Red blood cell cleaning device

Abstract

The present invention belongs to the technical field of medical equipment, and specifically relates to a cell cleaning device. It includes a physiological saline tube, a red blood cell tube, and a waste washing liquid tube. The physiological saline tube, the red blood cell tube, and the waste washing liquid tube adopt a three-chamber integrated structure design, wherein the red blood cell tube is arranged vertically below the physiological saline tube, and the waste washing liquid tube is wrapped around the outside of the physiological saline tube and the red blood cell tube. The physiological saline tube is connected to the red blood cell tube, and a gravity valve is arranged at the connection position. The red blood cell tube is connected to the waste washing liquid tube, and a biofilm is arranged at the connection position. The present invention is cleverly designed and reasonably structured, and provides an efficient cleaning device and method for cleaning red blood cells, which can save time and labor, and is suitable for wide promotion and use.

Description

Technical Field

[0001] The invention belongs to the technical field of medical equipment, and in particular relates to a cell cleaning device. Background Art

[0002] The function of red blood cell washing is to mix the red blood cells in physiological saline, centrifuge to remove residual white blood cells, and wash repeatedly, finally removing more than 98% of plasma, more than 90% of white blood cells and platelets, and also removing metabolites such as potassium, ammonia, lactic acid produced during the storage process, retaining more than 70% of red blood cells. The washed red blood cells can be used for neonatal hemolysis, and the red blood cells can be directly used in globulin tests, which greatly improves the accuracy of the test and avoids the influence of residual white blood cells, platelets and antibodies on the surface of red blood cells on red blood cells. The traditional washing method is to use a micropipette to repeatedly add samples, repeatedly centrifuge, and wash the red blood cells by continuously discarding the liquid. This process has many operating steps and takes a long time. When the sample volume is large, the work efficiency is low.

[0003] Therefore, it is necessary to solve the above problems. Summary of the invention

[0004] In view of the above problems, the present invention provides a red blood cell washing device, which adopts a three-chamber integrated structure design, so that the physiological saline tube, the red blood cell tube and the waste washing liquid tube are connected together, which greatly improves the washing efficiency. The technical solution adopted by the present invention is as follows:

[0005] A red blood cell washing device comprises a physiological saline tube, a red blood cell tube and a waste washing liquid tube, wherein the physiological saline tube, the red blood cell tube and the waste washing liquid tube adopt a three-chamber integrated structure design, wherein the red blood cell tube is arranged vertically below the physiological saline tube, the waste washing liquid tube is wrapped around the outside of the physiological saline tube and the red blood cell tube, the physiological saline tube is connected to the red blood cell tube, and a gravity valve is arranged at the connection position, and the red blood cell tube is connected to the waste washing liquid tube, and a biofilm is arranged at the connection position.

[0006] The physiological saline tube can hold 10 ml of physiological saline, and the gravity valve is arranged at the bottom of the physiological saline tube.

[0007] The red blood cell tube can hold 3 ml of red blood cells, and the bottom is composed of a biological membrane.

[0008] The waste washing liquid tube is "U"-shaped and can accommodate 10 ml of waste liquid.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] 1. The present invention adopts a three-chamber integrated structural design, so that the physiological saline tube, the red blood cell tube and the waste washing liquid tube are connected together. The entire operation process does not require multiple additions of physiological saline, but only needs to be placed in the centrifuge once, and the gravity after centrifugation is used to open the gravity valve at the bottom of the physiological saline, and the red blood cells and the physiological saline are fully mixed and washed by centrifugal force, and the waste liquid is discarded by the osmotic effect of the biofilm.

[0011] 2. The physiological saline tube of the present invention can hold 10 ml of physiological saline, and a gravity valve is provided at the bottom. The centrifugation interval is set to be constant, so that the volume of physiological saline entering the red blood cell tube is constant at 1-2 ml, which is diluted with the red blood cells below, and the concentration adjustment is accurate and convenient.

