Anti-wrinkle composition, application and skin care product

Abstract

The invention belongs to the technical field of skin care, and discloses an anti-wrinkle composition, application and a skincare product, the anti-wrinkle composition comprises the following components by weight: 1-3 parts of grape seed oil, 0.4-1 part of a purple coneflower extract, and 0.2-0.6 part of a tulip flower extract. The grape seed oil, the purple coneflower extract and the tulip flower extract are compounded, so that the effect of synergistically improving the anti-wrinkle effect of the skin is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of skin care, and in particular to an anti-wrinkle composition, use and skin care product. Background Art

[0002] As we age, the collagen and elastic fibers in the skin gradually decrease, causing the skin to lose elasticity and form wrinkles, which can make a person's appearance look more aged. Therefore, many people now pay more attention to skin anti-wrinkle, hoping to delay aging through anti-wrinkle products. Anti-wrinkle care can effectively protect the skin, slow down the symptoms of aging, and make the whole person look younger; therefore, the research and development of anti-wrinkle products is a continuous research direction in skin care.

[0003] Prior art 1: Chinese patent application 201711180790.6 discloses a moisturizing and anti-wrinkle daily film composition and its preparation method. The application description states: "Three collagens, namely hydrolyzed wheat protein, hydrolyzed collagen and hydrolyzed soy protein, are compounded to form a moisturizing protective film on the skin surface, enhance the adhesion of the extracellular matrix of the epidermal cells, maintain the firmness of the epidermal lipid monolayer, increase the cellular protein level, promote the synthesis of collagen, and thus synergistically enhance the anti-wrinkle effect."

[0004] Prior art 2: Chinese patent application 201810629118.9 discloses a skin care composition with moisturizing and anti-wrinkle effects and its application. The skin care composition comprises the following raw material components in parts by weight: 5×10 human stem cell factor -7 ~1×10 -5 parts, 1 to 5 parts of collagen, 0.03 to 2 parts of chitosan, and 0.2 to 1 part of sodium hyaluronate; the specification of prior art 2 states: "Collagen is a polypeptide mixture, which is similar to the collagen structure of human skin and has good compatibility. It can penetrate into the deep layer of human skin after percutaneous transdermal treatment, provide the skin with necessary nutrients, maintain the stability and integrity of the collagen fiber structure, enhance the collagen activity in the skin, improve the living environment of skin cells, promote tissue metabolism, and achieve the purpose of nutrition, moisturizing and beauty."

[0005] Both prior art 1 and prior art 2 increase the collagen activity in the skin and promote the synthesis of collagen by directly providing collagen. The methods are direct and effective and are technical solutions worthy of reference. However, although the overly direct method can achieve relatively excellent anti-wrinkle effects in a short period of time, excessive use may also cause allergic reactions. Excessive use of skin care products containing collagen may affect skin microcirculation and bring burden to the skin.

[0006] Therefore, it is necessary to develop an anti-wrinkle composition with lower allergic reaction to provide effective anti-wrinkle effect for the skin. Summary of the invention

[0007] One of the purposes of the present invention is to provide an anti-wrinkle composition, which is compounded with grape seed oil, purple coneflower extract and tulip flower extract as plant extracts, and performs skin anti-wrinkle care by compounding plant extracts, which has the advantages of being mild and having excellent anti-wrinkle effects.

[0008] Another object of the present invention is to provide an anti-wrinkle composition for use in preparing cosmetics, which can effectively provide excellent skin anti-wrinkle and moisturizing effects.

[0009] At the same time, the present invention also provides a skin care product containing the anti-wrinkle composition of the present invention, which has the advantages of being mild and having excellent anti-wrinkle effect.

[0010] To achieve the above object, the present invention provides an anti-wrinkle composition, comprising the following components by weight: 1-3 parts of grape seed oil, 0.4-1 parts of purple coneflower extract, and 0.2-0.6 parts of tulip flower extract.

