Culture medium filtration equipment

Abstract

The invention relates to a culture medium filtration device (1) and a method for culture medium filtration, which utilizes a prefabricated preparation container (2) having an inflow (3) for a solvent (30) and an outflow (4) for a solution (31). A preparation chamber (5) containing a culture medium (29) to be dissolved is located between the inflow (3) and the outflow (4). The preparation chamber (2) has a filter (22). At least one mixing arm (6, 7) connected to the inflow (3) and at least one mixing nozzle (19, 20) extend into the culture medium (29) to be dissolved in the preparation chamber (5), wherein the at least one mixing nozzle (19, 20) is arranged at the free end (17, 18) of the mixing arm and is configured to generate a directional solvent jet. The preparation container (2) has a bottom (10) and a top (8), wherein the preparation chamber (5) is located between the bottom (10) and the top (8). The invention is characterized in that the filter (22) is in the form of a sterile filter; the filter (22) has a circumferential outer edge (23) on the filtrate side facing the outflow portion (4), by which the filter rests on the circumferential rest edge (37) of the bottom (10) of the preparation container (2) and is fixedly connected to the circumferential rest edge (37); and a sterilized filtrate space (39) is formed in the bottom (10) between the filtrate side (38) of the filter (22) and the outflow portion (4).

Description

Technical Field

[0001] The present invention relates to a culture medium filtration device, which has an assembled preparation container, the preparation container has an inlet for a solvent and an outlet for a solution, wherein a preparation chamber with a culture medium to be dissolved is arranged between the inlet and the outlet, wherein the preparation container has a filter, and wherein at least one mixing arm connected to the inlet protrudes with at least one mixing nozzle into the culture medium to be dissolved in the preparation chamber, wherein the at least one mixing nozzle is arranged at the free end of the at least one mixing arm and is used to generate a directional solvent jet, wherein the preparation container has a base and a cover, and the preparation chamber is arranged between the base and the cover.

[0002] The invention also relates to a method for filtering culture medium using the culture medium filtering device. Background Art

[0003] In the pharmaceutical and biopharmaceutical fields, especially in the preclinical or clinical field, as well as in the field of research and development, it is often necessary to prepare small amounts of buffers and culture media. Buffers and culture media are often already prepared according to a predetermined recipe, which requires careful and relatively time-consuming work by professionals. Particularly important here is the preparation of substance mixtures that match the volume, concentration and composition or culture media required by the end consumer.

[0004] If liquid (that is to say solvent) is fed only when needed, the volume of the storage container can be relatively small, and therefore can be transported and stored more easily. In addition, because usually owing to dry storage, cooling is not required, storage and transportation are further facilitated.

[0005] EP 2 236 437 B1 discloses a culture medium filtration device having an already assembled preparation container with an inflow for a solvent and an outflow for a solution. In this case, a preparation chamber with a culture medium to be dissolved is arranged between the inflow and the outflow, and the preparation container has a filter. A mixing arm connected to the inflow protrudes into the preparation chamber with a mixing nozzle arranged at the free end of the mixing arm.

[0006] A disadvantage of this known culture medium filtration device is that the antechamber, which is subdivided by a filter (which may be an antibacterial filter), is arranged between the inflow and the preparation chamber. Firstly, this is relatively complex and therefore cost-intensive, and secondly, the filter is arranged adjacent to the inflow and not adjacent to the outflow. This is disadvantageous in particular in the pharmaceutical and biopharmaceutical fields, since the sterility of the filtrate chamber arranged upstream of the outflow is not provided.

[0007] In addition, EP 2 244 743 B1 has disclosed a culture medium filtering device, which has an assembled preparation container, which has an inflow side and an outflow for a solution, and the inflow for a solvent can be realized into the inflow side via an insertable sleeve. Here too, a preparation chamber with a culture medium to be dissolved is arranged between the inflow and the outflow. Therefore, the solvent is fed via a sleeve inserted into the preparation chamber. The preparation chamber has a filter toward the outflow, which is preferably spaced apart from the outlet side.

