Nucleic acid amplification detection device

Abstract

The present invention provides a nucleic acid amplification detection device, including a sealed shell and a nucleic acid amplification reaction tube. After the nucleic acid amplification reaction tube is inserted into the plug-in channel, it can be punctured by a puncturing mechanism, so that the reaction liquid flows into the inner cavity. A liquid storage tank and a test paper are provided in the inner cavity. The upper surface of the liquid storage tank is sealed by a closed membrane. The test paper is arranged on the closed membrane and can react with the reaction liquid. A pressing portion with a pressing rod is provided in the inner cavity. The pressing rod includes a pressing end and a cone end. After the reaction is completed, the button at the pressing end causes the cone end of the cone end to tilt up and puncture the closed membrane. With the above technical solution, when the test paper completes the detection, pressing the button drives the cone end of the cone end to tilt up and puncture the closed membrane, so that the liquid in the liquid storage tank reacts with the reaction liquid on the test paper and in the nucleic acid amplification reaction tube, completely removing the residual nucleic acid in the nucleic acid amplification detection device, and avoiding the nucleic acid amplification reaction tube from accidentally falling off, or the device is damaged, so that the interior is exposed and any contamination is caused.

Description

Technical Field

[0001] The present invention relates to the field of detection, and in particular to a nucleic acid amplification detection device. Background Art

[0002] Nucleic acid diagnosis is one of the most dynamic sub-sectors in the IVD (in vitro diagnostic) industry in the future. The increase in the prevention and control of infectious diseases in my country, the promotion of blood screening nucleic acid testing, and the development of personalized medicine are the main driving forces for the development of nucleic acid diagnosis in China. Driven by these factors, the future growth rate of domestic nucleic acid diagnosis will be between 25-30%, significantly exceeding the average growth rate of the domestic IVD industry. On the one hand, nucleic acid diagnosis benefits large medical centers and realizes early, rapid, specific, and high-throughput detection of pathogens, genetic diseases, etc.

[0003] POCT (point-of-care testing), which means "immediate testing" in Chinese, is an emerging sub-industry of in vitro diagnostics (IVD). It is a new method that analyzes samples immediately at the sampling site, eliminating the complicated processing procedures of specimens in laboratory tests and quickly obtaining test results. The main standard of POCT is that it does not require a fixed testing site, and the reagents and instruments are portable and can be operated in a timely manner. POCT assumes the functions of a laboratory but does not require traditional hospital laboratory equipment. It can serve patients 24 hours a day without time or location restrictions.

[0004] However, these nucleic acid amplification methods have the problem of cross-contamination of amplified products, and false positive signals generated by product contamination can cause misinterpretation of test results. Cross-contamination between samples is often seen during the target nucleic acid amplification operation. The contamination may come from known or unknown positive substances introduced during the processing of negative samples, which cause false positive reactions through air contamination or aerosols.

[0005] A series of methods have been developed in the prior art to prevent cross-contamination of amplification products. For example, Reference 1 (CN105199940A) discloses a contamination-proof portable gene detection method and device, through which a nucleic acid amplification tube containing amplification products can be placed in the device and sealed, and then the nucleic acid amplification tube can be punctured to achieve detection. Prevent contamination of nucleic acid amplification products and avoid false positives. However, in this device, since the nucleic acid amplification tube is placed in the device and sealed, the puncture operation is difficult, and the amplification product after the test is completed still remains in the device. Once damaged, the amplification product may also diffuse into the air, causing a false positive reaction.

[0006] In addition, reference 2 (CN203241416U) also discloses a closed plastic card box for testing paper, and reference 3 (CN205574438U) also discloses a sealed test tube assembly containing a tube breaking mechanism. These detection devices are relatively convenient to operate, but the amplification product also remains in the device after the test, which poses a risk of contamination.

[0007] Therefore, providing a nucleic acid detection device with a lower possibility of contamination before and after detection has become an urgent problem to be solved in the field. Summary of the invention

[0008] The purpose of the present invention is to solve the problem that the closed detection device in the prior art is inconvenient to operate and there is still the possibility of contamination after detection. In order to solve the above problems, the present invention discloses a nucleic acid amplification detection device, which is easy to operate and can effectively prevent the amplification product from diffusing into the air and causing a false positive reaction.

