A lab-on-a-chip detection device

Abstract

The on-chip lab detection device disclosed in the present invention includes a shell, a traction device, and a constant temperature module, a liquid squeezing module, and a light sensing module arranged inside the shell; the traction device is used to pull the on-chip lab to move inside the shell; the constant temperature module includes a thermostat and a first pump head, the thermostat is used to provide a constant reaction temperature for the reaction zone of the on-chip lab, and the first pump head is used to drive the first pump body to work and drive the liquid to circulate in the reaction zone; the liquid squeezing module includes an unsealing device and an extrusion device, the unsealing device is used to open the valve of the reagent package of the on-chip lab, and the extrusion device squeezes the reagent package; the light sensing module includes a photodetector, and the photodetector is used to obtain the light intensity data of the reaction zone. In this way, the on-chip lab detection device provided by the present invention can complete the entire process of traditional laboratory detection, and the tester only needs to add samples to the on-chip lab, which simplifies the tester's operation and improves the detection convenience of the experiment.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a laboratory-on-a-chip detection device. Background Art

[0002] Lab-on-a-chip technology integrates the basic operating units of sample preparation, reaction, separation, and detection in the biological, chemical, and medical analysis process onto a micron-scale chip to automatically complete the entire analysis process. Due to its huge potential in the fields of biology, chemistry, and medicine, it has developed into a new research field that intersects biology, chemistry, medicine, fluids, electronics, materials, and mechanics.

[0003] Currently, traditional laboratories need to use multiple instruments to detect and analyze samples. Users need to switch instruments multiple times to complete the entire testing process, which increases the complexity of the test.

[0004] It can be seen that the detection operation of traditional laboratory testing equipment is complicated and not very convenient. Summary of the invention

[0005] In view of this, the present invention proposes a lab-on-a-chip detection device to solve the above technical problems.

[0006] Firstly, to achieve the above-mentioned purpose, the present invention proposes a laboratory-on-a-chip detection device, which includes a housing, a traction device, and a constant temperature module, a liquid squeezing module, and a light sensing module arranged inside the housing;

[0007] The first end of the shell is provided with an opening, and the opening is used for inserting a laboratory-on-a-chip into or removing it from the shell;

[0008] The traction device is used for traction of the on-chip lab to move inside the housing;

[0009] The constant temperature module includes a thermostat and a first pump head. When the on-chip lab moves to the position of the constant temperature module, the thermostat covers the reaction area of ​​the on-chip lab to provide a constant reaction temperature for the reaction area. The first pump head is connected to the first pump body of the on-chip lab and drives the first pump body to work, driving the liquid in the reaction area to circulate in the reaction area.

[0010] The liquid squeezing module includes an unsealing device and an extruding device. When the on-chip laboratory moves to the position of the liquid squeezing module, the unsealing device opens a valve of a reagent pack of the on-chip laboratory, and the extruding device squeezes the reagent pack, so that the liquid in the reagent pack enters the flow channel of the on-chip laboratory;

[0011] The light sensing module includes a photodetector. When the on-chip lab moves to the position of the light sensing module, the photodetector is aligned with the reaction area of ​​the on-chip lab and obtains light intensity data of the reaction area.

[0012] Optionally, the lab-on-a-chip detection device further includes a pre-processing module;

[0013] The pre-processing module includes a blocking device and a second pump head. When the on-chip laboratory moves to the position of the pre-processing module, the blocking device blocks the inlet of the capillary blood collection tube of the on-chip laboratory. The second pump head is connected to the second pump body of the on-chip laboratory and drives the second pump body to supply gas to the capillary blood collection tube of the on-chip laboratory.

[0014] Optionally, the pre-processing module further comprises a first liquid sensor, and when the on-chip lab moves to the position of the pre-processing module, the first liquid sensor is located at the outlet position of the reaction zone, and is used to detect whether the liquid reaches the outlet position of the reaction zone;

[0015] When the liquid reaches the outlet position of the reaction zone, the second pump head stops working and is separated from the second pump body.

