Multifunctional stem cell exosome extraction device

Abstract

The utility model discloses a kind of multifunctional stem cell exosome extraction device, it is related to stem cell exosome extraction technical field. Including mounting bracket, the inner wall of mounting bracket is fixedly connected with extraction box, further including separation extraction component, the inner wall of mounting bracket is set to separation extraction component, for separating extraction to stem cell exosome and impurity, by the monitoring component of being set, so that it is convenient to real-time capture the sedimentation trajectory of exosome and impurity in extraction box, to adjust the rotation speed of extraction box according to sedimentation trajectory, improve the separation efficiency of exosome separation, by the linkage component of being set, so that it is convenient to link separation extraction component and monitoring component, so that monitoring component carries out dynamic monitoring to exosome in extraction box, to improve monitoring precision, by the drive component of being set, so that it is convenient to drive and control linkage component, and then improve the stability of exosome separation extraction.

Description

Technical Field

[0001] This utility model relates to the field of stem cell exosome extraction technology, specifically a multifunctional stem cell exosome extraction device. Background Technology

[0002] Stem cell exosomes, rich in bioactive molecules, have shown great potential in regenerative medicine, disease diagnosis, and other fields. The quality of their extraction directly affects the effectiveness of subsequent research and applications. However, stem cell exosomes from different sources (such as bone marrow and adipose tissue) differ in characteristics such as particle size and density, highlighting the increasing demand for multifunctional and adaptable extraction devices.

[0003] However, existing extraction devices suffer from "gradient loss" during sample pretreatment. Stem cell samples often contain impurities such as cell debris and protein aggregates. Traditional extraction devices struggle to adjust in real time based on the dynamic sedimentation differences between impurities and exosomes when separating and extracting stem cells. When the density of exosomes and impurities is close, co-precipitation can easily occur, leading to the loss of the target substance. Furthermore, during multiple centrifugation switching processes, the sample is exposed to the external environment for a long time, and the activity of exosomes is easily affected by temperature fluctuations and reduced. Therefore, a multifunctional stem cell exosome extraction device is urgently needed to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a multifunctional stem cell exosome extraction device to solve the problem mentioned in the background art that traditional extraction devices are difficult to adjust in real time according to the dynamic sedimentation difference between impurities and exosomes when separating and extracting stem cells.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a multifunctional stem cell exosome extraction device, comprising a mounting frame, wherein an extraction box is fixedly connected to the inner wall of the mounting frame, and further comprising: A separation and extraction component is disposed on the inner wall of the mounting frame and is used to separate and extract stem cell exosomes from impurities; A monitoring component is disposed on the inner wall of the extraction box for real-time monitoring of stem cell exosome raw materials inside the extraction box. A linkage component, which is disposed on the side wall of the mounting frame, is used to link the separation and extraction component with the monitoring component. A drive component, which is disposed on the top surface of the mounting bracket, is used to drive the linkage component; A heat preservation component is disposed on the surface of the extraction chamber and is used to keep the exosomes inside the extraction chamber warm.

[0006] Preferably, the separation and extraction assembly includes a feeding box, which is rotatably connected to the inner wall of the extraction chamber. A plurality of separation cores are fixedly connected to the inner wall of the feeding box, and a plurality of filter holes are opened on the surface of the separation cores. A feeding pipe is fixedly connected to the inner wall of the feeding box, and the surface of the feeding pipe is rotatably connected to the inner wall of the extraction chamber.

[0007] Preferably, the monitoring component includes a rotating tube, which is rotatably connected to the inner wall of the extraction box. A laser particle size sensor is fixedly connected to the inner wall of the rotating tube, and a highly transparent glass protective cover is fixedly connected to the upper end of the rotating tube.

[0008] Preferably, the linkage component includes a linkage column, which is rotatably connected to the side wall of the mounting frame. Two first synchronous pulleys are fixedly connected to the surface of the linkage column, and a third synchronous pulley is fixedly connected to the surface of both the rotating tube and the feed tube. The surface of the third synchronous pulley and the surface of the first synchronous pulley are fitted with the same synchronous belt.

