Use method of allantoin for relieving waterlogging disaster of corn

Abstract

The invention discloses a use method of allantoin in relieving corn waterlogging and waterlogging disasters, and belongs to the technical field of corn planting. The concentration of the allantoin is 5mM. L <-1 >. The using method of the allantoin comprises the following steps that firstly, compound fertilizer is applied in soil, and in the compound fertilizer, the ratio of nitrogen fertilizer to phosphate fertilizer to potash fertilizer is 15: 15: 15; step 2, sowing and watering in the fertilized soil; 3, continuously spraying allantoin on leaf surfaces for 3 days when the seedlings grow to a three-leaf and one-core period, and adding two drops of Tween-20 as an adhesive before spraying. Therefore, by adopting the application method of the allantoin in relieving the waterlogging disaster of the corn, the technical problem that the growth and development of the corn in the waterlogging disaster are affected in the prior art is relieved.

Description

Technical Field

[0001] The present application relates to the technical field of corn planting, and in particular to a method for using allantoin in alleviating corn waterlogging disasters. Background Art

[0002] As an important grain production base in my country, the Huanghuaihai region is one of the core production areas of summer corn in my country, and plays an irreplaceable role in ensuring national food security. The corn seedling stage coincides with the concentrated precipitation period in summer, and is easily harmed by flooding, which seriously affects the growth and development of corn, resulting in varying degrees of reduction in yield (Qi and Pan 2022; Ren et al. 2020). At present, waterlogging disasters have become an important limiting factor affecting my country's food security. Therefore, taking appropriate cultivation measures is the key to alleviating the impact of waterlogging stress on crop growth and yield, and is also one of the important ways to ensure agricultural resistance and stable production. Summary of the invention

[0003] The purpose of the present application is to provide a method for using allantoin in alleviating waterlogging disasters in corn, thereby alleviating the technical problem in the prior art that the growth and development of corn is affected by waterlogging disasters.

[0004] The present application provides a method for using allantoin in alleviating corn waterlogging disasters, wherein the concentration of allantoin is 5 mM·L -1 .

[0005] Preferably, the method for using allantoin comprises the following steps: Step 1: Apply compound fertilizer to the soil. The ratio of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer in the compound fertilizer is 15:15:15; Step 2: Sow seeds in the fertilized soil and water; Step 3: When the seedlings grow to the 3-leaf 1-heart stage, spray allantoin on the leaves continuously for 3 days. Add 2 drops of Tween-20 as an adhesive before spraying.

[0006] Preferably, the nitrogen fertilizer in step 1 is one or more of urea, ammonium chloride and ammonium sulfate.

[0007] Preferably, the phosphate fertilizer is selected from one or more of superphosphate and calcium magnesium phosphate.

[0008] Preferably, the amount of compound fertilizer applied is 1 kg per mu of land.

[0009] Preferably, the sowing depth in step 2 is 3 cm.

[0010] Therefore, the present application provides a method for using allantoin in alleviating corn waterlogging disasters, which has the following beneficial effects: 1. Spraying corn seedlings under waterlogging stress with a reasonable concentration of allantoin improved the height of corn plants and the morphological structure of the root system, and promoted the accumulation of dry matter in the plants; 2. Allantoin spraying method is simple and the cost is low; 3. High efficiency, reduces the amount of pesticides used, causes less pollution to the environment, and is safe for users. DETAILED DESCRIPTION

[0011] The technical solution of the present invention is further illustrated by the following examples.

[0012] Example 1 The corn variety selected by the present invention is Zhengdan 958, which is one of the waterlogging-resistant corn varieties with the largest planting area in the Huanghuaihai region.

[0013] The experiment was conducted in the Agricultural Garden of Anhui Agricultural University from April to October 2024. The soil used in the experiment was taken from the 0-20cm tillage layer of the previous crop sorghum field in the National High-tech Industrial Park of Anhui Agricultural University. The test soil was brown soil with an organic matter content of 13.07g kg -1 、Total nitrogen 1.03g·kg -1 , alkaline nitrogen 64.64g·kg -1 , fast-acting phosphorus 18.25 mg kg -1 , fast-acting potassium 203.51 mg·kg -1 The allantoin (ALN) used in the experiment was purchased from Nanjing Dulai Biotechnology Co., Ltd. with a purity of 98.5%.

[0014] A pot experiment was conducted. The pot specifications were as follows: pot height 17 cm, diameter 21 cm, bottom diameter 15 cm, pot bottom with holes, and the number of pots was 120. The soil was naturally dried, crushed and sieved, and compound fertilizer (N:P:K=15:15:15) was added and mixed well. Each pot was filled with 3.5 ± 0.05 kg of soil. Each plot was placed in a random block with a row spacing of 60 cm and a plant spacing of 20 cm. Five seeds were sown in each pot, the sowing depth was 3 cm and watered thoroughly, and watering was continued until flooding. Thinning began when the seedlings grew to 3 leaves and 1 heart, leaving 96 pots with stable root systems and consistent growth. The experiment was carried out after grouping. Foliar spraying was carried out at 8:00 in the morning during the three-leaf and one-heart period, and spraying was continued for 3 days. Three treatments were set up: normal soil moisture treatment + foliar spraying of distilled water (CK); flooding treatment + foliar spraying of distilled water (W); flooding treatment + foliar spraying of 5 mM·L -1Allantoin (ALN5); When spraying, the leaves should be wet, and water droplets should accumulate on the front and back sides without dripping. Add 2 drops of Tween-20 as an adhesive before spraying. The day after the third spraying, the test potted plants were placed in a non-porous cultivation pot with a height of 21 cm, a diameter of 28 cm, and a bottom diameter of 23 cm. Watering treatment began at 8:00 in the morning. The water level was 3-4 cm higher than the soil layer. The water was flooded for 3 to 6 days. If the water temperature in the pot was too high, it was adjusted by adding cold water to maintain a suitable normal temperature.

