Detection device and detection method

Abstract

The embodiments of the present invention provide a detection device and a detection method. The detection device includes a secondary sample loading structure. The secondary sample loading structure includes a accommodating device and an opening component. Through the secondary sample loading structure, various functional extensions can be performed for various test strips. The accommodating device can be used to accommodate functional reagents, and the opening component that can be opened in a precisely controlled manner can be used to release the functional reagents. The functional reagents can be released at the precise position of the test strip and at the precise process node. This can expand the detection function of the test strip and ensure the accuracy of the detection results.

Description

Technical Field

[0001] Embodiments of the present invention relate to the field of detection technology, and more specifically, embodiments of the present invention relate to a detection device and a detection method for detecting a sample using a test paper. Background Art

[0002] This section is intended to provide a background or context to embodiments of the invention that are recited in the claims. No description herein is admitted to be prior art by inclusion in this section.

[0003] The test strip detection device is generally used to detect the color information of the test strip detection line, and the concentration level of the target substance can be obtained based on the color information.

[0004] The test paper generally contains a reactant (T line position). When the sample liquid is added to the test paper, the sample liquid can slowly climb to the T line position and the C line position. The reactant and the target substance in the sample liquid react to develop color at the T line position. Normally, the test line T can be displayed. When the sample liquid climbs to the C line position, the quality control line C at the C line position develops color. When the quality control line C is valid, the test paper detection device obtains the concentration level of the target substance by detecting the color information of the test line T.

[0005] However, in some cases, it is difficult to obtain accurate detection data based on the color signal because the color development concentration of the detection line T is too low. For some samples with poor color development reaction, it may not be possible to detect them through the test paper detection device.

[0006] According to the inventors' current understanding, the test results can be enhanced and colored by adding functional reagents such as sensitizers. However, these functional reagents can only be used under laboratory conditions due to the lack of stable carriers, and cannot be used for ordinary consumers or large-scale industrial applications. Summary of the invention

[0007] It can be seen that due to the lack of a stable carrier, various secondary added functional reagents cannot be well applied when the test paper device of the prior art is used for detection.

[0008] Therefore, in the prior art, the detection device has a poor color reaction, which is a very annoying process.

[0009] Therefore, an improved detection device is greatly needed to provide a stable and implementable carrier for various functional reagents, so as to refill the detection structure and quickly obtain accurate detection data.

[0010] In this context, embodiments of the present invention contemplate providing a detection device.

[0011] In a first aspect of an embodiment of the present invention, a detection device is provided, which uses a test paper to detect a sample, wherein the test paper has a primary sample loading area and a detection color development area; the detection device has a secondary sample loading structure, and the secondary sample loading structure includes a accommodating device and an opening component; a accommodating cavity is formed in the accommodating device, and the accommodating cavity accommodates a secondary sample loading agent; the accommodating device has a controllable opening facing the test paper; the controllable opening is aligned with the secondary sample loading area of ​​the test paper; the opening component controls the opening of the controllable opening to release the secondary sample loading agent in the accommodating cavity to the test paper.

[0012] In some embodiments, the secondary sample application area according to any of the above embodiments of the present invention is located between the detection color development area and the primary sample application area.

[0013] In some embodiments, the controllable opening according to any of the above embodiments of the present invention comprises an outflow wall.

[0014] In some embodiments, the outflow wall according to any of the above embodiments of the present invention is a wall body formed with grooves; or, the outflow wall is a thin film structure.

[0015] In some embodiments, the opening component according to any of the above embodiments of the present invention includes a stopper portion, which corresponds to and faces the outflow wall; after the opening component is driven, the stopper portion breaks the outflow wall to release the secondary sample agent in the accommodating chamber to the test paper.

[0016] In some embodiments, the abutment portion according to any of the above embodiments of the present invention has a front end cone structure, and the cone structure tapers from a first end to the other end facing the outflow wall.

[0017] In some embodiments, one or more guide grooves are formed on the outer periphery of the abutting top portion described in any of the above embodiments of the present invention, and the guide grooves extend toward the direction of the test paper.

[0018] In some embodiments, the cross-sectional shape of the guide groove according to any of the above embodiments of the present invention is V-shaped or U-shaped.

[0019] In some embodiments, the detection device according to any of the above embodiments of the present invention further includes a water-permeable sheet, which carries a second secondary sample addition agent; after the abutting top portion breaks the outflow wall, the secondary sample addition agent in the accommodating device is mixed with the second secondary sample addition agent and then released to the test paper.

[0020] In some embodiments, the detection device according to any of the above embodiments of the present invention further comprises a bracket, and the bracket carries the accommodating device.

[0021] In some embodiments, the bracket according to any of the above embodiments of the present invention further has an overhead portion, the overhead portion forms an overhead space for the test paper to pass through, and the overhead space separates the accommodating device and the test paper by a certain distance.

[0022] In some embodiments, the accommodation device according to any of the above embodiments of the present invention can be assembled to the bracket through a connecting mechanism.

[0023] In some embodiments, the detection device according to any of the above embodiments of the present invention further includes a water permeable sheet, the bracket has a release channel, the water permeable sheet is located at the upper part of the release channel, and the release channel is open to face the test paper; after the abutting top portion breaks through the outflow wall, the abutting top portion abuts against the water permeable sheet, so that the water permeable sheet moves downward toward or contacts the test paper through the release channel, and the secondary sample agent is released to the test paper through the water permeable sheet.

[0024] In some embodiments, the bracket described in any of the above embodiments of the present invention has a bearing platform for bearing the water-permeable sheet, and the bearing platform is located on the upper part of the release channel to form a step.

[0025] In some embodiments, the release channel described in any of the above embodiments of the present invention is provided with a guide groove corresponding to the abutting top portion to guide the abutting top portion in a directional manner.

