Oligosaccharide syrup for enhancing movement performance of body and application of oligosaccharide syrup

Abstract

The invention discloses oligosaccharide syrup capable of enhancing body movement performance and application of the oligosaccharide syrup, and belongs to the technical field of functional food. The oligosaccharide syrup provided by the invention is refined crystalline glucose mother liquor chromatography raffinate (BD liquid) or an enzyme treatment product thereof, and the main components of the oligosaccharide syrup are glucose, maltooligosacharide and isomaltooligosacharide. The oligosaccharide syrup can significantly increase exhaustive swimming time of mice and improve blood sugar level, and in detection of biochemical indexes related to energy metabolism, the oligosaccharide syrup can reduce levels of movement metabolites such as serum lactic acid, serum urea nitrogen and the like of the mice, increase contents of hepatic glycogen and muscle glycogen, and reduce activity of lactic dehydrogenase and creatine kinase in serum; the oligosaccharide syrup can also relieve oxidative stress of mice after exercise, reduce the content of lipid peroxide malonaldehyde and improve the activity of two antioxidant enzymes, namely superoxide dismutase and glutathione peroxidase. Therefore, the oligosaccharide syrup provided by the invention has the effects of relieving exercise-induced fatigue and improving endurance, and has a very considerable application prospect in the field of sports nutritious foods.

Description

Technical Field

[0001] The invention belongs to the technical field of functional foods, and in particular relates to an oligosaccharide syrup for enhancing body athletic performance and an application thereof. Background Art

[0002] Generally speaking, fatigue can be divided into central fatigue and peripheral fatigue. The latter is also called sports fatigue, which is an adverse physiological state caused by strenuous exercise, usually manifested as general fatigue, muscle soreness, and limited movement. The main causes of sports fatigue are the accumulation of exercise metabolites, the exhaustion of energy substances, and the body's oxidative stress. After high-intensity training, excessive lactic acid will accumulate in skeletal muscle, causing a decrease in muscle pH and a decrease in energy supply, thereby reducing endurance and causing fatigue. Liver glycogen and muscle glycogen are the main sources of energy for the body in a fatigued state. When the glycogen content is at a low level, the body will absorb energy through protein. However, urea nitrogen produced by protein metabolism will also damage muscle cells and reduce endurance. In addition, strenuous exercise will also break the balance between oxidation and anti-oxidation in the body, leading to excessive production of reactive oxygen and malondialdehyde, and ultimately causing physical fatigue of the body. Although some drugs have been developed to overcome sports fatigue, the drugs themselves also have disadvantages such as large side effects and short onset time. Therefore, it is more promising to use low-toxic anti-fatigue supplements from natural sources to replace anti-fatigue drugs.

[0003] Sports nutrition foods are products that serve as dietary supplements to meet the metabolic needs of athletes. During exercise, the body will enter a state of nutrient loss. In order to prevent damage to the body, relevant nutrients need to be replenished in a timely manner. Carbohydrates are important energy substances in the human body. Increasing the intake of exogenous carbon water during the exercise stage can maintain the body's high carbon water oxidation rate and normal blood sugar levels, thereby improving endurance and delaying exhaustion. Glucose is often used as a source of carbon water in traditional sports nutrition foods. Although glucose can be quickly absorbed by the body, it cannot maintain blood sugar levels. Currently, some glucose polymers such as maltooligosaccharides and maltodextrin are added to new sports nutrition foods to prolong energy supply and enhance endurance.

[0004] At present, the industrial production of crystalline glucose mainly uses starch as raw material, which is converted into glucose syrup by double enzyme method, and then obtains glucose crystals after filtering, decolorizing, ion exchange, concentration, crystallization, centrifugation, drying and other processes. After the first crystallization process, glucose syrup will produce glucose primary mother liquor, the DE value of which is about 85. After a little refining and concentration, it can be crystallized again to obtain crystalline glucose and glucose secondary mother liquor. The purity of glucose in the secondary mother liquor is low, and it is difficult to crystallize again. In addition to glucose, it also contains a large amount of oligosaccharides and impurities, with complex ingredients and greater difficulty in use. Some starch sugar companies will use chromatographic separation technology to extract glucose from the secondary mother liquor, which further increases the glucose yield, but will eventually produce a large amount of chromatographic residual liquid (BD liquid). The glucose content in BD liquid is relatively low, about 25% to 30%, and the remaining components are oligosaccharides mainly composed of maltose. Since BD liquid is not a standardized product and its utilization value has not been explored, its destination is mainly low-price takeout. This not only affects the industrial benefits, but also is not conducive to the healthy development of the starch sugar industry. Therefore, further exploring and improving the utilization value of BD liquid is of great significance to the development of the starch sugar industry.

