Immune cell amplification incubator

Abstract

The utility model relates to the technical field of incubators, in particular to an immune cell amplification incubator. According to the technical scheme, the culture box comprises a culture box body, a box door is hinged to the outer wall of one side of the culture box body, the culture box further comprises a base layer fixedly connected to the bottom of the culture box body, a mounting frame is fixedly mounted on the bottom wall of the culture box body, and a limiting plate is fixedly mounted between the inner walls of the culture box body; and two guide shafts are rotationally mounted between the limiting plate and the mounting frame. According to the utility model, the immune cells with an accumulation phenomenon can be automatically stirred and dispersed, the non-uniform growth of the cells or the formation of aggregates can be avoided, and the normal growth of the immune cells can be better facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of incubators, in particular to an immune cell amplification incubator. Background Art

[0002] There are various cells in the human body, and immune cells are a very important part of them. Immune cells include lymphocytes, dendritic cells, granulocytes, mast cells, etc., and each cell has its own important function. Some cells can phagocytize cells, and some cells can recognize cells. The emergence of immune cell amplification technology can enable the human body to recognize and phagocytize viruses more quickly after being invaded by external viruses. In the development of this immune cell amplification technology, the cell culture box is an indispensable part.

[0003] Cells cultured in an immune cell expansion incubator may aggregate, which refers to the phenomenon that cells aggregate into clumps or groups during the culture process. Under normal circumstances, cells should be dispersed individually in the culture medium, but in some cases, cells may aggregate for various reasons. When cells aggregate, personnel are required to open the incubator and stir manually to avoid uneven cell growth or formation of aggregates, which affects cell growth and function. However, during the manual stirring process, dust and bacteria will take the opportunity to enter the incubator and affect the growth and development of cells. Therefore, the present application proposes an immune cell expansion incubator. Summary of the invention

[0004] The utility model aims to provide an immune cell proliferation incubator to solve the problem that cells aggregate and are difficult to stir in the background technology.

[0005] The technical solution of the utility model: an immune cell expansion incubator, comprising a incubator body, a door hingedly connected to one side outer wall of the incubator body, and further comprising:

[0006] A base layer fixedly connected to the bottom of the culture box body, a mounting frame fixedly installed on the bottom wall of the culture box body, a limit plate fixedly installed between the inner walls of the culture box body, two guide shafts rotatably installed between the limit plate and the mounting frame, a moving plate slidably sleeved on the guide shaft, and culture dishes are arranged on both sides of the top of the moving plate;

[0007] A moving device, the moving device is arranged on the mounting frame and is used for moving the culture dish up and down;

[0008] A stirring device is arranged on the top inner wall of the culture box body and is used for stirring and dispersing the aggregated cells in the culture dish.

[0009] Optionally, the moving device includes a screw rod rotatably installed between the mounting frame and the limiting plate, the moving plate and the screw rod are threadedly connected, one end of the screw rod passes through the bottom of the mounting frame and is located in the base layer, and a driving device is provided in the base layer.

[0010] Optionally, the driving device includes a first gear fixedly mounted on one end of the screw rod, a second gear meshing on the first gear, a rotating shaft rotatably mounted on one side of the base layer, a screw cap fixedly connected to one end of the rotating shaft, and the other end of the rotating shaft fixedly connected to the second gear.

[0011] Optionally, the stirring device includes an electric slide rail fixedly mounted on the top inner wall of the culture box body, a slider is slidably mounted in the electric slide rail, a fixed plate is fixedly mounted on the bottom of the slider, a drive box is fixedly mounted on one side of the bottom of the fixed plate, a first rotating roller is rotatably mounted on the bottom of the drive box, a second rotating roller is rotatably mounted on the other side of the bottom of the fixed plate, a turntable is connected to the bottom of the second rotating roller and the first rotating roller, a plurality of stirring rods are arranged on the turntable, and a transmission device is arranged in the drive box.

