Method for preparing lauric acid monoglyceride by immobilized lipase

Abstract

The invention discloses a method for preparing monoglyceride laurate by immobilized lipase catalysis. The process steps are as follows: (1) transesterification: mix an appropriate amount of coconut oil and glycerin, and heat to a preset temperature to immobilize lipase As a catalyst for transesterification; (2) centrifugation: the mixture after transesterification is centrifuged, and the upper oil phase is a mixture of fatty acid glycerides; (3) Molecular distillation: the mixture of glycerides is molecularly distilled under certain conditions, After the primary molecular distillation, the steamed residue is taken, and the steamed residue is subjected to the secondary molecular distillation, and the light phase is evaporated, which is the finished product of monoglyceride laurate. The invention has mild reaction conditions, simple operation, high production efficiency, low cost, no harm to the environment, strong catalyst specificity and reusability, and can prepare monoglyceride laurate with higher purity.

Description

technical field

[0001] The invention relates to a method for preparing monoglyceride laurate, in particular to a method for preparing monoglyceride laurate by using immobilized lipase to catalyze the transesterification of coconut oil and glycerin, and belongs to the technical field of surfactant synthesis. Background technique

[0002] Coconut is a monocotyledon perennial evergreen tree of the palm family, and is a typical tropical woody oil crop. About 87% of coconut plantations are located in member countries of the Asia-Pacific Coconut Community (APCC). Among them, the planting areas of Indonesia and the Philippines account for 23.4% and 27.5% of the world's total respectively. In my country, coconuts are mainly produced in the southeast coast of Hainan Province. Coconut oil is the oil extracted from coconut endosperm after crushing and steaming. It is recognized as a vegetable oil with multiple physiological functions in the world. It can not only promote the absorption...

Examples

Embodiment 1

[0024] (1) transesterification

[0025] Add 55.7 grams of coconut oil and 29.1 grams of glycerin into a 150mL flat-bottomed flask (the molar ratio of the two is 1:3, and the water content of glycerin is 4%). After the temperature (50°C), add 4.456g Novozym435 immobilized lipase (Novozymes) (the amount added is 8% of the weight of the above coconut oil), stir constantly, and continue to place it in the above constant temperature water bath for the transesterification reaction. Time 36h.

[0026] (2) centrifugal

[0027] The transesterification product obtained in step (1) is placed in a centrifuge with a centrifugal force of 5000g to separate the immobilized lipase and unreacted glycerin, and the upper oil phase is taken as a mixture of fatty acid glycerides.

[0028] (3) Primary molecular distillation

[0029] The fatty acid glyceride mixture obtained in step (2) is subjected to primary molecular distillation using a scraped-film short-range molecular distillation apparatus...

Embodiment 2

[0033] (1) transesterification

[0034] Add 55.7 grams of coconut oil and 38.8 grams of glycerin into a 150mL flat-bottomed flask (the molar ratio of the two is 1:4, and the water content of glycerin is 5%). After the temperature (40°C), add 3.342g LipozymeTLIM immobilized lipase (same as above) (addition amount is 6% of the weight of the above coconut oil), stir continuously, and continue to place in the above constant temperature water bath for transesterification reaction for 24 hours.

[0035] (2) centrifugal

[0036] The transesterification product obtained in step (1) is placed in a centrifuge with a centrifugal force of 5500g to separate the immobilized lipase and unreacted glycerin, and the upper oil phase is taken as a mixture of fatty acid glycerides.

[0037] (3) Primary molecular distillation

[0038] The fatty acid glyceride mixture obtained in step (2) is subjected to primary molecular distillation using a wiped-film short-range molecular distillation apparatus...

Embodiment 3

[0042] (1) transesterification

[0043] Add 55.7 grams of coconut oil and 38.8 grams of glycerin into a 150mL flat-bottomed flask (the molar ratio of the two is 1:4, and the water content of glycerin is 5%). After the temperature (40°C), add 2.785g LipozymeRMIM immobilized lipase (same as above) (the amount added is 5% of the weight of the above coconut oil), stir constantly, and continue to place it in the above constant temperature water bath for transesterification for 48 hours.