[0005] At present, no company has applied BD liquid to the preparation of sports nutrition foods, and the specific efficacy of BD liquid has not been mentioned in relevant studies. Exploring the specific efficacy of BD liquid and its feasibility in sports foods is important for the value-added utilization of the end by-products of starch sugar production. Summary of the invention

[0006] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0007] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0008] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide an application of an oligosaccharide syrup in the preparation of a product for enhancing the athletic performance of an organism.

[0009] In order to solve the above technical problems, the present invention provides the following technical solutions, including: the oligosaccharide syrup reduces the content of metabolites in the body, increases energy reserves, promotes the balance between oxidation and anti-oxidation, alleviates muscle damage, and improves muscle endurance to enhance the body's athletic performance;

[0010] The oligosaccharide syrup improves the body's athletic performance by 2 to 4 times.

[0011] As a preferred solution for using the oligosaccharide syrup of the present invention in preparing products for enhancing body athletic performance, the products include food and health products.

[0012] As a preferred embodiment of the application of the oligosaccharide syrup of the present invention in the preparation of products for enhancing the body's athletic performance, the oligosaccharide syrup is used alone or as an additive in common foods, sports nutrition foods, and health products.

[0013] The use of the oligosaccharide syrup described in the present invention in the preparation of a product that enhances the body's athletic performance is a preferred solution, wherein: the amount of the oligosaccharide syrup added to the product is 10-100%.

[0014] The oligosaccharide syrup described in the present invention is used as a preferred embodiment for preparing a product for enhancing the body's athletic performance, wherein: the enhanced athletic performance refers to prolonged athletic endurance time, increased liver glycogen and muscle glycogen content, and decreased blood lactate and urea nitrogen content.

[0015] The use of the oligosaccharide syrup described in the present invention in the preparation of a product that enhances body athletic performance is a preferred embodiment, wherein: the body athletic performance includes energy metabolism and antioxidant related indicators.

[0016] The purpose of the present invention is to overcome the deficiencies in the prior art and provide an oligosaccharide syrup.

[0017] As a preferred embodiment of the oligosaccharide syrup described in the present invention, the oligosaccharide syrup is a refined BD liquid and an enzyme-treated product thereof.

[0018] The oligosaccharide syrup described as a preferred embodiment of the present invention comprises: the BD liquid comprises 20-30% glucose, 40-50% disaccharide, 20-30% trisaccharide, and 2-10% tetrasaccharide and above.

[0019] As a preferred embodiment of the oligosaccharide syrup described in the present invention, the enzyme is an enzyme preparation that increases the functional components in the BD liquid.

[0020] As a preferred embodiment of the oligosaccharide syrup described in the present invention, the enzyme comprises α-glucoside.

[0021] Beneficial effects of the present invention:

[0022] The effects of oligosaccharide syrup on the body's energy metabolism and anti-oxidation show that it has the ability to relieve sports fatigue and improve endurance, which is specifically manifested in reducing the accumulation of metabolites such as lactic acid and urea nitrogen in serum, increasing the reserves of energy substances such as liver glycogen and muscle glycogen, reducing the level of lipid peroxide malondialdehyde and increasing the activity of superoxide dismutase and glutathione peroxidase, alleviating skeletal muscle damage and improving its strength. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0024] Figure 1 This is the timeline and grouping diagram of the mouse swimming experiment.

[0025] Figure 2 This graph shows the effect of BD solution on the exhaustive swimming time and biochemical indices related to energy metabolism in mice.

[0026] Figure 3 This is a graph showing the effect of BD liquid on antioxidant indicators in physically fatigued mice. DETAILED DESCRIPTION

[0027] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0028] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0029] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor is it a separate or selective embodiment that is mutually exclusive with other embodiments.

[0030] The raw materials used in the present invention are all commonly available in the market unless otherwise specified.

[0031] The ICR mice involved in the embodiments of the present invention were purchased from Sibeifu (Beijing) Biotechnology Co., Ltd.

[0032] The BD liquid and its enzyme-treated product involved in the embodiments of the present invention are:

[0033] BD solution was obtained from Xiangyu Jingu Biochemical Technology Co., Ltd. Its solid content was diluted to 10% with deionized water, and the pH was adjusted to 5.0 with 2M NaOH solution. The solution was kept warm at 55°C for 10 min, and then 150 U / g (dry matter) of α-glucosidase was added. The solution was reacted for 6 h and 30 h under the above conditions. After the reaction, the enzyme was inactivated at high temperature for 30 min. After cooling, the product was purified by centrifugation, decolorization, filtration, and separation to obtain the target product, which was lyophilized into powder. Finally, a 400 mg / mL intragastric solution was accurately weighed and stored at -20°C for later use.

