Highland barley dietary fiber and preparation method thereof

Abstract

The invention relates to the technical field of food production, in particular to highland barley dietary fiber and a preparation method thereof.The preparation method comprises the steps that highland barley is subjected to steam explosion, drying, grinding and screening in sequence to obtain a highland barley material, and when it is judged that preparation of the highland barley material does not meet a preset standard according to an explosion characteristic value, the highland barley dietary fiber is obtained; secondarily judging whether the preparation of the highland barley material meets the preset standard or increasing the steam explosion pressure of the steam explosion machine; preparing a highland barley suspension from a highland barley material meeting a preset standard, sequentially adding a high-temperature-resistant alpha-amylase solution, an alkaline protease solution and a saccharifying enzyme into the highland barley suspension to obtain a highland barley saccharification liquid, and judging whether the preparation of the highland barley saccharification liquid meets the preset standard or not according to the enzymolysis characteristic value; the ethanol solution is added into the highland barley saccharified liquid meeting the preset standard for centrifugal operation, the obtained precipitate is the highland barley dietary fiber, and whether the preparation of the highland barley dietary fiber meets the preset standard or not is judged according to the light absorption characteristic value, so that the quality of the highland barley dietary fiber is improved.

Description

Technical Field

[0001] The invention relates to the technical field of food production, and in particular to highland barley dietary fiber and a preparation method thereof. Background Art

[0002] Highland barley is a high-fiber grain with a rich dietary fiber content and various types. It has a variety of health benefits and has broad application prospects in the fields of food and health products. Highland barley is rich in vitamin B and vitamin E, as well as minerals such as potassium, magnesium, phosphorus, and calcium, which help improve immunity and prevent osteoporosis. The dietary fiber in highland barley can promote digestion, relieve constipation, lower blood sugar and cholesterol, and help maintain heart health. By incorporating these nutrients into food, you can make highland barley flour, highland barley wine, highland barley soy sauce, highland barley vinegar, highland barley porridge, highland barley steamed buns, highland barley biscuits, highland barley beer, and highland barley oatmeal tea, making the food not only delicious but also healthy.

[0003] Highland barley dietary fiber includes soluble dietary fiber and insoluble dietary fiber. Soluble dietary fiber can slow down the digestion and absorption of food, reduce the rate of blood sugar rise, and is beneficial to diabetic patients. In addition, it can combine with cholesterol and promote its excretion from the body, thereby lowering the cholesterol level in the blood; while insoluble dietary fiber can only promote intestinal peristalsis and prevent constipation; therefore, soluble dietary fiber is more functional. However, in highland barley, the proportion of soluble dietary fiber with stronger functionality is extremely low.

[0004] Chinese Patent Publication No.: CN104382020A discloses a method for preparing a highland barley dietary fiber-polyphenol complex, which is specifically as follows: dissolving highland barley flour in a buffer solution, performing enzymolysis in the dark, centrifuging the enzymolysis solution while hot, and precipitating the insoluble dietary fiber-polyphenol complex (IDF-PC) in highland barley, freezing the solution at low temperature after collection, freeze-drying the solution under vacuum, and storing the solution at -20°C; adding ethanol to the supernatant after centrifugation, allowing the solution to cool naturally to room temperature, and precipitating the solution under low temperature overnight, taking the lower turbid part and centrifuging the solution, and precipitating the soluble dietary fiber-polyphenol complex (SDF-PC) in highland barley, freezing the solution at low temperature after collection, freeze-drying the solution under vacuum, and storing the solution at -20°C. It can be seen that although the above technical scheme involves enzymatic hydrolysis of highland barley and centrifugal acquisition of highland barley dietary fiber, there is a lack of steam explosion of highland barley before enzymatic hydrolysis, a lack of detection of whether the enzymatic hydrolysis is sufficient after the enzymatic hydrolysis reaction, and no absorbance detection of the highland barley dietary fiber, which leads to the problems of low preparation efficiency of highland barley dietary fiber and poor quality of highland barley dietary fiber. Summary of the invention

[0005] To this end, the present invention provides a highland barley dietary fiber and a preparation method thereof, so as to overcome the problems of low preparation efficiency and poor quality of the highland barley dietary fiber in the prior art.