[0009] In order to solve the above problems, the present invention discloses a nucleic acid amplification detection device, comprising a sealed shell and a nucleic acid amplification reaction tube, wherein an inner cavity is provided in the sealed shell, a plug-in portion is provided on the sealed shell, a plug-in channel is provided in the plug-in portion, and a puncturing mechanism is provided at the lower end of the plug-in channel. After the nucleic acid amplification reaction tube is inserted into the plug-in channel, it is sealed and connected with the plug-in channel and can be punctured by the puncturing mechanism, so that the reaction liquid flows into the inner cavity from the liquid outlet of the puncturing mechanism.

[0010] A liquid storage tank and a test paper are provided in the inner cavity. The upper surface of the liquid storage tank is sealed by a sealing membrane. The test paper is arranged on the sealing membrane and can react with the reaction liquid.

[0011] A pressing part is provided in the inner cavity, and the pressing part is located at one end of the inner cavity close to the plug-in part. The pressing part includes a pressing rod, and the pressing rod includes a pressing end and a cone end. The middle part of the pressing rod is rotatably sealed and connected to the side wall of the liquid storage tank through a pivot. The pressing end is located in the inner cavity, and the cone end is located in the liquid storage tank. The cone end is provided with a cone, and the cone can pierce the closed film. The pressing end is provided with a button.

[0012] After the reaction is completed, press the button to tilt the cone at the end of the cone and pierce the sealing membrane.

[0013] With the above technical solution, when the test strip has completed the test, pressing the button causes the cone at the end of the cone to tilt up, puncturing the sealing membrane, so that the liquid in the liquid reservoir reacts with the reaction liquid on the test strip and in the nucleic acid amplification reaction tube, completely removing the residual nucleic acid in the nucleic acid amplification detection device. In this way, even if the nucleic acid amplification reaction tube accidentally falls off or the nucleic acid amplification detection device is damaged in the subsequent process, causing the internal part to be exposed, no contamination will be caused.

[0014] According to another specific embodiment of the present invention, the pressing end is provided with an elastic support member, and the elastic support member is located below the button.

[0015] According to another specific embodiment of the present invention, a flip cover is provided at one end of the sealed housing, and the flip cover is located above the button to prevent the button from being misoperated.

[0016] According to another specific embodiment of the present invention, an elastic pressing member is disposed at the lower end of the plug-in portion, and the elastic pressing member is used to press down the sealing film and the test paper.

[0017] According to another specific embodiment of the present invention, a sealing ring made of an elastomer is provided inside the insertion channel, or a sealing ring made of an elastomer is provided outside the side wall of the nucleic acid amplification reaction tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments:

[0019] Figure 1 It is a schematic diagram of the three-dimensional structure of the nucleic acid amplification detection device provided by the present invention;

[0020] Figure 2 is a cross-sectional view of the nucleic acid amplification detection device provided by the present invention;

[0021] Figure 3 is a cross-sectional view of a state in which a sealing membrane in the nucleic acid amplification detection device provided by the present invention is punctured;

[0022] Figure 4 is a cross-sectional view of a nucleic acid amplification reaction tube provided by the present invention;

[0023] Figure 5 It is a structural schematic diagram of the nucleic acid amplification reaction tube provided by the present invention. DETAILED DESCRIPTION

[0024] The following specific embodiments illustrate the implementation of the present invention, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Although the description of the present invention will be introduced in conjunction with the preferred embodiment, this does not mean that the features of this invention are limited to this implementation. On the contrary, the purpose of introducing the invention in conjunction with the implementation is to cover other options or modifications that may extend based on the claims of the present invention. In order to provide a deep understanding of the present invention, the following description will include many specific details. The present invention can also be implemented without using these details. In addition, in order to avoid confusion or blurring the focus of the present invention, some specific details will be omitted in the description. It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

[0025] In the description of this embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the inventive product is usually placed when used. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0026] The terms “first”, “second”, etc. are only used for distinguishing descriptions and should not be understood as indicating or implying relative importance.

