Bacillus subtilis bacterial liquid low-temperature concentration device

Abstract

The utility model discloses a bacillus subtilis bacterial liquid low-temperature concentration device, which relates to the technical field of bacillus subtilis bacterial liquid concentration, and comprises a table body, the outer wall of the top of the table body is fixedly connected with a table plate, the top of the table plate is provided with a vacuum pump, one side of the vacuum pump is connected with a connecting pipe in an inserted manner, and the other side of the vacuum pump is provided with a connecting pipe. A water bath kettle is arranged at the top of the table body, a containing bottle is arranged in the water bath kettle, hot water is contained in the water bath kettle, and the temperature of the hot water ranges from 50 DEG C to 60 DEG C. A control panel is fixedly connected to the outer wall of one side of the table body, and a limiting assembly used for fixing the containing bottle is arranged at the top of the water bath kettle. A condensation assembly is arranged on the top of the platen. According to the device disclosed by the utility model, air in the containing bottle is pumped out to enable the interior of the containing bottle to be in a vacuum state, so that the boiling point of water in the bacillus subtilis liquid can be effectively reduced, and water vapor can still be evaporated when the bacillus subtilis liquid is subjected to low-temperature concentration subsequently.

Description

Technical Field

[0001] The utility model relates to the technical field of Bacillus subtilis bacterial liquid concentration, in particular to a Bacillus subtilis bacterial liquid low-temperature concentration device. Background Art

[0002] Bacillus subtilis is a species of Bacillus, with an elliptical to columnar shape, located in the center or slightly off the bacterial body. The bacterial body does not swell after spore formation. The surface of the colony is rough and opaque, dirty white or slightly yellow, and often forms wrinkles when growing in liquid culture medium. It is an aerobic bacterium. It can use protein, various sugars and starch to decompose tryptophan to form indole. It is widely used in genetic research, and the synthesis pathway of purine nucleotides and its regulatory mechanism are well studied. It is widely distributed in soil and corrupted organic matter, and is easy to reproduce in the juice of dead grass.

[0003] When heating and concentrating Bacillus subtilis, the traditional concentration equipment often has a concentration temperature that is too high, which easily causes the Bacillus subtilis to die during concentration and affects the survival rate of the Bacillus subtilis after concentration. Therefore, a Bacillus subtilis liquid low-temperature concentration device is urgently needed to solve the above problem. Utility Model Content

[0004] The purpose of the utility model is to solve the shortcomings of the prior art and propose a low-temperature concentration device for Bacillus subtilis bacterial liquid. Its advantage is that it can realize low-temperature concentration of Bacillus subtilis bacterial liquid, effectively ensure the survival rate of Bacillus subtilis bacterial liquid after concentration, and meet people's use needs.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A Bacillus subtilis liquid low-temperature concentration device comprises a platform, a top outer wall of the platform is fixedly connected to a platform, a vacuum pump is arranged on the top of the platform, a connecting pipe is plugged into one side of the vacuum pump, a water bath is arranged on the top of the platform, a filling bottle is arranged inside the water bath, hot water is filled inside the water bath, and the temperature of the hot water is 50°C-60°C, and a control panel is fixedly connected to the outer wall of one side of the platform;

[0007] A limiting component for fixing the containing bottle is provided on the top of the water bath;

[0008] A condensation assembly is disposed on the top of the platform.

[0009] Through the above technical scheme: the low-temperature concentration of the Bacillus subtilis liquid can be achieved, the survival rate of the Bacillus subtilis liquid after concentration is effectively guaranteed, and the use needs of people are met.

[0010] The utility model is further configured such that a vertical tube is provided on the top of the containing bottle, a fixed tube is provided on the top of the vertical tube, a first butt joint tube is fixedly connected to the circumferential outer wall of the vertical tube, and an end of the connecting tube away from the vacuum pump is fixedly connected to the first butt joint tube.

[0011] Through the above technical solution: the connection between the filling bottle and the vacuum pump is achieved.

[0012] The utility model is further configured such that a fixing ring is provided on the circumferential outer wall of the vertical pipe, both ends of the fixing ring are fixedly connected with connecting plates, a through hole is opened on one side of the connecting plate, a fixing screw is inserted into the through hole, and a fixing nut is meshed on the circumferential outer wall of the fixing screw.

