Cell culture device for gene detection

Abstract

The invention discloses a cell culture device for gene detection, and belongs to the field of gene detection. A cell culture device for gene detection comprises a detection box and a microscope, the detection end of the microscope penetrates into the detection box, the bottom of an inner cavity of the detection box is fixedly connected with a low-speed motor, the top end of the output end of the low-speed motor is fixedly connected with a rotating disc, and three sets of clamping bases are distributed at the top of the rotating disc at equal intervals; a culture dish is inserted into the clamping seat; through rotation of the rotating disc, an upper contact ball and a lower contact ball are in contact extrusion, so that gas in a gas collection box is pushed to repeatedly enter a spring telescopic rod, a clamping seat is pushed to slide back and forth at the top of the rotating disc, and a culture solution in a culture dish and cells are slightly shaken; and nutrient substances are promoted to be uniformly distributed in the culture dish, cells are effectively prevented from settling to the bottom of the culture dish, the respiration of the cells is facilitated, the culture speed of the cells is ensured, and the gene detection efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection, and in particular to a cell culture device for gene detection. Background Art

[0002] Genes are the basic units of heredity, DNA or RNA sequences that carry genetic information. They pass on genetic information to the next generation through replication, and guide the synthesis of proteins to express the genetic information they carry, thereby controlling the expression of traits of individual organisms. Genetic testing is a technology that obtains genetic information by analyzing the DNA of an individual. This information can be used for a variety of purposes, such as diagnosing genetic diseases, assessing disease risks, guiding treatment, and paternity testing. When diagnosing genetic diseases and conducting prenatal screening, it is usually necessary to test whether there are abnormalities in the number and structure of chromosomes. The specific testing steps include sample collection, cell culture, staining, and microscopic observation.

[0003] At present, the existing cell culture devices for gene detection usually adopt static culture when culturing cells, so that the nutrient solution or other growth-required substances in the culture medium are difficult to contact the cells evenly, and the cells may settle to the bottom of the culture medium, resulting in slow growth and reducing the efficiency of gene detection; and the detection end of the microscope may be attached with attachments for various reasons, thereby reducing the imaging quality of the microscope and causing a reduction in the accuracy of the detection results. Therefore, a cell culture device for gene detection is proposed. Summary of the invention

[0004] The purpose of the present invention is to solve the problems in the prior art that cells are difficult to uniformly contact with substances required for growth, resulting in slow growth and reduced gene detection efficiency; and the presence of attachments on the detection end of the microscope will reduce the imaging quality of the microscope, resulting in reduced accuracy of the detection results, and a cell culture device for gene detection is proposed to solve the problems in the prior art that cells are difficult to uniformly contact with substances required for growth, resulting in slow growth and reduced efficiency of gene detection; and the presence of attachments on the detection end of the microscope will reduce the imaging quality of the microscope, resulting in reduced accuracy of the detection results.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A cell culture device for gene detection comprises a detection box and a microscope, wherein the detection end of the microscope penetrates into the detection box, a slow motor is fixedly connected to the bottom of the inner cavity of the detection box, a turntable is fixedly connected to the top of the output end of the slow motor, three groups of card seats are evenly distributed on the top of the turntable, a culture dish is inserted into the card seat, and a card portion for fixing the culture dish is arranged in the card seat, and also comprises: a shaking component, which is arranged in the detection box and is used to slowly shake the culture dish; and a cleaning component, which is arranged in the detection box and is used to adsorb and clean attachments on the microscope lens.

[0007] In order to improve stability, preferably, the clamping part includes a clamping groove, the clamping groove is opened on the inner wall of the clamping seat, a clamping block is slidably connected in the clamping groove, a first spring is fixedly connected between the side wall of the clamping block and the inner wall of the clamping seat, and the top of the clamping block close to the culture dish side is treated with an arc-shaped rounded corner.