[0026] In some embodiments, the water-permeable sheet according to any of the above embodiments of the present invention is natural fiber filter paper, chemical fiber filter paper or glass fiber filter paper.

[0027] In some embodiments, the detection color development area according to any of the above embodiments of the present invention includes a quality control color development area and a detection result color development area; when the quality control color development area is normally colored and / or the color significance parameter of the detection result color development area is lower than a predetermined threshold, the opening component is driven to move for secondary sampling.

[0028] In some embodiments, the accommodating cavity described in any of the above embodiments of the present invention has an open end, the opening component is located in the accommodating cavity, and the opening component further includes a main body, which is movably installed in the accommodating cavity to form a sealed cavity, and an external driving structure drives the opening component to move through the open end.

[0029] In some embodiments, the accommodating cavity according to any of the above embodiments of the present invention is a cylindrical cavity, the main body of the opening component is a shape adapted to the cylindrical cavity, and the main body of the opening component is provided with at least one layer of sealing ring.

[0030] In some embodiments, the accommodating device according to any of the above embodiments of the present invention has a flexible shell, and the opening component is driven to move by applying pressure to the flexible shell.

[0031] In some embodiments, the detection device described in any of the above embodiments of the present invention also includes a shell, which is provided with a sample loading port, an observation window and a secondary sample loading operation interface; the sample loading port is aligned with the primary sample loading area of ​​the test paper; the observation window is aligned with the detection color development area of ​​the test paper; the secondary sample loading structure is installed on the shell through the secondary sample loading operation interface.

[0032] In some embodiments, the secondary sample loading operation interface according to any of the above embodiments of the present invention is an open opening, and the accommodating device is assembled and connected with the bracket in the open opening through the open opening.

[0033] In some embodiments, the housing according to any of the above embodiments of the present invention comprises a first housing and a second housing, wherein the first housing and the second housing are connected and combined to form a cavity therebetween for accommodating the test paper.

[0034] In some embodiments, the secondary loading agent according to any of the above embodiments of the present invention is a sensitizer.

[0035] In a second aspect of the embodiments of the present invention, a detection method is provided, comprising the following steps:

[0036] Prepare a test paper, the test paper having a primary sample addition area and a detection color development area;

[0037] Preparing a container carrying a secondary sample addition agent, wherein the container has a controllable opening facing the test paper;

[0038] Adding a sample once to the primary sample adding area;

[0039] Aligning the controllable opening with the secondary sample addition area of ​​the test paper;

[0040] The controllable opening of the container is controlled to open so as to release the secondary sample addition agent to the test paper, so as to perform secondary sample addition on the test paper after the primary sample addition.

[0041] In some embodiments, according to any of the above embodiments of the present invention, a water-permeable sheet is provided to carry another secondary sample addition agent; after a single sample addition test is completed, the water-permeable sheet is placed on the test paper, and the secondary sample addition agent in the container is added to the water-permeable sheet, so that the secondary sample addition agent in the container is mixed with another secondary sample addition agent in the water-permeable sheet and then released to the test paper.

[0042] In some embodiments, according to any of the above embodiments of the present invention, the secondary sample addition agent is a sensitizer, which performs sensitization and color development on the detection result of the primary sample addition.

[0043] In some embodiments, according to any of the above embodiments of the present invention, the controllable opening includes an outflow wall, wherein controlling the opening of the controllable opening of the container includes using the top of the opening component to break the outflow wall to release the secondary sample agent to the test paper.

[0044] In some embodiments, according to any of the above embodiments of the present invention, it further includes providing one or more guide grooves on the outer periphery of the abutment top to guide the secondary sample addition agent to the test paper.

[0045] In some embodiments, according to any of the above embodiments of the present invention, the method further comprises using a sealing member to movably seal the opening component to a cylindrical container having an open end.

[0046] In some embodiments, according to any of the above embodiments of the present invention, it further includes applying pressure to a sealed container having a flexible shell to drive the opening component to move so that the abutting top portion breaks through the outflow wall.

[0047] In some embodiments, according to any of the above embodiments of the present invention, further comprising:

[0048] Placing the water-permeable sheet on a step so that the water-permeable sheet is separated from the test paper by a certain distance;

[0049] After the abutting top portion breaks through the outflow wall, the abutting top portion abuts against the water permeable sheet, so that the water permeable sheet contacts the test paper downward, and the secondary sample addition agent is released to the test paper through the water permeable sheet.

[0050] In some embodiments, according to any of the above embodiments of the present invention, wherein the detection color development area includes a quality control color development area and a detection result color development area, the method further includes driving the opening component to move for secondary sampling when the quality control color development area is normally colored and / or the color significance parameter of the detection result color development area is lower than a predetermined threshold.

[0051] According to the detection device and detection method of the embodiment of the present invention, those skilled in the art should understand that, compared with the prior art, a secondary sample addition structure is provided for the test paper, and various functional extensions can be performed for various test papers, and a containing device can be used to contain functional reagents, and a controllably opened opening component can be used to release the functional reagents; the functional reagents can be released at the exact position of the test paper and at the precise process node, so as to expand the detection function of the test paper and ensure the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] The above and other objects, features and advantages of the exemplary embodiments of the present invention will become readily understood by reading the detailed description below with reference to the accompanying drawings. In the accompanying drawings, several embodiments of the present invention are shown in an exemplary and non-limiting manner, in which:

[0053] Figure 1 The structure diagram of the detection device according to the first embodiment of the present invention is schematically shown;

[0054] Figure 2 Schematically shows the Figure 1 Schematic diagram of the cross-sectional structure of line AA in FIG.