[0006] To achieve the above object, on the one hand, the present invention provides a method for preparing highland barley dietary fiber, comprising: Sequentially steam-exploding, drying, grinding and screening highland barley particles to obtain highland barley material, obtaining the specific surface area and porosity of the highland barley material, and obtaining a blasting characteristic value. When it is determined that the preparation of the highland barley material does not meet the preset standard according to the blasting characteristic value, it is secondarily determined whether the preparation of the highland barley material meets the preset standard according to the pore size distribution characterization parameter of the highland barley material, or the steam explosion pressure of the steam blasting machine is increased; After making highland barley material that meets the preset standard into highland barley suspension, obtaining the initial viscosity and initial reducing sugar content of the highland barley suspension, adding a high temperature resistant α-amylase solution into the highland barley suspension, and continuously stirring with a magnetic stirrer under high temperature conditions to obtain a highland barley starch enzymolysis solution; After the highland barley starch enzymolyzate is cooled, the pH of the solution is adjusted and then an alkaline protease solution is added, and the highland barley protein enzymolyzate is obtained by continuous stirring with a magnetic stirrer under high temperature conditions; After the highland barley protein enzymolysis liquid is cooled, the pH value of the solution is adjusted and then a saccharifying enzyme is added, and a magnetic stirrer is continuously stirred under high temperature conditions to obtain a highland barley saccharification liquid, and the viscosity and reducing sugar content of the highland barley saccharification liquid are obtained to obtain an enzymolysis characteristic value, and whether the preparation of the highland barley saccharification liquid meets a preset standard is determined according to the enzymolysis characteristic value; Adding an ethanol solution to a highland barley saccharification liquid that meets a preset standard, naturally cooling to room temperature, standing for a preset time, and then centrifuging, the resulting precipitate is highland barley dietary fiber, obtaining an absorption characteristic value of the highland barley dietary fiber, and determining whether the preparation of the highland barley dietary fiber meets the preset standard based on the absorption characteristic value; The highland barley dietary fiber that meets the preset standards is dried and stored away from light.

[0007] Further, the process of determining that the preparation of the highland barley material does not meet the preset standard according to the blasting characteristic value includes: Comparing the blasting characteristic value with the first preset blasting characteristic value and the second preset blasting characteristic value respectively; If the explosion characteristic value is less than the first preset explosion characteristic value, it is determined that the preparation of the highland barley material does not meet the preset standard, and the steam explosion pressure of the steam explosion is increased according to the difference between the first preset explosion characteristic value and the explosion characteristic value; If the blasting characteristic value is greater than or equal to the second preset blasting characteristic value, it is determined that the preparation of the highland barley material does not meet the preset standard, and a secondary determination is made based on the pore size distribution characterization parameter of the highland barley material whether the preparation of the highland barley material meets the preset standard.

[0008] Furthermore, the process of obtaining the burst characteristic value includes: Weigh a highland barley sample of a preset weight, load the highland barley sample into a sample cell of a specific surface area analyzer, introduce nitrogen, record the pressure change and adsorption amount during the highland barley sample adsorption of nitrogen, perform linear fitting according to the BET equation, calculate the adsorption amount, and the specific surface area is the ratio between the adsorption amount and the preset weight; The ratio between the specific surface area and the preset specific surface area is recorded as the explosion characteristic value.

[0009] Furthermore, several pressure increasing methods are provided for increasing the steam explosion pressure of the steam explosion, and each pressure increasing method has a different increase range for the steam explosion pressure of the steam explosion.

