Hermetia illucens protein extraction device

Abstract

The invention relates to the technical field of hermetia illucens protein extraction equipment, and discloses a hermetia illucens protein extraction device.A storage device comprises a control center, the control center is fixedly connected to the front end surface of the storage device, the top of the storage device is fixedly connected with a first electric rotary table, and the first electric rotary table is electrically connected with the control center; the top output end of the first electric rotary table is fixedly connected with a storage bin, normal operation of the device is guaranteed, the adjusting device is arranged to be an electric push rod, the adjusting device comprises a through rod which is fixedly connected to the top output end of the adjusting device, and the end, away from the adjusting device, of the through rod is fixedly connected with a connecting rod. The top of the connecting rod is fixedly connected with a second electric rotary table, the left output end of the second electric rotary table is fixedly connected with a purification device, and the device has the advantages that the purification quality of the device and the extraction efficiency of protein are improved, and a user can use the device conveniently.

Description

Technical Field

[0001] The present invention relates to the technical field of black soldier fly protein extraction equipment, and particularly to a black soldier fly protein extraction device. Background Art

[0002] Protein purification refers to a biochemical technique that utilizes the inherent similarities and differences among different proteins to remove non-protein contaminants by taking advantage of the similarities among various proteins, and to purify the target protein from other proteins by exploiting the differences among the proteins. Protein purification devices are often used to extract black soldier fly protein in the manufacture of biopharmaceuticals, genetically engineered drugs, and vaccines. The quality of the extracted black soldier fly protein is closely related to the manufacture of biopharmaceuticals, genetically engineered drugs, and vaccines.

[0003] Publication No.: CN202222663837 discloses a protein purification device, which is characterized in that it includes a fermentation tank. The fermentation tank is connected to the bottom of an ultrasonic crushing device through a first liquid pump. Below the liquid outlet at the upper part of the ultrasonic crushing device is a continuous filtration device. The continuous filtration device is connected to a buffer tank. The buffer tank is respectively connected to a number of continuous purification mechanisms through a number of filtered stock solution pipes. A second liquid pump is installed on the filtered stock solution pipes. The continuous purification mechanism includes a first rotating device. The first rotating device is connected to a porous resin column frame. Along the circumferential direction, an adsorption station seat hole, an elution station seat hole, and a cleaning station seat hole are sequentially connected to the porous resin column frame. Porous resin adsorption columns are fixed on the adsorption station seat hole, the elution station seat hole, and the cleaning station seat hole. An elution liquid pipe is installed above the elution station seat hole. The elution liquid pipe is connected to an elution liquid tank above. A cleaning liquid pipe is installed above the cleaning station seat hole. The cleaning liquid pipe is connected to a deionized water tank. Solenoid valves are installed on both the elution liquid pipe and the cleaning liquid pipe. Above the adsorption station seat hole is the filtered stock solution pipe. This device realizes the continuous automation of cell crushing, filtration, protein adsorption, elution, and cleaning of the adsorption column in protein production, improving the efficiency of protein purification. However, in actual use, when this device and existing equipment are used to extract and purify black soldier fly protein, the adsorption method is likely to adsorb both black soldier fly protein and solid-phase substances at the same time, while the direct scraping method is likely to scrape out liquid-phase substances at the same time, resulting in a reduction in the actual purification quality of the device. There is further room for improvement in the extraction and purification quality of black soldier fly protein by existing equipment. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the present invention provides a black soldier fly protein extraction device, which has the advantages of improving the purification quality of the device and the extraction efficiency of protein, and being convenient for users to use.

[0005] To achieve the above object, the present invention provides the following technical solution: A black soldier fly protein extraction device, comprising: a mounting base plate, a device main body, a storage device, a control center, a first electric turntable, a storage bin, an adjusting device, a connecting rod, a second electric turntable, a purification device, an opening and closing door, a first rotation driving assembly, a first rotating shaft, a linear driving assembly, an output rod, an adjusting disc, a first gear disc, a first shaft rod, a second gear disc, a second shaft rod, a stirring rod, a first bevel gear, a second bevel gear, a fixing rod, a stirring plate, an adsorption plate, a rotating rod, a chuck, a separation chamber, a separation bottom plate, a separation groove, a clamping plate, a partition plate, a third shaft rod, a discharge plate, a toothed ring, a movable groove, a clamping groove, a movable plate, a mounting member, a rotating member, a second rotation driving assembly, a second rotating shaft, a third gear disc, a third bevel gear, a fourth shaft rod, a fourth bevel gear, an output shaft, a fan blade, a treatment chamber.