[0055] Figure 3 The cross-sectional structure diagram of the secondary sample loading structure according to the first embodiment of the present invention is schematically shown;

[0056] Figure 4 Schematically shows a structural diagram of an opening component according to a first embodiment of the present invention;

[0057] Figure 5 The schematic diagram shows the use process of the detection device according to the first embodiment of the present invention. Figure 1 ;

[0058] Figure 6 The schematic diagram shows the use process of the detection device according to the first embodiment of the present invention. Figure 2 ;

[0059] Figure 7 The structure diagram of the detection device according to the second embodiment of the present invention is schematically shown;

[0060] Figure 8 A schematic cross-sectional structural diagram of a detection device according to a third embodiment of the present invention is shown;

[0061] Fig. 9 The cross-sectional structure diagram of the secondary sample adding structure according to the third embodiment of the present invention is schematically shown.

[0062] In the drawings, the same or corresponding reference numerals represent the same or corresponding parts.

[0063] 1. Test paper; 11. Primary sample loading area; 12. Detection color development area; 121. Quality control color development area; 122. Detection result color development area; 13. Sample pad; 14. Water-absorbing filter paper; 15. Colloidal gold pad; 100. Secondary sample loading structure; 2. Bracket; 21. Carrying platform; 22. Release channel; 23. Guide groove; 24. Overhead; 25. Connection interface; 3. Accommodation device; 30. Controllable opening; 31. Accommodation cavity; 32. Open end; 33. Flow Outlet wall; 34, outer wall; 35, sealing plate; 36, connecting mechanism; 4, opening component; 41, main body; 411, fixing groove; 412, sealing ring; 42, top support; 421, vertebral ridge; 422, guide groove; 5, secondary sample adding agent; 6, water-permeable sheet; 70, bottom frame; 7, shell; 71, sample adding port; 72, observation window; 73, secondary sample adding operation interface; 74, first shell; 75, second shell; 76, installation area; 8, pressure rod.

[0064] 3', accommodating device; 31', accommodating chamber; 32', flexible shell; 33', outflow wall; 34', internal part. DETAILED DESCRIPTION

[0065] The principles and spirit of the present invention will be described below with reference to several exemplary embodiments. It should be understood that these embodiments are provided only to enable those skilled in the art to better understand and implement the present invention, and are not intended to limit the scope of the present invention in any way. On the contrary, these embodiments are provided to make the present disclosure more thorough and complete, and to fully convey the scope of the present disclosure to those skilled in the art.

[0066] According to an embodiment of the present invention, a detection device and a detection method are provided.

[0067] In this article, it is to be understood that the term test paper refers to a type of paper impregnated with chemicals that can detect the presence of certain substances in liquids or gases through their color changes. For example, a dry paper strip impregnated with an indicator or reagent. Another example is a colloidal gold test paper, which generally mainly includes a sample pad, a colloidal gold pad, a carrier and a water-absorbing filter paper from one end to the other. These parts can also be fixed on a base material. The above-mentioned carrier can be understood as a detection color development area. In addition, any number of elements in the accompanying drawings is used for example rather than limitation, and any naming is only used for distinction and does not have any limiting meaning.

[0068] The principle and spirit of the present invention are explained in detail below with reference to several representative embodiments of the present invention. SUMMARY OF THE INVENTION

[0070] The inventors have found that in some cases, it is difficult to obtain accurate detection data based on the color signal because the test line concentration of the test paper is too low. For some samples with poor color development reaction, it may not be possible to detect them using the test paper detection device.

[0071] Therefore, it is necessary to set up a secondary sample addition structure for the test paper, which can be used to expand the functions of various test papers. The containing device can be used to contain the functional reagents, and the opening parts that can be opened in a controlled manner can be used to release the functional reagents; the functional reagents can be released at the exact position of the test paper and at the precise process node. This can expand the detection function of the test paper and ensure the accuracy of the test results.

[0072] After introducing the basic principles of the present invention, various non-limiting embodiments of the present invention are described in detail below.

[0073] Exemplary Methods

[0074] The embodiments of the present invention can be applied to any applicable scenario.

[0075] The embodiment of the present invention provides a detection method, which can be considered to mainly include the following steps:

[0076] Prepare a test paper, the test paper has a primary sample addition area and a detection color development area;

[0077] Preparing a container carrying a secondary sample addition agent, the container having a controllable opening facing the test paper;

[0078] Apply the sample once to the test strip;

[0079] After the first sampling test is completed, the controllable opening of the container is controlled to open to release the secondary sampling agent to the test paper, so as to perform a second sampling on the test paper after the first sampling test. The so-called secondary sampling here refers to the action of adding samples or other functional reagents to the test paper carrier again after the first sampling using the object sample. The secondary sampling agent can be understood as adding the original object sample or various functional reagents to the test paper, such as masking agents, color developers, sensitizers or diluents, etc., which may be added twice or multiple times.

[0080] In another specific operation example, a water-permeable sheet can also be provided to carry another secondary sample addition agent; after the first sample addition test is completed, the water-permeable sheet is placed on the test paper, and the secondary sample addition agent in the container is added to the water-permeable sheet to be mixed with another secondary sample addition agent in the water-permeable sheet and then released to the test paper. The water-permeable sheet is a material that allows liquid to pass through, and can be selected as natural fiber filter paper, chemical fiber filter paper or glass fiber filter paper, but is not limited to this. In a specific practical example, the water-permeable sheet is selected as glass fiber filter paper. In use, the water-permeable sheet can evenly guide the secondary sample addition agent to the test paper.

