Magnetic bead cleaning and separation device, method and chemiluminescent immunoassay analyzer

Abstract

The present invention provides a magnetic bead cleaning and separation device, comprising a base, a transfer device, a liquid injection and discharge assembly, and a plurality of main magnetic components. The transfer device movably arranged on the base sequentially transfers reaction cups to corresponding operating positions on the base through a first parallel transfer track and a second parallel transfer track. A plurality of main magnetic components are arranged between the first transfer track and the second transfer track, so that each main magnetic component can act on the reaction cups in the first transfer track and the reaction cups in the second transfer track at its corresponding operating position. The same main magnetic component acts on different reaction cups on the first parallel transfer track and the second parallel transfer track to achieve the breaking up and aggregation of magnetic beads, thereby improving the utilization rate of the main magnetic component and the processing throughput of the magnetic bead cleaning and separation device, so that more reaction cups can be processed within the same operating time. The present invention also provides a corresponding chemiluminescent immunoassay analyzer and a corresponding magnetic bead cleaning and separation method.

Description

Technical Field

[0001] The present invention relates to the technical field of medical device cleaning, and in particular to a magnetic bead cleaning and separation device, a chemiluminescent immunoassay analyzer comprising the magnetic bead cleaning and separation device, and a corresponding magnetic bead cleaning and separation method. Background Art

[0002] Chemiluminescent immunoassay is a new type of ultra-microanalysis technology that combines luminescent analysis with immune reaction. This technology uses chemical or bioluminescent system as an indicator system for antigen-antibody reaction to quantitatively detect antigen or antibody. This method has both the high sensitivity of luminescent analysis and the high specificity of antigen-antibody reaction.

[0003] Magnetic bead washing and separation is an important preparatory step for chemiluminescent immunoassay. During the magnetic bead washing and separation process, it is necessary to break up the magnetic beads gathered in the reaction cup containing the cleaning solution to increase the cleaning effect of the magnetic beads. Traditional magnetic bead washing and separation devices mostly use mechanical or magnetic suction structures to achieve the dispersion of magnetic beads. The mechanical structure is relatively complex, large in size, high in production cost, and has a poor dispersion effect. The magnetic suction type mostly uses magnets arranged in a disc-shaped cavity to achieve the dispersion of magnetic beads. Under this structure, the interface for the reaction cup to enter and exit the magnetic separation device is relatively simple, and it is easy to define a multi-stage serial magnetic separation process through reciprocating cycles. However, due to the complex structure of the disc-shaped cavity itself, the many processes, and the high difficulty of processing, coupled with the fact that the deviation of the magnet position has a great influence on the magnetic separation cleaning effect, the disc-shaped cavity processing requirements of the magnet mounting seat are strict, resulting in a high cost of the disc-shaped magnetic separation structure.

[0004] In addition, due to the small size of the magnetic particles (about 1um in diameter), the process from dispersion to aggregation in the reaction cup takes a long time. For magnetic bead cleaning and separation devices, in order to improve the detection flux of the chemiluminescent immunoassay analyzer, multiple magnets are usually arranged in each stage of the magnetic bead cleaning process, and the aggregation time of the magnetic beads is dispersed into small time beats, shortening the time for the reaction cup to enter and exit the magnetic separation component. The shorter the time beat, the greater the test flux that the instrument can achieve, but the number of magnets that need to be arranged increases accordingly, and the diameter of the disc-type arrangement device is also larger, which further increases the manufacturing difficulty of the magnetic bead cleaning and separation device and reduces the reliability of the magnetic bead cleaning and separation device. Summary of the invention

[0005] The present invention provides an array-arranged magnetic bead cleaning and separation device, which reduces the volume of the magnetic bead cleaning and separation device by using a method in which at least two parallel tracks share a magnetic component.

[0006] The first aspect of the present invention provides a magnetic bead cleaning and separation device, comprising:

[0007] A base, wherein a plurality of operating positions are arranged on the base;

[0008] A transfer device, movably arranged on the base, the transfer device comprises at least two parallel, especially mutually parallel first transfer tracks and second transfer tracks, the first transfer track and the second transfer track are respectively provided with a plurality of carrying positions for carrying reaction cups, and the first transfer track and the second transfer track can respectively transfer the reaction cups to corresponding operation positions in sequence;

[0009] A liquid injection and discharge assembly is disposed above the first transfer track and the second transfer track, and the liquid injection and discharge assembly can be used to inject and discharge liquid from reaction cups at corresponding operating positions in the first transfer track and the second transfer track;

[0010] A plurality of main magnetic components are arranged between the first transfer track and the second transfer track, so that each of the main magnetic components can act on the reaction cups at the corresponding operating positions in the first transfer track and the second transfer track.

[0011] Furthermore, the first transfer track and the second transfer track may both extend linearly along a first direction in a horizontal plane, and a line connecting two magnetic pole ends of each main magnetic component may form a first angle with a vertical direction perpendicular to the horizontal plane.

[0012] Furthermore, the angle between the magnetic pole directions of two adjacent main magnetic components may be an obtuse angle.

[0013] Furthermore, the magnetic poles of two adjacent main magnetic components are in opposite directions.

[0014] Furthermore, a line connecting two magnetic pole ends of at least one, preferably multiple, and especially all of the main magnetic components can extend along a second direction within the horizontal plane, wherein the second direction is perpendicular to the first direction; or a line connecting two magnetic pole ends of at least one of the main magnetic components can extend along the vertical direction.

[0015] Further, the multiple operating positions may include a plurality of first operating positions arranged on the transfer path of the first transfer track and a plurality of second operating positions arranged on the transfer path of the second transfer track, the multiple first operating positions include a plurality of first main adsorption positions and the multiple second operating positions include a plurality of second main adsorption positions, the positions of the multiple first main adsorption positions on the transfer path of the first transfer track are opposite to the positions of the multiple second main adsorption positions on the transfer path of the second transfer track one by one, and a main magnetic component is arranged between each of the first main adsorption positions and the opposite second main adsorption position, so that each of the main magnetic components can act on the reaction cup at the corresponding first main adsorption position in the first transfer track and the reaction cup at the opposite second main adsorption position in the second transfer track.

[0016] Further, the multiple first operating positions may include at least one first filling position and at least one first liquid row position arranged alternately, the multiple second operating positions include at least one second filling position and at least one second liquid row position arranged alternately, the injection and drainage assembly includes a plurality of filling needles and a plurality of liquid drainage needles, one filling needle is respectively provided on each of the first filling positions and each of the second filling positions, and one liquid drainage needle is respectively provided on each of the first liquid row positions and each of the second liquid row positions, at least one first main adsorption position is provided between each of the first filling positions and the corresponding first liquid row positions, and at least one second main adsorption position is provided between each of the second filling positions and the corresponding second liquid row positions.

[0017] Furthermore, the multiple first operating positions may include a first cup loading position and a first cup unloading position, the first cup loading position is used to place the reaction cup to be transported on the first transfer track, and the first cup unloading position is used to take out the reaction cup on the first transfer track; the multiple second operating positions may include a second cup loading position and a second cup unloading position, the second cup loading position is used to place the reaction cup to be transported on the second transfer track, and the second cup unloading position is used to take out the reaction cup on the second transfer track.

[0018] Furthermore, at least one, preferably a plurality, and especially all of the main magnetic parts may be electromagnets and may be alternately supplied with currents in opposite directions to switch the magnetic poles.

[0019] Furthermore, the first transfer track and the second transfer track can be connected to form a continuous transfer track, so that the reaction cup on the first transfer track can be transferred to the second transfer track. This structure of the magnetic bead cleaning and separation device can achieve multi-stage cleaning without increasing the volume of the magnetic bead cleaning and separation device. In this embodiment, the multiple operating positions may include a cup loading position and a cup unloading position, the cup loading position is used to place the reaction cup to be transported on the continuous transfer track, and the cup unloading position is used to take out the reaction cup on the continuous transfer track. Preferably, the positions of the cup loading position and the cup unloading position coincide with each other.

[0020] Furthermore, the first transfer track and / or the second transfer track has two sides opposite to each other with respect to the extension direction thereof, the main magnetic component is arranged on one side, and a plurality of auxiliary magnetic components are arranged on the other side.

[0021] Furthermore, the first transfer track and the second transfer track both extend in a straight line along a first direction in a horizontal plane, and a line connecting the two magnetic pole ends of each main magnetic component forms a first angle with a vertical direction perpendicular to the horizontal plane, and a line connecting the two magnetic pole ends of the secondary magnetic component forms a second angle with the vertical direction, and the first angle and the second angle are different.

[0022] Furthermore, a line connecting two magnetic pole ends of the main magnetic component may be perpendicular to the vertical direction, and a line connecting two magnetic pole ends of the auxiliary magnetic component may be parallel to the vertical direction.

[0023] Further, one magnetic pole tip of the main magnetic component can be close to the cleaning area of ​​the reaction cup in the first transfer track at the corresponding operating position, and the corresponding other magnetic pole tip of the main magnetic component can be close to the cleaning area of ​​the reaction cup in the second transfer track, or one magnetic pole tip of the main magnetic component can be close to the cleaning area of ​​the reaction cup in the first transfer track and the second transfer track at the corresponding operating position, and the corresponding other magnetic pole tip of the main magnetic component is far away from the reaction cup in the first transfer track and the second transfer track; and / or one magnetic pole tip of the auxiliary magnetic component can be close to the cleaning area of ​​the reaction cup in the corresponding transfer track at the corresponding operating position, and the corresponding other magnetic pole tip of the auxiliary magnetic component is far away from the reaction cup in the corresponding transfer track.

[0024] Furthermore, the angle between the magnetic pole directions of two adjacent main magnetic components may be an obtuse angle, and the angle between the magnetic pole directions of two adjacent secondary magnetic components may be an obtuse angle.

[0025] Furthermore, the magnetic poles of two adjacent main magnetic components are in opposite directions, and / or the magnetic poles of two adjacent secondary magnetic components may be in opposite directions.

[0026] Furthermore, at least one, preferably a plurality, and especially all of the main magnetic components and preferably a plurality, and especially all of at least one of the secondary magnetic components are electromagnets and can be alternately supplied with currents of opposite directions to switch magnetic poles.

[0027] Furthermore, the transfer device may also include a third transfer track, which is arranged in parallel with the first transfer track and the second transfer track and has the same structure as the first transfer track and the second transfer track, and an auxiliary magnetic part is provided between the second transfer track and the third transfer track so that the auxiliary magnetic part can act on the reaction cup in the second transfer track and the reaction cup in the third transfer track.

[0028] Furthermore, the transfer device may also include a third transfer track and a fourth transfer track, which are parallel to the first transfer track and the second transfer track, especially arranged in parallel and have the same structure as the first transfer track and the second transfer track, and a plurality of main magnetic components are arranged between the third transfer track and the fourth transfer track, so that each of the main magnetic components can act on the reaction cups in the third transfer track and the reaction cups in the fourth transfer track at its corresponding operating position, and a secondary magnetic component is provided between the second transfer track and the third transfer track, so that the secondary magnetic component can simultaneously act on the reaction cups in the second transfer track and the reaction cups in the third transfer track.

[0029] The second aspect of the present invention also relates to a chemiluminescent immunoassay analyzer, which comprises an analysis device and the above-mentioned magnetic bead washing and separation device; the analysis device is used to analyze the magnetic beads washed by the magnetic bead washing and separation device.