[0016] Optionally, the light sensing module further includes a darkroom disposed between the photodetector and the traction device, and when the on-chip laboratory moves to the position of the light sensing module, the darkroom is located between the photodetector and the on-chip laboratory.

[0017] Optionally, the darkroom is an annular darkroom, and when the on-chip laboratory moves to the position of the light sensing module, the darkroom and the on-chip laboratory are interference-fitted.

[0018] Optionally, a compartment cover is further provided at the first end of the shell, and the compartment cover is connected to the shell via a connecting device, and can cover or expose the opening when the compartment cover and the shell are relatively rotated.

[0019] Optionally, the connecting device includes a connecting rod mechanism and a second elastic device, and the connecting rod mechanism is pulled to move the compartment cover in a direction away from the opening, and the second elastic device is stretched. After the on-chip lab completely enters the shell, the elastic restoring force of the second elastic device drives the compartment cover to move in a direction close to the opening, thereby covering the opening;

[0020] When the on-chip laboratory moves from the inside of the shell to the opening position, it supports the connecting rod mechanism and drives the compartment cover to move in a direction away from the opening. After the on-chip laboratory is pulled out, the elastic restoring force of the second elastic device drives the compartment cover to move in a direction close to the opening, thereby covering the opening.

[0021] Optionally, the constant temperature module further comprises a first elastic device connected to the thermostat, and when the thermostat contacts the on-chip lab, the first elastic device is compressed and the elastic restoring force of the first elastic device enables the thermostat to contact closely with the on-chip lab.

[0022] Optionally, the constant temperature module further comprises a first positioning sensor, and when the first positioning sensor is aligned with the first grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the constant temperature module;

[0023] The squeezing module further comprises a second positioning sensor, and when the second positioning sensor is aligned with the second grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the squeezing module;

[0024] The light sensing module further comprises a third positioning sensor, and when the third positioning sensor is aligned with a third grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the light sensing module;

[0025] The pre-processing module further includes a fourth positioning sensor. When the fourth positioning sensor is aligned with a fourth grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the pre-processing module.

[0026] Optionally, the optical sensing module outer shell is provided with a shock-absorbing sleeve.

[0027] Compared with the prior art, the on-chip lab detection device proposed in the present invention includes a shell, a traction device, and a constant temperature module, a liquid squeezing module, and a light sensing module arranged inside the shell; the first end of the shell is provided with an opening, and the opening is used for inserting or removing an on-chip lab into or out of the shell; the traction device is used to pull the on-chip lab to move inside the shell; the constant temperature module includes a thermostat and a first pump head, when the on-chip lab moves to the position of the constant temperature module, the thermostat covers the reaction zone of the on-chip lab, and is used to provide a constant reaction temperature for the reaction zone, and the first pump head and the on-chip lab are connected to each other. The first pump body is connected to and drives the first pump body to work, driving the liquid in the reaction zone to circulate in the reaction zone; the squeezing module includes an unsealing device and an extruding device. When the on-chip laboratory moves to the position of the squeezing module, the unsealing device opens the valve of a reagent pack of the on-chip laboratory, and the extruding device squeezes the reagent pack, and the liquid in the reagent pack enters the flow channel of the on-chip laboratory; the light sensing module includes a photodetector. When the on-chip laboratory moves to the position of the light sensing module, the photodetector is aligned with the reaction zone of the on-chip laboratory and obtains the light intensity data of the reaction zone. In this way, the on-chip laboratory detection device provided by the present invention can complete the entire process of traditional laboratory detection. The detector only needs to add samples to the on-chip laboratory, which simplifies the operation of the detector, improves the detection convenience of the experiment, and facilitates detection anytime and anywhere. In addition, the on-chip laboratory detection device provided by the present invention drives the built-in pump body of the on-chip laboratory to automatically transfer liquid, effectively reducing the human error caused by manual transfer, and can effectively improve the detection accuracy of the on-chip laboratory detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is one of the connection diagrams of the on-chip lab detection device provided by an embodiment of the present invention;