[0008] The disadvantage here is that such a capsule configuration for food cannot be used for the use of cell culture media. For cell culture media, it is particularly important that the culture media is provided in a sterile state. However, sterilization of the cell culture media is often not possible because the cell culture media is destroyed or at least damaged during the sterilization process, that is, the unit cannot be sterilized as a whole.

[0009] A capsule for producing beverages is known from WO 2017 / 050 745 A1. The beverage capsule comprises a cup-shaped body which forms a chamber and is closed by a cover through which a liquid can be injected into the chamber of the capsule. A partition wall subdivides the interior of the beverage capsule into a relatively small antechamber arranged below the cover and a relatively large main chamber arranged below the antechamber, in which individual portion quantities of the beverage components are located. Located in the partition wall is a channel which projects into the main chamber and in this way connects the two chambers to each other and allows the throughflow of the injected liquid from the antechamber into the main chamber.

[0010] US 3 592 245 A has disclosed a pharmaceutical container for producing a pharmaceutical solution from at least two components in the container under aseptic conditions. One component of the pharmaceutical solution has been placed in a bottle-shaped container. The container is provided with a cover having a chamber inwardly and adjacent to a hollow piercing device, which selectively extends into the container or faces in another direction, that is, away from the container. A filter is arranged between the chamber of the cover and the chamber of the piercing device.

[0011] US 4 475 914 A has disclosed a pharmaceutical container for producing a pharmaceutical solution from a solid component of relatively low solubility and a liquid, which is connected to a hollow piercing device. The piercing device comprises two channels which can optionally be ventilated via valves provided with sterile filters.

[0012] US 2003 / 0 029 763 A1 has disclosed a packaging for a sterile disposable syringe filter, which allows the connection of the syringe filter to a syringe without having to put down the syringe for the purpose of opening the filter packaging. In this case, the filter packaging has a penetration point at which the connection for the filter of the syringe is located. In addition, the filter packaging is provided with a preformed tear line along which the packaging can be torn, which allows simple removal of the syringe filter connected to the syringe.

[0013] DE 10 2011 112 291 A1 has disclosed a device for filtering a liquid sample, which has a pressurizable sample container for receiving the liquid sample and has a filter attachment part comprising a filter, which can be connected to a connection part of the sample container. The sample container contains a filter aid suitable for pre-coat filtration. In addition, the filter attachment part has a dead zone, which is arranged upstream of the filter of the filter attachment part and is used to receive the filter cake being formed. Summary of the invention

[0014] Purpose of the Invention

[0015] The object of the present invention is to improve the known culture medium filtration device so that, for an inexpensive design of the culture medium filtration device, the sterility of the filtrate in the filtrate space can be ensured even if the culture medium to be dissolved (e.g. cell culture medium) cannot be sterilized, that is, will be destroyed or at least damaged during the sterilization process.

[0016] Another object of the present invention is to specify a method for culture medium filtration using such an improved culture medium filtration device, in which the sterility of the filtrate in the filtrate space can be ensured even if the culture medium to be dissolved (e.g. cell culture medium) cannot be sterilized, that is, will be destroyed or at least damaged during the sterilization process.

[0017] Invention Introduction

[0018] In combination with the features of the present invention, the purpose of a culture medium filtration device is achieved, wherein the filter is in the form of a sterile filter, wherein the filter has a surrounding outer boundary on its filtrate side facing the outflow portion, through which the filter is supported on the surrounding support boundary of the base and fixedly connected to the surrounding support boundary, and wherein a sterilized filtrate space is formed between the filtrate side of the filter and the outflow portion, and the filtrate space is arranged in the base.

[0019] A mixing arm connected to the inflow and a mixing nozzle arranged at the free end of the mixing arm make it possible to mix the culture medium to be dissolved with the solvent in the preparation chamber. A mixing arm is to be understood as meaning a fixed feed with a feed channel connected thereto, the free end of which ends in the preparation chamber and has a mixing nozzle via which a directed solvent jet can be fed into the preparation chamber.