[0006] The positions and connection relationships of the above structures are as follows: A method for extracting black soldier fly protein, comprising a mounting base plate and a device main body for supporting the black soldier fly protein extraction device. The device main body includes a storage device for loading the black soldier fly protein mixture, an adjusting device for height adjustment, and a purification device for purifying and extracting black soldier fly protein, wherein: Crushing: The black soldier fly protein extraction device is externally connected to a crushing device and a liquid chromatographic column. The crushing device crushes a plurality of black soldier flies to form a black soldier fly protein mixture. At this time, the protein mixture is composed of the black soldier fly protein to be extracted and other proteins. The crushing device can be composed of a speed regulating motor, a reducer, a grinding disc, a grinding medium, a feeding device, a discharging device, a cooling device, and a control system; Feeding: The black soldier fly protein mixture formed by crushing through the crushing device is fed into the storage device, and an organic solvent is added to the inside of the storage device to be mixed with the black soldier fly protein mixture, so that the black soldier fly protein mixture is stratified. At this time, the black soldier fly protein to be extracted is in the middle of the organic solvent and other proteins; Separation and extraction: The adjusting device is turned on. The adjusting device moves the purification device to the storage device. Then the purification device is turned on to separate and extract the black soldier fly protein in the middle of the organic solvent and other proteins; Secondary separation: The purification device is turned on to discharge some of the remaining proteins that are adhered together to avoid reducing the extraction and purification quality of the black soldier fly protein. Then the extracted black soldier fly protein is transported into the liquid chromatographic column for purification, and the purified black soldier fly protein is washed with an eluent to obtain a single black soldier fly protein.

[0007] A protein extraction device for black soldier flies, adopting the proposed protein extraction method for black soldier flies. The storage device includes a control center, which is fixedly connected to the front surface of the storage device. A first electric turntable is fixedly connected to the top of the storage device. The first electric turntable is electrically connected to the control center. A storage bin is fixedly connected to the top output end of the first electric turntable to ensure the normal operation of the device.

[0008] Preferably, the adjusting device is set as an electric push rod. The adjusting device includes a through rod, which is fixedly connected to the top output end of the adjusting device. One end of the through rod away from the adjusting device is fixedly connected to a connecting rod. A second electric turntable is fixedly connected to the top of the connecting rod. A purification device is fixedly connected to the left output end of the second electric turntable to ensure the normal operation of the device.

[0009] Preferably, the purification device includes an opening and closing door, which is rotatably connected to the front surface of the purification device. A first rotary drive assembly is fixedly connected to the left side of the inner wall at the top of the purification device. A first rotating shaft is fixedly connected to the bottom output end of the first rotary drive assembly. A linear drive assembly is fixedly connected to the end of the first rotating shaft away from the first rotary drive assembly. The linear drive assembly is set as a hydraulic cylinder. An output rod is differentially connected to the bottom output end of the linear drive assembly to ensure the normal operation of the device.

[0010] Preferably, the purification device further includes an adjusting disk, which is arranged inside the purification device. The output rod passes through the adjusting disk and extends to the outside of the bottom of the adjusting disk. A first gear disk is fixedly connected to the outer surface of the part of the output rod inside the adjusting disk. A first shaft rod is rotatably connected to both the left and right sides of the inner wall at the bottom of the adjusting disk. A second gear disk is fixedly connected to the center of the outer surface of the first shaft rod. A number of engaging teeth are fixedly connected to the inner wall of the adjusting disk near the second gear disk. The second gear disk is engaged and connected inside the adjusting disk through the engaging teeth to ensure the normal operation of the device.

[0011] Preferably, the purification device further includes a stirring rod, which is fixedly connected to the bottom of the adjusting disk. The extended part of the first shaft rod penetrates into the inside of the stirring rod. A second shaft rod is fixedly connected to the bottom of the part of the first shaft rod inside the stirring rod. A first bevel gear is fixedly connected to the bottom of the second shaft rod. A second bevel gear is meshed and connected to the right side of the first bevel gear. A fixing rod is fixedly connected to the end of the second bevel gear away from the first bevel gear. The fixing rod passes through the stirring rod and extends to the outside of the right end of the stirring rod. A stirring plate is fixedly connected to the extended part of the fixing rod. Adsorption plates are fixedly connected to both the top and bottom of the stirring plate. A rotating rod is fixedly connected to the bottom of the first bevel gear. The rotating rod passes through the stirring rod and extends to the outside of the bottom of the stirring rod. A chuck is fixedly connected to the extended part of the rotating rod to ensure the normal operation of the device.

[0012] Preferably, the purification device further includes a separation chamber, which is arranged on the outer surface of the stirring rod. A separation bottom plate is fixedly connected to the bottom of the separation chamber. An isolation plate is arranged inside the separation bottom plate. A clamping plate adapted to the chuck is fixedly connected to the top of the isolation plate. The clamping plate penetrates through the separation bottom plate and extends to the outside of the top of the separation bottom plate. A third shaft rod is fixedly connected to the bottom of the isolation plate. The third shaft rod is rotatably connected to the inner wall of the bottom side of the separation bottom plate. Separation grooves are formed in the top, bottom and inside of the isolation plate of the separation bottom plate to ensure the normal operation of the device.

[0013] Preferably, the purification device further includes a discharge plate, which is fixedly connected to the top of the separation chamber. A plurality of first filter holes are formed inside the discharge plate. Two movable grooves are formed at the top of the discharge plate. A plurality of clamping grooves are arranged at one end of the two movable grooves away from their symmetry plane. The clamping grooves are formed at the top of the discharge plate. A toothed ring is fixedly connected to the outer surface of the discharge plate to ensure the normal operation of the device.

