Composite peptide-containing composition for improving skin elasticity and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, in particular to a composite peptide-containing composition for increasing skin elasticity and a preparation method thereof, and the composite peptide-containing composition comprises composite peptide and composite plant extract; the composite peptide is prepared from the following raw materials: oligopeptide-4, palmitoyl dipeptide-7, tripeptide-10 citrulline, and an antioxidant. The composite plant extract is prepared from the following raw materials: plumeria rubra flowers, chayote fruits and plantain fruits. Different peptides are selected to form the composite peptide raw material for improving the skin elasticity, so that multi-channel improvement on the skin elasticity can be adaptively realized, and the effect of improving the skin elasticity of a finished product is effectively improved. The white plumeria rubra flowers, the chayote fruits and the big banana fruits are blended and extracted, the active ingredients of the obtained compound extracting solution are diversified and complementary in effect, the compound extracting solution can have different effects of resisting oxidative stress reaction, removing various free radicals and reducing attack of reactive oxygen free radicals on skin collagen, and the remarkable anti-oxidation effect is achieved through the synergistic effect.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a complex peptide composition for increasing skin elasticity and its preparation method. Background Technology

[0002] Skin elasticity is key to maintaining a youthful appearance, based on a three-dimensional network of collagen, elastin fibers, and hyaluronic acid in the dermis. However, with the accumulation of oxidative stress from both internal and external factors, especially free radicals, these structural proteins are continuously attacked and degraded, disrupting the stability of the extracellular matrix and gradually leading to decreased skin elasticity, sagging, and wrinkle formation. Therefore, effectively maintaining skin elasticity requires not only replenishing or promoting the synthesis of supporting structures but also simultaneously combating oxidative damage and blocking the key factors that cause elasticity loss. Currently, most products on the market tend to focus on a single function, such as only stimulating collagen production or providing only antioxidant protection, failing to address the problem of skin elasticity loss from multiple dimensions and in a systematic way.

[0003] Therefore, it is particularly necessary to develop a composition that combines the effects of increasing skin elasticity and anti-oxidation. The aim is to strengthen the skin's elasticity support system while effectively eliminating free radicals and relieving oxidative stress, laying a solid foundation for maintaining skin elasticity in the long term. This has significant advantages over single-function products. Summary of the Invention

[0004] To address the problems existing in the prior art, the purpose of this invention is to provide a complex peptide composition that increases skin elasticity and a method for preparing the same.

[0005] The objective of this invention can be achieved through the following technical solutions: The first aspect of this invention provides a complex peptide composition for increasing skin elasticity, comprising the following components by weight: 5.9-8 parts of complex peptides and 6-10 parts of complex plant extracts.

[0006] As an example of the present invention, the raw materials of the complex peptide include: Oligopeptide-4 can enhance the transmission of growth factor signals, promote the proliferation and differentiation of fibroblasts, and increase the synthesis of elastin and collagen, thereby achieving the effects of skin renewal and improving the smoothness of skin texture. Palmitoyl dipeptide-7 is a signaling peptide that stimulates the synthesis of collagen I and collagen III. It can also increase the production of elastin, hyaluronic acid and fibronectin, increase skin elasticity, strengthen cell-matrix connections, and make the skin look more elastic and firm. Tripeptide-10 citrulline can mimic the interaction between core proteoglycans and collagen, thereby regulating the process of collagen fibrillation. It helps maintain appropriate spatial spacing and size uniformity between fibrillary fibers, enhances their stability in tissues, and thus enhances the softness and elasticity of the skin. In addition, tripeptide-10 citrulline also has moisturizing and barrier-improving functions, which can effectively improve skin hydration and slow down the loss of elasticity. The raw materials for preparing the compound plant extract include: White frangipani flower, the extract of white frangipani (PLUMERIA ALBA) flower is rich in flavonoids, phenolic compounds and other active ingredients, which can protect the skin from oxidative stress, especially resist the skin oxidative stress response caused by ultraviolet radiation. In addition, it also has a certain moisturizing effect, which can replenish and lock in the skin's moisture. Chayote fruit, the extract of chayote (SECHIUM EDULE) fruit has the ability to scavenge DPPH·, ·OH, O2. - Its ability to effectively reduce the damage caused by free radicals to the skin, as well as inhibit the peroxidation of skin lipids, reduces the risk of skin barrier damage caused by oxidation; Banana fruit contains reactive oxygen species, which are one of the main causes of skin oxidative stress and aging. The extract of banana (Musapientum) fruit is rich in flavonoids, polysaccharides and phenols, which can enhance antioxidant effects through multiple pathways. Among them, the phenolic compounds it contains can combine with metal ions on the skin surface and reduce metal-catalyzed oxidation reactions through complexation, thereby reducing the attack of reactive oxygen species on skin collagen.