[0020] The design of the filter as a sterile filter ensures that the filtered solution is sterile. The surrounding outer boundary on the filtrate side of the filter makes it possible to sterilize the base with the filter and the filtrate space separately, wherein the filter is fixedly connected to the surrounding support boundary of the base by this outer boundary. It is clear to those skilled in the art that in order to ensure the sterility of the filtrate space, it must be sealed relative to the outside, that is to say not open, as shown in the accompanying drawings. This can be achieved, for example, via a cover, a sterile connector or a seal (peel-off film / film) on the connection.

[0021] Therefore, it is possible to dispense with sterilizing the culture medium and still ensure the sterility of the solution (that is to say the filtrate). The culture medium to be dissolved is arranged in the preparation chamber on the non-filtrate side of the filter. The design of the filter as a sterile filter allows the sterility of the solution of the dissolved filtered culture medium to be ensured in a relatively simple and inexpensive manner. With the culture medium filtration device according to the present invention, the high requirements of bioprocessing technology can be met, particularly in terms of sterility and safety to prevent leakage of undesirable substances, which is particularly important in this field.

[0022] According to a preferred embodiment of the present invention, agitation particles that cannot be dissolved by the solvent are arranged in the preparation chamber.

[0023] By means of the solvent-insoluble stirring particles arranged in the preparation chamber, the solvent leaving the mixing nozzle is swirled together with the culture medium to be dissolved, and clogging of the filter is reliably prevented. While the culture medium is dissolved in the fed solvent and discharged as a filtered solution via the outflow section, the stirring particles remain in the preparation chamber.

[0024] According to another preferred embodiment of the present invention, the mixing nozzle is arranged in the preparation chamber so that it produces a directional solvent jet, which moves the culture medium to be dissolved on the filter in the form of a circulating flow. Here, turbulence leads to rapid and thorough mixing and shortens the dissolution time.

[0025] According to a further preferred embodiment of the invention, the base has a channel system which leads to the outflow and is open towards the filtrate side of the filter.

[0026] By means of the channel system which is open towards the filtrate side of the filter and opens into the outflow, the filtrate is discharged relatively uniformly over the entire filter surface and can therefore be used accordingly.

[0027] According to a preferred embodiment of the present invention, the preparation container has a ventilation device, at least one ventilation opening of which is covered by a hydrophobic filter.

[0028] In order to produce an exact concentration of the solution to be produced, it is particularly important not only to maintain an exact amount of dry culture medium available, but also to maintain an exact volumetric dosing. The aerator leads to a technically simplified solution which avoids a dosing device or a valve device arranged downstream of the culture medium filtration unit and by which the formation of a compressed air cushion by adding solvent to the culture medium filtration device, which would lead to a subsequent flow and thus to an incorrect liquid dosing after the feed of the solvent has stopped, can be reliably excluded. In addition, the hydrophobic filter reliably prevents the solvent or the reconstitution culture medium from being able to escape via the aerator.

[0029] According to a preferred embodiment of the present invention, the inflow portion is arranged on the cover of the preparation container. Moreover, the cover may have the above-mentioned ventilation device, through which the air displaced by the solvent can escape. Here, the ventilation device has at least one ventilation opening, which is covered by a hydrophobic filter on the inner surface of the cover facing the preparation chamber.

[0030] According to a preferred embodiment, the cover has an open channel system via which the air to be exhausted from the preparation chamber is guided to the hydrophobic filter.

[0031] According to another preferred embodiment of the present invention, the filter in the form of a sterile filter has a multi-layer form. Preferably, at least one layer of the filter is a porous membrane. Alternatively, a multi-layer nonwoven can also be used. Other layers with filtering properties known to those skilled in the art are also conceivable.

[0032] The sterile filter preferably has an average pore size in the range of 0.1 μm to 8 μm. The average pore size is determined, for example, by the following method for determining the average pore size.

[0033] Capillary flow porosimetry was used. Capillary flow porosimetry is a gas / liquid porosimetry method in which the differential pressure and flow rate of a gas through a membrane sample are measured first in a wet state and then in a dry state.