[0011] Grape seed oil is rich in antioxidant ingredients such as vitamin E and proanthocyanidins, which can remove free radicals in the body and reduce the damage of free radicals to the skin, thereby delaying aging and reducing the formation of wrinkles. Proanthocyanidins have the effect of promoting collagen synthesis. Collagen is a key component for skin elasticity and firmness. Increasing collagen synthesis can improve skin sagging and reduce wrinkles.

[0012] Echinacea extract contains a variety of antioxidant ingredients, such as chicoric acid and caffeic acid. These ingredients have strong antioxidant and free radical scavenging capabilities, which can reduce skin oxidative stress and thus delay skin aging; Echinacea extract can stimulate epithelial cell proliferation and collagen synthesis, accelerate the wound healing process, thereby improving skin elasticity and firmness, and reducing the formation of wrinkles; Echinacea extract can dilate blood vessels, increase local blood flow, improve skin microcirculation, promote the delivery of nutrients and the excretion of waste, and maintain the health of the skin.

[0013] Tulip flower extract is rich in various antioxidants, such as flavonoids, which can effectively neutralize free radicals and reduce the damage of free radicals to skin cells. Tulip flower extract also contains cyanidin, salicylic acid, arginine and other ingredients, which also play an important role in anti-aging. Cyanocyanidin has anti-inflammatory and antioxidant effects, which can reduce inflammatory responses and oxidative damage to the skin. Salicylic acid helps remove aging cells in the stratum corneum and promotes skin metabolism.

[0014] The present invention effectively improves the antioxidant effect of the composition by compounding grape seed oil, purple coneflower extract and tulip flower extract, while also promoting collagen synthesis, skin metabolism and improving skin microcirculation, thereby synergistically improving the skin's anti-wrinkle effect.

[0015] Furthermore, the anti-wrinkle composition of the present invention also includes a solvent and an emulsifier;

[0016] Furthermore, the solvent is water, and the emulsifier is PEG-40 hydrogenated castor oil.

[0017] Preferably, in order to further improve the anti-wrinkle and moisturizing effects of the composition, the anti-wrinkle composition of the present invention further comprises 2-4 parts of maltodextrin.

[0018] Maltodextrin contains a certain amount of vitamin E, which can provide a certain improvement in the anti-wrinkle and moisturizing effects of the composition.

[0019] Preferably, in order to further improve the anti-wrinkle and moisturizing effects of the composition, the anti-wrinkle composition of the present invention further comprises 0.1-0.3 parts of Phellodendron amurense bark extract.

[0020] The present invention simultaneously provides a use of the anti-wrinkle composition in preparing cosmetics.

[0021] Meanwhile, the present invention also provides a skin care product containing 0.1-20wt% of the anti-wrinkle composition.

[0022] Beneficial Effects

[0023] Compared with the prior art, the present invention has at least the following advantages:

[0024] (1) Grape seed oil is rich in antioxidant ingredients such as vitamin E and proanthocyanidins, which can remove free radicals in the body and reduce the damage of free radicals to the skin, thereby delaying aging and reducing the formation of wrinkles; proanthocyanidins have the effect of promoting collagen synthesis. Collagen is a key component of skin elasticity and firmness. Increasing collagen synthesis can improve skin sagging and reduce wrinkles;

[0025] (2) Echinacea extract contains a variety of antioxidant ingredients, such as chicoric acid and caffeic acid. These ingredients have strong antioxidant and free radical scavenging capabilities, which can reduce skin oxidative stress and thus delay skin aging; Echinacea extract can stimulate epithelial cell proliferation and collagen synthesis, accelerate the wound healing process, thereby improving skin elasticity and firmness and reducing the formation of wrinkles; Echinacea extract can dilate blood vessels, increase local blood flow, improve skin microcirculation, promote the delivery of nutrients and the discharge of waste, and maintain the health of the skin;

[0026] (3) Tulip flower extract is rich in a variety of antioxidant substances, such as flavonoids, which can effectively neutralize free radicals and reduce the damage of free radicals to skin cells. Tulip flower extract also contains ingredients such as cyanidin, salicylic acid, and arginine, which also play an important role in anti-aging. Cyanocyanidin has anti-inflammatory and antioxidant effects, which can reduce the inflammatory response and oxidative damage of the skin; salicylic acid helps to remove aging cells in the stratum corneum and promote skin metabolism.

