Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vaccine antigens of Moraxella

a technology of moraxella and antigens, which is applied in the field of vaccine antigens of moraxella, can solve the problems of limited success of therapeutic and preventive measures in controlling ibk, inability to achieve expression levels of all seven pilus serotypes at levels high enough to be useful for commercial vaccine preparation, and inability to clone the gene encoding the haemolysin gene or purify the protein to homogeneity

Inactive Publication Date: 2006-11-16
FARN JACINTA +2
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therapeutic and preventative measures have limited success in controlling IBK and a vaccine which will prevent the disease is required.
Expression of all seven pilus serotypes at levels high enough to be useful for commercial vaccine preparation has not been achieved.
However, researchers have so far been unable to either clone the gene encoding the haemolysin or purify the protein to homogeneity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vaccine antigens of Moraxella
  • Vaccine antigens of Moraxella
  • Vaccine antigens of Moraxella

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0070] This example describes the cloning and characterisation of a protease from Moraxella bovis.

Bacteria and construction of a genomic library

[0071]Moraxella bovis strain Dalton 2d was a field isolate collected from a bovine eye and characterised by CSIRO Animal Health, Parkville, Australia (6). Escherichia coli strain DH5α has been previously described (7, 8).

[0072] All enzymes were purchased from Promega (Madison, Wis., USA) except where otherwise noted.

[0073] General cloning and DNA techniques were as described (9) unless otherwise noted.

[0074] A genomic library was constructed by carrying out partial Sau3A digests on genomic DNA extracted from M. bovis strain Dalton 2d using a CTAB method which is outlined below. This DNA was size fractionated using a NaCl gradient (10) and ligated with the cosmid cloning vector pHC79 (11) which had been previously digested with BamHI. This DNA was packaged into lambda bacteriophage heads using the Packagene Lambda DNA packaging system (...

example 2

[0093] This example describes the cloning and characterisation of a lipase from Moraxella bovis.

Bacteria and construction of a plasmid library

[0094]Moraxella bovis strain Dalton 2d was a field isolate collected from a bovine eye and characterised by CSIRO Animal Health, Parkville, Australia (6). Escherichia coli strain MC1061 has been previously described (16).

[0095] All enzymes were purchased from Promega (Madison, Wis., USA) except where otherwise noted. General cloning and DNA techniques were as described (9) unless otherwise noted.

[0096] A plasmid library was constructed in the cloning vector pBR322 (17). This was done by partially digesting genomic DNA extracted from Dalton 2d (using the CTAB method described in Example 1) with Sau3A under conditions that maximised the amount of DNA in the range of 1 to 2 kb. This DNA was ligated with pBR322 which had been previously digested with BamHI. The ligated DNA was electroporated (2.5 kV, 200Ω and 200 μF, for a theoretical time co...

example 3

Bacteria and construction of a haemolysin clone

[0123]Moraxella bovis strain Dalton 2d was a field isolate collected from a bovine eye and characterised by CSIRO Animal Health, Parkville, Australia (6).

[0124] All of the M. bovis strains representative of the known pilus serotypes express a haemolytic activity that is detected on horse blood agar.

[0125]Esclierichia coli strain degP4::Tn5 has a leaky outer membrane and is defective in proteolysis and has been previously described (24). All enzymes were purchased from Promega (Madison, Wis., USA) except where otherwise noted.

[0126] General cloning and DNA techniques were as described (9) unless otherwise noted.

[0127] A phoA fusion technique that allows for the identification of exported proteins (25) was utilised with some modifications. Genomic DNA from M. bovis Dalton 2d (prepared using the CTAB method described in Example 1) was partially digested with Sau3A. Restricted DNA was ligated with a series of vectors that allow fusion...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
melting temperatureaaaaaaaaaa
temperatureaaaaaaaaaa
Login to View More

Abstract

The present invention relates to antigens of Moraxella, in particular, Moraxella bovis, nucleic acid sequences encoding these antigens and formulations for use in raising an immune response against Moraxella.

Description

FIELD OF THE INVENTION [0001] The present invention relates to antigens of Moraxella, in particular, Moraxella bovis, nucleic acid sequences encoding these antigens and formulations for use in raising an immune response against Moraxella. BACKGROUND OF THE INVENTION [0002] Infectious bovine keratoconjunctivitis (IBK) is an economically important disease of cattle caused by the Gram-negative coccobacillus Moraxella bovis. More commonly known as pinkeye, IBK is a highly contagious ocular infection which may range from mild conjunctivitis to severe ulceration, corneal perforation and blindness. Therapeutic and preventative measures have limited success in controlling IBK and a vaccine which will prevent the disease is required. A number of factors contribute to the virulence of the organism, the two most important attributes so far identified are the expression of pili, and the ability to produce haemolysin. Seven different serogroups of M. bovis strains isolated in Australia, Great Br...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00A61K39/38C07K14/21C07K16/40C12N9/52C12N15/31
CPCA61K39/00A61K2039/53C12N9/52C07K16/40C07K14/212
Inventor FARN, JACINTASTRUGNELL, RICHARDTENNENT, JAN
Owner FARN JACINTA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com