[0008] In order to improve the cell growth efficiency, preferably, the shaking assembly includes a triangular groove, which is opened at the top of the turntable, and the three side grooves of the triangular groove extend to the bottom of three groups of clamping seats respectively, and moving blocks are slidably connected in the grooves of the triangular groove, and the top of the moving block is fixedly connected to the bottom of the clamping seat, and the middle of the triangular groove is fixedly connected to an air collecting box, and the side wall of the air collecting box is fixed and connected with a spring telescopic rod, and the three groups of spring telescopic rods extend to the three side grooves of the triangular groove respectively, and the telescopic ends of the spring telescopic rods are fixedly connected to the side walls of the moving block, and the bottom of the turntable is provided with an air pressure part that pushes the telescopic end of the spring telescopic rod to move back and forth.

[0009] Furthermore, the pneumatic part includes a pneumatic box, a pneumatic plate is slidably connected inside the pneumatic box, a second spring is fixedly connected between the top of the pneumatic plate and the inner wall of the pneumatic box, the upper cavity of the pneumatic box is connected to the inner cavity of the air collecting box through an air guide groove, an upper touch ball is fixedly connected to the bottom of the pneumatic plate, and a lower touch ball is fixedly connected to the bottom of the inner cavity of the detection box.

[0010] In order to improve the accuracy of the detection results, preferably, the cleaning assembly includes a ventilation box, on the side wall of which an air suction pipe is fixed and connected, and two groups of the air suction pipes are symmetrically arranged along the center of the detection end of the microscope, and the input ends of the two groups of the air suction pipes are both facing the side wall of the detection end of the microscope, and an exhaust part that generates suction in the air suction pipe is arranged in the detection box.

[0011] Furthermore, the air extraction part includes a transmission shaft, which is rotatably connected to the inner wall of the detection box, and a friction wheel is fixedly connected to the transmission shaft, and the friction wheel rotates in engagement with the side wall of the turntable, and a piston box is fixedly connected in the detection box, a piston groove is provided on the top of the piston box, a lower magnetic plate is slidably connected in the piston groove, a third spring is fixedly connected between the bottom of the lower magnetic plate and the piston groove, an upper magnetic plate is fixedly connected to the bottom of the friction wheel, and the upper magnetic plate and the lower magnetic plate repel each other magnetically, the ventilation box and the piston groove are connected through an air duct, and a one-way valve is provided in the air duct.

[0012] In order to improve the stability of the culture dish, preferably, a sealing cylinder is fixedly connected to the bottom of the detection box, and the sealing cylinder is sleeved on the slow motor. An air guide box is fixedly connected between the sealing cylinder and the inner wall of the detection box, and the air guide box is communicated with the inner cavity of the sealing cylinder. Heat dissipation holes are evenly spaced at the bottom of the detection box located in the sealing cylinder. The air guide box is connected to the piston groove through an exhaust pipe, and a one-way valve and a solenoid valve are respectively provided in the air guide box.

[0013] Furthermore, a sealing ring is rotatably connected to the bottom of the turntable, and the sealing ring is fixedly connected to the inner wall of the detection box. The sealing ring is connected to the inner cavity of the ventilation box through an inflation pipe, and a one-way valve and a solenoid valve are respectively provided in the inflation pipe. An inflation groove is opened in the turntable, and the inflation groove is connected to the clamping groove through a pressurized pipe, and the inflation groove is connected to the inner cavity of the sealing ring. The bottom of the sealing ring is fixed and connected to a pressure relief pipe, and a solenoid valve is provided in the pressure relief pipe.

[0014] In order to improve the accuracy of the test results, preferably, a dye tank is fixedly connected to the top of the test box, an output pipe of the dye tank extends through the test box and is fixedly connected to a drip tray, and a solenoid valve is provided in the output pipe of the dye tank, and a stirring paddle is rotatably connected in the dye tank, and the top end of the stirring paddle is connected to the transmission shaft via a pulley group.

[0015] Preferably, the drip tray and the detection end of the microscope are respectively located directly above the clamping seats on both sides of the rear portion.