[0013] Through the above technical solution: the fixing ring can be locked and fixed, ensuring that the fixing ring is tightly connected to the circumferential outer wall of the vertical pipe.

[0014] The utility model is further configured such that the limit assembly includes a support rod fixedly connected to the circumferential outer wall of the fixing ring, the end of the support rod away from the fixing ring is fixedly connected to a first circular plate, the top of the water bath is fixedly connected to a second circular plate, the top of the second circular plate is provided with equidistant circularly distributed clamping holes, the bottom of the first circular plate is fixedly connected to a clamping column, and the clamping column passes through the inside of the clamping hole.

[0015] Through the above technical solution: it is ensured that the containing bottle can remain stable when being heated in a water bath.

[0016] The utility model is further configured that a drain pipe is fixedly connected to the circumferential outer wall of the water bath, and a control valve is arranged on one side of the drain pipe, the first butt joint pipe and the fixed pipe.

[0017] Through the above technical solution: it is convenient for the staff to open and close the drainage pipe, the first connecting pipe and the fixed pipe.

[0018] The utility model is further configured that the condensation component includes a condensation tube inserted into the interior of the fixed tube, a sealing plug is fixedly connected to the circumferential outer wall of the condensation tube, and the sealing plug cooperates with the fixed tube.

[0019] Through the above technical solution: the water vapor generated by evaporation can be condensed through the condenser.

[0020] The utility model is further configured that the circumferential outer wall of the condenser tube is fixedly connected with a limiting plate.

[0021] Through the above technical solution: the water bath can be fixed, thereby ensuring the stability of the water bath during condensation.

[0022] The utility model is further configured such that a water bath is fixedly connected to the top of the limit plate, the middle portion of the water bath is a hollow structure, one end of the condenser passes through the middle portion of the water bath, and the circumferential outer wall of the condenser contacts the circumferential inner wall of the water bath.

[0023] Through the above technical solution:.

[0024] The beneficial effects of the utility model are:

[0025] 1. A Bacillus subtilis liquid low-temperature concentration device is provided with a vacuum pump, a connecting pipe, a containing bottle and a vertical pipe. Before a staff member needs to heat and concentrate the Bacillus subtilis liquid, the vacuum pump can be started first. The vacuum pump extracts the air inside the containing bottle through the connecting pipe to make the inside of the containing bottle in a vacuum state, thereby effectively reducing the boiling point of the water inside the Bacillus subtilis liquid, ensuring that the water vapor can still be evaporated when the Bacillus subtilis liquid is subsequently low-temperature concentrated.

[0026] 2. A low-temperature concentration device for Bacillus subtilis liquid is provided with a water bath, a containing bottle and a limit assembly. When the inside of the containing bottle is evacuated, a worker adds hot water of 50°C-60°C into the water bath, thereby low-temperature heating and concentration of the Bacillus subtilis liquid in the containing bottle. Before the containing bottle is heated in a water bath, the containing bottle is clamped in a clamping hole through a clamping column, thereby ensuring that the containing bottle remains stable during water bath heating.

[0027] 3. A low-temperature concentration device for Bacillus subtilis liquid is provided with a condensation component. After the Bacillus subtilis liquid in the container bottle is heated at low temperature, the water vapor rises and flows into the condenser tube. At the same time, the staff heats low-temperature cold water in the water bath, and the cold water can be used to cool and condense the water vapor. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the overall structure of a low-temperature concentration device for Bacillus subtilis bacterial liquid proposed by the utility model;

[0029] Figure 2 A low-temperature concentration device for Bacillus subtilis liquid is proposed in the utility model Figure 1 A schematic diagram of the enlarged structure at A in the middle;

[0030] Figure 3 This is a schematic diagram of a semi-sectional structure of a Bacillus subtilis liquid low-temperature concentration device proposed by the utility model;

[0031] Figure 4 The utility model provides a Bacillus subtilis liquid low temperature concentration device Figure 3 A schematic diagram of the enlarged structure at B in the middle;

[0032] Figure 5 The utility model provides a Bacillus subtilis liquid low temperature concentration device Figure 3 Schematic diagram of the enlarged structure at point C in the middle.