[0034] Prior to the measurement, the membrane sample is brought into contact with the wetting liquid so that all available pores are filled with the liquid. After the pores have been filled and the sample has been introduced, the measuring cell will be closed and the measurement will begin. After the measurement begins, the air pressure will automatically and gradually increase and the air pressure will empty the pores with a diameter corresponding to the prevailing pressure. This is done until the relevant pore range has been covered, that is, until even the smallest pores present in the measurement range are free of liquid. Subsequently, the pressure is reduced again and the measurement is automatically repeated on the now dry sample. The pore size distribution is calculated from the difference between the two pressure-flow curves via the Young-Laplace equation (see Shrestha, Aabhash, Mechanical Engineering Graduate Theses & Dissertations, Paper 38, "Characterization of porous membranes via porometry" (2012)).

[0035] According to a preferred embodiment of the invention, two mixing arms are arranged in the preparation chamber, which are connected to the inflow and have in each case at least one mixing nozzle arranged at their free ends. Here, one or more mixing nozzles are advantageously arranged eccentrically with respect to the longitudinal axis of the preparation container. The eccentric arrangement of the mixing nozzles leads to better vortexing and therefore to faster mixing of the solvent with the culture medium to be dissolved and therefore to faster dissolution of the culture medium. However, it is also basically possible to arrange three or more mixing arms connected to the inflow in the preparation chamber and have in each case at least one mixing nozzle arranged at their free ends.

[0036] According to another preferred embodiment of the invention, the inflow extends centrally relative to the longitudinal axis of the preparation container into the preparation chamber and transitions to, for example, two mixing arms, which are each spread apart with their mixing arm axes at a spread angle between 15° and 90°, for example 60°, relative to the longitudinal axis. Preferably, the two mixing arms span a vertical plane, the mixing nozzles being oriented orthogonally relative to the vertical plane and in mutually opposite spray directions. This ensures a turbulent flow of the solvent with the culture medium to be dissolved and the agitated particles, as well as a brief shortening of the mixing process.

[0037] The culture medium to be dissolved may be a powder or a granule. Furthermore, the culture medium to be dissolved may contain additives.

[0038] In combination with the features of the present invention, the purpose of a culture medium filtration method using such a culture medium filtration device is achieved, wherein the inflow part of the culture medium filtration device is connected to a storage container, the solvent is fed from the storage container to the preparation container via a regulating valve controlled by a control unit, and wherein the outflow part of the culture medium filtration device is connected to a receiving container, and the filtered solution is fed to the receiving container.

[0039] In a preferred refinement of the method, the following steps are carried out for the pre-assembly preparation of the container:

[0040] a) providing a cover and a base with a sterilized filtrate space,

[0041] b) introducing the culture medium to be lysed into the preparation chamber, and

[0042] c) Joining the cover and the base together.

[0043] The base with outflow, filter and filtrate space forms a sterilizable unit, which can be sterilized without a cover and without culture medium to be dissolved, which means that a sterilized (that is to say aseptic) filtrate space can be formed without any problem. The base can be packaged separately or inserted with the cover for transportation for pre-assembly. The culture medium to be dissolved is introduced into a preparation chamber opened on one side, and then, the cover and the base are combined together. Combining together can be achieved, for example, by adhesive bonding or by laser welding or by ultrasonic welding.

[0044] In step b), stirring particles which are insoluble in the solvent can additionally be introduced.

[0045] The already assembled culture medium filtration device can be packaged, stored and transported without any problems.

[0046] Additional features and advantages of the present invention will become apparent from the following detailed description and the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In the attached picture:

[0048] Figure 1 shows a cross-sectional side view of a media filtration apparatus;

[0049] Figure 2 Shows a filter and a sterilized filtrate space Figure 1 The base of the middle;

[0050] Figure 3 Shows Figure 1 The base with the sterilized filtrate space and the installed cover, still free of culture medium and particles;

[0051] Figure 4 shows a cross-sectional side view of another media filtration device;

[0052] Figure 5 Shows a filter and a sterilized filtrate space Figure 4 The base of the middle;

[0053] Figure 6 A schematic diagram of a media filtration apparatus is shown, the apparatus having an upstream controlled solvent reservoir and a downstream collection vessel for the filtered solution, and

[0054] Figure 6a Shown according to Figure 6 Another schematic diagram of a culture medium filtration device. DETAILED DESCRIPTION

[0055] The culture medium filtration device 1 basically comprises a preparation container 2 , an inflow part 3 , an outflow part 4 , a preparation chamber 5 and two mixing arms 6 , 7 .

