Cutting propagation method for dalbergia odorifera

Abstract

The invention provides a cutting propagation method for dalbergia odorifera. The cutting propagation method comprises a cutting method and a propagation method using the cutting method. The cuttage method comprises the following steps: (1) treatment of cutting slips; (2) soaking: soaking the treated dalbergia odorifera cutting slips in an IBA solution with the concentration of 450-550 mg / L for 25-35 minutes, taking out the dalbergia odorifera cutting slips, and washing lower cuts with water; (3) cutting: cutting the soaked dalbergia odorifera cuttings into a substrate which comprises river sand and red soil in a mass ratio of (2.5-3.5): 1; (4) covering the seedling raising bag after cuttage with a thin film, covering the thin film with a layer of sunshade net, and watering to keep the substrate in the seedling raising bag moist. By using the IBA solution with proper concentration, specific soaking time and specific cutting medium, the cutting survival rate of the dalbergia odorifera can be effectively increased. Further, effective management and control of illumination, humidity and temperature series conditions are combined, so that the dalbergia odorifera cutting seedlings for planting can be efficiently obtained, and the cutting propagation of the dalbergia odorifera is effectively realized.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of plant propagation, in particular to cutting propagation of Dalbergia tsoi, and specifically relates to a cutting propagation method of Dalbergia tsoi. BACKGROUND

[0002] Dalbergia tsoi, also known as Dalbergia tsoi, Baisha Dalbergia, Baisha Dalbergia, and red fruit Dalbergia, is a liana of Dalbergia in Leguminosae, and only distributes in Hainan, China, and is a wild plant unique to Hainan. Dalbergia tsoi is a woody liana for producing Xiangjiang, and is a Xiangjiang base plant in Hainan. The Xiangjiang body is commonly known as "ten mu Xiang" in the market. Ten mu Xiang is a rare product of Xiangjiang, a precious medicinal material and incense material, and also a precious material for handicrafts, and its use is very wide. According to the physical appreciation, ten mu Xiang itself has rich and lasting fragrance, and smells like honey, flowers, medicine, coconut and cool sweet. Hainan folk has regarded it as a rare product since ancient times, especially in minority areas. Field investigation proves that it is believed to have the effects of activating blood and resolving stasis, expelling wind and dampness, relieving swelling and bleeding, and detoxifying, and is often used as an external medicine for bruises, cuts, burns, insect and snake bites, and also can be taken orally as a medicine for prevention and treatment of cardiovascular diseases. In addition, the vine material of Dalbergia tsoi processed into root carving, ornaments, prayer beads, bracelets and necklaces after Xiangjiang has high economic value and collection value.

[0003] Investigation found that the number is small and the distribution is scattered, the natural fruiting effect is not good, and most of the plants do not bear fruit, and the natural propagation effect is poor. Developing artificial cultivation of Dalbergia tsoi, seedling is the key, because Dalbergia tsoi resources are few and seriously damaged, seed collection mother tree (mother vine) is scarce, seed collection is difficult, and seedling cultivation is not easy. Therefore, solving the problem of seedling propagation of Dalbergia tsoi can realize the protection of wild resources of Dalbergia tsoi, and can support the large-scale production through technical support, support the follow-up development of Xiangjiang industry, and has important significance for maintaining and improving forest ecological efficiency and promoting sustainable development of industry. SUMMARY

[0004] Therefore, the purpose of the present application is to provide a cutting propagation method of Dalbergia tsoi, which can effectively solve the problem of seedling propagation of Dalbergia tsoi.

[0005] In order to achieve the above purpose, the following technical scheme is adopted.

[0006] The first aspect of the present application provides a cutting method of Dalbergia tsoi, comprising the following steps:

[0007] (1) cutting treatment: taking Dalbergia tsoi cutting, removing leaves, cutting the upper end flat, cutting the lower end obliquely, disinfecting, and washing with water;

[0008] (2) soaking: soaking the treated Dalbergia henryana cutting in 450mg / L-550mg / L indole butyric acid (IBA) solution for 25min-35min, taking out, washing the incision with water;

[0009] (3) cutting: cutting the soaked Dalbergia henryana cutting into the substrate of the seedling bag, and pouring water thoroughly; the substrate comprises river sand and red soil, and the mass ratio of the two is (2.5-3.5):1;

[0010] (4) covering the cutting seedling bag with a film, covering 1 layer of sunshade net outside the film, and pouring water to keep the substrate in the seedling bag moist.