[0081] The secondary sample addition agent may be a sensitizer, so as to facilitate the sensitization and color development of the detection result of the primary sample addition. According to a specific application example of the present invention, the sensitizer may also include two or more components stored separately. The specific carrying method has been introduced in the above structural example and will not be repeated here. This high-efficiency sensitizer mixed during use can further improve the sensitization efficiency. If there is a need to use three or more components of sensitizers, it can be considered to place more permeable sheets, and each permeable sheet can carry different components. Referring to the above description, it should be understood by those skilled in the art that, relative to the prior art, with the detection method of the above embodiment, a secondary sample addition operation is set for the test paper, and various functional extensions can be performed for various test papers. The container can be used to accommodate the functional reagent, and the container can be opened in a precisely controlled manner to release the functional reagent; the functional reagent can be released at the exact position of the test paper and at the precise process node. To expand the detection function of the test paper and ensure the accuracy of the test results.

[0082] Exemplary Devices

[0083] After introducing the method of the exemplary embodiment of the present invention, next, refer to Figures 1 to 9 The detection device according to the exemplary embodiment of the present invention is described as follows:

[0084] First embodiment

[0085] Figure 1 The structure diagram of the detection device according to the first embodiment of the present invention is schematically shown; Figure 2 Schematically shows the Figure 1 A schematic cross-sectional view of a portion of the structure along line AA in FIG. Figure 3 The cross-sectional structure diagram of the secondary sample loading structure in the first embodiment of the present invention is schematically shown.

[0086] like Figures 1 to 3 As shown, the first embodiment of the present invention provides a detection device, which can be used to detect corresponding object samples with various test papers. It should be understood that the test paper 1 generally has a primary sample addition area 11 and a detection color development area 12. The primary sample addition area 11 can be understood as an area for adding reagents such as samples, which is generally located at one end of the test paper strip, such as a sample pad 13 connected to the detection color development area 12; it can be understood that the sample pad 13 can also be integrated with the detection color development area 12. The detection color development area 12 can be understood as a carrier of the detection reagent, which is used to carry the detection reagent. The material of the detection color development area 12 is, for example, filter paper, nitrocellulose membrane, and microporous filter membrane. The detection color development area 12 generally includes a quality control color development area 121 and a test result color development area 122. A water-absorbing filter paper 14 can be connected to the other end of the detection color development area 12. The above-mentioned test paper 1 can be assembled and fixed using a base frame 70, and the base frame 70 can be arranged with corresponding connection and positioning components.

[0087] In this embodiment, the detection device also has a secondary sample loading structure 100 (see Figure 3 ), the secondary loading structure 100 may mainly include a containing device 3 and an opening component 4. So as to facilitate the secondary loading of the test paper 1 using the secondary loading structure 100, so as to realize the secondary loading as required. The so-called secondary loading here refers to the action of adding samples or other functional reagents to the test paper carrier again after the object sample is loaded for the first time. The reagents for secondary loading can be understood as the original object sample or various functional reagents added to the test paper, such as masking agents, color developers, sensitizers or diluents, etc., which may be loaded twice or multiple times.

[0088] In one embodiment, the first addition is used to add the target sample to be tested, and the second addition is used to add a sensitizer to increase the color significance of the detection line.

[0089] In one embodiment, the secondary sample loading structure 100 may also be provided with a bracket 2 for assembly with the accommodating device 3. The secondary sample loading structure 100 may be fixed to the base frame 70, and may be detachably assembled to the base frame 70 with a connecting piece at the bottom of the bracket 2. The connecting piece may be assembled to the base frame 70 by screwing, clamping, riveting, welding, etc., and the clamping method is used as an example in the figure.

[0090] In one embodiment, the bracket 2 is selectively arranged between the detection color development area 12 and the primary sample loading area 11, that is, located on the area between the detection color development area 12 and the primary sample loading area 11 of the test paper 1 along the length direction of the test paper 1. So that secondary loading can be performed at the position between the detection color development area 12 and the primary sample loading area 11. It should be understood that the secondary loading is performed between the detection color development area 12 and the primary sample loading area 11, and the reagents for the secondary loading have the same chromatography direction as the sample. First, they can enter the detection color development area 12 normally; second, the chromatography process can be fully mixed with the primary loading sample; third, the primary loading area 11 and the secondary loading structure 100 will not interfere with each other; fourth, the primary loading and the secondary loading can be effectively distinguished to avoid misoperation.

[0091] Those skilled in the art should understand that the location of the secondary loading structure for loading the test paper for the secondary loading can be other than between the detection color development area 12 and the primary loading area 11 as described above, such as the detection color development area or to the target area via other indirect components. Taking colloidal gold test paper as an example, colloidal gold pads are generally provided between the primary loading area and the detection color development area of ​​the colloidal gold test paper, and the secondary loading structure can choose to align the colloidal gold pads for secondary loading.

[0092] The bracket 2 is used to carry and fix the accommodating device 3, and can also make a certain distance between the accommodating device 3 and the test paper 1. It should be understood by those skilled in the art that the bracket 2 is only used to fix the accommodating device 3, and its structural form is not limited. By using the setting position of the bracket 2, the precise position of the secondary addition of the test paper can be determined. And by using the spacing effect of the bracket 2, it can be ensured that the detection or chromatography process of the primary addition will not be affected when the secondary addition is not performed, thereby avoiding affecting the primary addition detection. In one embodiment, the secondary addition structure 100 may not include an independent bracket structure, and the secondary addition structure can, for example, carry and install the accommodating device through an integrally formed shell for accommodating the test paper.