[0012] 3. The bottom of the red blood cell tube of the present invention is composed of a biofilm, and the waste cleaning solution is transferred into the waste cleaning solution tube by utilizing the osmotic effect of the biofilm. The operation is simple and convenient, and the work efficiency is greatly improved.

[0013] In summary, the present invention has an ingenious design and a reasonable structure, and provides an efficient cleaning device and method for cleaning red blood cells, which can save time and labor and is suitable for wide promotion and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0015] In the figure: 1 is a saline tube, 2 is a red blood cell tube, 3 is a waste washing solution tube, 4 is a gravity valve, and 5 is a biofilm. DETAILED DESCRIPTION

[0016] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0017] See also Figure 1The present invention provides a red blood cell washing device, which can solve the problem of multiple sample additions, multiple centrifugations, multiple dumpings and cumbersome operations using a micro-pipette during red blood cell washing. The device comprises a physiological saline tube 1, a red blood cell tube 2 and a waste washing liquid tube 3. The physiological saline tube 1, the red blood cell tube 2 and the waste washing liquid tube 3 adopt a three-chamber integrated structure design, wherein the red blood cell tube 2 is arranged vertically below the physiological saline tube 1, and the waste washing liquid tube 3 is wrapped around the outside of the physiological saline tube 1 and the red blood cell tube 2. The physiological saline tube 1 is connected with the red blood cell tube 2, and a gravity valve 4 is arranged at the connection position. The gravity valve 4 is arranged at the bottom of the physiological saline tube 1, the red blood cell tube 2 is connected with the waste washing liquid tube 3, and the bottom of the red blood cell tube is composed of a biofilm 5. The physiological saline tube 1 can accommodate 10 ml of physiological saline, the red blood cell tube 2 can accommodate 3 ml of red blood cells, and the waste washing liquid tube 3 is "U"-shaped and can accommodate 10 ml of waste liquid.

[0018] When washing cells, the device is placed in a centrifuge. At the end of centrifugation, when the gravity is greater than the centripetal force, the saline flushes the gravity valve 4 at the bottom of the saline tube 1, enters the red blood cell tube 2 below, and is fully mixed with the red blood cells under the action of centrifugal force. After the centrifugation, it passes through the biofilm 5 and enters the waste washing liquid tube 3. The three-chamber integrated operation is convenient and easy, and there is no need to transfer samples. The centrifugation interval is set constant to achieve a constant volume of saline entering the red blood cell tube 2. The waste washing liquid is transferred into the waste washing liquid tube 3 by the osmotic effect of the biofilm 5. This device converts the manual operation of washing cells into automation, simplifies the operation process, avoids mixing up specimens due to sample transfer, and the three structures are integrated and fully enclosed, pollution-free, and reduce operator contact. It greatly improves the accuracy, convenience, and adjustability of manual operations during cell washing, and improves the safety of operators.

[0019] Only the preferred embodiments of the present invention are described in detail above, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in this field without departing from the purpose of the present invention, and various changes should be included in the protection scope of the present invention.

Claims

1. A red blood cell cleaning device, characterized in that: The invention comprises a physiological saline tube (1), a red blood cell tube (2) and a waste washing liquid tube (3), wherein the physiological saline tube (1), the red blood cell tube (2) and the waste washing liquid tube (3) adopt a three-chamber integrated structure design, wherein the red blood cell tube (2) is arranged vertically below the physiological saline tube (1), and the waste washing liquid tube (3) is wrapped around the outside of the physiological saline tube (1) and the red blood cell tube (2); the physiological saline tube (1) is connected to the red blood cell tube (2), and a gravity valve (4) is arranged at the connection position; the red blood cell tube (2) is connected to the waste washing liquid tube (3), and a biofilm (5) is arranged at the connection position; The physiological saline tube (1) can hold 10 ml of physiological saline, and the gravity valve (4) is arranged at the bottom of the physiological saline tube (1); The red blood cell tube (2) can hold 3 ml of red blood cells, and the bottom is formed by a biological membrane (5); The waste washing liquid tube (3) is in a "U" shape and can accommodate 10 ml of waste liquid.

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