[0011] In some embodiments, the treated Dalbergia henryana cutting is soaked in 480mg / L-520mg / L indole butyric acid solution in step (2).

[0012] In some embodiments, the substrate comprises river sand and red soil, and the mass ratio of the two is (3-3.5):1 in step (3).

[0013] In some embodiments, the cutting is performed in February-March.

[0014] In some embodiments, the Dalbergia henryana cutting is a 1-2 year old main branch.

[0015] In some embodiments, the main branch is a main branch tip.

[0016] In some embodiments, the sunshade net has a light blocking rate of 70%-75% in step (4).

[0017] The second aspect of the application provides the use of the cutting method as described above in the propagation of Dalbergia henryana.

[0018] The third aspect of the application provides a propagation method of Dalbergia henryana, comprising the following steps:

[0019] (1) using the cutting method as described above to cut;

[0020] (2) selecting and reserving 1 sprout when the root system grows out and the sprout length is 5cm-10cm; removing the film and the sunshade net, and placing the seedling bag in a nursery with sunshade net;

[0021] (3) transplanting when the cutting seedling height is ≥30cm.

[0022] In some embodiments, the transplanting method is as follows: applying organic fertilizer and manure in the planting hole, and mixing with the original soil in the hole; planting the cutting seedling, tamping the backfilling soil, pouring sufficient water for root fixation, and spreading a layer of floating soil again; and watering, weeding and protecting after planting.

[0023] The present application optimizes a cutting method of Dalbergia hupeana through a large amount of research, and the cutting survival rate of Dalbergia hupeana can be effectively improved (up to 87.30±7.27%) after soaking in a suitable concentration of indole butyric acid and then cutting into a specific substrate (a substrate composed of river sand and red soil in a mass ratio of 2.5-3.5:1) for a short soaking time of about 30 min. Further combined with the control of effective light, humidity and temperature series conditions, the cutting seedlings for planting can be efficiently obtained, and the propagation of Dalbergia hupeana is successfully realized.

[0024] The present application effectively solves the seedling propagation problem of Dalbergia hupeana, and has important significance for the protection of wild resources of Dalbergia hupeana, the development of Dalbergia odorifera industry, the maintenance and improvement of forest ecological efficiency, and the promotion of sustainable development of industry. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 Part of the under-forest planting demonstration map.

[0026] Figure 2 Cutting survival rate of Dalbergia hupeana under IBA treatment.

[0027] Figure 3 Cutting survival rate of Dalbergia hupeana under different substrate treatments.

[0028] Figure 4 Cutting survival rate of Dalbergia hupeana shoot under different water management.

[0029] Figure 5 Cutting survival rate of Dalbergia hupeana shoot under different light management.

[0030] Figure 6 Cutting survival rate of Dalbergia hupeana shoot under different temperature.

[0031] Figure 7 Cutting survival rate of Dalbergia hupeana shoot under different humidity. DETAILED DESCRIPTION

[0032] The experimental methods not specified in the following examples of the present application are usually carried out according to the conventional conditions, or according to the conditions recommended by the manufacturers. Various common chemical reagents used in the examples are commercially available products.

[0033] Unless otherwise defined, all technical and scientific terms used in the present application have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terms used in the specification of the present application are only for the purpose of describing specific embodiments and are not used to limit the present application.

[0034] The terms "comprising" and "having" and any variations thereof herein are intended to cover a non-exclusive inclusion. For example, a process, method, article, or apparatus that comprises a list of steps or modules is not necessarily limited to those listed steps or modules, but can include additional steps or modules not expressly listed or additional steps or modules that are inherent to such process, method, article, or apparatus.

[0035] In the present application, "and / or" is used to describe the relationship between the associated objects, which means that there can be three relationships, for example, A and / or B, which can represent three cases: A exists alone, A and B exist together, and B exists alone.

[0036] The following is described in conjunction with specific embodiments.

