Method for refining pseudo-ginseng root total amino acid by applying membrane separation technology

Abstract

The invention relates to a method for refining pseudo-ginseng root total amino acid by applying a membrane separation technology, in particular to a method for refining pseudo-ginseng root total amino acid by using a microfiltration membrane and a nanofiltration membrane. The method comprises the following steps of: grinding pseudo-ginseng root leaves; adding water and extracting for 1-3 times; combining extracting solutions; adding a flocculating agent under the stirring action; removing precipitates; adding a clarifying solution into a microfiltration device for microfiltration; adding microfiltration fluid into a nanofiltration device for nanofiltration; recycling a solvent from permeate liquid to a small volume; adding ethanol for precipitating; refluxing, dissolving and crystalizing the precipitates with ethanol with the concentration of 50-70 percent for 2-4 times; and drying to obtain a product. The method has the advantages of easiness of operation, no loss of amino acid and easy amplification of process.

Description

Technical field:

[0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for refining notoginseng total amino acid using a microfiltration membrane and a nanofiltration membrane. Background technique:

[0002] Radix Notoginseng, Panax notoginseng (Burk.) F.H.Chen, also known as Radix Notoginseng, Tianqi, Sanqi, Jinbuhuan, Araliaceae Panax genus plant, warm in nature, sweet in taste, slightly bitter. Functions and indications: dissipating blood stasis to stop bleeding, reducing swelling and relieving pain. Used for various internal and external bleeding, tingling pain in the chest and abdomen, swelling and pain from tumbling. "Compendium of Materia Medica" records that Panax notoginseng "resembles the taste of ginseng", "stops bleeding, disperses blood and relieves pain" can treat all blood diseases, and the functions and indications of Panax notoginseng are also defined in previous editions of Chinese Pharmacopoeia ...

Examples

Embodiment 1

[0024] Crush notoginseng leaves, weigh 1Kg, add 12L water to soak and extract twice, filter, combine the extracts, take 2.5g CTS traditional Chinese medicine flocculant, add 250ml water to dilute, add CTS diluent to the extract under rapid stirring, and mix well Then stir at a slow speed, let it stand for 3 hours, filter to remove the precipitate, add the filtrate to the alumina ceramic membrane for microfiltration, the cutoff is 200nm, and the filtration pressure is 200kPa, collect the microfiltrate and add the polyester composite nanofiltration membrane with a molecular cutoff of 400 Medium nanofiltration, the filtration pressure is 500kPa, collect the permeate, recover the solvent to a small volume, add ethanol to precipitate, filter the precipitate and dissolve it with 50% ethanol solution, concentrate to 1 / 3 volume, and stand for crystallization twice , and dried to obtain 8.1 g of white solid, containing 72.2% of notoginseng.

Embodiment 2

[0026] Crush notoginseng leaves, weigh 1Kg, add 5L of water to reflux and extract twice, filter, combine the extract, take 6g of CTS traditional Chinese medicine flocculant, add 300ml of water to dilute, add CTS diluent to the extract under rapid stirring, and mix well Stir slowly, let it stand for 5 hours, centrifuge to remove the precipitate, add the centrifugate into the titanium oxide ceramic membrane for microfiltration, the cutoff is 100nm, and the filtration pressure is 500kPa, collect the microfiltrate and add the polyethylene composite nanofiltration membrane with a molecular cutoff of 300 Medium nanofiltration, the filtration pressure is 600kPa, collect the permeate, recover the solvent to a small volume, add ethanol to precipitate, filter the precipitate and dissolve it with 60% ethanol solution, concentrate to 1 / 4 volume, and stand for crystallization for 3 times , and dried to obtain 7.3 g of white solid, containing 74.9% of notoginseng.

Embodiment 3

[0028] Crush the leaves of Notoginseng, weigh 1Kg, add 6L water to ultrasonically extract twice, filter, combine the extracts, take 4g of CTS traditional Chinese medicine flocculant, add 300ml of water to dilute, add CTS diluent to the extract under rapid stirring, and mix well Stir slowly, let it stand for 6 hours, filter to remove the precipitate, add the filtrate to the zirconia ceramic membrane for microfiltration, the cutoff is 200nm, and the filtration pressure is 300kPa, collect the microfiltrate and add it to the polyamide composite nanofiltration membrane with a molecular cutoff of 200 Nanofiltration, the filtration pressure is 800kPa, the permeate is collected, the solvent is recovered to a small volume, ethanol is added to precipitate, the precipitate is filtered out, heated and dissolved with 65% ethanol solution, concentrated to 1 / 5 volume, and left to crystallize twice, Dry to obtain 8.7 g of white solid, containing 68.4% of notoginseng.