[0030] A third aspect of the present invention provides a magnetic bead cleaning and separation method, comprising:

[0031] Respectively transporting reaction cups loaded with magnetic beads on at least two parallel first transport tracks and second transport tracks, wherein a plurality of main magnetic components are arranged between the first transport track and the second transport track;

[0032] When the reaction cups loaded with magnetic beads are transported to corresponding operating positions on the first transport track and the second transport track respectively, the multiple main magnetic parts can act on the reaction cups in the first transport track and the reaction cups in the second transport track to adsorb the magnetic beads in the reaction cups.

[0033] Further, the first transfer track and the second transfer track may be configured identically, so that the arrangement of the operating positions on the first transfer track is identical to the arrangement of the operating positions on the second transfer track, and the method may include:

[0034] Transporting the cuvettes loaded with magnetic beads respectively on at least two parallel first transport tracks and second transport tracks in the same manner;

[0035] When the reaction cups loaded with magnetic beads are transported to corresponding operating positions on the first transport track and the second transport track respectively, the multiple main magnetic parts can simultaneously act on the reaction cups in the first transport track and the reaction cups in the second transport track to adsorb the magnetic beads in the reaction cups.

[0036] Further, the first transport track and the second transport track may be connected to form a continuous transport track, so that the cuvette on the first transport track can be transported to the second transport track, and the method may include:

[0037] successively transporting the reaction cup loaded with magnetic beads on at least two parallel first transport tracks and second transport tracks;

[0038] When the reaction cup loaded with magnetic beads is transported to the corresponding operating position on the first transport track and the second transport track successively, the multiple main magnetic parts can act successively on the reaction cup when being transported on the first transport track and the reaction cup when being transported on the second transport track to adsorb the magnetic beads in the reaction cup.

[0039] Further, the first transfer track and / or the second transfer track has two sides opposite to each other in the extension direction thereof, the main magnetic member is disposed on one side, and a plurality of auxiliary magnetic members are disposed on the other side, and the method may include:

[0040] Transporting the cuvettes loaded with magnetic beads respectively on at least two parallel first transport tracks and second transport tracks in the same manner;

[0041] When the reaction cups loaded with magnetic beads are transported to corresponding operating positions on the first transport track and the second transport track respectively, the multiple secondary magnetic parts can act on the reaction cups in the first transport track and / or the reaction cups in the second transport track to adsorb the magnetic beads in the reaction cups.

[0042] Furthermore, the method can be implemented using the above-mentioned magnetic bead washing and separation device.

[0043] The above-mentioned magnetic bead cleaning and separation device, chemiluminescent immunoassay analyzer and magnetic bead cleaning and separation method are provided with a plurality of main magnetic parts between the first transport track and the second transport track which are arranged in parallel on the base, so that each main magnetic part can act on the reaction cup in the first transport track and the reaction cup in the second transport track at its corresponding operating position. Since the main magnetic parts can act on the two reaction cups on the two transport tracks accordingly, the utilization rate of the main magnetic parts is improved, thereby reducing the number of main magnetic parts and reducing the cost of the magnetic bead cleaning and separation device. In addition, the parallel multi-transport track setting increases the processing capacity of the magnetic bead cleaning and separation device, and can process more reaction cups in the same time, that is, increases the test throughput of the chemiluminescent immunoassay analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 It is a structural schematic diagram of an embodiment of the magnetic bead cleaning and separation device of the present invention;

[0045] Figure 2 It is a cross-sectional schematic diagram of an embodiment of the magnetic bead cleaning and separation device of the present invention;

[0046] Figure 3 is a cross-sectional schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0047] Figure 4 is a cross-sectional schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0048] Figure 5 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0049] Figure 6 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0050] Figure 7 It is a schematic diagram of the magnetic pole transformation of the electromagnet in the magnetic bead cleaning and separation device of the present invention;

[0051] Figure 8 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0052] Fig. 9 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0053] Fig.10 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0054] Fig.11 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0055] Fig.12 It is a schematic diagram for auxiliary explanation of the first angle and the second angle of the magnetic bead cleaning and separation device of the present invention;

[0056] Fig.13 It is a schematic diagram for assisting explanation of the adsorption positions of the main magnetic component and the auxiliary magnetic component of the magnetic bead cleaning and separation device of the present invention corresponding to the cleaning area;

[0057] Fig.14 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0058] Fig.15 It is a structural schematic diagram of another embodiment of the magnetic bead cleaning and separation device of the present invention;

[0059] Fig.16 It is a flow chart of the magnetic bead washing and separation method of the present invention. DETAILED DESCRIPTION

[0060] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0061] The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings). In the description of this application, it should be understood that the orientation or position relationship indicated by the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. are based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0062] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0063] See also Figure 1 and Figure 2 The magnetic bead cleaning and separation device 10 shown is used to clean the incubated and bound magnetic beads and separate the magnetic beads from free interfering substances. The magnetic bead cleaning and separation device according to the present invention is applied to a chemiluminescent immunoassay analyzer. The magnetic bead cleaning and separation device 10 includes a base 100, a transport device 200, a liquid injection and discharge assembly 300, and a main magnetic component 400. The base 100 is a supporting structure for the entire magnetic bead cleaning and separation device 10, and a plurality of operating positions 500 are arranged on the base 100. Figure 1 Indicated by a circle. The magnetic bead washing and separation device 10 performs corresponding operations on the reaction cup 20 containing magnetic beads and washing liquid at a specific operation position 500, such as injecting washing liquid, discharging washing liquid, adsorbing magnetic beads, putting in or taking out the reaction cup 20, etc. The transfer device 200 is movably arranged on the base 100, and the transfer device 200 at least includes a first transfer track 210 and a second transfer track 220 that are parallel, especially parallel to each other. The first transfer track 210 and the second transfer track 220 are respectively provided with a plurality of carrying positions that can be used to carry the reaction cup 20, and the first transfer track 210 and the second transfer track 220 can respectively transfer the reaction cup 20 to the corresponding operation position 500 in sequence. In other words, the reaction cup 20 is transferred to the corresponding operation position 500 along the transfer path of the first transfer track 210 and the second transfer track 220. The above-mentioned operation positions 500 are sequentially arranged on the base 100 along the first transfer track 210 and the second transfer track 220. The first transfer track 210 and the second transfer track 220 are provided with bearing positions arranged at intervals, the same number as the above-mentioned operating positions 500, and the intervals between the plurality of bearing positions are the same as the intervals between the plurality of operating positions 500, so as to ensure that each bearing position still corresponds to an operating position 500 after moving a certain distance on the transfer track. The liquid injection and discharge assembly 300 is installed on the corresponding operating position 500 above the first transfer track 210 and the second transfer track 220, and the liquid injection and discharge assembly 300 can be used to inject and discharge liquid from the cuvette 20 located at the corresponding operating position 500 in the first transfer track 210 and the second transfer track 220. A plurality of main magnetic components 400 are provided, and the plurality of main magnetic components 400 are all arranged between the first transfer track 210 and the second transfer track 220, so that each main magnetic component 400 can act on the cuvette 20 located at the corresponding operating position 500 in the first transfer track 210 and the second transfer track 220.

[0064] As a feasible method of the above-mentioned embodiment, the base 100 has a hollow cavity, and the first transfer track 210 and the second transfer track 220 are movably arranged in the hollow cavity of the base 100. The inner wall of the hollow cavity of the base 100 is also provided with a transfer groove 101 extending along the moving direction of the first transfer track 210 and the second transfer track 220, and the first transfer track 210 and the second transfer track 220 drive the reaction cup 20 to move in the transfer groove 101. The first transfer track 210 and the second transfer track 220 are movably arranged in the hollow cavity of the base 100, so as to realize the compact structure of the magnetic bead cleaning and separation device 10 provided by the present invention, and further reduce the volume of the magnetic bead cleaning and separation device 10 provided by the present invention. The reaction cup 20 is transported in the transfer groove 101 to prevent the reaction cup 20 from contacting with other components during the movement and being damaged.

[0065] In addition, a plurality of mounting grooves 102 are provided in the base 100, and the mounting grooves 102 are used to install the main magnetic component 400. The mounting grooves 102 are arranged between the first transfer track 210 and the second transfer track 220. The distribution positions of the mounting grooves 102 in the base 100 correspond one-to-one to the distribution positions of the main magnetic component 400 between the first transfer track 210 and the second transfer track 220.

[0066] See also Figure 2 , the main magnetic member 400 includes a first magnetic pole 401 and a second magnetic pole 402 that are opposite to each other. The main magnetic member 400 is disposed between the first transport track 210 and the second transport track 220, and its first magnetic pole 401 corresponds to the corresponding operation position 500 in the first transport track 210, and the second magnetic pole 402 corresponds to the corresponding operation position 500 in the second transport track 220. When the cuvette 20 in the first transport track 210 is transported to the operation position 500 corresponding to the first magnetic pole 401, the first magnetic pole 401 has an adsorption effect on the magnetic beads in the cuvette 20 on the corresponding operation position 500 in the first transport track 210, and the magnetic beads in the cuvette 20 in the first transport track 210 move to the side close to the first magnetic pole 401 and gather under the adsorption effect of the first magnetic pole 401. When the reaction cup 20 in the second transfer track 220 is transferred to the operating position 500 corresponding to the second magnetic pole 402, the second magnetic pole 402 also has an adsorption effect on the magnetic beads in the reaction cup 20 on the corresponding operating position 500 in the second transfer track 220. Under the adsorption effect of the second magnetic pole 402, the magnetic beads in the reaction cup 20 in the second transfer track 220 move to the side close to the second magnetic pole 402 and gather.

[0067] It is understandable that in the magnetic bead cleaning and separation device 10 of the present invention, the first magnetic pole 401 and the second magnetic pole 402 of the main magnetic part 400 are the N pole and the S pole of the magnet. However, the magnetic bead cleaning and separation device 10 of the present invention does not limit the correspondence between the first magnetic pole 401 and the second magnetic pole 402 and the N pole or the S pole. It is understandable that as long as the first magnetic pole 401 and the second magnetic pole 402 are two magnetic poles with opposite magnetic properties of the same main magnetic part 400, the magnetic beads in the reaction cup 20 in the corresponding operation position 500 in different transport tracks can be adsorbed respectively. That is, whether it is the reaction cup 20 of the operation position 500 located in the first transport track 210, or the reaction cup 20 of the operation position 500 located in the second transport track 220, the magnetic beads in the reaction cup 20 will be adsorbed by the same main magnetic part 400 corresponding to the operation position 500. This enables the same main magnetic part 400 to respectively realize the adsorption operation of the reaction cup 20 in two different transport paths. That is to say, the magnetic bead cleaning and separation device 10 of the present invention is provided with a plurality of main magnetic parts 400 between the parallel, especially parallel first transport track 210 and second transport track 220, so that each magnetic part 400 can simultaneously adsorb the magnetic beads in the reaction cups 20 on the two transport tracks, thereby improving the utilization rate of the main magnetic parts 400. At the same time, the first transport track 210 and the second transport track 220 share a plurality of main magnetic parts 400, which saves the number of magnetic parts used in the magnetic bead cleaning and separation device 10, making the structure of the magnetic bead cleaning and separation device 10 more compact. Therefore, the magnetic bead cleaning and separation device 10 of the present invention is reduced in size while saving costs. Furthermore, when both the first transfer track 210 and the second transfer track 220 carry reaction cups 20, the magnetic bead cleaning and separation device 10 of the present invention simultaneously transports the respective reaction cups 20 through the first transfer track 210 and the second transfer track 220 which are parallel to each other, and the magnetic beads in the transported reaction cups 20 can be adsorbed by multiple main magnetic parts 400 during the transportation on the first transfer track 210 or the second transfer track 220, so that the magnetic bead cleaning and separation device 10 of the present invention can realize the magnetic bead cleaning operation of double the number of reaction cups 20 within the same working time, thereby improving the processing capacity of the magnetic bead cleaning and separation device, and further improving the test throughput of the chemical immunoassay analyzer.