[0010] Further, a secondary determination is made as to whether the preparation of the highland barley material meets a preset standard based on the pore size distribution characterization parameter, wherein: If the pore size distribution characterization parameter is less than a preset pore size distribution characterization threshold, it is determined that the preparation of the highland barley material meets the preset standard, and the highland barley material meeting the preset standard is made into a highland barley suspension; If the pore size distribution characterization parameter is greater than or equal to the preset pore size distribution characterization threshold, it is determined that the preparation of the highland barley material does not meet the preset standard, and the steam explosion time of the steam explosion machine is reduced according to the difference between the pore size distribution characterization parameter and the preset pore size distribution characterization threshold; The pore size distribution characterization parameter is the pore size standard deviation of highland barley material.

[0011] Further, whether the preparation of the highland barley saccharification liquid meets the preset standard is determined according to the enzymolysis characteristic value, wherein: If the enzymolysis characteristic value is less than the preset enzymolysis characteristic threshold, it is determined that the preparation of the highland barley saccharification liquid does not meet the preset standard, and the stirring rate of the magnetic stirrer is synchronously increased according to the difference between the preset enzymolysis characteristic threshold and the enzymolysis characteristic value; If the enzymolysis characteristic value is greater than or equal to the preset enzymolysis characteristic threshold, it is determined that the preparation of the highland barley saccharification liquid meets the preset standard, and the highland barley saccharification liquid that meets the preset standard is centrifuged.

[0012] Furthermore, the enzymatic hydrolysis characteristic value is jointly determined by the viscosity change and the reducing sugar content change of the highland barley suspension.

[0013] Further, whether the preparation of the highland barley dietary fiber meets the preset standard is determined according to the absorbance characteristic value, wherein: If the absorbance characteristic value is less than a preset absorbance threshold value, it is determined that the preparation of the highland barley dietary fiber does not meet the preset standard, and the speed of the centrifuge is increased according to the difference between the preset absorbance threshold value and the absorbance characteristic value; If the absorbance characteristic value is greater than or equal to the preset absorbance threshold, it is determined that the preparation of the highland barley dietary fiber meets the preset standard; The absorbance characteristic value is the ratio between the absorbance of the highland barley dietary fiber and a preset absorbance.

[0014] Furthermore, the increase amplitude of the rotation speed of the centrifuge is positively correlated with the absorbance difference, wherein the absorbance difference is the difference between the preset absorbance threshold and the absorbance characteristic value.

[0015] On the other hand, the present invention also provides highland barley dietary fiber prepared according to the preparation method of highland barley dietary fiber, wherein the ratio of soluble dietary fiber to insoluble dietary fiber of the highland barley dietary fiber is 13:9.

[0016] Compared with the prior art, the beneficial effects of the present invention are that, by setting a dual judgment standard of explosion characteristic value and pore size distribution characterization parameter, the present invention can finely control the steam explosion of highland barley and improve the soluble dietary fiber content; by multi-step enzymatic hydrolysis treatment, the starch, protein and polysaccharide molecules in the highland barley are effectively removed, and the purity of the highland barley dietary fiber is improved; by introducing the enzymatic hydrolysis characteristic value, the highland barley enzymatic hydrolysis reaction process is monitored in real time, and the stirring rate of the magnetic stirrer is automatically adjusted; by introducing the light absorption characteristic value, the centrifugal process is intelligently monitored and adjusted, and the quality of the highland barley dietary fiber is improved.

[0017] Furthermore, the present invention obtains highland barley material by sequentially steam-exploding, drying, grinding and screening highland barley particles. Steam explosion effectively improves the yield of soluble dietary fiber, increases porosity, and improves the efficiency of subsequent highland barley dietary fiber preparation; drying, grinding and screening further refine the highland barley particles, thereby improving the extraction efficiency of dietary fiber.

[0018] Furthermore, the present invention determines whether the preparation of highland barley material meets the preset standard according to the explosion characteristic value, and when it does not meet the preset standard, increases the steam explosion pressure of the steam explosion, or performs a secondary judgment, thereby continuously optimizing and improving the preparation process, thereby improving the preparation efficiency of highland barley dietary fiber.