[0027] In the description of this embodiment, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this embodiment can be understood according to specific circumstances.

[0028] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0029] See also Figure 1-5As shown, the present invention discloses a nucleic acid amplification detection device, including a sealed shell 200 and a nucleic acid amplification reaction tube 100, wherein an inner cavity 210 is provided in the sealed shell 200, a plug-in portion 260 is provided on the sealed shell 200, a plug-in channel 261 is provided in the plug-in portion 260, and a puncture mechanism 250 is provided at the lower end of the plug-in channel 261. After the nucleic acid amplification reaction tube 100 is inserted into the plug-in channel 261, it is sealed and connected with the plug-in channel 261, and can be punctured by the puncture mechanism 250, so that the reaction liquid flows into the inner cavity 210 from the liquid outlet 252 of the puncture mechanism 250, and a liquid storage tank 220 and a detection test paper 240 are provided in the inner cavity 210, and the upper surface of the liquid storage tank 220 is sealed by a sealing film 221, and the detection test paper 240 is arranged on the sealing film 221. The closed film 221 can react with the reaction liquid. A pressing part 230 is provided in the inner cavity 210. The pressing part 230 is located at one end of the inner cavity 210 near the plug-in part 260. The pressing part 230 includes a pressing rod 231. The pressing rod 231 includes a pressing end 234 and a cone end 236. The middle part of the pressing rod 231 is rotatably sealed and connected to the side wall 222 of the liquid storage tank 220 through a pivot 232. The pressing end 234 is located in the inner cavity 210. The cone end 236 is located in the liquid storage tank 220. The cone end 236 is provided with a cone 237. The cone 237 can pierce the closed film 221. The pressing end 234 is provided with a button 235. After the reaction is completed, the button 235 is pressed to make the cone 237 of the cone end 236 tilt up and pierce the closed film 221. At the same time, a test result observation area 270 is also provided on the sealed housing 200 for users to observe the test results.

[0030] That is, the nucleic acid amplification detection device is mainly composed of a sealed housing 200 and a nucleic acid amplification reaction tube 100, wherein the sealed housing 200 is provided with an inner cavity 210, and the inner cavity 210 is Figure 1 The liquid reservoir 220, the sealing film 221 and the test paper 240 are provided in sequence, and the upper surface of the liquid reservoir 220 is sealed by the sealing film 221. When the detection device is not in use, the sealing film 221 can seal the liquid reservoir 220 to prevent the liquid in the liquid reservoir 220 from reacting with the reaction liquid on the test paper 240 and the nucleic acid amplification reaction tube 100, thereby ensuring the performance of the detection device. At this time, the plug-in portion 260 is located at the first position.

[0031] When the test strip 240 completes the test, the button 235 is pressed, so that the pressing rod 231 rotates around the pivot 232 to form a lever structure, so that the cone 237 of the cone end 236 tilts up, and then the cone 237 pierces the sealing film 221. The liquid in the liquid storage tank 220 reacts with the reaction liquid on the test strip 240 and in the nucleic acid amplification reaction tube 100, and the residual nucleic acid in the nucleic acid amplification detection device is completely removed. In this way, even if the nucleic acid amplification reaction tube 100 accidentally falls off or the nucleic acid amplification detection device is damaged in the subsequent process, causing the internal part to be exposed, no contamination will be caused.

[0032] In this embodiment, the liquid reservoir 220 may store a nucleic acid destroying agent, such as a sodium hypochlorite solution or a commercial DNA detergent solution. In other embodiments, the liquid reservoir may store other solutions as long as they can react with the reaction solution to avoid contamination by the reaction solution.

[0033] Furthermore, a plug-in portion 260 and a test result observation area 270 are provided on the upper surface of the sealed shell 200. The plug-in channel 261 of the plug-in portion 260 is configured to allow the nucleic acid amplification reaction tube 100 to be inserted. After the nucleic acid amplification reaction tube 100 is inserted into the plug-in channel 261, the inner cavity 210 is sealed, the puncturing mechanism 250 punctures the nucleic acid amplification reaction tube 100, and the reaction liquid flows into the inner cavity 210 from the liquid outlet 252 to react with the detection test paper 240 in the inner cavity 210.