[0093] In this embodiment, a receiving chamber 31 may be formed in the receiving device 3 so as to receive a secondary sample addition agent 5 (see Figure 3 ). The containing device 3 may be provided with a controllable opening 30 facing the test paper 1. The containing device 3 is mainly used to contain the secondary sample agent 5, so the containing device 3 may be various shapes or structures that meet the function of the container, such as cylindrical, square cylindrical, spherical, ellipsoidal or capsule-shaped container shapes. It should be understood that the controllable opening 30 may be various structures that can be opened in a controlled manner, such as a movable cover structure, a movable sealing structure, a breakable diaphragm structure or a breakable wall structure. Those skilled in the art should understand that various structures that can controllably open a controllable opening can be applied as the opening component 4, and there is no specific limitation here. For example: a direct-acting or swinging component with spikes driven by a reset spring; of course, the reset spring can also be replaced by other accumulators (such as piston or gas accumulators).

[0094] In the specific example shown in the figure, the containing device 3 can be a cylindrical container, wherein the controllable opening 30 at the bottom can include an outflow wall 33, the outside of the containing device 3 is an annular outer wall 34, and the upper open end 32 can be optionally equipped with or connected to a sealing plate 35. And it can be understood that the outflow wall 33 can be controlled to open in the direction of the test paper to facilitate secondary sampling. The outflow wall 33 can be a wall body formed with a groove; or, the outflow wall 33 can be a thin film structure so that the secondary sample agent 5 can be released downward after opening. The secondary sample agent 5 can be understood as the sample of the original object or various functional reagents added to the test paper, such as masking agents, color developers, sensitizers or diluents, etc., which may be filled twice or multiple times.

[0095] The opening member 4 may include a main body 41 and a stopper 42, and the stopper 42 faces the outflow wall 33. After the opening member 4 is driven, the stopper 42 breaks the outflow wall 33 to release the secondary sample addition agent 5 in the accommodating chamber 31 to the test paper 1. The opening member 4 may be located outside the accommodating chamber 31 or inside the accommodating chamber 31.

[0096] In a specific application example, after the first addition of sample, if the quality control color development area 121 is in normal color development, the opening component 4 is driven to move to perform a second addition of sample. In this way, it is ensured that the sample to be tested will not be stained by the water-permeable sheet 6 during the first addition of sample, and the second addition of sample can be prevented from affecting the quality control color development of the test paper, ensuring that the next step of operation is performed after checking whether the test paper is normal.

[0097] With reference to the above description, those skilled in the art should understand that, compared with the prior art, the detection device of the above embodiment is provided with a secondary sample loading structure for the test paper, and various functional extensions can be performed for various test papers, and the secondary sample loading structure can be assembled and connected by a bracket, and the accommodating device can be used to accommodate the functional reagent, and the opening component that can be opened in a precisely controlled manner can be used to release the functional reagent; the functional reagent can be released at the precise position of the test paper and at the precise process node, so as to expand the detection function of the test paper and ensure the accuracy of the detection results.

[0098] The specific structural examples of the components in the secondary sample adding structure 100 are introduced as follows in conjunction with the accompanying drawings. Figures 1 to 3 As shown, the bracket 2 can be selected as a plastic part, such as polypropylene (PP), and can be selected to be injection molded, but it is not limited to this. The bracket 2 can be recessed downward on the upper surface to form a bearing platform 21 and a release channel 22. The size of the bearing platform 21 can be larger than the release channel 22 (radial size) so as to form a step between the two, and the step can movably support the placement of the water-permeable sheet 6. The step can separate the water-permeable sheet from the test paper below by a certain distance, ensuring that the primary sample addition chromatography and color development process are not affected by the secondary sample addition structure. The bearing platform 21 is connected to the release channel 22 to form a channel that runs through the bracket 2 from top to bottom, and this channel can be open to face the test paper 1. After the bracket 2 and the accommodating device 3 are assembled, the bearing platform 21 and the release channel 22 are simultaneously aligned with the outflow wall 33 of the accommodating device 3 and the top 42 of the opening component 4.

[0099] According to the above specific structural example, a guide groove 23 may be further provided on one or both sides of the release channel 22. The guide groove 23 is formed by an inward recess of the outer wall of the release channel 22. The size of the guide groove 23 corresponds to the outer size of the top portion 42, so that after the top portion 42 enters the release channel 22, the guide groove 23 can be used to limit the movement trajectory of the top portion 42 to avoid deviation of the top portion 42 during forward movement.

[0100] According to the above specific structural example, after the abutting top portion 42 is driven to break through the outflow wall 33, the abutting top portion 42 can abut against the water-permeable sheet 6, so that the water-permeable sheet 6 contacts the test paper 1 downward through the release channel 22, so that the secondary sample agent 5 in the accommodating device 3 can be released to the test paper 1 through the water-permeable sheet 6.

[0101] Reference Figure 2 and Figure 3 As shown, in the specific structural example of the bracket 2, the bracket 2 may also have an overhead portion 24, and the overhead portion 24 forms an overhead space for the test paper 1 to pass through, and the overhead space can separate the accommodating device 3 from the test paper 1 by a certain distance. It can ensure that the detection or chromatography process of the primary sample addition will not be affected when the secondary sample addition is not performed, thereby avoiding affecting the primary sample addition detection.

[0102] like Figures 1 to 3 As shown, the bracket 2 may also have a connection interface 25 facing the accommodating device 3 so as to be assembled and connected with the connection mechanism 36 at the bottom of the accommodating device 3. It should be understood by those skilled in the art that the connection method between the two can be selected as screw connection, clamping connection, welding, etc., and the clamping method is used as an example in the figure. As shown in the figure, the connection interface 25 is two clamping holes opened downward from the top surface of the bracket 2, and correspondingly, the connection mechanism 36 at the bottom of the accommodating device 3 is two clamping columns, and the clamping columns of the connection mechanism 36 can also be provided with positioning structures such as bumps.