[0014] Preferably, the purification device further includes a movable plate, which is rotatably connected inside the discharge plate. A plurality of second filter holes corresponding to the size and position of the first filter holes are formed inside the movable plate. One end of the movable plate close to the movable groove is fixedly connected with a mounting member. The two mounting members extend to the outside of the top of the movable plate through the movable groove. Rotating members are rotatably connected to the outer surfaces of the two mounting members. A clamping rod adapted to the clamping groove is fixedly connected to the bottom of the rotating member. The top part of the stirring rod is rotatably connected inside the discharge plate and the movable plate. The bottom of the adjusting disc is rotatably connected to the top of the discharge plate to ensure the normal operation of the device.

[0015] Preferably, the purification device further includes a second rotation driving assembly, which is fixedly connected to the right side of the inner wall of the top of the purification device. The second rotation driving assembly is set as a driving motor. A second rotating shaft is fixedly connected to the bottom output end of the second rotation driving assembly. A third toothed disc is fixedly connected to the outer surface of the second rotating shaft and the third toothed disc is meshed with the toothed ring. A processing chamber is arranged at the bottom of the second rotating shaft. The bottom of the processing chamber is fixedly connected to the inner wall of the bottom side of the purification device. The second rotating shaft penetrates and extends into the inside of the processing chamber and a third bevel gear is fixedly connected to the extending part of the second rotating shaft. A fourth bevel gear is meshed with the left side surface of the third bevel gear. A fourth shaft rod is fixedly connected to the right end surface of the fourth bevel gear. The other end of the fourth shaft rod is rotatably connected to the inner wall of the right end of the processing chamber. An output shaft is fixedly connected to the left end surface of the fourth bevel gear. A fan blade is fixedly connected to the outer surface of the output shaft. A heater is fixedly connected to the inside of the processing chamber. An air duct is formed at one end of the processing chamber close to the separation chamber to ensure the normal operation of the device.

[0016] Beneficial effects 1. The black soldier fly protein extraction device can avoid the extraction of organic solvents and black soldier fly proteins together by turning on the purification device, further improving the purification and extraction efficiency of the device. At the same time, after the extraction is completed, the device can stagger the separation grooves at the separation bottom plate and the isolation plate in the same way as above. At this time, a closed space is formed again inside the separation chamber, thus preventing a large amount of other proteins from being extracted and mixed with the black soldier fly proteins, greatly reducing the purification and extraction quality of the device, improving the extraction efficiency and purification and extraction quality of the device for black soldier fly proteins, and facilitating user use.

[0017] 2. The black soldier fly protein extraction device can perform centrifugation by turning on the purification device to keep a single black soldier fly protein in the separation chamber, avoiding the extraction and mixing of other mixed liquids with the black soldier fly proteins, thus greatly reducing the purification and extraction quality of the device, improving the extraction efficiency and purification and extraction quality of the device for black soldier fly proteins, and facilitating user use.

[0018] 3. The black soldier fly protein extraction device can heat the black soldier fly proteins by turning on the purification device and heating the separation chamber during centrifugation, enabling them to be separated from other mixtures faster during centrifugation, improving the extraction efficiency and purification and extraction quality of the device for black soldier fly proteins, and facilitating user use. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic external structure diagram of a black soldier fly protein extraction device of the present invention; Figure 2 is a schematic external structure diagram of the purification device of a black soldier fly protein extraction device of the present invention; Figure 3 is a schematic internal structure diagram of the purification device of a black soldier fly protein extraction device of the present invention; Figure 4 is a schematic structure diagram of the first rotation drive assembly of a black soldier fly protein extraction device of the present invention; Figure 5 is a schematic internal structure diagram of the adjustment disk of a black soldier fly protein extraction device of the present invention; Figure 6 is a schematic internal structure diagram of the stirring rod of a black soldier fly protein extraction device of the present invention; Figure 7 is a schematic internal structure diagram of the separation chamber of a black soldier fly protein extraction device of the present invention; Figure 8 is a schematic internal structure diagram of the separation bottom plate of a black soldier fly protein extraction device of the present invention; Figure 9 is a schematic internal structure diagram of the discharge plate of a black soldier fly protein extraction device of the present invention; Figure 10Schematic diagram of the internal structure of the processing chamber of a protein extraction device for black soldier fly according to the present invention.

[0020] In the figure: 1, mounting base plate; 2, equipment main body; 3, storage device; 30, control center; 31, first electric turntable; 32, storage bin; 4, adjusting device; 40, connecting rod; 41, second electric turntable; 5, purification device; 50, opening and closing door; 51, first rotation drive assembly; 510, first rotating shaft; 511, linear drive assembly; 512, output rod; 52, adjusting disc; 520, first gear disc; 521, first shaft rod; 522, second gear disc; 523, second shaft rod; 53, stirring rod; 530, first bevel gear; 531, second bevel gear; 532, fixed rod; 533, stirring plate; 534, adsorption plate; 535, rotating rod; 536, chuck; 54, separation chamber; 540, separation bottom plate; 541, separation groove; 542, clamping plate; 543, partition plate; 544, third shaft rod; 55, discharge plate; 550, tooth ring; 551, movable groove; 552, clamping groove; 553, movable plate; 554, mounting part; 555, rotating part; 56, second rotation drive assembly; 560, second rotating shaft; 561, third gear disc; 562, third bevel gear; 563, fourth shaft rod; 564, fourth bevel gear; 565, output shaft; 566, fan blade; 567, processing chamber. Detailed implementation manners