[0034] The relevant biochemical index detection method in the embodiment of the present invention is as follows:

[0035] The biochemical indices included in the examples include blood glucose, serum lactate, serum urea nitrogen, lactate dehydrogenase, creatine kinase, tissue glycogen, malondialdehyde, superoxide dismutase, glutathione peroxidase, etc. The detection kit is from Nanjing Jiancheng Bioengineering Institute, and the detection steps are carried out according to the kit instructions.

[0036] Example 1

[0037] Safety evaluation of chromatographic extract of crystallized glucose mother liquor (BD solution)

[0038] The specific steps are as follows:

[0039] Thirty healthy ICR mice aged 4 weeks were taken, half male and half female, and adapted to the environment for 1 week. They were randomly divided into 2 groups, including a blank control group and a toxic dose group. Acute toxicity test was carried out on the 8th day. The test method was referred to the "limit method" in GB15193.3-2014. The number of mice in the blank control group was set to 10. Half male and half female, each mouse in this group was gavaged with 0.2mL / 10g·BW sterile water. The number of mice in the toxic dose group was set to 20, half male and half female. The toxic dose of this group was 10mg / g·BW, and each mouse was gavaged with 0.2mL / 10g·BW BD solution. Fasting but not water for 4-6h before gavage, refeeding 1-2h after gavage, feeding the mice with basic feed during this period, detecting the food intake, water intake, body weight of the mice at a fixed time every day and observing the status of the mice. If there are symptoms of poisoning and death, the symptoms and the time of death of the dead mice should be recorded in detail, and the autopsy should be performed immediately to record the number of deaths of mice in each dose group. The surviving mice were killed on the 22nd day. Before dissection, the body surface and natural orifices of each group of animals were examined, including the ear cavity, perianal cavity, thoracic cavity, nasal cavity, urethral orifice, pelvic cavity, oral cavity, abdominal cavity, and cranial cavity. After death. During the dissection, the size, appearance, hardness, color, edema, bleeding or other changes of the main organs (liver, kidney, spleen, stomach, lung, heart, brain, etc.) were observed with the naked eye. Then the organs were carefully removed and weighed after removing the blood on the surface with 4°C pre-cooled saline to calculate the organ coefficient (organ / body weight percentage). Subsequently, each organ was placed in liquid nitrogen for quick freezing or immersed in 4% tissue cell fixative for testing. Based on the above-mentioned phenomena and data, the acute toxicity intensity of the chromatographic extract to mice was evaluated, and the LD 50 Acute toxicity classification is also carried out to derive the dose-response (effect) relationship.

[0040] Toxicity observation and median lethal dose (LD 50) The measurement results are as follows:

[0041] Within 14 days after exposure, the mice showed no signs of death, and there were no obvious abnormalities in their movement, secretions, excretions, skin and fur. After the mice were dissected, the brain, heart, liver, spleen, lungs, kidneys and other major organs were observed with the naked eye, and no obvious abnormalities were found. Based on the above toxicity observations, the oral median lethal dose (LD50) of the chromatographic extract in mice 50) The judgment results are shown in Table 1. It can be seen from the table that LD 50 >10mg / g·BW.

[0042] Table 1 BD liquid LD 50 Judgment result

[0043]

[0044] The results of the measurements of mouse body weight, food intake, and water consumption are as follows:

[0045] During the 14-day observation period, the results of the changes in the weight, food intake, and water intake of the mice are shown in Table 2. As can be seen from the table, the weight of the mice in each group showed an overall increasing trend during the observation period. Compared with the control group, there was no significant difference in the weight, food intake, and water intake of the mice in the toxic dose group (p>0.05).

[0046] Table 2 Effects of chromatographic extract on body weight, food intake and water intake of mice

[0047]

[0048]

[0049] Note: The body weight, food intake and water intake of each group are expressed as mean ± standard deviation (body weight n = 10, food intake and water intake n = 2).

[0050] There was no significant difference in the above data

[0051] The results of the mouse organ coefficient determination are as follows:

[0052] The effects of the chromatographic extract on the organ coefficients of the main organs of mice, such as the brain, heart, liver, spleen, lung, and kidney, are shown in Table 3. It can be seen from the table that after oral ingestion of the chromatographic extract and a 14-day observation period, there is no significant difference in the organ coefficients of mice in the toxic dose group compared with the blank control group, which can preliminarily determine that the chromatographic extract has no obvious toxic effect on mouse organs.