[0034] The specific structure for sealing the inner cavity 210 may refer to any existing method in the prior art and will not be elaborated in the present invention. For example, the plug-in channel 261 may be shaped to match the nucleic acid amplification reaction tube 100. After the nucleic acid amplification reaction tube 100 is inserted into the plug-in channel 261, the surfaces of the two fit together to achieve the sealing of the inner cavity 210.

[0035] During the detection process, the inner cavity 210 is always kept sealed to prevent the amplification product from leaking out. After recording the test results, the closed membrane 221 can be pierced through the plug-in portion 260, and the test paper 240 can react with the nucleic acid destruction reagent to completely remove the residual nucleic acid in the nucleic acid amplification detection device. In this way, even if the nucleic acid amplification reaction tube 100 accidentally falls off or the nucleic acid amplification detection device is damaged in the subsequent process, causing the internal part to be exposed, no contamination will be caused.

[0036] With the above technical solution, when the test strip has completed the test, pressing the button causes the cone at the end of the cone to tilt up, puncturing the sealing membrane, so that the liquid in the liquid reservoir reacts with the reaction liquid on the test strip and in the nucleic acid amplification reaction tube, completely removing the residual nucleic acid in the nucleic acid amplification detection device. In this way, even if the nucleic acid amplification reaction tube accidentally falls off or the nucleic acid amplification detection device is damaged in the subsequent process, causing the internal part to be exposed, no contamination will be caused.

[0037] According to another specific embodiment of the present invention, see Figure 1-2 As shown, the pressing end 234 is provided with an elastic support member 233, and the elastic support member 233 is located below the button 235. By providing the elastic support member 233, the pressing end 234 can be continuously lifted up, and the cone 237 of the cone end 236 is prevented from tilting up under non-manual operation, ensuring that the cone 237 of the cone end 236 can only tilt up under manual pressure. In this embodiment, the elastic support member 233 is a spring, and in other embodiments, the elastic support member can also be other structures, such as a spring.

[0038] Further, in order to prevent misoperation, according to another specific embodiment of the present invention, see Figure 1-3 As shown, a flip cover 201 is provided at one end of the sealed housing 200, and the flip cover 201 is located above the button 235 to prevent the button 235 from being misoperated. Figure 1 As shown in the reverse direction of Y in the figure, the flip cover 201 is hinged to the sealed shell 200 and can be flipped up under the operation of the user. After being flipped up, the button 235 is exposed and then operated, preventing the button from being pressed during transportation or when it is not necessary.

[0039] According to another specific embodiment of the present invention, see Figure 2-3 As shown, the lower end of the plug-in portion 260 is provided with an elastic pressing member 262, which is used to press down the sealing film 221 and the test paper 240. The elastic pressing member 262 presses at least part of the test paper 240 into the liquid storage tank 220. The elastic pressing member 262 is not particularly limited, and can be a spring, a spring sheet, or an elastic protrusion.

[0040] Further, the specific structure for sealing the inner cavity 210 can refer to any existing method in the prior art, and will not be described in detail in the present invention. For example, the plug-in portion 260 can be shaped to match the nucleic acid amplification reaction tube 100. After the nucleic acid amplification reaction tube 100 is inserted into the plug-in portion 260, the surfaces of the two are in contact with each other, thereby sealing the inner cavity 210. According to another specific embodiment of the present invention, see Figure 1-2As shown, a sealing ring 263 made of an elastomer is provided inside the insertion channel 261, or a sealing ring 263 made of an elastomer is provided outside the side wall of the nucleic acid amplification reaction tube 100. By providing the sealing ring, it is possible to more effectively prevent the expansion product from leaking into the air.

[0041] It should be noted that the present invention does not limit the specific structure and manufacturing materials of the nucleic acid amplification reaction tube, and a reasonable selection can be made according to actual needs, as long as the nucleic acid amplification reaction tube can stably store the reaction solution and can be punctured by the puncturing mechanism.