[0027] (4) The present invention produces a synergistic effect of enhancing the anti-wrinkle effect of the skin by compounding grape seed oil, purple coneflower extract and tulip flower extract. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with the embodiments, but it does not constitute any limitation to the present invention. Any limited number of modifications made within the scope of the claims of the present invention are still within the scope of the claims of the present invention.

[0029] In order to explain the technical content of the present invention in detail, further description is given below in conjunction with the implementation modes.

[0030] In the following examples and comparative examples, the grape seed oil was purchased from Northstar Lipids (UK) Ltd.; purple echinacea extract and sea fennel extract were purchased from Gansu Yishengxiang Biotechnology Co., Ltd., and the properties were powder; tulip flower extract and phellodendron bark extract were purchased from Ningxia Vanilla Biotechnology Co., Ltd., and the properties were powder; maltodextrin was purchased from Xi'an Jinxiang Pharmaceutical Excipients Co., Ltd., and the properties were powder; jojoba seed oil was purchased from Jiangxi Xinsen Natural Plant Oil Co., Ltd.; white willow bark extract was purchased from Xi'an Guohao Biotechnology Co., Ltd., and the properties were powder; pine bark extract was purchased from Xi'an Rongzhen Biotechnology Co., Ltd., and the properties were powder; PEG-40 hydrogenated castor oil was purchased from Wuhan Lanabai Pharmaceutical Chemical Co., Ltd.

[0031] The technical solution of the present invention can be implemented by other commercially available products, and is not limited to the raw materials from the above sources.

[0032] Unless otherwise specified, in the following examples and comparative examples, the parts and % refer to parts by weight and percentage by weight, respectively.

[0033] According to the formula table in Table 1, the composition is prepared as follows:

[0034] Step 1: Mix grape seed oil and PEG-40 hydrogenated castor oil evenly, add them into deionized water, and heat to 80°C until the materials are completely dissolved;

[0035] Step 2: Cool down to 40°C, add Echinacea purpurea extract and tulip flower extract and stir until completely dissolved.

[0036] It should be noted that in the following examples and comparative examples, PEG-40 hydrogenated castor oil and deionized water are respectively an emulsifier and a solvent, the main purpose of which is to mix grape seed oil, Echinacea purpurea extract and tulip flower extract, which has no effect on the efficacy of the composition.

[0037]

[0038]

[0039] Efficacy Test

[0040] 1. Test method: Elastase inhibition rate

[0041] 1. Purpose and principle of the experiment

[0042] The anti-wrinkle effect is mainly characterized by evaluating the inhibition rate of test samples on elastase. Elastase is mainly synthesized and secreted by fibroblasts, which can degrade elastin in the skin and cause skin aging. The experimental principle of inhibiting elastase is that porcine pancreatic elastase catalyzes the reaction with the enzyme substrate. After adding the active substance, the absorbance changes. The size of the absorbance change reflects the inhibition rate of the elastase inhibitor.

[0043] This test refers to the laboratory method (HMC-WI-028 elastase inhibition rate) and compares the elastase inhibition rate test results of the test sample with that of the negative control. If the inhibition rate of the test sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain anti-wrinkle effect.

[0044] This test method is an in vitro method suitable for evaluating cosmetic products that claim to achieve anti-wrinkle efficacy by inhibiting elastase.

[0045] 2 Test indicators

[0046] Elastase inhibition rate determination standard: If the elastase inhibition rate of the sample is higher than that of the negative control and there is a significant difference (P<0.05), it can be considered that the test sample has a certain anti-wrinkle effect.