[0016] Compared with the prior art, the present invention provides a cell culture device for gene detection, which has the following beneficial effects:

[0017] 1. The cell culture device for gene detection, through the rotation of the turntable, causes contact and extrusion between the upper touch ball and the lower touch ball, thereby pushing the gas in the gas collecting box to repeatedly enter the spring telescopic rod, thereby pushing the card seat to slide back and forth on the top of the turntable, so that the culture fluid and cells in the culture dish have a gentle shaking effect, promote the uniform distribution of nutrients in the culture dish, effectively prevent the cells from settling to the bottom of the culture dish, and at the same time facilitate the respiration of the cells, ensure the cell culture speed, and improve the efficiency of gene detection.

[0018] 2. The cell culture device for gene detection, through the repulsive action of the upper magnetic plate and the lower magnetic plate, combined with the setting of the third spring, enables the suction tube end to continuously generate suction, thereby adsorbing and cleaning the attachments on the microscope detection end, ensuring the imaging quality of the microscope detection end, and further improving the accuracy of the detection results.

[0019] 3. The cell culture device for gene detection compresses the gas discharged from the piston groove into the sealing tube and the clamping groove at different times. Firstly, the heat generated by the slow motor when it is working can be taken out to avoid the heat from having an adverse effect on cell growth and detection. At the same time, the slow motor can also be protected, thereby improving the durability of the slow motor. Secondly, the air pressure in the clamping groove is increased, so that the clamping block and the culture dish fit more closely, further improving the stability of the culture dish.

[0020] 4. The cell culture device for gene detection drives the stirring paddle to rotate in the dye tank through the rotation of the transmission shaft and the transmission action of the pulley group, so as to stir the dye in the dye tank evenly, ensure the use effect of the dye, and improve the accuracy of the final detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the overall structure of a cell culture device for gene detection proposed by the present invention;

[0022] Figure 2 A schematic diagram of the turntable structure of a cell culture device for gene detection proposed by the present invention;

[0023] Figure 3 A schematic diagram of a partial cross-sectional structure of a cell culture device for gene detection proposed by the present invention;

[0024] Figure 4 A partial half-section structural schematic diagram of a cell culture device for gene detection proposed by the present invention;

[0025] Figure 5 A cell culture device for gene detection proposed by the present invention Figure 4 A schematic diagram of the enlarged structure of the middle A area;

[0026] Figure 6 A schematic diagram of a side view and a half-section structure of a cell culture device for gene detection proposed by the present invention;

[0027] Figure 7 A cell culture device for gene detection proposed by the present invention Figure 6 Schematic diagram of the enlarged structure of the middle B area;

[0028] Figure 8 This is a schematic diagram of the internal structure of a stain tank of a cell culture device for gene detection proposed by the present invention.

[0029] In the figure: 1, detection box; 2, microscope; 3, slow motor; 4, turntable; 41, clamping seat; 411, clamping groove; 412, clamping block; 413, first spring; 42, culture dish; 43, sealing ring; 5, triangular groove; 51, moving block; 52, air collecting box; 53, spring telescopic rod; 54, air pressure box; 541, air pressure plate; 542, second spring; 543, air guide groove; 544, upper touch ball; 545, lower touch ball; 6, ventilation box ; 61. Intake pipe; 62. Transmission shaft; 621. Friction wheel; 622. Upper magnetic plate; 63. Piston box; 631. Piston groove; 632. Lower magnetic plate; 633. Third spring; 64. Air guide pipe; 7. Sealing tube; 71. Air guide box; 72. Heat dissipation hole; 73. Exhaust pipe; 74. Inflating pipe; 75. Inflating groove; 751. Pressurizing pipe; 76. Pressure relief pipe; 8. Dye tank; 81. Drip tray; 82. Agitating paddle; 83. Pulley assembly. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0031] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0032] Example:

[0033] Reference Figure 1-Figure 8 A cell culture device for gene detection includes a detection box 1 and a microscope 2. The detection end of the microscope 2 penetrates into the detection box 1. A slow motor 3 is fixedly connected to the bottom of the inner cavity of the detection box 1. A turntable 4 is fixedly connected to the top of the output end of the slow motor 3. Three groups of card seats 41 are evenly distributed on the top of the turntable 4. A culture dish 42 is inserted into the card seat 41, and a card portion for fixing the culture dish 42 is provided in the card seat 41. The device also includes: a shaking component, which is arranged in the detection box 1 and is used to slowly shake the culture dish 42; a cleaning component, which is arranged in the detection box 1 and is used to adsorb and clean the attachments on the lens of the microscope 2.

