Triple inactivated vaccine used for porcine circovirus-3, porcine parvovirus and swine influenza and preparation method thereof

A technology for porcine circovirus and parvovirus, applied in biochemical equipment and methods, vaccines, viruses, etc., can solve the problems of high cost, poor prevention and control effect, and large immunization workload, and achieve high cost and convenient immunization. , the effect of high titer content

CN109550045AActive Publication Date: 2019-04-02哈药集团生物疫苗有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2019-04-02

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Abstract

The invention discloses a triple inactivated vaccine used for porcine circovirus-3, porcine parvovirus and swine influenza and a preparation method thereof. The present invention first discloses the triple inactivated vaccine for the prevention and treatment of porcine circovirus syndrome, porcine parvovirus disease and swine influenza. The vaccine comprises: an inactivated antigenic solution of porcine circovirus-3 Cap protein, an inactivated antigenic solution of porcine parvovirus, an antigenic solution of porcine influenza virus and a vaccine adjuvant. The invention further discloses a method for preparing the triple inactivated vaccine, and the method comprises: mixing inactivated antigenic solutions together, uniformly mixing the mixed solutions with the vaccine adjuvant, and performing emulsification. The preparation method of the triple inactivated vaccine of the invention is simple, the vaccine has a high titer content and is convenient and quick for immunization. The triple vaccine provided by the invention has better safety, achieves "one shot with three preventions", avoids adverse reactions caused by multiple vaccination, reduces breeding cost, reduces the immunizationtimes, and can also effectively reduce the stress response of animals and improve the welfare treatment for animals.
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Description

technical field

[0001] The present invention relates to the triple inactivated vaccine of circovirus type 3, porcine parvovirus and swine influenza (H3N2 subtype), and the present invention further relates to the preparation method of the triple inactivated vaccine and its application in the prevention and treatment of PMWS syndrome, porcine parvovirus disease and The application in swine flu belongs to the field of preparation and application of triple inactivated vaccine of porcine circovirus type 3, porcine parvovirus and swine flu. Background technique

[0002] Porcine circovirus (PCV) belongs to the genus Circovirus of the Circoviridae family. It is a small, non-encapsulated, covalently closed, circular, single-stranded DNA virus with an average particle diameter of 17nm. Smallest animal virus ever discovered. Currently, PCV can be divided into 3 genotypes, namely PCV-1, PCV-2 and PCV3. PCV-1 is a non-pathogenic PCV, and both PCV-2 and PCV-3 are pathogenic to pigs and...

Examples

Embodiment 1

[0028] Embodiment 1 expresses the preparation of PCV3 type Cap protein recombinant baculovirus vector, PPV and S IV (H3N2 subtype) triple inactivated vaccine

[0029] 1 Obtaining Antigen Solution of Porcine Circovirus Type 3 Recombinant Baculovirus

[0030] 1.1 Construction of recombinant baculovirus vector expressing PCV3 type Cap protein

[0031] Use the primers in the following table 1 to amplify the Cap protein gene (ORF2) from the PCV3 strain DNA extracted from the suspected porcine circovirus disease material in Jilin, and after recovery, digest the recovery product with BamH I and Xho I respectively , and then ligated with the same digested plasmid pOET1, the obtained recombinant plasmid was identified by restriction enzyme digestion and PCR, and it was determined that the recombinant plasmid pOET1-ORF2-ChS-1 was obtained. Sf9 insect cells (Invitrogen) were co-transfected with insect virus DNA (flashBACPrime) from Oxford Expression Technologies, UK, and transfer plasmi...

Embodiment 2 3

[0074] The preparation of embodiment 2 triple inactivated vaccines

[0075] The preparation of porcine circovirus type 3 recombinant baculovirus antigen solution, porcine parvovirus L strain antigen solution and swine influenza H3N2 subtype HLJ strain antigen solution was prepared according to the method in Example 1, and the pig circovirus that passed the inactivation test was Virus type 3 recombinant baculovirus ChS-1 strain Cap protein antigen solution, PPV L strain antigen solution and SIV H3N2 subtype HLJ strain antigen solution were prepared in a ratio of 1:1:1 to prepare a triple inactivated vaccine, and the rest were mixed with Example 1 is the same.

Embodiment 3 3

[0076] The preparation of embodiment 3 triple inactivated vaccines

[0077] The preparation of porcine circovirus type 3 recombinant baculovirus antigen solution, porcine parvovirus L strain antigen solution and swine influenza H3N2 subtype HLJ strain antigen solution was prepared according to the method in Example 1, and the pig circovirus that passed the inactivation test was Virus type 3 recombinant baculovirus ChS-1 strain Cap protein antigen solution, PPV L strain antigen solution and SIV H3N2 subtype HLJ strain antigen solution were prepared in a ratio of 2:1:1 to prepare a triple inactivated vaccine, and the rest were mixed with Example 1 is the same.