[0047] 3 Experimental materials and methods

[0048] 3.1 Instruments and Equipment

[0049] BSA224S Analytical Balance

[0050] RT-6100 ELISA Analyzer

[0051] 3.2 Reagents

[0052] Elastase (Porcine Pancreas), BR

[0053] N-Succinyl-L-Alanyl-L-Alanyl-L-Alanine, 98%

[0054] Epigallocatechin gallate (EGCG), 98%

[0055] 3.3 Test methods

[0056] (1) Treatment of control and test samples

[0057] Sample group: 100% of Examples 1-7 and Comparative Examples 1-6;

[0058] Positive control (EGCG): diluted with water to a positive control concentration of 0.1%;

[0059] Negative control: pure water.

[0060] (2) Test operation steps

[0061] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25℃ for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0062] (3) Calculation formula

[0063]

[0064] Where: A—is the absorbance of the reaction solution without sample;

[0065] B—is the absorbance of the reaction solution without sample and enzyme;

[0066] C—is the absorbance of the reaction solution containing sample and enzyme;

[0067] D—is the absorbance of the reaction solution containing sample and without enzyme.

[0068] (4) Data analysis

[0069] The statistical analysis software was SPSS, and the comparison of the elastase inhibition rate between the test sample, positive control and negative control was performed by independent sample t test. The above statistical analysis was two-tailed test, and the significance level was α = 0.05. P>0.05 indicated that there was no significant difference between the two groups; P<0.05 indicated that there was a significant difference between the two groups.

[0070] The anti-wrinkle compositions of Examples 1-7 and Comparative Examples 1-9 were tested according to the above method, and the results are shown in Table 2;

[0071] Table 2 Anti-wrinkle efficacy test results of the anti-wrinkle compositions of Examples 1-7 and Comparative Examples 1-9

[0072] Elastase inhibition rate % P-value Example 1 45.93 ＜0.050 Example 2 44.17 ＜0.050 Example 3 44.55 ＜0.050 Example 4 43.86 ＜0.050 Example 5 49.24 ＜0.050 Example 6 57.82 ＜0.050 Example 7 48.99 ＜0.050 Comparative Example 1 25.74 ＜0.050 Comparative Example 2 27.13 ＜0.050 Comparative Example 3 27.46 ＜0.050 Comparative Example 4 29.40 ＜0.050 Comparative Example 5 22.47 ＜0.050 Comparative Example 6 26.38 ＜0.050 Comparative Example 7 28.63 ＜0.050 Comparative Example 8 29.97 ＜0.050 Comparative Example 9 30.14 ＜0.050 Negative control -0.87 / Positive Control 80.09 ＜0.050

[0073] According to the results in Table 2:

[0074] According to Examples 1-4, it can be seen that the compounding of grape seed oil, purple echinacea extract and tulip flower extract in the present invention has obvious elastase inhibition effect; at the same time, according to the data of Examples 1 and 5, it can be seen that adding maltodextrin with anti-wrinkle effect on the basis of the compounding of grape seed oil, purple echinacea extract and tulip flower extract can further improve the anti-wrinkle effect of the composition, but compared with Example 6, the improvement of the anti-wrinkle effect of maltodextrin is not obvious.

[0075] According to the data comparison between Example 1 and Comparative Examples 1-3, in the absence of any one of grape seed oil, purple echinacea extract and tulip flower extract, the elastase inhibition rate exerted by the compositions of Comparative Examples 1-3 is significantly lower than that of Example 1, indicating that the present invention produces a synergistic effect of improving the skin anti-wrinkle effect of the composition through the compounding of grape seed oil, purple echinacea extract and tulip flower extract.

[0076] According to the data comparison between Example 1 and Comparative Examples 4-6, when Comparative Example 4 uses jojoba seed oil to replace grape seed oil, Comparative Example 5 uses sea fennel extract to replace purple coneflower extract, and Comparative Example 6 uses tulip flower extract to replace white willow bark extract, although it exerts a certain skin anti-wrinkle effect and achieves a good elastase inhibition rate; however, compared with the technical solution of the present invention, the elastase inhibition rate thereof is significantly reduced, indicating that the present invention produces a synergistic effect of improving the skin anti-wrinkle effect of the composition through the compounding of grape seed oil, purple coneflower extract and tulip flower extract.