[0068] It should be pointed out that in the magnetic bead cleaning and separation device 10 provided by the present invention, the specific transfer path shape of the first transfer track 210 and the second transfer track 220 is not limited here. That is, the transfer path of the reaction cup 20 in the transfer device 200 can be a straight line, a circle, an arc or a combination of the former. The shape of the transfer path can be flexibly set based on the internal structure of the chemiluminescent immunoassay. Preferably, the first transfer track 210 and the second transfer track 220 are parallel to each other to better ensure that the main magnetic component 400 can act on the corresponding two reaction cups 20 at the same time.

[0069] Please look back Figure 1 In one embodiment, the transport path of the reaction cup 20 is in a straight line shape. Accordingly, the first transport track 210 and the second transport track 220 both extend in a straight line along the first direction 001 in the horizontal plane, and the line between the two magnetic pole ends of each main magnetic component 400 forms a first angle α with the vertical direction perpendicular to the horizontal plane. The first and second transport tracks 210 and 220 in a straight line shape have the same length on the transport path, so that the distance between the respective operating positions 500 on the first and second transport tracks 210 and 220 can also be set to be consistent, so as to uniformly control the transport distance of the reaction cup 20 between each operating position 500, so that the transport of the reaction cup 20 on the first transport track 210 and the transport of the reaction cup 20 on the second transport track 220 can proceed synchronously. The first angle α reflects that the two magnetic pole ends of the main magnetic component 400 correspond to the position of the reaction cup 20 on the first transport track 210 and the position of the reaction cup 20 on the second transport track 220, respectively.

[0070] like Figure 2 As shown, when the first transfer track 210 and the second transfer track 220 are both on the same horizontal plane, the two corresponding transfer cups 20 on different transfer paths are also on the same horizontal plane. At this time, the first angle α is preferably set to 90 degrees, that is, the line between the two magnetic pole ends of the main magnetic component 400 extends horizontally along the second direction 002 in the horizontal plane, and the second direction 002 is perpendicular to the first direction 001. That is, the main magnetic component 400 is horizontally arranged between the first transfer track 210 and the second transfer track 220. This ensures that the cleaning effect of the main magnetic component 400 on the reaction cups on both sides remains basically consistent. Of course, the line between the two magnetic pole ends of the main magnetic component 400 can also extend in the vertical direction.

[0071] In order to increase the attraction of the magnetic member 400 to the magnetic beads in the reaction cup 20, in one embodiment of the present invention, the first magnetic pole 401 of the main magnetic member 400 can be close to the cleaning area of ​​the reaction cup 20 on the first transport track 210 at the corresponding operation position, and the second magnetic pole 402 of the main magnetic member 400 can be close to the cleaning area of ​​the reaction cup 20 on the second transport track 220; or, the first magnetic pole 401 of the main magnetic member 400 is close to the cleaning area of ​​the reaction cup 20 on the first transport track 210 and the second transport track 220 at the corresponding operation position, and the second magnetic pole 402 of the main magnetic member 400 is set away from the reaction cup 20. The magnetic flux lines at the pole ends of the magnetic poles set in this way are denser, so the pole ends of the magnetic poles form a stronger adsorption force on the magnetic beads in the reaction cup 20. Even if the magnetic beads are on the side away from the magnetic poles of the main magnetic member 400, the main magnetic member 400 can still attract the magnetic beads in the shortest time, which is conducive to enhancing the dispersion effect and aggregation effect of the magnetic beads in the reaction cup 20 at the same time.

[0072] It should be noted that, in this embodiment, the reaction cup 20 includes a cleaning area. The cleaning area is an area in the reaction cup 20 containing cleaning liquid, which is generally located near the bottom of the reaction cup 20. Optionally, the cleaning area of ​​the reaction cup 20 is an area starting from the bottom of the reaction cup 20, and the length accounts for one-eighth of the total length of the reaction cup 20; or the cleaning area of ​​the reaction cup 20 is an area starting from the bottom of the reaction cup 20, and the length accounts for one-quarter of the total length of the reaction cup 20; or the cleaning area of ​​the reaction cup 20 is an area starting from the bottom of the reaction cup 20, and the length accounts for one-third of the total length of the reaction cup 20; or the cleaning area of ​​the reaction cup 20 is an area starting from the bottom of the reaction cup 20, and the length accounts for one-half of the total length of the reaction cup 20; or the cleaning area of ​​the reaction cup 20 can also be an area starting at a certain distance from the bottom of the reaction cup 20, and the length accounts for a specific proportion of the total length of the reaction cup 20. As another definition, the cleaning area of ​​the reaction cup 20 can also be the part below the liquid surface of the cleaning liquid. This embodiment does not limit the specific position of the cleaning area, as long as the magnetic member 400 can form a strong attraction to the magnetic beads. When the main magnetic member 400 is arranged horizontally, the first magnetic pole 401 and the second magnetic pole 402 of the main magnetic member 400 act on the same position of the cleaning area of ​​the cuvettes 20 on the first transport track and the second transport track. In one embodiment, the main magnetic member 400 is arranged so that the first magnetic pole 401 and the second magnetic pole 402 are both located at a position of 1 / 2 of the total height of the cleaning area of ​​the cuvettes on the first transport track and the second transport track, so as to better adsorb the magnetic beads in the respective cuvettes 20.

[0073] It is understandable that the present invention does not limit whether the first transfer track 210 and the second transfer track 220 of the magnetic bead cleaning and separation device 10 are on the same horizontal plane. Depending on the internal structure of the magnetic bead cleaning and separation device 10, especially the structure of the base 100, the first transfer track 210 and the second transfer track 220 may also be on different horizontal planes and arranged in parallel. For example, see Figure 3The first transfer track 210 and the second transfer track 220 are arranged in such a way that the line between the bottom of the reaction cup 20 in the first transfer track 210 and the bottom of the corresponding reaction cup 20 in the second transfer track 220 is set at an angle β with the vertical direction, that is, the two corresponding reaction cups 20 are transported in a staggered manner and parallel to each other. At this time, the main magnetic component 400 is arranged in such a way that the first angle α formed by the line between its first magnetic pole 401 and the second magnetic pole 402 and the vertical direction is the same as the angle β, so that the first magnetic pole 401 and the second magnetic pole 402 act on, that is, close to, the same position of the cleaning area of ​​the reaction cup 20 on the first transfer track 210 and the second transfer track 220. Alternatively, the main magnetic member 400 may be arranged such that the first angle α formed by the line connecting the first magnetic pole 401 and the second magnetic pole 402 and the vertical direction is 90°, and the first magnetic pole 401 and the second magnetic pole 402 act on different positions of the cleaning area of ​​the reaction cup 20 on the first transfer track 210 and the second transfer track 220, respectively. Figure 4 As shown, the first magnetic pole 401 of the main magnetic member 400 can be close to the side position of the cleaning area of ​​the reaction cup 20 in the first transfer track 210, and the corresponding second magnetic pole 402 of the main magnetic member 400 can be close to the bottom position of the cleaning area of ​​the reaction cup 20 in the second transfer track 220. Figure 3 and Figure 4 In the structure, the arrangement of each main magnetic member 400 may also be different, that is, the plurality of main magnetic members 400 may include a main magnetic member 400 arranged in a horizontal form (the first angle α is 90°) and / or a main magnetic member 400 arranged in a vertical form (the first angle α is 0°) and / or a main magnetic member 400 arranged at other angles. These structures are all embodiments claimed for protection of the magnetic bead cleaning and separation device 10 of the present invention.

[0074] During the cleaning and separation process of magnetic beads, the dispersion and reaggregation of magnetic beads is particularly important. The arrangement of the main magnetic component 400 affects the efficiency of the dispersion and aggregation of magnetic beads during the cleaning and separation process, and thus affects the effect and efficiency of the magnetic bead cleaning and separation device 10 in cleaning and separating magnetic beads. When the main magnetic component 400 is sequentially arranged between the first transport track 210 and the second transport track 220, the angle between the magnetic pole directions of two adjacent main magnetic components 400 is preferably an obtuse angle, for example, the magnetic pole directions of two adjacent main magnetic components 400 are opposite. It should be noted that, in the present embodiment, the magnetic pole direction of the main magnetic component 400 is a vector, and the magnetic pole direction of the magnetic component 400 specifically refers to a vector that points to the center of the S pole of the magnetic component 400 with the center of the N pole of the magnetic component 400 as the starting point. Still with Figure 2For example, when the first transfer track 210 and the second transfer track 220 are on the same horizontal plane, the first angle α of the main magnetic member 400 is set to 90 degrees. At this time, the magnetic pole direction angle of the two adjacent main magnetic members 400 is preferably set to 180 degrees, so that the magnetic pole directions of the two adjacent main magnetic members 400 are opposite (see Figure 1 ). That is to say, two adjacent main magnetic parts 400 are arranged in parallel in the same horizontal plane (that is, the connecting line between the two magnetic pole ends of each of the two adjacent main magnetic parts 400 is parallel in the same horizontal plane), and the magnetic poles of the two adjacent main magnetic parts 400 are in opposite directions. As a result, the magnetic poles of the two adjacent main magnetic parts 400 close to the same transport track are different. In this case, for any reaction cup 20 on the first transport track 210 or the second transport track 220, when it is successively transported to the operating position 500 corresponding to the two adjacent main magnetic parts 400, the magnetic beads in the reaction cup 20 at the previous operating position 500 corresponding to the previous main magnetic part 400 are adsorbed by the first magnetic pole 401 of the previous main magnetic part 400, and the magnetic beads gather toward the first magnetic pole 401. When the reaction cup 20 is transported to the next operation position 500 corresponding to the next main magnetic component 400, the magnetic beads in the reaction cup 20 are attracted by the second magnetic pole 402 of the next main magnetic component 400. At this time, because the magnetic properties of the first magnetic pole 401 of the previous main magnetic component 400 and the second magnetic pole 402 of the next main magnetic component 400 are different, the magnetic beads in the reaction cup 20 are first scattered by the second magnetic pole 402 of the next main magnetic component 400 during the process of the reaction cup 20 being transported from the previous operation position 500 to the next operation position 500, and then gathered again by the magnetic attraction of the second magnetic pole 402 of the next main magnetic component 400. In the process of the magnetic beads being "adsorbed-dispersed-gathered again", the magnetic beads in the reaction cup 20 experience a movement process of "being statically attracted-movement-dispersed-being statically attracted again" in the cleaning area. During this movement, the magnetic beads can be in full contact with the cleaning liquid in the cleaning area to ensure that the interfering substances attached to the magnetic beads are separated from the magnetic beads, thereby improving the cleaning effect of the magnetic beads by the magnetic bead cleaning and separation device 10. It should also be noted that the number of adjacent main magnetic parts 400 can be two, three, or other numbers set according to actual working conditions, which are not limited here.