[0034] Reference Figure 2 , Figure 7The clamping portion includes a clamping groove 411, which is opened on the inner wall of the clamping seat 41, and a clamping block 412 is slidably connected in the clamping groove 411. A first spring 413 is fixedly connected between the side wall of the clamping block 412 and the inner wall of the clamping seat 41, and the top of the clamping block 412 close to the culture dish 42 is treated with an arc-shaped fillet.

[0035] Through the arrangement of the above-mentioned structure, the culture dishes 42 are respectively inserted into the clamping seats 41. During the insertion process, the side walls of the culture dishes 42 will squeeze the clamping blocks 412, so that the clamping blocks 412 compress the first spring 413 and slide into the clamping groove 411. Finally, the rebound effect of the first spring 413 is utilized to make the clamping blocks 412 fit tightly against the side walls of the culture dishes 42, so that the culture dishes 42 are stably clamped in the clamping seats 41, thereby ensuring the stability of the culture dishes 42.

[0036] Reference Figure 2 , Figure 4 , Figure 5 and Figure 7 The shaking assembly includes a triangular groove 5, which is opened at the top of the turntable 4. The three side grooves of the triangular groove 5 extend to the bottom of the three groups of clamping seats 41 respectively. The grooves of the triangular groove 5 are all slidably connected with moving blocks 51. The top of the moving block 51 is fixedly connected to the bottom of the clamping seat 41. The middle of the triangular groove 5 is fixedly connected with an air collecting box 52. The side wall of the air collecting box 52 is fixed and connected with a spring telescopic rod 53. The three groups of spring telescopic rods 53 extend to the three side grooves of the triangular groove 5 respectively, and the telescopic ends of the spring telescopic rods 53 are connected to the moving blocks. 51 is fixedly connected to the side wall, and a pneumatic part is arranged at the bottom of the turntable 4 to push the telescopic end of the spring telescopic rod 53 to move back and forth; the pneumatic part includes a pneumatic box 54, and a pneumatic plate 541 is slidably connected inside the pneumatic box 54, a second spring 542 is fixedly connected between the top of the pneumatic plate 541 and the inner wall of the pneumatic box 54, the upper cavity of the pneumatic box 54 is connected to the inner cavity of the air collecting box 52 through an air guide groove 543, an upper touch ball 544 is fixedly connected to the bottom of the pneumatic plate 541, and a lower touch ball 545 is fixedly connected to the bottom of the inner cavity of the detection box 1.

[0037] By setting the above structure, the slow motor 3 is turned on to drive the turntable 4 to rotate slowly. When the turntable 4 rotates with the upper touch ball 544 to the position in contact with the lower touch ball 545, as the turntable 4 continues to rotate, the upper touch ball 544 will pass over the lower touch ball 545, and in the process of passing over, the upper touch ball 544 will push the air pressure plate 541 to slide upward, thereby compressing the gas in the air pressure box 54, and allowing this part of the gas to enter the air collecting box 52 along the air guide groove 543, and then enter the three sets of spring telescopic rods 53 from the air collecting box 52, thereby pushing the telescopic ends of the spring telescopic rods 53 to extend outward, and then when the upper touch ball 544 passes over the highest point of the lower touch ball 545, the spring telescopic rods 53 and the lower touch ball 545 are connected. Under the rebound action of the second spring 542, the upper touch ball 544 will drive the air pressure plate 541 to gradually move down and reset, so that the gas compressed into the spring telescopic rod 53 can flow back, and so on, so that the spring telescopic rod 53 can push the moving block 51 to slide back and forth with the clamping seat 41 on the top of the turntable 4, so that the culture liquid and cells in the culture dish 42 can be gently shaken, which promotes the uniform distribution of nutrients in the culture dish 42, effectively prevents the cells from settling to the bottom of the culture dish 42, and helps the cells to suspend and grow better. At the same time, it can also increase the contact area between the culture medium and the air, which is beneficial to the respiration of the cells. In summary, the cell culture speed is ensured and the gene detection efficiency is improved.