[0037] The materials used in the following examples are as follows: The test branches are red fruit Dalbergia branches collected in Liumushan National Forest Park in Qiongzhong Li and Miao Autonomous County, Hainan Province. In February and March, the main stem part of Dalbergia odorifera plants is selected, and semi-lignified to lignified branches are used as cutting materials. The test is carried out in the cutting propagation experiment shed of the forest farm.

[0038] Observation and statistics: After cutting, observe the germination and survival of the cuttings, and determine the survival standard as rooting and germination of the cuttings. Data statistics is carried out at 45d, and the survival rate = the number of surviving cuttings / total number of cuttings x 100%.

[0039] Example 1

[0040] The present embodiment provides a cutting propagation method of Dalbergia odorifera.

[0041] The cutting substrate used in the present embodiment is prepared by the following method: 7*14cm degradable seedling bags are selected, and sieved river sand and red soil are mixed at a ratio of 3:1 to prepare the cutting substrate. After the mixed substrate is bagged, it is poured with a carbendazim solution for disinfection 3-5 days before cutting to prevent bacterial and viral infection of the cutting incision.

[0042] The preparation method of the IBA solution used in the present embodiment is as follows: 0.5g IBA powder is completely dissolved with a small amount of alcohol, and then purified water is added to 1L to prepare an IBA solution with a concentration of 500mg / L, which is stirred and ready for use.

[0043] The cutting propagation method comprises the following steps:

[0044] (1) Cutting treatment

[0045] Cut the branches into 10-15 cm long cuttings, remove the leaves, cut the upper end flat and the lower end obliquely; ensure that there are at least two healthy bud points on the cutting. Bundle the pruned cuttings according to a certain number with rubber bands, soak them in a carbendazim solution for 10 minutes, then take them out and rinse them clean with clean water, and prepare to soak the prepared rooting agent.

[0046] (2) Soaking

[0047] Align the lower part of the Dalbergia odorifera cutting, immerse the cut end in the IBA solution prepared in advance with a concentration of 500 mg / L, so that the solution is about 2 cm higher than the base of the cutting, soak for 30 min, take it out immediately, and rinse the lower cut with clean water.

[0048] (3) Cutting

[0049] Use the straight insertion method for cutting, with a cutting depth of about 3.5 cm, insert the cuttings into the prepared cutting bags, and pour water thoroughly after cutting.

[0050] (4) Daily care

[0051] Arrange the cutting seedling bags neatly after cutting, and cover them with bamboo splints and thin film, and then cover them with another layer of shading net with a shading rate of 75% to prevent direct sunlight.

[0052] After cutting, keep the soil in the cutting bag moist, but do not accumulate water, do not pour too much water each time, and keep it moist; use carbendazim for disinfection once every 7 days.

[0053] Germination will begin about 7-10 days after cutting, at which time new roots have not yet grown, so the new shoots that grow too fast should be pruned appropriately, and when the root system grows out after about 40 days, select and keep one of the 5-10 cm sprouts. At this time, remove the film and shading net for hardening-off, and place the seedling bags in the nursery with shading net. According to statistics, the survival rate of cutting is as high as 87.30±7.27%.

[0054] (5) Out-of-nursery planting

[0055] When the seedlings are 30 cm tall, they can be planted out.

[0056] Site: Choose places such as open forest, forest edge, valley, etc. as planting plots.

[0057] Soil preparation: Clear the surface grass and shrubs before planting to facilitate planting and management, and prevent weeds and shrubs from affecting seedling growth.

[0058] Seedlings and specifications: Dalbergia odorifera cutting seedlings, seedling height ≥ 30 cm.

[0059] Planting hole specifications: 30 cm x 30 cm x 30 cm.

[0060] Row spacing: 1.5 m x 2 m.

[0061] Base fertilizer: Apply organic fertilizer and manure in the planting hole and mix with the original soil in the hole.

[0062] Planting: Keep the seedling straight during planting, and tamp the backfilling soil; then pour enough water for root fixation, and spread a layer of floating soil.

[0063] Maintenance: Water once again after 5-7 days of planting; weed in time after planting, water during drought, and replant after 1 month. After the seedling grows normally, only simple weeding is needed.