[0075] It can be understood that the connection line between the pole ends of the two adjacent main magnetic parts 400 extending along the second direction 002 and having opposite magnetic poles is a preferred embodiment in which the angle between the magnetic pole directions of the two adjacent main magnetic parts 400 is an obtuse angle. In the remaining embodiments, as long as the angle between the two adjacent main magnetic parts 400 is set to an obtuse angle and the magnetic poles of the two are opposite, similar effects to the above embodiments can also be achieved. For example, the angle between the magnetic pole directions of the two adjacent main magnetic parts 400 is an obtuse angle, and the connection line between the two magnetic pole ends of each of the two adjacent main magnetic parts 400 is skew and parallel.

[0076] It is understandable that two adjacent main magnetic components 400 may also be arranged at different heights, that is, at different height positions close to the cleaning area of ​​the reaction cup 20 on the transfer track.

[0077] See also Figure 5 , the multiple operating positions 500 may include multiple first operating positions 510 arranged on the transfer path of the first transfer track 210 and multiple second operating positions 520 arranged on the transfer path of the second transfer track 220. The multiple first operating positions 510 may include multiple first main adsorption positions 511, and the multiple second operating positions 520 may include multiple second main adsorption positions 521. The positions of the multiple first main adsorption positions 511 on the transfer path of the first transfer track 210 are opposite to the positions of the multiple second main adsorption positions 521 on the transfer path of the second transfer track 220. That is, each first main adsorption position 511 is correspondingly provided with a second main adsorption position 521 with the same position in the second direction 002, wherein the second direction 002 is perpendicular to the first direction 001 extending from the first transfer track 210 and the second transfer track 220. A main magnetic member 400 is disposed between each first main adsorption position 511 and the opposite second main adsorption position 521, so that each main magnetic member 400 can act on the reaction cup 20 at the first main adsorption position 511 in the first transport track 210 and the reaction cup 20 at the opposite second main adsorption position 521 in the second transport track 220. In this way, the first main adsorption position 511 and the second main adsorption position 521 are arranged flush along the second direction 002, so that the length of the main magnetic member 400 can be close to the vertical distance between the first transport track 210 and the second transport track 220, and the outer length of the main magnetic member 400 is thus reduced to the minimum size.

[0078] like Figure 5 As shown, the multiple first operating positions 510 may also include at least one first filling position 512 and at least one first liquid row position 513 arranged alternately, and the multiple second operating positions 520 may also include at least one second filling position 522 and at least one second liquid row position 523 arranged alternately. The injection and drainage assembly 300 includes a plurality of filling needles 301 and a plurality of liquid drainage needles 302. An filling needle 301 is provided at each first filling position 512 and each second filling position 522, respectively, and a liquid drainage needle 302 is provided at each first liquid row position 513 and each second liquid row position 523, respectively. At least one first main adsorption position 511 is provided between each first filling position 512 and the corresponding first liquid row position 513, in this embodiment, two adjacent first main adsorption positions 511. At least one second main adsorption position 521 is provided between each second filling position 522 and the corresponding second liquid row position 523, in this embodiment, two adjacent second main adsorption positions 521. In Figure 5In the illustrated embodiment, the first liquid injection position 512 and the second liquid injection position 522 and the first liquid row position 513 and the second liquid row position 523 are positioned one by one on the first transfer track 210 and the second transfer track 220, just like the arrangement of the first main adsorption position 511 and the second main adsorption position 521. Thus, the reaction cups 20 that are simultaneously transported on the first transfer track 210 and the second transfer track 220 can be synchronously transported on their respective transport paths with the same transport rhythm and the same stepping distance. The synchronous transport action of the first transfer track 210 and the second transfer track 220 can also enable the first transfer track 210 and the second transfer track 220 to share the same power source to drive the reaction cups 20. Further, the liquid injection and discharge assembly 300 can also use the same liquid injection and discharge timing to simultaneously perform liquid injection and discharge operations on the reaction cups 20 in the first transfer track 210 and the transfer cups 20 in the second transfer track 220. Such a setting avoids the inconsistency of the transport rhythm of the reaction cup 20 in the first transport track 210 and the second transport track 220 due to the provision of multiple power sources, which in turn causes the injection needle 301 and the discharge needle 302 to perform injection and discharge operations respectively corresponding to different transport paths, resulting in defects such as complex mechanism control timing and reduced reliability. Furthermore, driving the first transport track 210 and the second transport track 220 with the same power source can save the volume and manufacturing cost of the magnetic bead washing and separation device 10, and thus can save the volume and manufacturing cost of the chemiluminescent immunoassay analyzer having the magnetic bead washing and separation device 10.

[0079] Since the magnetic bead cleaning and separation device 10 has the same transportation and cleaning process for the reaction cup 20 on the transportation paths of the first transportation track 210 and the second transportation track 220, the magnetic bead cleaning and separation process of the magnetic bead cleaning and separation device 10 is described here by taking the first transportation track 210 as an example. When the first transportation track 210 transports the reaction cup 20 to the first liquid injection position 512, the reaction cup 20 stays at the first liquid injection position 512 for a set time, and the injection needle 301 corresponding to the position injects the cleaning liquid into the reaction cup 20. After the injection needle 301 has finished injecting the liquid, the first transportation track 210 drives the reaction cup 20 to move to the corresponding first main adsorption position 511. The first transportation track 210 stays at each first main adsorption position 511 for a set time, so that each main magnetic part 400 attracts the magnetic beads in the reaction cup 20, and realizes the cleaning operation of quickly breaking up and quickly gathering the magnetic beads. The first transfer track 210 then transfers the cuvette 20 to the first row of liquid positions 513 corresponding to the first liquid injection position 512, and the cleaning liquid in the cuvette 20 is discharged by the liquid discharge needle 302 corresponding to the position of the first row of liquid positions 513. Then, the first transfer track 210 drives the cuvette 20 to move to the next first liquid injection position 512, and the cycle is repeated until the cuvette 20 moves to the last first row of liquid positions 513 to complete the process of magnetic bead cleaning and separation. On the first transfer track 210, a corresponding set of first liquid injection positions 512 and first row of liquid positions 513 and at least one first main adsorption position 511 arranged between the first liquid injection position 512 and the first row of liquid positions 513 form a first-stage cleaning operation for the magnetic beads in the cuvette 20. When multiple sets of first liquid injection positions 512 and first row of liquid positions 513 are alternately arranged, a multi-stage cleaning operation is formed for the magnetic beads in the cuvette 20 on the first transfer track 210. Of course, the magnetic bead cleaning and separation device 10 provided by the present invention can be used for single-stage magnetic bead cleaning and separation as well as for multi-stage magnetic bead cleaning and separation, and the present invention does not limit the specific application of magnetic bead cleaning and separation.

[0080] exist Figure 5In the illustrated embodiment, three groups of first liquid injection positions 512 and first liquid row positions 513 are provided in the first transfer track 210, and three groups of second liquid injection positions 522 and second liquid injection positions 523 are provided in the second transfer track 220. The reaction cups 20 on the first transfer track 210 and the second transfer track 220 will undergo three-stage magnetic separation and cleaning processes, namely, stage I, stage II, and stage III. The magnetic beads cleaned by multi-stage magnetic separation are not only conducive to enhancing the cleaning effect, but also can realize the assembly line cleaning of the magnetic beads, thereby improving the cleaning efficiency of the magnetic beads. Specifically, the plurality of first operating positions 510 include sequentially arranged stage I liquid injection positions 512, two stage I main adsorption positions 511, stage I liquid row positions 513, stage II liquid injection positions 512, two stage II main adsorption positions 511, stage II liquid row positions 513, stage III liquid injection positions 512, two stage III main adsorption positions 511, and stage III liquid row positions 513. The multiple second operating positions 520 include a sequentially arranged stage I liquid filling position 522, two stage I main adsorption positions 521, a stage I liquid discharge position 523, a stage II liquid filling position 522, two stage III main adsorption positions 521, a stage II liquid discharge position 523, a stage III liquid filling position 522, two stage III main adsorption positions 521, and a stage III liquid discharge position 523.

[0081] Also, see Figure 6 ,exist Figure 6 In the embodiment, the first transfer track 210 and the second transfer track 220 are respectively provided with a two-stage cleaning structure.

[0082] In addition, for the first transport track 210, the two main magnetic parts 400 located at the front and rear sides of the same first liquid injection position 512 or the same first liquid row position 513 along its transport path, or the two main magnetic parts 400 located between a group of first liquid injection positions 512 and first liquid row positions 513, can be regarded as two adjacent main magnetic parts 400, and are set based on the above embodiment that "the angle between the magnetic pole directions of the two adjacent main magnetic parts 400 is an obtuse angle", especially to make the magnetic properties of the two adjacent main magnetic parts 400 opposite. Thus, in the process of the reaction cup 20 being transported to the first main adsorption position 511 before and after the injection or discharge of the cleaning liquid, the magnetic beads in the reaction cup 20 also undergo a movement process of "being statically adsorbed-movement-dispersed-statically adsorbed again", which helps to enhance the cleaning effect of the magnetic bead cleaning and separation device 10 of the present invention. It can be understood that because the first transport track 210 and the second transport track 220 share the main magnetic part 400, the cleaning effect of the magnetic beads in the reaction cup 20 on the second transport track 220 is also enhanced.

[0083] In addition, Figure 5 and Figure 6As shown, the multiple first operating positions 510 also include a first cup loading position 514 and a first cup unloading position 515. The first cup loading position 514 is used to place the reaction cup 20 to be transported on the first transfer track 210, and the first cup unloading position 515 is used to take out the reaction cup 20 on the first transfer track 210; the multiple second operating positions 520 also include a second cup loading position 524 and a second cup unloading position 525. The second cup loading position 524 is used to place the reaction cup 20 to be transported on the second transfer track 220, and the second cup unloading position 525 is used to take out the reaction cup 20 on the second transfer track 220. It can be understood that the first cup loading position 514 and the second cup loading position 524 are respectively arranged at the most upstream of the respective transfer paths of the first transfer track 210 and the second transfer track 220, that is, the first cup loading position 514 is arranged upstream of the first liquid filling position 512 used for the first stage magnetic separation and cleaning operation in the first transfer track 210 along the transfer direction, and the second cup loading position 524 is also arranged upstream of the second liquid filling position 522 used for the first stage magnetic separation and cleaning operation in the second transfer track 220 along the transfer direction. The first cup unloading position 515 and the second cup unloading position 525 are respectively arranged at the most downstream of the respective transfer paths of the first transfer track 210 and the second transfer track 220, that is, the first cup unloading position 515 is arranged downstream of the first row of liquid positions 513 used for the last stage magnetic separation and cleaning operation in the first transfer track 210 along the transfer direction; the second cup unloading position 525 is arranged downstream of the second row of liquid positions 523 used for the last stage magnetic separation and cleaning operation in the second transfer track 220 along the transfer direction. Here, in Figure 6 In the embodiment, the first cup loading position 514 and the second cup loading position 524 can be configured as the same cup loading position, and the first cup unloading position 515 and the second cup unloading position 525 can also be configured as the same cup unloading position.