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0022] Embodiment 1 Please refer to Figures 1 to 10 , an extraction method for extracting black soldier fly protein, including a mounting base plate 1 for supporting the black soldier fly protein extraction equipment and an equipment main body 2. The equipment main body 2 includes a storage device 3 for loading the black soldier fly protein mixture, an adjusting device 4 for height adjustment, and a purification device 5 for purifying and extracting black soldier fly protein, and the following: Crushing: The black soldier fly protein extraction equipment is externally connected to a crushing equipment and a liquid chromatographic column. The crushing equipment crushes a number of black soldier flies to form a black soldier fly protein mixture. At this time, the protein mixture is composed of the black soldier fly protein to be extracted and other proteins. The crushing equipment can be composed of a speed regulating motor, a reducer, a grinding disc, grinding media, a feeding device, a discharging device, a cooling device, and a control system; Feed the black soldier fly protein mixture formed by crushing through a crushing device into a storage device 3, and add an organic solvent into the storage device 3 to mix with the black soldier fly protein mixture, causing the black soldier fly protein mixture to layer. At this time, the black soldier fly protein to be extracted is in the middle of the organic solvent and the remaining proteins. Separation and extraction: Turn on the adjustment device 4. The adjustment device 4 moves the purification device 5 to the storage device 3, and then turns on the purification device 5 to separate and extract the black soldier fly protein in the middle of the organic solvent and the remaining proteins. Secondary separation: Turn on the purification device 5 to discharge the remaining partially adhered proteins together to avoid reducing the extraction and purification quality of the black soldier fly protein. Then, convey the extracted black soldier fly protein into a liquid chromatography column for purification, and wash the purified black soldier fly protein with an eluent to obtain a single black soldier fly protein.

[0023] Embodiment 2 Please refer to Figures 1 to 10 , on the basis of Embodiment 1, further, a black soldier fly protein extraction device adopts the above-mentioned proposed black soldier fly protein extraction method. The storage device 3 includes a control center 30, which is fixedly connected to the front surface of the storage device 3. A first electric turntable 31 is fixedly connected to the top of the storage device 3. The first electric turntable 31 is electrically connected to the control center 30. A storage bin 32 is fixedly connected to the top output end of the first electric turntable 31 to ensure the normal operation of the device.

[0024] The adjustment device 4 is set as an electric push rod. The adjustment device 4 includes a through rod, which is fixedly connected to the top output end of the adjustment device 4. One end of the through rod away from the adjustment device 4 is fixedly connected to a connecting rod 40. A second electric turntable 41 is fixedly connected to the top of the connecting rod 40. A purification device 5 is fixedly connected to the left end output end of the second electric turntable 41 to ensure the normal operation of the device.

[0025] The purification device 5 includes a hinged door 50, which is rotatably connected to the front surface of the purification device 5. A first rotary drive assembly 51 is fixedly connected to the left side of the top inner wall of the purification device 5. A first rotating shaft 510 is fixedly connected to the bottom output end of the first rotary drive assembly 51. A linear drive assembly 511 is fixedly connected to the end of the first rotating shaft 510 away from the first rotary drive assembly 51. The linear drive assembly 511 is set as a hydraulic cylinder. An output rod 512 is differentially connected to the bottom output end of the linear drive assembly 511 to ensure the normal operation of the device.

[0026] The purification device 5 further includes an adjustment disc 52, which is arranged inside the purification device 5. The output rod 512 passes through the adjustment disc 52 and extends to the outside of the bottom of the adjustment disc 52. A first gear disc 520 is fixedly connected to the outer surface of the part of the output rod 512 inside the adjustment disc 52. On the left and right sides of the inner wall of the bottom side of the adjustment disc 52, a first shaft rod 521 is rotatably connected. At the center of the outer surface of the first shaft rod 521, a second gear disc 522 is fixedly connected. A plurality of engaging teeth are fixedly connected to the inner wall of the adjustment disc 52 near the second gear disc 522. The second gear disc 522 is meshed and connected inside the adjustment disc 52 through the engaging teeth to ensure the normal operation of the device.

[0027] The purification device 5 further includes a stirring rod 53, which is fixedly connected to the bottom of the adjustment disc 52. The extended part of the first shaft rod 521 penetrates into the inside of the stirring rod 53. At the bottom of the part of the first shaft rod 521 inside the stirring rod 53, a second shaft rod 523 is fixedly connected. At the bottom of the second shaft rod 523, a first bevel gear 530 is fixedly connected. On the right side of the first bevel gear 530, a second bevel gear 531 is meshed and connected. At the end of the second bevel gear 531 away from the first bevel gear 530, a fixing rod 532 is fixedly connected. The fixing rod 532 passes through the stirring rod 53 and extends to the outside of the right end of the stirring rod 53. At the extended part of the fixing rod 532, a stirring plate 533 is fixedly connected. Adsorption plates 534 are fixedly connected to the top and bottom of the stirring plate 533. At the bottom of the first bevel gear 530, a rotating rod 535 is fixedly connected. The rotating rod 535 passes through the stirring rod 53 and extends to the outside of the bottom of the stirring rod 53. At the extended part of the rotating rod 535, a chuck 536 is fixedly connected to ensure the normal operation of the device.

