A device for automatically measuring the respiration rate of microorganisms

Abstract

The present invention provides an automatic determination device for microbial respiration rate, which relates to the field of instruments for agricultural and ecological environmental science research. It is used to solve the problem that the prior art cannot automatically and continuously determine the microbial respiration rate in large quantities. It includes: at least one culture bottle; a multi-channel control system, including at least one channel, the channel is correspondingly connected to the culture bottle, and the channel is used to obtain the measured gas in the culture bottle; a carbon dioxide analyzer, connected to the multi-channel control system, and used to measure the carbon dioxide concentration data in the measured gas; a data collector, connected to the carbon dioxide analyzer, and used to collect the carbon dioxide concentration data, and determine the microbial respiration rate according to the carbon dioxide concentration data.

Description

Technical Field

[0001] The invention relates to the field of instruments for agricultural and ecological environmental scientific research, and in particular to an automatic determination device for microbial respiration rate. Background Art

[0002] Soil microbial respiration rate data is an important indicator reflecting the decomposition process of soil organic carbon and microbial activity, and plays an important role in ecosystem ecology research and global carbon cycle. In traditional studies, the methods for obtaining soil microbial respiration rate data are all based on manual measurement methods such as alkali absorption method, infrared analyzer method or gas chromatography, which require a lot of manpower and material resources when the sample volume is large. An automatic measurement device based on multiple sample automatic switching technology will be able to solve these problems well. Although relevant equipment is currently available, it can only achieve automatic switching between 16 samples at most. In this way, when the sample volume is relatively large, it is still necessary to frequently change samples to complete the measurement, which is not only laborious, but also the environmental factors will cause great disturbance to the sample due to manual switching, affecting the validity and accuracy of the measurement results. In addition, the price of related equipment is very expensive, which further limits its wide application. Therefore, it is very urgent to further develop low-cost equipment that can realize automatic measurement of more samples. Summary of the invention

[0003] The embodiment of the present invention provides an automatic microbial respiration rate measuring device, which is used to solve the problem that the prior art cannot continuously measure the microbial respiration rate in large quantities.

[0004] The embodiment of the present invention provides a device for automatically measuring the respiration rate of microorganisms, comprising:

[0005] At least one culture flask;

[0006] A multi-channel control system, comprising at least one channel, wherein the channel is correspondingly connected to the culture bottle, and the channel is used to obtain the measured gas in the culture bottle;

[0007] A carbon dioxide analyzer, connected to the multi-channel control system, for measuring carbon dioxide concentration data in the measured gas;

[0008] A data collector is connected to the carbon dioxide analyzer and is used to collect the carbon dioxide concentration data and determine the microbial respiration rate based on the carbon dioxide concentration data.

[0009] Preferably, the multi-channel control system comprises:

[0010] An air intake solenoid valve group, comprising at least one air intake solenoid valve; the air intake solenoid valve is connected to the culture bottle correspondingly, and is connected to the carbon dioxide analyzer through a first connecting component, and is used to transport the measured gas from the culture bottle to the carbon dioxide analyzer;

[0011] An outlet solenoid valve group, comprising at least one outlet solenoid valve; the outlet solenoid valve is connected to the culture bottle correspondingly, and is connected to the carbon dioxide analyzer through a second connecting component, and is used to transport the measured gas from the carbon dioxide analyzer back to the culture bottle; and is connected to one end of the first air pump through a third connecting component, and is used to transport the atmosphere to the culture bottle to perform a ventilation operation;

[0012] At least one multi-channel time relay, electrically connected to the air intake solenoid valve and the air outlet solenoid valve, respectively, for controlling the working sequence of the air intake solenoid valve and the air outlet solenoid valve;

[0013] Wherein, the air inlet solenoid valve and the air outlet solenoid valve connected to the same culture bottle are interconnected through the carbon dioxide analyzer.

[0014] Preferably, the first connecting component comprises an air outlet and at least one air inlet;

[0015] The second connecting member includes an air inlet and at least one air outlet;

[0016] The third connecting component includes an air inlet and at least one air outlet.