[0084] Further, such as Figure 5 As shown, a first substrate injection position 516 can also be set between the first row of liquid positions 513 and the first cup unloading position 515 of the first transport track 210 for the last cleaning operation. Correspondingly, the magnetic bead cleaning and separation device 10 also includes a substrate injection component 303. The substrate injection component 303 is arranged above the first substrate injection position 516, and the substrate injection component 303 is used to inject a luminescent substrate (enzymatic chemiluminescence) or an oxidant (direct chemiluminescence) into the reaction cup 20 located on the first substrate injection position 516. Correspondingly, a second substrate injection position 526 is also arranged between the second row of liquid positions 523 and the second cup unloading position 525 of the second transport track 220 for the last cleaning operation. A substrate injection component 303 is also correspondingly arranged above the second substrate injection position 526. As a feasible way, the substrate injection component includes a quantitative pump connected to the substrate, and the substrate injection component injects the substrate into the reaction cup 20 located at the substrate injection position 516 through the quantitative pump.

[0085] It should be noted that the magnetic bead cleaning and separation device 10 of the present invention does not limit the cup loading method or cup unloading method. At the first cup loading position 514, the second cup loading position 524, the first cup unloading position 515 and the second cup unloading position 525, the reaction cup 20 can be loaded or unloaded manually, or the reaction cup 20 can be transferred in or out by a cup loading and unloading mechanism (not shown in the figure). Different cup loading methods or cup unloading methods do not affect the implementation effect of the solution of sharing the main magnetic component 400 between the first transfer track 210 and the second transfer track 220 in the magnetic bead cleaning and separation device 10 of the present invention.

[0086] Regarding the implementation of the main magnetic member 400, a conventional magnet, such as a permanent magnet, or an electromagnet can be used to control the on and off of the current. In one embodiment, at least one main magnetic member 400 is an electromagnet. The main magnetic member 400 configured as an electromagnet can be alternately supplied with currents in opposite directions to alternately switch the magnetic poles of the main magnetic member 400. It can be understood that, see Figure 7 When the reaction cup 20 stays on the same first main adsorption position 511 or the second main adsorption position 521, and the main magnetic component 400 corresponding to the first main adsorption position 511 or the second main adsorption position 521 is an electromagnet, the residence time T of the reaction cup 20 on the first main adsorption position 511 or the second main adsorption position 521 is divided into two time periods: a first time period T1 and a second time period T2. Within the first time period T1, the current i1 in the main magnetic component 400 is controlled to be a positive current, at which time the magnetic pole of the main magnetic component 400 close to the reaction cup 20 is the first magnetic pole 401; within the second time period T2, the current in the main magnetic component 400 is controlled to be a current i2, and the current i1 and the current i2 are opposite to each other, at which time the magnetic pole of the main magnetic component 400 close to the reaction cup 20 is correspondingly transformed into the second magnetic pole 402. Since the electromagnet is alternately supplied with currents in opposite directions, the magnetic poles of the main magnetic part 400 close to the reaction cup 20 are changed accordingly, so that the magnetic beads in the reaction cup 20 also experience the movement process of "being statically adsorbed-movement-dispersed-statically adsorbed again" under the action of the changed magnetic poles, and the cleaning efficiency of the magnetic beads in this process is correspondingly improved, further enhancing the cleaning effect of the magnetic bead cleaning and separation device 10 of the present invention. Of course, if the residence time T of the reaction cup 20 on the same adsorption position 500 is further subdivided to increase the number of magnetic pole changes of the main magnetic part 400, the magnetic beads in the reaction cup 20 will experience a movement process of "being statically adsorbed-movement-dispersed-statically adsorbed again" during each magnetic pole conversion process of the main magnetic part 400, and the magnetic beads are repeatedly dispersed and finally gathered in the cleaning area, further increasing the contact time between the magnetic beads and the cleaning liquid, thereby improving the cleaning efficiency.

[0087] On the other hand, the main magnetic member 400 that changes its magnetic pole multiple times by means of an electromagnet has an effect of changing its magnetic pole equivalent to a scheme in which multiple first main adsorption positions 511 or multiple second main adsorption positions 521 are set in the transport path and the magnetic poles between two adjacent main magnetic members 400 are set oppositely. That is, the main magnetic member 400 that changes its magnetic pole multiple times by means of an electromagnet can save the number of the first main adsorption positions 511 and the second main adsorption positions 521 in the magnetic bead cleaning and separation device 10 of the present invention, reduce the volume of the magnetic bead cleaning and separation device 10, and is conducive to the miniaturization of the chemiluminescence immunoassay analyzer having the magnetic bead cleaning and separation device 10.

[0088] like Figure 8 As shown, the first transfer track 210 and the second transfer track 220 can be connected to form a continuous transfer track 202, so that the reaction cup 20 on the first transfer track 210 can be transferred to the second transfer track 220. Specifically, the first transfer track 210 includes a first end 211 and a second end 212 opposite to each other along the first direction 001, and the second transfer track 220 includes a third end 221 and a fourth end 222 opposite to each other along the first direction 001, wherein the first end 211 and the third end 221 are located on the same side of the first transfer track 210 and the second transfer track 220 along the first direction 001, and the second end 212 and the fourth end 222 are located on the other side of the first transfer track 210 and the second transfer track along the first direction 001. The transfer device 200 also includes a connecting track 201. One end of the connecting track 201 is connected to the second end 212 of the first transfer track 210, and the other end of the connecting track 201 is connected to the fourth end 222 of the second transfer track 220, so that the first transfer track 210 and the second transfer track 220 are connected to form a continuous transfer track 202. Thus, the cuvette 20 on the first transfer track 210 can be transferred to the second transfer track 220 by the connecting track 201. In this embodiment, the arrangement of the first liquid filling level 512 and the first liquid row level 513 on the first transfer track 210 and the second liquid filling level 522 and the second liquid row level 523 on the second transfer track 220 is different from that of the first liquid filling level 512 and the first liquid row level 513 on the first transfer track 210. Figure 5 In the embodiment shown, that is, the first filling level 512 and the second filling level 522 as well as the first row of liquid levels 513 and the second row of liquid levels 523 are not positioned one-to-one opposite to each other on the first transfer track 210 and the second transfer track 220, but the first filling level 512 and the second row of liquid levels 523 as well as the first row of liquid levels 513 and the second filling level 522 are positioned one-to-one opposite to each other on the first transfer track 210 and the second transfer track 220.

[0089] For the implementation of the connection track 201, the following embodiments may be adopted: Figure 8In the embodiment, the second end 212 and the fourth end 222 are connected by means of the same track as the first transfer track 210 and the second transfer track 220. In other embodiments, a mechanism such as a thumbwheel, a mechanical pusher, and a mechanical claw may also be used to realize the transfer operation of the reaction cup 20 from the first transfer track 210 to the second transfer track 220. The magnetic bead cleaning and separation device 10 of the present invention does not particularly limit the specific implementation of the connection track 201.

[0090] On the one hand, the first transfer track 210 and the second transfer track 220 are connected through the connecting track 201 to form a continuous transfer track 202, which can simplify the cup loading and unloading mechanism of the transfer device 200 and further reduce the volume of the magnetic separation cleaning device 10. That is, in the scenario of automatic cup loading and automatic cup unloading, there is no need to set up two cup loading mechanisms corresponding to the first cup loading position 514 and the second cup loading position 524, nor is there a need to set up two cup unloading mechanisms corresponding to the first cup unloading position 515 and the second cup unloading position 525. Instead, the first transfer track 210 and the second transfer track 220 on the continuous transfer track 202 share the same cup loading mechanism and the same cup unloading mechanism to achieve the purpose of simplifying the mechanism. In this case, only one cup loading position and one cup unloading position need to be set up, such as Figure 8 More preferably, as Fig. 9 As shown, the magnetic bead cleaning and separation device 10 of the present invention also provides an embodiment in which the cup loading position and the cup unloading position are shared. The first end 211 of the first transfer track 210 and the third end 221 of the second transfer track 220 extend toward each other and converge, so that the positions of the cup loading position and the cup unloading position overlap. In this embodiment, because the cup loading position and the cup unloading position are located at the same position, the cup loading mechanism and the cup unloading mechanism can be arranged more integrated, which is conducive to further reducing the volume of the magnetic bead cleaning and separation device 10.

[0091] On the other hand, the first transfer track 210 and the second transfer track 220 are connected by the connecting track 201 to form a continuous transfer track 202, so that the reaction cup 20 passes through the first transfer track 210 and the second transfer track 220 successively, so that the reaction cup 20 can undergo the magnetic separation and cleaning operations on the first transfer track 210 and the second transfer track 220 in turn, that is, the cooperation of the first transfer track 210 and the second transfer track 220 with a shorter length and the use of fewer injection and discharge liquid components 300 and main magnetic components 400 can be used to achieve multi-stage magnetic separation and cleaning of magnetic beads, so that the structure of the magnetic bead cleaning and separation device 10 is more compact. In particular, when the first transfer track 210 and the second transfer track 220 are connected to form a continuous annular transfer track, the multi-stage magnetic separation and cleaning of magnetic beads can be achieved with fewer injection and discharge liquid components 300 and main magnetic components 400, because in this case the injection and discharge liquid components 300 and the main magnetic components 400 can be reused multiple times. Figure 8In the embodiment shown, the reaction cup 20 first undergoes the I-stage and II-stage magnetic separation and cleaning processes on the first transport track 210, and then undergoes the III-stage and V-stage magnetic separation and cleaning processes on the second transport track 220. Figure 5 Compared to the embodiment shown, Figure 8 The illustrated embodiment can realize the same order of magnetic separation and cleaning processes without increasing the length of the transfer track. Of course, the processing throughput of the magnetic separation and cleaning device may be reduced accordingly.

[0092] Fig.10 This is a smaller structure embodiment of the magnetic bead cleaning and separation device 10 of the present application. Fig.10 In the embodiment, only one first liquid filling position 512 and one first liquid row position 513 are provided on the first transfer track 210, and only one second liquid filling position 522 and one second liquid row position 523 are provided on the second transfer track 220, and the cup loading position and cup unloading position of the continuous track 202 are shared. Thus, when only two main magnetic members 400 are provided, the reaction cup 20 realizes a two-stage magnetic separation and cleaning process on the first transfer track 210 and the second transfer track 220, respectively.

[0093] like Fig.11 As shown, the magnetic bead cleaning and separation device 10 may further include a plurality of auxiliary magnetic members 600 disposed on the outside of the first transport track 210 and / or the second transport track 220 and opposite to the position of the main magnetic member 400. The connecting line of the two magnetic pole ends of the auxiliary magnetic member 600 forms a second angle γ with the vertical direction, and the first angle α and the second angle γ are different, such as Fig.12 It should be noted that the two magnetic poles of the main magnetic component 400 and the auxiliary magnetic component 600 refer to the N pole and the S pole, and the line passing through the ends of the two magnetic poles refers to the imaginary line passing through the center of the end surface where the two magnetic poles of the main magnetic component 400 or the auxiliary magnetic component 600 are located. The vertical line is a vertical line in space. Fig.12 The first angle α and the second angle γ are respectively the angles formed between the line connecting the main magnetic member 400 and the auxiliary magnetic member 600 passing through the two magnetic pole ends and the vertical line. Preferably, the angle between the magnetic pole directions of two adjacent main magnetic members 400 is an obtuse angle, and the angle between the magnetic pole directions of two adjacent auxiliary magnetic members 600 is also an obtuse angle, especially opposite.