[0064] Using the collected Dalbergia hancei germplasm resources and the cuttings of Dalbergia hancei seedlings, a 2.1 mu Dalbergia hancei resource nursery and a 5.3 mu under-forest planting demonstration site were established in the Maple Experimental Forest Farm in Hainan Province, and part of the results are shown in Table 1. Figure 1

[0065] Example 2

[0066] This example studies the effect of growth regulators on the survival rate of Dalbergia hancei cuttings.

[0067] I. Experimental method

[0068] Five concentrations were set: 0 mg / L, 250 mg / L, 500 mg / L, 750 mg / L, and 1000 mg / L, and four soaking times were set: 30 s, 5 min, 30 min, and 120 min. Each treatment had 21 cuttings, with three replicates. The experimental design is shown in Table 1:

[0069] Table 1 Experimental design

[0070]

[0071] Cutting substrate: To facilitate root observation and data collection, the substrate was selected as soilless cutting sponge. The sponge size was 20 x 30 x 3.5 cm, and two layers of sponge were stacked together and placed in a seedling tray. The sponge was soaked in a 1000-fold solution of carbendazim for 3 days in advance for disinfection.

[0072] The cutting method is as follows:

[0073] Soaking with growth regulators and cutting: Align the lower part of the cutting, and immerse the cut end in the prepared growth regulator solution, with the solution about 2 cm higher than the cutting base. Immediately take out and rinse clean after the soaking time. Use the straight insertion method for cutting, with a cutting depth of about 3.5 cm, so that the cutting base is between the two layers of sponge. Immediately pour water completely after cutting.

[0074] Daily management: Water once a day after cutting, and disinfect with carbendazim once every 7 days. ​

[0075] Observation and statistics: After cutting, pay attention to the germination and survival of the cuttings. The standard for judging survival is that the cuttings take root and germinate. Data statistics are performed after 45 days. Survival rate = number of surviving cuttings / total number of plants × 100%.

[0076] 2. Experimental Results

[0077] Depend on Figure 2 It can be seen that under the same IBA concentration and different soaking time treatments, the survival rate of Dalbergia rubra cuttings is significantly different; with the increase of soaking time, the change trend of the survival rate at concentrations of 250 mg / L, 500 mg / L and 750 mg / L is basically the same, all of which first increase and then decrease; at the concentration of 1000 mg / L, it shows a downward trend.

[0078] The survival rate of Dalbergia rubrotinensis cuttings at 250 mg / L, 500 mg / L, and 750 mg / L IBA concentrations showed a trend of increasing first and then decreasing with increasing immersion time. At a 250 mg / L IBA concentration, the highest survival rate (46.03 ± 7.27%) was achieved after a 30-minute immersion, which was 1.93 times the average immersion time (23.84%) of the CK. The survival rate of Dalbergia rubrotinensis cuttings ranked in the order of 30 min > 5 min > 120 min > 30 s. At a 500 mg / L IBA concentration, the highest survival rate (69.84 ± 11.98%) was achieved after a 30-minute immersion, which was 2.93 times the average immersion time (23.84%) of the CK. The survival rate of Dalbergia rubrotinensis cuttings ranked in the order of 30 min > 5 min > 120 min > 30 s. At an IBA concentration of 750 mg / L, the 5-minute immersion treatment achieved the highest survival rate (44.44 ± 9.91%), 1.86 times the average CK rate (23.84%). The survival rate of Dalbergia rubra cuttings decreased in the order of 5 min > 30 min > 30 s > 120 min. At an IBA concentration of 1000 mg / L, the survival rate of all treatments was lower than that of the CK group.

[0079] Under the same soaking time and different IBA concentrations, the survival rate of Dalbergia rubra cuttings was significantly different; with the increase of the concentration of the agent, the change trend of the survival rate under different soaking time treatments was basically the same, that is, it first increased and then decreased.

