A film making and dyeing machine

By designing a production and dyeing machine containing multiple mechanical mechanisms, the problems of automated and centrifuge tube damage in the existing liquid-based cell production technology are solved, and an efficient and automated liquid-based cell production process is achieved.

CN111366441BActive Publication Date: 2025-02-18EPS BIOTECHNOLOGY (SHENZHEN) CO LTD
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Patent Information

Application Number
CN202010280908.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2020-04-10
Publication Date
2025-02-18
Estimated Expiration
2040-04-10

AI Technical Summary

Technical Problem

The existing liquid-based cell production technology cannot be automated, the production efficiency is low, and the centrifuge tube is easily damaged during the centrifugation process.

Method used

A tableting dyeing machine is designed, including a type frame, an X-axis slip mechanism, a Y-axis slip mechanism, a pipetting mechanism, an injection mechanism, a feeding mechanism, a centrifugal mechanism, a dyeing mechanism and a control device, which is realized through mechanization. Automatically produce the film and control the swing direction and angle of the centrifuge tube during centrifugation.

Benefits of technology

It realizes the automation of liquid-based cell production, improves production efficiency, avoids damage to the centrifuge tube, ensures good centrifugal effect, and has a better user experience.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a slide making and dyeing machine, comprising a type-A frame, an X-axis sliding mechanism, a Y-axis sliding mechanism, a liquid transfer mechanism, an injection mechanism, a feeding mechanism, a centrifugal mechanism, a dyeing mechanism and a control device; the Y-axis sliding mechanism is arranged on the upper part of the type-A frame, and the X-axis sliding mechanism is slidably connected with the Y-axis sliding mechanism; the liquid transfer mechanism is arranged on the left side of the X-axis sliding mechanism and is slidably connected with the X-axis sliding mechanism; the injection mechanism is arranged on the right side of the X-axis sliding mechanism; the feeding mechanism is arranged on the vertical part of the type-A frame and is connected with the liquid transfer mechanism and the injection mechanism; the bottom of the type-A frame is set as a working platform, and the centrifugal mechanism, the dyeing mechanism and the control device are arranged on the working platform in sequence from left to right. The liquid transfer mechanism and the injection mechanism of the invention can accurately move to the required station, and the injection liquid and the specimen transfer are completed by mechanization, which saves labor costs, realizes automatic slide making, and has high slide making efficiency.
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Description

Technical Field

[0001] The invention relates to the technical field of liquid-based cell slide making, in particular to a slide making and staining machine. Background Art

[0002] At present, in clinical medicine, the presence of pathological changes in the human body is often determined by observing the cell preparations of various tissues. With the rapid development of science and technology and social economy, the development of pathological analysis technology has been greatly promoted, making the second-generation liquid-based cell preparation technology represented by sedimentation liquid-based cell preparation technology more and more widely used in the preparation of cytopathology specimens.

[0003] In the prior art, the production process of liquid-based cell slides includes: sampling, centrifugation, smearing and staining. The specimen transfer and injection of the liquid required for the slides need to be completed manually, which cannot be automated and has low production efficiency. Based on this, the present invention was developed. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a film making and dyeing machine.

[0005] The technical solution of the present invention is as follows: a slide dyeing machine, comprising: a U-shaped frame, an X-axis sliding mechanism, a Y-axis sliding mechanism, a liquid transfer mechanism, an injection mechanism, a feeding mechanism matched with the liquid transfer mechanism and the injection mechanism, a centrifugal mechanism, a dyeing mechanism and a control device; the Y-axis sliding mechanism is arranged on the upper part of the U-shaped frame, the X-axis sliding mechanism is slidably connected with the Y-axis sliding mechanism, and can slide left and right along the Y-axis direction of the Y-axis sliding mechanism; the liquid transfer mechanism is arranged on the left side of the X-axis sliding mechanism, and slides with the X-axis sliding mechanism The X-axis sliding mechanism is connected to the X-axis sliding mechanism and can slide forward and backward along the X-axis direction of the X-axis sliding mechanism; the injection mechanism is arranged on the right side of the X-axis sliding mechanism; the feeding mechanism is arranged on the vertical part of the E-shaped frame and is connected with the pipetting mechanism and the injection mechanism; the bottom of the E-shaped frame is set as a working platform, and the centrifugal mechanism, the dyeing mechanism and the control device are arranged on the working platform in sequence from left to right; the control device is electrically connected to the X-axis sliding mechanism, the Y-axis sliding mechanism, the pipetting mechanism, the injection mechanism, the feeding mechanism and the centrifugal mechanism respectively, and is used to control the operation of the equipment.