[0094] exist Fig.11In the illustrated embodiment, a first secondary magnetic member 610 and a second secondary magnetic member 620 are provided on the outer sides of the first transport track 210 and the second transport track 220 relative to the main magnetic member 400. Accordingly, the plurality of operation positions 510 include a first secondary adsorption position corresponding to the first secondary magnetic member 610, and the plurality of operation positions 520 include a second secondary adsorption position corresponding to the second secondary magnetic member 620. Taking the first transport track 210 as an example, after the reaction cup 20 is transported to the first main adsorption position 511, the magnetic beads contained therein gather toward the side close to the second transport track 220. Next, when the reaction cup 20 is transported along the transport path of the first track 210 to the first secondary adsorption position corresponding to the first secondary magnetic part 610, because the first secondary magnetic part 610 is located on the other side of the first transport track 210 relative to the main magnetic part 400, the magnetic beads in the reaction cup 20 move toward the side away from the second transport track 220 under the action of the first secondary magnetic part 610, so that the main magnetic part 400 and the first secondary magnetic part 610 have a pulling effect on the magnetic beads in the reaction cup 20, and the magnetic beads are fully in contact with the cleaning solution in the reaction cup 20 to ensure that the interfering substances attached to the magnetic beads are separated from the magnetic beads. The main magnetic part 400 and the secondary magnetic part 600 arranged at different angles can attract the magnetic beads in different directions, thereby increasing the movement dimension of the magnetic beads in the cleaning solution in the reaction cup 20, so as to fully and quickly break up the magnetic beads in the reaction cup 20, and finally increase the cleaning and separation effect of the magnetic beads and shorten the cleaning and separation time of the magnetic beads. The main magnetic member 400 and the auxiliary magnetic member 600 arranged at different angles can also gather the magnetic beads in different directions, thereby increasing the gathering speed of the magnetic beads and reducing the loss rate of the magnetic beads within the same operating time.

[0095] In the magnetic bead cleaning and separation device 10 provided by the present invention, the arrangement angles of the main magnetic member 400 and the secondary magnetic member 600 can be different from each other, or the arrangement angles of some main magnetic members 400 and some secondary magnetic members 600 can be the same. The change in the size of the arrangement angle between the main magnetic member 400 and the secondary magnetic member 600 can be either random or regular. The present invention does not limit the specific value of the arrangement angle between the main magnetic member 400 and the secondary magnetic member 600.

[0096] In one embodiment of the present invention, the main magnetic member 400 and the secondary magnetic member 600 arranged on the opposite sides of the transport path of the first transport track 210 or the second transport track 220 can pull the magnetic beads in the horizontal plane during the transport of the reaction cup 20. The first angle α of the multiple main magnetic members 400 and the second angle γ of the multiple secondary magnetic members 600 can ensure that the magnetic beads in the reaction cup 700 are repeatedly scattered and gathered in the set direction, enhance the degree of scattering and the gathering effect of the magnetic beads in the reaction cup 20, and ultimately increase the cleaning and separation effect of the magnetic beads and shorten the cleaning and separation time of the magnetic beads. In other embodiments of the present invention, the arrangement angles of the multiple main magnetic members 400 or the secondary magnetic members 600 arranged on the same side of the transport path are not exactly the same.

[0097] In one embodiment of the present invention, the line connecting the two magnetic pole ends of the main magnetic member 400 is perpendicular to the vertical direction, and the line connecting the two magnetic pole ends of the auxiliary magnetic member 600 is parallel to the vertical direction. That is, the first angle α is 90 degrees, and the second angle γ is 0 degrees. Fig.13 Schematic diagram, for the same cuvette 20 that can be transported to different operation positions 500, the positions of the main magnetic member 400 and the auxiliary magnetic member 600 close to the cleaning area of ​​the cuvette 20 are different. The two magnetic poles of the main magnetic member 400 are respectively close to the cleaning area of ​​the cuvette 20 in the first transport track 210 and the cleaning area of ​​the cuvette 20 in the second transport track 220, while one magnetic pole 601 of the auxiliary magnetic member 600 is close to the cleaning area of ​​the cuvette 20 in the corresponding transport track, and the other magnetic pole 602 of the auxiliary magnetic member 600 is far away from the cuvette 20 in the corresponding transport track. When the first angle α is 90 degrees and the second angle γ is 0 degrees, the main magnetic member 400 can be set so that the magnetic poles are close to the sides of the cleaning area of ​​the cuvette 20 on both sides, and the auxiliary magnetic member 600 can be set so that one magnetic pole is close to the bottom of the cleaning area of ​​the cuvette 20 in the corresponding transport track, and the other magnetic pole is far away from the cuvette in the corresponding transport track. By setting the main magnetic member 400 and the secondary magnetic member 600 to different height positions of the magnetic pole ends close to the cleaning area of ​​the reaction cup 20, the movement trajectory of the magnetic beads in the reaction cup 20 is further increased, and the cleaning effect is enhanced. As another feasible way, the arrangement of the main magnetic member 400 and the secondary magnetic member 600 can be reversed, the line connecting the two magnetic pole ends of the secondary magnetic member 600 is perpendicular to the vertical direction, and the line connecting the two magnetic pole ends of the main magnetic member 400 is parallel to the vertical direction. The two feasible ways can achieve the same effect. The present invention does not limit the specific arrangement of the main magnetic member 400 and the secondary magnetic member 600, as long as it can achieve the purpose of fully breaking up, pulling and gathering the magnetic beads in different directions.

[0098] As can be seen from the above drawings, preferably, a secondary magnetic member 600 can be provided near the first row of liquid positions 513 and the second row of liquid positions 523, that is, the first row of liquid positions 513 and the second row of liquid positions 523 can be simultaneously constituted as secondary adsorption positions. In the process of the discharge needle 301 sucking the cleaning liquid from the reaction cup 20 on the first row of liquid positions 513 and the second row of liquid positions 523 to discharge the liquid, the magnetic beads can be gathered at the bottom of the reaction cup 20 under the adsorption effect of the secondary magnetic member 600, so as to avoid the situation where the magnetic beads are suspended in the reaction cup 20 and may block the discharge needle 301. Of course, the secondary magnetic part 600 can also be arranged between each liquid injection position and the corresponding liquid discharge position, that is, at least one main magnetic part 400, preferably multiple adjacent main magnetic parts 400 and at least one secondary magnetic part 600, preferably multiple adjacent secondary magnetic parts 600 are arranged between each liquid injection position and the corresponding liquid discharge position, so that the magnetic beads are repeatedly pulled back and forth between the adsorption position of the main magnetic part 400 and the adsorption position of the secondary magnetic part 600 during the transportation of the reaction cup 20, increasing the movement path of the magnetic beads in the reaction cup 20, and achieving a better cleaning effect. Optionally, in this embodiment, the number of adjacent main magnetic parts 400 or secondary magnetic parts 600 can be two, three, or other numbers set according to actual working conditions. The present invention does not limit the specific number of adjacent main magnetic parts 400 or secondary magnetic parts 600, as long as the aggregation effect of the magnetic beads in the reaction cup 20 in the horizontal or vertical direction can be enhanced.

[0099] It is understandable that the secondary magnetic component 600 can also be implemented in the form of a magnet or an electromagnet, similar to the principle of the main magnetic component 400 using an electromagnet. Here, at least one main magnetic component 400 and at least one secondary magnetic component 600 can be implemented in the form of an electromagnet, and the main magnetic component 400 and the secondary magnetic component 600 can be alternately passed with currents in opposite directions, so that the main magnetic component 400 and the secondary magnetic component 600 can both achieve the effect of switching magnetic poles, and can also enhance the cleaning effect of the magnetic beads. Furthermore, the main magnetic component 400 and the secondary magnetic component 600 can also synchronously switch the direction of the current, so as to control the unified management of the current timing of the electromagnet drive circuit and simplify the control logic of the drive circuit.

[0100] It should be pointed out that in Fig.13 In the schematic diagram, the main magnetic member 400 and the secondary magnetic member 600 act on the same reaction cup 20, in order to describe the difference in the effect of the main magnetic member 400 and the secondary magnetic member 600 when adsorbing the magnetic beads in the reaction cup 20 from different directions and different vertical heights of the cleaning area of ​​the reaction cup 20. In the actual implementation process, the main magnetic member 400 and the secondary magnetic member 600 act on the same reaction cup 20 at different times and at different operating positions 500.

[0101] See also Fig.14, the transfer device 200 may further include a third transfer track 230. The third transfer track 230 is arranged on a side of the second transfer track 220 away from the first transfer track 210, and the third transfer track 230 is parallel to the first transfer track 210 and the second transfer track 220. The structure of the third transfer track 230 is the same as that of the first transfer track 210 and the second transfer track 220. The third transfer track 230 is also provided with a plurality of operating positions 530, and each operating position 530 is arranged opposite to the operating positions 510 and 520 on the first transfer track 210 and the second transfer track 220 in the second direction 002. The injection and discharge assembly 300 is also provided with an injection needle 301 and a discharge needle 302 at the third injection position 532 and the third discharge position 533 of the third transfer track 230. The cuvette 20 carried on the third transfer track 230 is sequentially transferred to each operating position 530 for injection, cleaning and discharge operations. A plurality of auxiliary magnetic components 600 are further provided between the second transfer track 220 and the third transfer track 230 so that the auxiliary magnetic components 600 can act on the reaction cups 20 in the second transfer track 220 and the reaction cups 20 in the third transfer track 230 respectively.

[0102] exist Fig.14 In the embodiment of the present invention, the second secondary magnetic component 620 is disposed near the second row of liquid level 523 of the second transport track 220, and accordingly, the second secondary magnetic component 620 can also act on the reaction cup 20 at the third row of liquid level 533 of the third transport track 230. During the liquid discharge process of the reaction cup 20 in the second transport track 220, the magnetic beads are adsorbed and aggregated by the secondary magnetic component 600, thereby preventing the magnetic beads from suspending in the reaction cup 20 and blocking the liquid discharge needle 302. Similarly, since the second secondary magnetic component 620 also acts on the reaction cup 20 at the third row of liquid level 533 of the third transport track 230 at the same time, the reaction cup 20 at the third row of liquid level 533 can also discharge more smoothly during the liquid discharge process due to the adsorption of the second secondary magnetic component 620, thereby preventing the liquid discharge needle 302 from being blocked. The sharing of the second auxiliary magnetic component 620 by the second transfer track 220 and the third transfer track 230 also saves the number of magnetic components used in the magnetic bead cleaning and separation device 10, saves costs and makes the structure of the magnetic bead cleaning and separation device 10 more compact.

[0103] Understandably, in Fig.14 In the embodiment, the third transfer track 230 is located on the side of the second transfer track 220 away from the first transfer track 210, and the third transfer track 230 and the second transfer track 220 share the second secondary magnetic component 620. In other embodiments, the third transfer track 230 can also be set on the side of the first transfer track 210 away from the second transfer track 220, and the third transfer track 230 and the first transfer track 210 share the first secondary magnetic component 610, which can also achieve the same effect.