[0007] As an example of the present invention, the raw materials of the complex peptide include: 2-3.8 parts by weight of oligopeptide-4, 1.8-3.5 parts by weight of palmitoyl dipeptide-7, and 0.8-1.6 parts by weight of tripeptide-10 citrulline. The raw materials for preparing the compound plant extract include: 7-13 parts by weight of frangipani flowers, 1-2 parts by weight of chayote fruit, and 5-10 parts by weight of banana fruit.

[0008] As an example of the present invention, the preparation method of the compound plant extract includes the following steps: The flowers of frangipani, the fruit of chayote, and the fruit of banana were crushed and sieved. The sieved material was mixed to obtain a mixed plant powder. The mixed plant powder was mixed with deionized water and then extracted by ultrasonic extraction under controlled temperature. The mixture was filtered, and the residue was extracted once more. The two filtrates were combined to obtain filtrate I. After filtration, filtrate II was obtained and centrifuged at a controlled speed. The supernatant was concentrated and finely filtered to obtain the compound plant extract.

[0009] As an example of the present invention, the mass ratio of the mixed plant powder to deionized water is 1:15-20.

[0010] As an example of the present invention, the sieving is performed through an 80-100 mesh sieve; the temperature-controlled ultrasonic extraction is specifically performed at a controlled temperature of 60-65℃ and an ultrasonic power of 400-420W for 1-1.5 hours; the filtration is performed using 200-mesh gauze; the speed-controlled centrifugation is performed at a speed of 4000-4500 r / min for 15-20 minutes; the concentration is performed to concentrate to 0.55-0.6 times the weight of the supernatant; and the fine filtration is performed through a 200nm ceramic membrane.

[0011] As an example of the present invention, the skin elasticity-enhancing peptide-containing composition further includes: preservatives and solvents.

[0012] As an example of the present invention, the preservative is 0.3-0.5 parts by weight; The solvent is 81.6-87.2 parts by weight.

[0013] Furthermore, the preservative includes ethylhexylglycerin.

[0014] The solvent includes: 1,2-hexanediol and deionized water.

[0015] Furthermore, the solvent comprises: 3-5 parts by weight of 1,2-hexanediol and 76.6-84.2 parts by weight of deionized water.

[0016] The second aspect of this invention provides a method for preparing a complex peptide composition that increases skin elasticity, comprising the following steps: After mixing the solvent, add the complex peptide and stir while controlling the temperature. After cooling, add the complex plant extract and preservative in sequence, stir and homogenize to obtain the finished product containing the complex peptide composition.

[0017] As an example of the present invention, the temperature of the temperature-controlled stirring is 75-80℃ and the time is 20-30min; the temperature reduction is to reduce the temperature to 40-45℃; the stirring and homogenization are to stir for 15-20min and then homogenize at a controlled speed of 2500-3000r / min for 3-5min.

[0018] The third aspect of this invention provides the application of a complex peptide composition that increases skin elasticity in the preparation of cosmetics.