[0103] According to another specific structural example, the accommodating device 3 can be cylindrical as a whole, the opening component 4 is located in the accommodating cavity 31, the main body 41 of the opening component 4 is a shape adapted to the cylindrical cavity, and at least one fixed groove 411 is provided on the outer periphery of the main body 41, thereby fixing at least one layer of sealing ring 412, whereby the opening component 4 can be movably sealed in the accommodating cavity 31. The main body 41 of the opening component 4 is movably sealed at the open end 32, and the external driving structure drives the opening component 4 to perform piston movement through the open end 32. With this structure, the secondary sample addition agent 5 in the accommodating device 3 can be prevented from leaking outward. By utilizing the siphon principle, the injection amount of the secondary sample addition can also be controlled according to the downward pressure stroke. At the same time, by utilizing the movably sealed structure, the secondary sample addition agent 5 can also be pushed to flow in the direction of the test paper to avoid affecting the filling of the secondary sample addition agent 5 due to the operation angle problem.

[0104] According to Figure 4 In the specific structural example shown, the top portion 42 is a vertebral structure at least at the front end, and the vertebral structure tapers from one end close to the main body 41 of the opening component 4 to the other end. The vertebral structure can be located at the front end of the top portion 42, or it can extend from the main body 41 to the top of the top portion 42. The vertebral structure forms a spike structure to facilitate puncturing the outflow wall 33. The outer periphery of the top portion 42 can have a plurality of vertebral ridges 421 extending downward, and the outer periphery of the top portion 42 can also be formed with one or more guide grooves 422, and the guide grooves 422 are all located between two adjacent vertebral ridges 421. The guide groove 422 can extend from the main body 41 of the opening component 4 to the direction of the test paper 1. The cross-sectional shape of the guide groove 422 can be V-shaped, or the cross-sectional shape of the guide groove 422 can be U-shaped. After puncturing the outflow wall 33, the secondary sample addition agent 5 can be guided out by the guide groove 422 to avoid blockage.

[0105] According to another specific application example, the water permeable sheet 6 can carry a second secondary sample addition agent 5, and the second secondary sample addition agent 5 can be selected to be in a solid state, such as a freeze-dried powder. After the abutting top portion 42 abuts against the water permeable sheet 6 to contact the test paper 1, the secondary sample addition agent 5 in the accommodating device 3 is mixed with the second secondary sample addition agent 5 in the water permeable sheet and then released to the test paper 1. This can meet the needs of special functional reagents, and the mixing is only performed when used, which can prevent the two from deteriorating after long-term mixing.

[0106] The water-permeable sheet 6 can be selected as natural fiber filter paper, chemical fiber filter paper or glass fiber filter paper, but is not limited thereto. In a specific practical example, the water-permeable sheet 6 is selected as glass fiber filter paper. In use, the water-permeable sheet 6 can evenly guide the secondary sample addition agent 5 onto the test paper.

[0107] According to a specific application example of the present invention, the secondary sample adding agent 5 can be selected as a sensitizer, so that after a sample adding test, the sensitizer can be added to the test paper 1 using the secondary sample adding structure 100. When the sensitizer enters the detection area of ​​the test paper, the color concentration of the detection line can be deepened. In this way, the test results can be read accurately. In addition, the detection time can be greatly shortened. After actual testing, the solution of this application example can shorten the average detection time of 12 minutes of the traditional detection method to an average of about 6 minutes.

[0108] According to a specific application example of the present invention, the sensitizer may also include two or more components stored separately. The specific carrying method has been introduced in the above structural example and will not be repeated here. This high-efficiency sensitizer mixed during use can further improve the sensitization efficiency. If there is a need to use a sensitizer with three or more components, it can be considered to place more permeable sheets 6, and each permeable sheet 6 can carry different components.

[0109] Figure 5 The schematic diagram shows the use process of the detection device according to the first embodiment of the present invention. Figure 1 ; Figure 6 The schematic diagram shows the use process of the detection device according to the first embodiment of the present invention. Figure 2 As shown in the figure, the motor-driven sample adding head can be used to drive the opening component 4. Specifically, the sealing plate 35 can be removed first, and a pressure rod 8 can be placed on the accommodating device 3. Then, the sample adding head can be used to press the pressure rod 8 downward to push against the top 42 to break the outflow wall 33, so as to release the secondary sample adding agent 5 in the accommodating chamber 31 to the test paper 1.

[0110] According to an embodiment, when the detection device uses colloidal gold test paper for detection, the secondary sample loading position corresponding to the secondary sample loading structure 100 can be selected to correspond to the colloidal gold pad position of the colloidal gold test paper.

[0111] Second embodiment

[0112] according to Figure 7 As shown, an embodiment of the present invention provides a detection device. Compared with the above-mentioned first embodiment, the detection device also includes a shell 7, and an operating surface of the shell 7 can be provided with a sample loading port 71, an observation window 72 and a secondary sample loading operation interface 73. The sample loading port 71 can be aligned with the primary sample loading area 11 of the test paper 1. The observation window 72 can be aligned with the detection color development area 12 of the test paper 1. The secondary sample loading operation interface 73 is located between the sample loading port 71 and the observation window 72, and the secondary sample loading operation interface 73 can be an open port, and the accommodating device 3 is assembled and connected with the bracket 2 in the open port through the open port. The secondary sample loading structure 100 is installed in the shell 7 through the secondary sample loading operation interface 73. Among them, the main body of the bracket 2 can be installed in the shell 7, and the top of the bracket 2 can protrude from the open port of the secondary sample loading operation interface 73, so that the accommodating device 3 can be assembled and connected to the bracket 2 through the connecting structure. In this way, the detection device can be divided into two parts during packaging and transportation, one part is the component fixedly installed in the shell 7, and the other part is the accommodating device 3. The user assembles the above two parts before use, which can significantly reduce the packaging size and save transportation costs.