[0012] Optionally, the transmission device includes a driving wheel fixedly mounted on one end of the first rotating roller, a driven wheel fixedly mounted on the second rotating roller, the driven wheel and the driving wheel are connected via a belt drive, a driving motor is fixedly mounted in the driving box, and the output shaft of the driving motor is fixedly connected to the driving wheel.

[0013] Optionally, two observation windows are provided on the top of the culture box body, and a control panel is provided on one side of the culture box body.

[0014] Compared with the prior art, the utility model has the following beneficial technical effects:

[0015] 1. By setting a screw, a guide shaft, a moving plate, a first gear, a second gear and a rotating shaft, twisting the screw cap to rotate the rotating shaft, so that the second gear drives the first gear to rotate, and finally the screw is rotated, so that the moving plate can be moved up and down for adjustment, which is convenient for moving the culture dish up to observe whether there is aggregation phenomenon, and can also move the immune cells to a position suitable for growth, which is highly practical;

[0016] 2. By setting a first rotating roller, a second rotating roller, a turntable, a stirring rod, an electric slide rail and a slider, the electric slide rail is controlled by a control panel so that the slider can drive the stirring rod to move back and forth in the culture dish, and then the first rotating roller and the second rotating roller are driven by the drive wheel to rotate together to stir the cells gathered in the culture dish, thereby avoiding uneven cell growth or formation of aggregates, which affects the growth and function of the cells.

[0017] The utility model realizes automatic stirring and dispersion of immune cells that have accumulated, avoids uneven cell growth or formation of aggregates, and is more conducive to the normal growth of immune cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A structural schematic diagram of an embodiment of the utility model is given;

[0019] Figure 2 A schematic diagram of the internal structure of a culture box according to an embodiment of the utility model is given;

[0020] Figure 3 A schematic diagram of a culture dish connection structure according to an embodiment of the utility model is given;

[0021] Figure 4 A schematic diagram of the internal structure of the base layer of an embodiment of the utility model is given;

[0022] Figure 5 The figure is a schematic diagram of the stirring rod connection structure of an embodiment of the utility model.

[0023] Figure numerals: 1. culture box body; 2. box door; 3. base layer; 4. mounting frame; 5. limit plate; 6. guide shaft; 7. movable plate; 8. culture dish; 9. screw; 10. first gear; 11. second gear; 12. rotating shaft; 13. screw cap; 14. electric slide rail; 15. slider; 16. fixed plate; 17. drive box; 18. first rotating roller; 19. second rotating roller; 20. driving wheel; 21. driven wheel; 22. driving motor; 23. turntable; 24. stirring rod; 25. control panel; 26. observation window. DETAILED DESCRIPTION

[0024] The technical solution of the present utility model will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present utility model, rather than all of the embodiments.

[0025] The components of the embodiments of the present invention generally described and shown in the drawings herein can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0026] Based on the embodiments of the present utility model, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present utility model.

[0027] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0028] It should be noted that the terms "include", "comprises" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. Example

[0030] like Figure 1 As shown, the utility model proposes an immune cell expansion incubator, comprising a incubator body 1, a door 2 is hingedly connected to one side outer wall of the incubator body 1, a handle is fixedly installed on the door 2, and the door 2 is opened and closed by pulling the handle, and a sealing layer is connected to the door 2 and the inner wall of the incubator body 1;

[0031] In addition, two observation windows 26 are provided on the top of the culture box 1, and a control panel 25 is provided on one side of the culture box 1. The control panel 25 can control the humidity, temperature, etc. inside the culture box 1. In order to facilitate the growth and development of the immune cells inside the culture box 1, the growth of cells inside the culture box 1 can be observed through the observation window 26.