[0104] See also Fig.15 , the transfer device 200 may also include a first transfer track 210, a second transfer track 220, a third transfer track 230 and a fourth transfer track 240. The third transfer track 230 and the fourth transfer track 240 are arranged parallel to the first transfer track 210 and the second transfer track 220. The structures of the third transfer track 230 and the fourth transfer track 240 are the same as those of the first transfer track 210 and the second transfer track 220, and a plurality of main magnetic components 400 are also arranged between the third transfer track 230 and the fourth transfer track 240. Each main magnetic component 400 can act on the reaction cup 20 in the third transfer track 230 and the reaction cup 20 in the fourth transfer track 240 at its corresponding operating position 500. Further, a second secondary magnetic component 620 is also arranged between the second transfer track 220 and the third transfer track 230, so that each second secondary magnetic component 620 can also act on the reaction cup 20 in the second transfer track 220 and the reaction cup 20 in the third transfer track 230.

[0105] Understandably, Fig.14 Similar to the embodiment, the third transfer track 230 and the fourth transfer track 240 can also be set on the side of the first transfer track 210 away from the second transfer track 220, so that the third transfer track 230 and the first transfer track 210 share the first secondary magnetic component 610, or the fourth transfer track 240 and the first transfer track 240 share the first secondary magnetic component 610, both of which can achieve the same effect.

[0106] exist Fig.14 and Fig.15 In the embodiment, the transfer device 200 is provided with a plurality of parallel tracks side by side along the second direction 002, so that the transfer device 200 is arranged in a matrix. Such an arrangement can improve the processing capacity of the magnetic bead cleaning and separation device 10, and realize batch cleaning operations of multiple reaction cups 20 at the same time. At the same time, because the main magnetic component 400 or the secondary magnetic component 600 can be shared between multiple rows of parallel tracks, the magnetic bead cleaning and separation device 10 can save the number of magnetic components used, improve the compactness of the structure of the magnetic bead cleaning and separation device 10, and facilitate the miniaturization of the chemiluminescent immunoassay analyzer.

[0107] Furthermore, the magnetic bead cleaning and separation device 10 further includes a magnetic shielding cover (not shown), which is provided at the outer edge of the main magnetic component 400 and / or the secondary magnetic component 600. The magnetic shielding cover can limit the magnetic effect of the magnetic component within the magnetic bead cleaning and separation device 10, thereby preventing the magnetic component from interfering with the electromagnetic magnetic elements near the magnetic bead cleaning and separation device 10, thereby allowing corresponding magnetic elements to be arranged near the magnetic bead cleaning and separation device 10 provided by the present invention, thereby increasing the space utilization rate of the magnetic bead cleaning and separation device 10 provided by the present invention, and thereby reducing the overall size of the chemiluminescent immunoassay analyzer.

[0108] The present invention also provides a chemiluminescent immunoassay analyzer, comprising an analysis device and the magnetic bead cleaning and separation device 10 described in any one of the above schemes. The analysis device is used to analyze the magnetic beads cleaned by the magnetic bead cleaning and separation device 10. It can be understood that the chemiluminescent immunoassay analyzer using the above magnetic bead cleaning and separation device 10 reduces the number of magnetic parts used and reduces the cost because of the sharing of multiple magnetic parts. At the same time, by setting up multiple parallel transfer tracks, the test throughput of the chemiluminescent immunoassay analyzer is increased because multiple reaction cups can be cleaned in batches at the same time. Furthermore, the chemiluminescent immunoassay analyzer also obtains a more compact internal structure.

[0109] See also Fig.16 , Fig.16 The specific process of a magnetic bead washing and separation method provided in this application is shown. It includes:

[0110] S10, transporting the cuvette 20 loaded with magnetic beads on at least two parallel first transport tracks 210 and second transport tracks 220, respectively, wherein a plurality of main magnetic components 400 are disposed between the first transport track 210 and the second transport track 220;

[0111] Specifically, as shown in the above-mentioned magnetic bead cleaning and separation device 10, the magnetic bead cleaning and separation method of the present application is carried out for the mutually parallel first transport track 210 and the second transport track 220. The first transport track 210 and the second transport track 220 are respectively used to transport the reaction cup 20 loaded with magnetic beads, and a plurality of main magnetic parts 400 are arranged between the first transport track 210 and the second transport track 220.

[0112] S20. When the reaction cups 20 loaded with magnetic beads are transported to the corresponding operating positions 500 on the first transport track 210 and the second transport track 220 respectively, the multiple main magnetic parts 400 can act on the reaction cups 20 in the first transport track 210 and the reaction cups 20 in the second transport track 220 to adsorb the magnetic beads in the reaction cups 20.

[0113] Specifically, by using the above structure, the cuvette 20 is transported on the first transport track 210 and the second transport track 220, respectively, so that the cuvette 20 on the first transport track 210 and the cuvette 20 on the second transport track 220 can pass by the multiple main magnetic parts 400. As a result, the magnetic beads in the cuvette 20 on the first transport track 210 can be adsorbed multiple times by the multiple main magnetic parts 400, thereby achieving the effect of washing the magnetic beads; the magnetic beads in the cuvette 20 on the second transport track 220 can also be adsorbed multiple times by the same multiple main magnetic parts 400, achieving the effect of washing the magnetic beads similar to that of the cuvette 20 on the first transport track 210.

[0114] The method achieves the effect of respectively acting on the reaction cups 20 in different transfer tracks by respectively transporting the reaction cups 20 on the first transfer track 210 and the second transfer track 220 parallel to each other, so that the two relative magnetic extreme ends of the multiple main magnetic parts 400 arranged between the first transfer track 210 and the second transfer track 220 respectively act on the reaction cups 20 in different transfer tracks, so that the two different transfer tracks can share the same set of magnetic parts to achieve the effect of magnetic bead cleaning in the reaction cups 20 transported by each of them, thereby improving the efficiency of magnetic bead cleaning and reducing the number of magnetic parts used.

[0115] In one embodiment, the first transfer track 210 and the second transfer track 220 are constructed in the same manner, so that the arrangement of the operating position 500 on the first transfer track 210 is the same as the arrangement of the operating position 500 on the second transfer track 220, and the above method includes:

[0116] S10a, transporting the cuvette 20 loaded with magnetic beads respectively on at least two parallel first transport tracks 210 and second transport tracks 220 in the same manner;

[0117] S20a. When the reaction cups 20 loaded with magnetic beads are simultaneously transported to the corresponding operating positions 500 on the first transport track 210 and the second transport track 220, the multiple main magnetic parts 400 can simultaneously act on the reaction cups 20 in the first transport track 210 and the reaction cups 20 in the second transport track 220 to adsorb the magnetic beads in the reaction cups 20.

[0118] Specifically, in this embodiment, because the first transport track 210 and the second transport track 220 have the same structure, the arrangement of the operating position 500 on the first transport track 210 and the arrangement of the operating position 500 on the second transport track 220 can also be the same. This method can synchronously perform the transport operation of the reaction cup 20 on the first transport track 210 and the second transport track 220 with the same arrangement of the operating position 500. At this time, the multiple main magnetic parts 400 can simultaneously act on the reaction cup 20 in the first transport track 210 and the reaction cup 20 in the second transport track 220 to adsorb the magnetic beads in the reaction cup 20.

[0119] The synchronously transported first transport track 210 and the second transport track 220 can share the same power source to drive the reaction cup 20. Furthermore, the same injection and discharge timing can be used for the synchronously transported reaction cup 20. Such a setting mechanism controls the unified management of the timing, and can also save the volume and manufacturing cost of the chemiluminescent immunoassay analyzer using this method.

[0120] In one embodiment, the first transport track 210 is connected to the second transport track 220 to form a continuous transport track 202, so that the cuvette 20 on the first transport track 210 can be transported to the second transport track 220. The above method includes:

[0121] S10b, successively transporting the cuvette 20 loaded with magnetic beads on at least two parallel first transport tracks 210 and second transport tracks 220;

[0122] S20b. When the reaction cup 20 loaded with magnetic beads is transported to the corresponding operating position 500 on the first transport track 210 and the second transport track 220 successively, the multiple main magnetic parts 400 can act successively on the reaction cup 20 when being transported on the first transport track 210 and the reaction cup 20 when being transported on the second transport track 220 to adsorb the magnetic beads in the reaction cup 20.

[0123] Specifically, in this embodiment, because the first transfer track 210 is connected to the second transfer track 220 to form a continuous transfer track 202, the reaction cup 20 on the first transfer track 210 can be transferred to the second transfer track 220. The same reaction cup 20 can be transferred on the first transfer track 210 and the second transfer track 220 successively. This method utilizes the same reaction cup 20 on the continuous transfer track 202 to successively pass through the two magnetic poles of the main magnetic component 400, so that the same reaction cup 20 can be successively adsorbed by the two magnetic poles of the main magnetic component 400 and cleaned, thereby improving the utilization rate of the main magnetic component 400.

[0124] In one embodiment, the first transfer track 210 and / or the second transfer track 220 has two sides opposite to each other in the extension direction thereof, a main magnetic member 400 is disposed on one side, and a plurality of secondary magnetic members 600 are disposed on the other side thereof, and the above method includes:

[0125] S10c, transporting the cuvette 20 loaded with magnetic beads respectively on at least two parallel first transport tracks 210 and second transport tracks 220 in the same manner;

[0126] S20c. When the reaction cup 20 loaded with magnetic beads is transported to the corresponding operating position 500 on the first transport track 210 and the second transport track 220 respectively, the multiple secondary magnetic parts 600 can act on the reaction cup in the first transport track 210 and / or the reaction cup in the second transport track 220 to adsorb the magnetic beads in the reaction cup 20.

[0127] Specifically, in this embodiment, when the reaction cup 20 is transported on the first transport track 210 and / or the second transport track 220, the magnetic bead cleaning operation is completed by the main magnetic component 400 and the auxiliary magnetic component 600. Because the auxiliary magnetic component 600 is located on the other side of the main magnetic component 400 relative to the first transport track 210 and / or the second transport track 220, the main magnetic component 400 and the auxiliary magnetic component 600 form a pulling effect on the magnetic beads in the reaction cup 20, and the magnetic beads are fully in contact with the cleaning solution in the reaction cup 20 to ensure that the interfering substances attached to the magnetic beads are separated from the magnetic beads.

[0128] Specifically, the magnetic bead washing and separation method of the present application can also be implemented using the above-mentioned magnetic bead washing and separation device 10. This method can obtain better magnetic bead washing and separation effects as the various embodiments of the magnetic bead washing and separation device 10 are developed, and the structure of the magnetic bead washing and separation device 10 is made more compact, saving the number of magnetic components used, and contributing to the miniaturization and cost control of the chemiluminescent immunoassay analyzer.

[0129] The features mentioned in the specification, claims and drawings above can be combined with each other as long as they are meaningful within the scope of the present invention. The advantages and features described for the magnetic bead cleaning and separation device 10 are applicable to the chemiluminescent immunoassay analyzer and the magnetic bead cleaning and separation method in a corresponding manner, and vice versa.

[0130] The above-described implementation methods do not constitute a limitation on the protection scope of the technical solution. Any modification, equivalent replacement and improvement made within the spirit and principle of the above-described implementation methods shall be included in the protection scope of the technical solution.