[0029] Figure 2 is a structural schematic diagram of a constant temperature module provided by an embodiment of the present invention;

[0030] Figure 3 is a structural schematic diagram of a liquid squeezing module provided by an embodiment of the present invention;

[0031] Figure 4 is a structural schematic diagram of a light sensing module provided by an embodiment of the present invention;

[0032] Figure 5 is a schematic structural diagram of a lab-on-a-chip provided by an embodiment of the present invention;

[0033] Figure 6 This is the second connection diagram of the on-chip lab detection device provided by the embodiment of the present invention;

[0034] Figure 7 It is a structural schematic diagram of a pre-processing module provided in an embodiment of the present invention.

[0035] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0036] It should be understood that the specific embodiments described herein are only used to explain the present invention, and are not used to limit the present invention.

[0037] See also Figure 1 , Figure 1 FIG. 1 is one of the connection diagrams of the on-chip lab detection device provided by an embodiment of the present invention. Figure 1 As shown, the lab-on-a-chip detection device 100 includes a housing 10 , a traction device 20 , and a constant temperature module 30 , a liquid squeezing module 40 , and a light sensing module 50 disposed inside the housing 10 .

[0038] The housing 10 has an opening 11 at a first end, and the opening 11 is used for inserting a laboratory-on-a-chip into or removing it from the housing 10 .

[0039] The traction device 20 is used to pull the lab-on-a-chip to move inside the housing 10. The traction device 20 may include a motor and a conveyor belt, and the conveyor belt moves under the drive of the motor.

[0040] Please also read Figure 2 , Figure 2 is a schematic diagram of the structure of a constant temperature module provided by an embodiment of the present invention, such as Figure 2 As shown, the constant temperature module 30 includes a thermostat 31 and a first pump head 32. When the on-chip laboratory moves to the position of the constant temperature module 30, the thermostat 31 covers the reaction area of ​​the on-chip laboratory to provide a constant reaction temperature for the reaction area. The first pump head 32 is connected to the first pump body of the on-chip laboratory and drives the first pump body to work, driving the liquid in the reaction area to circulate in the reaction area.

[0041] Please also read Figure 3 , Figure 3 is a schematic diagram of the structure of a liquid squeezing module provided by an embodiment of the present invention, such as Figure 3 As shown, the liquid squeezing module 40 includes an unsealing device 41 and an extruding device 42. When the on-chip laboratory moves to the position of the liquid squeezing module 40, the unsealing device 41 opens the valve of a reagent pack of the on-chip laboratory, and the extruding device 42 squeezes the reagent pack, and the liquid in the reagent pack enters the flow channel of the on-chip laboratory.

[0042] Please also read Figure 4 , Figure 4 is a schematic diagram of the structure of a light sensing module provided by an embodiment of the present invention, such as Figure 4 As shown, the light sensing module 50 includes a photodetector 51. When the on-chip lab moves to the position of the light sensing module 50, the photodetector 51 is aligned with the reaction area of ​​the on-chip lab and obtains light intensity data of the reaction area.

[0043] In an embodiment of the present invention, the on-chip lab detection device includes a shell, a traction device, and a constant temperature module, a liquid squeezing module, and a light sensing module arranged inside the shell; the first end of the shell is provided with an opening, and the opening is used for inserting or removing a on-chip lab into or out of the shell; the traction device is used to pull the on-chip lab to move inside the shell; the constant temperature module includes a thermostat and a first pump head, when the on-chip lab moves to the position of the constant temperature module, the thermostat covers the reaction area of ​​the on-chip lab, and is used to provide a constant reaction temperature for the reaction area, and the first pump head and the first pump head of the on-chip lab are connected to each other. The pump body is connected to and drives the first pump body to work, driving the liquid in the reaction zone to circulate in the reaction zone; the squeezing module includes an unsealing device and an extruding device. When the on-chip laboratory moves to the position of the squeezing module, the unsealing device opens the valve of a reagent pack of the on-chip laboratory, and the extruding device squeezes the reagent pack, and the liquid in the reagent pack enters the flow channel of the on-chip laboratory; the light sensing module includes a photodetector. When the on-chip laboratory moves to the position of the light sensing module, the photodetector is aligned with the reaction zone of the on-chip laboratory and obtains the light intensity data of the reaction zone. In this way, the on-chip laboratory detection device provided by the present invention can complete the entire process of on-chip laboratory detection. The detector only needs to add samples to the on-chip laboratory, which simplifies the detector's operation and improves the convenience of on-chip laboratory detection. In addition, the on-chip laboratory detection device provided by the present invention drives the built-in pump body of the on-chip laboratory to automatically transfer liquid, effectively reducing the human error caused by manual transfer, and can effectively improve the detection accuracy of the on-chip laboratory detection device.