[0006] Preferably, the centrifugal mechanism comprises a shell, a power mechanism, a rotating mechanism and a plurality of centrifuge tube racks; the shell comprises a detachable top cover and a bottom frame, the top cover is placed above the bottom frame, and a centrifuge chamber is arranged in the bottom frame; the rotating mechanism is installed below the working platform, and the top of the rotating mechanism passes through the working platform and the bottom frame in turn and is placed in the centrifuge chamber; a plurality of centrifuge tube racks are rotatably installed on the top of the rotating mechanism, each of the centrifuge tube racks is evenly provided with a plurality of tube holes, and a centrifuge tube is placed in each tube hole; the power mechanism is installed below the working platform on one side of the rotating mechanism, and the power mechanism is connected to the rotating mechanism;

[0007] The centrifuge mechanism also includes a waste bucket and a waste liquid tank; the waste bucket and the waste liquid tank are arranged on the working platform from front to back between the shell and the dyeing mechanism, the waste bucket is used to recycle the centrifuge tube, and the waste liquid tank is used to recycle the centrifuge waste liquid.

[0008] Preferably, the rotating mechanism consists of a supporting seat, a rotating bracket and a pressing plate; the supporting seat can be rotatably installed around its own axis and is installed below the working platform, and the bottom of the supporting seat is connected to the power mechanism, and the top of the supporting seat passes through the working platform and the bottom frame in turn and is placed in the centrifugal chamber; the rotating bracket is fixedly installed on the top of the supporting seat, and the rotating bracket is evenly provided with installation bins matching the number of the centrifuge tube racks, and the centrifuge tube rack can be rotatably installed in each of the installation bins; the pressing plate is installed on the rotating bracket above the centrifuge tube rack, and the pressing plate is provided with a convex finger corresponding to the position of each installation bin, and the centrifuge tube rack in each installation bin is provided with a slot corresponding to the position of the convex finger.

[0009] Preferably, the power mechanism comprises a servo motor provided with an encoder, a transmission gear, a synchronous belt and a rotating gear; the rotating gear is fixedly mounted on the bottom of the support seat; the servo motor is mounted on one side of the rotating gear; the transmission gear is fixedly mounted on the output shaft of the servo motor; the synchronous belt is arranged on the transmission gear and the rotating gear;

[0010] The power mechanism further includes two tightening devices; the two tightening devices are respectively installed below the bottom frame on both sides of the synchronous belt to adjust the tightness of the synchronous belt;

[0011] A gravity pressing block is provided at the bottom of each centrifuge tube rack;

[0012] The top cover is provided with a cover plate receiving groove, in which a cover plate is placed; handles are respectively provided on both sides of the cover plate for grabbing the cover plate; and an observation window is also provided on the cover plate between the two handles.

[0013] Preferably, the staining mechanism comprises a plurality of slide staining components and a bracket; the bracket is arranged on a working platform; and a plurality of slide staining components are arranged on the bracket;

[0014] The dyeing mechanism also includes a recovery tank for recovering waste liquid; the recovery tank is arranged on the working platform on the right side of the bracket;

[0015] The dyeing mechanism also includes a recycling bucket for recycling waste materials; the recycling bucket is arranged on the working platform at the rear side of the bracket;

[0016] Each of the slide staining components comprises:

[0017] The dyeing bottle is hollow, with an annular protrusion and a rubber ring at the bottom, and a protrusion extending from each of the two opposite sides of the annular protrusion;

[0018] A carrier plate is provided with grooves arranged in an array, the grooves are used to accommodate glass slides, and each groove has buckle positions on both inner sides, which are used to buckle the annular protrusions on the bottom of the staining bottle;

[0019] Wherein, handles are provided on both sides of the carrier plate for transferring the carrier plate.

[0020] Preferably, the X-axis sliding mechanism includes an X-axis slide rail, a first motor arranged at the end of the X-axis slide rail, and a chain arranged at the output end of the first motor; the chain is arranged on the track of the X-axis slide rail and can slide back and forth along the track of the X-axis slide rail; the pipetting mechanism is arranged on the chain and can slide back and forth on the X-axis slide rail along the chain to move.

[0021] Preferably, the Y-axis sliding mechanism comprises a Y-axis slide rail, a slider slidably connected to the Y-axis slide rail, and a belt transmission mechanism providing power to the slider;

[0022] The belt transmission mechanism comprises a second motor, a driving wheel connected to the output end of the second motor, a driven wheel matched with the driving wheel, and belts arranged on the driving wheel and the driven wheel; the slider is arranged on the belt.