Technical field:

[0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for refining notoginseng total amino acid using a microfiltration membrane and a nanofiltration membrane. Background technique:

[0002] Radix Notoginseng, Panax notoginseng (Burk.) F.H.Chen, also known as Radix Notoginseng, Tianqi, Sanqi, Jinbuhuan, Araliaceae Panax genus plant, warm in nature, sweet in taste, slightly bitter. Functions and indications: dissipating blood stasis to stop bleeding, reducing swelling and relieving pain. Used for various internal and external bleeding, tingling pain in the chest and abdomen, swelling and pain from tumbling. "Compendium of Materia Medica" records that Panax notoginseng "resembles the taste of ginseng", "stops bleeding, disperses blood and relieves pain" can treat all blood diseases, and the functions and indications of Panax notoginseng are also defined in previous editions of Chinese Pharmacopoeia ...

Examples

Embodiment 1

[0024] Crush notoginseng leaves, weigh 1Kg, add 12L water to soak and extract twice, filter, combine the extracts, take 2.5g CTS traditional Chinese medicine flocculant, add 250ml water to dilute, add CTS diluent to the extract under rapid stirring, and mix well Then stir at a slow speed, let it stand for 3 hours, filter to remove the precipitate, add the filtrate to the alumina ceramic membrane for microfiltration, the cutoff is 200nm, and the filtration pressure is 200kPa, collect the microfiltrate and add the polyester composite nanofiltration membrane with a molecular cutoff of 400 Medium nanofiltration, the filtration pressure is 500kPa, collect the permeate, recover the solvent to a small volume, add ethanol to precipitate, filter the precipitate and dissolve it with 50% ethanol solution, concentrate to 1 / 3 volume, and stand for crystallization twice , and dried to obtain 8.1 g of white solid, containing 72.2% of notoginseng.

Embodiment 2

[0026] Crush notoginseng leaves, weigh 1Kg, add 5L of water to reflux and extract twice, filter, combine the extract, take 6g of CTS traditional Chinese medicine flocculant, add 300ml of water to dilute, add CTS diluent to the extract under rapid stirring, and mix well Stir slowly, let it stand for 5 hours, centrifuge to remove the precipitate, add the centrifugate into the titanium oxide ceramic membrane for microfiltration, the cutoff is 100nm, and the filtration pressure is 500kPa, collect the microfiltrate and add the polyethylene composite nanofiltration membrane with a molecular cutoff of 300 Medium nanofiltration, the filtration pressure is 600kPa, collect the permeate, recover the solvent to a small volume, add ethanol to precipitate, filter the precipitate and dissolve it with 60% ethanol solution, concentrate to 1 / 4 volume, and stand for crystallization for 3 times , and dried to obtain 7.3 g of white solid, containing 74.9% of notoginseng.

Embodiment 3

[0028] Crush the leaves of Notoginseng, weigh 1Kg, add 6L water to ultrasonically extract twice, filter, combine the extracts, take 4g of CTS traditional Chinese medicine flocculant, add 300ml of water to dilute, add CTS diluent to the extract under rapid stirring, and mix well Stir slowly, let it stand for 6 hours, filter to remove the precipitate, add the filtrate to the zirconia ceramic membrane for microfiltration, the cutoff is 200nm, and the filtration pressure is 300kPa, collect the microfiltrate and add it to the polyamide composite nanofiltration membrane with a molecular cutoff of 200 Nanofiltration, the filtration pressure is 800kPa, the permeate is collected, the solvent is recovered to a small volume, ethanol is added to precipitate, the precipitate is filtered out, heated and dissolved with 65% ethanol solution, concentrated to 1 / 5 volume, and left to crystallize twice, Dry to obtain 8.7 g of white solid, containing 68.4% of notoginseng.