[0028] The purification device 5 further includes a separation chamber 54, which is arranged on the outer surface of the stirring rod 53. The bottom of the separation chamber 54 is fixedly connected with a separation bottom plate 540. An isolation plate 543 is arranged inside the separation bottom plate 540. At the top of the isolation plate 543, a clamping plate 542 adapted to the chuck 536 is fixedly connected. The clamping plate 542 passes through the separation bottom plate 540 and extends to the outside of the top of the separation bottom plate 540. At the bottom of the isolation plate 543, a third shaft rod 544 is fixedly connected. The third shaft rod 544 is rotatably connected to the inner wall of the bottom side of the separation bottom plate 540. Separation grooves 541 are opened at the top, bottom of the separation bottom plate 540 and inside the isolation plate 543 to ensure the normal operation of the device.

[0029] The purification device 5 further includes a discharge plate 55, which is fixedly connected to the top of the separation chamber 54. A plurality of first filter holes are opened inside the discharge plate 55. Two movable grooves 551 are opened at the top of the discharge plate 55. At one end of the two movable grooves 551 away from their symmetry plane, a plurality of clamping grooves 552 are arranged. The clamping grooves 552 are opened at the top of the discharge plate 55. A gear ring 550 is fixedly connected to the outer surface of the discharge plate 55 to ensure the normal operation of the device.

[0030] The purification device 5 further includes a movable plate 553, which is rotatably connected inside the discharge plate 55. A number of second filter holes corresponding to the size and position of the first filter holes are provided inside the movable plate 553. One end of the movable plate 553 close to the movable groove 551 is fixedly connected with a mounting member 554. The two mounting members 554 extend to the outside of the top of the movable plate 553 through the movable groove 551. Rotating members 555 are rotatably connected to the outer surfaces of the two mounting members 554. A clamping rod adapted to the clamping groove 552 is fixedly connected to the bottom of the rotating member 555. The top part of the stirring rod 53 is rotatably connected inside the discharge plate 55 and the movable plate 553. The bottom of the adjusting disk 52 is rotatably connected to the top of the discharge plate 55 to ensure the normal operation of the device.

[0031] Embodiment 3 Please refer to Figures 1 to 10 , on the basis of Embodiment 2, further, the purification device 5 further includes a second rotation driving component 56, which is fixedly connected to the right side of the inner wall of the top of the purification device 5. The second rotation driving component 56 is set as a driving motor. A second rotating shaft 560 is fixedly connected to the bottom output end of the second rotation driving component 56. A third gear disk 561 is fixedly connected to the outer surface of the second rotating shaft 560 and the third gear disk 561 is meshed with the gear ring 550. A processing chamber 567 is arranged at the bottom of the second rotating shaft 560. The bottom of the processing chamber 567 is fixedly connected to the inner wall of the bottom side of the purification device 5. The second rotating shaft 560 penetrates and extends into the inside of the processing chamber 567 and a third bevel gear 562 is fixedly connected to the extended part of the second rotating shaft 560. A fourth bevel gear 564 is meshed with the left side surface of the third bevel gear 562. A fourth shaft rod 563 is fixedly connected to the right end surface of the fourth bevel gear 564. The other end of the fourth shaft rod 563 is rotatably connected to the right end inner wall of the processing chamber 567. An output shaft 565 is fixedly connected to the left end surface of the fourth bevel gear 564. A fan blade 566 is fixedly connected to the outer surface of the output shaft 565. A heater is fixedly connected to the inside of the processing chamber 567. An air duct is arranged at one end of the processing chamber 567 close to the separation chamber 54 to ensure the normal operation of the device.