[0019] Furthermore, the present invention adds a high-temperature resistant α-amylase solution, an alkaline protease solution and a saccharifying enzyme to a highland barley suspension in sequence for enzymatic hydrolysis reaction and determines whether the preparation of the highland barley saccharification liquid meets a preset standard according to an enzymatic hydrolysis characteristic value, and monitors the enzymatic hydrolysis reaction process in real time, thereby improving the intelligence level of highland barley dietary fiber.

[0020] Furthermore, the present invention evaluates the quality of the preparation of the highland barley dietary fiber by determining whether the preparation of the highland barley dietary fiber meets a preset standard based on the characteristic absorbance value, thereby improving the stability of the preparation of the highland barley dietary fiber.

[0021] Furthermore, the present invention provides several pressure increasing methods for increasing the steam explosion pressure of the steam explosion, and each pressure increasing method increases the steam explosion pressure of the steam explosion by a different amount, thereby achieving precise control of the increase in the steam explosion pressure.

[0022] Furthermore, the present invention comprehensively considers the specific surface area and porosity of the highland barley material by setting a blasting characteristic value, thereby accurately evaluating the effect of the steam explosion treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The present invention is a flowchart of a method for preparing highland barley dietary fiber according to an embodiment of the present invention; Figure 2 The present invention is a flowchart of determining whether the preparation of highland barley material meets the preset standard according to the blasting characteristic value according to the embodiment of the present invention; Figure 3 The present invention is a flowchart of a method for secondarily determining whether the preparation of highland barley material meets a preset standard according to pore size distribution characterization parameters according to an embodiment of the present invention; Figure 4 The present invention is a flowchart for determining whether the preparation of highland barley saccharification liquid meets the preset standard. DETAILED DESCRIPTION

[0024] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0025] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0026] It should be pointed out that the data in this embodiment are obtained by comprehensive analysis and evaluation of the historical test data of the present invention three months before this test and the corresponding historical test results. It can be understood by those skilled in the art that the method of the present invention can determine the above parameters for a single item by selecting the value with the highest proportion as the preset standard parameter according to the data distribution, using weighted summation to use the obtained value as the preset standard parameter, substituting each historical data into a specific formula and using the value obtained by the formula as the preset standard parameter or other selection methods, as long as the method of the present invention can clearly define the different specific situations in the single determination process through the obtained values.

[0027] See also Figure 1 , Figure 2 , Figure 3 as well as Figure 4As shown, they are respectively a flow chart of a method for preparing highland barley dietary fiber according to an embodiment of the present invention; a flow chart of determining whether the preparation of highland barley material meets the preset standard according to the bursting characteristic value according to an embodiment of the present invention; a flow chart of determining whether the preparation of highland barley material meets the preset standard according to the pore size distribution characterization parameter according to an embodiment of the present invention; a flow chart of determining whether the preparation of highland barley saccharification liquid meets the preset standard according to an embodiment of the present invention.

[0028] On the one hand, an embodiment of the present invention provides a method for preparing highland barley dietary fiber, comprising: Step S1, sequentially steam-exploding, drying, grinding and screening highland barley particles to obtain highland barley material, obtaining the specific surface area and porosity of the highland barley material, obtaining the explosion characteristic value, and judging that the preparation of the highland barley material does not meet the preset standard according to the explosion characteristic value, judging whether the preparation of the highland barley material meets the preset standard for the second time according to the pore size distribution characterization parameter of the highland barley material, or increasing the steam explosion pressure of the steam explosion machine; in this embodiment, the highland barley is treated for 305 s under the condition of the steam explosion pressure of 0.6 MPa.

[0029] Step S2, after making a highland barley suspension from a highland barley material that meets the preset standard, the initial viscosity of the highland barley suspension is obtained by a viscometer, the initial reducing sugar content of the highland barley suspension is obtained by high performance liquid chromatography, and a high temperature resistant α-amylase solution is added to the highland barley suspension, and a magnetic stirrer is continuously stirred for 45 minutes under high temperature conditions to obtain a highland barley starch enzymolyzate; the high temperature value range in this step is [60°C, 70°C], and in this embodiment, enzymolysis reaction is carried out under the condition of being heated to 65°C; The optimum temperature range of thermostable α-amylase is usually between 60℃ and 70℃. In this temperature range, the enzyme has the highest activity and the fastest reaction rate. In this experiment, 65℃ was selected because this temperature is within the optimum temperature range, which can ensure that the enzyme can efficiently complete the hydrolysis of starch in a shorter time.