[0053] Table 3 Effects of chromatographic raffinate on mouse organ coefficients

[0054]

[0055] Note: The organ ratio of each group is expressed as mean ± standard deviation (n = 10).

[0056] There was no significant difference in the above data

[0057] In general, BD solution has no obvious acute toxic effects.

[0058] Example 2

[0059] Effect of chromatographic extract of crystallized glucose mother liquor (BD solution) on the motor performance of mice

[0060] Mouse swimming experiment timeline and grouping Figure 1 As shown, the specific steps are as follows:

[0061] 48 healthy male ICR mice aged 4 weeks were taken and adapted to the environment for 1 week. They were randomly divided into 6 groups (8 mice in each group): control group (Control), model group (Model), glucose group (GLU) and 3 BD liquid groups (BDL, BDM, BDH). The mice in each group were free to drink water and eat basic feed. During the experiment, the Control group and the Model group were gavaged with 0.2mL / 10g·BW of normal saline; the GLU group was gavaged with glucose solution, and the 3 BD liquid groups were gavaged with 3 BD liquids with different IMO contents. The gavage dose of the GLU group and the BD liquid group was 4mg / g·BW, and the gavage volume was the same as the Control group and the Model group.

[0062] From day 8 to day 35, the Model group, GLU group and the three BD solution groups performed a cycle of 5 days of unweighted swimming training and 2 days of rest.

[0063] On the 36th day, the Model group, GLU group and three BD solution groups were subjected to a weight-loaded swimming (5% of the mouse body weight) exhaustion experiment.

[0064] On the 38th day, the Model group, GLU group and three BD liquid groups were trained in unweighted swimming. After the experiment, the mice in all groups were anesthetized and blood was collected from the eyeballs for the determination of serum lactate, serum urea nitrogen, lactate dehydrogenase and other indicators; then the mice were killed by cervical dislocation, and the colon, liver and hind leg gastrocnemius were dissected out. Part of the colon and gastrocnemius tissues were fixed in 4% paraformaldehyde, and the remaining organ tissues were quickly frozen with liquid nitrogen and stored at -80°C for subsequent experiments. The weighted and unweighted swimming in this experiment were carried out at a water temperature of 25±1°C. The training time of each unweighted swimming was 15 minutes, and the specific swimming time may be adjusted according to the actual state of the mice; the exhaustion criterion for the weighted swimming experiment was that the head (mouth and nose) of the mouse was completely submerged in the water for 6 seconds. After each swimming training, the mice were wiped dry with paper towels and put back in the cage, and they were free to eat and drink.

[0065] The results of the determination of the exhaustive swimming time and biochemical indices related to energy metabolism of mice are as follows:

[0066] The exhausted swimming time of mice in the three BD liquid groups was significantly higher than that in the Model group. The swimming time of mice in the BDL and BDM groups was 9.68 min and 12.83 min, respectively, which were 2.59 times and 3.43 times that of the Model group. Compared with the Glu group, the exhausted swimming time of the BD liquid group was significantly increased.

[0067] The blood glucose levels of mice in the BDM and BDH groups after swimming were significantly higher than those in the Model and Glu groups, which improved the blood glucose levels of mice under stress.

[0068] After strenuous exercise, the levels of metabolites such as lactate and urea nitrogen in the serum of mice increased. Compared with the Model group, the serum lactate level of mice in the BDL group decreased by 19.52%, and the serum urea nitrogen level of mice in the BDM group decreased by 19.21%, both of which were higher than that in the Glu group. There was no significant difference in lactate and urea nitrogen levels among the three BD liquid groups.

[0069] The activities of lactate dehydrogenase and creatine kinase in serum are important markers of muscle tissue damage. Compared with the Control group, the activities of lactate dehydrogenase and creatine kinase in the serum of mice in the Model group were significantly increased. However, compared with the Model group, the activities of both enzymes in the serum of mice in the BD solution intervention group were significantly decreased, among which the activities of lactate dehydrogenase and creatine kinase in the BDH group were decreased by 25.56% and 46.05%, respectively.

[0070] Liver glycogen and muscle glycogen are energy sources during exercise, and both can reflect the body's fatigue level. The results showed that BD solution can improve the glycogen content in mice and restore it to normal levels; compared with the Model group, the liver glycogen content of the BDH group increased by 33.59%, and the muscle glycogen content of the BDM group increased by 34.01%.