[0044] (2) centrifugal

[0045] The transesterification product obtained in step (1) is placed in a centrifuge with a centrifugal force of 5000g to separate the immobilized lipase and unreacted glycerin, and the upper oil phase is taken as a mixture of fatty acid glycerides.

[0046] (3) Primary molecular distillation

[0047]The fatty acid glyceride mixture obtained in step (2) is subjected to primary molecular distillation using a wiped-film short-range molecular distillation apparatus (same a...

technical field

[0001] The invention relates to a method for preparing monoglyceride laurate, in particular to a method for preparing monoglyceride laurate by using immobilized lipase to catalyze the transesterification of coconut oil and glycerin, and belongs to the technical field of surfactant synthesis. Background technique

[0002] Coconut is a monocotyledon perennial evergreen tree of the palm family, and is a typical tropical woody oil crop. About 87% of coconut plantations are located in member countries of the Asia-Pacific Coconut Community (APCC). Among them, the planting areas of Indonesia and the Philippines account for 23.4% and 27.5% of the world's total respectively. In my country, coconuts are mainly produced in the southeast coast of Hainan Province. Coconut oil is the oil extracted from coconut endosperm after crushing and steaming. It is recognized as a vegetable oil with multiple physiological functions in the world. It can not only promote the absorption...

Examples

Embodiment 1

[0024] (1) transesterification

[0025] Add 55.7 grams of coconut oil and 29.1 grams of glycerin into a 150mL flat-bottomed flask (the molar ratio of the two is 1:3, and the water content of glycerin is 4%). After the temperature (50°C), add 4.456g Novozym435 immobilized lipase (Novozymes) (the amount added is 8% of the weight of the above coconut oil), stir constantly, and continue to place it in the above constant temperature water bath for the transesterification reaction. Time 36h.

[0026] (2) centrifugal

[0027] The transesterification product obtained in step (1) is placed in a centrifuge with a centrifugal force of 5000g to separate the immobilized lipase and unreacted glycerin, and the upper oil phase is taken as a mixture of fatty acid glycerides.

[0028] (3) Primary molecular distillation

[0029] The fatty acid glyceride mixture obtained in step (2) is subjected to primary molecular distillation using a scraped-film short-range molecular distillation apparatus...

Embodiment 2

[0033] (1) transesterification

[0034] Add 55.7 grams of coconut oil and 38.8 grams of glycerin into a 150mL flat-bottomed flask (the molar ratio of the two is 1:4, and the water content of glycerin is 5%). After the temperature (40°C), add 3.342g LipozymeTLIM immobilized lipase (same as above) (addition amount is 6% of the weight of the above coconut oil), stir continuously, and continue to place in the above constant temperature water bath for transesterification reaction for 24 hours.

[0035] (2) centrifugal

[0036] The transesterification product obtained in step (1) is placed in a centrifuge with a centrifugal force of 5500g to separate the immobilized lipase and unreacted glycerin, and the upper oil phase is taken as a mixture of fatty acid glycerides.

[0037] (3) Primary molecular distillation

[0038] The fatty acid glyceride mixture obtained in step (2) is subjected to primary molecular distillation using a wiped-film short-range molecular distillation apparatus...

Embodiment 3

[0042] (1) transesterification

[0043] Add 55.7 grams of coconut oil and 38.8 grams of glycerin into a 150mL flat-bottomed flask (the molar ratio of the two is 1:4, and the water content of glycerin is 5%). After the temperature (40°C), add 2.785g LipozymeRMIM immobilized lipase (same as above) (the amount added is 5% of the weight of the above coconut oil), stir constantly, and continue to place it in the above constant temperature water bath for transesterification for 48 hours.

[0044] (2) centrifugal

[0045] The transesterification product obtained in step (1) is placed in a centrifuge with a centrifugal force of 5000g to separate the immobilized lipase and unreacted glycerin, and the upper oil phase is taken as a mixture of fatty acid glycerides.

[0046] (3) Primary molecular distillation

[0047]The fatty acid glyceride mixture obtained in step (2) is subjected to primary molecular distillation using a wiped-film short-range molecular distillation apparatus (same a...

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