[0033] In the figure: 1. table body; 2. table top; 3. control panel; 4. vacuum pump; 5. connecting pipe; 6. condenser; 7. water bath; 8. water bath; 9. first circular ring plate; 10. drain pipe; 11. first docking pipe; 12. vertical pipe; 13. fixing ring; 14. connecting plate; 15. fixing screw; 16. fixing pipe; 17. supporting rod; 18. filling bottle; 19. sealing plug; 20. second circular ring plate; 21. clamping column; 22. clamping hole; 23. limit plate. DETAILED DESCRIPTION

[0034] The technical solution of this patent is further described in detail below in conjunction with specific implementation methods.

[0035] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0036] In the description of this patent, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this patent.

[0037] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, it can be fixedly connected or set, or it can be detachably connected or set, or connected or set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0038] Reference Figure 1-Figure 5A low-temperature concentration device for Bacillus subtilis liquid comprises a platform 1, a top outer wall of the platform 1 is fixedly connected with a platform 2, a vacuum pump 4 is arranged on the top of the platform 2, a connecting pipe 5 is plugged into one side of the vacuum pump 4, a water bath 7 is arranged on the top of the platform 1, a filling bottle 18 is arranged inside the water bath 7, hot water is filled inside the water bath 7, and the temperature of the hot water is 50°C-60°C, and a control panel 3 is fixedly connected to the outer wall of one side of the platform 1;

[0039] The top of the water bath 7 is provided with a limiting assembly for fixing the containing bottle 18;

[0040] A condensation assembly is provided on the top of the table 2 .

[0041] Specifically, a vertical tube 12 is provided on the top of the container bottle 18, a fixed tube 16 is provided on the top of the vertical tube 12, a first docking tube 11 is fixedly connected to the circumferential outer wall of the vertical tube 12, and one end of the connecting tube 5 away from the vacuum pump 4 is fixedly connected to the first docking tube 11, thereby realizing the connection between the container bottle 18 and the vacuum pump 4.

[0042] Specifically, a fixing ring 13 is provided on the circumferential outer wall of the vertical pipe 12, and connecting plates 14 are fixedly connected to both ends of the fixing ring 13. A through hole is opened on one side of the connecting plate 14, and a fixing screw 15 is inserted into the through hole. A fixing nut is meshed with the circumferential outer wall of the fixing screw 15, which can realize the locking and fixation of the fixing ring 13.

[0043] Specifically, the limiting assembly includes a support rod 17 fixedly connected to the circumferential outer wall of the fixing ring 13, the end of the support rod 17 away from the fixing ring 13 is fixedly connected to the first circular plate 9, the top of the water bath pot 7 is fixedly connected to the second circular plate 20, the top of the second circular plate 20 is provided with equidistant circularly distributed clamping holes 22, the bottom of the first circular plate 9 is fixedly connected to the clamping column 21, the clamping column 21 passes through the inside of the clamping hole 22, thereby ensuring that the container 18 can remain stable during water bath heating.

[0044] Specifically, the circumferential outer wall of the water bath 7 is fixedly connected with a drain pipe 10, and control valves are provided on one side of the drain pipe 10, the first butt joint pipe 11, and the fixed pipe 16 to facilitate the staff to open and close the drain pipe 10, the first butt joint pipe 11, and the fixed pipe 16.

[0045] Specifically, the condensation component includes a condensation tube 6 inserted into the fixed tube 16, and a sealing plug 19 is fixedly connected to the circumferential outer wall of the condensation tube 6. The sealing plug 19 cooperates with the fixed tube 16, and the water vapor generated by evaporation can be condensed through the condensation tube 6.

[0046] Specifically, the circumferential outer wall of the condenser tube 6 is fixedly connected to the limiting plate 23 , so as to facilitate the fixing of the water bath 8 .

[0047] Specifically, the top of the limiting plate 23 is fixedly connected to the water bath tube 8, the middle part of the water bath tube 8 is a hollow structure, one end of the condenser tube 6 passes through the middle part of the water bath tube 8, and the circumferential outer wall of the condenser tube 6 is in contact with the circumferential inner wall of the water bath tube 8, so that the water bath tube 8 can be fixed, thereby ensuring the stability of the water bath tube 8 during condensation.