[0019] The beneficial effects of this invention are: (1) Increasing skin elasticity can be achieved by increasing the synthesis of elastin and collagen, strengthening cell-matrix connections, enhancing the stability of fibrils in tissues, and improving skin hydration. The applicant has found that using oligopeptide-4, palmitoyl dipeptide-7, tripeptide-10, and citrulline as raw materials to improve skin elasticity can achieve multi-pathway improvement of skin elasticity in a suitable manner, and the effect is significantly better than the application of single peptide components, effectively improving the skin elasticity of the finished product.

[0020] (2) In this invention, the flowers of white frangipani, the fruit of chayote and the fruit of banana are extracted together. The resulting compound extract has a variety of active ingredients and complementary effects. It can have different effects such as resisting oxidative stress response, scavenging various free radicals and reducing the attack of reactive oxygen free radicals on skin collagen. It achieves significant antioxidant effects through synergistic effect. Moreover, the three raw materials are extracted together, which reduces the complicated steps of separate extraction, separate concentration and then mixing, simplifies the production process and facilitates large-scale production.

[0021] (3) The basis for maintaining skin elasticity is sufficient collagen, elastic fibers and hyaluronic acid in the extracellular matrix. Oxidative stress caused by free radicals is the key factor that destroys the support of skin elasticity, which can easily lead to accelerated degradation of collagen and elastic fibers, and eventually cause aging phenomena such as loss of skin elasticity, skin laxity and increased wrinkles. Therefore, based on the above research, the composition developed in this invention has the effect of increasing skin elasticity and also has the effect of anti-oxidation, which provides a solid foundation for maintaining skin elasticity for a long time. The effect is far superior to products with only a single function. Detailed Implementation

[0022] The technical solution of the present invention is illustrated below through specific examples. It should be understood that the one or more method steps mentioned in the present invention do not preclude the existence of other method steps before or after the combined steps, or the insertion of other method steps between these explicitly mentioned steps; it should also be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, unless otherwise stated, the numbering of each method step is merely a convenient tool for identifying each method step, and not for limiting the order of the method steps or defining the scope of the present invention. Changes or adjustments to their relative relationships, without substantially altering the technical content, should also be considered within the scope of the present invention.

[0023] To better understand the above technical solutions, exemplary embodiments of the present invention are described in more detail below. While exemplary embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the invention to those skilled in the art.

[0024] The raw materials used in this invention are all commercially available conventional products, wherein: Oligopeptide-4, purchased from Chengdu Jinglin Biotechnology Co., Ltd. Palmitoyl dipeptide-7, purchased from Jinan Guruit Chemical Co., Ltd. Tripeptide-10 citrulline, purchased from Nanjing Leon Biotechnology Co., Ltd.

[0025] The present invention will be further described below with reference to the following embodiments.

[0026] Example 1

[0027] A complex peptide composition for increasing skin elasticity, comprising the following ingredients in parts by weight: 5.9 parts of complex peptides, 8 parts of complex plant extracts, 0.3 parts of preservatives, and 85.8 parts of solvents; The raw materials for the complex peptide include: 2.9 parts by weight of oligopeptide-4, 1.8 parts by weight of palmitoyl dipeptide-7, and 1.2 parts by weight of tripeptide-10 citrulline. The raw materials for preparing the compound plant extract include: 10 parts by weight of white frangipani flowers, 2 parts by weight of chayote fruit, and 7.5 parts by weight of banana fruit. The preservative includes: ethylhexylglycerin; The solvent comprises: 4 parts by weight of 1,2-hexanediol and 81.8 parts by weight of deionized water; The preparation method of the compound plant extract includes the following steps: The flowers of frangipani, the fruit of chayote, and the fruit of banana were crushed separately and passed through an 80-mesh sieve. The sieved material was mixed to obtain a mixed plant powder. The mixed plant powder and deionized water were mixed at a mass ratio of 1:20 and extracted at 65℃ with an ultrasonic power of 400W for 1 hour. The mixture was filtered through 200-mesh gauze, and the residue was collected and extracted once more. The two filtrates were combined to obtain filtrate I. After filtration, filtrate II was obtained and centrifuged at 4200r / min for 15 minutes. The supernatant was collected and concentrated to 0.6 times its weight. The mixture was then filtered through a 200nm ceramic membrane to obtain the compound plant extract.

