Process for extracting curcumin through ultrasonic-aqueous two-phase synergy

Abstract

The invention relates to a process for extracting curcumin through an ultrasonic-aqueous two-phase synergy. The process comprises the following steps of pulverizing curcuma, degreasing, carrying out ultrasonic extraction on curcumin with 75% ethanol to obtain an extract, adding an aqueous solution of ammonium sulfate into the extract, uniformly stirring, standing until an alcohol phase is separated from an aqueous phase in a balanced way, separating the alcohol phase, drying the alcohol phase at 45-50 DEG C to constant weight, dissolving the dried product in 75% ethanol to obtain a solution, enabling the solution to pass through macroporous resin, eluting with 75% ethanol, collecting and drying to obtain preliminarily purified curcumin. According to the process disclosed by the invention, by combining the ultrasonic extraction technology and aqueous two-phase extraction technology and adopting the extraction principle that effective substances are fully dissolved in an organic solvent, the extraction rate is greatly increased, on the basis that respective advantages of the ultrasonic extraction technology and the aqueous two-phase extraction technology are given into a play, the mutual synergistic effect of the ultrasonic extraction technology and the aqueous two-phase extraction technology is achieved and thus the extraction rate of curcumin reaches 18%.

Description

technical field

[0001] The invention relates to the technical field of plant pigment extraction research, in particular to a pure natural curcumin ultrasonic-two-phase synergistic extraction process. Background technique

[0002] Turmeric is a natural food additive approved by WHO and the U.S. Food and Drug Administration (FDA), belonging to the genus Curcuma longa. Curcumin, a natural medicinal and edible substance extracted from turmeric, is favored by users at home and abroad because it is safe and non-toxic and can regulate human physiological functions. The active ingredients that can be extracted from plant turmeric are curcumin and turmeric oil. Currently , The extraction methods of curcumin include alkaline water extraction, enzymatic extraction, alcohol extraction and so on. Alkaline water extraction is low in cost and can obtain pigment products with high purity and can be dried. However, due to the use of strong alkali in the operation process, the reaction condi...

Examples

Embodiment 1

[0020] Taking 10g of raw turmeric as an example, the ultrasonic-two-phase synergistic extraction process is used to extract curcumin in turmeric, which is realized by the following steps:

[0021] (1) take by weighing 10g turmeric, add 200mL excessive sherwood oil after pulverizing with FZ102 type plant pulverizer and be used for deoiling, obtain the turmeric filter cake after degreasing;

[0022] (2) The turmeric filter cake is placed in 100mL concentration of 75% ethanol, the solid-liquid ratio is 1:10, the ultrasonic time is 18min, and the ultrasonic power density is 2.3W / cm 2 , under the condition that the ultrasonic frequency is 23kHz, and the ultrasonic radiation mode is 7s / 10s, the curcumin is extracted by ultrasonic, and the extract is obtained by ultrasonic 4 times;

[0023] (3) the ammonium sulfate of 60g is dissolved in 100mL water, obtains ammonium sulfate aqueous solution, in step (2) gained extract, add the ammonium sulfate aqueous solution of equivalent, make th...

Embodiment 2

[0029] Taking 10g of raw turmeric as an example, the ultrasonic-two-phase synergistic extraction process is used to extract curcumin in turmeric, which is realized by the following steps:

[0030] (1) take by weighing 10g turmeric, add excessive sherwood oil after pulverizing with pulverizer and be used for deoiling, obtain the turmeric filter cake after degreasing;

[0031] (2) The turmeric filter cake is placed in 75% ethanol, the solid-liquid ratio is 1:9, the ultrasonic time is 15min, and the ultrasonic power density is 2.2W / cm 2 , under the condition that the ultrasonic frequency is 20kHz, and the ultrasonic radiation mode is 6s / 10s, the curcumin is extracted by ultrasonic, and the extract is obtained by ultrasonic 3 times;

[0032] (3) the ammonium sulfate of 58g is dissolved in 100mL water, obtains ammonium sulfate aqueous solution, in step (2) gained extract, add the ammonium sulfate aqueous solution of equivalent, make the volume ratio of extract liquid and ammonium s...

