Judgment method for quickly distinguishing two similar medicinal materials

A kind of medicinal material and fast technology, applied in the direction of material separation, material analysis, measuring device, etc., can solve the problems of incompatibilities, indistinguishable forms, unstable drug efficacy, etc., and achieve the effect of simple method and guaranteed accuracy

CN103033584BInactive Publication Date: 2014-12-03CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
6 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2014-12-03
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a judgment method for quickly distinguishing two similar medicinal materials. The method comprises the following steps: (1), precisely absorbing swertiamarin standard product solution with different volumes, analyzing by using a high performance liquid chromatograph (HPLC), drawing a standard curve and calculating a regression equation; (2), precisely absorbing gentiopicroside standard product solution with different volumes, analyzing by using the HPLC, drawing a standard curve and calculating a regression equation; (3), verifying the regression equations of the swertiamarin and the gentiopicroside respectively; (4), collecting swertia pseudochinensis hara samples; (5), testing the swertia pseudochinensis hara samples to obtain the swertiamarin content and the gentiopicroside content of the swertia pseudochinensis hara samples respectively; and (6), when the swertiamarin content is less than 1 percent and the gentiopicroside content is more than 1 percent, the swertia pseudochinensis hara samples are Swertia mussotii Franch; and when the swertiamarin content is more than 2 percent and the gentiopicroside content is less than 1 percent, the swertia pseudochinensis hara samples are Swertia franchetiana. The method is simple, convenient and quick, and can guarantee the medication correctness.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for judging medicinal materials, in particular to a method for quickly distinguishing two similar medicinal materials. Background technique

[0002] Western Sichuan Swert Sprouts ( Sweiitia mussotii Franch ) is a plant of the Gentianaceae Swertia genus, a unique medicinal material of Tibetan medicine, recorded in the Ministry of Health of the People's Republic of China "Drug Standards Tibetan Medicine Part One" (1992 edition sixth page), its nature and flavor are: sweet, bitter , cool, rough. Functions and indications are: clearing the liver and promoting gallbladder, reducing all fevers. For jaundice hepatitis, viral hepatitis, blood disease. Qinghai Drug Standards (1992 edition, page 17) contained Chinese Swert Sprouts and Swert Sprouts in Western Sichuan ( Sweiitia Franchetiana Smith ), its nature and flavor are: bitter, cold. Functions and indications are: clearing away heat and reducing inflammation, soothin...

Examples

Embodiment Construction

[0018] A method for quickly distinguishing two similar medicinal materials, comprising the following steps:

[0019] (1) Precisely draw 4.0 μl, 8.0 μl, 12.0 μl, 16.0 μl, and 20.0 μl of 0.232 mg / ml swertiamarin standard solution, and then inject samples respectively, using methanol-0.02% phosphoric acid solution as the The mobile phase is a chromatographic column with octadecylsilane bonded silica gel as a filler, and the analysis is carried out under the conditions that the detection wavelength is 240~260nm, and the number of theoretical plates is not less than 3000 based on the peak of swertiamarin, and the determination Integrate the peak area, and finally take the integral value of the peak area as the ordinate, and the swertiamarin content as the abscissa, and draw a standard curve (see figure 1 ), and calculated the regression equation of swertiamarin Y=96470.4x+226293.15, r=0.9998, indicating that swertiamarin has a good linear relationship in the range of 0.93μg~4.64μg...