[0023] Preferably, the pipetting mechanism comprises a first pipetting assembly, a first pipetting trough matched with the first pipetting assembly, a second pipetting assembly, a second pipetting trough matched with the second pipetting assembly, a specimen liquid trough, and a connecting plate; the connecting plate is arranged on a chain and can slide forward and backward along the chain on an X-axis slide rail to move; the first pipetting assembly and the second pipetting assembly are arranged on the connecting plate in sequence from front to back, and are respectively used for pipetting;

[0024] The first liquid transfer assembly includes a third motor and a first liquid transfer gun connected to the output end of the third motor; the first liquid transfer gun is also connected to the feeding mechanism through a material tube; the first liquid transfer tank is arranged on the working platform at the front side of the centrifugal mechanism, and the first liquid transfer tank is provided with a plurality of first liquid transfer tubes matched with the first liquid transfer gun;

[0025] The second liquid transfer assembly includes a fourth motor and a second liquid transfer gun connected to the output end of the fourth motor; the second liquid transfer gun is also connected to the feeding mechanism through a material pipe; the second liquid transfer tank is arranged on the working platform at the front side of the waste barrel, and the second liquid transfer tank is provided with a plurality of second liquid transfer tubes matched with the second liquid transfer gun;

[0026] The specimen liquid tank is arranged on the working platform between the second liquid transfer tank and the staining mechanism, and a plurality of specimen liquid tubes are placed in the specimen liquid tank.

[0027] Preferably, the injection mechanism comprises a mounting frame, a fifth motor arranged on the mounting frame, a support plate connected to the output end of the fifth motor, and a plurality of injection assemblies matched with the grooves arranged in an array on the carrier plate; the mounting frame is arranged on the right side of the X-axis slide rail, and each of the injection assemblies is arranged on the support plate corresponding to the position of a row of grooves on the carrier plate;

[0028] Each of the injection assemblies comprises a plurality of groups of dyeing needles and a group of waste liquid needles, and the length of the waste liquid needles is greater than the length of the plurality of groups of dyeing needles; each group of dyeing needles is connected to the feeding mechanism through a material pipe; and the waste liquid needles are used to suck out waste liquid.

[0029] Preferably, the loading mechanism includes several pressure pumps cooperating with the first pipette, the second pipette and several injection components; each of the pressure pumps is externally connected to a liquid storage device, the first pipette is connected to one of the pressure pumps through a material pipe, the second pipette is connected to one of the pressure pumps through a material pipe, and the dyeing needle of each injection component is connected to a corresponding one of the pressure pumps through a material pipe.

[0030] By adopting the above scheme, the present invention has the following beneficial effects:

[0031] 1. The design of the present invention enables the pipetting mechanism and the injection mechanism to accurately move to the required position, and the injection of liquid and the transfer of specimens are completed by mechanization, which saves labor costs, realizes automated film making, and has high film making efficiency;

[0032] 2. In the preferred solution, the swing direction and angle of the centrifuge tube can be controlled during the centrifugation process, effectively avoiding damage to the centrifuge tube due to collision, ensuring the integrity of the centrifuge tube, achieving good centrifugal effect and providing a good user experience;

[0033] 3. The application of the encoder in the preferred solution effectively ensures that the number of revolutions of the servo motor each time is a fixed value, thereby realizing the positioning function of the centrifuge tube rack;

[0034] 4. The application of the tightening device in the preferred solution can effectively adjust the tightness of the synchronous belt and ensure the accuracy of the transmission. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0036] Figure 1 It is a structural schematic diagram of the present invention;

[0037] Figure 2 This is a schematic diagram of the structure of the present invention after removing the top of the frame;

[0038] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0039] Figure 4 It is a schematic diagram of the structure of the X-axis and Y-axis sliding mechanisms and the injection mechanism of the present invention;

[0040] Figure 5 It is a structural schematic diagram of the centrifugal mechanism of the present invention;

[0041] Figure 6 It is a structural schematic diagram of the centrifugal mechanism of the present invention after removing the shell;

[0042] Figure 7 It is a schematic diagram of the structure of the rotating assembly of the centrifugal mechanism of the present invention;

[0043] Figure 8 It is a structural schematic diagram of the dyeing component of the present invention;

[0044] Fig. 9 for Figure 8 Enlarged view of point B in the middle;

[0045] Fig.10 This is a schematic diagram of the top view of the dyeing bottle of the present invention;

[0046] Fig.11 It is a schematic diagram of the bottom viewing angle of the dyeing bottle of the present invention.