[0028] Example 2

[0029] A complex peptide composition for increasing skin elasticity, comprising the following ingredients in parts by weight: The compound peptides consist of 6.3 parts, the compound plant extracts consist of 6 parts, the preservatives consist of 0.5 parts, and the solvents consist of 87.2 parts. The raw materials for the complex peptide include: 2 parts by weight of oligopeptide-4, 3.5 parts by weight of palmitoyl dipeptide-7, and 0.8 parts by weight of tripeptide-10 citrulline. The raw materials for preparing the compound plant extract include: 7 parts by weight of white frangipani flowers, 1.5 parts by weight of chayote fruit, and 10 parts by weight of banana fruit. The preservative includes: ethylhexylglycerin; The solvent comprises: 3 parts by weight of 1,2-hexanediol and 84.2 parts by weight of deionized water; The preparation method of the compound plant extract includes the following steps: The flowers of frangipani, the fruit of chayote, and the fruit of banana were crushed separately and passed through a 100-mesh sieve. The sieved material was mixed to obtain a mixed plant powder. The mixed plant powder and deionized water were mixed at a mass ratio of 1:15 and extracted at 60℃ with an ultrasonic power of 420W for 1 hour. The mixture was filtered through a 200-mesh gauze. The residue was collected and the extraction was repeated once. The two filtrates were combined to obtain filtrate I. After filtration, filtrate II was obtained and centrifuged at 4000r / min for 20 minutes. The supernatant was collected and concentrated to 0.55 times its weight. The mixture was then filtered through a 200nm ceramic membrane to obtain the compound plant extract.

[0030] Example 3

[0031] A complex peptide composition for increasing skin elasticity, comprising the following ingredients in parts by weight: 8 parts of compound peptides, 10 parts of compound plant extracts, 0.4 parts of preservatives, and 81.6 parts of solvents; The raw materials for the complex peptide include: 3.8 parts by weight of oligopeptide-4, 2.6 parts by weight of palmitoyl dipeptide-7, and 1.6 parts by weight of tripeptide-10 citrulline. The raw materials for preparing the compound plant extract include: 13 parts by weight of white frangipani flowers, 1 part by weight of chayote fruit, and 5 parts by weight of banana fruit. The preservative includes: ethylhexylglycerin; The solvent comprises: 5 parts by weight of 1,2-hexanediol and 76.6 parts by weight of deionized water; The preparation method of the compound plant extract includes the following steps: The flowers of frangipani, the fruit of chayote, and the fruit of banana were crushed separately and passed through a 100-mesh sieve. The sieved material was mixed to obtain a mixed plant powder. The mixed plant powder and deionized water were mixed at a mass ratio of 1:17.5 and extracted at 63℃ with an ultrasonic power of 410W for 1.5 hours. The mixture was filtered through a 200-mesh gauze. The residue was collected and the extraction was repeated once. The two filtrates were combined to obtain filtrate I. After filtration, filtrate II was obtained and centrifuged at 4500r / min for 17.5 minutes. The supernatant was collected and concentrated to 0.58 times its weight. The mixture was then filtered through a 200nm ceramic membrane to obtain the compound plant extract.

[0032] Comparative Example 1 The difference from Example 1 is that oligopeptide-4 is not added, and the missing weight is made up with palmitoyl dipeptide-7 and tripeptide-10 citrulline in a mass ratio of 1.8:1.2, while the rest remains the same.

[0033] Comparative Example 2 The difference from Example 1 is that palmitoyl dipeptide-7 is not added, and the missing weight is made up with oligopeptide-4 and tripeptide-10 citrulline in a mass ratio of 2.9:1.2, while the rest remains the same.

