inside the movable groove and an inclined groove opened on the outer wall of the driving block. A limit pin is also fixedly arranged on the side wall of the driving block, and a spring 2 is slidably sleeved on the outer wall of the limit pin and located between the driving block and the side wall of the lifting plate. The limit pin slides through the inner wall of the movable groove and extends to the outside. A plurality of limit teeth for cooperating with the limit pin to complete the locking operation are also fixedly arranged on the side wall of the bearing plate.

9. The nitrogen-fixing microbial fermentation device based on improving nitrogen fixation in soybean planting soil according to claim 8, characterized in that: The driving unit comprises a slider slidably arranged in the inclined groove and a push-pull rod fixedly arranged on the side wall of the slider, the top end of the push-pull rod slides through the lifting plate and the fermentation tank and extends to the outside, and a pull ring is fixedly arranged on the top end of the push-pull rod.

10. A nitrogen-fixing microbial fermentation method based on improving nitrogen fixation in soybean planting soil, characterized in that: The nitrogen-fixing microbial fermentation device for improving nitrogen fixation in soybean planting soil as claimed in any one of claims 1 to 9 comprises the following steps: Step 1: pre-cultivating the selected nitrogen-fixing bacteria strain in a culture medium to allow it to reach an adaptive culture stage and ensure its nitrogen fixation efficiency during the fermentation process; Step 2: before inoculation, manually release the position limit of the annular fire ring forming component by the ignition control component, and use the ignition control component to push the annular fire ring forming component downward. During this process, the pumping component transports the fuel in the fuel storage component into the annular fire ring forming component; Step 3, the automatic ignition unit generates an electric spark after being pressurized, and the fuel in the annular fire ring forming component is ignited by the electric spark to form an annular fire ring around the inoculation nozzle; Step 4: Add the pre-cultured nitrogen-fixing bacteria strain and the culture medium mixture into the fermentation tank through the inoculation nozzle for fermentation treatment.

Images