[0047] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0048] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0049] Reference Figures 1 to 11 As shown, the present invention provides a slide dyeing machine, comprising a ㄈ-shaped frame 1, an X-axis sliding mechanism 2, a Y-axis sliding mechanism 3, a pipetting mechanism 4, an injection mechanism 5, a feeding mechanism 6 matched with the pipetting mechanism 4 and the injection mechanism 5, a centrifugal mechanism 7, a dyeing mechanism 8 and a control device 9; the Y-axis sliding mechanism 3 is arranged on the upper part of the ㄈ-shaped frame 1, the X-axis sliding mechanism 2 is slidably connected to the Y-axis sliding mechanism 3, and can slide left and right along the Y-axis direction of the Y-axis sliding mechanism 3; the pipetting mechanism 4 is arranged on the left side of the X-axis sliding mechanism 2, and is slidably connected to the X-axis sliding mechanism 2, and can slide back and forth along the X-axis direction of the X-axis sliding mechanism 2. It is arranged on the X-axis sliding mechanism 2, and can then slide left and right along the Y-axis direction of the Y-axis sliding mechanism 3; the injection mechanism 5 is arranged on the right side of the X-axis sliding mechanism 2 corresponding to the position of the dyeing mechanism 8; the feeding mechanism 6 is arranged on the vertical part of the E-type frame 1, and is connected with the pipetting mechanism 4 and the injection mechanism 5 through a material pipe; the bottom of the E-type frame 1 is set as a working platform, and the centrifugal mechanism 7, the dyeing mechanism 8 and the control device 9 are arranged on the working platform in sequence from left to right; the control device 9 is electrically connected to the X-axis sliding mechanism 2, the Y-axis sliding mechanism 3, the pipetting mechanism 4, the injection mechanism 5, the feeding mechanism 6 and the centrifugal mechanism 7, respectively, for controlling the operation of the equipment.

[0050] The centrifugal mechanism 7 includes a shell 71, a power mechanism 72, a rotating mechanism 73 and a plurality of centrifuge tube racks 74; the shell 71 includes a detachable top cover 711 and a bottom frame 712, the top cover 711 is placed above the bottom frame 712, and a centrifuge chamber is arranged in the bottom frame 712; the rotating mechanism 73 is installed below the working platform, and the top of the rotating mechanism 73 passes through the working platform and the bottom frame 712 in turn and is placed in the centrifuge chamber; a plurality of centrifuge tube racks 74 are rotatably installed on the top of the rotating mechanism 73, each of the centrifuge tube racks 74 is evenly provided with a plurality of tube holes, and a centrifuge tube is placed in each tube hole; the power mechanism 72 is installed below the working platform on one side of the rotating mechanism 73, and the power mechanism 72 is connected to the rotating mechanism 73, and when in use, the rotating mechanism 73 is driven by the power mechanism 72 to rotate around its own axis, and the plurality of centrifuge tube racks 74 connected thereto are driven by the rotating mechanism 73 to rotate, so as to complete the centrifugal treatment of the centrifuge tube;

[0051] The rotating mechanism 73 is composed of a support seat 731, a rotating bracket 732 and a pressing plate 733; the support seat 731 can be rotatably installed around its own axis and is installed below the working platform, and the bottom of the support seat 731 is connected to the power mechanism 72, and the top of the support seat 731 passes through the working platform and the bottom frame 712 in turn and is placed in the centrifugal chamber; the rotating bracket 732 is fixedly installed on the top of the support seat 731, and the rotating bracket 732 is evenly provided with a number of installation bins 7321 matching the number of the centrifuge tube racks 74, and each of the installation bins 7321 can be rotatably installed with the Centrifuge tube rack 74; the pressing plate 733 is installed on the rotating bracket 732 above the centrifuge tube rack 74, and the pressing plate 733 is provided with a convex finger 7331 corresponding to the position of each installation bin 7321, and the centrifuge tube rack 74 in each installation bin 7321 is provided with a slot corresponding to the position of the convex finger 7331. When in use, the convex finger 7331 and the slot play a limiting role on the centrifuge tube rack 74, thereby realizing the control of the swing direction and angle of the centrifuge tube rack 74 during the centrifugation process, effectively avoiding collision between centrifuge tubes on two relatively arranged centrifuge tube racks 74, and ensuring the integrity of the centrifuge tubes;

[0052] The power mechanism 72 includes a servo motor 721 with an encoder, a transmission gear 722, a synchronous belt 723 and a rotating gear 724; the rotating gear 724 is fixedly mounted on the bottom of the support seat 731; the servo motor 721 is mounted on one side of the rotating gear 724; the transmission gear 722 is fixedly mounted on the output shaft of the servo motor 721; the synchronous belt 723 is arranged on the transmission gear 722 and the rotating gear 724; when in use, the servo motor 721 drives the transmission gear 722 connected thereto to rotate, and the transmission gear 722 drives the rotating gear 724 to rotate through the synchronous belt 723, thereby driving the supporting seat 731 connected thereto to rotate around its own axis through the rotating gear 724;

[0053] It is worth mentioning that the application of the encoder effectively ensures that the number of revolutions of the servo motor 721 each time is a fixed value, thereby ensuring that each centrifuge tube rack 74 returns to its initial position after completing the centrifugal treatment, thereby realizing the positioning function of the centrifuge tube rack 74;

[0054] The power mechanism 72 further includes two tightening devices 725; the two tightening devices 725 are respectively installed below the bottom frame 712 on both sides of the synchronous belt 723, and the positions of the two tightening devices 725 are adjustable, which are used to adjust the tightness of the synchronous belt 723, effectively ensuring the accuracy of the transmission;

[0055] Each centrifuge tube rack 74 is provided with a gravity pressing block 741 at the bottom. When the centrifugation work is completed, each centrifuge tube rack 74 is reset under the gravity of its own gravity pressing block 741.