[0009] Preferably, the drive assembly includes a fixed frame, which is fixedly connected to the top surface of the mounting frame. A motor is fixedly connected to the top surface of the fixed frame. The surface of the linkage column is fixedly connected to the output end of the motor. A controller is fixedly connected to the top surface of the mounting frame. The controller is electrically connected to the motor and to the laser particle size sensor.

[0010] Preferably, the heat insulation component includes a mounting shell, which is fixedly connected to the surface of the extraction box. The inner wall of the mounting shell is provided with heat insulation cotton, which is in contact with the surface of the extraction box.

[0011] Compared with the prior art, the beneficial effects of this utility model are: The separation and extraction components facilitate the filtration and extraction of exosome raw materials, achieving precise separation of impurities and exosomes. The monitoring components enable real-time capture of the settling trajectory of exosomes and impurities inside the extraction chamber, allowing adjustment of the chamber's rotation speed based on the settling trajectory to improve exosome separation efficiency. The linkage components facilitate linkage between the separation and extraction components and the monitoring components, enabling dynamic monitoring of exosomes inside the extraction chamber and improving monitoring accuracy. The drive components facilitate the driving and control of the linkage components, thereby enhancing the stability of exosome separation and extraction. Attached Figure Description

[0012] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is a schematic diagram of the drive component structure of this utility model; Figure 3This is a schematic diagram of the linkage component structure of this utility model; Figure 4 This is a partial cross-sectional view of the separation and extraction component of this utility model.

[0013] In the diagram: 1. Mounting frame; 2. Extraction box; 3. Separation and extraction assembly; 301. Feed box; 302. Separation core; 303. Filter hole; 304. Feed pipe; 4. Monitoring assembly; 401. Rotating tube; 402. Laser particle size sensor; 403. High-transparency glass protective cover; 5. Linkage assembly; 501. Linkage column; 502. First synchronous pulley; 503. Third synchronous pulley; 504. Synchronous belt; 6. Drive assembly; 601. Fixing frame; 602. Motor; 603. Controller; 7. Insulation assembly; 701. Mounting shell; 702. Insulation cotton. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0015] Please see Figure 1 - Figure 4This utility model provides a multifunctional stem cell exosome extraction device, including a mounting frame 1, an extraction box 2 fixedly connected to the inner wall of the mounting frame 1, a separation and extraction component 3 disposed on the inner wall of the mounting frame 1 for separating and extracting stem cell exosomes from impurities, a monitoring component 4 disposed on the inner wall of the monitoring component 4 for real-time monitoring of the stem cell exosome raw material inside the extraction box 2, a linkage component 5 disposed on the side wall of the mounting frame 1 for linkage between the separation and extraction component 3 and the monitoring component 4, a drive component 6 disposed on the top surface of the mounting frame 1 for driving the linkage component 5, and a heat preservation component 7 disposed on the surface of the extraction box 2 for... The exosomes inside the extraction chamber 2 are kept at a constant temperature. The separation and extraction component 3 facilitates the filtration and extraction of exosome raw materials, achieving precise separation of impurities and exosomes. The monitoring component 4 allows for real-time capture of the sedimentation trajectory of exosomes and impurities inside the extraction chamber 2, thereby adjusting the rotation speed of the extraction chamber 2 based on the sedimentation trajectory to improve the separation efficiency of exosomes. The linkage component 5 allows for linkage between the separation and extraction component 3 and the monitoring component 4, enabling the monitoring component 4 to dynamically monitor the exosomes inside the extraction chamber 2, thereby improving monitoring accuracy. The drive component 6 facilitates the driving and control of the linkage component 5, thereby improving the stability of exosome separation and extraction.

[0016] Furthermore, the separation and extraction component 3 includes a feed box 301, which is rotatably connected to the inner wall of the extraction chamber 2. Multiple separation cores 302 are fixedly connected to the inner wall of the feed box 301. Multiple filter holes 303 are opened on the surface of the separation cores 302. A feed pipe 304 is fixedly connected to the inner wall of the feed box 301. The surface of the feed pipe 304 is rotatably connected to the inner wall of the extraction chamber 2. Through the separation and extraction component 3, the feed box 301 rotates, causing the multiple separation cores 302 to rotate. The rotation of the multiple separation cores 302 generates centrifugal force, which causes the exosomes to pass through the filter holes 303 and filter impurities onto the inner wall of the separation cores 302. This facilitates the filtration and extraction of exosome raw materials and achieves precise separation of impurities and exosomes.