[0017] Preferably, the multi-channel control system further comprises a first filter, one end of the first filter is connected to the other end of the first air pump, and the other end of the first filter is directly connected to the atmosphere.

[0018] Preferably, the multi-channel control system further comprises a second air pump, a second filter and an air guide tube;

[0019] The carbon dioxide analyzer is connected to the air inlet solenoid valve group and the air outlet solenoid valve group through the air guide pipe, and the culture bottle is connected to the air inlet solenoid valve and the air outlet solenoid valve through the air guide pipe;

[0020] The third connecting component is connected to the air outlet solenoid valve group and the first air pump respectively through the air guide pipe;

[0021] The second air pump is arranged between the air intake solenoid valve group and the carbon dioxide analyzer;

[0022] The second filter is disposed between the second air pump and the carbon dioxide analyzer.

[0023] Preferably, it also includes a water vapor saturated gas generating device; the water vapor saturated gas generating device includes: an artificial climate box, a copper particle bottle and a buffer bottle;

[0024] The copper particle bottle and the buffer bottle are located in the artificial climate chamber; the copper particle bottle is connected to the first air pump and the buffer bottle respectively; the buffer bottle is also connected to the third connecting component; the water vapor saturation gas generating device is used to adjust the water vapor concentration of the atmosphere to a saturated state.

[0025] Preferably, the culture bottle is located in the artificial climate box; the artificial climate box is also used to change the temperature of the culture bottle, and determine the temperature sensitivity of microbial respiration by measuring the microbial respiration rate at different temperatures.

[0026] Preferably, a T-shaped three-way component is provided in the culture bottle, which is connected to the multi-channel control system and is used to adjust the flow direction of the measured gas to a horizontal direction.

[0027] An embodiment of the present invention provides an automatic microbial respiration rate determination device, comprising: at least one culture bottle; a multi-channel control system, comprising at least one channel, the channel being connected to the culture bottle correspondingly, the channel being used to obtain the measured gas in the culture bottle; a carbon dioxide analyzer, connected to the multi-channel control system, and used to determine the carbon dioxide concentration data in the measured gas; a data collector, connected to the carbon dioxide analyzer, and used to collect the carbon dioxide concentration data, and determine the microbial respiration rate based on the carbon dioxide concentration data. In the embodiment of the present invention, when a large number of soil samples are continuously and automatically measured, the large number of soil samples are respectively placed in culture bottles, and the channels of the multi-channel control system are connected to each culture bottle one by one, so as to extract the measured gas in each culture bottle in turn and transport the measured gas to the carbon dioxide analyzer, and then transport the measured gas back to the original culture bottle; the carbon dioxide analyzer obtains carbon dioxide concentration data based on the measured gas, and sends the obtained carbon dioxide concentration data to the data collector, so that the data collector determines the microbial respiration rate based on the carbon dioxide concentration data; in this way, the embodiment of the present invention can realize continuous measurement of a large number of soil samples by expanding the number of channels of the control system, and there is no need to manually replace the next batch of culture bottles after measuring a batch of culture bottles, thereby reducing the interference of obtaining the microbial respiration rate due to manual switching, and improving the efficiency and accuracy of the microbial respiration rate measurement. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 A schematic diagram of the structure of a device for automatically measuring the respiration rate of microorganisms provided in an embodiment of the present invention;

[0030] Figure 2 A schematic structural diagram of a second connecting component or a third connecting component provided by an embodiment of the present invention;

[0031] Figure 3 A schematic structural diagram of a water vapor saturated gas generating device provided in an embodiment of the present invention. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Figure 1 The schematic diagram of the structure of the automatic determination device of microbial respiration rate provided by the embodiment of the present invention comprises:

[0034] At least one culture flask.

[0035] A multi-channel control system comprises at least one channel, wherein the channel is correspondingly connected to a culture bottle and is used to obtain the measured gas in the culture bottle.

[0036] The carbon dioxide analyzer is connected to the multi-channel control system and is used to measure the carbon dioxide concentration data in the measured gas.

[0037] The data collector is connected to the carbon dioxide analyzer and is used to collect carbon dioxide concentration data and determine the microbial respiration rate based on the carbon dioxide concentration data.