[0080] The survival rate was the highest (42.86±4.76%) at a concentration of 500 mg / L under the treatment of 30 s soaking time, and the survival rate was 1.80 times of the average value (23.84%) of CK; the survival rate of Dalbergia hancei was 500 mg / L>750 mg / L>250 mg / L>1000 mg / L in turn. The survival rate was the highest (55.56±16.72%) at a concentration of 500 mg / L under the treatment of 5 min soaking time, and the survival rate was 2.33 times of the average value (23.84%) of CK; the survival rate of Dalbergia hancei was 500 mg / L>750 mg / L>250 mg / L>1000 mg / L in turn. The survival rate was the highest (69.84±11.98%) at a concentration of 500 mg / L under the treatment of 30 min soaking time, and the survival rate was 2.93 times of the average value (23.84%) of CK; the survival rate of Dalbergia hancei was 500 mg / L>250 mg / L>750 mg / L>1000 mg / L in turn. The survival rate was the highest (46.03±9.91%) at a concentration of 500 mg / L under the treatment of 120 min soaking time, and the survival rate was 1.93 times of the average value (23.84%) of CK; the survival rate of Dalbergia hancei was 500 mg / L>250 mg / L>750 mg / L>1000 mg / L in turn.

[0081] According to the above results, the survival rate was the highest (69.84±11.98%) at a concentration of 500 mg / L under the treatment of 30 min soaking time, and the coefficient of variation was 17.15%.

[0082] In this embodiment, various growth regulators were also studied, and finally it was found that indole butyric acid IBA was most beneficial to the survival rate of Dalbergia hancei cutting. Naphthalene acetic acid NAA, indole acetic acid IAA and ABT were taken as examples for comparison. Each group was different in the growth regulator used for soaking, and the others were the same. Each treatment had 21 cuttings, and 3 repetitions.

[0083] The results are shown in Table 2.

[0084] Table 2

[0085]

[0086] As shown in Table 2, 500 mg / L IBA soaking time 30 min can obtain better cutting survival rate.

[0087] Embodiment 3

[0088] In this embodiment, the effect of substrate on the survival rate of Dalbergia hancei cutting was studied.

[0089] The present embodiment studies various cutting substrates, taking soilless cultivation sponge, river sand, red soil, perlite, and river sand and red soil mixed at a ratio of 3:1 as examples for comparison and illustration. According to the research results of Example 2, the treatment of soaking in 500 mg / L IBA solution for 30 min is selected.

[0090] I. Materials and Methods

[0091] Materials: The test branches were collected from the red fruit Dalbergia henryana in the Liumushan National Forest Park in Qiongzhong Li and Miao Autonomous County, Hainan Province. The main stem of D. henryana was selected, and semi-lignified to lignified branches were used as cutting materials. The test was conducted in the cutting propagation experiment shed of the forest farm.

[0092] Reagents: Indolebutyric acid (IBA) was prepared into a 500 mg / L solution.

[0093] Cutting treatment: The selected D. henryana stem was cut into 10-15 cm long cuttings, the upper end was cut flat, and the lower end was cut obliquely; at least two healthy bud points were ensured on the cutting.

[0094] Test design: Four substrates (soilless cultivation sponge, river sand, red soil, perlite, and river sand and red soil mixed at a ratio of 3:1) were set up, a total of 5 treatments, 21 cuttings per treatment, 3 replicates (Table 3).

[0095] Table 3 Design of cutting experiment with different substrates

[0096]

[0097] Substrate preparation: Each substrate was placed in a seedling tray and soaked in a 1000-fold solution of carbendazim for 3 days in advance for disinfection. River sand, red soil, and mixed substrates were first screened with a soil sieve to remove large particles, and then used as substrates.

[0098] Soaking with chemicals and cutting: The lower part of the test cutting was aligned, the cut was soaked in the prepared 500 mg / L IBA solution, the solution was about 2 cm higher than the base of the cutting, and the soaking time was immediately taken out and washed clean. The straight insertion method was used for cutting, the cutting depth was about 3.5 cm, and the cutting was immediately watered completely.

[0099] Daily management: After cutting, water was added in time according to the moisture content of the substrate, but river sand and red soil and mixed substrates should not be watered too much to prevent cutting from rotting; carbendazim was used for disinfection once every 7 days.