[0042] For details, see Figure 4-5 Combined with Figure 1-3 As shown, in this embodiment, the nucleic acid amplification reaction tube 100 includes a side wall 110, a bottom wall 120 and an upper wall 130, and a sealed reaction liquid storage chamber 140 surrounded by the side wall 110, the bottom wall 120 and the upper wall 130. The side wall 110 is a cylindrical side wall, and its outer surface is provided with an external thread 150 for cooperating with the internal thread of the insertion channel 261 to achieve the sealing of the side wall 110 and the insertion channel 261 of the nucleic acid amplification reaction tube 100.

[0043] According to another specific embodiment of the present invention, in order to facilitate the nucleic acid amplification reaction tube 100 to be punctured by the puncturing mechanism 250 after being inserted into the nucleic acid amplification detection device, the bottom wall 120 of the nucleic acid amplification reaction tube 100 may be provided with a cut to facilitate the puncturing portion 251 of the puncturing mechanism 250 to puncture the nucleic acid amplification reaction tube 100.

[0044] Furthermore, since the bottom wall 120 of the nucleic acid amplification reaction tube 100 is provided with a cut, the nucleic acid amplification reaction tube 100 may be damaged at the position of the cut during the process of taking and placing before being inserted into the nucleic acid amplification detection device. Therefore, according to another specific embodiment of the present invention, the bottom wall 120 of the nucleic acid amplification reaction tube 100 is recessed into the reaction liquid storage chamber 140 to form a recess 121, and only the recess 121 is provided with a cut, which can effectively prevent the nucleic acid amplification reaction tube 100 from being damaged during the process of taking and placing.

[0045] According to another specific embodiment of the present invention, the general nucleic acid amplification reaction tube 100 is an open-cover structure, that is, the upper wall 130 is an openable cover. After the sample to be amplified and the reagents related to the amplification reaction system are placed in the nucleic acid amplification reaction tube 100, the cover is closed to achieve sealing. In addition, the upper part of the nucleic acid amplification reaction tube 100 can also be a closed structure directly, that is, the upper wall 130 and the side wall 110 are fixedly connected, or even directly integrally formed, and cannot be opened. When in use, a syringe with a fine needle penetrates the upper wall 130 of the nucleic acid amplification reaction tube 100, injects the reaction system, and then seals the breach with a sealing film or a wax drop with a higher melting point, so that the sealing of the nucleic acid amplification reaction tube 100 can be better achieved.

[0046] Furthermore, for some poor or backward areas, due to poverty, poor sanitary conditions, low hygiene awareness, malnutrition and other reasons, they have always been the hardest hit areas by infectious diseases. Infectious diseases have a high incidence rate and high mortality rate, and the high cost of treatment is difficult for ordinary families to afford. However, in these areas, advanced infectious disease testing methods cannot be popularized. The main reason is that most areas have difficulty in power supply and cannot operate large instruments; they cannot afford the cost of large medical equipment and the corresponding maintenance equipment costs; the site is limited; patients cannot afford the high cost of inspections, etc. The amplification reaction in the nucleic acid amplification reaction tube 100 needs to be carried out within a specific temperature range. In the above-mentioned area, it may be difficult for testers to obtain temperature constant temperature equipment and cannot be tested on the spot, which limits the immediacy of nucleic acid testing.

[0047] According to another specific embodiment of the present invention, the surface of the nucleic acid amplification reaction tube 100 is coated with at least two reversible temperature-sensitive color-changing materials. The color-changing temperature of the temperature-sensitive color-changing material can be set according to the actual situation, and the specific reversible temperature-sensitive color-changing material can be a commercially available product. For a certain amplification reaction, if the reaction temperature is required to be between the first temperature T1 and the second temperature T2, two temperature-sensitive color-changing materials can be selected to be coated on the surface of the nucleic acid amplification reaction tube 100, the color-changing temperature of the first temperature-sensitive color-changing material is the first temperature T1, and the color-changing temperature of the second temperature-sensitive color-changing material is the second temperature T2. In this way, when the amplification reaction is carried out, when the first temperature-sensitive material changes color and the second temperature-sensitive material does not change color, it indicates that the temperature is suitable for the amplification reaction in the nucleic acid amplification reaction tube 100. In this way, the nucleic acid amplification reaction tube 100 can be directly placed in a thermos bottle, and the amount of cold and hot water can be adjusted to control the temperature of the water in the thermos bottle, so as to maintain the progress of the amplification reaction, and no constant temperature equipment is required, and the sample can be tested anytime and anywhere.