[0017] Furthermore, the monitoring component 4 includes a rotating tube 401, which is rotatably connected to the inner wall of the extraction chamber 2. A laser particle size sensor 402 is fixedly connected to the inner wall of the rotating tube 401, and a high-transparency glass protective cover 403 is fixedly connected to the upper end of the rotating tube 401. Through the monitoring component 4, it is convenient to capture the sedimentation trajectory of exosomes and impurities inside the extraction chamber 2 in real time through the laser particle size sensor 402, thereby adjusting the rotation speed of the extraction chamber 2 according to the sedimentation trajectory and improving the separation efficiency of exosome separation.

[0018] Furthermore, the linkage component 5 includes a linkage column 501, which is rotatably connected to the side wall of the mounting frame 1. Two first synchronous wheels 502 are fixedly connected to the surface of the linkage column 501. Third synchronous wheels 503 are fixedly connected to the surface of the rotating tube 401 and the surface of the feed tube 304. The surface of the third synchronous wheel 503 and the surface of the first synchronous wheel 502 are fitted with the same synchronous belt 504. Through the linkage component 5, it is easy to link the separation and extraction component 3 with the monitoring component 4, so that when the feed box 301 rotates, it drives the rotating tube 401 and the laser particle size sensor 402 to rotate synchronously. This facilitates the laser particle size sensor 402 to dynamically monitor the exosomes inside the extraction box 2 and improves the monitoring accuracy.

[0019] Furthermore, the drive assembly 6 includes a fixed frame 601, which is fixedly connected to the top surface of the mounting frame 1. A motor 602 is fixedly connected to the top surface of the fixed frame 601. The surface of the linkage column 501 is fixedly connected to the output end of the motor 602. A controller 603 is fixedly connected to the top surface of the mounting frame 1. The controller 603 is electrically connected to the motor 602 and to the laser particle size sensor 402. Through the drive assembly 6, the output end of the fixed frame 601 rotates, causing the linkage column 501 to rotate. The rotation of the linkage column 501 drives the separation and extraction assembly 3 and the monitoring assembly 4 to rotate synchronously, thereby facilitating the driving and control of the rotation speed of the separation and extraction assembly 3 and the monitoring assembly 4. At the same time, through the controller 603, the laser particle size sensor 402 transmits the monitored data to the controller 603. The controller 603 then automatically controls the rotation speed of the motor 602 based on the data transmitted by the laser particle size sensor 402.

[0020] Furthermore, the heat insulation component 7 includes a mounting shell 701, which is fixedly connected to the surface of the extraction box 2. The inner wall of the mounting shell 701 is provided with heat insulation cotton 702, which is in contact with the surface of the extraction box 2. Through the heat insulation component 7, the heat insulation cotton 702 prevents the rapid loss of internal temperature of the extraction box 2, thereby protecting the exosomes inside the extraction box 2.

[0021] Working principle: During use, the separation and extraction component 3 causes the feed box 301 to rotate, which in turn drives multiple separation cores 302 to rotate. The rotation of multiple separation cores 302 generates centrifugal force, which causes the exosomes to pass through the filter holes 303 and filter impurities onto the inner wall of the separation cores 302. This facilitates the filtration and extraction of exosome raw materials and achieves precise separation of impurities and exosomes.

[0022] The monitoring component 4 allows the laser particle size sensor 402 to capture the sedimentation trajectory of exosomes and impurities inside the extraction chamber 2 in real time, thereby adjusting the rotation speed of the extraction chamber 2 according to the sedimentation trajectory and improving the separation efficiency of exosomes.