[0044] The following describes the on-chip lab detection device of the present invention in detail in conjunction with the on-chip lab. Figure 5 , Figure 5 is a schematic diagram of the structure of a lab-on-a-chip provided by an embodiment of the present invention, such as Figure 5 As shown, the on-chip laboratory 200 includes a sample inlet 201, a reagent area 202, a reaction area 203 and a waste liquid pool 204. The inlet of the reaction area 203 is connected to the sample inlet 201 and the reagent area 202 through a flow channel 205, and the outlet of the reaction area 203 is connected to the waste liquid pool 204 through a flow channel 205.

[0045] A filter 206 is provided between the sample inlet 201 and the inlet of the reaction zone 203. The inlet and outlet of the reaction zone 203 are respectively connected to the first pump body 207 through the flow channel 205, and the first pump body 207 is provided with a first grating hole 2071. The reagent zone 202 includes at least four reagent packs 2021, each of which is provided with a second grating hole 2022, and each reagent pack 2021 is provided with a valve 2023 between the inlet of the reaction zone 203 and the reagent pack 2021.

[0046] The reaction zone 203 includes at least two reactors 2031 , each reactor 2031 is provided with a third grating hole 2032 , and each reactor 2031 is connected in sequence, that is, the at least two reactors 2031 are connected in series.

[0047] A first separator 208 may be provided between the filter 206 and the inlet of the reaction zone 203, and the first separator 208 is used to remove bubbles doped in the sample. A second pump body 209 may be provided between the sample inlet 201 and the filter 206, and a fourth grating hole 2091 is provided at the position of the second pump body 209.

[0048] The on-chip lab 200 may further include a cover 210, and the cover 210 is used to close the sample inlet 201 after the sample is injected into the sample inlet 201. A second separator 211 may be provided at the outlet of the waste liquid pool 204, and the second separator 211 is used to discharge bubbles in the waste liquid pool 204. In some embodiments, the on-chip lab 200 further includes a capillary blood collection tube 212, and the sample inlet 201 is a blood collection port of the capillary blood collection tube 212. A first check valve 213 may be provided between two adjacent reagent packs 2021, and the first check valve 213 can prevent the liquid in the reagent pack 2021 from flowing into the adjacent empty pack when one of the reagent packs 2021 is squeezed, thereby effectively reducing the waste of liquid. A second check valve 213 may be provided between the filter 206 and the inlet of the reaction zone 203. The second check valve 213 prevents the liquid from bypassing the second pump body 209 during the sample injection process and / or when the liquid in the reagent pack 2021 is perfused into the reaction zone 203. A third check valve 213 may be provided between the first pump body 207 and the inlet of the reaction zone 203. The third check valve 213 is used to prevent the liquid from flowing back to the first pump body 207 when the first pump body 207 is working. In this way, the liquid in the flow channel 205 can be effectively prevented from flowing back by providing the check valve 213.

[0049] When in use, the lab-on-chip 200 can be inserted into the housing 10 from the opening 11, and the traction device 20 drives the lab-on-chip 200 to move inside the housing 10. It can be understood that in the embodiment of the present invention, the lab-on-chip detection device 100 also includes a processor (not shown), and the other components of the lab-on-chip detection device 100 are electrically connected to the processor, and the control of the other components can be achieved by writing a program in the processor. For example, the distance and direction of the lab-on-chip 200 driven by the traction device 20 can be controlled by the processor, which is not described in detail in the embodiment of the present invention.