[0056] The top cover 711 is provided with a cover plate receiving groove, and the cover plate receiving groove is provided with a cover plate 7111; handles 7112 are respectively provided on both sides of the cover plate 7111 for grabbing the cover plate 7111; the cover plate 7111 is also provided with an observation window 7113 between the two handles 7112, so that the operator can observe the working status in the centrifugal chamber;

[0057] Preferably, the number of the centrifuge tube racks 74 is four, and correspondingly, the number of the installation bins 7321 is also four;

[0058] The centrifuge mechanism 7 also includes a waste bucket and a waste liquid tank; the waste bucket and the waste liquid tank are arranged on the working platform from front to back between the shell 71 and the dyeing mechanism 8, the waste bucket is used to recycle the centrifuge tube, and the waste liquid tank is used to recycle the centrifuge waste liquid.

[0059] The staining mechanism 8 includes a plurality of slide staining components 81 and a bracket; the bracket is arranged on the working platform; the plurality of slide staining components 81 are arranged on the bracket for easy placement and removal;

[0060] The dyeing mechanism 8 also includes a recovery tank 82 for recovering waste liquid; the recovery tank 82 is arranged on the working platform on the right side of the bracket;

[0061] The dyeing mechanism 8 also includes a recycling bucket 83 for recycling waste materials; the recycling bucket 83 is arranged on the working platform at the rear side of the bracket;

[0062] Each of the slide staining components 81 comprises:

[0063] The dye bottle 811 is hollow, and has an annular protrusion 8111 and a rubber ring 8112 at its bottom, and a protrusion 8113 extends from opposite sides of the annular protrusion 8111;

[0064] The carrier plate 812 is provided with grooves 8121 arranged in an array, and the grooves 8121 are used to accommodate glass slides 8122. Each groove 8121 has buckle positions 8123 on both inner sides, which are used to buckle the protrusions 8113 of the annular protrusion 8111 at the bottom of the staining bottle 811;

[0065] Wherein, handles 8124 are provided on both sides of the carrier plate 812 for transferring the carrier plate 812;

[0066] Preferably, the number of the slide staining components 81 is two.

[0067] The X-axis sliding mechanism 2 includes an X-axis slide rail 21, a first motor 22 arranged at the end of the X-axis slide rail 21, and a chain 23 arranged at the output end of the first motor 22; the chain 23 is arranged on the track of the X-axis slide rail 21 and can slide back and forth along the track of the X-axis slide rail 21; the pipetting mechanism 4 is arranged on the chain 23 and can slide back and forth on the X-axis slide rail 21 along the chain 23 to move.

[0068] The Y-axis sliding mechanism 3 includes a Y-axis slide rail 31, a slider 32 slidably connected to the Y-axis slide rail 31, and a belt transmission mechanism providing power to the slider 32;

[0069] The belt transmission mechanism includes a second motor 33, a driving wheel 34 connected to the output end of the second motor 33, a driven wheel 35 matched with the driving wheel 34, and belts arranged on the driving wheel 34 and the driven wheel 35; the slider 32 is arranged on the belt 36.

[0070] The pipetting mechanism 4 includes a first pipetting component 41, a first pipetting groove 42 matched with the first pipetting component 41, a second pipetting component 43, a second pipetting groove 44 matched with the second pipetting component 43, a specimen liquid groove 45, and a connecting plate 46; the connecting plate 46 is arranged on the chain 23, and can slide forward and backward along the chain 23 on the X-axis slide rail 21 to move; the first pipetting component 41 and the second pipetting component 43 are arranged on the connecting plate 46 in sequence from front to back, and are respectively used for pipetting;

[0071] The first liquid transfer component 41 includes a third motor 411 and a first liquid transfer gun 412 connected to the output end of the third motor 411; the first liquid transfer gun 412 is also connected to the feeding mechanism 6 through a material tube; the first liquid transfer tank 42 is arranged on the working platform in front of the centrifugal mechanism 7, and the first liquid transfer tank 42 is provided with a plurality of first liquid transfer tubes matched with the first liquid transfer gun 412, and the first liquid transfer gun 412 is driven up and down by the third motor 411, so that the first liquid transfer tube can be sleeved on the head of the first liquid transfer gun 412;