[0100] Observation and statistics: After cutting, the germination and survival of the cuttings were observed, the survival standard was that the cutting rooted and germinated, and the data was counted at 45 d. Survival rate = number of surviving cuttings / total number of cuttings × 100%

[0101] II. Results and Analysis

[0102] Results show (Table 4 and Figure 3 ), with 500mg / L IBA solution as cutting agent, the survival rate of cutting in the substrate of mixed river sand and red soil at 3:1 is the highest, reaching 76.19±8.25%; the survival rate of cutting in pure river sand is the lowest, being 47.62±12.60%. The survival rate of cutting in different substrates is in the order of river sand mixed with red soil (76.19±8.25%) > sponge (65.08±7.27%) > perlite (61.90±12.60%) > red soil (52.38±9.52%) > river sand (47.62±12.60%). It is shown that the substrate of mixed river sand and red soil at 3:1 is the most suitable for cutting propagation of Dalbergia hupeana Hance.

[0103] Table 4 Coefficient of variation of survival rate of cutting of Dalbergia hupeana Hance in different substrates

[0104]

[0105] Example 4

[0106] In this example, the effect of cutting environment on the survival rate of cutting is studied.

[0107] The one-year-old main branch tips of Dalbergia hupeana Hance which have not been completely lignified are used as cutting materials. The cuttings are treated with 500mg / L IBA for 30min, and the cutting substrate is mixed river sand and red soil at a weight ratio of 3:1. Under the premise of being close to the actual production and as simple as possible, cutting tests are carried out under different water management, different light, different temperature and different humidity conditions to further improve the survival rate of cutting propagation of Dalbergia hupeana Hance.

[0108] I. Materials and methods

[0109] Cutting materials: The one-year-old main branch tips of Dalbergia hupeana Hance which have not been completely lignified are used as cutting materials.

[0110] Material source: The branches are collected from the Dalbergia hupeana Hance plants which are successfully propagated and cultivated in the preliminary test in Maple Experimental Forest Station, Hainan Province.

[0111] Reagent: Indole-3-butyric acid (IBA) is configured into a 500mg / L solution.

[0112] Cutting substrate: mixed substrate of river sand: red soil = 3:1.

[0113] Cutting treatment: The selected Dalbergia hupeana Hance branches are cut into 10cm-15cm long cuttings, the upper end is cut flat, and the lower end is cut obliquely; at least two healthy bud points are ensured on the cutting.

[0114] Test design:

[0115] A single-factor experimental design was used to set different environmental conditions such as water management, temperature, humidity, and light, with each treatment repeated three times.

[0116] Water management: There are four treatments: watering once a day, watering once every two days, watering once every three days, and flexible watering according to the moisture conditions of the substrate (depending on the moisture conditions of the cutting substrate, keep the substrate moist) (Table 10).

[0117] Different light exposure: Under the premise of full film coverage, different degrees of shading treatment were carried out, including no shading, one layer of 75% shading net, and one layer of 95% shading net (Table 5).

[0118] Table 5 Cutting experiment design with different water and light management

[0119]

[0120]

[0121] Different temperatures: To facilitate production operations and stay close to actual production, we selected natural temperatures from January to May for cuttings, covered with plastic film to maintain consistent humidity. There were five treatments in total, and the temperature settings are shown in Table 6.

[0122] Table 6 Temperature overview from January to May 2023

[0123]

[0124] Different humidity: When taking cuttings in February, the humidity can be simply controlled by using methods such as full coverage with film, half coverage, shade net coverage, and no coverage. The humidity settings are shown in Table 7.

[0125] Table 7 Cutting experiment settings at different humidity levels

[0126]

[0127] 2. Results and Analysis

[0128] (1) Effects of different water management on the survival rate of Dalbergia rubra cuttings

[0129] Depend on Figure 4 The results showed that the survival rate of Dalbergia rubrotinensis cuttings gradually increased with decreasing watering frequency. Watering according to substrate moisture content significantly increased the survival rate, reaching a maximum of 76.19±9.52%. The order of survival rate of Dalbergia rubrotinensis cuttings under different water management conditions was: flexible watering (76.19±9.52%) > once every three days (49.21±9.91%) > once every two days (46.03±9.91%) > once daily (38.10±12.60%).