[0077] According to the data comparison of Example 1, Example 6 and Example 7, it can be seen that on the basis of the compound of grape seed oil, purple echinacea extract and tulip flower extract, the elastase inhibition rate of the composition is greatly improved by adding the Phellodendron bark extract in Example 6, while when the pine bark extract with similar efficacy to the Phellodendron bark extract is added in Example 7, only a small increase in the elastase inhibition rate is obtained. It is speculated that on the basis of the compound of grape seed oil, purple echinacea extract and tulip flower extract, the Phellodendron bark extract, grape seed oil, purple echinacea extract and tulip flower extract have a further synergistic effect of enhancing the skin anti-wrinkle effect of the composition.

[0078] In order to further verify this speculation, the applicant verified the synergistic source of the Phellodendron bark extract through Comparative Examples 7-9, and found that the substantial improvement in the elastase inhibition rate of Example 6 is based on the compounding of grape seed oil, purple echinacea extract, and tulip flower extract. In the case where Comparative Examples 7-9 lacked grape seed oil, purple echinacea extract, and tulip flower extract, respectively, the elastase inhibition rates shown were different, but compared with the elastase inhibition effect of Example 6, there was a significant decrease, which verified that the synergistic effect of Phellodendron bark extract with grape seed oil, purple echinacea extract, and tulip flower extract needs to be based on the compounding of grape seed oil, purple echinacea extract, and tulip flower extract. The compounding of Phellodendron bark extract, grape seed oil, purple echinacea extract, and tulip flower extract further synergistically enhances the skin anti-wrinkle effect of the composition.

[0079] 2. Moisturizing effect

[0080] Test method: The test site should not use any products (cosmetics or topical drugs) 2 to 3 days before the test, and should not come into contact with water for 1h to 3h. After entering the laboratory, the subjects wiped the inner side of both forearms with dry paper towels, marked 17 test sites, and randomly divided them into 16 sample areas and 1 control area. The sample area was smeared with the test substance, and the control area was used as a control. The test area was 3cm×3cm, exposed for 20min, and the water content of the skin stratum corneum before the test site sample was applied (T0) was tested, and then the water content was calculated at (2.0±0.1)mg / cm 2 The test substance was applied in the sample area at a dose of 100 mg / kg. After application, the subjects returned home on their own and the test area was not allowed to come into contact with water. The water content of the stratum corneum of the test area was tested again 1 hour (T1) after application.

[0081] Before the formal test, the subjects must sit quietly in a standard room for at least 20 minutes without drinking water or beverages. Each part is tested at 3 points each time, and the average value is taken. During the test, the subject's forearm is exposed and placed in the test position, and remains relaxed.

[0082] Instruments and equipment and evaluation instructions

[0083] Table 2 Test instrument information

[0084] Instrument Name Model Specifications Skin Moisture Tester CorneometerCM825 Electronic balance Practum224-1CN

[0085] Water content of skin stratum corneum: Use a skin moisture tester to test the selected area 3 times and record the average value.

[0086] Parameter description: The moisture content of the skin stratum corneum; the larger the measured value, the higher the moisture content of the skin stratum corneum.

[0087] Result determination: After several hours of trial use of the product, the water content of the stratum corneum of the skin in the sample area increased significantly or the water content of the stratum corneum of the skin in the sample area increased significantly compared with the control area, indicating that the test product has skin moisturizing effect.

[0088] Test Results

[0089] SPSS software was used to perform descriptive statistics on each test value. The Shapiro-Wilk Test was used to test the significance of the normal distribution of the data change value. If P>0.05, it was normally distributed, and the paired sample t test was performed, with a significant difference level of α=0.05. If P<0.05, it was non-normally distributed, and the Wilcoxon signed rank test was performed.