[0056] The preparation container 2 includes a cover 8 having an inlet 3. Figures 1 to 4 In an exemplary embodiment of the invention, the inflow portion is in the form of a pipe connection portion. Figures 1 to 3 , an annular container wall 9 is integrally formed on the cover 8. The container wall 9 is connected at its lower end in the vertical direction to a base 10, which has an outflow portion 4. The cover 8, the container wall 9 and the base 10 define a preparation chamber 5.

[0057] according to Figure 4 and Figure 5 , an annular container wall 9' is integrally formed on the base 10'. The container wall 9' is connected to the cover 8' having the inflow portion 3 at its upper end in the vertical direction. The cover 8', the container wall 9' and the base 10' define the preparation chamber 5.

[0058] The inflow 3 projects centrally into the preparation chamber 5 via a carrier 12 relative to the longitudinal axis 11 of the preparation container 2. At the end of the carrier 12, the carrier 12 transitions into the mixing arms 6, 7. The first mixing arm has a first mixing arm axis 13, and the second mixing arm 7 has a second mixing arm axis 14. The mixing arm axes 13, 14 each diverge at a spread angle 15, 16 relative to the longitudinal axis 11, which at the same time forms the longitudinal axis of the central carrier 12. The spread angles 15, 16 should expediently be between 15° and 90°. In the exemplary embodiment, the spread angles 15, 16 are 60° in each case for both mixing arm axes 13, 14. The mixing arms 6, 7 each have a transverse mixing nozzle 19, 20 at their free ends 17, 18. Here, the mixing arms 6, 7 span a vertical plane, the mixing nozzles 19, 20 being oriented orthogonally relative to the vertical plane and in opposite spray directions.

[0059] The base 10, 10' has a channel system 21 towards the preparation chamber 5, which leads to the outflow 4 and is covered by a filter 22. The filter 22 in the form of a sterile filter is carried by the base 10, 10' with the channel system 21, wherein the filter 22 has a surrounding outer boundary 23 on its filtrate side 38 facing the outflow 4, by means of which the filter is supported on a surrounding support boundary 37 of the base 10, 10' of the preparation container 2 and is fixedly connected to this support boundary 37.

[0060] By sterilizing the base 10, 10' with the filter 22, a sterilized filtrate space 39 is formed between the filtrate side 38 of the filter 22 and the outflow 4 arranged in the base 10, 10'. Thus, the filtrate leaving the filter 22 is fed to the outflow 4 as the solution 31 via the channel system 21, while ensuring the sterility of the solution 31.

[0061] The cover 8, 8' has a ventilation device 24, the ventilation opening 25 of which is covered by a hydrophobic filter 27, which is annular in the exemplary embodiment and is located on the inner surface 26 of the cover 8, 8' facing the preparation chamber 5. The cover 8, 8' has an open channel system 28 concentric with the hydrophobic filter 27, via which the air to be exhausted from the preparation chamber 5 is guided to the hydrophobic filter 27.

[0062] In the preparation chamber 5, a culture medium 29 is arranged, which is dissolved by a solvent 30 fed via the inflow portion 3 and discharged as a solution 31 via the outflow portion 4. Agitation particles 32 that cannot be dissolved by the solvent 30 are arranged in the preparation chamber 5.

[0063] For the purpose of medium filtration, the inflow 3 of the medium filtration device 1 is connected to a storage container 33, from which the solvent 30 is fed to the preparation container 2 via a regulating valve 34 controlled by a control unit 35. The outflow 4 of the medium filtration device 1 is connected to a receiving container 36, into which the filtered solution 31 is fed.

[0064] Storage container 33 is also to be understood as meaning a fluid preparation system and, if the fluid (solvent 30) is water, also a water preparation system for analytical pure water, such as that manufactured by Sartorius AG in Göttingen under the trade name Water preparation systems sold under the name of.