[0032] Working principle: Crushing. The black soldier fly protein extraction device is externally connected to a crushing device and a liquid chromatography column. The crushing device crushes a number of black soldier flies to form a black soldier fly protein mixture. At this time, the protein mixture is composed of the black soldier fly protein to be extracted and other proteins. The crushing device can be composed of a speed regulating motor, a reducer, a grinding disk, grinding media, a feeding device, a discharging device, a cooling device and a control system; Feed the black soldier fly protein mixture formed by crushing through a crushing device into a storage device 3, and add an organic solvent into the interior of the storage device 3. At this time, the control center 30 is used to start the first electric turntable 31 to rotate rapidly, so that the organic solvent can be mixed with the black soldier fly protein mixture. After standing still, the black soldier fly protein mixture is stratified. At this time, the black soldier fly protein to be extracted is in the middle of the organic solvent and the remaining proteins; Separation and extraction: Using the control center 30 to activate the adjustment device 4, the adjustment device 4 drives the through rod to move downward. The downward movement of the through rod drives the purification device 5 to move to the inside of the storage bin 32. Initially, the separation grooves 541 at the separation bottom plate 540 are staggered from the separation grooves 541 at the isolation plate 543, the first filter holes and the second filter holes at the separation bottom plate 540 and the movable plate 553 are staggered from each other, the first gear disk 520 is staggered from the two second gear disks 522, the chuck 536 is engaged with the clamping plate 542, and the first bevel gear 530 is staggered from the second bevel gear 531. When the purification device 5 enters the inside of the storage bin 32, it first passes through the organic solvent layer. At this time, since the bottom of the separation bin 54 is a closed space, the organic solvent cannot enter the separation bin 54 through the separation grooves 541 at the separation bottom plate 540 and the isolation plate 543. When the bottom of the separation bottom plate 540 passes through the organic solvent and starts to contact the black soldier fly protein, use the control center 30 to activate the first rotation drive assembly 51. The first rotation drive assembly 51 starts to drive the first rotating shaft 510 to rotate. The rotation of the first rotating shaft 510 drives the second shaft rod 523, the rotating rod 535 and the chuck 536 to rotate through the linear drive assembly 511 and the output rod 512. The rotation of the chuck 536 drives the isolation plate 543 to rotate through the clamping plate 542 until the separation groove 541 at the isolation plate 543 corresponds to the separation groove 541 at the separation bottom plate 540. At this time, use the control center 30 to activate the linear drive assembly 511. The linear drive assembly 511 starts to drive the output rod 512 to move upward. The upward movement of the output rod 512 causes the first gear disk 520 to be meshed with the two second gear disks 522. At the same time, the upward movement of the output rod 512 drives the second shaft rod 523 to move upward. The upward movement of the second shaft rod 523 drives the first bevel gear 530 to be meshed with the second bevel gear 531, and the chuck 536 separates from the clamping plate 542. At this time, the black soldier fly protein can enter the separation bin 54 through the separation grooves 541 at the separation bottom plate 540 and the isolation plate 543. Use the control center 30 to control the first electric turntable 31 to drive the storage bin 32 to rotate slowly. The rotation of the storage bin 32 causes the black soldier fly protein inside the storage bin 32 to continuously enter the separation bin 54 through the separation grooves 541 at the separation bottom plate 540 and the isolation plate 543. At this time, the rotation of the first rotation drive assembly 51 driving the first rotating shaft 510 can drive the first linear drive assembly 511 and the first output rod 512 to rotate. The rotation of the first output rod 512 drives the first gear disk 520 and the two second gear disks 522 to rotate. The rotation of the two second gear disks 522 drives the adjustment disk 52 to rotate through the engaging teeth. The rotation of the adjustment disk 52 drives the stirring rod 53 to rotate. And the rotation of the first output rod 512 can also drive the second shaft rod 523 to rotate. The rotation of the second shaft rod 523 drives the first bevel gear 530 to rotate. The rotation of the first bevel gear 530 drives the second bevel gear 531 to rotate. The rotation of the second bevel gear 531 drives the fixed rod 532 and the stirring plate 533 to rotate.The rotation of the stirring plate 533 drives the adsorption plate 534 to rotate. At this time, according to the rotation of the above-mentioned stirring rod 53, the fixed rod 532, the stirring plate 533, and the adsorption plate 534 can also be driven to rotate in the horizontal direction. The rotation of the stirring rod 53 enables the adsorption plate 534 to bring the black soldier fly protein entering through the separation tank 541 to the inner part of the end of the separation chamber 54 far from the separation tank 541. The rotation of the adsorption plate 534 can further increase the amount of black soldier fly protein that can be transported in one rotation of the adsorption plate 534. Thus, the device can avoid the extraction of organic solvents and black soldier fly protein together when purifying and extracting black soldier fly protein, further improving the purification and extraction efficiency of the device. At the same time, after the extraction is completed, the device can stagger the separation tank 541 at the separation bottom plate 540 and the isolation plate 543 in the same way as above. At this time, a closed space is formed again inside the separation chamber 54, so as to avoid the extraction and mixing of a large amount of other proteins and black soldier fly protein together, thereby greatly reducing the purification and extraction quality of the device, improving the extraction efficiency and purification and extraction quality of the device for black soldier fly protein, and facilitating user use; After the extraction of black soldier fly protein is completed, the control center 30 is used to activate the second electric turntable 41. The second electric turntable 41 drives the purification device 5 to rotate 180 degrees. Then, the control center 30 is used to activate the second rotation drive assembly 56. The activation of the second rotation drive assembly 56 drives the second rotating shaft 560 to rotate. The rotation of the second rotating shaft 560 drives the third gear disk 561 and the third bevel gear 562 to rotate. The rotation of the third gear disk 561 drives the gear ring 550 and the separation bottom plate 540 to rotate. The rotation of the separation bottom plate 540 drives the separation chamber 54 to rotate. At this time, the rotation of the separation chamber 54 can perform centrifugal treatment on the black soldier fly protein inside it, so that the black soldier fly protein and mixtures such as organic solvents are separated again. If the device wants to perform centrifugal treatment on other proteins for secondary separation, it can be carried out without activating the second electric turntable 41. The second electric turntable 41 is used to reset the purification device 5. Then, the user can manually separate the clamping rod from the card slot 552 and rotate the rotating part 555 by opening the opening and closing door 50. The user manually rotates the movable plate 553 through the two mounting parts 554 to overlap the first filter hole and the second filter hole. The mixtures such as organic solvents can be discharged through the first filter hole and the second filter hole. The single black soldier fly protein is stored in the separation chamber 54, avoiding the extraction and mixing of the remaining mixed liquid and black soldier fly protein together, thereby greatly reducing the purification and extraction quality of the device, improving the extraction efficiency and purification and extraction quality of the device for black soldier fly protein, and facilitating user use; In the above step, the rotation of the third bevel gear 562 drives the rotation of the fourth bevel gear 564. The rotation of the fourth bevel gear 564 drives the rotation of the output shaft 565 and the fan blade 566. The number of teeth of the third bevel gear 562 is greater than that of the fourth bevel gear 564, ensuring that the fourth bevel gear 564 can have a sufficient rotation frequency to make the fan blade 566 blow air. The air formed by the rotation of the fan blade 566 blows to the outer surface of the separation chamber 54 under the heating of the heater. By heating the separation chamber 54 during centrifugation, the black soldier fly protein is heated, enabling it to be separated from other mixtures more quickly during centrifugation, improving the extraction efficiency and purification quality of the black soldier fly protein by the device, and facilitating user operation. For secondary separation, the purification device 5 is activated to discharge the remaining partially adhered proteins together to prevent them from reducing the extraction and purification quality of the black soldier fly protein. Subsequently, the extracted black soldier fly protein is transported into a liquid chromatography column for purification, and the purified black soldier fly protein is washed with an eluent to obtain a single black soldier fly protein.