[0038] Therein, soil samples are placed in respective culture bottles, and the culture bottles are placed in a constant temperature environment; the temperature of the constant temperature environment is determined by the measuring personnel.

[0039] In practical applications, the number of channels of the multi-channel control system can be expanded according to the number of soil samples that need to be measured, so that each culture bottle is connected to each channel of the multi-channel control system one by one; in this way, the multi-channel control system extracts the measured gas in each culture bottle in turn, and then sends the extracted measured gas to the carbon dioxide analyzer to obtain the carbon dioxide concentration data of the measured gas at different times, and at the same time, the measured gas is transported back to the original culture bottle; after the carbon dioxide analyzer obtains the carbon dioxide concentration data at different times, it sends the carbon dioxide concentration data at different times to the data collector; the data collector receives the carbon dioxide concentration data at different times, and determines the microbial respiration rate according to the change of carbon dioxide concentration within a certain period of time.

[0040] Preferably, the multi-channel control system comprises:

[0041] The air intake solenoid valve group includes at least one air intake solenoid valve; the air intake solenoid valve is connected to the culture bottle accordingly, and is connected to the carbon dioxide analyzer through a first connecting component, and is used to transport the measured gas from the culture bottle to the carbon dioxide analyzer.

[0042] The gas outlet solenoid valve group includes at least one gas outlet solenoid valve; the gas outlet solenoid valve is connected to the culture bottle accordingly, and is connected to the carbon dioxide analyzer through a second connecting component, so as to transport the measured gas from the carbon dioxide analyzer back to the culture bottle; and is connected to one end of the first air pump through a third connecting component, so as to transport the atmospheric air to the culture bottle to perform a ventilation operation.

[0043] At least one multi-channel time relay is electrically connected to the air intake solenoid valve and the air outlet solenoid valve respectively, and is used to control the working sequence of the air intake solenoid valve and the air outlet solenoid valve.

[0044] Among them, the air inlet solenoid valve and the air outlet solenoid valve connected to the same culture bottle are interconnected through the carbon dioxide analyzer.

[0045] The number of the air inlet solenoid valves, the number of the air outlet solenoid valves and the number of the culture bottles are the same.

[0046] The carbon dioxide analyzer comprises an air inlet and an air outlet. The air inlet of the carbon dioxide analyzer is connected to the air inlet solenoid valve group, and the air outlet of the carbon dioxide analyzer is connected to the air outlet solenoid valve group.

[0047] The air inlet solenoid valve comprises an air inlet m1, an air outlet m2 and a ventilation port m3; the air inlet m1 is connected to the culture bottle, the air outlet m2 is connected to the air inlet of the carbon dioxide analyzer through a first connecting component, and the ventilation port m3 is directly connected to the atmosphere.

[0048] When the air inlet solenoid valve is powered on, the air inlet m1 and the air outlet m2 of the air inlet solenoid valve are connected, and the microbial respiration rate of the culture bottle connected to the air inlet solenoid valve is measured. When the air inlet solenoid valve is powered off, the air inlet m1 and the ventilation port m3 of the air inlet solenoid valve are connected, and the culture bottle connected to the air inlet solenoid valve is ventilated.

[0049] The air outlet solenoid valve includes an air inlet n1, an air outlet n2 and a ventilation port n3; the air inlet n1 is connected to the air outlet of the carbon dioxide analyzer, the air outlet n2 is connected to the culture bottle through a second connecting component, and the ventilation port n3 is connected to one end of the first air pump through a third connecting component.

[0050] When the outlet solenoid valve is powered on, the air inlet n1 and the air outlet n2 of the outlet solenoid valve are connected, and the microbial respiration rate of the culture bottle connected to the outlet solenoid valve is measured. When the outlet solenoid valve is powered off, the air outlet n2 and the ventilation port n3 of the outlet solenoid valve are connected, and the culture bottle connected to the outlet solenoid valve is ventilated.

[0051] In this way, the gas to be tested in the culture bottle is extracted from the air inlet m1 and the air outlet m2 of the air inlet solenoid valve, and after being measured by the carbon dioxide analyzer, it returns to the original culture bottle through the air inlet n1 and the air outlet n2 of the air outlet solenoid valve.