[0048] Working principle: before heating the container 18 in a water bath, the staff connects the clamping column 21 to the inside of the clamping hole 22, so as to ensure that the container 18 remains stable during the water bath heating, and then starts the vacuum pump 4. The vacuum pump 4 extracts the air inside the container 18 through the connecting pipe 5 to make the inside of the container 18 in a vacuum state, thereby effectively reducing the boiling point of the water inside the Bacillus subtilis liquid, ensuring that the water vapor can still be evaporated when the Bacillus subtilis liquid is subsequently low-temperature concentrated. After the inside of the container 18 is evacuated, the staff adds 50℃-60℃ hot water into the water bath 7, so as to heat and concentrate the Bacillus subtilis liquid in the container 18 at a low temperature. After the Bacillus subtilis liquid in the container 18 is low-temperature heated, the water vapor rises and flows into the condenser 6. At the same time, the staff heats low-temperature cold water into the water bath 8, and the cold water can be used to cool and condense the water vapor.

[0049] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A Bacillus subtilis liquid low temperature concentration device, comprising a platform (1), characterized in that: The top outer wall of the platform (1) is fixedly connected to a platform (2), a vacuum pump (4) is arranged on the top of the platform (2), a connecting pipe (5) is plugged into one side of the vacuum pump (4), a water bath (7) is arranged on the top of the platform (1), a filling bottle (18) is arranged inside the water bath (7), and hot water is filled inside the water bath (7), and the temperature of the hot water is 50°C-60°C, and a control panel (3) is fixedly connected to the outer wall of one side of the platform (1); A limiting component for fixing the containing bottle (18) is provided on the top of the water bath (7); A condensation component is arranged on the top of the platform (2).

2. The low-temperature concentration device for Bacillus subtilis liquid according to claim 1, characterized in that: A vertical tube (12) is arranged on the top of the containing bottle (18), a fixed tube (16) is arranged on the top of the vertical tube (12), a first butt joint tube (11) is fixedly connected to the circumferential outer wall of the vertical tube (12), and one end of the connecting tube (5) away from the vacuum pump (4) is fixedly connected to the first butt joint tube (11).

3. The low-temperature concentration device for Bacillus subtilis liquid according to claim 2, characterized in that: A fixing ring (13) is provided on the circumferential outer wall of the vertical pipe (12), and connecting plates (14) are fixedly connected to both ends of the fixing ring (13). A through hole is opened on one side of the connecting plate (14), and a fixing screw (15) is inserted into the through hole. A fixing nut is meshed on the circumferential outer wall of the fixing screw (15).

4. The low-temperature concentration device for Bacillus subtilis liquid according to claim 3, characterized in that: The limiting assembly comprises a support rod (17) fixedly connected to the circumferential outer wall of the fixing ring (13); one end of the support rod (17) away from the fixing ring (13) is fixedly connected to a first annular plate (9); the top of the water bath (7) is fixedly connected to a second annular plate (20); the top of the second annular plate (20) is provided with equidistantly distributed circular clamping holes (22); the bottom of the first annular plate (9) is fixedly connected to a clamping column (21); the clamping column (21) passes through the inside of the clamping hole (22).

5. The low-temperature concentration device for Bacillus subtilis liquid according to claim 4, characterized in that: A drainage pipe (10) is fixedly connected to the circumferential outer wall of the water bath (7), and a control valve is provided on one side of the drainage pipe (10), the first butt joint pipe (11), and the fixed pipe (16).

6. The low-temperature concentration device for Bacillus subtilis liquid according to claim 5, characterized in that: The condensation assembly comprises a condensation tube (6) inserted into the interior of the fixed tube (16); a sealing plug (19) is fixedly connected to the circumferential outer wall of the condensation tube (6); and the sealing plug (19) cooperates with the fixed tube (16).

7. The low-temperature concentration device for Bacillus subtilis liquid according to claim 6, characterized in that: The circumferential outer wall of the condenser tube (6) is fixedly connected to a limiting plate (23).

8. The low-temperature concentration device for Bacillus subtilis liquid according to claim 7, characterized in that: The top of the limiting plate (23) is fixedly connected to a water bath (8), the middle of the water bath (8) is a hollow structure, one end of the condenser tube (6) passes through the middle of the water bath (8), and the circumferential outer wall of the condenser tube (6) is in contact with the circumferential inner wall of the water bath (8).

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