[0072] The second liquid transfer assembly 43 includes a fourth motor 431 and a second liquid transfer gun 432 connected to the output end of the fourth motor 431; the second liquid transfer gun 432 is also connected to the feeding mechanism 6 through a material tube; the second liquid transfer tank 44 is arranged on the working platform at the front side of the waste bucket, and the second liquid transfer tank 44 is provided with a plurality of second liquid transfer tubes matched with the second liquid transfer gun 432, and the second liquid transfer gun 432 is driven up and down by the fourth motor 431, so that the second liquid transfer tube can be sleeved on the head of the second liquid transfer gun 432;

[0073] The specimen liquid tank 45 is disposed on the working platform between the second liquid transfer tank 44 and the staining mechanism 8 , and a plurality of specimen liquid tubes are placed in the specimen liquid tank 45 .

[0074] The injection mechanism 5 includes a mounting frame 51, a fifth motor 54 arranged on the mounting frame 51, a support plate 52 connected to the output end of the fifth motor 54, and a plurality of injection assemblies 53 matched with the grooves 8121 arranged in an array on the carrier plate 812; the mounting frame 51 is arranged on the right side of the X-axis slide rail 21, and each of the injection assemblies 53 is arranged on the support plate 52 corresponding to the position of a row of grooves 8121 on the carrier plate 812, and the support plate 52 is driven to move up and down by the fifth motor 54, and then the plurality of injection assemblies 53 arranged on the support plate 52 are driven to move up and down by the support plate 52;

[0075] Each of the injection assemblies 53 includes four groups of dyeing needles and one group of waste liquid needles, and the length of the waste liquid needles is greater than the length of the four groups of dyeing needles; each group of dyeing needles is connected to the feeding mechanism 6 through a material pipe, and then the action of injecting liquid is completed through the feeding mechanism 6; the waste liquid needles are used to suck out waste liquid.

[0076] The loading mechanism 6 includes a plurality of pressure pumps 61 that cooperate with the first pipette gun 412, the second pipette gun 432 and a plurality of injection components 53; each of the pressure pumps 61 is externally connected to a liquid storage device, the first pipette gun 412 is connected to one of the pressure pumps 61 through a material pipe, and the second pipette gun 432 is connected to one of the pressure pumps 61 through a material pipe; and the dyeing needle of each injection component 53 is respectively connected to a corresponding pressure pump 61 through a material pipe, and the operation of the dyeing needle injecting liquid is completed through the action of the pressure pump 61, and the liquid in the material pipe is recovered into the liquid storage device through the action of the pressure pump 61, thereby completing the pipe discharge operation.

[0077] The working process and principle of the present invention are as follows: first, the centrifuge tubes are manually placed on the centrifuge tube rack 74; then, after selecting the number of specimens, the staining method and the slide preparation method on the operation interface of the control device 9, the operation key is pressed to operate the device; through the action of the X-axis sliding mechanism 2 and the Y-axis sliding mechanism 3, the first liquid transfer component 41 is moved to the top of all the centrifuge tubes one by one, and the pressure pump 61 connected to the first liquid transfer gun 412 is actuated, so that the first liquid transfer gun 412 injects a fixed amount of liquid into each centrifuge tube one by one; next, through the action of the X-axis sliding mechanism 2 and the Y-axis sliding mechanism 3, the first liquid transfer component 41 is moved to the first liquid transfer tank 42 The first pipette 412 is driven downward by the third motor 411 to complete the combined action of the first pipette 412 and the first pipette. Then, the first pipette is moved to the top of the specimen liquid tube by the action of the X-axis sliding mechanism 2 and the Y-axis sliding mechanism 3. The first pipette is driven downward by the third motor 411 to make the first pipette extend into the first pipette to absorb the specimen liquid. The first pipette is driven to move to the top of the centrifuge tube by the action of the X-axis sliding mechanism 2 and the Y-axis sliding mechanism 3 to complete the action of transferring the specimen liquid to the centrifuge tube. The above actions are performed multiple times until all centrifuge tubes are injected with the specimen liquid. The liquid stops; next, the centrifugal operation is completed through the action of the centrifugal mechanism 7; after the centrifugation is completed, the upper layer of liquid in the centrifuge tube is extracted and discarded into the waste liquid tank by the first pipette; next, through the action of the X-axis sliding mechanism 2 and the Y-axis sliding mechanism 3, the second pipette component 43 is moved to the top of the second pipette tank 44, and the second pipette gun 432 is driven downward by the fourth motor 431, thereby completing the combined action of the second pipette gun 432 and the second pipette, and then, through the action of the X-axis sliding mechanism 2 and the Y-axis sliding mechanism 3, the second pipette component 43 is moved to the top of all the centrifuge tubes one by one, and is connected to the second pipette gun 432 The pressure pump 61 is actuated to make the second pipette gun 432 inject quantitative liquid into each centrifuge tube one by one. After the liquid in the centrifuge tube is mixed, the second pipette transfers the liquid quantitatively to the dyeing bottle 811 for sedimentation and slice making under the action of the X-axis sliding mechanism 2, the Y-axis sliding mechanism 3, the fourth motor 431 and the second pipette gun 432; next, the injection mechanism 5 is moved to the top of the dyeing bottle 811 through the action of the Y-axis sliding mechanism 3, and the dyeing operation according to the process is completed through the action of the feeding mechanism 6; after the slice making and dyeing are completed, the sealing operation is performed; next, the pipe discharge key is pressed on the operation interface of the control device 9 to discharge the pipe, and then the machine is turned off. Repeat the above actions to realize batch automatic slice making.