[0130] The survival rate of Dalbergia rubra cuttings is highest when watering is done flexibly, depending on the moisture level of the substrate. This is likely because flexible watering ensures both moisture and air permeability in the substrate, preventing water accumulation. A mixture of river sand and red soil has a certain water-retention capacity, and excessive watering can easily lead to root rot and necrosis in Dalbergia rubra cuttings, which may be the main reason for the low survival rate.

[0131] As shown in Table 8, the survival rate of Dalbergia rubrotinica cuttings was low and the coefficient of variation was large when watering was performed once a day, once every two days, and once every three days. Flexible watering had the smallest coefficient of variation and the highest survival rate, indicating that flexible watering is the most suitable water management method for Dalbergia rubrotinica cuttings.

[0132] Table 8 Coefficient of variation of survival rate of Dalbergia rubra cuttings under different water management

[0133]

[0134]

[0135] (2) Effects of different light management on the survival rate of Dalbergia rubra cuttings

[0136] Depend on Figure 5 The survival rates of Dalbergia rubra cuttings varied significantly under different light treatments. The highest survival rate (82.54±5.50%) was achieved when covered with 75% shade netting, while the lowest survival rate (66.67±12.60%) was achieved when covered with 95% shade netting. The order of survival rates under different light treatments was: 75% shade netting (82.54±5.50%) > no shade (66.67±12.60%) > 95% shade netting (66.67±12.60%).

[0137] The survival rate of Dalbergia ruber cuttings covered with 95% shade net was the lowest, which may be because the shading was too dense, affecting the photosynthesis of the new shoots of the cuttings, resulting in insufficient nutrients for the cuttings to grow and die; the survival rate of Dalbergia ruber cuttings without shading treatment was slightly lower, which may be because direct sunlight caused some tender cuttings to evaporate water too quickly, resulting in dehydration and death.

[0138] It can be seen from Table 9 that under different light management methods, the higher the survival rate, the lower the coefficient of variation, indicating that under 75% shade net coverage, the environmental conditions are the most stable and most suitable for Dalbergia rubra cuttings.

[0139] Table 9 Coefficient of variation of survival rate of Dalbergia rubra cuttings under different light management

[0140]

[0141] (3) Effects of different temperatures on the survival rate of Dalbergia rubra cuttings

[0142] Depend on Figure 6 It can be seen that under the influence of temperature from January to May, the survival rate of Dalbergia rubra cuttings first increased and then decreased. The survival rate of cuttings in February (87.30±7.27%) was significantly higher than that in January (65.08±5.50%), April (65.08±15.31%), and May (61.90±12.60%). The order of survival rate from January to May was February (87.30±7.27%) > March (76.19±8.25%) = January (65.08±5.50%) > April (65.08±15.31%) > May (61.90±12.60%).

[0143] Table 6 shows that the average low temperature in February was 17°C, similar to the average low temperature of 18°C ​​in March, significantly higher than January but lower than April and May. The average high temperature in February was 25°C, significantly higher than January but lower than March, April, and May. In terms of extreme low temperatures, February and March were similar, while in terms of extreme high temperatures, February, March, and April were similar. Therefore, it is inferred that average high temperatures played a major role in the survival rate of Dalbergia rubra cuttings.

[0144] As shown in Table 10, the survival rate of Dalbergia rubra cuttings was the highest in February, and the coefficient of variation was the smallest (8.33%), indicating that the temperature environment was most suitable under the natural temperature in February, and the survival rate of Dalbergia rubra cuttings was the most stable.

[0145] Table 10 Coefficient of variation of survival rate of Dalbergia rubra cuttings at different temperatures

[0146]

[0147] (4) Effects of different humidity on the survival rate of Dalbergia rubra cuttings

[0148] Depend on Figure 7 It can be seen that with the increase of the average humidity of the cutting environment, the survival rate of Dalbergia rubra gradually increased. The order of the survival rate of Dalbergia rubra under different average humidity conditions is W4 (87.30±7.27%) > W3 (85.71±8.25%) > W2 (65.08±7.27%) > W1 (38.10±8.25%).

[0149] As shown in Table 7, the average humidity of the cutting environment was lower under both the open-air treatment and the shade net cover, and was closer to the natural humidity, with large fluctuations and instability. The humidity was higher and more stable under the semi- and full-film cover.