[0048] For example, if the optimal reaction temperature is around 38 degrees, the temperature sensitive coating can be selected in two types, which are greater than 38 and less than 38, preferably 37 and 39 degrees. If the optimal reaction temperature is 63 degrees, the temperature sensitive coating can be selected as 62 and 64. The shape of the temperature sensitive coating can be any, but preferably Arabic numerals corresponding to the temperature, such as the temperature sensitive material that changes color at 38 degrees is displayed as "38". In this way, the temperature of the nucleic acid amplification reaction tube 100 can be more directly reflected.

[0049] Furthermore, when the nucleic acid amplification reaction tube 100 is used in amplification reaction systems at multiple different temperatures, multiple temperature-sensitive color-changing materials may be provided.

[0050] According to another specific embodiment of the present invention, the nucleic acid amplification reaction tube 100 of the present invention is a special nucleic acid amplification tube, and the side wall 110 of the nucleic acid amplification reaction tube 100 can be composed of a series of materials, which are preferably made of materials with good thermal conductivity, high strength and good fluidity, such as metals, alloys, thermally conductive plastics and organic composite materials, with a height of 1-3 cm, preferably 2 cm, and the general shape can be similar to that of ordinary nucleic acid amplification tubes, but there are some differences.

[0051] Although the present invention has been illustrated and described with reference to certain preferred embodiments of the present invention, it should be understood by those skilled in the art that the above is a further detailed description of the present invention in conjunction with specific embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. Those skilled in the art may make various changes in form and details, including making several simple deductions or substitutions, without departing from the spirit and scope of the present invention.

Claims

1. A nucleic acid amplification detection device, characterized in that: It comprises a sealed housing and a nucleic acid amplification reaction tube, wherein the surface of the nucleic acid amplification reaction tube is coated with at least two reversible temperature-sensitive color-changing materials, wherein: The sealed housing is provided with an inner cavity, a plug-in portion is provided on the sealed housing, a plug-in channel is provided in the plug-in portion, a puncture mechanism is provided at the lower end of the plug-in channel, after the nucleic acid amplification reaction tube is inserted into the plug-in channel, it is sealed and connected with the plug-in channel and can be punctured by the puncture mechanism, so that the reaction liquid flows into the inner cavity from the liquid outlet of the puncture mechanism. The inner cavity is provided with a liquid storage tank and a test paper, the upper surface of the liquid storage tank is sealed by a sealing film, and the test paper is arranged on the sealing film and can react with the reaction liquid. A pressing part is provided in the inner cavity, and the pressing part is located at one end of the inner cavity close to the plug-in part. The pressing part includes a pressing rod, and the pressing rod includes a pressing end and a cone end. The middle part of the pressing rod is rotatably sealed and connected to the side wall of the liquid storage tank through a pivot. The pressing end is located in the inner cavity, and the cone end is located in the liquid storage tank. The liquid storage tank stores a nucleic acid destructive reagent. The cone end is provided with a cone, and the cone can pierce the sealing film. The pressing end is provided with a button. After the reaction is completed, the button is pressed to cause the cone portion of the cone end to tilt up and pierce the closing film.

2. The nucleic acid amplification detection device according to claim 1, characterized in that: The pressing end is provided with an elastic support member, and the elastic support member is located below the button.

3. The nucleic acid amplification detection device according to claim 1, characterized in that: A flip cover is provided at one end of the sealed housing, and the flip cover is located above the button to prevent the button from being misoperated.

4. The nucleic acid amplification detection device according to claim 1, characterized in that: An elastic pressing piece is arranged at the lower end of the plug-in portion, and the elastic pressing piece is used to press down the closing film and the test paper.

5. The nucleic acid amplification detection device according to claim 1, characterized in that: A sealing ring made of an elastomer is arranged inside the insertion channel, or a sealing ring made of an elastomer is arranged outside the side wall of the nucleic acid amplification reaction tube.

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