[0052] It can be understood that the air inlet m1 and the air outlet m2 of the air inlet solenoid valve connected to the same culture bottle, and the air inlet n1 and the air outlet n2 of the air outlet solenoid valve form a channel in the multi-channel control system; wherein the air inlet solenoid valve and the air outlet solenoid valve connected to different culture bottles are not intercommunication. Among them, since the increase in carbon dioxide concentration in a closed environment will inhibit microbial respiration, therefore, in order to improve the accuracy of measuring the microbial respiration rate, when the outlet solenoid valve is powered off, the air outlet n2 and the ventilation port n3 are connected, and when the air inlet solenoid valve is powered off, the air inlet m1 and the ventilation port m3 are connected, so that continuous ventilation of the corresponding culture bottle can be achieved. Since the measurement of the microbial respiration rate occurs when the solenoid valve (including the air inlet solenoid valve and the air outlet solenoid valve) is powered on, and ventilation occurs when the solenoid valve is powered off, the measurement of microbial respiration and ventilation do not interfere with each other. At the same time, only one culture bottle is measuring microbial respiration, and all other culture bottles are performing ventilation operations.

[0053] Based on this, in order to facilitate the control of channel work of multi-channel control systems.

[0054] A multi-channel time relay can be connected to a preset number of channels of a multi-channel control system to control the working order of the channels of the multi-channel control system; the embodiment of the present invention controls the operation of each channel of the multi-channel system through the linkage of at least one multi-channel time relay. For example, the automatic determination device for the respiration rate of microorganisms provided by the present invention includes 7 multi-channel time relays, and one multi-channel time relay includes 16 channels; in this way, the automatic determination of the respiration rate of microorganisms of (7*16=112) culture bottles can be realized.

[0055] Specifically, the multi-channel time relay performs ventilation on the culture bottle and measures the respiration rate of the microorganism by setting the sequence of the air inlet solenoid valves and the sequence of the air outlet solenoid valves.

[0056] The order of the air inlet solenoid valve and the air outlet solenoid valve connected to the same culture bottle is the same.

[0057] The specific implementation method of controlling the operation of the air inlet solenoid valve and the air outlet solenoid valve by a multi-channel time relay includes the following steps:

[0058] S101: The multi-channel time relay supplies power to the inlet solenoid valve 1 and the outlet solenoid valve 1 at the same time, and performs microbial respiration measurement on the culture bottle 1, that is, the measured gas in the culture bottle 1 is drawn into the carbon dioxide analyzer through the inlet solenoid valve 1, and then returns to the culture bottle 1 through the outlet solenoid valve 1; at the same time, all culture bottles except the culture bottle 1 perform the ventilation operation. After one measurement cycle, the inlet solenoid valve 1 and the outlet solenoid valve 1 are de-energized.

[0059] Among them, the air inlet solenoid valve i and the air outlet solenoid valve i are respectively connected to the i-th culture bottle; i is greater than or equal to 1, and i is a positive integer.

[0060] S102: After the inlet solenoid valve 1 and the outlet solenoid valve 1 are powered off, the multi-channel time relay supplies power to the inlet solenoid valve 2 and the outlet solenoid valve 2 at the same time, and the microbial respiration test is performed on the culture bottle 2, that is, the measured gas in the culture bottle 2 is pumped into the carbon dioxide analyzer through the inlet solenoid valve 2, and then returns to the culture bottle 2 through the outlet solenoid valve 2; at the same time, all cultures except the culture bottle 2 perform the ventilation operation. This process is repeated until the microbial respiration test of all culture bottles is completed.

[0061] After all the culture bottles are measured, the working cycle and start time of the multi-channel time relay can be set to automatically start the next cycle of measurement or set the start time of the next cycle as needed.

[0062] Preferably, the first connecting component includes an air outlet and at least one air inlet; the second connecting component includes an air inlet and at least one air outlet; and the third connecting component includes an air inlet and at least one air outlet.

[0063] Among them, the first connecting component includes an air outlet and at least one air inlet; the air outlet of the first connecting component is connected to the carbon dioxide analyzer, and the air inlet of the first connecting component is correspondingly connected to the air outlet m2 of the air intake solenoid valve.

