Deltamethrin antigen and antibody, as well as use thereof

Abstract

The invention discloses a decamethrin antigen, an antibody and application thereof, which belong to the technical field of immune chemical analysis, and provides a method special for preparing decamethrin specific antibody and detecting enzyme coupled immunity. The method comprises the following steps: synthesizing hapten 1-carboxyl-(3'-phenoxyl phenyl) methyl-3-(2', 2'-dibromo vinyl)-2, 2-dimethyl cyclopropyl carboxylic ester (Med for short) and N-2-(carboxyl propyl) carbamoyl-(3'-phenoxyl phenyl) methyl-3-(2', 2'-dibromo vinyl)-2, 2-dimethyl cyclopropyl carboxylic ester (Di for short); preparing artificial immune antigen by coupling the Di and protein; obtaining specific polyclonal antibody of decamethrin by an immune rabbit; preparing envelope antigen by coupling the Med and the protein; and finally establishing an enzyme coupled immunity analytic method by using the synthesized envelope antigen and the specific antibody, wherein the detection limit of the decamethrin reaches 0.004 milligram per liter, the linearity is between 0.005 and 100 milligrams per kilogram, and the decamethrin antigen has no remarkable cross reaction for most pyrethroid and metabolite thereof.

Description

1. Technical field

[0001] The invention relates to a deltamethrin antigen, an antibody and applications thereof, belonging to the technical field of immunochemical analysis, and is specially used for the preparation of deltamethrin-specific antibodies and an enzyme-linked immunoassay method. 2. Background technology

[0002] Deltamethrin is a synthetic pyrethroid insecticide developed by ELLIOT et al. in 1974. Its trade name is Dixid. The chemical name and structure are shown in the figure below. The pure product is a white orthorhombic needle-like crystal, mp: 101~102℃, slightly soluble in water, soluble in most organic solvents, stable under acidic conditions. It has stomach poisoning and contact killing effects, fast knockdown and repelling activity. It is particularly effective against Lepidoptera larvae and is one of the most effective pyrethroid insecticides in the contemporary era.

[0003] At present, the residual analysis of deltamethrin is mainly performed by gas...

Examples

Embodiment Construction

[0039] 1 Synthesis of artificial hapten of deltamethrin

[0040] In order to fully expose the three-dimensional structural characteristics of deltamethrin and contain carboxyl groups that can be coupled with proteins, α-cyano-3-phenoxybenzyl alcohol, deltamethrin and sulfone chloride were used as raw materials to undergo three steps The reaction synthesized the hapten 1-carboxy-(3′-phenoxyphenyl)methyl-3-(2′,2′-dibromovinyl)-2,2-dimethylcyclopropylcarboxylate (Med for short). In order to improve the detection sensitivity through heterologous reaction, the hapten Med was reacted with 4-aminobutyric acid to synthesize N-2-(carboxypropyl)carbamoyl-(3′-phenoxyphenyl)methyl -3-(2',2'-dibromovinyl)-2,2-dimethylcyclopropylcarboxylate (abbreviated as Di). After purification, the products Med and Di were subjected to mass spectrometry (ESI) and H NMR spectroscopy ( 1 H-NMR) identification, and confirmed by comparison with similar known compound data.

[0041] The structure of the h...

1. Technical field

[0001] The invention relates to a deltamethrin antigen, an antibody and applications thereof, belonging to the technical field of immunochemical analysis, and is specially used for the preparation of deltamethrin-specific antibodies and an enzyme-linked immunoassay method. 2. Background technology

[0002] Deltamethrin is a synthetic pyrethroid insecticide developed by ELLIOT et al. in 1974. Its trade name is Dixid. The chemical name and structure are shown in the figure below. The pure product is a white orthorhombic needle-like crystal, mp: 101~102℃, slightly soluble in water, soluble in most organic solvents, stable under acidic conditions. It has stomach poisoning and contact killing effects, fast knockdown and repelling activity. It is particularly effective against Lepidoptera larvae and is one of the most effective pyrethroid insecticides in the contemporary era.

[0003] At present, the residual analysis of deltamethrin is mainly performed by gas...

Examples

Embodiment Construction

[0039] 1 Synthesis of artificial hapten of deltamethrin

[0040] In order to fully expose the three-dimensional structural characteristics of deltamethrin and contain carboxyl groups that can be coupled with proteins, α-cyano-3-phenoxybenzyl alcohol, deltamethrin and sulfone chloride were used as raw materials to undergo three steps The reaction synthesized the hapten 1-carboxy-(3′-phenoxyphenyl)methyl-3-(2′,2′-dibromovinyl)-2,2-dimethylcyclopropylcarboxylate (Med for short). In order to improve the detection sensitivity through heterologous reaction, the hapten Med was reacted with 4-aminobutyric acid to synthesize N-2-(carboxypropyl)carbamoyl-(3′-phenoxyphenyl)methyl -3-(2',2'-dibromovinyl)-2,2-dimethylcyclopropylcarboxylate (abbreviated as Di). After purification, the products Med and Di were subjected to mass spectrometry (ESI) and H NMR spectroscopy ( 1 H-NMR) identification, and confirmed by comparison with similar known compound data.

[0041] The structure of the h...

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