[0038] Reference Figure 2-Figure 4 The cleaning assembly includes a ventilation box 6, a suction pipe 61 is fixed on the side wall of the ventilation box 6 and connected to the side wall, and two groups of suction pipes 61 are symmetrically arranged along the center of the detection end of the microscope 2. The input ends of the two groups of suction pipes 61 are both facing the side wall of the detection end of the microscope 2, and an exhaust part that generates suction in the suction pipe 61 is arranged in the detection box 1; the exhaust part includes a transmission shaft 62, the transmission shaft 62 is rotatably connected to the inner wall of the detection box 1, and a friction wheel 621 is fixedly connected to the transmission shaft 62, and the friction wheel 621 is connected to the side of the turntable 4. The piston box 63 is fixedly connected to the detection box 1, and a piston groove 631 is provided on the top of the piston box 63. A lower magnetic plate 632 is slidably connected in the piston groove 631. A third spring 633 is fixedly connected between the bottom of the lower magnetic plate 632 and the piston groove 631. An upper magnetic plate 622 is fixedly connected to the bottom of the friction wheel 621. The upper magnetic plate 622 and the lower magnetic plate 632 repel each other magnetically. The ventilation box 6 and the piston groove 631 are connected through an air duct 64, and a one-way valve is arranged in the air duct 64.

[0039] It should be noted that the one-way valve in the air guide pipe 64 can only allow the airflow near the input end of the air intake pipe 61 to enter the piston groove 631 along the air guide pipe 64 .

[0040] By setting the above structure, during the rotation of the turntable 4, the friction force will drive the friction wheel 621 and the transmission shaft 62 to rotate. During the rotation of the friction wheel 621, the repulsion between the upper magnetic plate 622 and the lower magnetic plate 632 will cause the lower magnetic plate 632 to move toward the side of the third spring 633, thereby compressing the gas in the piston groove 631 and discharging the gas in the piston groove 631. When the upper magnetic plate 622 and the lower magnetic plate 632 are separated from the repulsive area, the lower magnetic plate 632 will be reset under the rebound action of the third spring 633, thereby generating suction in the piston groove 631 and opening the one-way valve in the air guide tube 64, so that the gas around the detection end of the microscope 2 is sucked into the piston groove 631 along the suction tube 61, the ventilation box 6 and the air guide tube 64, so as to adsorb and clean the attachments on the detection end of the microscope 2, ensure the imaging quality of the detection end of the microscope 2, and further improve the accuracy of the detection result.

[0041] Reference Figure 3 , Figure 4 , wherein a sealing cylinder 7 is fixedly connected to the bottom of the detection box 1, the sealing cylinder 7 is sleeved on the slow motor 3, an air guide box 71 is fixedly connected between the sealing cylinder 7 and the inner wall of the detection box 1, the air guide box 71 is communicated with the inner cavity of the sealing cylinder 7, and heat dissipation holes 72 are evenly spaced at the bottom of the detection box 1 located in the sealing cylinder 7, the air guide box 71 is connected to the piston groove 631 through an exhaust pipe 73, and a one-way valve and a solenoid valve are respectively arranged in the air guide box 71.

[0042] It should be noted that, in the later stage of the detection, the solenoid valve in the air guide box 71 is in an open state, while the solenoid valve in the pressure relief pipe 76 is in a closed state. In addition, the one-way valve in the air guide box 71 can only allow the gas in the piston groove 631 to enter the sealing tube 7.

[0043] Through the arrangement of the above structure, the gas compressed by the downward movement of the lower magnetic plate 632 in the piston groove 631 will open the one-way valve in the air guide box 71, so that this part of the gas enters the sealing tube 7 along the exhaust pipe 73 and the air guide box 71, and is finally discharged to the outside through the heat dissipation hole 72 at the bottom of the inner cavity of the sealing tube 7, thereby bringing out the heat generated by the slow-speed motor 3 when it is working, avoiding the adverse effects of heat on cell growth and detection, and also realizing the protection of the slow-speed motor 3, thereby improving the durability of the slow-speed motor 3.