[0015] After the corn seedlings were flooded for 3 and 6 days, the plant height, root length, root surface area, root volume, and dry matter accumulation of each part of the corn were measured in each treatment group. Five corn seedlings were randomly selected for each treatment to measure plant height, root morphology, and dry weight. The plant height was measured from the base of the rhizome to the top of the longest leaf with a ruler. The seed roots and adventitious roots of the corn seedlings were scanned using an MRS-3200A3L A3 color platform scanner. Dry weight: Fresh samples of stems, leaves, and roots were placed in an oven at 105°C for 30 minutes, and then dried at 80°C and weighed.

[0016] Table 1 Effects of exogenous allantoin on the height of maize seedlings under flooding stress

[0017] As shown in Table 1, flooding stress at the seedling stage of maize can significantly reduce the height of the seedlings, while the application of allantoin can significantly resist the effect of flooding on the height of maize. -1 Allantoin treatment could restore the height of maize to 97.03% and 95.97% on the 3rd and 6th day, respectively, which could alleviate the effect of flooding stress on maize height at the seedling stage.

[0018] Table 2 Effects of exogenous allantoin on root length of maize seedlings under flooding stress

[0019] As shown in Table 2, flooding stress at the seedling stage of corn can significantly reduce the root length of seedlings, while the application of allantoin can significantly resist the effect of flooding on the root growth of corn seedlings. -1 Allantoin treatment could restore the length of seminal roots and adventitious roots of maize to 85.79% and 59.27% ​​respectively on the 6th day, which could alleviate the effect of flooding stress on the root length of maize at the seedling stage.

[0020] Table 3 Effects of exogenous allantoin on root surface area of ​​maize seedlings under flooding stress

[0021] As shown in Table 3, flooding stress at the seedling stage of corn can significantly reduce the root surface area of ​​seedlings, while the application of allantoin can significantly resist the effect of flooding on the root growth of corn seedlings. -1 Allantoin treatment could restore the surface area of ​​maize seminal roots and adventitious roots to 87.79% and 80.07% respectively on the 6th day, which could alleviate the effect of flooding stress on the root surface area of ​​maize at the seedling stage.

[0022] Table 4 Effects of exogenous allantoin on root volume of maize seedlings under flooding stress

[0023] As shown in Table 4, flooding stress at the seedling stage of corn can significantly reduce the root volume of seedlings, while the application of allantoin can significantly resist the effect of flooding on the root growth of corn seedlings. -1 Allantoin treatment could restore the volume of seminal roots and adventitious roots of maize to 90.60% and 95.88% respectively on the 6th day, which could alleviate the effect of flooding stress on the root volume of maize at the seedling stage.

[0024] Table 5 Effects of exogenous allantoin on the average root diameter of corn seedlings under flooding stress

[0025] As shown in Table 5, flooding stress at the seedling stage of corn can significantly reduce the average root diameter of seedlings, while the application of allantoin can significantly resist the effect of flooding on the root growth of corn seedlings. -1 Allantoin treatment could restore the average root diameter of maize seminal roots and adventitious roots to 85.00% and 77.56% respectively on the 6th day, which could alleviate the effect of flooding stress on the average root diameter of maize at the seedling stage.

[0026] Table 6 Effects of exogenous allantoin on dry matter accumulation of maize seedlings under flooding stress

[0027] As shown in Table 6, flooding stress at the seedling stage of corn can significantly reduce the dry matter accumulation of seedlings, while the application of allantoin can significantly resist the effect of flooding on the growth of corn seedlings. -1 Allantoin treatment could restore the dry matter accumulation of maize seminal roots, adventitious roots and aboveground parts to 87.79%, 97.08% and 73.42% on the 6th day, respectively, which could alleviate the effect of flooding stress on the dry matter accumulation of maize at the seedling stage.

[0028] Therefore, the present application provides a method for using allantoin in alleviating corn waterlogging disasters, thereby alleviating the technical problem in the prior art that the growth and development of corn is affected by waterlogging disasters.

[0029] In the description of this specification, the description with reference to the terms "an experimental example", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the experimental example or example are included in at least one experimental example or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same experimental example or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more experimental examples or examples in a suitable manner.

[0030] Finally, it should be noted that the above experimental examples are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred experimental examples, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for using allantoin in alleviating corn waterlogging disasters, characterized in that: The concentration of allantoin is 5mM·L -1 .

2. The method for using allantoin in alleviating corn waterlogging disaster according to claim 1, characterized in that: The method of using allantoin includes the following steps: Step 1: Apply compound fertilizer to the soil. The ratio of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer in the compound fertilizer is 15:15:15; Step 2: sow seeds in the fertilized soil and water them; Step 3: When the seedlings grow to the 3-leaf 1-heart stage, spray allantoin on the leaves continuously for 3 days. Add 2 drops of Tween-20 as an adhesive before spraying.

3. The method for using allantoin in alleviating corn waterlogging disaster according to claim 1, characterized in that: The nitrogen fertilizer in step 1 is one or more of urea, ammonium chloride and ammonium sulfate.

4. The method for using allantoin in alleviating corn waterlogging disaster according to claim 1, characterized in that: The phosphate fertilizer can be one or more of superphosphate and calcium magnesium phosphate.

5. The method for using allantoin in alleviating corn waterlogging disaster according to claim 1, characterized in that: The application rate of compound fertilizer is 1 kg per mu of land.

6. The method for using allantoin in alleviating corn waterlogging disaster according to claim 1, characterized in that: The sowing depth in step 2 is 3 cm.

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