[0071] The results of the determination of antioxidant-related biochemical indicators in mice are as follows:

[0072] Figure 2 This is the effect of BD solution on the exhaustive swimming time and biochemical indicators related to energy metabolism in mice. Figure 3 This is the effect of BD liquid on the antioxidant indexes of physically fatigued mice. It can be seen that compared with the Model group, the malondialdehyde content of the BDM group mice showed a downward trend, but there was no significant difference. However, the malondialdehyde levels of the BDL group and the BDH group were significantly reduced. The activities of superoxide dismutase and glutathione peroxidase in the BDL group mice did not increase significantly, but the activities of the two antioxidant enzymes in the BDM group and the BDH group were significantly increased, and the glutathione peroxidase activity was significantly higher than that of the Glu group.

[0073] In summary, BD fluid can improve the body's athletic performance by reducing the accumulation of exercise metabolites, increasing the content of energy substances in the body, and promoting the balance between oxidation and anti-oxidation.

[0074] Example 3

[0075] Oligosaccharide syrup for sports drinks

[0076] The oligosaccharide syrup can be used to prepare sports drinks, and the content of each component in the sports drink is as follows: 11% of the oligosaccharide syrup, 5% of sodium chloride, 2.6% of vitamin C, and 1% of edible flavor per 100 mL. The above components are dissolved in pure water according to the proportion and filtered, sterilized, and canned to obtain the sports drink.

[0077] Example 4

[0078] Oligosaccharide syrup is used to prepare sports energy gel

[0079] The oligosaccharide syrup can be used to prepare sports energy gel, and the content of each component in the energy gel is as follows: 30% of the oligosaccharide syrup, 1% of sodium chloride, 3% of vitamin C, 2% of protein, 1% of xanthan gum, and 1% of gellan gum per 100g. The preparation is carried out according to the conventional method for producing energy gel.

[0080] In summary, the present invention demonstrates the effects of the oligosaccharide syrup on the body's energy metabolism and anti-oxidation, indicating that it has the ability to relieve sports fatigue and improve endurance, which is specifically manifested in reducing the accumulation of metabolites such as lactic acid and urea nitrogen in serum, increasing the reserves of energy substances such as liver glycogen and muscle glycogen, reducing the level of lipid peroxide malondialdehyde and increasing the activities of superoxide dismutase and glutathione peroxidase, relieving skeletal muscle damage and improving its strength.

[0081] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. An application of oligosaccharide syrup in the preparation of a product for enhancing body athletic performance, characterized in that: Including, the oligosaccharide syrup enhances the body's athletic performance by reducing the content of metabolites in the body, increasing energy reserves, promoting the balance between oxidation and anti-oxidation, alleviating muscle damage, and improving muscle endurance; The oligosaccharide syrup enhances the body's athletic performance by 2 to 4 times.

2. The use of the oligosaccharide syrup according to claim 1 in the preparation of a product for enhancing body athletic performance, characterized in that: The products include food and health products.

3. The use of the oligosaccharide syrup according to claim 1 in the preparation of a product for enhancing body athletic performance, characterized in that: The oligosaccharide syrup is used alone or as an additive in common food, sports nutrition food and health care products.

4. The use of the oligosaccharide syrup according to claim 1 in the preparation of a product for enhancing body athletic performance, characterized in that: The oligosaccharide syrup is added in an amount of 10-100% in the product.

5. The use of the oligosaccharide syrup according to claim 1 in the preparation of a product for enhancing body athletic performance, characterized in that: The enhanced athletic performance refers to prolonged athletic endurance time, increased liver glycogen and muscle glycogen levels, and decreased blood lactate and urea nitrogen levels.

6. Use of the oligosaccharide syrup according to claim 1 in the preparation of a product for enhancing body athletic performance, characterized in that: The body movement performance includes energy metabolism and antioxidant related indicators.

7. An oligosaccharide syrup according to any one of claims 1 to 6, characterized in that: The oligosaccharide syrup is a refined BD liquid and an enzyme-treated product thereof.

8. The oligosaccharide syrup according to claim 7, characterized in that: The BD solution comprises 20-30% glucose, 40-50% disaccharide, 20-30% trisaccharide, and 2-10% tetrasaccharide and above.

9. The oligosaccharide syrup according to claim 7, characterized in that: The enzyme is an enzyme preparation for improving the functional components in BD liquid.

10. The oligosaccharide syrup according to claim 9, characterized in that: The enzyme includes α-glucosidase.

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