[0078] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A film making and dyeing machine, characterized in that: include: A type-A frame, an X-axis sliding mechanism, a Y-axis sliding mechanism, a pipetting mechanism, an injection mechanism, a feeding mechanism matched with the pipetting mechanism and the injection mechanism, a centrifugal mechanism, a dyeing mechanism and a control device; the Y-axis sliding mechanism is arranged on the upper part of the type-A frame, the X-axis sliding mechanism is slidably connected with the Y-axis sliding mechanism, and can slide left and right along the Y-axis direction of the Y-axis sliding mechanism; the pipetting mechanism is arranged on the left side of the X-axis sliding mechanism, and is slidably connected with the X-axis sliding mechanism, and can slide back and forth along the X-axis direction of the X-axis sliding mechanism; the injection mechanism is arranged on the right side of the X-axis sliding mechanism; the feeding mechanism is arranged on the vertical part of the type-A frame, and is connected with the pipetting mechanism and the injection mechanism; the bottom of the type-A frame is set as a working platform, and the centrifugal mechanism, the dyeing mechanism and the control device are arranged on the working platform in sequence from left to right; the control device is electrically connected with the X-axis sliding mechanism, the Y-axis sliding mechanism, the pipetting mechanism, the injection mechanism, the feeding mechanism and the centrifugal mechanism, respectively, for controlling the operation of the equipment; The X-axis sliding mechanism comprises an X-axis slide rail, a first motor arranged at the end of the X-axis slide rail, and a chain arranged at the output end of the first motor; the chain is arranged on the track of the X-axis slide rail and can slide forward and backward along the track of the X-axis slide rail; the liquid transfer mechanism is arranged on the chain and can slide forward and backward along the chain on the X-axis slide rail to move; The Y-axis sliding mechanism includes a Y-axis slide rail, a slider slidably connected to the Y-axis slide rail, and a belt transmission mechanism providing power to the slider; The belt transmission mechanism includes a second motor, a driving wheel connected to the output end of the second motor, a driven wheel matched with the driving wheel, and a belt arranged on the driving wheel and the driven wheel; the slider is arranged on the belt; The pipetting mechanism comprises a first pipetting assembly, a first pipetting trough matched with the first pipetting assembly, a second pipetting assembly, a second pipetting trough matched with the second pipetting assembly, a specimen liquid trough, and a connecting plate; the connecting plate is arranged on a chain and can slide forward and backward along the chain on an X-axis slide rail to move; the first pipetting assembly and the second pipetting assembly are arranged on the connecting plate in sequence from front to back, and are used for pipetting respectively; The first liquid transfer assembly includes a third motor and a first liquid transfer gun connected to the output end of the third motor; the first liquid transfer gun is also connected to the feeding mechanism through a material tube; the first liquid transfer tank is arranged on the working platform at the front side of the centrifugal mechanism, and the first liquid transfer tank is provided with a plurality of first liquid transfer tubes matched with the first liquid transfer gun; The second liquid transfer assembly includes a fourth motor and a second liquid transfer gun connected to the output end of the fourth motor; the second liquid transfer gun is also connected to the feeding mechanism through a material pipe; the second liquid transfer tank is arranged on the working platform, and the second liquid transfer tank is provided with a plurality of second liquid transfer tubes matched with the second liquid transfer gun; The specimen liquid tank is arranged on the working platform between the second liquid transfer tank and the staining mechanism, and a plurality of specimen liquid tubes are placed in the specimen liquid tank.