[0044] Reference Figure 3-Figure 5, wherein, a sealing ring 43 is rotatably connected to the bottom of the turntable 4, and the sealing ring 43 is fixedly connected to the inner wall of the detection box 1. The sealing ring 43 is connected to the inner cavity of the air guide box 71 through an inflation pipe 74, and a one-way valve and a solenoid valve are respectively arranged in the inflation pipe 74. An inflation groove 75 is opened in the turntable 4, and the inflation groove 75 is connected to the clamping groove 411 through a pressurizing pipe 751, and the inflation groove 75 is connected to the inner cavity of the sealing ring 43. The bottom of the sealing ring 43 is fixed and connected to a pressure relief pipe 76, and a solenoid valve is arranged in the pressure relief pipe 76.

[0045] It should be noted that, in the early stage of detection, the solenoid valve of the inflation tube 74 is in an open state, while the solenoid valve in the air guide box 71 is in a closed state. In addition, the one-way valve in the inflation tube 74 can only allow the gas in the piston groove 631 to enter the sealing ring 43.

[0046] Through the arrangement of the above structure, the gas compressed by the downward movement of the lower magnetic plate 632 in the piston groove 631 will open the one-way valve in the inflation tube 74, so that this part of the gas enters the sealing ring 43 along the exhaust pipe 73, the air guide box 71 and the inflation tube 74, and then enters the inflation groove 75 from the sealing ring 43, and finally enters the clamping groove 411 along the pressurizing tube 751, thereby increasing the air pressure in the clamping groove 411, making the clamping block 412 and the culture dish 42 fit more closely, and further improving the stability of the culture dish 42; finally, after the detection is completed, the solenoid valve in the pressure relief tube 76 can be opened to discharge the increased air pressure in the clamping groove 411, so that the culture dish 42 can be taken out.

[0047] Reference Figure 1 , Figure 4 and Figure 8 , wherein a dye tank 8 is fixedly connected to the top of the detection box 1, an output pipe of the dye tank 8 extends through the detection box 1 and is fixedly connected to a drip tray 81, and an electromagnetic valve is provided in the output pipe of the dye tank 8, a stirring paddle 82 is rotatably connected in the dye tank 8, and the top end of the stirring paddle 82 is connected to the transmission shaft 62 through a pulley set 83; the drip tray 81 and the detection end of the microscope 2 are respectively located directly above the clamping seats 41 on both sides of the rear.

[0048] Through the arrangement of the above structure, during the rotation of the transmission shaft 62, the transmission action of the pulley set 83 will drive the stirring paddle 82 to rotate in the dye tank 8, thereby stirring the dye in the dye tank 8, thereby ensuring the use effect of the dye and improving the accuracy of the final detection.

[0049] Reference Figure 1-Figure 8In the present invention, when in use, the cells separated from the test sample are inoculated into the culture dish 42 prepared with the culture fluid, and three groups of culture dishes 42 are set according to the same conditions, and then the three groups of culture dishes 42 are respectively inserted into the three groups of clamping seats 41. During the insertion process, the side wall of the culture dish 42 will squeeze the clamping block 412, so that the clamping block 412 compresses the first spring 413 and slides into the clamping groove 411. Finally, the rebound effect of the first spring 413 is utilized to make the clamping block 412 fit tightly on the side wall of the culture dish 42, so that the culture dish 42 is stably clamped in the clamping seat 41, ensuring the stability of the culture dish 42, and then the detection box 1 is closed.