[0064] The second connecting component includes an air inlet and at least one air outlet; the air inlet of the second connecting component is connected to the carbon dioxide analyzer, and the air outlet of the second connecting component is correspondingly connected to the air inlet n1 of the air outlet solenoid valve.

[0065] The third connecting component includes an air inlet and at least one air outlet; the air inlet of the third connecting component is connected to one end of the first air pump, and the air outlet of the third connecting component is correspondingly connected to the air exchange port n3 of the air outlet solenoid valve.

[0066] Among them, the error caused by the memory effect of the last culture bottle measured can be reduced by reducing the volume of the first connecting component and the volume of the second connecting component, that is, the influence of the measured gas remaining in the last culture bottle in the multi-channel control system on the culture bottle being measured at this time, thereby improving the accuracy of measuring the microbial respiration rate.

[0067] In practical applications, the first connecting component, the second connecting component and the third connecting component are all porous devices, which include at least one air inlet and at least one air outlet. The air inlet and the air outlet of the porous device are interconnected, and the air inlets of the porous device are interconnected, and the air outlets of the porous device are interconnected.

[0068] like Figure 2 The figure shows a schematic diagram of the structure of a second connecting component or a third connecting component. Figure 2 The description is made by taking the example that the second connecting component or the third connecting component includes an air inlet a1 and five air outlets; wherein the air outlets are b1, b2, b3, b4 and b5 respectively.

[0069] For example, the structure of the first connecting member is Figure 2 The second connecting member or the third connecting member shown is the same, Figure 2 The five air outlets b1, b2, b3, b4 and b5 can be used as air inlets of the first connecting member, respectively. Figure 2 The air inlet a1 in the first connecting component can be used as the air outlet of the first connecting component.

[0070] Based on this, the memory effect can be reduced by reducing the volume of the porous device, that is, reducing the length of the air outlet of the first connecting component and the air inlet of the second connecting component.

[0071] For example, the porous device includes an air inlet and 112 air outlets; the internal volume of the porous device is 1.5 ml, the outer diameter is 35 mm, the length is 120 mm, and the inner diameter is 4 mm; the 112 air outlets are all connected to the inner diameter of 4 mm.

[0072] Furthermore, the length of the gas duct between the porous device and the carbon dioxide analyzer can be reduced to reduce the influence of the measured gas in the previous culture bottle on the culture bottle being measured.

[0073] Preferably, the multi-channel control system also includes a first filter, one end of which is connected to the other end of the first air pump, and the other end of the first filter is directly connected to the atmosphere; in this way, solid particles in the atmosphere can be filtered through the first filter, thereby reducing the damage of solid particles in the atmosphere to the automatic microbial respiration rate measurement device provided in an embodiment of the present invention.

[0074] In order to facilitate the extraction of the measured gas in the culture bottle into the carbon dioxide analyzer and improve the accuracy of measuring the respiration rate of the microorganisms, preferably, the multi-channel control system also includes a second air pump, a second filter and an air duct.

[0075] The carbon dioxide analyzer is connected to the air inlet solenoid valve group and the air outlet solenoid valve group through the air guide tube, and the culture bottle is connected to the air inlet solenoid valve and the air outlet solenoid valve through the air guide tube.

[0076] The third connecting component is connected to the air outlet solenoid valve group and the first air pump respectively through the air guide pipe.

[0077] The second air pump is arranged between the air intake solenoid valve group and the carbon dioxide analyzer.

[0078] The second filter is arranged between the second air pump and the carbon dioxide analyzer, and is used to reduce the damage of the solid particles in the culture bottle to the microbial respiration rate automatic determination device provided by the embodiment of the present invention, and to improve the accuracy of determining the microbial respiration rate. In addition, since ventilating the culture bottle will accelerate the evaporation of soil moisture in the culture bottle, in order to inhibit the evaporation of soil sample moisture in the culture bottle, preferably, a water vapor saturated gas generating device is also included; the water vapor saturated gas generating device includes: an artificial climate box, a copper particle bottle and a buffer bottle;

[0079] The copper particle bottle and the buffer bottle are located in the artificial climate chamber; the copper particle bottle is connected to the first air pump and the buffer bottle respectively; the buffer bottle is also connected to the third connecting component; the water vapor saturation gas generating device is used to adjust the water vapor concentration of the atmosphere to a saturated state. Figure 3 , which is a schematic structural diagram of a water vapor saturated gas generating device provided in an embodiment of the present invention.