[0030] Step S3, after the highland barley starch hydrolysate is cooled to 45°C, the pH of the solution is adjusted, and then an alkaline protease solution is added. Under high temperature conditions, the magnetic stirrer is continuously stirred for 35 minutes to obtain a highland barley protein hydrolysate; in this step, the pH is adjusted to 9 using sodium carbonate, and the high temperature value range is [50°C, 70°C]. In this embodiment, the enzymolysis reaction is carried out under the condition of heating to 55°C; Step S4, after the highland barley protein enzymolysis liquid is cooled, the pH is adjusted to 5.5 with hydrochloric acid, and then a saccharifying enzyme is added, and the highland barley saccharification liquid is obtained by continuous stirring with a magnetic stirrer at a high temperature of 60° C. for 90 minutes, and the viscosity and reducing sugar content of the highland barley saccharification liquid are obtained, and the enzymolysis characteristic value is obtained, and it is determined whether the preparation of the highland barley saccharification liquid meets the preset standard according to the enzymolysis characteristic value; In this embodiment, the viscosity of the highland barley saccharification liquid is obtained by a viscometer, and the reducing sugar content of the highland barley saccharification liquid is obtained by high performance liquid chromatography; Step S5, adding ethanol solution to the highland barley saccharification liquid that meets the preset standard, naturally cooling to room temperature, standing for a preset time of 10 hours and then centrifuging, the obtained precipitate is highland barley dietary fiber, obtaining an absorption characteristic value of the highland barley dietary fiber, and determining whether the preparation of the highland barley dietary fiber meets the preset standard according to the absorption characteristic value; Specifically, the concentration of the ethanol solution is selected in the range of [70%, 95%], and the concentration of the ethanol solution in this embodiment is selected as 90%.

[0031] In this embodiment, 90% ethanol solution showed high efficiency in extracting highland barley dietary fiber. High concentration ethanol solution can better dissolve and extract effective ingredients in highland barley, such as β-glucan; 90% ethanol solution helps to form a more stable precipitate. After adding 90% ethanol solution to the highland barley saccharification solution, naturally cooling to room temperature and standing for 10 hours, a relatively pure highland barley dietary fiber precipitate can be obtained.

[0032] Step S6, drying the highland barley dietary fiber that meets the preset standard and storing it away from light.

[0033] Specifically, the process of determining, according to the blasting characteristic value, that the preparation of the highland barley material does not meet the preset standard includes: The blasting characteristic value is compared with the first preset blasting characteristic value 1.06 and the second preset blasting characteristic value 1.45 respectively; If the explosion characteristic value is less than the first preset explosion characteristic value, it is determined that the preparation of the highland barley material does not meet the preset standard, and the steam explosion pressure of the steam explosion is increased according to the difference between the first preset explosion characteristic value and the explosion characteristic value; If the blasting characteristic value is greater than or equal to the second preset blasting characteristic value, it is determined that the preparation of the highland barley material does not meet the preset standard, and a secondary determination is made based on the pore size distribution characterization parameter of the highland barley material whether the preparation of the highland barley material meets the preset standard.

[0034] Specifically, the process of obtaining the burst characteristic value includes: Weigh a highland barley sample of a preset weight of 5 g, load the highland barley sample into the sample cell of the specific surface area analyzer, introduce nitrogen, record the pressure change and adsorption amount during the highland barley sample adsorption of nitrogen, perform linear fitting according to the BET equation, and obtain the adsorption amount. The specific surface area is the ratio between the adsorption amount and the preset weight; The specific surface area is compared with the preset specific surface area of ​​0.38 cm 2 The ratio between 1 and 2 g is recorded as the explosion characteristic value.