2. The film making and dyeing machine according to claim 1, characterized in that: The centrifugal mechanism comprises a shell, a power mechanism, a rotating mechanism and a plurality of centrifuge tube racks; the shell comprises a detachable top cover and a bottom frame, the top cover is placed above the bottom frame, and a centrifuge chamber is arranged in the bottom frame; the rotating mechanism is installed below the working platform, and the top of the rotating mechanism passes through the working platform and the bottom frame in turn and is placed in the centrifuge chamber; a plurality of centrifuge tube racks are rotatably installed on the top of the rotating mechanism, each of the centrifuge tube racks is evenly provided with a plurality of tube holes, and a centrifuge tube is placed in each tube hole; the power mechanism is installed below the working platform on one side of the rotating mechanism, and the power mechanism is connected to the rotating mechanism; The centrifuge mechanism also includes a waste bucket and a waste liquid tank; the waste bucket and the waste liquid tank are arranged on the working platform from front to back between the shell and the dyeing mechanism, the waste bucket is used to recycle the centrifuge tube, and the waste liquid tank is used to recycle the centrifuge waste liquid.

3. The film making and dyeing machine according to claim 2, characterized in that: The rotating mechanism consists of a supporting seat, a rotating bracket and a pressing plate; the supporting seat can be rotatably installed around its own axis and is installed below the working platform, and the bottom of the supporting seat is connected to the power mechanism, and the top of the supporting seat passes through the working platform and the bottom frame in turn and is placed in the centrifugal chamber; the rotating bracket is fixedly installed on the top of the supporting seat, and the rotating bracket is evenly provided with installation bins matching the number of the centrifuge tube racks, and the centrifuge tube rack can be rotatably installed in each of the installation bins; the pressing plate is installed on the rotating bracket above the centrifuge tube rack, and the pressing plate is provided with a convex finger corresponding to the position of each of the installation bins, and the centrifuge tube rack in each of the installation bins is provided with a slot corresponding to the position of the convex finger.

4. The film making and dyeing machine according to claim 3, characterized in that: The power mechanism includes a servo motor with an encoder, a transmission gear, a synchronous belt and a rotating gear; the rotating gear is fixedly installed at the bottom of the support seat; the servo motor is installed on one side of the rotating gear; the transmission gear is fixedly installed on the output shaft of the servo motor; the synchronous belt is arranged on the transmission gear and the rotating gear; The power mechanism further includes two tightening devices; the two tightening devices are respectively installed below the bottom frame on both sides of the synchronous belt to adjust the tightness of the synchronous belt; A gravity pressing block is provided at the bottom of each centrifuge tube rack; The top cover is provided with a cover plate receiving groove, in which a cover plate is placed; handles are respectively provided on both sides of the cover plate for grabbing the cover plate; and an observation window is also provided on the cover plate between the two handles.

5. The film making and dyeing machine according to claim 1, characterized in that: The staining mechanism comprises a plurality of glass slide staining components and a bracket; the bracket is arranged on a working platform; and a plurality of glass slide staining components are arranged on the bracket; The dyeing mechanism also includes a recovery tank for recovering waste liquid; the recovery tank is arranged on the working platform on the right side of the bracket; The dyeing mechanism also includes a recycling bucket for recycling waste materials; the recycling bucket is arranged on the working platform at the rear side of the bracket; Each of the slide staining components comprises: The dyeing bottle is hollow, with an annular protrusion and a rubber ring at the bottom, and a protrusion extending from each of the two opposite sides of the annular protrusion; A carrier plate is provided with grooves arranged in an array, the grooves are used to accommodate glass slides, and each groove has buckle positions on both inner sides, which are used to buckle the annular protrusions on the bottom of the staining bottle; Wherein, handles are provided on both sides of the carrier plate for transferring the carrier plate.

6. The film making and dyeing machine according to claim 5, characterized in that: The injection mechanism includes a mounting frame, a fifth motor arranged on the mounting frame, a support plate connected to the output end of the fifth motor, and a plurality of injection assemblies matched with the grooves arranged in an array on the carrier; the mounting frame is arranged on the right side of the X-axis slide rail, and each of the injection assemblies is arranged on the support plate corresponding to the position of a row of grooves on the carrier; Each of the injection assemblies comprises a plurality of groups of dyeing needles and a group of waste liquid needles, and the length of the waste liquid needles is greater than the length of the plurality of groups of dyeing needles; each group of dyeing needles is connected to the feeding mechanism through a material pipe; and the waste liquid needles are used to suck out waste liquid.

7. The sheet making and dyeing machine according to claim 6, characterized in that: The loading mechanism includes several pressure pumps that cooperate with the first pipette, the second pipette and several injection components; each of the pressure pumps is externally connected to a liquid storage device, the first pipette is connected to one of the pressure pumps through a material pipe, the second pipette is connected to one of the pressure pumps through a material pipe, and the dyeing needle of each injection component is connected to a corresponding one of the pressure pumps through a material pipe.

Citation Information

Patent Citations

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