Claims

1. A magnetic bead cleaning and separation device, characterized in that: include: A base, wherein a plurality of operating positions are arranged on the base; A transfer device, movably arranged on the base, the transfer device comprises at least two parallel first transfer tracks and a second transfer track, the first transfer track and the second transfer track are respectively provided with a plurality of carrying positions for carrying reaction cups, and the first transfer track and the second transfer track can respectively transfer the reaction cups to corresponding operation positions in sequence; A liquid injection and discharge assembly is disposed above the first transfer track and the second transfer track, and the liquid injection and discharge assembly can be used to inject and discharge liquid from reaction cups at corresponding operating positions in the first transfer track and the second transfer track; A plurality of main magnetic components are arranged between the first transfer track and the second transfer track, so that each of the main magnetic components can act on the reaction cups at the corresponding operating positions in the first transfer track and the second transfer track.

2. The magnetic bead cleaning and separation device according to claim 1, characterized in that: The first transfer track and the second transfer track both extend linearly along a first direction in a horizontal plane, and a line connecting two magnetic pole ends of each main magnetic component forms a first angle with a vertical direction perpendicular to the horizontal plane.

3. The magnetic bead cleaning and separation device according to claim 2, characterized in that: The angle between the magnetic pole directions of two adjacent main magnetic parts is an obtuse angle.

4. The magnetic bead cleaning and separation device according to claim 3, characterized in that: The magnetic poles of two adjacent main magnetic components are in opposite directions.

5. The magnetic bead cleaning and separation device according to claim 2, characterized in that: A line connecting two magnetic pole ends of at least one of the main magnetic components extends along a second direction in the horizontal plane, the second direction being perpendicular to the first direction; or a line connecting two magnetic pole ends of at least one of the main magnetic components extends along the vertical direction.

6. The magnetic bead cleaning and separation device according to any one of claims 1 to 4, characterized in that: The multiple operating positions include multiple first operating positions arranged on the transfer path of the first transfer track and multiple second operating positions arranged on the transfer path of the second transfer track. The multiple first operating positions include multiple first main adsorption positions and the multiple second operating positions include multiple second main adsorption positions. The positions of the multiple first main adsorption positions on the transfer path of the first transfer track are opposite to the positions of the multiple second main adsorption positions on the transfer path of the second transfer track one by one. A main magnetic component is arranged between each of the first main adsorption positions and the opposite second main adsorption position, so that each of the main magnetic components can act on the reaction cup at the corresponding first main adsorption position in the first transfer track and the reaction cup at the opposite second main adsorption position in the second transfer track.

7. The magnetic bead cleaning and separation device according to claim 6, characterized in that: The multiple first operating positions include at least one first filling position and at least one first liquid row position arranged alternately, the multiple second operating positions include at least one second filling position and at least one second liquid row position arranged alternately, the injection and drainage assembly includes a plurality of filling needles and a plurality of liquid drainage needles, one filling needle is respectively provided on each of the first filling positions and each of the second filling positions, and one liquid drainage needle is respectively provided on each of the first liquid row positions and each of the second liquid row positions, at least one first main adsorption position is provided between each of the first filling positions and the corresponding first liquid row positions, and at least one second main adsorption position is provided between each of the second filling positions and the corresponding second liquid row positions.

8. The magnetic bead cleaning and separation device according to claim 6, characterized in that: The multiple first operating positions include a first cup loading position and a first cup unloading position, the first cup loading position is used to place the cuvette to be transported on the first transfer track, and the first cup unloading position is used to take out the cuvette on the first transfer track; the multiple second operating positions include a second cup loading position and a second cup unloading position, the second cup loading position is used to place the cuvette to be transported on the second transfer track, and the second cup unloading position is used to take out the cuvette on the second transfer track.

9. The magnetic bead cleaning and separation device according to any one of claims 1 to 4, characterized in that: At least one of the main magnetic components is an electromagnet and can be alternately supplied with currents in opposite directions to switch magnetic poles.

10. The magnetic bead cleaning and separation device according to any one of claims 1 to 4, characterized in that: One magnetic pole end of the main magnetic component is close to the cleaning area of ​​the reaction cup in the first transfer track at the corresponding operating position, while the other corresponding magnetic pole end of the main magnetic component is close to the cleaning area of ​​the reaction cup in the second transfer track; or one magnetic pole end of the main magnetic component is close to the cleaning area of ​​the reaction cup in the first transfer track and the second transfer track at the corresponding operating position, while the other corresponding magnetic pole end of the main magnetic component is far away from the reaction cup in the first transfer track and the second transfer track.

11. The magnetic bead cleaning and separation device according to any one of claims 1 to 4, characterized in that: The first transfer track and the second transfer track are connected to form a continuous transfer track, so that the reaction cup on the first transfer track can be transferred to the second transfer track.

12. The magnetic bead cleaning and separation device according to claim 11, characterized in that: The multiple operating positions include a cup loading position and a cup unloading position, the cup loading position is used to put the reaction cup to be transported on the continuous transfer track, and the cup unloading position is used to take out the reaction cup on the continuous transfer track.

13. The magnetic bead cleaning and separation device according to claim 12, characterized in that: The cup loading position and the cup unloading position overlap.

14. The magnetic bead cleaning and separation device according to claim 1, characterized in that: The first transfer track and / or the second transfer track has two sides opposite to each other with respect to the extension direction thereof, the main magnetic component is arranged on one side, and a plurality of auxiliary magnetic components are arranged on the other side.

15. The magnetic bead cleaning and separation device according to claim 14, characterized in that: The first transfer track and the second transfer track both extend in a straight line along a first direction in a horizontal plane, and a line connecting the two magnetic pole ends of each main magnetic component forms a first angle with a vertical direction perpendicular to the horizontal plane, and a line connecting the two magnetic pole ends of the auxiliary magnetic component forms a second angle with the vertical direction, and the first angle and the second angle are different.

16. The magnetic bead cleaning and separation device according to claim 15, characterized in that: A line connecting two magnetic pole ends of the main magnetic component is perpendicular to the vertical direction, and a line connecting two magnetic pole ends of the auxiliary magnetic component is parallel to the vertical direction.

17. The magnetic bead cleaning and separation device according to claim 14, characterized in that: One magnetic pole tip of the main magnetic component is close to the cleaning area of ​​the reaction cup in the first transfer track at the corresponding operating position, while the corresponding other magnetic pole tip of the main magnetic component is close to the cleaning area of ​​the reaction cup in the second transfer track, or one magnetic pole tip of the main magnetic component is close to the cleaning area of ​​the reaction cup in the first transfer track and the second transfer track at the corresponding operating position, while the corresponding other magnetic pole tip of the main magnetic component is far away from the reaction cup in the first transfer track and the second transfer track; and one magnetic pole tip of the auxiliary magnetic component is close to the cleaning area of ​​the reaction cup in the corresponding transfer track at the corresponding operating position, while the corresponding other magnetic pole tip of the auxiliary magnetic component is far away from the reaction cup in the corresponding transfer track.

18. The magnetic bead cleaning and separation device according to claim 14, characterized in that: The angle between the magnetic pole directions of two adjacent main magnetic parts is an obtuse angle, and the angle between the magnetic pole directions of two adjacent secondary magnetic parts is an obtuse angle.

19. The magnetic bead cleaning and separation device according to claim 18, characterized in that: The magnetic poles of two adjacent main magnetic components are in opposite directions, and / or the magnetic poles of two adjacent secondary magnetic components are in opposite directions.

20. The magnetic bead cleaning and separation device according to claim 14, characterized in that: At least one of the main magnetic component and at least one of the auxiliary magnetic component is an electromagnet and can be alternately supplied with currents in opposite directions to switch the magnetic poles.

21. The magnetic bead cleaning and separation device according to any one of claims 1 to 4, characterized in that: The transfer device also includes a third transfer track, which is arranged in parallel with the first transfer track and the second transfer track and has the same structure as the first transfer track and the second transfer track. A secondary magnetic part is provided between the second transfer track and the third transfer track so that the secondary magnetic part can act on the reaction cup in the second transfer track and the reaction cup in the third transfer track.

22. The magnetic bead cleaning and separation device according to any one of claims 1 to 4, characterized in that: The transfer device also includes a third transfer track and a fourth transfer track, which are arranged in parallel with the first transfer track and the second transfer track and have the same structure as the first transfer track and the second transfer track. A plurality of main magnetic components are arranged between the third transfer track and the fourth transfer track, so that each of the main magnetic components can act on the reaction cups in the third transfer track and the reaction cups in the fourth transfer track at its corresponding operating position, and a secondary magnetic component is arranged between the second transfer track and the third transfer track, so that the secondary magnetic component can act on the reaction cups in the second transfer track and the reaction cups in the third transfer track.

23. A chemiluminescent immunoassay analyzer, characterized in that: It comprises an analysis device and the magnetic bead washing and separation device according to any one of claims 1 to 22; the analysis device is used to analyze the magnetic beads washed by the magnetic bead washing and separation device.

24. A magnetic bead cleaning and separation method, characterized in that: The method is implemented using the magnetic bead cleaning and separation device according to any one of claims 1 to 22, and the method comprises the following steps: Respectively transporting reaction cups loaded with magnetic beads on at least two parallel first transport tracks and second transport tracks, wherein a plurality of main magnetic components are arranged between the first transport track and the second transport track; When the reaction cups loaded with magnetic beads are transported to corresponding operating positions on the first transport track and the second transport track respectively, the multiple main magnetic parts can act on the reaction cups in the first transport track and the reaction cups in the second transport track to adsorb the magnetic beads in the reaction cups.

25. The magnetic bead washing and separation method according to claim 24, characterized in that: The first transfer track and the second transfer track are configured identically, so that the arrangement of the operating positions on the first transfer track is identical to the arrangement of the operating positions on the second transfer track, and the method comprises: Transporting the cuvettes loaded with magnetic beads respectively on at least two parallel first transport tracks and second transport tracks in the same manner; When the reaction cups loaded with magnetic beads are transported to corresponding operating positions on the first transport track and the second transport track respectively, the multiple main magnetic parts can simultaneously act on the reaction cups in the first transport track and the reaction cups in the second transport track to adsorb the magnetic beads in the reaction cups.

26. The magnetic bead washing and separation method according to claim 24, characterized in that: The first transfer track is connected to the second transfer track to form a continuous transfer track, so that the cuvette on the first transfer track can be transferred to the second transfer track. The method includes: successively transporting the reaction cup loaded with magnetic beads on at least two parallel first transport tracks and second transport tracks; When the reaction cup loaded with magnetic beads is transported to the corresponding operating position on the first transport track and the second transport track successively, the multiple main magnetic parts can act successively on the reaction cup when being transported on the first transport track and the reaction cup when being transported on the second transport track to adsorb the magnetic beads in the reaction cup.

27. The magnetic bead washing and separation method according to any one of claims 24 to 26, characterized in that: The first transfer track and / or the second transfer track has two sides opposite to each other in the extension direction thereof, the main magnetic member is arranged on one side, and a plurality of auxiliary magnetic members are arranged on the other side, and the method comprises: Transporting the cuvettes loaded with magnetic beads respectively on at least two parallel first transport tracks and second transport tracks in the same manner; When the reaction cups loaded with magnetic beads are transported to corresponding operating positions on the first transport track and the second transport track respectively, the multiple secondary magnetic parts can act on the reaction cups in the first transport track and / or the reaction cups in the second transport track to adsorb the magnetic beads in the reaction cups.

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