[0050] When the traction device 20 drives the on-chip lab 200 to move to the position of the constant temperature module 30, the thermostat 31 covers the reaction area 203 of the on-chip lab 200, and the thermostat 31 is used to provide a constant reaction temperature for the reaction area 203. Usually, the on-chip lab 200 is stored in an environment of 2-8 degrees Celsius. When testing, it is necessary to simulate the body temperature environment of the human body. The thermostat 31 is used to heat the on-chip lab 200 within the normal body temperature range of the human body (for example, 37 degrees Celsius) and maintain it. Specifically, the thermostat 31 may include a heating plate and a temperature detection device, and the processor controls the heating plate to start or stop heating according to the temperature detected by the temperature detection device. When the traction device 20 drives the on-chip lab 200 to move to the position of the constant temperature module 30, the first pump head 32 is connected to the first pump body 207 of the on-chip lab 200, and drives the first pump body 207 to work, so that the first pump body 207 and the reaction area 203 form a loop, thereby driving the liquid in the reaction area 203 to circulate in the reaction area 203, and achieving full contact between the liquid and the reaction area 203. In this way, the constant temperature module 30 can make the liquid fully contact with the reaction area 203 and provide an accurate reaction temperature, thereby improving the detection accuracy.

[0051] When the traction device 20 drives the on-chip lab 200 to move to the position of the liquid squeezing module 40, the unsealing device 41 presses one of the valves 2023 to open it, and the squeezing device 42 squeezes the reagent package 2021 corresponding to the valve 2023, so that the liquid in the reagent package 2021 enters the flow channel 205 of the on-chip lab 200. In this way, the liquid flow rate can be accurately controlled by the squeezing device 42.

[0052] When the traction device 20 drives the on-chip laboratory 200 to move to the position of the light sensing module 50, the photodetector 51 is aimed at the reaction area 203 of the on-chip laboratory 200, obtains the light intensity data of the reaction area 203, and sends the obtained light intensity data to the processor, so that the processor can analyze the detection results according to the light intensity data.

[0053] The traction device 20 can be a hook traction, and accordingly, the on-chip laboratory 200 is provided with a traction buckle that matches the traction device 20 .

[0054] It should be noted that, for the convenience of description, in the embodiment of the present invention, each module position in the drawings is marked with an on-chip lab, wherein only one position is marked with a solid line, and the rest are marked with dotted lines. That is, in a test, the on-chip lab 200 can only be in one position at the same time, and the drawings are only for the convenience of description.

[0055] Optionally, the lab-on-a-chip detection device further includes a pre-processing module;

[0056] The pre-processing module includes a blocking device and a second pump head. When the on-chip laboratory moves to the position of the pre-processing module, the blocking device blocks the inlet of the capillary blood collection tube of the on-chip laboratory. The second pump head is connected to the second pump body of the on-chip laboratory and drives the second pump body to supply gas to the capillary blood collection tube of the on-chip laboratory.

[0057] See also Figure 6 , Figure 6 FIG. 2 is a second structural diagram of a lab-on-a-chip detection device provided by an embodiment of the present invention. Figure 6 As shown, the on-chip lab detection device 100 also includes a pre-processing module 60 near the first end of the housing 10, please refer to Figure 7 , Figure 7 is a schematic diagram of the structure of the pre-processing module 60 provided in an embodiment of the present invention, such as Figure 7As shown, the pre-processing module 60 includes a blocking device 61 and a second pump head 62. When the traction device 20 drives the on-chip lab 200 to move to the position of the pre-processing module 60, the blocking device 61 blocks the inlet of the capillary blood collection tube 212 of the on-chip lab 200. The second pump head 62 is connected to the second pump body 209 of the on-chip lab 200 and drives the second pump body 209 to supply gas to the capillary blood collection tube 212 of the on-chip lab 200. In this way, the inlet of the capillary blood collection tube 212 can be sealed to prevent the second pump body 209 from pumping out the sample from the inlet of the capillary blood collection tube 212 when working. It should be noted that when the on-chip lab 200 does not include the cover body 210, the blocking device 61 is used to seal the inlet of the capillary blood collection tube 212. When the on-chip lab 200 includes the cover body 210, the blocking device 61 is used to reinforce the sealing effect of the inlet of the capillary blood collection tube 212.

