A kind of urine calcium ion detection test paper and preparation method thereof

Abstract

The invention discloses urine calcium ion detection test paper and a method for preparing the urine calcium ion detection test paper. The detection test paper comprises a base plate and a filter paper sheet which are fixedly connected, and the filter paper sheet is made by fully immersing filter paper in liquid, taking out the filter paper and drying the filter paper. The liquid A is water solution comprising 1-1.5wt% of buffering liquid, 0.35-0.58wt% of o-cresolphthalein complexon, 10-12.5wt% of surface active agents, 0.35-0.55wt% of masking agents and 0.03-0.05wt% of lemon yellow. Under alkaline conditions, when calcium ion concentration ranges from 0 to 10mmol / L, chromatic indexes from light yellow to dark red appear, and therefore visual recognition is facilitated. The test paper is easy in preparation method, low in preparing cost, easy to operate and stable in performance, and the detection results can be quickly and accurately acquired.

Description

technical field

[0001] The invention relates to the field of urine detection, in particular to a urine calcium ion detection test paper and a preparation method thereof. Background technique

[0002] Calcium is one of the essential elements for the human body and one of the most abundant elements in the human body, and is closely related to various physiological activities. The change of urinary calcium can reflect the change of blood calcium, and the abnormality (increase or decrease) of urinary calcium is related to many diseases, such as hypercalcemia, hyperparathyroidism, multiple myeloma, bone malignancy and so on. Therefore, the determination of urinary calcium is of great significance.

[0003] At present, the commonly used methods for calcium determination include complexometric titration and atomic absorption method. These methods require relevant instruments and equipment and specially trained testing personnel. The operation is complicated and the detection speed...

Examples

Embodiment 1

[0022] The present embodiment provides a urine calcium ion detection test paper, which is prepared through the following steps:

[0023] (1) Prepare liquid A: put 12.5g of 2-amino-2-methyl-1-propanol, 4.6g of o-cresolphthalein complex ketone, 113g of polyvinylpyrrolidone, 4.5g of 8-hydroxyquinoline, and 0.4g of tartrazine into In the volumetric flask, add water to 1000mL; after testing with pH test paper, the pH of liquid A=11;

[0024] (2) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 70°C to dry for 15 minutes;

[0025] (3) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine calcium ion detection test paper.

Embodiment 2

[0027] The present embodiment provides a urine calcium ion detection test paper, which can also be prepared through the following steps:

[0028] (1) Configuration A liquid: 12g 2-amino-2-methyl-1,3-propanediol, 5.5g o-cresolphthalein complex ketone, 95g polyvinylpyrrolidone, 20g polyethylene oxide laurate ether, 4.8g 8-hydroxy Put quinoline and 0.4g tartrazine into a volumetric flask, add water to 1000mL; test with pH test paper, the pH of liquid A=11;

[0029] (2) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 60°C to dry for 15 minutes;

[0030] (3) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine calcium ion detection test paper.

Embodiment 3

[0032] Preparation method of standard color card:

[0033] (1) Prepare calcium ion standard solutions of 0mmol / L, 2.5mmol / L, 5.0mmol / L, 7.5mmol / L, 10mmol / L;

[0034] (2) The detection test paper prepared by embodiment 1 or embodiment 2 is immersed in the above-mentioned five kinds of calcium ion standard solutions respectively, takes out after 2 seconds, dries, and develops color completely after 20 seconds;

[0035] (3) Prepare a color comparison card according to the color development of each test paper, and the color scale of the color comparison card is 5 color levels from light yellow to dark red.

technical field

[0001] The invention relates to the field of urine detection, in particular to a urine calcium ion detection test paper and a preparation method thereof. Background technique

[0002] Calcium is one of the essential elements for the human body and one of the most abundant elements in the human body, and is closely related to various physiological activities. The change of urinary calcium can reflect the change of blood calcium, and the abnormality (increase or decrease) of urinary calcium is related to many diseases, such as hypercalcemia, hyperparathyroidism, multiple myeloma, bone malignancy and so on. Therefore, the determination of urinary calcium is of great significance.

[0003] At present, the commonly used methods for calcium determination include complexometric titration and atomic absorption method. These methods require relevant instruments and equipment and specially trained testing personnel. The operation is complicated and the detection speed...

Examples

Embodiment 1

[0022] The present embodiment provides a urine calcium ion detection test paper, which is prepared through the following steps:

[0023] (1) Prepare liquid A: put 12.5g of 2-amino-2-methyl-1-propanol, 4.6g of o-cresolphthalein complex ketone, 113g of polyvinylpyrrolidone, 4.5g of 8-hydroxyquinoline, and 0.4g of tartrazine into In the volumetric flask, add water to 1000mL; after testing with pH test paper, the pH of liquid A=11;

[0024] (2) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 70°C to dry for 15 minutes;

[0025] (3) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine calcium ion detection test paper.

Embodiment 2

[0027] The present embodiment provides a urine calcium ion detection test paper, which can also be prepared through the following steps:

[0028] (1) Configuration A liquid: 12g 2-amino-2-methyl-1,3-propanediol, 5.5g o-cresolphthalein complex ketone, 95g polyvinylpyrrolidone, 20g polyethylene oxide laurate ether, 4.8g 8-hydroxy Put quinoline and 0.4g tartrazine into a volumetric flask, add water to 1000mL; test with pH test paper, the pH of liquid A=11;

[0029] (2) Immerse the filter paper piece in the liquid A, take it out after fully soaking, and place it in a vacuum drying oven at a temperature of 60°C to dry for 15 minutes;

[0030] (3) Fix the dried filter paper with the substrate, cut into rectangular paper strips, and make a urine calcium ion detection test paper.

Embodiment 3

[0032] Preparation method of standard color card:

[0033] (1) Prepare calcium ion standard solutions of 0mmol / L, 2.5mmol / L, 5.0mmol / L, 7.5mmol / L, 10mmol / L;

[0034] (2) The detection test paper prepared by embodiment 1 or embodiment 2 is immersed in the above-mentioned five kinds of calcium ion standard solutions respectively, takes out after 2 seconds, dries, and develops color completely after 20 seconds;

[0035] (3) Prepare a color comparison card according to the color development of each test paper, and the color scale of the color comparison card is 5 color levels from light yellow to dark red.