[0113] The housing 7 may be made of plastic or other materials, and injection molding may be selected. According to actual production conditions, the housing 7 may include a first housing 74 and a second housing 75, and the first housing 74 and the second housing 75 are docked and combined, and the combination method may be screwed, clamped, riveted, or welded. An installation area 76 may be formed between the first housing 74 and the second housing 75. The test paper 1 and the bracket 2 may be installed in the installation area 76, and the support platform 21 of the bracket 2 is aligned with the secondary sample addition operation interface 73.

[0114] In one embodiment, the support 2 can be regarded as a part of the secondary sample loading structure. In another embodiment, the support 2 can also be regarded as a part of the housing 7, and can be integrally formed with the housing or detachably assembled.

[0115] The test strip detection device may also include an image sensor for capturing the color information of the detection line on the test strip to obtain the detection value. The housing 7 may include an identification pattern such as a QR code, and the image sensor in the detection device may capture and identify the identification to determine the type of the detection device, thereby determining whether to add a sample, the time for adding a sample, the interval after adding a sample to take a picture of the detection line, and other preset information.

[0116] Third embodiment

[0117] according to Figure 8As shown, the embodiment of the present invention provides a detection device. Compared with the first embodiment and the second embodiment, the main difference is that the accommodating device 3' mainly includes a flexible shell 32', an inner part 34' and an outflow wall 33'. The flexible shell 32' and the inner part 34' together form an accommodating cavity 31'. The inner part 34' is a cylindrical structure with a cylindrical space formed inside. The outer periphery of the inner part 34' can form a convex-concave structure. The inner part 34' can be a hard plastic structure, such as polypropylene (PP).

[0118] The flexible shell 32' can be made of a flexible material, such as thermoplastic polyurethane elastomer rubber (TPU); the overall shape of the flexible shell 32' is in the shape of a cap, and the opening of the flexible shell 32' is combined with the outer periphery of the inner part 34', and the combination method can be adhesive or hot-melt connection, and the bonding strength is increased by the convex and concave structure. A cylindrical space is also formed inside the flexible shell 32', which is connected with the cylindrical space inside the inner part 34' to form a receiving cavity 31'. A concave portion can also be set on the top of the flexible shell 32', and the corresponding inner portion can also be a convex portion, which can facilitate external driving and pressing.

[0119] The outflow wall 33' may be combined at the opening of the flexible shell 32' and the inner part 34'. In this embodiment, the outflow wall 33' is a sealing film, and the material may be a composite material or a metal material.

[0120] The opening member 4 can also be selectively disposed in the accommodating cavity 31 ′. The structure of the opening member 4 can refer to the above-mentioned embodiment.

[0121] The housing 7 may be supported by a bracket 2 to hold the accommodating device 3', and the bracket 2 and the accommodating device 3' may be provided with a connection structure, which may be connected by screwing, clamping, riveting, welding, etc. The first housing 74, the second housing 75 and the bracket 2 may also be used to fix and clamp the accommodating device 3'.

[0122] The accommodating device 3' in this embodiment has a better sealing effect, and the secondary sample addition agent 5 in the accommodating chamber 31' cannot overflow. If the accommodating device 3' is fixed and clamped by the first shell 74, the second shell 75 and the bracket 2, and the sample addition port 71 and the observation window 72 are selectively sealed with transparent materials, not only the accommodating device 3' can be waterproof, but also the entire detection device can be waterproof.

[0123] like Fig. 9 As shown in FIG. 1 , according to one embodiment, when the detection device uses colloidal gold test paper for detection, the secondary sample loading position corresponding to the secondary sample loading structure 100 can be selected to correspond to the position of the colloidal gold pad 15 of the colloidal gold test paper.

[0124] It should be noted that, although several types of devices or sub-devices of the detection device are mentioned in the above detailed description, such division is not mandatory. In fact, according to an embodiment of the present invention, the features and functions of two or more devices described above can be embodied in one device. Conversely, the features and functions of one device described above can be further divided into multiple devices to be embodied.

[0125] In addition, although the operations of the method of the present invention are described in a specific order in the drawings, this does not require or imply that the operations must be performed in this specific order, or that all the operations shown must be performed to achieve the desired results. Additionally or alternatively, some steps may be omitted, multiple steps may be combined into one step, and / or one step may be decomposed into multiple steps.

[0126] Although the spirit and principle of the present invention have been described with reference to several specific embodiments, it should be understood that the present invention is not limited to the disclosed specific embodiments, and the division of various aspects does not mean that the features in these aspects cannot be combined to benefit, and such division is only for the convenience of expression. The present invention is intended to cover various modifications and equivalent arrangements included in the spirit and scope of the attached claims.

Claims

1. A detection device, using a test paper to detect a sample, the test paper having a primary sample loading area and a detection color development area; the detection device having a secondary sample loading structure, the secondary sample loading structure including a accommodating device and an opening component; The accommodating device has an accommodating cavity formed therein, and the accommodating cavity accommodates a secondary sample addition agent; the accommodating device has a controllable opening facing the test paper; the controllable opening includes an outflow wall; the outflow wall is a wall body formed with a groove; Alternatively, the outflow wall is a thin film structure; The controllable opening is aligned with the secondary sample adding area of ​​the test paper; The opening component controls the opening of the controllable opening, and the opening component includes an abutting top portion, and the abutting top portion faces the outflow wall; After the opening component is driven, the abutting top portion breaks the outflow wall to release the secondary sample addition agent in the accommodating chamber to the test paper; The detection device also includes a water-permeable sheet and a bracket, the water-permeable sheet carries a second secondary sample addition agent; the bracket carries the accommodating device so that the accommodating device and the test paper are spaced a certain distance apart, the bracket has a release channel, the water-permeable sheet is located at the upper part of the release channel, and the release channel is open to the test paper; after the abutting top portion breaks the outflow wall, the abutting top portion abuts against the water-permeable sheet, so that the water-permeable sheet moves downward toward or contacts the test paper through the release channel, the secondary sample addition agent is uniformly released to the test paper through the water-permeable sheet, and the secondary sample addition agent in the accommodating device is mixed with the second secondary sample addition agent and then released to the test paper.