[0050] During the cell culture process, the slow motor 3 is turned on to drive the turntable 4 to rotate slowly. When the turntable 4 rotates with the upper touch ball 544 to the position in contact with the lower touch ball 545, as the turntable 4 continues to rotate, the upper touch ball 544 will pass over the lower touch ball 545. In the process of passing over, the upper touch ball 544 will push the air pressure plate 541 to slide upward, thereby compressing the gas in the air pressure box 54, and allowing this part of the gas to enter the air collecting box 52 along the air guide groove 543, and then enter the three sets of spring telescopic rods 53 from the air collecting box 52, thereby pushing the telescopic end of the spring telescopic rod 53 to extend outward. Subsequently, when the upper touch ball 544 passes over the highest point of the lower touch ball 545, the spring telescopic plate 541 is pushed upward to compress the gas in the air pressure box 54. Under the rebound action of the rod 53 and the second spring 542, the upper touch ball 544 will drive the air pressure plate 541 to gradually move down and reset, so that the gas compressed into the spring telescopic rod 53 can flow back, and so on, so that the spring telescopic rod 53 pushes the moving block 51 to slide back and forth on the top of the turntable 4 with the clamping seat 41, so that the culture liquid and cells in the culture dish 42 can be gently shaken, which promotes the uniform distribution of nutrients in the culture dish 42, effectively prevents the cells from settling to the bottom of the culture dish 42, and helps the cells to suspend and grow better. At the same time, it can also increase the contact area between the culture medium and the air, which is beneficial to the respiration of the cells. In summary, the culture efficiency of the cells is effectively improved.

[0051] During the rotation of the turntable 4, the microscope 2 will continue to detect the cell growth conditions in the three groups of culture dishes 42. When the cells in the culture dishes 42 are cultured to the metaphase, the culture dishes 42 are rotated to the bottom of the drip tray 81 as needed, and the solenoid valve in the output tube of the dye tank 8 is opened to allow the dye to drip into the culture dishes 42 to color the chromosomes of the cells. Then, the turntable 4 is continued to be rotated, and the culture dishes 42 are shaken for a period of time, and then the culture dishes 42 are moved one by one to the bottom of the detection end of the microscope 2, so as to observe the morphology and number of chromosomes through the microscope 2, take photos and perform karyotype analysis, so as to complete the detection of abnormal chromosome number and structure. At the same time, the three groups of data can be compared, which effectively improves the accuracy of the test results.

[0052] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A cell culture device for gene detection, comprising a detection box (1) and a microscope (2), wherein the detection end of the microscope (2) penetrates into the detection box (1), characterized in that: The bottom of the inner cavity of the detection box (1) is fixedly connected to a slow motor (3), the top of the output end of the slow motor (3) is fixedly connected to a turntable (4), the top of the turntable (4) is evenly spaced with three groups of clamping seats (41), a culture dish (42) is inserted into the clamping seat (41), and a clamping portion for fixing the culture dish (42) is provided in the clamping seat (41), and further comprises: A shaking assembly, the shaking assembly being arranged in the detection box (1) and being used for slowly shaking the culture dish (42); A cleaning component is arranged in the detection box (1), and is used to absorb and clean the attachments on the lens of the microscope (2).

2. A cell culture device for gene detection according to claim 1, characterized in that: The clamping portion comprises a clamping groove (411), the clamping groove (411) is formed on the inner wall of the clamping seat (41), a clamping block (412) is slidably connected in the clamping groove (411), a first spring (413) is fixedly connected between the side wall of the clamping block (412) and the inner wall of the clamping seat (41), and the top of the clamping block (412) close to the culture dish (42) is processed with an arc-shaped fillet.

3. A cell culture device for gene detection according to claim 1, characterized in that: The shaking assembly comprises a triangular groove (5), wherein the triangular groove (5) is provided at the top of the turntable (4), and the three side grooves of the triangular groove (5) respectively extend to the bottom of three groups of clamping seats (41), and the grooves of the triangular groove (5) are all slidably connected with moving blocks (51), and the top of the moving block (51) is fixedly connected to the bottom of the clamping seat (41), and the middle of the triangular groove (5) is fixedly connected with an air collecting box (52), and the side wall of the air collecting box (52) is fixed and connected with a spring telescopic rod (53), and the three groups of the spring telescopic rods (53) respectively extend into the three side grooves of the triangular groove (5), and the telescopic ends of the spring telescopic rods (53) are fixedly connected to the side walls of the moving blocks (51), and the bottom of the turntable (4) is provided with a pneumatic part for pushing the telescopic ends of the spring telescopic rods (53) to move back and forth.