[0058] In this embodiment, the opening 11 can be opened at an end away from the pre-processing module 60, so that when the on-chip laboratory 200 is inserted into the shell from the opening 11, the traction device 20 pulls the on-chip laboratory 200 to move to the pre-processing module 60, so that the blocking device 61 blocks the entrance of the capillary blood collection tube 212.

[0059] Optionally, the pre-processing module further comprises a first liquid sensor, and when the on-chip lab moves to the position of the pre-processing module, the first liquid sensor is located at the outlet position of the reaction zone, and is used to detect whether the liquid reaches the outlet position of the reaction zone;

[0060] When the liquid reaches the outlet position of the reaction zone, the second pump head stops working and is separated from the second pump body.

[0061] In this embodiment, the pre-processing module 60 further includes a first liquid sensor 63. When the on-chip lab 200 moves to the position of the pre-processing module 60, the first liquid sensor 63 is located at the outlet of the reaction zone 203, and is used to detect whether the liquid has reached the outlet of the reaction zone 203. When the liquid reaches the outlet of the reaction zone 203, it means that the liquid has filled the reaction zone 203 and no further liquid flow is required, and the second pump head 62 stops working and is separated from the second pump body 209.

[0062] In some embodiments of the present invention, the pre-processor module 60 may further include a second liquid sensor 64. When the on-chip lab 200 moves to the position of the pre-processor module 60, the second liquid sensor 64 is located at the entrance of the reaction area 203 to detect whether the liquid reaches the entrance of the reaction area 203. In this way, it is convenient for the user to know the amount of sample injected in time.

[0063] Optionally, the light sensing module further includes a darkroom disposed between the photodetector and the traction device, and when the on-chip laboratory moves to the position of the light sensing module, the darkroom is located between the photodetector and the on-chip laboratory.

[0064] Since ambient light can easily have a significant impact on the detection results, in this embodiment, the light sensing module 50 further includes a darkroom 52 disposed between the photodetector 51 and the traction device 20. When the on-chip laboratory 200 moves to the position of the light sensing module 50, the darkroom 52 is located between the photodetector 51 and the on-chip laboratory 200, and is used to shield the on-chip laboratory 200 from ambient light, thereby reducing the impact of ambient light on the detection results, thereby improving the detection accuracy.

[0065] Optionally, the darkroom is an annular darkroom, and when the on-chip laboratory moves to the position of the light sensing module, the darkroom and the on-chip laboratory are interference-fitted.

[0066] In this embodiment, the darkroom 52 is an annular darkroom, such as an “O” shape. When the on-chip lab 200 moves to the position of the light sensing module 50 , the darkroom 52 and the on-chip lab 200 are interference-fitted.

[0067] Optionally, a compartment cover is further provided at the first end of the shell, and the compartment cover is connected to the shell via a connecting device, and can cover or expose the opening when the compartment cover and the shell are relatively rotated.

[0068] In this embodiment, a compartment cover 12 is further provided at the first end of the housing 10, and the compartment cover 12 is connected to the housing 10 via a connecting device 13, and can cover or expose the opening 11 when the compartment cover 12 and the housing 10 rotate relative to each other. In this way, when the opening 11 is exposed, it is convenient to insert or withdraw the on-chip laboratory, and when the opening 11 is covered by the compartment cover 12, it can effectively block ambient light, thereby improving the detection accuracy.