[0034] Comparative Example 3 The difference from Example 1 is that tripeptide-10 citrulline is not added, and the missing weight is made up with oligopeptide-4 and palmitoyl dipeptide-7 in a mass ratio of 2.9:1.8, while the rest remains the same.

[0035] Comparative Example 4 The difference from Example 1 is that white frangipani flowers are not added to the raw materials for preparing the compound plant extract, and the missing weight is made up by adding chayote fruit and banana fruit in a mass ratio of 2:7.5, while the rest remains the same.

[0036] Comparative Example 5 The difference from Example 1 is that chayote fruit is not added to the raw materials for preparing the compound plant extract, and the missing weight is made up by frangipani flowers and banana fruit in a mass ratio of 10:7.5, with the rest remaining unchanged.

[0037] Comparative Example 6 The difference from Example 1 is that the raw materials for preparing the compound plant extract do not include banana fruit, and the missing weight is made up by using frangipani flowers and chayote fruit in a mass ratio of 10:2, while the rest remain unchanged.

[0038] Test Example 1 elastase inhibition rate test: (1) Prepare the solution: The sample solution to be tested was the complex peptide composition prepared in Examples 1-3 and Comparative Examples 1-3, with a test concentration of 3%. Tris-HCl buffer (0.1M pH=8.0): Add 2.42g Tris to 200mL of pure water, dissolve completely, and then adjust the pH to 8.0 with HCl. Positive control (1 mg / mL tea polyphenol solution): Take 0.01 g of tea polyphenol, add 10 mL of Tris-HCl buffer, mix well, and use as the positive control group; Substrate solution (2 mmol / L AAAPVN): Take 0.009 g AAAPVN (N-succinyl-alanine-alanine-alanine-nitroaniline), add 10 mL Tris-HCl buffer, mix well, and use as substrate solution; Porcine pancreatic elastase solution (0.2 U / mg): Take 0.5 mg of porcine pancreatic elastase powder (4 U / mg) and dilute it to 10 mL of Tris-HCl buffer; (2) The samples were divided into four groups: model control group, blank control group, positive control group, and sample group. Four replicates were set up for the same group and the same concentration. The amount of each solution added is shown in Table 1. Table 1

[0039] (3) Measurement methods and calculations: The reaction was carried out at 25°C for 15 minutes, and the absorbance was measured at 420 nm using an ELISA reader. Calculate the elastase inhibition rate (%) using the following formula: Elastase inhibition rate (%) = 1 - (A1 - A0) / (A2 - A0); In the formula: A0 is the blank control group; A1 is the sample group to be tested; A2 is the model control group; The final results are shown in Table 2.

[0040] Table 2

[0041] As can be seen from Table 2, the present invention uses oligopeptide-4, palmitoyl dipeptide-7 and tripeptide-10 citrulline in combination, which can adaptively and synergistically improve skin elasticity through multiple pathways, effectively enhancing the skin elasticity-enhancing efficacy of the finished product.

[0042] Test Example 2 The peptide-containing compositions prepared in Examples 1-3 and Comparative Examples 4-6 were added to deionized water to prepare 3wt% test solutions. 3.0 mL of the test solution was transferred to a 10 mL colorimetric tube, and 3.0 mL of 0.15 mM DPPH methanol solution was added. The mixture was shaken well and reacted at room temperature in the dark for 30 min. The absorbance value A1 was measured at 517 nm. Under the same conditions, methanol was used instead of DPPH solution to measure the background absorbance value A2. The sample solvent was used instead of the sample solvent to measure the blank value A0.

[0043] DPPH clearance rate (%) = (A0 - (A1 - A2)) / A0 × 100%.

[0044] The final results are shown in Table 3.

[0045] Table 3

[0046] As can be seen from Table 3, the present invention extracts a compound extract by blending frangipani flowers, chayote fruit and banana fruit. The resulting compound extract has diverse active ingredients and complementary effects, and can achieve significant antioxidant effects through synergistic action.