4. A cell culture device for gene detection according to claim 3, characterized in that: The pneumatic part comprises a pneumatic box (54), a pneumatic plate (541) is slidably connected inside the pneumatic box (54), a second spring (542) is fixedly connected between the top of the pneumatic plate (541) and the inner wall of the pneumatic box (54), the upper cavity of the pneumatic box (54) and the inner cavity of the air collecting box (52) are connected via an air guide groove (543), an upper touch ball (544) is fixedly connected to the bottom of the pneumatic plate (541), and a lower touch ball (545) is fixedly connected to the bottom of the inner cavity of the detection box (1).

5. A cell culture device for gene detection according to claim 2, characterized in that: The cleaning assembly comprises a ventilation box (6), a side wall of the ventilation box (6) is fixed with an air suction pipe (61) in communication with the side wall, two groups of the air suction pipes (61) are symmetrically arranged along the center of the detection end of the microscope (2), the input ends of the two groups of the air suction pipes (61) are both oriented toward the side wall of the detection end of the microscope (2), and an exhaust part for generating suction in the air suction pipes (61) is arranged in the detection box (1).

6. A cell culture device for gene detection according to claim 5, characterized in that: The air extraction part comprises a transmission shaft (62), the transmission shaft (62) is rotatably connected to the inner wall of the detection box (1), a friction wheel (621) is fixedly connected to the transmission shaft (62), the friction wheel (621) rotates in contact with the side wall of the turntable (4), a piston box (63) is fixedly connected inside the detection box (1), a piston groove (631) is provided on the top of the piston box (63), a lower magnetic plate (632) is slidably connected inside the piston groove (631), a third spring (633) is fixedly connected between the bottom of the lower magnetic plate (632) and the piston groove (631), an upper magnetic plate (622) is fixedly connected to the bottom of the friction wheel (621), the upper magnetic plate (622) and the lower magnetic plate (632) repel each other magnetically, the ventilation box (6) and the piston groove (631) are connected via an air guide pipe (64), and a one-way valve is provided inside the air guide pipe (64).

7. A cell culture device for gene detection according to claim 6, characterized in that: A sealing cylinder (7) is fixedly connected to the bottom of the detection box (1), and the sealing cylinder (7) is sleeved on the slow motor (3). An air guide box (71) is fixedly connected between the sealing cylinder (7) and the inner wall of the detection box (1). The air guide box (71) is communicated with the inner cavity of the sealing cylinder (7). Heat dissipation holes (72) are arranged at equal intervals on the bottom of the detection box (1) located in the sealing cylinder (7). The air guide box (71) is communicated with the piston groove (631) through an exhaust pipe (73), and a one-way valve and a solenoid valve are respectively arranged in the air guide box (71).

8. A cell culture device for gene detection according to claim 7, characterized in that: The bottom of the turntable (4) is rotatably connected to a sealing ring (43), the sealing ring (43) is fixedly connected to the inner wall of the detection box (1), the sealing ring (43) is connected to the inner cavity of the air guide box (71) through an air charging pipe (74), a one-way valve and an electromagnetic valve are respectively arranged in the air charging pipe (74), an air charging groove (75) is provided in the turntable (4), the air charging groove (75) is connected to the clamping groove (411) through a pressurizing pipe (751), and the air charging groove (75) is connected to the inner cavity of the sealing ring (43), the bottom of the sealing ring (43) is fixed and connected to a pressure relief pipe (76), and a solenoid valve is arranged in the pressure relief pipe (76).

9. The cell culture device for gene detection according to claim 5, characterized in that: The top of the detection box (1) is fixedly connected to a dye tank (8); an output pipe of the dye tank (8) extends through the detection box (1) and is fixedly connected to a drip tray (81); an electromagnetic valve is arranged in the output pipe of the dye tank (8); a stirring paddle (82) is rotatably connected in the dye tank (8); and the top end of the stirring paddle (82) is connected to a transmission shaft (62) via a pulley group (83).

10. A cell culture device for gene detection according to claim 9, characterized in that: The dropper plate (81) and the detection end of the microscope (2) are respectively located directly above the clamping seats (41) on both sides of the rear portion.

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