[0033] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for extracting black soldier fly protein, comprising a mounting base plate (1) for supporting a black soldier fly protein extraction device and a device body (2), wherein the device body (2) comprises a storage device (3) for loading a black soldier fly protein mixture, an adjustment device (4) for height adjustment, and a purification device (5) for purifying and extracting black soldier fly protein, characterized in that: Said: Crushing: The black soldier fly protein extraction equipment is connected to a crushing device and a liquid chromatography column. The crushing device crushes a number of black soldier flies to form a black soldier fly protein mixture. At this time, the protein mixture consists of the black soldier fly protein to be extracted and other proteins. The crushing device can be composed of a speed regulating motor, a reducer, a grinding disc, a grinding medium, a feeding device, a discharging device, a cooling device and a control system. Feeding, the black soldier fly protein mixture formed by the pulverizing device is fed into the storage device (3), and the organic solvent is added into the storage device (3) to mix with the black soldier fly protein mixture, so that the black soldier fly protein mixture is layered, and the black soldier fly protein to be extracted is located between the organic solvent and the remaining proteins; Separation and extraction, start the regulating device (4), the regulating device (4) moves the purification device (5) to the storage device (3), and then start the purification device (5) to separate and extract the black soldier fly protein between the organic solvent and the remaining proteins; Secondary separation, the purification device (5) is opened to discharge the remaining partially adhered proteins to avoid reducing the extraction and purification quality of the black soldier fly protein, and then the extracted black soldier fly protein is transported to a liquid chromatography column for purification, and the purified black soldier fly protein is washed with an eluent to obtain a single black soldier fly protein.

2. A black soldier fly protein extraction device, using a black soldier fly protein extraction method as claimed in claim 1, characterized in that: The storage device (3) comprises a control center (30) fixedly connected to the front end surface of the storage device (3); a first electric turntable (31) is fixedly connected to the top of the storage device (3); the first electric turntable (31) is electrically connected to the control center (30); and a storage bin (32) is fixedly connected to the top output end of the first electric turntable (31).

3. The black soldier fly protein extraction device according to claim 1, characterized in that: The regulating device (4) is configured as an electric push rod, and the regulating device (4) comprises a through rod, which is fixedly connected to the top output end of the regulating device (4), and the end of the through rod away from the regulating device (4) is fixedly connected to a connecting rod (40), the top of the connecting rod (40) is fixedly connected to a second electric turntable (41), and the left output end of the second electric turntable (41) is fixedly connected to a purification device (5).

4. A black soldier fly protein extraction device according to claim 3, characterized in that: The purification device (5) comprises an opening and closing door (50) which is rotatably connected to the front end surface of the purification device (5); a first rotation drive component (51) is fixedly connected to the left side of the top inner wall of the purification device (5); a first rotation shaft (510) is fixedly connected to the bottom output end of the first rotation drive component (51); a linear drive component (511) is fixedly connected to one end of the first rotation shaft (510) away from the first rotation drive component (51); the linear drive component (511) is configured as a hydraulic cylinder; and an output rod (512) is differentially connected to the bottom output end of the linear drive component (511).

5. The black soldier fly protein extraction device according to claim 4, characterized in that: The purification device (5) further comprises an adjustment disk (52) which is arranged inside the purification device (5), an output rod (512) passes through the adjustment disk (52) and extends to the outside of the bottom of the adjustment disk (52), a first toothed disk (520) is fixedly connected to a portion of the outer surface of the output rod (512) inside the adjustment disk (52), a first shaft rod (521) is rotatably connected to the left and right sides of the inner wall of the bottom side of the adjustment disk (52), a second toothed disk (522) is fixedly connected to the center of the outer surface of the first shaft rod (521), a plurality of latching teeth are fixedly connected to the inner wall of the adjustment disk (52) close to the second toothed disk (522), and the second toothed disk (522) is meshedly connected to the inside of the adjustment disk (52) through the latching teeth.