[0035] Specifically, there are several pressure increasing methods for increasing the steam explosion pressure of the steam explosion, among which: If the explosion difference is less than the first preset explosion difference of 0.67, the first adjustment coefficient of 1.12 is used to increase the steam explosion pressure of the steam explosion; If the explosion difference is greater than or equal to the first preset explosion difference and less than the second preset explosion difference of 0.89, the second adjustment coefficient of 1.14 is used to increase the steam explosion pressure of the steam explosion; If the explosion difference is greater than or equal to the second preset explosion difference, the steam explosion pressure of the steam explosion is increased by using a third adjustment coefficient of 1.16; The blasting difference is the difference between the first preset blasting characteristic value and the blasting characteristic value.

[0036] Specifically, whether the preparation of the highland barley material meets the preset standard is determined again according to the pore size distribution characterization parameter, wherein: If the pore size distribution characterization parameter is less than a preset pore size distribution characterization threshold value of 0.71, it is determined that the preparation of the highland barley material meets the preset standard, and the highland barley material meeting the preset standard is made into a highland barley suspension; If the pore size distribution characterization parameter is greater than or equal to the preset pore size distribution characterization threshold, it is determined that the preparation of the highland barley material does not meet the preset standard, and the steam explosion time of the steam explosion machine is reduced according to the difference between the pore size distribution characterization parameter and the preset pore size distribution characterization threshold; The pore size distribution characterization parameter is the pore size standard deviation of the highland barley material, and the pore size of the highland barley material is obtained by scanning electron microscopy.

[0037] Specifically, whether the preparation of the highland barley saccharification liquid meets the preset standard is determined according to the enzymolysis characteristic value, wherein: If the enzymolysis characteristic value is less than the preset enzymolysis characteristic threshold value of 0.77, it is determined that the preparation of the highland barley saccharification liquid does not meet the preset standard, and the stirring rate of the magnetic stirrer is synchronously increased according to the difference between the preset enzymolysis characteristic threshold value and the enzymolysis characteristic value; If the enzymolysis characteristic value is greater than or equal to the preset enzymolysis characteristic threshold, it is determined that the preparation of the highland barley saccharification liquid meets the preset standard, and the highland barley saccharification liquid that meets the preset standard is centrifuged.

[0038] Specifically, the enzymatic hydrolysis characteristic value is calculated by the following formula: ; Wherein, P represents the enzymatic hydrolysis characteristic value; η0 represents the initial viscosity of highland barley suspension; η1 represents the viscosity of highland barley saccharification liquid; λ1 represents the viscosity weight, λ1=0.65; DE0 represents the initial reducing sugar content of highland barley suspension; DE1 represents the reducing sugar content of highland barley saccharification liquid; λ2 represents the weight of the reducing sugar content of highland barley saccharification liquid, λ2=0.35.

[0039] Specifically, whether the preparation of the highland barley dietary fiber meets the preset standard is determined according to the absorbance characteristic value, wherein: If the absorbance characteristic value is less than a preset absorbance threshold value of 1.22, it is determined that the preparation of the highland barley dietary fiber does not meet the preset standard, and the speed of the centrifuge is increased according to the difference between the preset absorbance threshold value and the absorbance characteristic value; If the absorbance characteristic value is greater than or equal to the preset absorbance threshold value, it is determined that the preparation of the highland barley dietary fiber meets the preset standard; The absorbance characteristic value is the ratio between the absorbance of the highland barley dietary fiber and a preset absorbance of 0.75, and the absorbance is obtained by a spectrophotometer.

[0040] Specifically, the increase in the rotation speed of the centrifuge is positively correlated with the absorbance difference, wherein the positive correlation is, for example, a linear positive correlation or a nonlinear positive correlation, and the linear slope of the linear positive correlation is not specifically limited. It can be understood that the greater the absorbance difference, the greater the increase in the rotation speed of the centrifuge; the absorbance difference is the difference between the preset absorbance threshold and the absorbance characteristic value.