[0047] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. The illustrative expressions of the above terms in this specification should not be construed as necessarily referring to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. In addition, those skilled in the art can combine and integrate the different embodiments or examples described in this specification.

[0048] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A complex peptide composition for increasing skin elasticity, characterized in that, By weight, it includes the following ingredients: 5.9-8 parts of complex peptides, 6-10 parts of complex plant extracts; The raw materials for the complex peptide include: oligopeptide-4, palmitoyl dipeptide-7, tripeptide-10, and citrulline. The raw materials for preparing the compound plant extract include: frangipani flowers, chayote fruit, and banana fruit.

2. The skin elasticity-enhancing composition containing complex peptides according to claim 1, characterized in that, The raw materials for the complex peptide include: 2-3.8 parts by weight of oligopeptide-4, 1.8-3.5 parts by weight of palmitoyl dipeptide-7, and 0.8-1.6 parts by weight of tripeptide-10 citrulline. The raw materials for preparing the compound plant extract include: 7-13 parts by weight of frangipani flowers, 1-2 parts by weight of chayote fruit, and 5-10 parts by weight of banana fruit.

3. The skin elasticity-enhancing composition containing complex peptides according to claim 1, characterized in that, The preparation method of the compound plant extract includes the following steps: The flowers of frangipani, the fruit of chayote, and the fruit of banana were crushed and sieved. The sieved material was mixed to obtain a mixed plant powder. The mixed plant powder was mixed with deionized water and then extracted by ultrasonic extraction under controlled temperature. The mixture was filtered, and the residue was extracted once more. The two filtrates were combined to obtain filtrate I. After filtration, filtrate II was obtained and centrifuged at a controlled speed. The supernatant was concentrated and finely filtered to obtain the compound plant extract.

4. The skin elasticity-enhancing composition containing complex peptides according to claim 3, characterized in that, The mass ratio of the mixed plant powder to deionized water is 1:15-20.

5. The skin elasticity-enhancing composition containing complex peptides according to claim 3, characterized in that, The sieving is performed through an 80-100 mesh sieve; the temperature-controlled ultrasonic extraction is specifically performed at a controlled temperature of 60-65℃ and an ultrasonic power of 400-420W for 1-1.5 hours; the filtration is performed using 200-mesh gauze; the speed-controlled centrifugation is performed at 4000-4500 r / min for 15-20 minutes; the concentration is performed to concentrate to 0.55-0.6 times the weight of the supernatant; and the fine filtration is performed through a 200nm ceramic membrane.

6. The skin elasticity-enhancing composition containing complex peptides according to claim 1, characterized in that, The skin elasticity-enhancing peptide-containing composition further includes: 0.3-0.5 parts by weight of preservative and 81.6-87.2 parts by weight of solvent.

7. The skin elasticity-enhancing composition containing complex peptides according to claim 1, characterized in that, The preservative includes: ethylhexylglycerin; The solvent comprises: 3-5 parts by weight of 1,2-hexanediol and 76.6-84.2 parts by weight of deionized water.

8. A method for preparing a skin-elasticity-enhancing complex peptide composition as described in any one of claims 6-7, characterized in that, Includes the following steps: After mixing the solvent, add the complex peptide and stir while controlling the temperature. After cooling, add the complex plant extract and preservative in sequence, stir and homogenize to obtain the finished product containing the complex peptide composition.

9. The method for preparing the skin elasticity-enhancing complex peptide composition according to claim 8, characterized in that, The temperature for temperature-controlled stirring is 75-80℃ for 20-30 minutes; the temperature drop is to 40-45℃; the stirring and homogenization are to stir for 15-20 minutes and then homogenize at a controlled speed of 2500-3000 r / min for 3-5 minutes.

10. The use of a skin elasticity-enhancing complex peptide composition as described in any one of claims 6-7 in the preparation of cosmetics.

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