6. The black soldier fly protein extraction device according to claim 5, characterized in that: The purification device (5) further comprises a stirring rod (53) which is fixedly connected to the bottom of the regulating disk (52); an extension portion of the first shaft rod (521) penetrates into the interior of the stirring rod (53); a second shaft rod (523) is fixedly connected to the bottom of the first shaft rod (521) inside the stirring rod (53); a first bevel gear (530) is fixedly connected to the bottom of the second shaft rod (523); a second bevel gear (531) is meshedly connected to the right side of the first bevel gear (530); an end of the second bevel gear (531) away from the first bevel gear (530) is fixedly connected to the first bevel gear (530); A fixed rod (532) is connected, the fixed rod (532) passes through the stirring rod (53) and extends to the outside of the right end of the stirring rod (53), a stirring plate (533) is fixedly connected to the extended portion of the fixed rod (532), the top and bottom of the stirring plate (533) are fixedly connected to an adsorption plate (534), the bottom of the first bevel gear (530) is fixedly connected to a rotating rod (535), the rotating rod (535) passes through the stirring rod (53) and extends to the outside of the bottom of the stirring rod (53), and a clamp (536) is fixedly connected to the extended portion of the rotating rod (535).

7. The black soldier fly protein extraction device according to claim 6, characterized in that: The purification device (5) further comprises a separation chamber (54), which is arranged on the outer surface of the stirring rod (53); a separation bottom plate (540) is fixedly connected to the bottom of the separation chamber (54); an isolation plate (543) is arranged inside the separation bottom plate (540); a clamping plate (542) adapted to the clamping head (536) is fixedly connected to the top of the isolation plate (543); the clamping plate (542) penetrates the separation bottom plate (540) and extends to the outer side of the top of the separation bottom plate (540); a third shaft rod (544) is fixedly connected to the bottom of the isolation plate (543); the third shaft rod (544) is rotatably connected to the inner wall of the bottom side of the separation bottom plate (540); and separation grooves (541) are provided at the top and bottom of the separation bottom plate (540) and inside the isolation plate (543).

8. The black soldier fly protein extraction device according to claim 7, characterized in that: The purification device (5) further comprises a discharge plate (55) which is fixedly connected to the top of the separation chamber (54); a plurality of first filter holes are provided inside the discharge plate (55); two movable grooves (551) are provided on the top of the discharge plate (55); a plurality of clamping grooves (552) are provided at one end of the two movable grooves (551) away from the symmetry plane thereof; the clamping grooves (552) are provided at the top of the discharge plate (55); and a gear ring (550) is fixedly connected to the outer surface of the discharge plate (55).

9. The black soldier fly protein extraction device according to claim 6, characterized in that: The purification device (5) further comprises a movable plate (553) which is rotatably connected to the inside of the discharge plate (55); a plurality of second filter holes corresponding to the size and position of the first filter holes are provided inside the movable plate (553); a mounting member (554) is fixedly connected to one end of the movable plate (553) close to the movable groove (551); two mounting members (554) extend through the movable groove (551) to the outside of the top of the movable plate (553); rotating members (555) are rotatably connected to the outer surfaces of the two mounting members (554); a clamping rod matching the clamping groove (552) is fixedly connected to the bottom of the rotating member (555); the top portion of the stirring rod (53) is rotatably connected to the inside of the discharge plate (55) and the movable plate (553); and the bottom of the regulating disk (52) is rotatably connected to the top of the discharge plate (55).

10. The black soldier fly protein extraction device according to claim 9, characterized in that: The purification device (5) further comprises a second rotary drive assembly (56), which is fixedly connected to the right side of the top inner wall of the purification device (5), the second rotary drive assembly (56) being configured as a drive motor, a second rotating shaft (560) being fixedly connected to the bottom output end of the second rotary drive assembly (56), a third toothed disc (561) being fixedly connected to the outer surface of the second rotating shaft (560), and the third toothed disc (561) being meshingly connected to the gear ring (550), a processing chamber (567) being disposed at the bottom of the second rotating shaft (560), the bottom of the processing chamber (567) being fixedly connected to the bottom inner wall of the purification device (5), and the second rotating shaft (560) passing through and extending to the processing chamber (567). A third bevel gear (562) is fixedly connected to the interior of the processing chamber (567) and the extension of the second rotating shaft (560); a fourth bevel gear (564) is meshingly connected to the left side surface of the third bevel gear (562); a fourth shaft rod (563) is fixedly connected to the right end surface of the fourth bevel gear (564); the other end of the fourth shaft rod (563) is rotatably connected to the right end inner wall of the processing chamber (567); an output shaft (565) is fixedly connected to the left end surface of the fourth bevel gear (564); a fan blade (566) is fixedly connected to the outer surface of the output shaft (565); a heater is fixedly connected to the interior of the processing chamber (567); and an air duct is provided at one end of the processing chamber (567) close to the separation chamber (54).

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