[0032] like Figure 2 and Figure 3As shown, the device also includes a base layer 3 fixedly connected to the bottom of the culture box body 1, a mounting frame 4 is fixedly installed on the bottom wall of the culture box body 1, a limit plate 5 is fixedly installed between the inner walls of the culture box body 1, two guide shafts 6 are rotatably installed between the limit plate 5 and the mounting frame 4, and a moving plate 7 is slidably sleeved on the guide shaft 6. The guide shaft 6 plays a guiding role, so that the moving plate 7 can slide up and down on the guide shaft 6;

[0033] In addition, culture dishes 8 are arranged on both sides of the top of the movable plate 7. The culture dishes 8 are used to culture and grow immune cells to proliferate and develop the immune cells. A heating plate is installed at the bottom of the culture dish 8, and the heating of the heating plate is controlled by the control panel 25.

[0034] like Figure 2-4 As shown, the device also includes a moving device, which is arranged on the mounting frame 4 for moving the culture dish 8 up and down. The moving device includes a screw rod 9 rotatably mounted between the mounting frame 4 and the limiting plate 5. The moving plate 7 and the screw rod 9 are threadedly connected. One end of the screw rod 9 passes through the bottom of the mounting frame 4 and is located in the base layer 3. The culture dish 8 can be adjusted and moved up and down by rotating the screw rod 9 and guiding the guide shaft 6.

[0035] In addition, a driving device is provided in the base layer 3, and the driving device includes a first gear 10 fixedly installed at one end of the screw 9, and a second gear 11 is meshed on the first gear 10. A rotating shaft 12 is rotatably installed on one side of the base layer 3, and a screw cap 13 is fixedly connected to one end of the rotating shaft 12, and the other end of the rotating shaft 12 is fixedly connected to the second gear 11. The screw cap 13 is turned to rotate the rotating shaft 12, so that the second gear 11 drives the first gear 10 to rotate, and further rotates the screw 9.

[0036] like Figure 2 and Figure 5 As shown, the device also includes a stirring device, which is arranged on the top inner wall of the culture box body 1 for stirring and dispersing the aggregated cells in the culture dish 8. The stirring device includes an electric slide rail 14 fixedly installed on the top inner wall of the culture box body 1, and a slider 15 is slidably installed in the electric slide rail 14. The electric slide rail 14 is controlled by a control panel 25 so that the slider 15 can move forward and backward on the electric slide rail 14. A fixed plate 16 is fixedly installed at the bottom of the slider 15, and a driving box 17 is fixedly installed on one side of the bottom of the fixed plate 16;

[0037] Furthermore, a first rotating roller 18 is rotatably mounted on the bottom of the driving box 17, and a second rotating roller 19 is rotatably mounted on the other side of the bottom of the fixed plate 16. A turntable 23 is connected to the bottoms of the second rotating roller 19 and the first rotating roller 18. The rotation of the first rotating roller 18 and the second rotating roller 19 drives the rotation of the turntable 23. A plurality of stirring rods 24 are arranged on the turntable 23. The stirring rods 24 are used to stir and disperse the aggregated cells.

[0038] Furthermore, a transmission device is provided in the drive box 17, and the transmission device includes a driving wheel 20 fixedly mounted on one end of the first rotating roller 18, a driven wheel 21 fixedly mounted on the second rotating roller 19, the driven wheel 21 and the driving wheel 20 are connected by belt transmission, and a driving motor 22 is fixedly mounted in the drive box 17, and the output shaft of the driving motor 22 is fixedly connected to the driving wheel 20. When the driving motor 22 is started, the driving wheel 20 rotates, and the driven wheel 21 rotates at the same time, so that the first rotating roller 18 and the second rotating roller 19 rotate synchronously, so that the stirring rod 24 stirs the cells gathered in the culture dish 8.