[0041] On the other hand, the present invention also provides highland barley dietary fiber prepared according to the preparation method of highland barley dietary fiber, wherein the ratio of soluble dietary fiber to insoluble dietary fiber of the highland barley dietary fiber is 13:9.

[0042] Example 1: Weigh 1 g of a highland barley dietary fiber sample prepared only by enzymatic hydrolysis, and weigh 1 g of a highland barley dietary fiber sample prepared by combining steam explosion and enzymatic hydrolysis of the present invention, and put them into identical weighing bottles respectively; Add an excess of distilled water to the weighing bottle so that the sample is completely submerged in the water, cover the bottle cap, and let it stand for 2 hours; Take out the samples from the weighing bottles and gently absorb the excess water on the surface with filter paper; The samples after surface moisture was removed were weighed respectively, and the weight of the highland barley dietary fiber sample prepared only by the enzymatic hydrolysis method after absorbing water was recorded to be 2.1 g, and the weight of the highland barley dietary fiber sample prepared by the present invention after absorbing water was 4.5 g; Therefore, it can be concluded that the highland barley dietary fiber sample prepared by the present invention has a stronger water absorption capacity. After steam explosion and enzymatic hydrolysis, the structure of the highland barley dietary fiber becomes more loose and porous, and the specific surface area increases, so that the fiber can better absorb and retain water, improve the water holding capacity, and thus improve the quality of the highland barley dietary fiber.

[0043] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

[0044] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing highland barley dietary fiber, characterized in that: include: Sequentially steam-exploding, drying, grinding and screening highland barley particles to obtain highland barley material, obtaining the specific surface area and porosity of the highland barley material, and obtaining a blasting characteristic value. When it is determined that the preparation of the highland barley material does not meet the preset standard according to the blasting characteristic value, it is secondarily determined whether the preparation of the highland barley material meets the preset standard according to the pore size distribution characterization parameter of the highland barley material, or the steam explosion pressure of the steam blasting machine is increased; After making highland barley material that meets the preset standard into highland barley suspension, obtaining the initial viscosity and initial reducing sugar content of the highland barley suspension, adding a high temperature resistant α-amylase solution into the highland barley suspension, and continuously stirring with a magnetic stirrer under high temperature conditions to obtain a highland barley starch enzymolysis solution; After the highland barley starch enzymolyzate is cooled, the pH of the solution is adjusted and then an alkaline protease solution is added, and the highland barley protein enzymolyzate is obtained by continuous stirring with a magnetic stirrer under high temperature conditions; After the highland barley protein enzymolysis liquid is cooled, the pH value of the solution is adjusted and then a saccharifying enzyme is added, and a magnetic stirrer is continuously stirred under high temperature conditions to obtain a highland barley saccharification liquid, and the viscosity and reducing sugar content of the highland barley saccharification liquid are obtained to obtain an enzymolysis characteristic value, and whether the preparation of the highland barley saccharification liquid meets a preset standard is determined according to the enzymolysis characteristic value; Adding an ethanol solution to a highland barley saccharification liquid that meets a preset standard, naturally cooling to room temperature, standing for a preset time, and then centrifuging, the resulting precipitate is highland barley dietary fiber, obtaining an absorption characteristic value of the highland barley dietary fiber, and determining whether the preparation of the highland barley dietary fiber meets the preset standard based on the absorption characteristic value; The highland barley dietary fiber that meets the preset standards is dried and stored away from light.

2. The method for preparing highland barley dietary fiber according to claim 1, characterized in that: The process of determining, according to the blasting characteristic value, that the preparation of the highland barley material does not meet the preset standard comprises: Comparing the blasting characteristic value with the first preset blasting characteristic value and the second preset blasting characteristic value respectively; If the explosion characteristic value is less than the first preset explosion characteristic value, it is determined that the preparation of the highland barley material does not meet the preset standard, and the steam explosion pressure of the steam explosion is increased according to the difference between the first preset explosion characteristic value and the explosion characteristic value; If the blasting characteristic value is greater than or equal to the second preset blasting characteristic value, it is determined that the preparation of the highland barley material does not meet the preset standard, and a secondary determination is made based on the pore size distribution characterization parameter of the highland barley material whether the preparation of the highland barley material meets the preset standard.