[0023] The linkage component 5 facilitates the linkage between the separation and extraction component 3 and the monitoring component 4, so that when the feed box 301 rotates, it drives the rotating tube 401 and the laser particle size sensor 402 to rotate synchronously, thereby facilitating the dynamic monitoring of exosomes inside the extraction box 2 by the laser particle size sensor 402 and improving the monitoring accuracy.

[0024] The drive component 6 enables the output end of the fixed frame 601 to rotate, which in turn drives the linkage column 501 to rotate. The rotation of the linkage column 501 drives the separation and extraction component 3 and the monitoring component 4 to rotate synchronously, thereby facilitating the driving and control of the rotation speed of the separation and extraction component 3 and the monitoring component 4. At the same time, the controller 603 enables the laser particle size sensor 402 to transmit the monitored data to the controller 603. The controller 603 then automatically controls the rotation speed of the motor 602 based on the data transmitted by the laser particle size sensor 402.

[0025] The heat insulation component 7 is designed to prevent rapid heat loss inside the extraction box 2 through the heat insulation cotton 702, thereby protecting the exosomes inside the extraction box 2.

[0026] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A multifunctional stem cell exosome extraction device, comprising a mounting frame (1), characterized in that, The mounting bracket (1) is fixedly connected to the inner wall of the extraction box (2), and also includes: Separation and extraction component (3), which is disposed on the inner wall of the mounting frame (1) and is used to separate and extract stem cell exosomes from impurities; Monitoring component (4), the monitoring component (4) is disposed on the inner wall of the monitoring component (4) for real-time monitoring of stem cell exosome raw materials inside the extraction box (2); Linkage component (5), which is disposed on the side wall of the mounting frame (1) and is used to link the separation extraction component (3) and the monitoring component (4); A drive component (6) is disposed on the top surface of the mounting bracket (1) and is used to drive the linkage component (5); The heat preservation component (7) is disposed on the surface of the extraction box (2) and is used to keep the exosomes inside the extraction box (2) warm.

2. The multifunctional stem cell exosome extraction device according to claim 1, characterized in that: The separation and extraction assembly (3) includes a feeding box (301), which is rotatably connected to the inner wall of the extraction box (2). Multiple separation cores (302) are fixedly connected to the inner wall of the feeding box (301). Multiple filter holes (303) are opened on the surface of the separation cores (302). A feeding pipe (304) is fixedly connected to the inner wall of the feeding box (301). The surface of the feeding pipe (304) is rotatably connected to the inner wall of the extraction box (2).

3. The multifunctional stem cell exosome extraction device according to claim 1, characterized in that: The monitoring component (4) includes a rotating tube (401), which is rotatably connected to the inner wall of the extraction box (2). A laser particle size sensor (402) is fixedly connected to the inner wall of the rotating tube (401), and a high-transparency glass protective cover (403) is fixedly connected to the upper end of the rotating tube (401).

4. The multifunctional stem cell exosome extraction device according to claim 3, characterized in that: The linkage component (5) includes a linkage column (501), which is rotatably connected to the side wall of the mounting frame (1). Two first synchronous pulleys (502) are fixedly connected to the surface of the linkage column (501). A third synchronous pulley (503) is fixedly connected to the surface of the rotating tube (401) and the surface of the feed tube (304). The surface of the third synchronous pulley (503) and the surface of the first synchronous pulley (502) are fitted with the same synchronous belt (504).

5. The multifunctional stem cell exosome extraction device according to claim 4, characterized in that: The drive assembly (6) includes a fixed frame (601), which is fixedly connected to the top surface of the mounting frame (1). A motor (602) is fixedly connected to the top surface of the fixed frame (601). The surface of the linkage column (501) is fixedly connected to the output end of the motor (602). A controller (603) is fixedly connected to the top surface of the mounting frame (1). The controller (603) is electrically connected to the motor (602) and to the laser particle size sensor (402).

6. The multifunctional stem cell exosome extraction device according to claim 1, characterized in that: The heat preservation component (7) includes a mounting shell (701), which is fixedly connected to the surface of the extraction box (2). The inner wall of the mounting shell (701) is provided with heat preservation cotton (702), which is attached to the surface of the extraction box (2).

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