[0150] From Table 11, it can be seen that the variation coefficient of cutting survival rate of Dalbergia hupeana under open air and sunshade net covering treatment is obviously greater than that under film semi-covering and film full-covering treatment. It is indicated that with the stable humidity of cutting environment, the cutting survival rate is also more stable. Therefore, it can be inferred that higher and stable environmental humidity is more conducive to cutting propagation of Dalbergia hupeana.

[0151] Table 11 Variation coefficient of cutting survival rate of Dalbergia hupeana under different humidity

[0152]

[0153] In summary, flexible watering of Dalbergia hupeana cutting can ensure both substrate moisture and substrate air permeability, and the survival rate is 76.19±9.52%. Too much watering is easy to cause root rot and necrosis of Dalbergia hupeana cutting; 75% sunshade net covering can both let the new shoots of Dalbergia hupeana cutting grow and not cause the cutting to die of dehydration, and also can maintain a certain air humidity, and the survival rate is 82.54±5.50%; the temperature and humidity in February are both relatively suitable for Dalbergia hupeana cutting, and the survival rate is 87.30±7.27%; high humidity is conducive to the survival of Dalbergia hupeana cutting, and the survival rate of Dalbergia hupeana cutting under film semi-covering and full-covering is higher, and the highest is 87.30±7.27%.

[0154] The technical features of the above-described embodiments can be combined in any manner. To make the description concise, all possible combinations of the technical features in the above embodiments are not described, however, as long as the combinations of the technical features do not exist contradictions, they should be considered as the scope of the present disclosure.

[0155] The above-described embodiments only express several implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as a limitation on the patent scope of the present application. It should be noted that, for those skilled in the art, without departing from the concept of the present application, some modifications and improvements can be made, which are all within the protection scope of the present application. Therefore, the patent protection scope of the present application should be subject to the appended claims.

Claims

1. A cutting method for Dalbergia rubra, characterized in that: The following steps are involved: (1) Cutting treatment: Take Dalbergia rubra cuttings, remove the leaves, cut the upper end flat, cut the lower end obliquely, disinfect, and rinse with water; (2) Soaking: Soak the treated Dalbergia rubra cuttings in 450 mg / L to 550 mg / L indolebutyric acid solution for 25 to 35 minutes, take them out, and rinse the cut surface with water; (3) Cutting: The soaked Dalbergia rubra cuttings are inserted into the matrix of the seedling bag and watered thoroughly; the matrix comprises river sand and red soil, and the mass ratio of the two is (2.5-3.5):1; (4) Cover the seedling bag after cutting with a film, cover the film with a layer of sunshade net, and water to keep the substrate in the seedling bag moist.

2. The cutting method according to claim 1, wherein In step (2), the treated Dalbergia rubra cuttings are immersed in a 480 mg / L to 520 mg / L indolebutyric acid solution.

3. The cutting method according to claim 1, wherein The matrix in step (3) includes river sand and red soil, and the mass ratio of the two is (3.0-3.5):

1.

4. The cutting method according to claim 1, wherein Cuttings are taken in February and March.

5. The cutting method according to claim 1, wherein The Dalbergia rubra cuttings are 1-2 year old main branches.

6. The cutting method according to claim 5, wherein: The main branches are main branch tips.

7. The cutting method according to claim 1, wherein The shading rate of the sunshade net in step (4) is 70% to 75%.

8. Use of the cutting method according to any one of claims 1 to 7 in the propagation of Dalbergia rubra.

9. A method for propagating Dalbergia rubra, characterized in that: The following steps are involved: (1) cutting by the cutting method according to any one of claims 1 to 7; (2) When the root system grows out and the length of the sprout is 5 cm to 10 cm, select one sprout and keep it; remove the film and shade net, and place the seedling bag in the nursery with the shade net; (3) When the cutting seedlings are ≥30cm tall, they can be transplanted into the field.

10. The propagation method according to claim 9, wherein The method for transplanting is as follows: applying organic fertilizer and manure in the planting hole and mixing them with the original soil in the hole; planting the cutting seedlings, compacting the backfill soil, watering them enough for rooting, and spreading a layer of floating soil; and watering, weeding and maintaining the plants after planting.

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