[0080] The temperature of the climate box can be preset, and the temperature of the climate box is the culture temperature of microbial respiration; the copper particle bottle includes copper particles and a small amount of pure water.

[0081] In actual application, the buffer bottle is connected to the air outlet solenoid valve group of the multi-channel control system through the third connecting component, so that the water vapor saturated atmosphere generated by the water vapor saturation generating device is transported to the culture bottle through the first air pump, and discharged to the outside of the culture bottle through the ventilation port m3 of the air inlet solenoid valve.

[0082] Since copper has a high thermal conductivity and a low specific heat capacity, during the temperature change of the climate chamber, the temperature of the copper particles will change rapidly with the temperature of the climate chamber, making the temperature of the copper particle bottle the same as the temperature of the climate chamber.

[0083] In this way, if the temperature in the climate chamber is lower than the temperature of the atmosphere drawn into the copper particle bottle, then the atmosphere entering the copper particle bottle will condense when it encounters the low-temperature copper particles and generate a water vapor saturated atmosphere at the low temperature of the climate chamber. If the temperature in the artificial climate chamber is higher than the temperature of the atmosphere drawn into the copper particle bottle, then the atmosphere entering the climate chamber will significantly increase the water vapor concentration of the atmosphere in the copper particle bottle after passing through the mixture of the copper particle bottle and water until it is close to saturation, that is, generating a water vapor saturated atmosphere at the high temperature of the climate chamber; then it will enter the multi-channel control system through the buffer bottle, and then ventilate the culture bottle to prevent the moisture in the water vapor saturated atmosphere from bringing the moisture in the copper particle bottle into the multi-channel control system, thereby reducing the error in the respiratory rate of the collected microorganisms caused by water vapor. In this way, while maintaining the normal concentration of carbon dioxide in the culture bottle, the rate of soil evaporation and soil drying is greatly reduced.

[0084] Preferably, the culture bottle is located in an artificial climate chamber; the artificial climate chamber is also used to change the temperature of the culture bottle, and determine the temperature sensitivity of microbial respiration by measuring the microbial respiration rate at different temperatures.

[0085] In practical applications, the temperature sensitivity of microbial respiration can be determined by setting an automatic temperature change program in the artificial climate chamber and measuring the microbial respiration rate at different temperatures.

[0086] Among them, temperature sensitivity refers to the response index of microbial respiration to temperature increase; the temperature sensitivity of soil microbial respiration plays a very critical role in global carbon cycle simulation and prediction of the impact of future global warming on the carbon cycle. In practical applications, the temperature of the climate chamber where the culture bottle is located can be continuously adjusted to continuously measure the microbial respiration rate of each culture bottle at different temperatures, thereby determining the temperature sensitivity of the microorganisms, that is, the relationship between temperature and microbial respiration rate.

[0087] Preferably, a T-shaped three-way component is provided in the culture bottle, which is connected to the multi-channel control system and is used to adjust the flow direction of the measured gas to a horizontal direction.

[0088] The T-shaped three-way component includes three vents, namely, vent 1, vent 2 and vent 3. Vent 2 and vent 3 are located on the same horizontal line, and vent 1 is perpendicular to vent 2 and vent 3. In actual applications, vent 1 is connected to the air inlet of the air inlet solenoid valve and the air outlet of the air outlet solenoid valve through air guide pipes.

[0089] In this way, the measured gas sent back to the culture bottle by the gas outlet solenoid valve enters the culture bottle in a direction parallel to the soil surface, and does not blow directly toward the soil, thereby reducing evaporation on the soil surface of the culture bottle.