2. The detection device according to claim 1, wherein: The secondary sample adding area is located between the detection color development area and the primary sample adding area.

3. The detection device according to claim 1, wherein: The abutting top portion has a front end cone structure, and the cone structure tapers from a first end to the other end facing the outflow wall.

4. The detection device according to claim 3, wherein: One or more guide grooves are formed on the outer periphery of the abutting top portion, and the guide grooves extend toward the direction of the test paper.

5. The detection device according to claim 4, wherein: The cross-section of the guide groove is V-shaped or U-shaped.

6. The detection device according to claim 1, wherein: The bracket also has an overhead portion, which forms an overhead space for the test paper to pass through, and the overhead space separates the accommodating device and the test paper by a certain distance.

7. The detection device according to claim 1, wherein: The accommodating device is assembleably connected to the bracket via a connecting mechanism.

8. The detection device according to claim 1, wherein: The support has a bearing platform for bearing the water-permeable sheet, and the bearing platform is located on the upper part of the release channel to form a step.

9. The detection device according to claim 1, wherein: The release channel is provided with a guide groove corresponding to the abutting top portion to guide the abutting top portion in a directional manner.

10. The detection device according to claim 1, wherein: The water-permeable sheet is natural fiber filter paper, chemical fiber filter paper or glass fiber filter paper.

11. The detection device according to claim 1, wherein: The detection color development area includes a quality control color development area and a detection result color development area; when the quality control color development area is normally colored and / or the color significance parameter of the detection result color development area is lower than a predetermined threshold, the opening component is driven to move to perform secondary sample addition.

12. The detection device according to any one of claims 1 to 11, wherein: The accommodating cavity has an open end, the opening component is located in the accommodating cavity, and the opening component further includes a main body, which is movably installed in the accommodating cavity to form a sealed cavity, and an external driving structure drives the opening component to move through the open end.

13. The detection device according to claim 12, wherein: The accommodating cavity is a cylindrical cavity, the main body of the opening component is a shape adapted to the cylindrical cavity, and the main body of the opening component is provided with at least one layer of sealing ring.

14. The detection device according to any one of claims 1 to 11, wherein: The accommodating device has a flexible shell, and the opening component is driven to move by applying pressure to the flexible shell.

15. The detection device according to any one of claims 1 to 11, further comprising a shell, the shell being provided with a sample loading port, an observation window and a secondary sample loading operation interface; the sample loading port is aligned with the primary sample loading area of ​​the test paper; the observation window is aligned with the detection color development area of ​​the test paper; the secondary sample loading structure is installed on the shell through the secondary sample loading operation interface.

16. The detection device according to claim 15, wherein: The secondary sample addition operation interface is an open opening, and the accommodating device is assembled and connected with the bracket in the open opening through the open opening.

17. The detection device according to claim 15, wherein: The housing comprises a first housing and a second housing, and the first housing and the second housing are connected and assembled to form a cavity therebetween for accommodating the test paper.

18. The detection device according to any one of claims 1 to 11, wherein: The secondary sample addition agent is a sensitizer.

19. A detection method comprising the following steps: Prepare a test paper, the test paper having a primary sample addition area and a detection color development area; Prepare a container carrying a secondary sample addition agent, wherein the container has a controllable opening facing the test paper, and the controllable opening includes an outflow wall; Adding a sample once to the primary sample adding area; Aligning the controllable opening with the secondary sample addition area of ​​the test paper; Controlling the opening of the controllable opening of the container to release the secondary sample addition agent to the test paper, so as to perform secondary sample addition on the test paper after the primary sample addition; Wherein, the controlling and opening the controllable opening of the container comprises: using the abutting top portion of the opening component to break the outflow wall, the abutting top portion of the opening component facing the outflow wall; The method also includes: providing a water-permeable sheet carrying another secondary sample adding agent, placing the water-permeable sheet on a step so that the water-permeable sheet is separated from the test paper by a certain distance; after the abutting top portion breaks the outflow wall, the abutting top portion abuts against the water-permeable sheet so that the water-permeable sheet contacts the test paper downward, and the secondary sample adding agent is uniformly released to the test paper through the water-permeable sheet, after a sample adding test is completed, placing the water-permeable sheet on the test paper, and adding the secondary sample adding agent in the container to the water-permeable sheet, so that the secondary sample adding agent in the container is mixed with another secondary sample adding agent in the water-permeable sheet and then released to the test paper.

20. The detection method according to claim 19, wherein: The secondary sample addition agent is a sensitizer, which performs sensitization and color development on the detection result of the primary sample addition.

21. The detection method as claimed in claim 19, further comprising providing one or more guide grooves on the periphery of the abutment top to guide the secondary sample addition agent to the test paper.

22. The detection method according to claim 19, further comprising using a sealing member to movably seal the opening member to a cylindrical container having an open end.

23. The detection method according to claim 19, further comprising applying pressure to a sealed container having a flexible shell to drive the opening component to move so that the abutting top portion breaks through the outflow wall.

24. The detection method as described in claim 19, wherein the detection color development area includes a quality control color development area and a detection result color development area, and the method further includes driving the opening component to move for secondary sampling when the quality control color development area develops color normally and / or the color significance parameter of the detection result color development area is lower than a predetermined threshold.

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