[0065] In order to pre-assemble the culture medium filtration device 1, the cover 8, 8' and the base 10, 10' with the fixedly arranged filter 22 can be loosely inserted together (e.g., sterile packaging) for delivery. That is, at least the base 10, 10' with the filter 22 and the filtrate space 39 must be sterile or sterilized.

[0066] The cover 8 , 8 ′ (if in the inserted state) is lifted off the cover 10 , 10 ′ with the sterilized filtrate space 39 , and the culture medium 29 to be dissolved and possibly the stirring particles 32 are introduced into the preparation chamber 5 .

[0067] Subsequently, the cover 8, 8' is remounted on the base 10, 10' and bonded together. The cover 8, 8' and the base 10, 10' may be bonded together, for example, by laser welding, ultrasonic welding or by adhesive bonding.

[0068] It goes without saying that the embodiments discussed in the detailed description and shown in the accompanying drawings represent only illustrative exemplary embodiments of the present invention. According to the content disclosed herein, a variety of possible variations are provided to those skilled in the art. The features of the preferred embodiments can of course also be combined with each other.

[0069] In particular, in another preferred embodiment of the invention, the preparation container 2 and / or the receiving container 36 can have at least one sensor 40, 41 for determining parameters of the prepared solution 31. Here, pH, temperature, conductivity or other parameters can be measured and possibly regulated via the control and monitoring unit 35. For example, a biochip 42, 43 for determining sterility can also be arranged in the preparation container 2 and / or the receiving container 36.

[0070] The sensor 40 is capable of monitoring the complete transfer of the culture medium 29 to be dissolved via the sterile filter 22. Here, the sensor 40 can be arranged in the non-sterile preparation chamber 5 and communicate with the control unit 35. In conjunction with the control unit 35, the sensor 40 can track the conductivity in the preparation chamber 2 and confirm the complete transfer of the culture medium 29 by falling below a threshold value.

[0071] Reference numerals list

[0072] 1 Culture medium filtration device

[0073] 2. Prepare the container

[0074] 3 Inflow

[0075] 4 outflow part

[0076] 5 Preparation Room

[0077] 6 (First) Mixing Arm

[0078] 7 (Second) Mixing Arm

[0079] 8,8'2 cover

[0080] 9,9' container wall

[0081] 10,10'2 base

[0082] 11 2 longitudinal axis

[0083] 12 3 bearing parts

[0084] 13 6 Mixing arm axis

[0085] 14 7 mixing arm axis

[0086] 15 13 expansion angle

[0087] 16 14 expansion angle

[0088] 17 6 free end

[0089] 18 7 free end

[0090] 19 6 Mixing nozzle

[0091] 20 7 Mixing nozzle

[0092] 21 10 Channel System

[0093] 22 Filters

[0094] 23 22 outer boundary

[0095] 24 8 ventilation device

[0096] 25 24 ventilation openings

[0097] 26 8 inner surface

[0098] 27 Hydrophobic filter

[0099] 28 8 channel system

[0100] 29 Culture medium

[0101] 30 Solvent

[0102] 31 Solution

[0103] 32 Stirring particles

[0104] 33 Storage Container

[0105] 34 Control valve

[0106] 35 Control Unit

[0107] 36 Receiving container

[0108] 37 10, 10' support boundary

[0109] 38 22 filtrate side

[0110] 39 Filtrate space

[0111] 40 Sensors

[0112] 41 Sensors

[0113] 42 Biochip

[0114] 43 Biochip

Claims

1. A culture medium filtration device (1), comprising an assembled preparation container (2), the preparation container (2) having an inflow portion (3) for a solvent (30) and an outflow portion (4) for a solution (31), wherein: A preparation chamber (5) with a culture medium (29) to be dissolved is arranged between the inflow (3) and the outflow (4), wherein the preparation container (2) has a filter (22), and wherein at least one mixing arm (6, 7) connected to the inflow (3) projects into the culture medium (29) to be dissolved in the preparation chamber (5) with at least one mixing nozzle (19, 20), the at least one mixing nozzle (19, 20) being arranged at the free end (17, 18) of the at least one mixing arm and serving to generate a directed jet of solvent, wherein the preparation container (2) has a base (10) and a cover (8), the preparation chamber (5) being arranged between the base (10) and the cover (8), It is characterized in that The filter (22) is in the form of a sterile filter, The filter (22) has, on its filtrate side facing the outflow (4), a surrounding outer border (23), by means of which the filter is supported on a surrounding support border (37) of the base (10) and is fixedly connected to the surrounding support border (37), and A sterile filtrate space (39) is formed between the filtrate side (38) of the filter (22) and the outflow (4), and the sterile filtrate space (39) is arranged in the base (10).