[0090] Furthermore, the automatic determination device for the respiration rate of microorganisms provided in the embodiment of the present invention also includes a power supply module, which is electrically connected to the multi-channel control system and the carbon dioxide analyzer. The power supply module is used to supply power to the carbon dioxide analyzer and the multi-channel control system; the power supply system is electrically connected to the carbon dioxide analyzer and the multi-channel control system. It should be noted that the embodiment of the present invention does not limit the power supply method of the power supply system.

[0091] It should be noted that the embodiment of the present invention does not limit the pore size of the first filter and the second filter. For example, the pore size of the first filter and the second filter are respectively between 0.2-1 micron. There is no limitation on the flow rate of the first air pump and the flow rate of the second air pump. For example, the flow rate of the first air pump is between 1L / min, and the flow rate of the second air pump is determined according to the number of channels, ensuring that the average flow rate of each channel is about 0.5L / min.

[0092] In the embodiment of the present invention, when a large number of soil samples are continuously and automatically measured, the large number of soil samples are respectively placed in culture bottles, and the channels of the multi-channel control system are connected to each culture bottle one by one, so as to extract the measured gas in each culture bottle in turn and transport the measured gas to the carbon dioxide analyzer, and then transport the measured gas back to the original culture bottle; the carbon dioxide analyzer obtains carbon dioxide concentration data based on the measured gas, and sends the obtained carbon dioxide concentration data to the data collector, so that the data collector determines the microbial respiration rate based on the carbon dioxide concentration data; in this way, the embodiment of the present invention can realize continuous measurement of a large number of soil samples by expanding the number of channels of the control system, and there is no need to manually replace the next batch of culture bottles after measuring a batch of culture bottles, thereby reducing the interference of obtaining the microbial respiration rate due to manual switching, and improving the efficiency and accuracy of the microbial respiration rate measurement.

[0093] Furthermore, the air inlet solenoid valve provided by the present invention further includes a ventilation port m3, and the air outlet solenoid valve further includes a ventilation port n3, which is used to ventilate the culture product before measuring the microbial respiration rate of the culture bottle, so that the carbon dioxide concentration of the measured gas is the same as the atmospheric carbon dioxide concentration, thereby improving the accuracy of measuring the microbial respiration rate. Further, the microbial respiration rate automatic measurement device provided by the present invention also includes a water vapor saturation generation device to suppress the evaporation of soil moisture caused by the ventilation process of the culture bottle.

[0094] Furthermore, the present invention can determine the temperature sensitivity of microbial respiration by measuring the microbial respiration rate at different temperatures by setting an automatic temperature change program of the artificial climate box.