Embodiment 3

[0035] Taking 10g of raw turmeric as an example, the ultrasonic-two-phase synergistic extraction process is used to extract curcumin in turmeric, which is realized by the following steps:

[0036] (1) take by weighing 10g turmeric, add excessive sherwood oil after pulverizing with pulverizer and be used for deoiling, obtain the turmeric filter cake after degreasing;

[0037] (2) The turmeric filter cake is placed in 75% ethanol, the solid-liquid ratio is 1:11, the ultrasonic time is 15min, and the ultrasonic power density is 2.2W / cm 2 , under the condition that the ultrasonic frequency is 20kHz, and the ultrasonic radiation mode is 6s / 10s, the curcumin is extracted by ultrasonic, and the extract is obtained by ultrasonic 3 times;

[0038] (3) the ammonium sulfate of 61g is dissolved in 100mL water, obtains the ammonium sulfate aqueous solution, adds the equivalent ammonium sulfate aqueous solution in step (2) gained extract, makes the volume ratio of extract and ammonium sulfa...

technical field

[0001] The invention relates to the technical field of plant pigment extraction research, in particular to a pure natural curcumin ultrasonic-two-phase synergistic extraction process. Background technique

[0002] Turmeric is a natural food additive approved by WHO and the U.S. Food and Drug Administration (FDA), belonging to the genus Curcuma longa. Curcumin, a natural medicinal and edible substance extracted from turmeric, is favored by users at home and abroad because it is safe and non-toxic and can regulate human physiological functions. The active ingredients that can be extracted from plant turmeric are curcumin and turmeric oil. Currently , The extraction methods of curcumin include alkaline water extraction, enzymatic extraction, alcohol extraction and so on. Alkaline water extraction is low in cost and can obtain pigment products with high purity and can be dried. However, due to the use of strong alkali in the operation process, the reaction condi...

Examples

Embodiment 1

[0020] Taking 10g of raw turmeric as an example, the ultrasonic-two-phase synergistic extraction process is used to extract curcumin in turmeric, which is realized by the following steps:

[0021] (1) take by weighing 10g turmeric, add 200mL excessive sherwood oil after pulverizing with FZ102 type plant pulverizer and be used for deoiling, obtain the turmeric filter cake after degreasing;

[0022] (2) The turmeric filter cake is placed in 100mL concentration of 75% ethanol, the solid-liquid ratio is 1:10, the ultrasonic time is 18min, and the ultrasonic power density is 2.3W / cm 2 , under the condition that the ultrasonic frequency is 23kHz, and the ultrasonic radiation mode is 7s / 10s, the curcumin is extracted by ultrasonic, and the extract is obtained by ultrasonic 4 times;

[0023] (3) the ammonium sulfate of 60g is dissolved in 100mL water, obtains ammonium sulfate aqueous solution, in step (2) gained extract, add the ammonium sulfate aqueous solution of equivalent, make th...

Embodiment 2

[0029] Taking 10g of raw turmeric as an example, the ultrasonic-two-phase synergistic extraction process is used to extract curcumin in turmeric, which is realized by the following steps:

[0030] (1) take by weighing 10g turmeric, add excessive sherwood oil after pulverizing with pulverizer and be used for deoiling, obtain the turmeric filter cake after degreasing;

[0031] (2) The turmeric filter cake is placed in 75% ethanol, the solid-liquid ratio is 1:9, the ultrasonic time is 15min, and the ultrasonic power density is 2.2W / cm 2 , under the condition that the ultrasonic frequency is 20kHz, and the ultrasonic radiation mode is 6s / 10s, the curcumin is extracted by ultrasonic, and the extract is obtained by ultrasonic 3 times;

[0032] (3) the ammonium sulfate of 58g is dissolved in 100mL water, obtains ammonium sulfate aqueous solution, in step (2) gained extract, add the ammonium sulfate aqueous solution of equivalent, make the volume ratio of extract liquid and ammonium s...

Embodiment 3

[0035] Taking 10g of raw turmeric as an example, the ultrasonic-two-phase synergistic extraction process is used to extract curcumin in turmeric, which is realized by the following steps:

[0036] (1) take by weighing 10g turmeric, add excessive sherwood oil after pulverizing with pulverizer and be used for deoiling, obtain the turmeric filter cake after degreasing;

[0037] (2) The turmeric filter cake is placed in 75% ethanol, the solid-liquid ratio is 1:11, the ultrasonic time is 15min, and the ultrasonic power density is 2.2W / cm 2 , under the condition that the ultrasonic frequency is 20kHz, and the ultrasonic radiation mode is 6s / 10s, the curcumin is extracted by ultrasonic, and the extract is obtained by ultrasonic 3 times;

[0038] (3) the ammonium sulfate of 61g is dissolved in 100mL water, obtains the ammonium sulfate aqueous solution, adds the equivalent ammonium sulfate aqueous solution in step (2) gained extract, makes the volume ratio of extract and ammonium sulfa...

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