3. The method for preparing highland barley dietary fiber according to claim 2, characterized in that, The process of obtaining the burst characteristic value includes: Weigh a highland barley sample of a preset weight, load the highland barley sample into a sample cell of a specific surface area analyzer, introduce nitrogen, record the pressure change and adsorption amount during the highland barley sample adsorption of nitrogen, perform linear fitting according to the BET equation, calculate the adsorption amount, and the specific surface area is the ratio between the adsorption amount and the preset weight; The ratio between the specific surface area and the preset specific surface area is recorded as the explosion characteristic value.

4. The method for preparing highland barley dietary fiber according to claim 3, characterized in that: There are several pressure increasing methods for increasing the steam explosion pressure of the steam explosion, and each pressure increasing method has a different increase range for the steam explosion pressure of the steam explosion.

5. The method for preparing highland barley dietary fiber according to claim 4, characterized in that: According to the pore size distribution characterization parameter, it is secondarily determined whether the preparation of the highland barley material meets the preset standard, wherein: If the pore size distribution characterization parameter is less than a preset pore size distribution characterization threshold, it is determined that the preparation of the highland barley material meets the preset standard, and the highland barley material meeting the preset standard is made into a highland barley suspension; If the pore size distribution characterization parameter is greater than or equal to the preset pore size distribution characterization threshold, it is determined that the preparation of the highland barley material does not meet the preset standard, and the steam explosion time of the steam explosion machine is reduced according to the difference between the pore size distribution characterization parameter and the preset pore size distribution characterization threshold; The pore size distribution characterization parameter is the pore size standard deviation of highland barley material.

6. The method for preparing highland barley dietary fiber according to claim 5, characterized in that: According to the enzymolysis characteristic value, it is determined whether the preparation of the highland barley saccharification liquid meets the preset standard, wherein: If the enzymolysis characteristic value is less than the preset enzymolysis characteristic threshold, it is determined that the preparation of the highland barley saccharification liquid does not meet the preset standard, and the stirring rate of the magnetic stirrer is synchronously increased according to the difference between the preset enzymolysis characteristic threshold and the enzymolysis characteristic value; If the enzymolysis characteristic value is greater than or equal to the preset enzymolysis characteristic threshold, it is determined that the preparation of the highland barley saccharification liquid meets the preset standard, and the highland barley saccharification liquid that meets the preset standard is centrifuged.

7. The method for preparing highland barley dietary fiber according to claim 6, characterized in that: The enzymatic hydrolysis characteristic value is determined by the viscosity change and reducing sugar content change of the highland barley suspension.

8. The method for preparing highland barley dietary fiber according to claim 7, characterized in that: According to the absorbance characteristic value, it is determined whether the preparation of the highland barley dietary fiber meets the preset standard, wherein: If the absorbance characteristic value is less than a preset absorbance threshold value, it is determined that the preparation of the highland barley dietary fiber does not meet the preset standard, and the speed of the centrifuge is increased according to the difference between the preset absorbance threshold value and the absorbance characteristic value; If the absorbance characteristic value is greater than or equal to the preset absorbance threshold value, it is determined that the preparation of the highland barley dietary fiber meets the preset standard; The absorbance characteristic value is the ratio between the absorbance of the highland barley dietary fiber and a preset absorbance.

9. The method for preparing highland barley dietary fiber according to claim 8, characterized in that: The increase range of the rotation speed of the centrifuge is positively correlated with the absorbance difference, wherein the absorbance difference is the difference between the preset absorbance threshold and the absorbance characteristic value.

10. A highland barley dietary fiber prepared according to the method for preparing highland barley dietary fiber according to any one of claims 1 to 9, characterized in that: The ratio of soluble dietary fiber to insoluble dietary fiber in the highland barley dietary fiber is 13:9.

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