2. The culture medium filtration device (1) according to claim 1, It is characterized in that Agitation particles (32) that cannot be dissolved by the solvent (30) are arranged in the preparation chamber (5).

3. The culture medium filtration device (1) according to claim 1 or 2, It is characterized in that The mixing nozzles (19, 20) are arranged in the preparation chamber (5) such that they generate a directed solvent jet which moves the culture medium (29) to be dissolved over the filter (22) in the form of a circulating flow.

4. The culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that The base (10) has a channel system (21) which leads to the outflow (4) and opens toward the filtrate side of the filter (22).

5. The culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that The preparation container (2) has a ventilation device (24), at least one ventilation opening (25) of the ventilation device (24) is covered by a hydrophobic filter (27).

6. The culture medium filtration device (1) according to claim 5, It is characterized in that The inflow portion (3) is arranged on the cover portion (8) of the preparation container (2), and The cover (8) has the ventilation device (24), and at least one ventilation opening (25) of the ventilation device (24) is covered by the hydrophobic filter (27) on the inner surface of the cover (8) facing the preparation chamber (5).

7. The culture medium filtration device (1) according to claim 6, It is characterized in that The cover (8) has an open channel system (28) via which the air to be exhausted from the preparation chamber (5) is guided to the hydrophobic filter (27).

8. Culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that The filter (22) has a multi-layer form.

9. The culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that The filter (22) comprises one or more layers of filtering nonwoven fabric.

10. Culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that Two mixing arms (6, 7) are arranged in the preparation chamber (5), which are connected to the inflow (3) and have in each case at least one mixing nozzle (19, 20) which is arranged at the free ends (17, 18) of the two mixing arms (6, 7).

11. Culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that The mixing nozzle is arranged eccentrically with respect to the longitudinal axis (11) of the preparation container (2).

12. The culture medium filtration device (1) according to claim 11, It is characterized in that The inflow (3) projects centrally into the preparation chamber (5) relative to the longitudinal axis (11) of the preparation container (2) and transitions into the mixing arms (6, 7), the mixing arms (6, 7) and their mixing arm axes (13, 14) each diverging at a spreading angle (15, 16) between 15° and 90° relative to the longitudinal axis (11).

13. The culture medium filtration device (1) according to claim 12, It is characterized in that The mixing arms (6, 7) span a vertical plane, and the mixing nozzles (19, 20) are oriented orthogonally relative to the vertical plane and are in opposite spraying directions.

14. Culture medium filtration device (1) according to one of claims 1 to 2, It is characterized in that The culture medium (29) to be dissolved is powder or granules.

15. A method for culture medium filtration using a culture medium filtration device (1) according to one of claims 1 to 14, It is characterized in that The inflow part (3) of the culture medium filtration device (1) is connected to a storage container (33), the solvent (30) is fed from the storage container (33) to the preparation container (2) via a regulating valve (34) controlled by a control unit (35), and the outflow part (4) of the culture medium filtration device (1) is connected to a receiving container (36), and the filtered solution (31) is fed to the receiving container (36).

16. The method according to claim 15, It is characterized in that In order to pre-assemble the preparation container (2), the following steps are performed: a) providing the cover part (8) and the base part (10) having the sterilized filtrate space (39), b) introducing the culture medium to be dissolved (29) into the preparation chamber (5), and c) combining the cover (8) and the base (10) together.

17. The method according to claim 16, It is characterized in that In step b), stirring particles (32) which are insoluble in the solvent (30) are additionally introduced.

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