[0090] The moisturizing compositions obtained in Examples 1-7 and Comparative Examples 1-9 were tested according to the above method. The results are shown in Table 3, wherein T1(1h) change rate=(T1(1h)-T0(0h)) / T0(0h)*100%;

[0091] Table 1 Moisturizing efficacy test results of the moisturizing compositions obtained in Examples 1-7 and Comparative Examples 1-9

[0092]

[0093]

[0094]

[0095] According to Table 3, we can see that:

[0096] According to Examples 1-4, it can be seen that the present invention has obvious moisturizing effect through the compounding of grape seed oil, purple echinacea extract and tulip flower extract; but unlike the anti-wrinkle effect, Example 5 of the present invention adds maltodextrin on the basis of the compounding of grape seed oil, purple echinacea extract and tulip flower extract, which can greatly improve the moisturizing effect of the composition. It is speculated that this may be because maltodextrin and grape seed oil, purple echinacea extract and tulip flower extract produce a synergistic effect of improving the moisturizing effect of the composition; while Examples 6 and 7, on the basis of the compounding of grape seed oil, purple echinacea extract and tulip flower extract, add Phellodendron bark extract and Pine bark extract respectively, but do not show a significant improvement in moisturizing effect, indicating that the addition of Phellodendron bark extract and Pine bark extract only produces a simple moisturizing effect superposition effect.

[0097] Similarly, according to the data comparison between Example 1 and Comparative Examples 1-3, in the absence of any one of grape seed oil, purple echinacea extract and tulip flower extract, the moisturizing effect of the compositions of Comparative Examples 1-3 is significantly reduced compared with that of Example 1, indicating that the present invention synergistically improves the skin moisturizing effect of the composition through the compounding of grape seed oil, purple echinacea extract and tulip flower extract.

[0098] According to the data comparison of Example 1 and Comparative Examples 4-6, when Comparative Example 4 uses jojoba seed oil to replace grape seed oil, Comparative Example 5 uses sea fennel extract to replace purple coneflower extract, and Comparative Example 6 uses tulip flower extract to replace white willow bark extract, although it exerts a certain skin moisturizing effect and achieves a good skin moisturizing effect; however, compared with the technical solution of the present invention, the moisturizing effect thereof is significantly reduced, indicating that the present invention produces a synergistic effect of improving the skin moisturizing effect of the composition through the compounding of grape seed oil, purple coneflower extract and tulip flower extract.

[0099] Since the addition of the Phellodendron bark extract only produces a simple superposition of moisturizing effects, the moisturizing effects of Comparative Examples 7-9 are also correspondingly improved to a certain extent compared with Comparative Examples 1-3. However, compared with Example 1 of the present invention, the improvement in effect is not obvious, indicating that the present invention produces a synergistic effect of improving the skin moisturizing effect of the composition through the compounding of grape seed oil, purple coneflower extract and tulip flower extract.

[0100] The embodiments presented herein are only embodiments selected according to the combination of all possible embodiments. The appended claims should not be limited by the embodiments describing the present invention. Some numerical ranges used in the claims include sub-ranges therein, and the changes in these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle composition, characterized in that: The composition comprises the following components by weight: 1-3 parts of grape seed oil, 0.4-1 parts of purple coneflower extract and 0.2-0.6 parts of tulip flower extract.

2. The anti-wrinkle composition according to claim 1, characterized in that Also includes solvents and emulsifiers.

3. The anti-wrinkle composition according to claim 1, characterized in that Also includes 2-4 portions of maltodextrin.

4. The anti-wrinkle composition according to claim 1, characterized in that It also includes 0.1-0.3 parts of Phellodendron amurense bark extract.

5. Use of the anti-wrinkle composition according to any one of claims 1 to 4 in preparing cosmetics.

6. A skin care product, characterized in that: Contains 0.1-20wt% of the anti-wrinkle composition according to any one of claims 1-4.

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