[0069] Optionally, the connecting device includes a connecting rod mechanism and a second elastic device (not shown in the figure), and when the connecting rod mechanism is pulled, the bin cover moves in a direction away from the opening, and the second elastic device is stretched. After the on-chip laboratory completely enters the shell, the elastic restoring force of the second elastic device drives the bin cover to move in a direction close to the opening, thereby covering the opening; when the on-chip laboratory moves from the inside of the shell to the opening position, it supports the connecting rod mechanism and drives the bin cover to move in a direction away from the opening. After the on-chip laboratory is pulled out, the elastic restoring force of the second elastic device drives the bin cover to move in a direction close to the opening, thereby covering the opening.

[0070] Optionally, the constant temperature module further comprises a first elastic device connected to the thermostat, and when the thermostat contacts the on-chip lab, the first elastic device is compressed and the elastic restoring force of the first elastic device enables the thermostat to contact closely with the on-chip lab.

[0071] In this embodiment, the constant temperature module 30 also includes a first elastic device 34 connected to the thermostat 31. When the thermostat 31 contacts the on-chip laboratory 200, the first elastic device 34 is compressed, and the elastic restoring force of the first elastic device 34 enables the thermostat 31 to be in close contact with the on-chip laboratory 200, thereby improving the constant temperature effect of the on-chip laboratory.

[0072] Optionally, the constant temperature module further comprises a first positioning sensor, and when the first positioning sensor is aligned with the first grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the constant temperature module;

[0073] The squeezing module further comprises a second positioning sensor, and when the second positioning sensor is aligned with the second grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the squeezing module;

[0074] The light sensing module further comprises a third positioning sensor, and when the third positioning sensor is aligned with a third grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the light sensing module;

[0075] The pre-processing module further includes a fourth positioning sensor. When the fourth positioning sensor is aligned with a fourth grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the pre-processing module.

[0076] In this embodiment, each module of the lab-on-a-chip detection device 100 is provided with a positioning sensor, and the positioning sensor cooperates with the grating holes of the lab-on-a-chip 200 to achieve accurate positioning of the lab-on-a-chip 200 .

[0077] Specifically, the constant temperature module 30 includes a first positioning sensor 33 . When the first positioning sensor 33 is aligned with the first grating hole 2071 of the on-chip lab 200 , it is determined that the on-chip lab 200 moves to the position of the constant temperature module 30 .

[0078] The squeezing module 40 includes a second positioning sensor 43 . When the second positioning sensor 43 is aligned with a second grating hole 2022 of the on-chip lab 200 , it is determined that the on-chip lab 200 moves to the position of the squeezing module 40 .

[0079] The light sensing module 50 includes a third positioning sensor 53 . When the third positioning sensor 53 is aligned with a third grating hole 2032 of the on-chip lab 200 , it is determined that the on-chip lab 200 moves to the position of the light sensing module 50 .

[0080] The pre-processing module 60 includes a fourth positioning sensor 65 . When the fourth positioning sensor 65 is aligned with the fourth grating hole 2091 of the lab-on-chip 200 , it is determined that the lab-on-chip 200 moves to the position of the pre-processing module 60 .

[0081] Optionally, the optical sensing module outer shell is provided with a shock-absorbing sleeve.

[0082] In this embodiment, a shock-absorbing sleeve (not shown) may be provided outside the light sensing module 50 . The shock-absorbing sleeve may be made of an elastic material. The light sensing module 50 may be protected by the shock-absorbing sleeve.

[0083] In the prior art, adding samples to the reaction area requires manual operation by the user, which is prone to human error. The on-chip laboratory detection device provided by the embodiment of the present invention drives the pump body built into the on-chip laboratory to automatically transfer liquid through the pump head, ensuring the accuracy and consistency of liquid transfer, and can improve the precision and repeatability of microfluidic products.