[0039] Working principle: The growth of immune cells in the culture dish 8 inside the culture box 1 can be observed through the observation window 26. When an aggregation problem is found, first twist the screw cap 13 to rotate the shaft 12. The rotation of the shaft 12 drives the second gear 11 to rotate, and at the same time, the first gear 10 is rotated, and the screw 9 is rotated. Under the guidance of the guide shaft 6, the culture dish 8 moves upward, so that the stirring rod 24 can be inserted into the culture dish 8 and stop, and the drive motor 22 is started to make the active wheel 20 drive the driven wheel 21 to rotate synchronously, and at the same time, the first rotating roller 18 and the second rotating roller 19 are rotated, so that the turntable 23 drives the stirring rod 24 to stir the cells aggregated in the culture dish, and the electric slide rail 14 is controlled by the control panel 25, so that the slider 15 drives the fixed plate 16 to move back and forth, so as to realize automatic stirring of the aggregated cells in the entire culture dish 8, thereby avoiding uneven cell growth or formation of aggregates, which affects the growth and function of cells.

[0040] The above-mentioned specific embodiments are only several optional embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. An immune cell expansion incubator, comprising a incubator body (1), wherein a door (2) is hingedly connected to an outer wall of one side of the incubator body (1), characterized in that: Also includes: A base layer (3) is fixedly connected to the bottom of the culture box (1); a mounting frame (4) is fixedly mounted on the bottom wall of the culture box (1); a limit plate (5) is fixedly mounted between the inner walls of the culture box (1); two guide shafts (6) are rotatably mounted between the limit plate (5) and the mounting frame (4); a movable plate (7) is slidably sleeved on the guide shaft (6); and culture dishes (8) are arranged on both sides of the top of the movable plate (7); A moving device, the moving device being arranged on the mounting frame (4) and used for moving the culture dish (8) up and down; A stirring device, wherein the stirring device is arranged on the top inner wall of the culture box (1) and is used for stirring and dispersing the aggregated cells in the culture dish (8).

2. The immune cell expansion incubator according to claim 1, characterized in that: The moving device comprises a screw rod (9) rotatably mounted between the mounting frame (4) and the limiting plate (5); the moving plate (7) and the screw rod (9) are threadedly connected; one end of the screw rod (9) passes through the bottom of the mounting frame (4) and is located in the base layer (3); and a driving device is provided in the base layer (3).

3. An immune cell expansion incubator according to claim 2, characterized in that: The driving device comprises a first gear (10) fixedly mounted on one end of the screw rod (9), a second gear (11) meshing with the first gear (10), a rotating shaft (12) rotatably mounted on one side of the base layer (3), a screw cap (13) fixedly connected to one end of the rotating shaft (12), and the other end of the rotating shaft (12) fixedly connected to the second gear (11).

4. The immune cell expansion incubator according to claim 1, characterized in that: The stirring device comprises an electric slide rail (14) fixedly mounted on the inner wall of the top of the culture box (1), a slider (15) being slidably mounted in the electric slide rail (14), a fixed plate (16) being fixedly mounted at the bottom of the slider (15), a drive box (17) being fixedly mounted on one side of the bottom of the fixed plate (16), a first rotating roller (18) being rotatably mounted at the bottom of the drive box (17), a second rotating roller (19) being rotatably mounted at the other side of the bottom of the fixed plate (16), a rotating disk (23) being connected to the bottom of the second rotating roller (19) and the first rotating roller (18), a plurality of groups of stirring rods (24) being arranged on the rotating disk (23), and a transmission device being arranged in the drive box (17).

5. The immune cell expansion incubator according to claim 4, characterized in that: The transmission device comprises a driving wheel (20) fixedly mounted on one end of a first rotating roller (18); a driven wheel (21) fixedly mounted on the second rotating roller (19); the driven wheel (21) and the driving wheel (20) are connected via a belt transmission; a driving motor (22) is fixedly mounted in the driving box (17); an output shaft of the driving motor (22) is fixedly connected to the driving wheel (20).

6. The immune cell expansion incubator according to claim 1, characterized in that: Two observation windows (26) are provided on the top of the culture box (1), and a control panel (25) is provided on one side of the culture box (1).

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