[0095] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0096] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. An automatic measuring device for microbial respiration rate, characterized in that: include: At least one culture flask; A multi-channel control system, comprising at least one channel, wherein the channel is correspondingly connected to the culture bottle, and the channel is used to obtain the measured gas in the culture bottle; A carbon dioxide analyzer, connected to the multi-channel control system, for measuring carbon dioxide concentration data in the measured gas; A data collector connected to the carbon dioxide analyzer, used to collect the carbon dioxide concentration data and determine the microbial respiration rate based on the carbon dioxide concentration data; The multi-channel control system comprises: An air intake solenoid valve group, comprising at least one air intake solenoid valve; the air intake solenoid valve is connected to the culture bottle correspondingly, and is connected to the carbon dioxide analyzer through a first connecting component, and is used to transport the measured gas from the culture bottle to the carbon dioxide analyzer; An outlet solenoid valve group, comprising at least one outlet solenoid valve; the outlet solenoid valve is connected to the culture bottle correspondingly, and is connected to the carbon dioxide analyzer through a second connecting component, and is used to transport the measured gas from the carbon dioxide analyzer back to the culture bottle; and is connected to one end of the first air pump through a third connecting component, and is used to transport the atmosphere to the culture bottle to perform a ventilation operation; The number of the air inlet solenoid valves, the number of the air outlet solenoid valves and the number of the culture bottles are the same; At least one multi-channel time relay, electrically connected to the air intake solenoid valve and the air outlet solenoid valve, respectively, for controlling the working sequence of the air intake solenoid valve and the air outlet solenoid valve; Wherein, the air inlet solenoid valve and the air outlet solenoid valve connected to the same culture bottle are interconnected through the carbon dioxide analyzer, and the air inlet solenoid valve and the air outlet solenoid valve connected to different culture bottles are not interconnected; The air inlet solenoid valve and the air outlet solenoid valve measure the microbial respiration rate when powered on, and perform ventilation when powered off. At the same time, only one culture bottle is measuring the microbial respiration, and all other culture bottles are performing ventilation operations; The multi-channel time relay controls the operation of the air intake solenoid valve and the air outlet solenoid valve, specifically: The multi-channel time relay supplies power to the No. 1 air inlet solenoid valve and the No. 1 air outlet solenoid valve at the same time, and performs microbial respiration measurement on the No. 1 culture bottle, that is, the measured gas in the No. 1 culture bottle is pumped into the carbon dioxide analyzer through the No. 1 air inlet solenoid valve, and then returns to the No. 1 culture bottle through the No. 1 air outlet solenoid valve; at the same time, all culture bottles except the No. 1 culture bottle perform ventilation operation; after one measurement cycle, the No. 1 air inlet solenoid valve and the No. 1 air outlet solenoid valve are powered off; Among them, the air inlet solenoid valve i and the air outlet solenoid valve i are respectively connected to the i-th culture bottle; i is greater than or equal to 1, and i is a positive integer; After the No. 1 air inlet solenoid valve and the No. 1 air outlet solenoid valve are powered off, the multi-channel time relay supplies power to the No. 2 air inlet solenoid valve and the No. 2 air outlet solenoid valve at the same time, and performs microbial respiration measurement on the No. 2 culture bottle, that is, the measured gas in the No. 2 culture bottle is drawn into the carbon dioxide analyzer through the No. 2 air inlet solenoid valve, and then returns to the No. 2 culture bottle through the No. 2 air outlet solenoid valve; at the same time, all cultures except the No. 2 culture bottle perform ventilation operations, and so on until the microbial respiration measurement of all culture bottles is completed.

2. The automatic measuring device for microbial respiration rate according to claim 1, characterized in that: The first connecting component includes an air outlet and at least one air inlet; The second connecting member includes an air inlet and at least one air outlet; The third connecting component includes an air inlet and at least one air outlet.

3. The automatic measuring device for microbial respiration rate according to claim 1, characterized in that: The multi-channel control system further comprises a first filter, one end of which is connected to the other end of the first air pump, and the other end of the first filter is directly connected to the atmosphere.

4. The automatic measuring device for microbial respiration rate according to claim 1, characterized in that: The multi-channel control system further includes a second air pump, a second filter and an air guide tube; The carbon dioxide analyzer is connected to the air inlet solenoid valve group and the air outlet solenoid valve group through the air guide pipe, and the culture bottle is connected to the air inlet solenoid valve and the air outlet solenoid valve through the air guide pipe; The third connecting component is connected to the air outlet solenoid valve group and the first air pump respectively through the air guide pipe; The second air pump is arranged between the air intake solenoid valve group and the carbon dioxide analyzer; The second filter is disposed between the second air pump and the carbon dioxide analyzer.

5. The automatic measuring device for microbial respiration rate according to claim 1, characterized in that: It also includes a water vapor saturated gas generating device; the water vapor saturated gas generating device includes: an artificial climate box, a copper particle bottle and a buffer bottle; The copper particle bottle and the buffer bottle are located in the artificial climate box; the copper particle bottle is connected to the first air pump and the buffer bottle respectively; the buffer bottle is also connected to the third connecting component; the water vapor saturation gas generating device is used to adjust the water vapor concentration of the atmosphere to a saturated state.

6. The automatic measuring device for microbial respiration rate according to claim 5, characterized in that: The culture bottle is located in the artificial climate box; the artificial climate box is also used to change the temperature of the culture bottle, and the temperature sensitivity of microbial respiration is determined by measuring the microbial respiration rate at different temperatures.

7. The automatic measuring device for microbial respiration rate according to any one of claims 1 to 6, characterized in that: The culture bottle is provided with a T-shaped three-way component, which is connected to the multi-channel control system and is used to adjust the flow direction of the measured gas to a horizontal direction.

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