[0084] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A lab-on-a-chip detection device, characterized in that: The on-chip laboratory detection device includes a housing, a traction device, and a constant temperature module, a liquid squeezing module, and a light sensing module arranged inside the housing; The first end of the shell is provided with an opening, and the opening is used for inserting a laboratory-on-a-chip into or removing it from the shell; The traction device is used for traction of the on-chip lab to move inside the housing; The constant temperature module includes a thermostat and a first pump head. When the on-chip lab moves to the position of the constant temperature module, the thermostat covers the reaction area of ​​the on-chip lab to provide a constant reaction temperature for the reaction area. The first pump head is connected to the first pump body of the on-chip lab and drives the first pump body to work, driving the liquid in the reaction area to circulate in the reaction area. The liquid squeezing module includes an unsealing device and an extruding device. When the on-chip laboratory moves to the position of the liquid squeezing module, the unsealing device opens a valve of a reagent pack of the on-chip laboratory, and the extruding device squeezes the reagent pack, so that the liquid in the reagent pack enters the flow channel of the on-chip laboratory; The light sensing module includes a photodetector. When the on-chip lab moves to the position of the light sensing module, the photodetector is aligned with the reaction area of ​​the on-chip lab and obtains light intensity data of the reaction area. Wherein, the constant temperature module further comprises a first elastic device connected to the thermostat, and when the thermostat contacts the on-chip lab, the first elastic device is compressed and the elastic restoring force of the first elastic device enables the thermostat to contact closely with the on-chip lab; Wherein, a compartment cover is further provided at the first end of the shell, and the compartment cover is connected to the shell through a connecting device, and when the compartment cover and the shell rotate relative to each other, the opening can be covered or exposed; Wherein, the connecting device includes a connecting rod mechanism and a second elastic device, and when the connecting rod mechanism is pulled, the compartment cover moves in a direction away from the opening, and the second elastic device is stretched. After the on-chip lab completely enters the shell, the elastic restoring force of the second elastic device drives the compartment cover to move in a direction close to the opening, thereby covering the opening; When the on-chip laboratory moves from the inside of the shell to the opening position, it supports the connecting rod mechanism and drives the compartment cover to move in a direction away from the opening. After the on-chip laboratory is pulled out, the elastic restoring force of the second elastic device drives the compartment cover to move in a direction close to the opening, thereby covering the opening.

2. The on-chip lab detection device according to claim 1, characterized in that: The lab-on-a-chip detection device also includes a pre-processing module; The pre-processing module includes a blocking device and a second pump head. When the on-chip laboratory moves to the position of the pre-processing module, the blocking device blocks the inlet of the capillary blood collection tube of the on-chip laboratory. The second pump head is connected to the second pump body of the on-chip laboratory and drives the second pump body to supply gas to the capillary blood collection tube of the on-chip laboratory.

3. The on-chip lab detection device according to claim 2, characterized in that: The pre-processing module further comprises a first liquid sensor, which is located at the outlet of the reaction zone when the lab-on-a-chip moves to the pre-processing module, and is used to detect whether the liquid reaches the outlet of the reaction zone; When the liquid reaches the outlet position of the reaction zone, the second pump head stops working and is separated from the second pump body.

4. The on-chip lab detection device according to claim 1, characterized in that: The light sensing module further comprises a darkroom disposed between the photodetector and the traction device. When the on-chip laboratory moves to the position of the light sensing module, the darkroom is located between the photodetector and the on-chip laboratory.

5. The on-chip lab detection device according to claim 4, characterized in that: The darkroom is an annular darkroom. When the on-chip laboratory moves to the position of the light sensing module, the darkroom and the on-chip laboratory are interference-fitted.

6. The on-chip lab detection device according to claim 2, characterized in that: The constant temperature module further comprises a first positioning sensor, and when the first positioning sensor is aligned with the first grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the constant temperature module; The squeezing module further comprises a second positioning sensor, and when the second positioning sensor is aligned with the second grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the squeezing module; The light sensing module further comprises a third positioning sensor, and when the third positioning sensor is aligned with a third grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the light sensing module; The pre-processing module further includes a fourth positioning sensor. When the fourth positioning sensor is aligned with a fourth grating hole of the on-chip lab, it is determined that the on-chip lab moves to the position of the pre-processing module.

7. The lab-on-a-chip detection device according to claim 1, characterized in that: The outer jacket of the light sensing module is provided with a shock absorbing sleeve.

Images